PFM Medical Rotary 3006 EM User manual

Operating Manual
pfm Rotary 3006 EM
Please read this operating manual
carefully before using the instrument
for the first time and make certain
that you are familiar with the
method of operation as well as the
instrument’s operating elements to
ensure that it is used in the correct
way.
www.pfmmedical.com
Quality and Experience

2pfm Rotary 3006 EM Rev.2022-01-28
Serial number:
Please enter the serial number of your instrument here. It can be found on the nameplate.
Dear customer,

make certain that you are familiar with the method of operation as well as the instrument’s
controls to ensure that it is used correctly.
All the information in this operating manual applies to the pfm Rotary 3006 EM.
Note
Note

3pfm Rotary 3006 EM Rev.2022-01-28
Part 1 – Safety instructions 5
1.1 Key to symbols 5
1.2 Intended purpose 7
1.3 User group/user profile 7
1.4 Microtome safety devices 8
1.5 Operating the instrument 9
1.6 General precautions 10
Part 2 – Introduction 11
2.1 Views of the pfm Rotary 3006 EM 11
2.2 Technical data for the pfm Rotary 3006 EM 12
2.3 Technical data control panel 12
2.4 Scope of delivery 13
Part 3 – Operating instructions 14
3.1 Transportation, unpacking and setting up 14
3.2 Assembling the accessories 15
3.3 Initial operation 20
3.4 Switching on the instrument 20
3.5 Operating controls 20
3.6 Operating display on the instrument/control panel 21
3.7 Control panel 21
3.8 Menu settings 22
3.9 Selecting Trimming and Coarse Feed modes 22
3.10 Function and adjustment of the Trimming and Coarse Feed modes 22
3.11 Setting the section thickness 23
3.12 Retraction of sample 23
3.13 End position 23
3.14 Sectioning speed 23
3.15 Setting the sectioning speed 23
3.16 Sectioning modes 24
3.17 Starting/stopping the sectioning process (motor-driven) 24
3.18 Sectioning the sample manually/motor-driven 25
3.19 Changing samples/interruption of work 25
3.20 Memo 25
3.21 Remaining feed indication 26
3.22 Section counter/addition 26
3.23 Mounting the object 26
3.24 Standard object clamp 26
3.25 Universal cassette clamp 27
3.26 Object orientation 27
3.27 Adjusting the orientable adapter 27
3.28 Setting the clearance angle 28
3.29 Connecting the foot switch (optional accessories) 28
3.30 Removing/attaching the object mounting device 28
3.31 Handwheel lock 29
3.32 Knife holder 29
3.33 Knife holder base 29
Content

4pfm Rotary 3006 EM Rev.2022-01-28
Content
3.34 Knife holder mounting device 30
3.35 Disposable blade holder upper jaw low profile (narrow blade) 30
3.36 Section waste tray 31
3.37 Malfunctions and their correction 31
Part 4 – Working with the microtome 32
4.1 Preparing, embedding and sectioning the object 32
4.2 Information about how to avoid problems 32
4.3 Trouble-shooting 33
Part 5 – Microtome maintenance, cleaning and care 34
5.1 Maintenance 34
5.2 Cleaning and care 34
5.3 Cleaning and care touch screen/display 34
5.4 Returning the microtome for repair or maintenance 35
5.5 Decontamination 35
5.6 Instrument fuse 35
5.7 Warranty 36
5.8 Declaration of Conformity 36
Part 6 – Disposal of the microtome when put out of use 37
Part 7 – Accessories 38

