PFM Medical Rotary 3000 Compact User manual

Operating Manual
pfm Rotary 3000
Compact
Please read this operating manual
carefully before using the instrument

that you are familiar with the
method of operation as well as the
instrument’s controls to ensure that
it is used correctly.
www.pfmmedical.com
Quality and Experience

2pfm Rotary 3000 Compact Rev.2022-03-24
Note
Serial number:
Please enter the serial number of your instrument here.It can be found on the nameplate.
Dear Customer,


controls to ensure that it is used correctly.
All the information in this operating manual applies to the pfm Rotary 3000 Compact.
Note

3pfm Rotary 3000 Compact Rev.2022-03-24
Part 1 – Safety instructions 4
1.1 Key to symbols 4
1.2 Intended purpose 6
1.3 User group/user profile 6
1.4 Microtome safety devices 6
1.5 Operating the instrument 7
1.6 General precautions 7
Part 2 – Introduction 8
2.1 View of the pfm Rotary 3000 Compact 8
2.2 Technical data for the pfm Rotary 3000 Compact 8
2.3 Scope of delivery 8
Part 3 – Operating instructions 9
  
3.2 Assembling the accessories 10
3.3 Sectioning movement 14
  
3.5 Section counter 14
 
  
3.8 Trimming device/first incision 16
  
 
  
3.12 Universal cassette clamp 17
  
  
  
 
3.17 Knife holder 20
3.18 Knife holder base 20
 
3.20 Knife holder mounting device 21
3.21 Section waste tray 22
3.22 Malfunctions and their correction 22
Part 4 – Working with the microtome 23
  
4.2 Information about how to avoid problems 23
4.3 Troubleshooting 24
Part 5 – Maintenance, cleaning and care 25
5.1 Maintenance 25
5.2 Cleaning and care 25
5.3 Cleaning and care of touchscreen/display 25
5.4 Returning the microtome for repair or maintenance 26
  
5.6 Warranty 27
  
Part 6 – Disposal of the pfm Rotary 3000 Compact when put out of use 28
Part 7 – Accessories 29
Contents

4pfm Rotary 3000 Compact Rev.2022-03-24
Part 1 – Safety instructions


Always observe the following precautions during operation of the instrument. Failure to do so
is contrary to the recognised technical regulations and the intended use of the instrument.
If the above rule is violated, pfm medical ag shall assume no liability.
1.1 Key to symbols
Caution! This symbol indicates a possible danger to life and health.Failing to observe these

Indicates useful information
Indicates a dangerous situation
NOTE
WARNING
Manufacturer


IVD
Catalogue number
REF
Consult operating manual
NOTE The up-to-date electronic operating manual can be found on our website:
http://www.pfmmedical.com/downloads
Safety instructions

5pfm Rotary 3000 Compact Rev.2022-03-24
Safety instructions
Serial number

Biohazard warning
SN
The instrument must not be disposed of in normal domestic waste.
It must be collected separately.
Permissible temperature range at the place of use
+10°C - +40°C
Permissible temperature range for storage and transportation
-10°C - +50°C
Permissible humidity range for storage and transportation, and at the place of use

10°C
40°C
-10°C
50°C
10%
80%
Keep dry
Fragile, handle with care

6pfm Rotary 3000 Compact Rev.2022-03-24
1.2 Intended purpose
The pfm Rotary 3000 Compact is a compact,manual rotary
microtome designed to generate thin sections of human
and animal tissue samples of varying hardness, provided
the tissue is suitable for manual sectioning.The instrument
can be used for all applications in industry, research and
medicine as a diagnostic aid in connection with in vitro
examination as part of the histopathology process. It may

obtained using the device are intended to assist the pathologist
in evaluating the physiological or pathological condition of
the tissue samples.
1.3 User group/user profile
The pfm Rotary 3000 Compact may only be operated by

the method of operation of the instrument.

manual must be read so that the user is familiar with the
method of operation and the controls.This is essential to
ensure proper handling.
NOTE
The operating manual should be accessible to everyone.

1.4 Microtome safety devices
Finger guard on the knife holder



Handwheel lock


during the mounting process.
1
1
Finger guard
1

1
WARNING
Caution! Protective devices must not be removed from the instrument or modified. The proper functioning

Safety instructions

7pfm Rotary 3000 Compact Rev.2022-03-24





Select the correct blade holder for the microtome blade



position.The blade edge should always be pointing up.
The microtome blade should always be completely covered
by the pressure plate of the blade holder and not be
protruding.









