Optika Italy B-380 Series User manual

Model
B-382PL-ALC
B-383PL
B-382PLI-ALC
B-383PLI
B-382PH-ALC
B-383PH
B-382PHI-ALC
B-383PHI
B-383FL
B-383LD
B-380 Series
INSTRUCTION MANUAL
Ver. 6.4 2022

Page 2
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 B-382PL-ALC / B-382PLI-ALC 4
3.2 B-383PL / B-383PLI 4
3.3 B-382PH-ALC / B-382PHI-ALC 5
3.4 B-383PH / B-383PHI 5
3.5 B-383FL 6
3.6 B-383LD 6
4. Unpacking 7
5. Intended use 7
6. Symbols and conventions 7
7. Instrument description 8
7.1 B-382PL-ALC / B-382PLI-ALC 8
7.2 B-383PL / B-383PLI 9
7.3 B-382PH-ALC / B-382PHI-ALC 10
7.4 B-383PH / B-383PHI 11
7.5 B-383FL 12
7.6 B-383LD 14
8. Assembling 15
8.1 Assembling the microscope 15
8.2 Field Diaphragm (Optional) 16
8.3 Polarizing set (optional) 16
9. Brightfield transmitted light observation procedures 18
10. Use of the microscope (Brightfield transmitted light) 19
10.1 Light intensity adjustment 19
10.2 Adjust the interpupillary distance 19
10.3 Diopter adjustment 19
10.4 Coarse focus tension adjustment 19
10.5 Focus lock lever 20
10.6 Stage 20
10.7 Condenser centering 20
10.7.1 Centeringwithoutelddiaphragm 20
10.7.2 Centeringwithelddiaphragm 21
10.8 E󰀨ectsoftheelddiaphragm 21
10.9 Aperture diaphragm 21
10.10 Use of oil immersion objective 22
10.11 Use of ALC system 22
10.12 Use of the polarizer (optional) 22
11. Use of universal condenser for Brightfield/Darkfield/Phase Contrast 23
11.1 Brighteldobservation(BF) 23
11.2 Darkeldobservation(DF) 23
11.3 Phase contrast observation (PH) 24
11.4 Useofthegreenlter 25
12. Fluorescence reflected light observation procedures (B-383FL) 26
13. Fluorescence reflected light observation procedures (B-383LD) 26
14. Use of the microscope (Fluorescence reflected light) 27
14.1 Assembling procedure (B-383FL) 27
14.2 Assembling procedure (B-383LD) 27
14.3 HBO bulb assembling (B-383FL) 28
14.4 Centering the HBO bulb (B-383FL) 30
14.5 Use of the microscope (B-383FL) 32
14.6 Use of the microscope (B-383LD) 32
14.7 Use of the shutter 33
14.8 Use of the light excluding plate 33
15. Simultaneous observation Phase Contrast + Fluorescence (B-383FL) 34
16. Microphotography 35
16.1 Installing the C-mount adapter 35
16.2 Useofreexcameras 35
17. Maintenance 36
18. Troubleshooting 37
Equipment disposal 39
Table of content

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4
3. Package content
3.1 B-382PL-ALC / B-382PLI-ALC
3.2 B-383PL / B-383PLI
① Frame
② Objectives
③ ALCobservationhead
④ Eyepieces
⑤ Tensionadjustmenttool
⑥ Dustcover
⑦ Powersupply
⑧ Immersionoil
①
③
④
⑤
⑥
⑦
⑧
②
① Frame
② Objectives
③ Trinocularobservationhead
④ Eyepieces
⑤ Tensionadjustmenttool
⑥ Dustcover
⑦ Powersupply
⑧ Immersionoil
①
③
④
⑤
⑥⑦
⑧
②

Page 5
3.3 B-382PH-ALC / B-382PHI-ALC
3.4 B-383PH / B-383PHI
① Frame
② Objectives
③ ALCobservationhead
④ Eyepieces
⑤ Tensionadjustmenttool
⑥ Dustcover
⑦ Powersupply
⑧ Immersionoil
⑨ Greenlter+lterholder
⑩ Centeringtelescope
①
③
④
⑤
⑥⑦
⑧
②
⑨
⑩
① Frame
② Objectives
③ Trinocularobservationhead
④ Eyepieces
⑤ Tensionadjustmenttool
⑥ Dustcover
⑦ Powersupply
⑧ Immersionoil
⑨ Greenlter+lterholder
⑩ Centeringtelescope
①
③
④
⑤
⑥⑦
⑧
②
⑨
⑩

