PerkinElmer LS 55 User manual

LS 55
User’s Guide

Release History
Part Number Release Publication Date
09934436 C March 2007
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer Ltd.
Chalfont Road
Seer Green
Beaconsfield
Bucks
HP9 2FX
United Kingdom
Or emailed to: [email protected]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer
makes no warranty of any kind with regard to this document, including, but not
limited to, the implied warranties of merchantability and fitness for a particular
purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential
damages in connection with furnishing, performance or use of this material.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form whatsoever
or translated into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2007 PerkinElmer, Inc.
Produced in the UK.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked
as such, are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.
FL WinLab is a trademark of PerkinElmer, Inc.
Windows is a trademark of the Microsoft Corporation.
Posidriv is a registered trademark of the Phillips Screw Company.

LS 55 User’s Guide . 3
Contents
Contents.................................................................................................... 3
Warnings and Safety Information...................................................... 7
Safety Information ..................................................................................... 8
Environmental conditions for safe operation .......................................... 8
Warning Labels on the Instrument ............................................................ 10
Further assistance.............................................................................. 11
Introduction ..................................................................................... 13
About this Manual .................................................................................... 14
Conventions ...................................................................................... 14
Definitions......................................................................................... 17
Specifications of the LS 55........................................................................ 18
Unpacking and Installation .............................................................. 21
Introduction............................................................................................. 22
Shipping kit list ........................................................................................ 24
Lifting the LS 55 Luminescence Spectrometer ............................................ 25
Removal of the shipping clamps................................................................ 26
Electrical Connections............................................................................... 29
Connection to electrical mains supply.................................................. 29
Accessory connectors......................................................................... 29
Rear panel connectors........................................................................ 30
Switching on the LS 55............................................................................. 34
Accessories: Installation and Use .................................................... 37
Single Position Cellholder (standard) ......................................................... 38
Description ........................................................................................ 38
Installation and maintenance.............................................................. 38
Stirred Single Position Cellholder ............................................................... 39
Description ........................................................................................ 39
Installation and maintenance.............................................................. 39
Operation.......................................................................................... 39
Stirred Four-Position Cellchanger .............................................................. 40
Description ........................................................................................ 40
Installation and maintenance.............................................................. 40
Biokinetics Accessory................................................................................ 41
Description ........................................................................................ 41
Installation and maintenance.............................................................. 41
Operation.......................................................................................... 42
Screw-fitting Flowcell ............................................................................... 43
Description ........................................................................................ 43
Installation and maintenance.............................................................. 44
Operation.......................................................................................... 44
Installing Thermostatting Tubing to Cellholders.......................................... 45
The four-position cellchanger ............................................................. 47

4 . LS 55 User’s Guide
Using the Septum Injector ........................................................................48
Using Stirrer Bars .....................................................................................50
Minimum volumes with various cuvette types............................................. 51
Semi-micro Cuvette and Holder ................................................................. 52
Description ........................................................................................52
Installation ........................................................................................52
Stirred Semi-micro Cuvette .......................................................................53
Description ........................................................................................53
Operation ..........................................................................................54
Care of Optical Mirrors Inside Cellholder Accessories ..................................55
Resistance to Solvents ..............................................................................56
Sipper......................................................................................................57
Description ........................................................................................57
Installation and maintenance .............................................................. 58
Operation ..........................................................................................60
LC Flowcell...............................................................................................63
Description ........................................................................................63
Installation ........................................................................................63
Operation ..........................................................................................65
Front Surface Accessory............................................................................ 66
Description ........................................................................................66
Installation and maintenance .............................................................. 67
Operation ..........................................................................................70
Using Powders ...................................................................................71
Plate Reader ............................................................................................73
Description ........................................................................................73
Installation ........................................................................................73
Operation ..........................................................................................80
The Well Plate Reader application .......................................................80
The TLC Scan application.................................................................... 81
Measuring TLC plates, gels or other flat samples.................................. 82
Red-sensitive Detector.............................................................................. 83
Description ........................................................................................83
Installation ........................................................................................84
Total Emission Accessory .......................................................................... 85
Description ........................................................................................85
Installation ........................................................................................85
Operation ..........................................................................................86
External Fibre Optic Accessory .................................................................. 87
Description ........................................................................................87
Installation ........................................................................................88
Operation ..........................................................................................92
The Fast Filter Accessory ..........................................................................94
Description ........................................................................................94
Installation ........................................................................................96
Operation ........................................................................................ 104

