Optika B-500TiFL User manual

OPTIKA MICROSCOPES - ITALY
www.optikamicroscopes.com - [email protected]
Ver. 1.0.0
B-500TiFL
OPERATION MANUAL
GUIDA UTENTE
MANUAL DE INSTRUCCIONES
MANUEL D’INSTRUCTIONS

Page 2
INDEX
1.0 DESCRIPTION page 4
2.0 INTRODUCTION page 6
3.0 UNPACKING AND ASSEMBLY page 6
4.0 USING THE MICROSCOPE page 8
5.0 MAINTENANCE page 12
6.0 ELECTRICAL SPECIFICATIONS page 13
7.0 RECYCLING AND RECOVERY page 14

Page 3
This microscope is a scientic precision instrument designed to last for many years with a minimum of main-
tenance. It is built to high optical and mechanical standards and to withstand daily use.
Optika reminds you that this manual contains important information on safety and maintenance, and that it
must therefore be made accessible to the instrument users.
Optika declines any responsibility deriving from instrument uses that do not comply with this ma-nual.
Safety guidelines
This manual contains important information and warnings regarding safety about installation, use and
maintenance of the microscope. Please read this manual carefully before using the equipment. To
ensure safe use, the user must read and follow all instructions in this manual. OPTIKA products are
designed for safe use in normal operating conditions. The equipment and accessories described in
the manual are manufactured and tested according to industry standards for safety instrumentation
laboratory. Misuse can cause personal injury or damage to the instrument. Keep this manual at hand
close to the instrument, for an easy consultation.
Electrical safety
Before connecting the power cord to wall outlet, ensure that your mains voltage for your region corre-
sponds to the voltage supply of the instrument, and that the illuminator’s switch is in position OFF. The
user must observe the safety regulations in force in his region. The instrument is equipped with CE
safety marking, in any case the user has full responsibility concerning the safe use of that instrument.
Warning/Caution symbols used in this manual
The user should be aware of safety aspects when using the instrument. Warning or hazard symbols
are shown below. These symbols are used in this manual.
The instructions on this symbol to avoid possible severe personal injuries.
Warning of use; the incorrect operation on the instrument can cause damages
to the person or instrument.
Possibility of electric shock.
Attention: high temperature surfaces. Avoid direct contact.
Technical notes or usage tips.
SAFETY GUIDELINES

Page 4
1.0 DESCRIPTION
POWER
SUPPLY
FILTERS BLOCK
LAMP CENTERING
KNOBS
HG LAMP HOUSING
PHOTO PORT
OBSERVATION
HEAD
EYEPIECE
DIOPTRIC
ADJUSTMENT
RING
NOSEPIECE
OBJECTIVE
STAGE WITH
CLAMP
APERTURE
DIAPHRAGM
(BRIGHTFIELD)
FIELD DIAPHRAGM
(BRIGHTFIELD)
COARSE FOCUSING
KNOB
FINE FOCUSING KNOB

Page 5
1.0 DESCRIPTION
SPECIFIC PARTS FOR BRIGHTFIELD ILLUMINATION:
POWER
SUPPLY
CONDENSER
CONDENSER-CENTRING
SCREWS
TRANSLATION KNOBS
CONDENSER
ADJUSTMENT
KNOB (HEIGHT)
LIGHT PATH SELECTOR LEVER
PHOTO PORT

Page 6
LAMP SCREW
This microscope is a scientic precision instrument designed to last for many years with a minimum of main-
tenance. It is built to high optical and mechanical standards and to withstand daily classroom and laboratory
use.
Optika reminds you that this manual contains important information on safety and maintenance, and that it
must therefore be made accessible to the instrument users.
Optika declines any responsibility deriving from instrument uses that do not comply with this manual.
3.0 UNPACKING AND ASSEMBLY
3.1 The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of
the container and lift the top half of the container. Take some care to avoid that the optical items (ob-
jectives and eyepieces) fall out and get damaged. Using both hands (one around the arm and one
around the base), lift the microscope from the container and put it on a stable desk.
3.2 The objectives are located in individual protective vials. Remove the objectives from the vials and
insert them into the microscope nosepiece in the order from the lowest magnication to the highest,
in a clockwise direction from the rear.
3.3 Epiuorescence parts (epiilluminator with lters block, power supply and mercury lamp housing) are
housed in an hard case. Remove the protection caps from the top of the main B-600 body and from
the epiilluminator and place the epiilluminator on the top of the microscope body. Tighten the screw
on the right side using the provided allen wrench.
3.4 Insert the mercury lamp house on the back of the epiilluminator and tighten the screw using the pro-
vided allen wrench.
EPIILLUMINATOR
SCREW
2.0 INTRODUCION

