Optika B-1000 Series User manual

Model
B-1000
B-1000BF
B-1000PH
B-1000TI-2
B-1000TI-3
B-1000TI-5
B-1000TI-10
B-1000 Series
INSTRUCTION MANUAL
Ver. 3.2 2020

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Instrument description 4
5.1 Manual version 4
5.2 Motorized version 6
5.3 B-1000TI-2 / 3 / 5 / 10 8
6. Unpacking 9
7. Assembling 9
7.1 Manual version 9
7.2 Motorized version 10
7.3 B-1000TI-2/3/5/10 11
7.4 Assembling the microscope 12
7.4.1 Manual version 12
7.4.2 Motorized version 14
7.4.3 B-1000TI-2 / 3 / 5 / 10 15
8. SummaryofBrighteldobservationprocedures 18
9. Use of the microscope 19
9.1 General switch on 19
9.2 Control keyboard 19
9.3 Brightness adjustment 19
9.4 Adjust the observation head 20
9.5 Adjust the interpupillary distance 20
9.6 Diopter adjustment 20
9.7 Use of eyeshields 20
9.8 Light path selection 21
9.9 Coarse focus tension adjustment 21
9.10 Focus stop lever 22
9.11 Stage 22
9.12 Centering the condenser 23
9.13 Effectofelddiaphragm 23
9.14 Aperture diaphragm 23
9.15 Use of oil immersion objective 24
9.16 Use of ALC system (optional) 24
9.17 Only for motorized version 25
9.17.1 Nosepiece rotation 25
9.17.2 Focusing 25
9.17.3 Stage 25
9.18 Use of the pointer (B-1000TI-2 / 3 / 5 / 10) 26
10. UseofuniversalcondenserforBrighteld/Darkeld/Phasecontrast 27
10.1 Brighteldobservation(BF) 27
10.2 Darkeldobservation(DF) 27
10.3 PhaseContrastobservation(PH) 28
10.4 Useofgreenlter 29
11. DIC observation 30
11.1 Koehler DIC transmitted light 30
11.2 Nomarski DIC transmitted light 31
12. Microphotography 33
12.1 Use of C-mount cameras 33
12.2 Useofreexcamera 33
13. Maintenance 34
14. Troubleshooting 35
Equipment disposal 37
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICALSHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
EYEPIECE
PHOTO / TV
PORT
OBSERVATION
HEAD
ALC SYSTEM
DIOPTER
ADJUSTMENT
RING
DIC PRISM
SLOT
CONTROL
KEYBOARD
MAIN
SWITCH
FOCUS LOCK
LEVER INTENSITY
ADJUSTMENT
DIAL
FIELD
DIAPHRAGM
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES
5. Instrument description
5.1 Manual version

Page 5
FILTER
HOLDER
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
TENSION
ADJUSTMENT
RING
APERTURE
DIAPHRAGM
LIGHT PATH
SELECTOR
LEVER
SLIDE
HOLDER
X-Y STAGE
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
ALC SOCKET
Opposite side

Page 6
NOSEPIECE
FOCUS
MOTOR
X-AXIS MOVEMENT
KNOB
(MANUAL MOVEMENT)
STAGE
5.2 Motorized version
Only the parts related to the motorized version are highlighted.

Page 7
STAGE
CONNECTION
CABLE
NOSEPIECE
ROTATION
BUTTONS
Y-AXIS MOVEMENT
KNOB
(MANUAL MOVEMENT)
Opposite side

Page 8
POINTER BRIGHTNESS
ADJUSTMENT / POINTER
MOVEMENT
SIDE
OBSERVATION
HEADS
POINTER COLOR
SELECTOR
5.3 B-1000TI-2 / 3 / 5 / 10
Only the multi-head parts are highlighted.

