PerkinElmer IVIS Lumina XRMS III Series User manual

PN CLS137706 RevB
IVIS®Lumina XRMS Series III
December 2013
HARDWARE MANUAL

PerkinElmer Health Sciences
940 Winter Street, Waltham, Massachusetts 02451 USA
Telephone: [phone] (US) or +1.203.925.4602
Fax: [phone]
For sales: [email protected]
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877.522.497.3302 (US) or +1.508.435.9500
www.PerkinElmerls.com
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Copyright
© 2013 PerkinElmer Health Sciences, Inc. and its parent, affiliated, and subsidiary companies. All rights reserved, including
but not limited to those rights to reproduce this publication or parts thereof.

Contents
Chapter 1 Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Chapter 2 Important Safety Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
2.1 Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
2.2 Safety Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
2.3 Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Read, Retain, and Follow Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Heed Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2.4 X-Ray Safety and Hazards: Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
2.5 Environmental Considerations for the System Components . . . . . . . . . . . . . . . .5
Location for the IVIS Lumina XRMS Series III . . . . . . . . . . . . . . . . . . . . . . . . . 5
Heat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Water and Moisture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.6 Cleaning or Moving the System Components . . . . . . . . . . . . . . . . . . . . . . . . 5
Cleaning/Liquid Entry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Moving the IVIS Lumina XRMS Series III . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
2.7 Power Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Power Sources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Power Cord Protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Lightning and Power Line Surges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Power Outages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Overloading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
2.8 Servicing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.9 Other Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 3 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.1 Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.2 X-Ray Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.3 Eye Safety and Burn Hazard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.4 Mechanical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.5 Chemical and Biological Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.6 Panels, Cover, and Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Chapter 4 Legal Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.2 Limited Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.3 Patents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4.4 Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.5 Disclaimers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Chapter 5 X-ray Safety and Radiation Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

IVIS®Lumina XRMS Series III Hardware Manual Contents ii
Cabinet X-ray System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Other Product Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5.2 Radiation Theory and X-ray Generation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Types of Radiation: Ionizing and Non-ionizing . . . . . . . . . . . . . . . . . . . . . . . 14
X-rays: An Ionizing Radiation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
X-rays: A Penetrating Radiation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
X-rays: How They are Made . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Lumina XRMS Series III X-ray Source Tube . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.3 Biological Effects of Radiation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Ionization Process and the Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Deterministic Effects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Stochastic Effects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
X-ray Dose Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.4 IVIS Lumina XRMS Series III Safety Systems . . . . . . . . . . . . . . . . . . . . . . . . 17
Radiation Shielding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
IVIS Lumina XRMS Series III Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . 18
Requirements for Turning on X-rays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Turning Off X-rays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
X-ray ON Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Safety Interlocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Safety Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.5 Your Laboratory X-ray Safety Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Contact Your Radiation Protection Authorities . . . . . . . . . . . . . . . . . . . . . . . 22
Study Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Create Radiation Safety Plan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Create a Training Plan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Safety Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Regulatory Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.6 Contact Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
PerkinElmer Sales and Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . 24
US State Radiation Authorities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Canadian Radiation Authorities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Chapter 6 Components and Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
6.1 CCD Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CCD Camera Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CCD Camera Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
6.2 X-Ray System and Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
6.3 X-Ray Scintillation Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.4 X-Ray System Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
6.5 Key Selector Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
6.6 Imaging Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Imaging Chamber Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Imaging Chamber Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.7 Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.8 Optical Filter Wheel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

