Optika Italy B-1000FL-HBO User manual

Model
B-1000FL HBO
B-1000 Series
INSTRUCTION MANUAL
Ver. 4.0 2019

Page 2
1. Warning 4
2. Symbols and conventions 4
3. Safety Information 4
4. Intended use 4
5. Overview 5
5.1 Manual version 5
5.2 Motorised version 7
6. Unpacking 8
7. Assembling 8
7.1 Manual version 8
7.2 Motorised version 9
7.3 Assembling the microscope 10
7.4 Only for motorised version 14
8. Brighteldobservationprocedures(transmittedlight) 15
9. UseofthemicroscopeinBrighteld(transmittedlight) 16
9.1 General switch on 16
9.2 Control keyboard 16
9.3 Brightness adjustment 16
9.4 Adjust the observation head 17
9.5 Adjust the interpupillary distance 17
9.6 Diopter adjustment 17
9.7 Use of eyeshields 18
9.8 Light path selection 18
9.9 Coarse focus tension adjustment 18
9.10 Focus stop lever 19
9.11 Stage 19
9.12 Condenser centering 20
9.13 Effectofelddiaphragm 20
9.14 Aperture diaphragm 20
9.14 Use of oil immersion objective 21
9.15 Only for motorised version 21
9.15.1 Nosepiece rotation 21
9.15.2 Focusing 22
9.15.3 Stage 22
10. Fluorescence observation procedures 23
11. Use of the microscope in Fluorescence 24
11.1 HBO bulb switch on 24
11.2 Centering of HBO bulb 24
11.3 Use of diaphragms 26
11.3.1 Field diaphragm 26
11.3.2 Aperture diaphragm 26
11.4 Use of the shutter 26
11.5 Useofuorescence 27
11.6 Use of light excluding plate 27
12. UseofcondenserforBrighteld/Darkeld/PhaseContrast(optional) 28
12.1 Brighteldobservation(BF) 28
12.2 Darkeldobservation(DF) 28
12.3 Phasecontrastobservation(PH) 29
12.4 Useofgreenlter 30
13. SimultaneousobservationinPhaseContrast+Fluorescence 30
14. DifferentialInterferenceContrastDICObservation(optional) 31
14.1 Koehler DIC transmitted light 31
14.2 Nomarski DIC transmitted light 32
15. Simultaneous Observation in Fluorescence and DIC 34
15.1 KoehlerDICreectedlight 34
15.2 NomarskiDICreectedlight 36
16. Microphotography 39
16.1 Use of “C”mount camera 39
16.2UseofReexcamera 39
Table of Contents

Page 3
17. Maintenance 40
18. Troubleshooting 41
Equipment disposal 43

Page 4
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use that
does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 5
5. Overview
EYEPIECE
PHOTO / TV
PORT
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
CONTROL
KEYBOARD
MAIN SWITCH
FOCUS LOCK
LEVER
INTENSITY
ADJUSTMENT
DIAL
FIELD
DIAPHRAGM
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
FLUORESCENCE
POWER SUPPLY
UV SHIELD
5.1 Manual version