LS 55 User’s Guide . 5
The Polarizer Accessory .......................................................................... 105
Description ...................................................................................... 105
Installation ...................................................................................... 106
Bit Switches..................................................................................... 110
Operation........................................................................................ 110
The Low Temperature Accessory............................................................. 111
Description ...................................................................................... 111
Installation ...................................................................................... 112
Filling the accessory with liquid nitrogen ........................................... 117
Operation........................................................................................ 118
Removing the accessory................................................................... 119
Accessory spares.................................................................................... 120
RS232 Commands .......................................................................... 121
Introduction........................................................................................... 122
Section 1- RS232C Communications ........................................................ 125
Monochromator Drive....................................................................... 127
Plate Reader Driver.......................................................................... 127
Source Control and Photomultiplier Control........................................ 128
Slit and Filter Motors ........................................................................ 128
Analogue Output.............................................................................. 128
Sample Acquisition Accessories......................................................... 129
Fast Filter Accessory (FFA) ............................................................... 129
Section 2 - Command Formats ................................................................ 131
Monochromator Scan Control Commands .......................................... 133
Time Drive Commands ..................................................................... 136
TLC Scan Commands ....................................................................... 139
Data Collection/Processing Set Up Commands ................................... 142
Data Output Control Commands ....................................................... 147
Motor Control .................................................................................. 148
Calibration Commands ..................................................................... 151
Miscellaneous Commands................................................................. 152
Plug-in Accessory Control Commands................................................ 155
Well Plate Reader (WPR) Commands ................................................ 157
Fast Kinetics Application (FKA) Commands ........................................ 158
Fast Filter Accessory (FFA) Commands .............................................. 161
System Description and Maintenance ............................................ 163
The Optical System ................................................................................ 164
The Electronics System and Signal Handling ............................................ 166
Instrument Modes............................................................................ 167
Signal Handling Diagram .................................................................. 170
Fuses .................................................................................................... 171
Other Fuses..................................................................................... 172
Removing the Main Cover....................................................................... 173
Refitting the main cover ................................................................... 175
Removing the Main Optical Cover............................................................ 176
Refitting the optical cover................................................................. 178

6 . LS 55 User’s Guide
Customizing filter wheels ........................................................................ 179
Changing the Lamp ................................................................................ 181
Preparing the LS 55 for transport ............................................................ 188
Inserting the clamps ........................................................................ 188
Lifting the LS 55 Luminescence Spectrometer .................................... 191
Removing the clamps ....................................................................... 191
Cleaning the LS 55 ................................................................................. 192
Cleaning the air filter .............................................................................. 193
Changing the Instrument Operating Voltage ............................................ 194
Changing the mains fuse .................................................................. 196
The mains voltage plate ................................................................... 197
Index ..............................................................................................199
Index..................................................................................................... 200

Warnings and Safety
Information

8 . LS 55 User’s Guide
Safety Information
The LS 55 Luminescence Spectrometer has been designed and tested in accordance
with PerkinElmer Specifications and IEC 61010-1, 'Safety requirements for electrical
equipment for measurement, control and laboratory use'.
This apparatus is protected in accordance with IEC Class 1 rating. This manual
contains information and warnings that must be followed by the operator to ensure
safe operation and to retain the instrument in a safe condition.
The instrument has been designed for indoor use and will operate correctly under
the following conditions:
Ambient temperature 15 °C to 35 °C
Relative humidity 75% maximum, non-condensing
Environmental conditions for safe operation
The LS 55 has been designed to be safe under the following conditions:
•Indoor use.
•Altitude up to 2000 m.
•Temperature range 5 °C to 40 °C.
•Maximum relative humidity 80% for temperatures up to 31 °C, decreasing
linearly to 50% relative humidity at 40 °C.
•Mains voltage fluctuations not to exceed 10% of the nominal voltage.
•An IEC Installation Category II (Overvoltage Category II) classification, suitable
for connection to local level power supplies.
•An IEC Pollution Degree 2 classification: normally only non-conductive pollution
occurs, occasionally, however, a temporary conductivity caused by
condensation must be expected.
•An IEC Insulation Class 1 rating for external circuits – only connect equipment
that meets the requirements of IEC 61010-1, IEC 60950 or equivalent
standards.