When sectioning, always wear the necessary personal



holder from the instrument.For the blade holder,ensure

beforehand.



removed beforehand.
Observe all warnings.
Warnings on the instrument must not be removed or
obscured.
Only use suitable accessories recommended/supplied by
pfmmedical.
If unusual noises develop,please contact your responsible
pfm service technician or contact pfm medical ag directly.
The instrument may only be opened and repaired by service
technicians trained and authorised by pfmmedical.

the vicinity of the instrument, as they could cause corrosion
and damage the device and accessories.
To prevent slip hazards, laboratory shoes should be worn

microtome and surrounding area.
1.5 Operating the instrument

Risk of infection

safety measures must be implemented.The specimens used
may potentially be infectious.The general laboratory rules
regarding protection against infections must therefore be
observed.
Risk of radioactivity

protection regulations must be followed. Protective clothing
must be worn in accordance with the rules and regulations
that apply in your laboratory for handling radioactively
contaminated materials.For the disposal of radioactive waste,
the applicable regulations will apply in each instance.
Risk of chemical substances
Some of the alloys used in the components of this instrument
contain small amounts of lead for improved machining.The

used as intended,the lead in the alloys is not released and

precaution,we recommend that you wear gloves when
changing the accessory parts.


http://www.pfmmedical.com/downloads
1.6 General precautions
Safety instructions

8pfm Rotary 3000 Compact Rev.2022-03-24
Part 2 – Introduction
2.1 Views of the pfm Rotary 3000 Compact
Section counter display
Reset button
Coarse feed wheel

orientation with tangible zero-
point
Universal cassette clamp
Retraction system
Trimming device
Clamping lever for clearance angle


Handwheel


orientation
Finger guard
Pressure plate
Clamping lever for pressure plate
Knife holder
Section waste tray
2
7
3
4
5
8
9
13
14
15
16
17
1
6
11
12
5
9
3
4
7
1
10
8
2
6
12
10
13
14
11
15
16
17
Introduction
Coarse feed: manually via coarse feed wheel
Fine feed: manually via handwheel
Section thickness:
Setting range: 

Trimming: two trimming steps 10 and 20 µm
Retraction: 30 µm, can be switched off
 Horizontal range: 30 mm
2.2 Technical data for the pfm Rotary 3000 Compact
Vertical range: 60 mm
Object orientation: x- and y-axes universal 12°, z-axis up
to 360°
Maximum sample size:




Dimensions (W/D/H): 430 x 440 x 240 mm
Weight: 
2.3 Scope of delivery
pfm Rotary 3000 Compact
REF: 0300310
Scope of delivery
Base device
1 Section waste tray
1 Protective cover
Without blade holder base and disposable blade holder


NOTE
The up-to-date electronic operating manual can be found
on our website: http://www.pfmmedical.com/downloads

pfm Rotary 3000 Compact Rev.2022-03-24

complete and undamaged.






and transported to the installation location by at least two
people for safety reasons.

during transportation,the instrument should only be


Please read the operating manual carefully before using



set-up area.
The instrument should be placed on a stable,vibration-


surface.
Position the instrument so that the handwheel is freely
and easily accessible.
If other instruments in the vicinity of the microtome
generate vibrations,these can interfere with the sectioning
process.
Room temperature at the installation location of the
instrument between +10°C and +40°C.
Relative humidity at the installation location of the

Ambient temperature during the transportation and
storage of the instrument: between -10° and +50°C.
Relative humidity during the transportation and storage


responsibility for any use of special equipment and/or materials for operating the instrument.
NOTE

correctly if it needs to be sent for servicing.
3.1 Transportation, unpacking and setting up the instrument
Operating instructions
Part 3 – Operating instructions

10 pfm Rotary 3000 Compact Rev.2022-03-24
3.2 Assembling the accessories


Orientable adapter
1
2
3
1
3
2
Adapter orientable
Assembling the object clamps with/without adapter
Universal cassette clamp,
orientable
Universal cassette clamp,non-







NOTE


Operating instructions

11pfm Rotary 3000 Compact Rev.2022-03-24
Knife holder
2
2



Finger guard

Knife holder base
1
1
3
3
Assembling the knife holder base
2
Knife holder base
Knife holder base
Knife holder clamping levers
1
1
1
1
2
Guide rails
Knife holder mounting device
1




2
2
1
Operating instructions

12 pfm Rotary 3000 Compact Rev.2022-03-24
Assembling the disposable blade holder upper jaw
2
Pressure plate

Pressure plate clamping lever
Knife angle scale
1
1
2
3
3
4
4






Section waste tray
The section waste tray is pushed onto the microtome via
two guides on the inside and then positioned so that it is
felt to latch.
Operating instructions

13pfm Rotary 3000 Compact Rev.2022-03-24
Fitting the microtome blade
2
Finger guard
Pressure plate
Blade support
Pressure plate clamping lever
1
1
2
3
3
4
4

Release the clamping lever for the pressure plate.
Remove the microtome blade from the dispenser and carefully slide it under the pressure plate from the side.
It is easier to introduce the microtome blade by gently pressing on the pressure plate.This forms a small gap between


Clamp the pressure plate using the clamping lever.