Page 7
3.6 Insert the eyepieces into the eye tubes.
3.7 Fix the orange protection screen to the front of the epiilluminator with the two provided screws.
3.8 Mercury lamp insertion and replacement
Disconnect any cable from the epiilluminator. Loosen the lock-screw on the top of the lamp housing.
The lamp holder will come out with the door.
Remove the old lamp or the plastic placeholder. Use protection glasses as, if handled carelessly, there is a
risk that the lamp may shatter and eject glass splinters. Thus, without touching the glass part of the lamp, take
out the uorescence lamp from its box, taking care not to exert stress on any part of the lamp. Put one end
inside the exible metal holder (observe that it ts only in one direction) and then in the xed one. Tighten the
screws just enough to make it stay in place.If you tighten them too much the lamp may shatter. Put the lamp
door with lamp assembly back in place and connect the cable on the side of the epiilluminator to the power
supply. Connect the mains cable of the power supply. Turn on the power supply using the power switch. Wait
until the current display shows about 5.0 A. If the current falls below 4.5A, replace the lamp. You should now
wait for at least 10 minutes before aligning the lamp.
On the life time display you can read for how many hours the lamp has been in use. Replace the lamp every
500 hours, even if it seems to work correctly, as otherwise it may shatter. Take care to let the lamp cool down
at least 30 minutes before opening the lamp housing. After having replaced the lamp, press the reset button
to reset the life time count.
SCREW
LAMP
3.0 UNPACKING AND ASSEMBLY

Page 8
4.0 USING THE MICROSCOPE
Connect the mains plug into the socket at the base
Make sure, before you turn the illumination on, that the voltage selector is set to the mains voltage for
your region.
The power cord should be used only on network sockets equipped with adequate grounding.
Contact a technician to check the state of your electrical system. If there is no need to install additional
accessories, the instrument is now ready for use. Once positioned and installed with the necessary
components, the microscope is ready to be used. Your microscope is a laboratory instrument de-
signed to last. Handle it always carefully and avoid abrupt vibrations or shocks. Always disconnect the
power cable from the microscope when not in use for long time, while you clean it or when you perform
any maintenance.
AVOID DISASSEMBLING THE INSTRUMENT
Do not disassemble the instrument. This entails the cancellation of the warranty and may cause
malfunction.
4.1 Adjust the observation head
Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then
lock the lock-screw.
4.2 Place the specimen on the stage
Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is
centred over the stage opening by adjusting the coaxial knobs of the stage.
4.3 Epiuorescenceuse:AligningtheHglamp
- Move the lter selector of the lters block on the desired position, corresponding to an excitation
wavelength.
- Insert a specimen slide and focus with 4X objective.
- Close completely the Field Diaphragm by pulling down the lever marked “FS”. You should see the
diaphragm in the eld of view. Act on the pair of knobs on both sides in order to center the diaphragm.
3.0 UNPACKING AND ASSEMBLY

Page 9
- Fully open the Field Diaphragm. Now switch to a greater magnication objective and center the
lamp in order to maximize the light intensity (acting on the lamp centering knobs).
- Adjust the rotating lever for optimization of the lamp focusing.
CENTERING KNOB
FIELD DIAPHRAGM
LAMP CENTERING KNOBS
FOCUS ROTATING LEVER
- The lamp house allows also a ne tuning of the orientation of the mirror located at the
back of the lamp. Use the provided allen wrench to change the orientation.
SCREWS FOR MIRROR
ORIENTATION
For more information about the lamp, see section 5.
4.0 USING THE MICROSCOPE