IVIS®Lumina XRMS Series III Hardware Manual Contents iii
6.9 Acquisition Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Computer Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
6.10 Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Chapter 7 Basic Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
7.1 Starting the IVIS Lumina XRMS Series III . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
7.2 Restarting the System After a Power Outage . . . . . . . . . . . . . . . . . . . . . . . . 46
7.3 Gas Plumbing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
7.4 Door Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Stage Curtain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
7.5 Changing the X-Ray Scintillation Plate Position . . . . . . . . . . . . . . . . . . . . . . 49
Moving the Scintillation Plate to the Upper Position . . . . . . . . . . . . . . . . . . 49
Moving the Scintillation Plate to the Lower Position . . . . . . . . . . . . . . . . . . 52
7.6 Imaging Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Black Paper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Centering a Subject . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Glowing Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
7.7 System Shut Down Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Chapter 8 Fluorescence Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
8.1 About the Fluorescence Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
8.2 Installation Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
8.3 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Electrical Power and Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Lamp and Fuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Ventilation Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Chemicals Required for Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Weight and Dimensions of the Fluorescence Light Source Module . . . . . . 58
8.4 Description and Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
System Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Understanding Filter Spectra . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
8.5 Fluorescent Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
IVIS Acquisition Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Acquiring Fluorescent Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
8.6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Hardware Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Fuse Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Lamp Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
8.7 Care and Maintenance of the Fluorescence Equipment . . . . . . . . . . . . . . . . . . . .67
Cleaning the Fluorescence Light Source Module . . . . . . . . . . . . . . . . . . . . . 67
Cleaning the IVIS Lumina XRMS Series III and Fluorescence Equipment . . 67
Cleaning the Optical Components and Filter Replacement . . . . . . . . . . . . . 67
Chapter 9 Care and Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
9.1 Surveying the IVIS Lumina XRMS Series III for Radiation Leakage . . . . . . . 68

IVIS®Lumina XRMS Series III Hardware Manual Contents iv
Conducting the X-Ray Radiation Survey . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
9.2 Maintenance and Safety Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Daily Safety Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Weekly Safety Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Monthly Safety Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Annual Safety Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
9.3 Cleaning the IVIS Lumina XRMS Series III . . . . . . . . . . . . . . . . . . . . . . . . . . 69
9.4 Cleaning the Scintillation Plate Window and Holder . . . . . . . . . . . . . . . . . . 70
Chapter 10 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.1 Measured Temperature Is Not Equal to Demand Temperature . . . . . . . . . . 74
10.2 Photographic Image Is Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
10.3 Luminescent Image Is Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
10.4 No Image Produced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Appendix A XWS-260 Workstation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
A.1 Shutting Down the Imaging System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78
A.2 Moving the Imaging System on the XWS-260 Workstation . . . . . . . . . . . . . 78
A.3 Starting the Imaging System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Appendix B Options and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Appendix C Scintillation Plate Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
C.1 Removing the Scintillation Plate Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
C.2 Replacing the Scintillation Plate Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

1 Welcome
The IVIS®Lumina XRMS Series III is a high-sensitivity, low noise imaging system providing in vivo
fluorescent and bioluminescent imaging together with X-ray capability (Figure 1.1).
The IVIS Lumina XRMS Series III Imaging System includes:
A scientific grade, thermoelectrically-cooled CCD camera mounted on a light-tight imaging
chamber with integrated optical modules.
A module for X-ray imaging with a two-position scintillation plate that accommodates subjects up
to 2.8 cm vertical height or larger subjects up to 5.3 cm vertical height.
Fluorescence equipment:
22-position excitation filter wheel with 19 equally spaced excitation filters covering the
wavelength range from 410 to 790 nm
8-position filter wheel with 7 emission filters spanning the wavelength range from 500 – 865 nm
Living Image®Software for automated image acquisition, post-processing, and data analysis.
A Windows®-based computer system for data acquisition and analysis.
This manual explains how to operate and maintain the equipment, and provides guidelines for
obtaining optimal bioluminescent, fluorescent, and X-ray images. Before using the IVIS Lumina
XRMS Series III, please read this manual carefully to obtain safe, optimum performance and a
maximum service life from the unit. For instructions on using the Living Image software, please see
the Living Image®Software User Manual for IVIS Lumina XRMS Series III (PN CLS137702RevB).
If you have questions regarding this manual or the IVIS Lumina XRMS Series III, please contact
PerkinElmer Technical Support (see page 24).
Figure 1.1 IVIS Lumina XRMS Series III

2 Important Safety Instructions
Safety Information
Safety Symbols on page 3
Instructions on page 3
X-Ray Safety and Hazards: Regulations on page 4
Environmental Considerations for the System Components on page 5
Cleaning or Moving the System Components on page 5
Power Considerations on page 6
Servicing on page 7
Other Equipment on page 7
2.1 Safety Information
This manual provides safety information in the following formats:
!
CAUTION: A caution note indicates a potentially hazardous situation which, if not
avoided, may result in minor or moderate injury and/or mechanical damage. It is
also used to alert you to unsafe practices. It reminds you that all safety
instructions should be read and understood before installation, operation,
maintenance, or repair of this instrument. When you see this symbol, pay
particular attention to the safety information presented. Observance of safety
precautions will help avoid actions that could damage or adversely affect the
performance of the IVIS Lumina XRMS Series III. If the equipment is used in a
manner not specified in this manual, the protection provided by the equipment
may be impaired.
!
WARNING! Used when an action or condition may potentially cause serious
personal injury or loss of life. Mechanical damage may also result.
VOLTAGE! Provides safety information about high voltage or risk of electric
shock.