Page 9
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) (Optional)
⑧ Power supply
⑨ Dust cover
⑩ Allen wrench
⑪ Immersion oil
③
④
⑤
⑥
⑦
⑧
①
②
⑪
⑩
⑨
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 Manual version

Page 10
⑪⑩
⑨
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) (Optional)
⑧ Microscope power supply
⑨ Motorized parts power supply
⑩ Serial cable
⑪ PS/2 mouse
⑫ Dust cover
⑬ Allen wrench
⑭ Immersion oil
①②
③
④
⑤
⑥
⑦
⑧
⑫⑬⑭
7.2 Motorized version

Page 11
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Main observation head
⑥ Side observation heads
• one for B-1000TI-2
• two for B-1000TI-3
• four for B-1000TI-5
• nine for B-1000TI-10
⑦ Eyepieces
• 10x/22 (one pair for main head)
• 10x/20 (one pair for B-1000TI-2)
• 10x/20 (two pairs for B-1000TI-3)
• 10x/20 (four pairs for B-1000TI-5)
• 10x/20 (nine pairs for B-1000TI-10)
⑧ Power supply
• one for microscope (6V dc)
• one for multi-head attachment (5Vdc)
⑨ Dust cover
⑩ Allen wrench
⑪ Immersion oil
①
②
③
④
⑤
⑥
⑦
⑪
⑨⑩⑧
7.3 B-1000TI-2/3/5/10

Page 12
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
7.4 Assembling the microscope
7.4.1 Manual version
1. Put the microscope stand on a solid table. Insert
M-1030 attachment (if provided) above the stand, using
the 2mm Allen wrench to tighten the screw. (Fig. 1)
2. Connect the cable of the ALC (Automatic Light Con-
trol) system to the socket placed on the right side of the
frame. (Fig. 2)
3. Insert the optical head above the attachment, using the
2mm Allen wrench to tighten the screw. (Fig. 3)
4. Insert eyepieces into the empty eyepiece sleeves. (Fig.
4)

Page 13
①
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 8ig. 8
FFig. 7ig. 7
②
5. Insert the condenser under the stage: position until it is
well inserted into its holder (under the condenser there
is a pin that must fully enter the holder guide). (Fig. 5)
6. Lock the condenser xing knob ①.
7. Mount the stage: lower the support using the coarse
focus knob, then place the stage and rmly tighten the
lock screw ②. (Fig. 6)
8. Screw each objective into the thread of the nosepiece,
clockwise with increasing magnication. (Fig. 7)
9. Insert the power supply jack in the socket placed in
the rear side of the of the microscope. (Fig. 8)

Page 14
FFig. 9ig. 9
FFig. 10ig. 10
FFig. 11ig. 11
②
①
③ ④ ⑤⑥
7.4.2 Motorized version
1. Assemble the stage in the same way as the manual
version. Check the perfect alignment of the rear part of
the stage with the rear arm of the frame. An imperfect
alignment could lead to an incorrect functioning of the
system. (Fig. 9)
2. Connect the cable ① from the stage to the frame and
tight the locking screws of the connectors ②. (Fig. 10)
3. Connect the provided cables: ③ 12V power supply for
the motorized parts; ④ 6V microscope power supply;
⑤ serial cable; ⑥ PS/2 mouse. Connect power cables
as the last step. (Fig. 11)

Page 15
①
FFig. 12ig. 12
FFig. 13ig. 13
FFig. 15ig. 15
FFig. 14ig. 14
①
7.4.3 B-1000TI-2 / 3 / 5 / 10
1. Place the splitter attachment of the multi-discussion
system and tighten the lock screw ① on the right side
of the frame. (Fig. 12)
2. Connect the 5Vdc power supply to the rear socket of
the splitter attachment. (Fig. 13).
3. Connect the rst part of the extension tube to the optical
splitter. Insert the tube into the splitter all the way down
and screw on the black sealing ring completely. (Fig.
14-15).
• Each connection is labeled with a letter printed
on both sides of the connection. Make sure to
match the letters in order to correctly assemble the
microscope.

