Optika Italy B-510 Series User manual

Ver. 1.1 2019
INSTRUCTION MANUAL
B-510 Series
Model
B-510POL
B-510POL-I

Page 2
Table of Contents
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-510POL 4
5.2 B-510POL-I 5
6. Unpacking 6
7. Assembling 6
7.1 B-510POL 6
7.2 B-510POL-I 7
7.3 Microscope assembling 8
7.3.1 B-510POL 8
7.3.2 B-510POL-I 10
8. Transmittedlightbrighteldobservationprocedures 13
8.1 B-510POL 13
8.2 B-510POL-I 14
9. Useofthemicroscope(transmittedlightbrighteld) 15
9.1 Light intensity adjustment 15
9.2 Coarse focus tension adjustment 15
9.3 Coarse upper limit lever 15
9.4 Stage 16
9.5 Dioptric adjustment 16
9.6 Adjusting the interpupillary distance 16
9.7 Use of eye shields 16
9.8 Centering the condenser 17
9.9 Effectsoftheelddiaphragm 17
9.10 Aperture diaphragm 18
10. Polarized transmitted light observation procedures 19
10.1 B-510POL 19
10.2 B-510POL-I 20
11. Polarizedreectedlightobservationprocedures 21
11.1 B-510POL-I 21
12. Use of the microscope in polarized light 22
12.1 Centering of rotatable stage 22
12.2 Nosepiece centering 23
12.3 Checking the extinction of the light 23
12.3.1 B-510POL 23
12.3.2 B-510POL-I 24
12.4 Centeringofreectedlightdiaphragms 25
12.4.1Fielddiaphragm(FS) 25
12.4.2 Aperture diaphragm(AS) 26
12.5 Use of tint plates 26
12.6 Use of the Bertrand lens 27
12.7 Useofdiffusinglter 27
13. Microphotography 28
13.1 Use of C-mount cameras 28
13.2 Useofreexcameras 28
14. Maintenance 29
15. Troubleshooting 30
Equipment disposal 32

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the ope-
ration voltage of the equipment and that the lamp switch is in off position. Users should observe all safety regu-
lations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to
use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure safe
operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
PHOTO/TV PORT
DIOPTER
ADJUSTMENT
RING
5.1 B-510POL
NOSEPIECE
OBJECTIVES
APERTURE
DIAPHRAGM
FIELD DIAPHRAGM
OBSERVATION
HEAD
BERTRAND
LENS
ANALYZER
BERTRAND LENS
CENTERING SCREWS
TRANSMITTED LIGHT
POLARIZER
TINT PLATES
EYEPIECES
STAGE CENTERING
SCREWS
STAGE ROTATION
LOCKING SCREW
CONDENSER HEIGHT
ADJUSTMENT KNOB
COARSE
UPPER
LIMIT LEVER
MAIN SWITCH /
BRIGHTNESS ADJUSTMENT
KNOB
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
STAGE
CONDENSER CENTERING
SCREWS

Page 5
EYEPIECES
5.2 B-510POL-I
EYEPIECES
STAGE
CONDENSER
CENTERING
SCREWS
TENSION ADJUSTMENT
RING
STAGE CENTERING
SCREWS
REFLECTED LIGHT
ILLUMINATOR
REFLECTED LIGHT
POLARIZER
TRANSMITTED/REFLECTED
LIGHT SELECTOR
ANALYZER
DIFFUSING
FILTER
PHOTO/TV PORT
OBSERVATION
HEAD
BERTRAND
LENS
TINT PLATES
APERTURE
DIAPHRAGM
STAGE ROTATION
LOCKING SCREW
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
TRANSMITTED LIGHT
POLARIZER
FIELD DIAPHRAGM

Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the microscope box, the microscope parts are the following:
7.1 B-510POL
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Bertrand lens
⑥ Analyzer
⑦ Nosepiece centering screws
⑧ Dust cover
⑨ Tint plates
⑩ Tension adjustment tool
⑪ Allen wrench
⑫ Dummy slider
⑬ Power supply
③
⑫
④
⑤
⑥⑦
⑧
⑨
①
②
⑩⑪
⑬

Page 7
7.2 B-510POL-I
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Bertrand lens
⑥ Analyzer
⑦ Nosepiece centering screws
⑧ Dust cover
⑨ Tint plates
⑩ Tension adjustment tool
⑪ Allen wrench
⑫ Reflected light illuminator
⑬ Reflected light polarizer
⑭ Power supply
⑮ Diffusing filter
⑫
⑬
⑭
⑤
①
②
③
④
⑥⑦
⑧⑨
⑩⑪
⑮