IVIS®Lumina XRMS Series III Hardware Manual Chapter 2 | Important Safety Instructions 3
2.2 Safety Symbols
Table 2.1 shows safety symbols that are found on the IVIS Lumina XRMS Series III and in this
manual.
2.3 Instructions
Read, Retain, and Follow Instructions
Read and understand all the safety and operating instructions before you install, operate, or perform
maintenance on the IVIS Lumina XRMS Series III. Make sure that you fully understand the
following safety instructions, warnings, and disclaimers before proceeding to the rest of the manual.
Retain the safety and operating instructions for future reference.
Follow all operating and handling instructions. Failure to follow operating or handling instructions
may void any warranty covering this product.
Heed Warnings
Abide by all warnings on the product and in the operating instructions. Failure to adhere to warnings
or safety precautions may void any warranty covering the IVIS Lumina XRMS Series III.
Table 2.1 Safety symbols
Symbol Definition
Warning: Hazardous voltage
Warning: The equipment produces X-rays when energized.
Warning (Canada): The equipment produces X-rays when energized.
!
WARNING! The IVIS Lumina XRMS Series III should be operated only by personnel
who have been trained in radiation safety and the operation and safety
instructions contained in this manual. PerkinElmer also recommends that
personnel who operate the equipment, or are close proximity to the equipment,
use a radiation film badge or other type of appropriate personal dosimeter

IVIS®Lumina XRMS Series III Hardware Manual Chapter 2 | Important Safety Instructions 4
2.4 X-Ray Safety and Hazards: Regulations
This equipment produces X-rays when energized. Before operating the equipment, read and
understand the specific information in X-ray Safety and Radiation Hazards on page 14. DO NOT
operate the IVIS Lumina XRMS Series III unless an X-ray safety survey has been performed within
the last 12 months. Please contact PerkinElmer Technical Support for more information (see
page 24).
An X-ray safety survey must be performed when the instrument is installed or after it has been
moved, unless the instrument was moved on the XWS-260 Workstation cart and no abnormal
mechanical shocks occurred. A survey is also to be performed when the IVIS Lumina XRMS Series
III has undergone any form of service in which the electronics drawer has been opened, the safety
interlocks have been adjusted or any of the shielding has been removed and re-installed.
After servicing, if the safety interlocks are not operating properly or if the X-ray shielding is not
properly re-installed, serious injury can result when operating the system. Conducting an X-ray
safety survey is the only way to confirm proper shielding and interlock operation.
Owners and operators of the IVIS Lumina XRMS Series III are responsible for complying with all
regulations in the country where the equipment is operated. This includes all local, state, and federal
regulations. In some states of the US, it may be necessary to register radiation sources with the
governing state and/or local public health agencies before operating the instrument. Equipment
registration may be required immediately or within 30 days of acquiring the equipment.
Owners and operators of the IVIS Lumina XRMS Series III are responsible for contacting the
appropriate public health agencies for registration information that pertains to installation of the IVIS
Lumina XRMS Series III. If you need assistance with this requirement, contact PerkinElmer
Technical Support (see page 24). For more details and contact information, see Safe Operating and
Emergency Procedures for the Operation of the IVIS Lumina XRMS Series III Cabinet X-Ray System.
This document was provided with the pre-installation instructions.
!
WARNING! For radiation survey of the IVIS Lumina XRMS Series III, please comply
with your own laboratory radiation regulations or contact PerkinElmer Technical
Support for further assistance
!
WARNING! A PerkinElmer representative will conduct a radiation leakage survey
and safety tests when the IVIS Lumina XRMS Series III is installed. PerkinElmer
representatives are trained in radiation safety. However, check with your local
radiation control authority to determine the specific radiation survey
requirements at your facility. If necessary, have a qualified expert other than a
PerkinElmer representative survey the installation before operating the
instrument.