Feather® microtome blade S35
Fitting a standard knife




screws at the side.


2
Finger guard
Screws
Knurled screw
1
3
1
3
2
Operating instructions

14 pfm Rotary 3000 Compact Rev.2022-03-24
Operating instructions
3.3 Sectioning movement
The sectioning movement of the pfm Rotary 3000 Compact is
controlled manually by turning the handwheel.The rotary


achieved by turning the handwheel further. Mechanical
retraction is activated during the upwards motion in order

Handwheel
1
1
3.4 Section thickness adjustment

1
1








3.5 Section counter
Section counter
1
1
A battery-operated section counter is used to add up the
sections performed.After each turn of the handwheel, the
counter increases by one.

15pfm Rotary 3000 Compact Rev.2022-03-24
3.6 Coarse adjustment of the object
1
Coarse feed wheel
1


to prevent any mechanical collision of components against the micrometer mechanism. In the front end area, the clutch



You can also release the clutch in the rear end area by pressing against the microtome and simultaneously turning it in

WARNING


2
Rear end position
Front end position
1
1
2
Operating instructions
3.7 Fine adjustment of the object
Handwheel
1
1



16 pfm Rotary 3000 Compact Rev.2022-03-24
3.9 Retraction
Operating instructions
Retraction on
1
1
3.8 Trimming device/first incision
1
Trimming device
1


for this purpose.The trimming device is positioned at the

the button pushed in the selected position.The two-step
system has a clear latching point and is easy to use even
when turning the handwheel.
WARNING

setting to the size of the sample.
1
Retraction
1
Retraction is activated during the upwards motion in order



1
1

17pfm Rotary 3000 Compact Rev.2022-03-24
3.10 Mounting the object


the sectioning level.The orientable adapter can be screwed
to the appropriate clamp if needed.
WARNING
Caution! It is imperative that the handwheel be

area.
Adapter orientable
1
1
3.11 Standard object clamp


WARNING


paraffin. Otherwise proper clamping cannot be
guaranteed.
3.12 Universal cassette clamp
The universal cassette clamp,which is operated via the
clamping lever, is used for mounting standard embedding
cassettes.The clamping lever must be pulled upward to
insert or withdraw an embedding cassette.
WARNING


paraffin. Otherwise proper clamping cannot be
guaranteed.
Operating instructions

18 pfm Rotary 3000 Compact Rev.2022-03-24
3.13 Object orientation


Movement along the x- and y-axes is possible using the

clamp position as soon as the required position is reached.

Zero-point setting


Clamping lever

1
2
1
Operating instructions
2
2


Unscrew the nut with a 13 mm open spanner.Then use an
8 mm open spanner to screw the threaded brass bolt
inwards or outwards as required and tighten the nut again
with the 13 mm spanner.
To test the clamping,insert the adapter in the guide bore
and tighten it with the clamping lever. If the clamping is
not adequate, the process must be repeated and the brass


directions.Adapter hole
Nut
Threaded brass bolt
1
1
3
3
2
3.14 Adjusting the orientable adapter

pfm Rotary 3000 Compact Rev.2022-03-24
3.15 Changing the object mounting device
Operating instructions




clamped. If it is not properly clamped,vibrations etc. can occur while sectioning.


Orientable adapter
1
2
3
1
3
2
Adapter orientable
3.16 Handwheel lock




WARNING
Caution! After completing the sectioning, the




Handwheel

1
1
2
2

20 pfm Rotary 3000 Compact Rev.2022-03-24
3.17 Knife holder
2
2




both sides is “only” clipped on and therefore removable.
WARNING
Caution! The finger guard must be used and the

holder. The position of the clamping lever on the

Finger guard

Knife holder base
1
1
3
3
3.18 Knife holder base
2



Knife holder base Knife holder base
Knife holder clamping levers
11
1
1
2
Operating instructions