Optika Italy ACCESSORIES Series User manual

Ver. 2.1 2022
Model
M-870
ACCESSORIES Series
INSTRUCTION MANUAL

Page 2
Table of Contents
1. Warning 3
2. Safety Information 3
3. Package content 4
4. Unpacking 5
5. Intended use 5
6. Symbols and conventions 5
7. Instrument description 6
8. Assembling 7
8.1 B-1000MET 7
8.2 IM-5MET 7
9. DIC observation 8
9.1 DIC observation principle 8
9.2 B-1000MET 9
9.3 IM-5MET 10

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1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

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3. Package content
① DICPrism
①

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4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

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PRISM LEVER
PRISM
MOVEMENT KNOB
7. Instrument description
• The DIC prism has a lever that is used to lower or raise the position of the prism within the device.
• This position varies depending on which type of objective is being used.
• In the case of standard objectives the lever must be kept in, while in the case of LWD objectives the lever must
be pulled out.
• This is necessary to optimize observation in DIC.
• An incorrect prism position could lead to poor image quality or interference fringes within the image.
DIC PRISM USED WITH
STANDARD OBJECTIVES DIC PRISM USED WITH
LWD OBJECTIVES

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8. Assembling
FFig. 1ig. 1
FFig. 3ig. 3
①
FFig. 4ig. 4
③
FFig. 2ig. 2
• The DIC prism can be used on both the B-1000MET
series upright metallurgical microscope and the IM-
5MET inverted metallurgical microscope.
• The insertion direction of the prism is di󰀨erent for the
B-1000 and the IM-5MET.
8.1 B-1000MET
1. Loosen the clamping knob ① in the nosepiece. (Fig. 1)
2. Remove the dummy slider from the nosepiece.
3. Insert the DIC slider, with the prism lever ② facing right. (Fig.
2)
4. Clamp the prism by xing the clamping knob in the nosepiece
①.
②
8.2 IM-5MET
1. Loosen the clamping knob in the nosepiece ③. (Fig. 3)
2. Remove the dummy slider from the nosepiece.
3. Insert the DIC slider, with the prism lever ② facing left. (Fig.
4)
4. Clamp the prism by xing the clamping knob in the nosepiece
③.
②

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9.1 DIC observation principle
The DIC (Di󰀨erential Interference Contrast) observation method combines interference and polarization e󰀨ects and pro-
vides more contrasted images with a three-dimensional e󰀨ect.
The use of polarizer and analyzer are needed.
Light (Fig. 5), coming through the polarizer (a, is deected by the
brighteld cube (b) and enters the DIC prism (c). The prism serves
as beam splitter and divides the rays (d) into pairs vibrating at 90
degrees to each other with one set of rays traveling at a slower
speed than the other.
The light then goes to the specimen (e).
The light is reected back into the objective (Fig. 6) and once more
passes into the DIC prism (c). The net result is di󰀨erences in opti-
cal path, depending on the original path di󰀨erence, the specimen’s
e󰀨ect and the e󰀨ect of the prism serving as a beam recombiner in
the upward path.
The beams emerging from the prism cannot interfere with one an-
other until they are brought into the same plane of vibration by
means of the analyzer (g) which is in place above the brighteld
cube (b) as well as above the prism.
The net result of all of this is to produce a so-called “Nomarski-type
image” which appears three-dimensional with one side of individu-
al features appearing less bright than the other. This pseudo-3-di-
mensionality results from di󰀨erences in light intensity caused by
the optical path di󰀨erences described above.
FFig. 5ig. 5
9. DIC observation
FFig. 6ig. 6

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FFig. 5ig. 5
FFig. 8ig. 8
FFig. 9ig. 9
9.2 B-1000MET
• As with observation in polarized light, DIC observation
cannot be performed when the BF/DF switch on the mi-
croscope is in the ‘DF’ position.
1. Move the selector on the reected light illuminator in the
position “BF”.
2. Insert the polarizer “PO” with the inscription facing the user
(before remove the empty slider). (Fig. 7)
3. Move the polarizer “PO” into the light path until the slider
clicks into its position.
4. Insert analyzer “AN” with the inscription facing up (rst
removing the covering plates on the illuminator).
5. Move the rotatable analyzer “AN” into the light path until the
slider clicks into its position. (Fig. 8-9)
FFig. 7ig. 7
6. Put a at mirror on the stage and get into approximate focus
the mirror surface.
7. While observing into the eyepieces, rotate the scale of the
analyzer ①until you achieve the darkest image. (Fig. 10)
• Now the light extinction (so-called “crossed Nicol position”)
is achieved.
FFig. 10ig. 10
①

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FFig. 11ig. 11
FFig. 13ig. 13
FFig. 12ig. 12
①
8. Remove the mirror, place the specimen on the stage and
adjust the focus.
9. Adjust the background contrast by turning the prism movement
knob ② on the DIC slider. (Fig. 11)
10. When the prism movement knob ② on the DIC slider is turned,
the interference color in the background varies continuously
from the gray to magenta (from -100 to 600 nm). Set the
interference color which can provide best contrast with respect
to the specimen.
• If the background color is dark, the observation is similar to
darkeld.
• If the background color is grey, three-dimensional high contrast
images can be achieved rich in grey shades.
• If the background color is close to magenta, even small phase
variation can be observed as a change in color.
②
9.3 IM-5MET
• As with observation in polarized light, DIC observation
cannot be performed when the BF/DF switch on the mi-
croscope is in the ‘DF’ position.
1. Move the lever in “BF” position for brighteld illumination.
2. Insert the polarizer “PO” with the inscription facing the user.
(Fig. 12)
3. Move the polarizer “PO” into the light path until the slider
clicks into its position.
4. Insert analyzer “AN” with the inscription facing up. (Fig. 13)
5. Move the rotatable analyzer “AN” into the light path until the
slider clicks into its position.
6. Put a at mirror on the stage and get into approximate focus
the mirror surface.
7. While observing into the eyepieces, rotate the scale of the
analyzer ①until you achieve the darkest image. (Fig. 14)
• Now the light extinction (so-called “crossed Nicol position”)
is achieved.
8. Remove the mirror, put a specimen on the stage and focus
the specimen.
9. Adjust the background contrast by turning the prism movement
knob on the DIC slider ②. (Fig. 11)
10. When the prism movement knob ② on the DIC slider is turned,
the interference color in the background varies continuously
from the gray sensitive color to magenta sensitive color (from
-100 to 600 nm). Set the interference color which can provide
best contrast with respect to the specimen.
• If the background color is dark, the observation is similar to
darkeld.
• If the background color is grey, three-dimensional high contrast
images can be achieved rich in grey shades.
• If the background color is close to magenta, even small phase
variation can be observed as a change in color.
FFig. 14ig. 14
①