IVIS®Lumina XRMS Series III Hardware Manual Chapter 2 | Important Safety Instructions 5
2.5 Environmental Considerations for the System Components
Location for the IVIS Lumina XRMS Series III
Before the IVIS Lumina XRMS Series III is installed, consider the proper environment for the
components. Install the equipment in an environment where:
The temperature does not fluctuate widely and is maintained between 15-25 °C (59-77 °F).
The humidity does not exceed 80%.
No strong electric or magnetic fields exist.
No vibrations are present.
No corrosive gases are present.
High amounts of dust are not present.
No open flame is present.
There is sufficient space behind the IVIS Lumina XRMS Series III equipment. A minimum space
of four inches from the flat surface of the rear panel should be provided behind the IVIS Lumina
XRMS Series III to provide unobstructed air flow and access to the main power on/off switch.
The work space is level.
Heat
The system should be situated away from heat sources such as open flames, radiators, heat registers,
stoves, and other heat-generating electrical equipment.
Water and Moisture
2.6 Cleaning or Moving the System Components
Cleaning/Liquid Entry
VOLTAGE! Do not use this product near water (for example, near a sink or wet
room) due to risk of electric shock, electrical damage, and/or equipment failure.
VOLTAGE! Do not use liquid or aerosol cleaners and never spill liquid of any kind
on any of the IVIS Lumina XRMS Series III components. Sprays and liquids that
come into contact with the IVIS Lumina XRMS Series III hardware may result in
damage to the system or electrocution. For more details on proper care of the
system, see Care and Maintenance on page 68.

IVIS®Lumina XRMS Series III Hardware Manual Chapter 2 | Important Safety Instructions 6
Moving the IVIS Lumina XRMS Series III
You can move the light source module on the laboratory bench within the extent of the fiber optic
cable. Be careful not to bend the fiber optic cable (minimum radius of bending: 3 inches/7.6 cm).
2.7 Power Considerations
Power Sources
The IVIS Lumina XRMS Series III is configured for the voltage requirements of the installation
locality that was specified at the time of order. If the IVIS Lumina XRMS Series III is moved to
another area, make sure that the same voltage requirements exist.
Power Cord Protection
Power supply cords should be routed so that they are unlikely to be walked on or pinched by items
placed upon or against them. Pay close attention to receptacles and to points of connection between
cords and equipment.
Lightning and Power Line Surges
The IVIS Lumina XRMS Series III is supplied with a surge protector. All components should be
connected to this device to protect against electrical transient events. Failure to isolate the camera
from electrical transients may result in damage to the CCD camera.
Power Outages
If the IVIS Lumina XRMS Series III experiences a loss of supply power, turn off the power switch
for all components and do not restart the system until reliable power has been restored.
!
CAUTION: The IVIS Lumina XRMS Series III is sensitive, scientific equipment and
should not be moved by any user unless the system is located on the XWS-260
workstation. Due to the risk of potential damage, it is critical that only a trained
PerkinElmer technician moves the IVIS Lumina XRMS Series III. If it is necessary
to relocate the instrument, contact PerkinElmer Technical Support (see page 24).
See Appendix A on page 77 for more details on moving the IVIS Lumina XRMS
Series III on the XWS-260 workstation.
VOLTAGE! The IVIS Lumina XRMS Series III can operate at multiple voltages (100-
240 VAC); however, you are not permitted to change the input voltage to any of
the system components. Several internal modifications are required for voltage
change. If the operating voltage must be changed, contact PerkinElmer Technical
Support (see page 24).