Page 8
7.3 Microscope assembling
7.3.1 B-510POL
1. Insert the Bertrand lens ① on the frame and
lock the locking screw ② with the provided Allen
wrench. (Fig. 1)
2. Insert the optical head on the Bertrand lens and
lock the locking screw with the provided Allen
wrench. (Fig. 2)
• Hold the head with one hand during the locking
in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 3)
• One of the two eyepieces is equipped with
a crosshair for centering the entire optical
system. It is advisable to insert the eyepiece
with crosshair in the right eyepiece sleeve.
• Condenser is pre-installed in the factory. To
remove the condenser use an Allen wrench
1,5mm diam and operate on the locking screw
placed on the right side of the condenser holder.
4. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 4)
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
①
②

Page 9
5. Remove the dummy slider from Bertrand lens ③
and insert the analyzer ④. (Fig. 5 - 6)
6. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig. 7)
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
③
④

Page 10
7.3.2 B-510POL-I
1. Insert the reected light illuminator ① on the
frame and lock the locking screw ② with the
provided Allen wrench. (Fig. 8)
2. Connect the illuminator cable jack ③ to the socket
④ placed in the back side of the frame. (Fig. 9)
3. Insert the Bertrand lens ⑤ on the reected light
illuminator and lock the locking screw ⑥ with the
provided Allen wrench. (Fig. 9 - 10).
4. Insert the optical head on the Bertrand lens and
lock the locking screw with the provided Allen
wrench. (Fig. 11)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
FFig. 8ig. 8
FFig. 9ig. 9
FFig. 11ig. 11
FFig. 10ig. 10
①
②
③
④
⑤
⑥

Page 11
5. Insert both eyepieces into the tubes of the optical
head. (Fig. 12)
• One of the two eyepieces is equipped with
a crosshair for centering the entire optical
system. It is advisable to insert the eyepiece
with crosshair in the right eyepiece sleeve.
• Condenser is pre-installed in the factory. To
remove the condenser use an Allen wrench
1,5mm diam and operate on the locking screw
placed on the right side of the condenser holder.
6. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 13)
7. Insert reected light polarizer ⑦. (Fig. 14)
8. Remove the dummy slider from Bertrand lens ⑧
and insert the analyzer ⑨. (Fig. 15 - 16)
FFig. 13ig. 13
FFig. 12ig. 12
FFig. 14ig. 14
FFig. 15ig. 15
⑦
⑧

Page 12
9. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig.
17).
FFig. 16ig. 16
FFig. 17ig. 17
⑨

Page 13
8. Transmittedlightbrighteldobservationprocedures
Main switch /
brightness adjustment knob
(Used commands) (Chapter)
Nosepiece
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
Aperture diaphragm
Field diaphragm
Nosepiece
Coarse and fine focus knobs
Set the main switch to “ON” and adjust light intensity.
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
(5/9)
(5)
(5)
(5)
(9)
(9)
(9)
(9)
(9)
(9)
Coarse and fine focus knobs (5)
Main switch /
brightness adjustment knob (5/9)
8.1 B-510POL
Bertrand lens
Set the Bertrand lens ring on “O” (12)
Remove all filters from the light path Analyzer
Tint plates
(12)
(12)

Page 14
8.2 B-510POL-I
(Used commands) (Chapter)
Bertrand lens
Nosepiece
Nosepiece
Brightness adjustment knob
Coarse and fine focus knobs
Coarse and fine focus knobs
Set the main switch to “I” and adjust light intensity.
Set the Bertrand lens ring on “O”
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and
focus the specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
(12)
(5)
(5)
(5)
(5)
(5/9)
Remove all filters from the light path
Main switch
Brightness adjustment knob
(9)
(5/9)
Analyzer
Tint plates
(12)
(12)
Reected light illuminator
Set on “T” the light path selector on the reflected
light illuminator (12)
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
(9)
(9)
(9)
(9)
Aperture diaphragm
Field diaphragm
(9)
(9)

Page 15
9. Useofthemicroscope(transmittedlightbrighteld)
9.1 Light intensity adjustment
Operate on the light intensity adjustment knob to turn
ON / OFF the microscope and to increase / decrease
the illumination voltage ①. (Fig. 18)
• For B-510POL-I model only. There is a three-
position switch on the rear of the stand: position
“I” turns on the transmitted light, position “II” turns
on the uorescence and position “O” turns off the
microscope.
9.2 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-setted in the factory.
To modify the tension according to personal’s needs,
rotate the ring ② using the provided tool. (Fig. 19)
Clockwise rotation increases the tension.
If the tension is too loose, the stage could go lower
by itself or the focus easily lost after ne adjustment.
In this case, rotate the knob in order to increase the
tension .
9.3 Coarse upper limit lever
The upper limit knob has two functions: prevents
the contact between slide and objective and acts as
“focus memory”.
After focussing the specimen, pull the lever ③ toward
the front of the microscope and lock it. (Fig. 20). In
this way the focus upper limit is set. Now one can
lower the stage with coarse focus knob, replace the
specimen and raise again the stage up to the upper
limit: specimen will be in approximate focus and will
need a ne adjustment to get the proper focus.
Fine focus movement is not affected by the coarse
focus lock.
• To unlock, move the lever in the opposite
direction to the one used for the locking.
FFig. 18ig. 18
FFig. 19ig. 19
FFig. 20ig. 20
①
③
②