Warnings and Safety Information . 9
WARNING
This equipment must be earthed (grounded).
Any interruption of the protective conductor, inside or outside the instrument, or
disconnection of the protective earth terminal is likely to make the instrument
dangerous.
When the instrument is connected to the mains supply, terminals may be
hazardous when live and the opening of covers or the removal of parts (except
those to which access can be gained by hand) is likely to expose live parts. Any
adjustment, maintenance and repair of the opened operating instrument must be
carried out only by a skilled person who is aware of the hazards involved (a
RESPONSIBLE BODY). The instrument must be disconnected from all voltage
sources before it is opened for any adjustment, replacement, maintenance or
repair.
Capacitors inside the instrument may still be charged even if the instrument has
been disconnected from all voltage sources. Only fuses with the required current
and voltage rating and of the specified type are to be used for replacement.
Makeshift fuses must NOT be used and fuse holders must not be short-circuited.
Whenever it is likely that the protection has been impaired, the instrument must be
made inoperative and secured against any unauthorized operation. The protection
is likely to be impaired, for example, when the instrument:
•Shows visible damage;
•Fails to perform the intended measurement;
•Has been subjected to prolonged storage under unfavorable conditions;
•Has been subjected to severe transport stresses.
NOTE: Using the equipment in a manner not specified in this User’s Guide may also
impair the protection provided by the equipment.

10 . LS 55 User’s Guide
Warning Labels on the Instrument
Figure 1 Caution label
When the Caution label (Figure 1) is attached to the instrument it means ‘Caution,
risk of danger’. Refer to this User’s Guide to find out the nature of the potential
hazard and any actions which have to be taken.
There are three Caution labels on the instrument:
•In the sample area – where the symbol denotes that there is a possibility of
eye damage, and you must not stare into the beam in the sample
compartment.
•Near the mains inlet fuse – where the symbol denotes that the operator
replaceable fuse (see
Fuses on page 171) is not an IEC fuse, but a CSA
approved fuse.
•By the left hand screw that fixes the top cover (at the front left of the
instrument) – where the symbol denotes that removing this cover may expose
hazardous voltages. Any adjustment, maintenance, or repair of the opened,
operating instrument, must be performed by a skilled person who is aware of
the hazards involved (a RESPONSIBLE BODY).
Figure 2 High voltage label
The high voltage label (see Figure 2) means that removing this cover will give
access to hazardous voltages.

Warnings and Safety Information . 11
There are two High Voltage labels on the instrument:
•Inside the instrument on top of the source cover.
•Inside the instrument on top of the monochromator cover (on the right side of
the instrument).
Both these labels mean that the instrument must be switched off, and the mains
supply lead removed from its socket before the cover is removed. Any adjustment,
maintenance, or repair of the opened, operating instrument, must be performed by
a skilled person who is aware of the hazards involved (a RESPONSIBLE BODY).
Further assistance
For technical assistance, please contact your local PerkinElmer office or agent, or
the address at the front of this manual.

12 . LS 55 User’s Guide

Introduction

14 . LS 55 User’s Guide
About this Manual
This LS 55 User's Guide describes specifications and the installation of the
LS 55 Luminescence spectrometer and its accessories.
Information about the FL WinLab Software package can be found in the
FL WinLab Software User's Guide , which is delivered with the instrument.
Conventions
The following conventions are used in this manual:
•Normal text is used to provide information and instructions.
•Bold text refers to text that is displayed on the PC screen.
•UPPERCASE text, for example ENTER or ALT, refers to keys on the PC
keyboard. '+' is used to show that you have to press two keys at the same
time, for example, ALT+F.
•All eight character ‘numbers’, for example L2250140, are PerkinElmer part
numbers unless stated otherwise.
Notes, warnings and cautions
Three terms, in the following standard formats, are also used to highlight special
circumstances and warnings.
NOTE: A note indicates additional, significant information that is provided with
some procedures.

Introduction . 15
WARNING
We use the term WARNING to inform you about situations that
could result in personal injury to yourself or other persons.
Details about these circumstances are in a box like this one.
D
Warning (Warnung)
Bedeutet, daß es bei Nichtbeachten der genannten Anweisung
zu einer Verletzung des Benutzers kommen kann.
DK
Warning (Advarsel)
Betyder, at brugeren kan blive kvæstet, hvis anvisningen ikke
overholdes.
E
Warning (Peligro)
Utilizamos el término WARNING (PELIGRO) para informarle
sobre situaciones que pueden provocar daños personales a
usted o a otras personas. En los recuadros como éste se
proporciona información sobre este tipo de circunstancias.
F
Warning (Danger)
Nous utilisons la formule WARNING (DANGER) pour avertir
des situations pouvant occasionner des dommages
corporels à l'utilisateur ou à d'autres personnes. Les détails
sur ces circonstances sont données dans un encadré semblable
à celui-ci.
I
Warning (Pericolo)
Con il termine WARNING (PERICOLO) vengono segnalate
situazioni che potrebbero provocare incidenti alle persone.
Troverete informazioni su tali circostanze in un riquadro come
questo.
NL
Warning (Waarschuwing)
Betekent dat, wanneer de genoemde aanwijzing niet in acht
wordt genomen, dit kan leiden tot verwondingen van de
gebruiker.
P
Warning (Aviso)
Significa que a não observância da instrução referida poderá
causar um ferimento ao usuário.