Page 10
4.4 Always keep the iris diaphragm completely open when working with uorescence light.
4.5 Brightelduse:Lampsettings
The microscope is tted with a 3.5W X-LED3 illuminator. Insert the plug of the cable into the power
socket and turn on the switch on the back of the main body. Turn the brightness adjustment knob to a
brightness suitable for observation.
4.6 Adjust interpupillary distance
Hold the right and left parts of the observation head by both hands and adjust the interpupillary
distance by turning the two parts until one circle of light can be seen. After nishing the interpupillary
adjustment, turn the dioptric adjustment ring on the left eye tube to the “0” position at the graduation
line on the eyetube.
4.7 Focus and diopter adjustment
Loosen the focus-stop knob, rotate the coarse focusing knob to bring the slide into focus with an
objective with a low magnication, and then lock the focus-stop knob. Adjust the ne focusing knob to
get the image sharp and clear while observing with your right eye, then turn the left dioptric adjustment
ring to a sharp and clear image also with the other eye. Turn the tension-adjust-knob to get a suitable
tension for the focus system. When the image is in focus, turn the nosepiece to choose the objective
you need.
4.8 Brightelduse:Condenseradjustment
Turn the condenser adjustment knob to move the condenser up or down for a clear and uniform
sample illumination. The two condenser-centring screws can be used to centre the condenser.
4.9 Brightelduse:Setthenumericalaperture
Adjust the aperture of the iris diaphragm located under the condenser, to set the numerical aperture
of the illuminator, thus controlling image contrast and resolution.
4.10 Brightelduse:Fielddiaphragm
Adjust the eld diaphragm to align the system and to control stray light.
4.11 Brightelduse:Additionallters
The chromatic correction lter (blue) and the monochromator lter (green) can be inserted into the
lter holder for denition of specimen parts. The use of the green lter is recommended for phase
contrast observations.
4.12 Brightelduse:Usingthepolarizer(optional)
The polarizing set consists of two lters; the analyser and the polarizer. The analyser is placed in the
analyser holder (you have to remove the orange protection lter in order to access the holder) and
the polarizer on top of the illuminator.
4.13 Videocapturing(optional)
B-500TiFL is provided with trinocular observation head. It can be connected to cameras via a photo/
video adaptor, for photo and video capturing. Before taking a picture or lming video, if necessary,
obscure with a dark cloth both the camera viewnder and the eyepieces and pull out the light path
selector lever. Please refer to the adaptor and camera manuals for further details.
4.0 USING THE MICROSCOPE

Page 11
4.14 UsingthePhaseContrastset(optional)
The phase contrast set has 4 objectives for phase contrast and one condenser, with 4 rings of
phase, and one position for brighteld. To use the phase contrast, remove the Abbe condenser of the
microscope and replace it with the phase contrast condenser.
If, during use, the phase contrast diaphragm rings (anuli) are dislodged, they can be realigned rotating
the alignment screws placed on two sides of each ring, using the enclosed tools. In particular:
-Select an objective corresponding to the ring to be realigned, and focus on a slide under brighteld
illumination.
- The selector of the condenser has 5 selectable positions:
0 → to use when it is necessary to be used the instrument in brighteld
10 → to use with 10x phase contrast objective
20 → to use with 20x phase contrast objective
40 → to use with 40x phase contrast objective
100 → to use with 100x phase contrast objective
- Insert the phase ring to be realigned.
- Remove an eyepiece, and replace it with the centring telescope.
- Focus the centring telescope, without touching the microscope focus, in order to focus the phase ring
and the phase plate simultaneously. In particular, the phase plate is visible as a dark ring, and the
phase ring as a bright ring divided into sectors.
- Turning the two phase ring centring screws, position the phase ring (bright ring) so that it is
completely contained in the phase plate prole (dark ring). (See gure.)
- Remove the telescope, and replace the eyepiece.
4.15 Darkeldcondenser(optional)
For darkeld observation, the Abbe condenser in standard equipment must be replaced with a special
condenser for darkeld, showed in the picture.
4.0 USING THE MICROSCOPE

Page 12
5.0 MAINTENANCE
5.1 Always think about
- The following environment is required: Indoor temperature: 0-40°C, Maximum relative
humidity: 85 % (non condensing).
- Keep the microscope away from dust and shocks while in use.
- Turn off the light immediately after use.
- Use a soft lens tissue to clean the optics after use.
- Only if needed, use a cloth moistened with water and a mild detergent, rinsing with water
and drying immediately with a lint-free cloth.
- After use, cover the microscope with the included dust-cover, and keep it in a dry and clean place.
5.2 Do NOT!
- Wipe the surface of any optical items with your hands. Fingerprints can damage the optics.
- Use solvents, neither on the microscope, nor on the optics.
- Disassemble objective or eyepieces to attempt to clean them.
- Mishandle or impose unnecessary force on the microscope.
- Clean the unit with volatile solvents or abrasive cleaners.
- Attempt to service the microscope yourself.
5.3 Safetyprecautions-uorescencemercurylamp
- Always turn on the lamp with the current adjusted to maximum (5 A) and then wait for a minimum of
10 minutes before using the microscope.
- If the maximum current goes down below 4.5 A the lamp needs to be changed.
- After the lamp has been switched off it takes about 30 minutes before it is cool enough to
be ready to be used again.
- Be sure the lamp is cool before you cover the microscope with the dust-cover after use.
- The lamp has a lifetime of 500 hours. In order to avoid explosive failure, replace the lamp
after 500 hours, even if it seems to work correctly.
- The lamp light contains ultraviolet radiation that is harmful for the eyes and skin. Always look at the
lamp light through the orange shield or through the dark alignment window on the lter block.
5.4 Howtochangetheuorescencemercurylamp
Disconnect any cable from the epiilluminator. Loosen the lock-screw on the top of the lamp
housing. The lamp holder will come out with the door.