IVIS®Lumina XRMS Series III Hardware Manual Chapter 2 | Important Safety Instructions 7
Overloading
Facilities should be adequately wired according to local building codes.
2.8 Servicing
Refer all servicing to PerkinElmer Technical Support (see page 24). If the IVIS Lumina XRMS
Series III is damaged and requires service, unplug the instrument from the outlet and contact
PerkinElmer Technical Support. Servicing by anyone other than an authorized PerkinElmer
representative voids the warranty covering the IVIS Lumina XRMS Series III.
2.9 Other Equipment
Use of any equipment other than that recommended by this manual has not been evaluated for safety
and, therefore, is the sole responsibility of the user.
Do not modify the IVIS Lumina XRMS Series III in ANY manner by making any kind of hole or
aperture in the instrument or removing any component of the radiation shielding.
!
WARNING! Do not overload wall outlets, extension cords, or integral convenience
receptacles as this can result in a risk of fire or electric shock. See Components
and Specifications on page 36 for more details on the power requirements of the
equipment.

3 Warnings
Electrical Safety
X-Ray Safety
Eye Safety and Burn Hazard on page 9
Mechanical Safety on page 9
Chemical and Biological Safety on page 10
Panels, Cover, and Modules on page 10
3.1 Electrical Safety
3.2 X-Ray Safety
VOLTAGE! DO NOT attempt to service the IVIS Lumina XRMS Series III yourself.
Although there are no voltages in excess of 24V inside the imaging chamber, local
line voltages (110VAC or 230VAC) are present in the lower electronics tray. The
light source module may be user-serviced for line fuse and lamp replacement
only. There are no other user serviceable electrical parts in the light source
module with the exception of the line fuse. See Fuse Replacement on page 65 for
instructions. Contact PerkinElmer Technical Support for other electrical service
needs (see page 24).
!
WARNING! If necessary, wipe exterior surfaces of the light source module with a
soft cloth. DO NOT use fluids to clean the exterior of the module. Do not allow
fluids of any kind to enter the light source module under any circumstances. See
Cleaning the IVIS Lumina XRMS Series III on page 69 for instructions.
!
WARNING! When the power is on, DO NOT disconnect or reattach the electrical
control cable that connects the fluorescence equipment (excitation filter
assembly) to the light source module and the IVIS Lumina XRMS Series III
(electronics tray). See System Components, page 45 for photographs of these
components. Disconnecting or reconnecting the control cable when the system
has electrical power will damage the system. Always turn off the switch on the
front panel of the light source module and the rear-mounted ON/OFF switch on
the IVIS Lumina XRMS Series III before you connect or disconnect any of these
cable connections.
!
WARNING! This equipment produces X-rays when energized.
!
WARNING! The IVIS Lumina XRMS Series III should be operated only by
personnel who have been trained in radiation safety and the operation
and safety instructions contained in this manual. PerkinElmer also
recommends that personnel who operate the equipment, or are close
proximity to the equipment, use a radiation film badge or other type of
appropriate personal dosimeter.

IVIS®Lumina XRMS Series III Hardware Manual Chapter 3 | Warnings 9
3.3 Eye Safety and Burn Hazard
The light source module and the connecting fiber optic cables produce intense light that can cause
eye damage. The module uses a tungsten halogen lamp bulb that operates at a high temperature,
which if exposed to a user's skin, could cause a burn.
\
3.4 Mechanical Safety
The imaging chamber of the IVIS Lumina XRMS Series III is heavy and weighs 160 lbs. (73 kg).
The IVIS Lumina XRMS Series III has many internal motorized components that can move at any
time. The imaging stage can move when the door is open. Care should be taken to keep hands and
equipment away from the sides of the platform when it is moving. Never place anything underneath
the platform.
Do not attempt to put anything into the lens opening of the camera as there are optical components
that can be compromised or damaged.
If the imaging chamber makes an unusual noise or appears to be jammed, turn off the power switch
located on the back of the instrument.
The X-ray scintillation plate is a delicate part and should not be handled with bare hands or sprayed
with cleaning agents.
\
!
WARNING! DO NOT operate the light source module or the fluorescence
equipment without all of the fiber optic cables connected at both of their end
connections.
!
WARNING! Do not attempt to replace the tungsten halogen lamp or lamp
assembly in the light source module. Lamp replacement requires PerkinElmer
Technical Support (see page 24) because access to the lamp could expose the
user to dangerous voltages and the IVIS Lumina XRMS Series III will require
recalibration after replacement of the halogen lamp or lamp assembly.
IMPORTANT: The IVIS Lumina XRMS Series III may only be moved on the XWS-260
Workstation. See Appendix A on page 77 for instructions.
!
WARNING! If the gas hoses become caught, kinked, or disconnected, do not
operate the instrument. Over exposure to anesthesia gas may occur.
!
CAUTION: DO NOT touch or expose the four diffusing reflectors and the exposed
emission filter to contaminants (see Figure 8.2 on page 59), as this may impair
imaging performance. The reflectors' surfaces have been surface treated for
optimum light diffusion.
!
CAUTION: The Living Image®software controls excitation filter selection. Do not
manually turn the numbered knob on the Excitation Filter Wheel Assembly (see
Figure 8.5 on page 60).