16 . LS 55 User’s Guide
CAUTION
We use the term CAUTION to inform you about situations that
could result in serious damage to the instrument or other
equipment. Details about these circumstances are in a box like
this one.
D
Caution (Achtung)
Bedeutet, daß die genannte Anleitung genau befolgt werden
muß, um einen Geräteschaden zu vermeiden.
DK
Caution (Bemærk)
Dette betyder, at den nævnte vejledning skal overholdes nøje
for at undgå en beskadigelse af apparatet.
E
Caution (Advertencia)
Utilizamos el término CAUTION (ADVERTENCIA) para advertir
sobre situaciones que pueden provocar averías graves en
este equipo o en otros. En recuadros éste se proporciona
información sobre este tipo de circunstancias.
F
Caution (Attention)
Nous utilisons le terme CAUTION (ATTENTION) pour signaler
les situations susceptibles de provoquer de graves
détériorations de l'instrument ou d'autre matériel. Les
détails sur ces circonstances figurent dans un encadré
semblable à celui-ci.
I
Caution (Attenzione)
Con il termine CAUTION (ATTENZIONE) vengono segnalate
situazioni che potrebbero arrecare gravi danni allo
strumento o ad altra apparecchiatura. Troverete informazioni
su tali circostanze in un riquadro come questo.
NL
Caution (Opgelet)
Betekent dat de genoemde handleiding nauwkeurig moet
worden opgevolgd, om beschadiging van het instrument
te voorkomen.
P
Caution (Atenção)
Significa que a instrução referida tem de ser respeitada para
evitar a danificação do aparelho.

Introduction . 17
Definitions
OPERATOR: Person operating the equipment for its intended purpose.
RESPONSIBLE BODY: Individual or group responsible for the use and
maintenance of the equipment and for ensuring that the OPERATORS are
adequately trained.

18 . LS 55 User’s Guide
Specifications of the LS 55
Principle: Computer controlled ratioing luminescence spectrometer with the
capability of measuring fluorescence, phosphorescence, chemiluminescence and
bioluminescence.
Source: Xenon discharge lamp, equivalent to 20 kW for 8 μs duration. Pulse width
at half height <10 μs.
Sample detector: Gated photomultiplier with modified S5 response for operation up
to around 650 nm. Red-sensitive R928 photomultiplier can be optionally fitted for
operation up to 900 nm.
Reference detector: Photodiode for operation up to around 900 nm.
Monochromators: Monk-Gillieson type monochromators cover the following ranges:
•Excitation 200-800 nm with zero order selectable.
•Emission 200-650 nm with standard photomultiplier with zero order
selectable, 200-900 nm with optional R928 photomultiplier.
Synchronous scanning is available with constant wavelength or constant energy
difference.
Wavelength accuracy: +1.0 nm
Wavelength reproducibility: +0.5 nm
Spectral bandpass: The excitation slits (2.5-15.0 nm) and emission slits
(2.5-20.0 nm) can be varied and selected in 0.1 nm increments.
Phosphorescence mode: Delay and gate times can be varied to measure
phosphorescence. Minimum total period 13.0 ms (50 Hz)
Scanning speed: Scanning speed can be selected in increments of 1 nm for
10-1500 nm/min. Time-dependent data can also be collected.
Emission filters: Computer selectable cut-off (high-pass) filters at 290, 350, 390,
430 and 515 nm, a blank (to act as shutter), a 1% T attenuator and clear beam
position.
Sensitivity: Signal to noise is 500:1 r.m.s., using the Raman band of water with
excitation at 350 nm, excitation and emission bandpass 10 nm.
Standard cellholder: A single position water thermostattable holder for 10 mm
cuvettes.

Introduction . 19
Supply Voltages: 100 V / 120 V / 220 V / 230 V
Frequency: 50 / 60 Hz
Power: 150 VA

20 . LS 55 User’s Guide