Page 13
Remove the old lamp or the plastic placeholder. Use protection glasses as, if handled carelessly,
there is a risk that the lamp may shatter and eject glass splinters. Thus, without touching the glass
part of the lamp, take out the uorescence lamp from its box, taking care not to exert stress on any
part of the lamp. Put one end inside the exible metal holder (observe that it ts only in one direction)
and then in the xed one. Tighten the screws just enough to make it stay inplace. If you tighten them
too much the lamp may shatter. Put the lamp door with lamp assembly back in place and connect the
cable on the side of the epiilluminator to the power supply. Connect the mains cable of the power sup-
ply. Turn on the power supply using the power switch. Wait until the current display shows about 5.0
A. If the current falls below 4.5A, replace the lamp. You should now wait for at least 10 minutes before
aligning the lamp.
On the life time display you can read for how many hours the lamp has been in use. Replace the lamp
every 500 hours, even if it seems to work correctly, as otherwise it may shatter. Take care to let the
lamp cool down at least 30 minutes before opening the lamp housing. After having replaced the lamp,
press the reset button to reset the life time count.
5.5 If you need to send the microscope to Optika for maintenance, please use the original packaging.
Input Voltage: 100 - 240 Vac, 50/60 Hz
BrighteldLamp: 3.5W X-LED3
Fluorescence Supply: 100 - 240 Vac, 50/60 Hz
Fluorescence Lamp: HBO 100 W high pressure mercury bulb
5.0 MAINTENANCE
6.0 ELECTRICAL SPECIFICATIONS

Page 14
Art.13 Dlsg 25 july 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relat-
ing to the reduction in the use of hazardous substances in electrical and electronic equipment and waste
disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste.
The separate collection of this equipment at the end of its lifetime is organized and managed by
the producer. The user will have to contact the manufacturer and follow the rules that he adopt-
ed for end-of-life equipment collection. The collection of the equipment for recycling, treatment
and environmentally compatible disposal, helps to prevent possible adverse effects on the envi-
ronment and health and promotes reuse and/or recycling of materials of the equipment. Improper
disposal of the product involves the application of administrative penalties as provided by the laws in force.
7.0 RECOVERY AND RECYCLING

Pagina 16
INDICE
1.0 DESCRIZIONE pag. 18
2.0 INTRODUZIONE pag. 20
3.0 DISIMBALLAGGIO E INSTALLAZIONE DEL MICROSCOPIO pag. 20
4.0 UTILIZZO DEL MICROSCOPIO pag. 23
5.0 MANUTENZIONE DEL MICROSCOPIO pag. 26
6.0 SPECIFICHE ELETTRICHE pag. 27
7.0 MISURE ECOLOGICHE pag. 28

Pagina 17
Il presente microscopio è uno strumento scientico di precisione studiato per durare molti anni con una manu-
tenzione minima, essendo costruito secondo i migliori standard ottici e meccanici e progettato per un utilizzo
quotidiano.
Optika ricorda che il presente manuale contiene informazioni importanti per un uso sicuro e una corretta ma-
nutenzione dello strumento. Esso deve quindi essere accessibile a chiunque lo utilizzi.
Optika declina ogni responsabilità derivante da un uso improprio dei suoi strumenti non indicato dalla pre-
sente guida.
 Avvertenzedisicurezza
Questo manuale contiene importanti informazioni e avvertenze riguardanti la sicurezza riguardo l’in-
stallazione, l’utilizzo e la manutenzione del microscopio. Si raccomanda di leggere attentamente il
manuale prima di qualsiasi utilizzo dello strumento. Per assicurare un utilizzo sicuro l’utente deve
leggere e seguire tutte le istruzioni poste nel presente manuale.
I prodotti OPTIKA sono studiati per un utilizzo sicuro in condizioni operative normali. Lo strumento e
gli accessori descritti nel manuale sono realizzati e testati secondo standard industriali di sicurezza
per strumentazione da laboratorio.
L’utilizzo non corretto può causare lesioni alla persona o danni allo strumento.
Mantenere questo manuale a portata di mano vicino allo strumento, per una facile consultazione.
 Precauzionidisicurezzaelettrica
Prima di collegare il cavo di alimentazione alla presa di rete, assicurarsi che la tensione di rete della
vostra regione corrisponda alla tensione di alimentazione dello strumento, e che l’interruttore dell’illu-
minatore sia in posizione spenta.
L’utente deve osservare la regolamentazione riguardante la sicurezza in vigore nel proprio Stato. Lo
strumento è dotato di marcatura di sicurezza CE, in ogni caso l’utente ha piena responsabilità riguar-
do all’utilizzo sicuro dello strumento stesso.
 Simbolidiavvertenza/pericolousatinelmanuale
L’utente deve essere a conoscenza degli aspetti legati alla sicurezza nel momento in cui utilizza lo
strumento. I simboli di avvertenza o pericolo sono indicati sotto. Tali simboli sono utilizzati in questo
manuale di istruzioni.
Seguire le istruzioni contrassegnate da questo simbolo per evitare possibili gra-
vi danni alle persone.
Avvertimento di utilizzo; la non corretta operazione sullo strumento può causare
danni alla persona o allo strumento.
Possibilità di shock elettrico.
Attenzione: superci ad elevata temperatura. Evitare il contatto diretto.
Note tecniche o consigli di utilizzo.
INDICAZIONI PER LA SICUREZZA