IVIS®Lumina XRMS Series III Hardware Manual Chapter 3 | Warnings 10
3.5 Chemical and Biological Safety
Normal operation may involve the use of test samples that are pathogenic, toxic, or radioactive. It is
your responsibility to ensure that all necessary safety precautions are taken before such materials are
used.
Dispose of all waste materials according to appropriate environmental health and safety guidelines.
It is your responsibility to decontaminate the IVIS Lumina XRMS Series III before requesting service
by PerkinElmer Technical Support. Ask your laboratory safety officer to advise you about the level
of containment required for your application and about the proper decontamination or sterilization
procedures to follow.
Handle all infectious samples according to good laboratory procedures and methods to prevent the
spread of disease.
3.6 Panels, Cover, and Modules
There are no user serviceable components in the lower electronics tray of the IVIS Lumina XRMS
Series III. Do not remove the electronics tray from the IVIS Lumina XRMS Series III or the cover
from the light source module unless you are instructed by and under the supervision of a PerkinElmer
technical service representative.
Do not modify the IVIS Lumina XRMS Series III in ANY manner by making any kind of hole or
aperture in the instrument or removing any component that is part of the radiation shielding.

4 Legal Notices
Introduction
Limited Warranty
Patents on page 12
Trademarks on page 13
Disclaimers on page 13
4.1 Introduction
This manual is provided to you by PerkinElmer Health Sciences, Inc. ("PEHS") on behalf of itself
and its affiliates, Caliper Life Sciences, Inc. ("Caliper") and Xenogen Corporation ("Xenogen").
PEHS, Caliper and Xenogen are referred to collectively throughout this manual as "PerkinElmer".
Section 4.2 below provides the standard limited warranty for the System and associated Living
Image®Software.
4.2 Limited Warranty
a. PerkinElmer provides the following limited warranty for each new IVIS®Lumina XRMS Series
III ("System") purchased from it as follows ("Limited Warranty"):
i. PerkinElmer warrants that This Limited Warranty for the System extends for a period of one
(1) year following delivery to, and installation of, the System to the original customer,
purchaser, or user ("Customer"), the System shall substantially conform to its published
specifications existing at the time of purchase. This Limited Warranty and is not assignable
or transferable to any successor, without the express written permission of PerkinElmer,
which may be withheld in its sole discretion.
ii. During the Limited Warranty period, PerkinElmer will repair or replace, at PerkinElmer's sole
option, any defective parts if such repair or replacement is needed because of System
malfunction or failure during normal usage in accordance with the instructions in this manual.
Repairs and replacements under the Limited Warranty will be made at PerkinElmer's expense.
Parts replaced during this Limited Warranty period may be retained by PerkinElmer, at its sole
option, and will be warranted for the longer of the remaining term of the original Limited
Warranty period, or for thirty (30) days from the date of replacement. PerkinElmer's limit of
liability under the Limited Warranty shall be the purchase price of the Imaging System.
PerkinElmer shall not be liable for any other losses or damages. These remedies are the
Customer's exclusive remedies for breach of warranty.
iii. No coverage or benefits shall be provided under this Limited Warranty if any of the following
conditions apply:
a) The System has been subjected to abnormal use, abnormal conditions, unauthorized
modifications (e.g., unauthorized installation of hardware or software), unauthorized
repair or servicing, misuse, neglect, abuse, accident, alteration, any use inconsistent with
or in contradiction to the instructions in this manual, or other acts which are not the fault
of PerkinElmer.
b) PerkinElmer was not advised in writing by the Customer of the alleged defect or
malfunction of the System within ten (10) days after the expiration of the applicable
limited warranty period.
iv. If a problem develops during the Limited Warranty period, the Customer shall contact
PerkinElmer Technical Support for assistance immediately following discovery of the
problem.