Page 6
3.5 B-383FL
3.6 B-383LD
① Frame
② Objectives
③ Trinocularobservationhead
④ HBOuorescenceilluminator
⑤ Fluorescencepowersupply+powercord
⑥ Eyepieces
⑦ Immersionoil
⑧ Tensionadjustmenttool
⑨ Dustcover
⑩ Microscopepowersupply
⑪ Lightexcludingplate
⑫ HBOmercurybulb
①
③
④
⑤⑥
⑦
⑧
②
⑨
⑩
⑫⑪
① Frame
② Objectives
③ Trinocularobservationhead
④ LEDuorescenceilluminator
⑤ Eyepieces
⑥ Dustcover
⑦ Immersionoil
⑧ Tensionadjustmenttool
⑨ Allenwrench
⑩ Lightexcludingplate
⑪ Powersupply
①
③
④
⑤⑥
⑦
⑧
②
⑨⑩
⑪

Page 7
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 8
EYEPIECES
PHOTO/TV PORT
(NOT USABLE WITH
ALC MODELS)
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY DIAL
CONDENSER
STAGE
CONDENSER HEIGHT
ADJUSTMENT KNOB
NOSEPIECE
OBJECTIVES
ALC SYSTEM
CONNECTION
CABLE
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
FINE FOCUS KNOB
COARSE FOCUS
KNOB
ALC SYSTEM
ON /OFF BUTTON
SLIDE HOLDER
TENSION
ADJUSTMENT
RING
7. Instrument description
7.1 B-382PL-ALC / B-382PLI-ALC

Page 9
EYEPIECES
PHOTO/TV PORT
OBSERVATION
HEAD
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY DIAL
CONDENSER
STAGE
NOSEPIECE
OBJECTIVES
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
FINE FOCUS KNOB
COARSE FOCUS
KNOB
SLIDE HOLDER
DIOPTER
ADJUSTMENT
RING
TENSION
ADJUSTMENT
RING
7.2 B-383PL / B-383PLI
CONDENSER HEIGHT
ADJUSTMENT KNOB

Page 10
EYEPIECES
PHOTO/TV PORT
(NOT USABLE WITH
ALC MODELS)
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
NOSEPIECE
OBJECTIVES
ALC SYSTEM
CONNECTION
CABLE
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
ALC SYSTEM
ON /OFF BUTTON
SLIDE HOLDER
TENSION
ADJUSTMENT
RING
COARSE FOCUS
KNOB
FINE FOCUS KNOB
CONDENSER HEIGHT
ADJUSTMENT KNOB
7.3 B-382PH-ALC / B-382PHI-ALC

Page 11
EYEPIECES
PHOTO/TV PORT
OBSERVATION
HEAD
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
CONDENSER
STAGE
CONDENSER HEIGHT
ADJUSTMENT KNOB
NOSEPIECE
OBJECTIVES
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
SLIDE HOLDER
DIOPTER
ADJUSTMENT
RING
TENSION
ADJUSTMENT
RING
COARSE FOCUS
KNOB
FINE FOCUS KNOB
7.4 B-383PH / B-383PHI

Page 12
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
FOCUS LOCK
LEVER
MAIN SWITCH /
LIGHT INTENSITY
DIAL
STAGE
CONDENSER HEIGHT
ADJUSTMENT KNOB
NOSEPIECE
OBJECTIVES
SLIDE HOLDER
EYEPIECES
HBO FLUORESCENCE
EPI-ILLUMINATOR
COARSE FOCUS
KNOB
FINE FOCUS KNOB
PHOTO/TV PORT
7.5 B-383FL

Page 13
CONDENSER
LAMP
HOUSING
X-Y
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
TENSION
ADJUSTMENT
RING
HBO FLUORESCENCE
POWER SUPPLY
FLUORESCENCE
FILTER SLIDER
B-383FL (Opposite side)

Page 14
FOCUS LOCK
LEVER
LIGHT INTENSITY
DIAL
STAGE
CONDENSER HEIGHT
ADJUSTMENT KNOB
NOSEPIECE
OBJECTIVES
SLIDE HOLDER
LED FLUORESCENCE
EPI-ILLUMINATOR
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
EYEPIECES
PHOTO/TV PORT
X-Y
MOVEMENT
KNOBS
CONDENSER
CONDENSER
CENTERING
SCREWS
FLUORESCENCE
FILTER SLIDER
FLUORESCENCE
LED
TENSION
ADJUSTMENT
RING
FINE FOCUS KNOB
COARSE FOCUS
KNOB
7.6 B-383LD

Page 15
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
FFig. 4ig. 4
8. Assembling
8.1 Assembling the microscope
1. Insert the optical head above the stand and tight-
en the screw with the provided Allen wrench. (Fig. 1)
ALC models only
2. Connect the ALC cable to the socket on the back of the
frame. (Fig. 2)
3. Insert eyepieces into the empty tubes of the optical head.
(Fig. 3)
4. Screw each objective into the thread of the nosepiece,
clockwise with increasing magnication. (Fig. 4)

Page 16
FFig. 7ig. 7
①
FFig. 8ig. 8
②
FFig. 5ig. 5
FFig. 6ig. 6
5. Insert the power supply jack in the socket placed in the rear
side of the frame. (Fig. 5)
8.2 Field Diaphragm (Optional)
1. Unscrew the lens at the base of the microscope. (Fig. 6)
• It may take a little bit of force to unscrew the lens.
2. Fully screw the eld diaphragm (M-156).
3. System is ready for the use.
8.3 Polarizing set (optional)
1. Place the polarizer on the light exit ① at the base of the mi-
croscope. (Fig. 7)
2. Loosen the head xing knob ② and remove the head from
the microscope frame. (Fig. 8)