Page 16
9.4 Stage
1. The rotating stage accepts specimens on slide
(B-510POL) or opaque specimens (B-510POL-I).
2. It is possibe to lock the specimen once placed on
the stage using the stage clips ① . (Fig. 21)
3. After loosing the locking screw ② , the stage can
be rotated by 360°.
9.5 Dioptric adjustment
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ③ to compensate. (Fig. 22)
• Theadjustmentrangeis±5diopter.Thenumber
indicated on the adjustment ring graduation
should correspond to the operator’s dioptric
correction.
9.6 Adjusting the interpupillary distance
Observing with both eyes, hold the two eyepiece
prism assemblies. Rotate them around their common
axis until the elds of view coincide.
• The graduation on the interpupillary distance
indicator ④, pointed by the spot “.” on the
eyepiece holder, shows the distance between
theoperator’seyes.(Fig.23)
The range of the interpupillary distance is 48-75mm.
9.7 Use of eye shields
• Use with eyeglasses
Fold rubber eyeshields with both hands. Folded
eyeshields avoid scratching the lenses of eyeglasses.
(Fig. 24)
FFig. 21ig. 21
FFig. 22ig. 22
FFig. 23ig. 23
①
②
③
④
FFig. 24ig. 24

Page 17
• Use without eyeglasses
Raise eye shields and observe at the microscope
placing eyes to the shields, avoiding external light to
disturb the observation. (Fig. 25)
9.8 Centering the condenser
1. Place the specimen on the stage, insert 10x
objective into the light path and focus.
2. Insert the front lens of the swing-out condenser
①. (Fig. 26)
3. Rotate the eld diaphragm ring ②in
counterclockwise direction, to fully close the
diaphragm.
4. Rotate the condenser height adjustment knob ③
to focus the edges of the diaphragm.
5. Rotate the two centering screws ④to bring the
bright spot in the center of the eld of view.
6. Gradually open the diaphragm. The condenser
is centered when the diaphragm image is
symmetrical to the eld of view.
7. In normal use, open the diaphragm until it
circoscribes the eld of view.
9.9 Effectsoftheelddiaphragm
Field diaphragm adjusts the illuminated area to obtain
a high contrast image.
Set the diaphragm according to the objective in
use until it circoscribes the eld of view, in order to
eliminate unnecessary light to eyepieces.
FFig. 25ig. 25
FFig. 26ig. 26
②
③
①④

Page 18
9.10 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the
aperture diaphragm affects the image contrast.
Increasing or reducing this value one can vary
resolution, contrast and depth of focus of the
image.
• With low contrast specimens set the numerical
aperture value ① (printed on the condenser ring)
to about 70%-80% of the objective’s N.A. (Fig. 38)
If necessary, remove on eyepiece and, looking
into empty sleeve, adjust the condenser’s ring in
order to obtain an image like the one in g. 39.
Example: with objective PLAN 40x / 0,65 set the
scale to 0.65 x 0.8 = 0,52
FFig. 27ig. 27
FFig. 28ig. 28
①
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%

Page 19
10. Polarized transmitted light observation procedures
Main switch /
brightness adjustment knob
(Used commands) (Chapter)
Bertrand lens
Nosepiece
Main switch /
brightness adjustment knob
Coarse and fine focus knobs
Set the main switch to “ON” and adjust light intensity.
Set the Bertrand lens ring on “O”
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
(5/9)
(12)
(5)
(5)
(5)
(5)
(9)
(9)
(9)
(9)
(9)
(9)
(5/9)
10.1 B-510POL
Insert the analyzer into the light path Analyzer (12)
Analyzer (12)
Remove the sample from the optical path and check
for light extinction Polarizer (12)
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
Aperture diaphragm
Field diaphragm
Nosepiece
Coarse and fine focus knobs

Page 20
10.2 B-510POL-I
(Used commands) (Chapter)
Bertrand lens
Aperture diaphragm
Field diaphragm
Nosepiece
Brightness adjustment knob
Coarse and fine focus knobs
Set the main switch to “I” and adjust light intensity.
Set the Bertrand lens ring on “O”
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and
focus the specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
(12)
(5)
(5)
(9)
(9)
(9)
(9)
(9)
(9)
(5/9)
Insert analyzer into the light path
(9)
(5/9)
Analyzer (12)
Reected light illuminator
Set on “T” the light path selector on the reflected
light illuminator (12)
Nosepiece
Insert 10x objective into the light path (5)
Analyzer (12)
Remove the sample from the optical path and check
for light extinction Polarizer (12)
Main switch
Brightness adjustment knob
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
Coarse and fine focus knobs (5)