IVIS®Lumina XRMS Series III Hardware Manual Chapter 4 | Legal Notices 12
b. The following Limited Warranty provided by PerkinElmer is the sole and exclusive warranty
applicable to the Living Image®software ("Software"):
v. PerkinElmer warrants that such software will conform to PerkinElmer's program manuals
current at the time of shipment to Customer when properly installed, provided, however, that
PerkinElmer does not warrant that the operation of the software will be uninterrupted or error-
free.
vi. Customer agrees that as PerkinElmer's sole liability and as Customer's sole remedy,
PerkinElmer will provide services to correct documented and reproducible errors which
PerkinElmer's diagnosis indicates are caused by a defect in an unaltered version of the
Software.
c. THE FOREGOING LIMITED WARRANTY IS THE CUSTOMER'S SOLE AND EXCLUSIVE
REMEDY AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESS OR IMPLIED.
PERKINELMER SHALL NOT BE LIABLE FOR SPECIAL, INCIDENTAL OR
CONSEQUENTIAL DAMAGES, INCLUDING BUT NOT LIMITED TO LOSS OF
ANTICIPATED BENEFITS OR PROFITS, LOSS OF SAVINGS OR REVENUE, PUNITIVE
DAMAGES, LOSS OF USE OF THE SYSTEM OR ANY ASSOCIATED EQUIPMENT, COST
OF CAPITAL, COST OF ANY SUBSTITUTE EQUIPMENT OR FACILITIES, DOWNTIME,
THE CLAIMS OF ANY THIRD PARTIES, INCLUDING CUSTOMERS, AND INJURY TO
PROPERTY, RESULTING FROM THE PURCHASE OR USE OF THE SYSTEM OR ARISING
FROM BREACH OF THE WARRANTY, BREACH OF CONTRACT, NEGLIGENCE, STRICT
TORT, OR ANY OTHER LEGAL OR EQUITABLE THEORY, EVEN IF PERKINELMER
KNEW OF THE LIKELIHOOD OF SUCH DAMAGES. PERKINELMER SHALL NOT BE
LIABLE FOR DELAY IN RENDERING SERVICE UNDER THE LIMITED WARRANTY, OR
LOSS OF USE DURING THE PERIOD THAT THE SYSTEM IS BEING REPAIRED.
PERKINELMER DOES NOT REPRESENT OR WARRANT THAT THE EQUIPMENT OR
SOFTWARE WILL BE FREE FROM DEFECTS, UNINTERRUPTED OR, ERROR-FREE.
d. Some countries, states or provinces do not allow the exclusion or limitation of implied warranties
or the limitation of incidental or consequential damages for certain products or the limitation of
liability for personal injury, so the above limitations and exclusions may be limited in their
application to you. When any implied warranties are not allowed to be excluded in their entirety,
they will be limited to the duration of the applicable written warranty. This Limited Warranty
gives you specific legal rights which may vary depending on local law.
e. This Limited Warranty shall be governed by the laws of the Commonwealth of Massachusetts,
U.S.A., excluding its conflicts of laws principles and excluding the United Nations Convention
on Contracts for the International Sale of Goods.
4.3 Patents
The detection and imaging of light originating within mammals is the subject of several issued
patents and pending patent applications in the United States and around the world, including U.S.
Patent Numbers 5,650,135, 6,217,847, 6,649,143, 6,890,515, 6908605, 6916462, 6923951, 6939533,
7198774 and 7255851, and European Patent Commission Numbers EP0861093 and EP1016419, for
which Xenogen Corporation is the exclusive licensor. The use of an IVIS®Imaging System for such
applications requires a sublicense from Xenogen Corporation.
In addition, many of the hardware and software components of the Imaging System are the subject
of various issued patents and pending patent applications owned by Xenogen, including: United
States Patent Number 6,614,452 (Graphical User Interface for In Vivo Imaging); 6,775,567
(Improved Imaging Apparatus); 7113217 (Multi-view Imaging Systems), 7616985 (Method and
Apparatus for 3-D Reconstruction of Light Emitting Sources), 7403812 (Method and Apparatus for
Determining Target Depth, Brightness, and Size Within a Body Region), 6894289 (Fluorescence
illumination assembly for an imaging apparatus), and 6919919 (Light calibration device for use in
low level light imaging systems).