Page 16
FFig. 16ig. 16
①
②
③
FFig. 17ig. 17
FFig. 19ig. 19
FFig. 18ig. 18
4. Insert the second part of the extension tube. (Fig. 16).
5. Fully insert the second extension tube in the right
position. Using the provided Allen wrench (small one)
lock the xing screws ① to block the extension tube.
• At the end of the rst extension tube there is a
lens (Fig. 17). Make sure it is free from dirt, dust
or other contaminants before to proceed with the
assemblingofthesecondextensiontube.
6. Adjust the height of the multi-head holder. Loosen
the base xing knob ②, unscrew the base ③ in order
to reach the desired height, then lock the knob. (Fig.
18). Make sure that each extension tube is perfectly
horizontal.
7. Insert the binocular heads, matching the reference
letter. (Fig. 19).

Page 17
FFig. 20ig. 20
FFig. 21ig. 21
8. Insert the provided eyepieces (WF10X/20) into binocular
heads. (Fig. 20)
9. Repeat all the above operations for each observation
point.
10. Install the trinocular head over the splitter. (Fig. 21)
11. Continue with the installation of all other components
as described in the paragraph 7.4.1.

Page 18
Main switch
(Used commands) (Chapter)
Slide holder
Nosepiece
Observation head
Diopter adjustment ring
Condenser centering
Aperture diaphragm
Field diaphragm
Nosepiece
Light adjustment dial
Coarse and fine focusing knobs
Coarse and fine focusing knobs
Bring on “1” the main switch
Put a specimen on the stage
Insert 10X objective into the light path
Focus the specimen
Begin observation
Adjust field and aperture iris diaphragms
Insert desired objective for the observation and focus the
specimen
Adjust interpupillary distance
Adjust diopters
Center optical axis
Adjust light intensity
9
9
5
5
5
5
9
9
9
9
9
9
ON-OFF key on the keyboard
Light adjustment dial
Turn on the microscope and adjust light intensity 9
9
ALC system 9
8. SummaryofBrighteldobservationprocedures

Page 19
③
④
②
FFig. 23ig. 23
FFig. 24ig. 24
⑤
①
FFig. 22ig. 22
①
9. Use of the microscope
9.1 General switch on
To activate the transmitted light illuminator, put the main
switch ①, located on the left side of the stand, to the posi-
tion “1”. (Fig. 22)
9.2 Control keyboard
B-1000 illumination can be managed through the keyboard
placed on the left of the stand. (Fig. 23)
• ON-OFF (②): press this key (after switching the main
switch on “1”) to turn ON or OFF the microscope LED.
• BOOST (③): press this button in order to in-
crease the brightness (useful for high-magni-
cation objectives or very opaque specimens).
Do not enable boost mode while observing with low
magnication objectives (4x, 10x) with fully open
diaphragm: the high brightness may hurt user’s
eyes.
• AUTO OFF (④): if you want the illuminator to switch off
automatically, press this button until 15, 30 or 60 min-
utes delay is set. After this period of time, the light will
turn off. You have to press the ON-OFF button to turn
it on again.
9.3 Brightness adjustment
Use the brightness adjustment dial ⑤ on the left side of the
microscope to increase or decrease the light intensity on
the specimen. (Fig. 24)

Page 20
FFig. 25ig. 25
FFig. 26ig. 26
FFig. 28ig. 28
FFig. 27ig. 27
①
③
②
9.4 Adjust the observation head
Loosen the locking screw ①, turn the observation head to
a comfortable position for observation, and then lock the
locking screw again. (Fig. 25)
9.5 Adjust the interpupillary distance
Observing with both eyes, hold the two eyepiece prism as-
semblies. Rotate them around their common axis until the
elds of view coincide.
• The graduation on the interpupillary distance
indicator ②, pointed by the spot “.” on the eyepiece
holder, shows the distance between the operator’s
eyes. (Fig. 26)
The range of the interpupillary distance is 48-75 mm.
9.6 Diopter adjustment
1. Look into the right eyepiece with your right eye only,
and focus on the specimen.
2. Look into the left eyepiece with your left eye only. If the
image is not sharp, use the diopter adjustment ring ③
to compensate. (Fig. 27)
• The adjustment range is ±5 diopter. The number
indicated on the adjustment ring graduation should
correspond to the operator’s diopter correction.
9.7 Use of eyeshields
• Use with eyeglasses
Fold rubber eyeshields with both hands. Folded eyeshields
avoid scratching the lenses of eyeglasses. (Fig. 28)