5pfm Rotary 3006 EM Rev.2022-01-28
Part 1 – Safety instructions
Please read this operating manual carefully before working with the pfm Rotary 3006 EM.
Make certain that you are familiar with the instrument’s controls and the method of operation.
Please observe the following precautions during each operation of the instrument.
Failure to observe these precautions is contrary to the recognised standards of good practice
and the intended use of the instrument.
In case of infringement, pfm medical ag does not accept any form of liability.
1.1 Overview of symbols and their meanings
Caution! This symbol indicates a possible danger to life and health.
Failing to observe these instructions can result in severe health impacts, including
life-threatening injuries.
Indicates useful information
Indicates a dangerous situation
NOTE
WARNING
Manufacturer
Date of manufacture
In vitro diagnostic (IVD) medical device
IVD
Catalogue number
REF
Safety instructions
Consult operating manual
NOTE The current electronic operating manual can be found on our website:
http://www.pfmmedical.com/downloads

6pfm Rotary 3006 EM Rev.2022-01-28
Serial number
CE marking
Biohazard warning
SN
The instrument must not be disposed of in normal domestic waste.
It must be collected separately.
Permissible temperature range at the place of use
+10°C - +40°C
Permissible temperature range for storage and transportation
-10°C - +50°C
Permissible humidity range for storage and transportation, and at the place of use
10% - 80% (non-condensing)
10°C
40°C
-10°C
50°C
10%
80%
Safety instructions
Keep dry
Fragile, handle with care

7pfm Rotary 3006 EM Rev.2022-01-28
1.2 Intended purpose
The pfm Rotary 3006 EM is a modern, fully electronic
rotary microtome designed to generate thin sections of
human and animal tissue samples of varying hardness,
provided the tissue is suitable for sectioning. The
instrument can be used for all applications in industry,
research and medicine as a diagnostic aid in connection
with in vitro examination as part of the histopathology
process. It may only be operated by appropriately trained

to assist the pathologist in evaluating the physiological or
pathological condition of the tissue samples.
1.3 User group/user prole
The pfm Rotary 3006 EM may only be operated by

the method of operation of the instrument.

operating manual must be read so that the user is familiar
with the method of operation and the control elements.
Only in this way can proper handling be ensured.
NOTE
The operating manual should be accessible to everyone.
Safety instructions

1.4 Microtome safety devices
Finger guard on the knife holder
Before manipulating the knife, knife holder or object, or
changing the object, always cover the blade edge with the

Handwheel lock
When the object and knife are being mounted, the
handwheel lock should be activated to prevent injury
during the mounting process. The M-STOP
when the handwheel is locked.
1
1
Finger guard
1
M-STOP
Handwheel lock
1

8pfm Rotary 3006 EM Rev.2022-01-28
Emergency Stop function
The E-STOP
button is pressed. The emergency stop function can be
deactivated by turning the Emergency Stop push button in
the direction indicated by the arrow. This must be followed
by a reference run (press Start).
1
Emergency Stop push button
1
E-STOP
Removable handle
The handle can be removed from the side by pressing
the button on the front.
1
1
Handle button
Handle
1
1
WARNING
Caution! For safety reasons, the handle should
always be removed for motor-driven sectioning.
WARNING
Caution! Safety devices must not be removed from the instrument or changed. The proper
functioning of the safety devices must be checked by the user before starting work.
Safety instructions

9pfm Rotary 3006 EM Rev.2022-01-28
Take care when handling knives and microtome blades;
the blade edge is very sharp and may cause severe injury.
When setting up the sample and the knife/blade holder,
the handwheel lock should be activated to prevent

guard.
Select the correct blade holder for the microtome blade

When setting up the knife/microtome blade in the blade
holder, make sure that the blade edge is in the correct
position. The blade edge should always be pointing up.
The microtome blade should always be completely covered
by the pressure plate of the blade holder and not be
protruding.

the knife/blade holder into the knife holder.

the sample and the knife/microtome blade.