IVIS®Lumina XRMS Series III Hardware Manual Chapter 4 | Legal Notices 13
4.4 Trademarks
IVIS and Living Image are registered trademarks of PerkinElmer. The names of companies and
products mentioned herein may be the trademarks of their respective owners. Microsoft and
Windows are either registered trademarks or trademarks of Microsoft Corporation in the United
States and/or other countries. Pentium III is a registered trademark of Intel Corporation.
4.5 Disclaimers
a. Use of theSoftware. The Software and related documentation may be subject to clickwrap, shrink
wrap, or other end user license agreements containing restrictions on use and disclosure and are
protected by intellectual property laws. Except as expressly permitted in your license agreement
or allowed by law, you may not use, copy, reproduce, translate, broadcast, modify, license,
transmit, distribute, exhibit, perform, publish, or display any part, in any form, or by any means.
Reverse engineering, disassembly, or decompilation of this software, unless required by law for
interoperability, is expressly prohibited.
b. Use of the System. This manual has been developed for use by properly trained individuals only
and use by individuals who have not received proper training is not advised. Any changes or
modifications of the System, not expressly approved by PerkinElmer, will void the Limited
Warranty and any repair thereafter shall be charged to the Customer. Any movement of the
System not performed by authorized personnel of PerkinElmer may void the Limited Warranty.
Failure to operate the System in accordance with this manual is likely to cause safety hazards,
personal injury, property damage, and/or other damages.

5 X-ray Safety and Radiation Hazards
Introduction
Radiation Theory and X-ray Generation on page 14
Biological Effects of Radiation on page 16
IVIS Lumina XRMS Series III Safety Systems on page 17
Your Laboratory X-ray Safety Procedures on page 22
Contact Information on page 24
5.1 Introduction
Cabinet X-ray System
The IVIS®Lumina XRMS Series III produces X-rays when and only when the X-ray function has
been energized and initiated. The instrument may also be operated in standard bioluminescence or
fluorescence mode without X-ray generation. The IVIS Lumina XRMS Series III is defined by most
regulatory agencies as a "Cabinet X-Ray System." A cabinet system is one that produces little or no
X-ray exposure to the user and is safe to operate with the user in close proximity. This radiation is
confined to the interior of the imaging chamber. PerkinElmer certifies the IVIS Lumina XRMS Series
III to produce not more than 0.5 millirem per hour at a distance of 5 cm from the instrument surface.
In addition, the instrument is certified to meet all international exposure requirements (typically, 0.1
millirem per hour at 10 cm) and other regulations for where it is sold. The IVIS Lumina XRMS Series
III meets all US (FDA) regulations regarding a cabinet X-ray system. For information on
international limits for X-ray doses see X-ray Dose Limits on page 17 later in this section.
Other Product Documentation
Users will receive pre-installation information to help prepare the laboratory for installation of the
IVIS Lumina XRMS Series III. Included with this package of material is a document called 126198:
Safe Operating and Emergency Procedures for the operation of the IVIS Lumina XRMS Cabinet X-
ray System. This document includes a list of US and Canadian contacts for Radiation Protection.
Those contacts have been repeated in this manual for your convenience.
Only individuals who have been trained to operate the equipment should be permitted to use it. In
some locations, government regulations may require that the user have radiation training and be
certified.
5.2 Radiation Theory and X-ray Generation
Types of Radiation: Ionizing and Non-ionizing
Radiation is everywhere. Our bodies are continuously bathed in radiation in the form of sunlight,
radio and television waves as well as radiation produced by the earth's natural background radiation
produced by radioactivity and cosmic rays. Radiation is distinguished by its ability to ionize chemical
bonds, and is characterized as either ionizing or non-ionizing. Ionizing radiation has the ability to
affect biological organisms including human beings by interacting with the cellular chemistry.
Radiation such as sunlight, is usually considered non-ionizing radiation, although there is some