Optika B-1000 Series User manual

Model
B-1000
B-1000BF
B-1000PH
B-1000TI-2
B-1000TI-3
B-1000TI-5
B-1000TI-10
B-1000 Series
INSTRUCTION MANUAL
Ver. 3.2 2020

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Instrument description 4
5.1 Manual version 4
5.2 Motorized version 6
5.3 B-1000TI-2 / 3 / 5 / 10 8
6. Unpacking 9
7. Assembling 9
7.1 Manual version 9
7.2 Motorized version 10
7.3 B-1000TI-2/3/5/10 11
7.4 Assembling the microscope 12
7.4.1 Manual version 12
7.4.2 Motorized version 14
7.4.3 B-1000TI-2 / 3 / 5 / 10 15
8. SummaryofBrighteldobservationprocedures 18
9. Use of the microscope 19
9.1 General switch on 19
9.2 Control keyboard 19
9.3 Brightness adjustment 19
9.4 Adjust the observation head 20
9.5 Adjust the interpupillary distance 20
9.6 Diopter adjustment 20
9.7 Use of eyeshields 20
9.8 Light path selection 21
9.9 Coarse focus tension adjustment 21
9.10 Focus stop lever 22
9.11 Stage 22
9.12 Centering the condenser 23
9.13 Effectofelddiaphragm 23
9.14 Aperture diaphragm 23
9.15 Use of oil immersion objective 24
9.16 Use of ALC system (optional) 24
9.17 Only for motorized version 25
9.17.1 Nosepiece rotation 25
9.17.2 Focusing 25
9.17.3 Stage 25
9.18 Use of the pointer (B-1000TI-2 / 3 / 5 / 10) 26
10. UseofuniversalcondenserforBrighteld/Darkeld/Phasecontrast 27
10.1 Brighteldobservation(BF) 27
10.2 Darkeldobservation(DF) 27
10.3 PhaseContrastobservation(PH) 28
10.4 Useofgreenlter 29
11. DIC observation 30
11.1 Koehler DIC transmitted light 30
11.2 Nomarski DIC transmitted light 31
12. Microphotography 33
12.1 Use of C-mount cameras 33
12.2 Useofreexcamera 33
13. Maintenance 34
14. Troubleshooting 35
Equipment disposal 37
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICALSHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
EYEPIECE
PHOTO / TV
PORT
OBSERVATION
HEAD
ALC SYSTEM
DIOPTER
ADJUSTMENT
RING
DIC PRISM
SLOT
CONTROL
KEYBOARD
MAIN
SWITCH
FOCUS LOCK
LEVER INTENSITY
ADJUSTMENT
DIAL
FIELD
DIAPHRAGM
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
5. Instrument description
5.1 Manual version

Page 5
FILTER
HOLDER
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
TENSION
ADJUSTMENT
RING
APERTURE
DIAPHRAGM
LIGHT PATH
SELECTOR
LEVER
SLIDE
HOLDER
X-Y STAGE
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
ALC SOCKET
Opposite side

Page 6
NOSEPIECE
FOCUS
MOTOR
X-AXIS MOVEMENT
KNOB
(MANUAL MOVEMENT)
STAGE
5.2 Motorized version
Only the parts related to the motorized version are highlighted.

Page 7
STAGE
CONNECTION
CABLE
NOSEPIECE
ROTATION
BUTTONS
Y-AXIS MOVEMENT
KNOB
(MANUAL MOVEMENT)
Opposite side

Page 8
POINTER BRIGHTNESS
ADJUSTMENT / POINTER
MOVEMENT
SIDE
OBSERVATION
HEADS
POINTER COLOR
SELECTOR
5.3 B-1000TI-2 / 3 / 5 / 10
Only the multi-head parts are highlighted.

Page 9
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) (Optional)
⑧ Power supply
⑨ Dust cover
⑩ Allen wrench
⑪ Immersion oil
③
④
⑤
⑥
⑦
⑧
①
②
⑪
⑩
⑨
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 Manual version

Page 10
⑪⑩
⑨
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) (Optional)
⑧ Microscope power supply
⑨ Motorized parts power supply
⑩ Serial cable
⑪ PS/2 mouse
⑫ Dust cover
⑬ Allen wrench
⑭ Immersion oil
①②
③
④
⑤
⑥
⑦
⑧
⑫⑬⑭
7.2 Motorized version