Optika Italy B-380 Series User manual

Model
B-382PL-ALC
B-383PL
B-382PLI-ALC
B-383PLI
B-382PH-ALC
B-383PH
B-382PHI-ALC
B-383PHI
B-383FL
B-383LD
B-380 Series
INSTRUCTION MANUAL
Ver. 6.4 2022

Page 2
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 B-382PL-ALC / B-382PLI-ALC 4
3.2 B-383PL / B-383PLI 4
3.3 B-382PH-ALC / B-382PHI-ALC 5
3.4 B-383PH / B-383PHI 5
3.5 B-383FL 6
3.6 B-383LD 6
4. Unpacking 7
5. Intended use 7
6. Symbols and conventions 7
7. Instrument description 8
7.1 B-382PL-ALC / B-382PLI-ALC 8
7.2 B-383PL / B-383PLI 9
7.3 B-382PH-ALC / B-382PHI-ALC 10
7.4 B-383PH / B-383PHI 11
7.5 B-383FL 12
7.6 B-383LD 14
8. Assembling 15
8.1 Assembling the microscope 15
8.2 Field Diaphragm (Optional) 16
8.3 Polarizing set (optional) 16
9. Brightfield transmitted light observation procedures 18
10. Use of the microscope (Brightfield transmitted light) 19
10.1 Light intensity adjustment 19
10.2 Adjust the interpupillary distance 19
10.3 Diopter adjustment 19
10.4 Coarse focus tension adjustment 19
10.5 Focus lock lever 20
10.6 Stage 20
10.7 Condenser centering 20
10.7.1 Centeringwithoutelddiaphragm 20
10.7.2 Centeringwithelddiaphragm 21
10.8 E󰀨ectsoftheelddiaphragm 21
10.9 Aperture diaphragm 21
10.10 Use of oil immersion objective 22
10.11 Use of ALC system 22
10.12 Use of the polarizer (optional) 22
11. Use of universal condenser for Brightfield/Darkfield/Phase Contrast 23
11.1 Brighteldobservation(BF) 23
11.2 Darkeldobservation(DF) 23
11.3 Phase contrast observation (PH) 24
11.4 Useofthegreenlter 25
12. Fluorescence reflected light observation procedures (B-383FL) 26
13. Fluorescence reflected light observation procedures (B-383LD) 26
14. Use of the microscope (Fluorescence reflected light) 27
14.1 Assembling procedure (B-383FL) 27
14.2 Assembling procedure (B-383LD) 27
14.3 HBO bulb assembling (B-383FL) 28
14.4 Centering the HBO bulb (B-383FL) 30
14.5 Use of the microscope (B-383FL) 32
14.6 Use of the microscope (B-383LD) 32
14.7 Use of the shutter 33
14.8 Use of the light excluding plate 33
15. Simultaneous observation Phase Contrast + Fluorescence (B-383FL) 34
16. Microphotography 35
16.1 Installing the C-mount adapter 35
16.2 Useofreexcameras 35
17. Maintenance 36
18. Troubleshooting 37
Equipment disposal 39
Table of content

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4
3. Package content
3.1 B-382PL-ALC / B-382PLI-ALC
3.2 B-383PL / B-383PLI
① Frame
② Objectives
③ ALCobservationhead
④ Eyepieces
⑤ Tensionadjustmenttool
⑥ Dustcover
⑦ Powersupply
⑧ Immersionoil
①
③
④
⑤
⑥
⑦
⑧
②
① Frame
② Objectives
③ Trinocularobservationhead
④ Eyepieces
⑤ Tensionadjustmenttool
⑥ Dustcover
⑦ Powersupply
⑧ Immersionoil
①
③
④
⑤
⑥⑦
⑧
②

Page 5
3.3 B-382PH-ALC / B-382PHI-ALC
3.4 B-383PH / B-383PHI
① Frame
② Objectives
③ ALCobservationhead
④ Eyepieces
⑤ Tensionadjustmenttool
⑥ Dustcover
⑦ Powersupply
⑧ Immersionoil
⑨ Greenlter+lterholder
⑩ Centeringtelescope
①
③
④
⑤
⑥⑦
⑧
②
⑨
⑩
① Frame
② Objectives
③ Trinocularobservationhead
④ Eyepieces
⑤ Tensionadjustmenttool
⑥ Dustcover
⑦ Powersupply
⑧ Immersionoil
⑨ Greenlter+lterholder
⑩ Centeringtelescope
①
③
④
⑤
⑥⑦
⑧
②
⑨
⑩

Page 6
3.5 B-383FL
3.6 B-383LD
① Frame
② Objectives
③ Trinocularobservationhead
④ HBOuorescenceilluminator
⑤ Fluorescencepowersupply+powercord
⑥ Eyepieces
⑦ Immersionoil
⑧ Tensionadjustmenttool
⑨ Dustcover
⑩ Microscopepowersupply
⑪ Lightexcludingplate
⑫ HBOmercurybulb
①
③
④
⑤⑥
⑦
⑧
②
⑨
⑩
⑫⑪
① Frame
② Objectives
③ Trinocularobservationhead
④ LEDuorescenceilluminator
⑤ Eyepieces
⑥ Dustcover
⑦ Immersionoil
⑧ Tensionadjustmenttool
⑨ Allenwrench
⑩ Lightexcludingplate
⑪ Powersupply
①
③
④
⑤⑥
⑦
⑧
②
⑨⑩
⑪

Page 7
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 8
EYEPIECES
PHOTO/TV PORT
(NOT USABLE WITH
ALC MODELS)
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY DIAL
CONDENSER
STAGE
CONDENSER HEIGHT
ADJUSTMENT KNOB
NOSEPIECE
OBJECTIVES
ALC SYSTEM
CONNECTION
CABLE
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
FINE FOCUS KNOB
COARSE FOCUS
KNOB
ALC SYSTEM
ON /OFF BUTTON
SLIDE HOLDER
TENSION
ADJUSTMENT
RING
7. Instrument description
7.1 B-382PL-ALC / B-382PLI-ALC

Page 9
EYEPIECES
PHOTO/TV PORT
OBSERVATION
HEAD
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY DIAL
CONDENSER
STAGE
NOSEPIECE
OBJECTIVES
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
FINE FOCUS KNOB
COARSE FOCUS
KNOB
SLIDE HOLDER
DIOPTER
ADJUSTMENT
RING
TENSION
ADJUSTMENT
RING
7.2 B-383PL / B-383PLI
CONDENSER HEIGHT
ADJUSTMENT KNOB

Page 10
EYEPIECES
PHOTO/TV PORT
(NOT USABLE WITH
ALC MODELS)
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
NOSEPIECE
OBJECTIVES
ALC SYSTEM
CONNECTION
CABLE
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
ALC SYSTEM
ON /OFF BUTTON
SLIDE HOLDER
TENSION
ADJUSTMENT
RING
COARSE FOCUS
KNOB
FINE FOCUS KNOB
CONDENSER HEIGHT
ADJUSTMENT KNOB
7.3 B-382PH-ALC / B-382PHI-ALC