Never try to catch a falling knife/blade holder.
Take care during sectioning and when removing sections;
the blade edge is very sharp and may cause severe injury.
When sectioning, always wear the necessary personal
protective equipment (PPE) (safety work shoes/protective
gloves/cut-resistant gloves/face mask/protective goggles).
After completing the work, always remove the knife/
blade holder from the instrument For the blade holder,
ensure that the knife/microtome blade has been removed
beforehand.
A knife/blade holder that is not in use should always be
kept in a safe place (e.g. a knife box) for safety reasons.
For the blade holder, ensure that the knife/microtome
blade is removed beforehand.
Observe the warnings.
Warnings on the instrument must not be removed or
obscured.
Only use suitable accessories and connecting cables
recommended/supplied by pfmmedical.
If the instrument fuse needs to be replaced, the

(T 2.0A).
Before starting the reference run always move the blade
holder to the front position. Otherwise the blade holder
may be damaged, because, depending on the position of
the object holder, the latter moves downward during the
reference run.
If unusual noises develop, please contact your responsible
pfm service technician or contact pfm medical ag
directly.
The instrument may only be opened and repaired
by service technicians trained and authorised by
pfmmedical.
No corrosive, acidic or alkaline solutions should be used
in the vicinity of the instrument. These could cause
corrosion on the instrument and accessories, and damage
them.
To prevent slip hazards, laboratory shoes should be worn

microtome and surrounding area.
1.5 Operating the instrument
Safety instructions

10 pfm Rotary 3006 EM Rev.2022-01-28
Please take special note of the following warnings:
Beware of explosive gases
The instrument may not be used if dangerous gas
concentrations could occur in the surroundings.
Risk of infection
When working with infectious materials, appropriate
safety measures must be implemented. The samples used
may potentially be infectious. The general laboratory rules
regarding protection against infections must therefore be
observed.
Risk of radioactivity
When working with radioactive materials, the radiation
protection regulations must be followed. Protective
clothing must be worn in accordance with the rules and
regulations that apply in the respective laboratory for
handling radioactively contaminated materials. For the
disposal of radioactive waste, the applicable regulations
will apply in each instance.
Risk of chemical substances
For better cutting properties, some of the component
alloys of this instrument contain small amounts of lead,

intended, the lead in the alloys is not released and does not
represent any risk to the user. As an additional precaution,
we recommend that you wear gloves when changing the

Declaration on the EU Regulation 1907/2006 (REACH) at:
http://www.pfmmedical.com/downolads
Avoiding malfunctions
To avoid malfunctions the instrument may not be used
close to radio transmitters, e.g. mobile phones. If the

your distributor.
Caution

please check at the installation location that the supply
voltage/frequency match the information given on the
nameplate.
Protective earthing of the instrument
In order to rule out the risk of electrocution, the
instrument must be connected to the earth conductor.
The instrument is equipped with a mains power plug
with earth conductor. It is very important to check that
the mains power outlet used is equipped with an earth
conductor, and that it complies with the regulations of the
International Electrotechnical Commission (IEC).
Mains voltage precautions
Never remove housing components. The replacement of
components and alignment must only be carried out by
trained service technicians.
Interference due to electromagnetic interactions
The electromagnetic environment should be assessed before
the microtome is operated. Do not use this instrument near
strong sources of electromagnetic radiation (for example,
unshielded, intentionally operated high-frequency sources)
because these can interfere with the proper operation

requirements of the standard IEC 61326-2-6 on immunity
and emissions.
1.6 General safety precautions
WARNING
Caution! Switch off the instrument and pull out
the mains plug before removing any part of the
housing.
Safety instructions

11pfm Rotary 3006 EM Rev.2022-01-28
Part 2 – Introduction
2.1 Views of the pfm Rotary 3006 EM
Display
Control wheel Settings
Control wheel Coarse
Feed/Trimming
Control wheel
Sectioning Speed
Emergency Stop
push button
Clamping lever for
clearance angle
adjustment
Clamping levers for
knife holder
Clamping lever for
object orientation
Handle button
Section waste tray
Finger guard
Pressure plate
Knife holder
Handwheel
Adjustment screws for
object orientation with
tangible zero-point
Handwheel lock
Clamping lever for
pressure plate
Knife angle scale
2
7
16
16
3
4
5
8
11
17
17
9
10
13
14
14
12
18
18
1
6
10
15
15
9
8
2
7
3
4
5
1
6
12
13
11
Introduction