Page 17
FFig. 9ig. 9
③
3. Insert the analyzer into the hole inside the frame ③. (Fig. 9)
4. Put back the head into its original position and lock the xing
knob.
• The use of the polarization set, although possible for
models B-383FL and B-383LD, is not recommended. The
presence of the analyzer within the optical path, during
theuseofuorescence,causesasignicantreduction
in the amount of light projected on the sample, resulting
indi󰀩cultyofobservation.

Page 18
9. Brightfield transmitted light observation procedures
Main switch / brightness adjustment knob
(Used commands) (Chapter)
Nosepiece
Observation head
Diopter adjustment ring
Condenser centering
Aperture diaphragm
Field diaphragm
Nosepiece
Main switch / brightness adjustment knob
Coarse and fine focusing knobs
Coarse and fine focusing knobs
Set the main switch to “ON” and adjust light intensity.
Insert 10X objective into the light path
Focus the specimen
Begin observation
Adjust field and aperture iris diaphragms
Insert desired objective for the observation and focus the
specimen
Adjust interpupillary distance
Adjust diopters
Center optical axis
Adjust light intensity
10
7
7
7
7
10
10
10
10
10
10
Slide holder
Place a specimen on the stage. 10

Page 19
FFig. 10ig. 10
①
FFig. 12ig. 12
②
FFig. 13ig. 13
③
10. Use of the microscope (Brightfield transmitted light)
10.1 Light intensity adjustment
Operate on the light intensity dial ①to turn ON/OFF the micro-
scope and to increase or decrease the illumination intensity. (Fig.
10)
• Only for B-383LD: the switch located at the back of the
microscope operates to turn on the transmitted light (po-
sition“I”)orthereectedlight(position“II”).Turnonthe
microscope for transmitted light by turning the switch
to “I”.
FFig. 11ig. 11
②
10.2 Adjust the interpupillary distance
Hold the right and left parts of the observation head using both
hands and adjust the interpupillary distance by turning the two
parts until one circle of light can be seen. (Fig. 11)
10.3 Diopter adjustment
1. Look into the right eyepiece with your right eye only, and fo-
cus on the specimen.
2. Look into the left eyepiece with your left eye only. If the image
is not sharp, use the diopter adjustment ring ②to compen-
sate. (Fig. 12)
• Highpoint eyepieces allow the use also to glass wearers.
• NOTE: For optimal parfocality, we recommend using your
glasses during normal microscope use.
10.4 Coarse focus tension adjustment
To adjust the tension according to personal’s needs, rotate the
ring ③ using the provided tool (Fig. 13). Clockwise rotation in-
creases the tension.
• NOTE: If the tension is too loose, the stage could go low-
erbyitselforthefocuseasilylostafterneadjustment.
In this case, rotate the knob in order to increase the ten-
sion.

Page 20
FFig. 15ig. 15
②
FFig. 16ig. 16
⑤
③
④
10.5 Focus lock lever
The upper limit knob has two functions: prevent the contact
between slide and objective and acts as focus memory.
1. After focusing the specimen, rotate the knob ① and lock it
(Fig. 14). In this way the focus upper limit is set.
2. Lower the stage with coarse focus knob and replace the
specimen.
3. Raise again the stage up to the upper limit: specimen will be
in approximate focus and will need a ne adjustment to get
the proper focus. Fine focus movement is not a󰀨ected by the
coarse focus lock.
• To unlock, move the knob in the opposite direction to the
one used for the lock. FFig. 14ig. 14
LOCK
UNLOCK
①
10.6 Stage
Stage accepts standard slides 26 x 76 mm, thickness 1,2 mm
with coverslide 0,17mm. (Fig. 15)
It is possible to place two slides side by side on the stage.
1. Open the spring arm of the slide holder ② and place frontally
the slides on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the fall-
ing of the slide.
10.7 Condenser centering
10.7.1 Centeringwithoutelddiaphragm
The condenser is installed and pre-centered in the factory.
To remove the condenser use an Allen wrench 1.5 mm and oper-
ate on the xing knob placed on the right side of the condenser
holder.
Should a new centering is needed, operate in this way:
1. Insert 4x objective in the light path (in case 4x is not available
use the lower magnication available).
2. Focus the specimen.
3. Close the aperture diaphragm using the ring ③, moving the
ring to the value “4” related to the 4x objective. (Fig. 16)
4. Raise the condenser to the upper limit using the height ad-
justment knob ④ placed on the left side of the condenser
holder.
5. Center the condenser using the centering screws ⑤ until the
eld of view is evenly illuminated (in the eld of view no dark
and bright areas must be noticed).
6. Fully open the diaphragm.