Optika Italy B-510 Series User manual

Ver. 1.1 2019
INSTRUCTION MANUAL
B-510 Series
Model
B-510POL
B-510POL-I

Page 2
Table of Contents
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-510POL 4
5.2 B-510POL-I 5
6. Unpacking 6
7. Assembling 6
7.1 B-510POL 6
7.2 B-510POL-I 7
7.3 Microscope assembling 8
7.3.1 B-510POL 8
7.3.2 B-510POL-I 10
8. Transmittedlightbrighteldobservationprocedures 13
8.1 B-510POL 13
8.2 B-510POL-I 14
9. Useofthemicroscope(transmittedlightbrighteld) 15
9.1 Light intensity adjustment 15
9.2 Coarse focus tension adjustment 15
9.3 Coarse upper limit lever 15
9.4 Stage 16
9.5 Dioptric adjustment 16
9.6 Adjusting the interpupillary distance 16
9.7 Use of eye shields 16
9.8 Centering the condenser 17
9.9 Effectsoftheelddiaphragm 17
9.10 Aperture diaphragm 18
10. Polarized transmitted light observation procedures 19
10.1 B-510POL 19
10.2 B-510POL-I 20
11. Polarizedreectedlightobservationprocedures 21
11.1 B-510POL-I 21
12. Use of the microscope in polarized light 22
12.1 Centering of rotatable stage 22
12.2 Nosepiece centering 23
12.3 Checking the extinction of the light 23
12.3.1 B-510POL 23
12.3.2 B-510POL-I 24
12.4 Centeringofreectedlightdiaphragms 25
12.4.1Fielddiaphragm(FS) 25
12.4.2 Aperture diaphragm(AS) 26
12.5 Use of tint plates 26
12.6 Use of the Bertrand lens 27
12.7 Useofdiffusinglter 27
13. Microphotography 28
13.1 Use of C-mount cameras 28
13.2 Useofreexcameras 28
14. Maintenance 29
15. Troubleshooting 30
Equipment disposal 32

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the ope-
ration voltage of the equipment and that the lamp switch is in off position. Users should observe all safety regu-
lations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to
use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure safe
operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
PHOTO/TV PORT
DIOPTER
ADJUSTMENT
RING
5.1 B-510POL
NOSEPIECE
OBJECTIVES
APERTURE
DIAPHRAGM
FIELD DIAPHRAGM
OBSERVATION
HEAD
BERTRAND
LENS
ANALYZER
BERTRAND LENS
CENTERING SCREWS
TRANSMITTED LIGHT
POLARIZER
TINT PLATES
EYEPIECES
STAGE CENTERING
SCREWS
STAGE ROTATION
LOCKING SCREW
CONDENSER HEIGHT
ADJUSTMENT KNOB
COARSE
UPPER
LIMIT LEVER
MAIN SWITCH /
BRIGHTNESS ADJUSTMENT
KNOB
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
STAGE
CONDENSER CENTERING
SCREWS

Page 5
EYEPIECES
5.2 B-510POL-I
EYEPIECES
STAGE
CONDENSER
CENTERING
SCREWS
TENSION ADJUSTMENT
RING
STAGE CENTERING
SCREWS
REFLECTED LIGHT
ILLUMINATOR
REFLECTED LIGHT
POLARIZER
TRANSMITTED/REFLECTED
LIGHT SELECTOR
ANALYZER
DIFFUSING
FILTER
PHOTO/TV PORT
OBSERVATION
HEAD
BERTRAND
LENS
TINT PLATES
APERTURE
DIAPHRAGM
STAGE ROTATION
LOCKING SCREW
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
TRANSMITTED LIGHT
POLARIZER
FIELD DIAPHRAGM

Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the microscope box, the microscope parts are the following:
7.1 B-510POL
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Bertrand lens
⑥ Analyzer
⑦ Nosepiece centering screws
⑧ Dust cover
⑨ Tint plates
⑩ Tension adjustment tool
⑪ Allen wrench
⑫ Dummy slider
⑬ Power supply
③
⑫
④
⑤
⑥⑦
⑧
⑨
①
②
⑩⑪
⑬

Page 7
7.2 B-510POL-I
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Bertrand lens
⑥ Analyzer
⑦ Nosepiece centering screws
⑧ Dust cover
⑨ Tint plates
⑩ Tension adjustment tool
⑪ Allen wrench
⑫ Reflected light illuminator
⑬ Reflected light polarizer
⑭ Power supply
⑮ Diffusing filter
⑫
⑬
⑭
⑤
①
②
③
④
⑥⑦
⑧⑨
⑩⑪
⑮

Page 8
7.3 Microscope assembling
7.3.1 B-510POL
1. Insert the Bertrand lens ① on the frame and
lock the locking screw ② with the provided Allen
wrench. (Fig. 1)
2. Insert the optical head on the Bertrand lens and
lock the locking screw with the provided Allen
wrench. (Fig. 2)
• Hold the head with one hand during the locking
in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 3)
• One of the two eyepieces is equipped with
a crosshair for centering the entire optical
system. It is advisable to insert the eyepiece
with crosshair in the right eyepiece sleeve.
• Condenser is pre-installed in the factory. To
remove the condenser use an Allen wrench
1,5mm diam and operate on the locking screw
placed on the right side of the condenser holder.
4. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 4)
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
①
②

Page 9
5. Remove the dummy slider from Bertrand lens ③
and insert the analyzer ④. (Fig. 5 - 6)
6. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig. 7)
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
③
④

Page 10
7.3.2 B-510POL-I
1. Insert the reected light illuminator ① on the
frame and lock the locking screw ② with the
provided Allen wrench. (Fig. 8)
2. Connect the illuminator cable jack ③ to the socket
④ placed in the back side of the frame. (Fig. 9)
3. Insert the Bertrand lens ⑤ on the reected light
illuminator and lock the locking screw ⑥ with the
provided Allen wrench. (Fig. 9 - 10).
4. Insert the optical head on the Bertrand lens and
lock the locking screw with the provided Allen
wrench. (Fig. 11)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
FFig. 8ig. 8
FFig. 9ig. 9
FFig. 11ig. 11
FFig. 10ig. 10
①
②
③
④
⑤
⑥