Pagina 18
1.0 DESCRIZIONE
ALIMENTATORE
BLOCCO FILTRI
MANOPOLE DI REGOLAZIONE
DELLA LAMPADINA
PORTA-LAMPADINA HG
PORTA FOTO
TESTATA DI
OSSERVAZIONE
OCULARE
ANELLO
DI COMPENSAZIONE
DIOTTRICA
REVOLVER
PORTAOBIETTIVI
OBIETTIVI
TAVOLO PORTAPREPARATI
CON PINZETTA PER
SOSTEGNO CAMPIONI
DIAFRAMMA DI
APERTURA (CAMPO
CHIARO)
DIAFRAMMA DI CAMPO
(CAMPO CHIARO)
MANOPOLA DI COMANDO
DELLA MESSA A FUOCO
MACROMETRICA
MANOPOLA DI COMANDO
DELLA MESSA A FUOCO
MICROMETRICA

Pagina 19
1.0 DESCRIZIONE
COMPONENTI SPECIFICHE PER L’ILLUMINAZIONE IN CAMPO CHIARO
CONDENSATORE
VITI DI CENTRATURA DEL
CONDENSATORE
MANOPOLE PER TAVOLO
TRASLATORE
MANOPOLA DI REGOLAZIONE
DEL CONDENSATORE
LEVETTA PER SELEZIONE PERCORSO
OTTICO
USCITA TRINOCULARE PER ATTACCO
ADATTATORE FOTO/VIDEO

Pagina 20
2.0 INTRODUZIONE
Questo microscopio è uno strumento scientico di precisione duraturo negli anni e con una manutenzione
minima. Viene costruito secondo i migliori standard ottici e meccanici ed è resistente ad un utilizzo quotidiano
in aula o in laboratorio.Optika ricorda che questo manuale contiene informazioni importanti per un uso sicuro
ed una manutenzione corretta del microscopio. Esso dovrebbe quindi essere visionato da chiunque lo utilizzi.
Optika declina ogni responsabilità circa i suoi strumenti in caso di uso improprio e non contemplato dal
presente manuale.
3.1 Il microscopio è imballato in uno stampo di polistirolo. Aprire la scatola e rimuovere la parte superiore
dello stampo. Fare attenzione a non far cadere e danneggiare le componenti ottiche (obiettivi e
oculari). Estrarre il microscopio dalla scatola usando entrambe le mani (una per il braccio e una per la
base) ed appoggiarlo su di un piano stabile.
3.2 Gli obiettivi sono imballati singolarmente in custodie di protezione. Estrarre gli obiettivi dalle loro
custodie e inserirli in ordine nel revolver portaobiettivi, dall’obiettivo con minor potere d’ingrandimento
a quello più potente, in direzione oraria partendo dal retro.
3.3 Le componenti epiuorescenti (epiilluminatore con blocco ltri, alimentatore e porta-lampada al
mercurio) si trovano in uno scompartimento rigido. Togliere i tappi di protezione situati sopra lo stativo
e l’epiilluminatore del B-600 e posizionare l’epiilluminatore sulla parte superiore dello stativo. Stringere
la vite posta sul lato destro con il cacciavite in dotazione.
3.4 Fissare la testata di osservazione sulla parte superiore dell’epiilluminatore mediante l’apposita vite di
serraggio.
3.5 Inserire il porta-lampada sul retro dell’epiilluminatore e stringere la vite con il cacciavite in dotazione.
3.0 DISIMBALLAGGIO E INSTALLAZIONE
VITE
DELL’EPIILLUMINATORE
VITE DEL PORTA-LAMPADA