12 pfm Rotary 3006 EM Rev.2022-01-28
Nameplate
Foot switch connection
Mains connection/
instrument fuse
Control panel connection
2
3
3
1
1
2
4
4
Section thickness:
Setting range:


Trimming mode:
Coarse feed speed: 1.0 mm/sec.
Sectioning modes: manual, continuous, single and step
Sectioning speed max.: 300 mm/sec.
Retraction:
operation)
Remaining feed indication 1 mm horizontal: visual and
audible
Horizontal range: 30 mm
Vertical range: 70 mm
Object orientation: x- and y-axes universal 12° z-axis up
to 360°
2.2 Technical data for the pfm Rotary 3006 EM
Maximum sample size:



cassettes
Power supply: 100/120/230/240 V AC, 50 - 60 Hz
Operating environment altitude: up to 2,000 m above
sea level
Pollution degree (according to IEC 61010-1): II
Overvoltage category (according to IEC 61010-1): II
Fluctuations in mains voltage: ±10% of the mains
voltage
IP protection class: IP20
Dimensions (W/D/H): 450 x 530 x 275 mm
Weight: 38 kg
2.3 Technical data control panel
Dimensions (W/D/H): 135 x 170 x 55 mm
Height opened: 150 mm
Weight: 1.9 kg
Introduction

13pfm Rotary 3006 EM Rev.2022-01-28
2.4 Scope of delivery
pfm Rotary 3006 EM
REF: 0300610
Scope of delivery
Base device
1 section waste tray
1 mains cable
1 protective cover
Without blade holder base and disposable blade holder upper jaw
Without object clamp and adapter
Without foot switch
Without control panel
NOTE
The current electronic operating manual can be found on our
website: http://www.pfmmedical.com/downloads
Introduction

14 pfm Rotary 3006 EM Rev.2022-01-28
Part 3 – Operating instructions
Before unpacking the delivery, please check that it is
complete and undamaged.
Disassemble the packaging and remove the instrument
and accessories from the packaging. The section waste
tray is included in the instrument packaging, but the
other accessories are packaged separately.
Take care when unpacking the instrument. The
instrument is very heavy and should be removed from the
packaging and transported to the installation location by
at least two people for safety reasons.
When lifting the instrument out of the packaging and
during transportation, the instrument should only be
handled at the base plate. Do not carry of lift the
instrument at the handwheel or object mounting device.
Please read the operating manual carefully before using

When setting up or moving the instrument, make sure

set-up area.
The instrument should be positioned on a stable,
vibration-free table.
The instrument must only be positioned on non-

Position the instrument so that the handwheel is freely
and easily accessible.
If other instrument in the vicinity of the microtome
generates vibrations, these can interfere with
the sectioning process.
Position the instrument so that the power plug is always
accessible.
Ambient temperature at the installation location of the
instrument between + 10°C and + 40°C.
Relative humidity at the installation location of the
instrument max. 80% (non-condensing).
Ambient temperature during the transportation and
storage of the instrument: between -10° and +50°C.
Relative humidity during the transportation and
storage of the instrument: max. 80% (non-condensing).
The operator is responsible for checking that the instrument is working properly and safely.
The operator shall assume responsibility for any use of special instrument and materials for operating the instrument.
NOTE
If possible, retain the original packaging for the entire lifetime of the instrument so that,
if it requires servicing, you can package the instrument correctly again.
3.1 Transportation, unpacking and setting up the instrument
Operating instructions

15pfm Rotary 3006 EM Rev.2022-01-28
3.2 Assembling the accessories
Clamping lever for object orientation
Adjusting screws for object orientation
Adapter orientable
1
2
3
1
3
2
Adapter orientable
Assembling the object clamps with/without adapter
Universal cassette clamp, orientable Universal cassette clamp, non-orientable (without adapter)
Release and remove the clamping lever for object orientation.
Turn the two adjustment screws for object orientation to the tangible zero-point (marked in red).
Insert the object clamp with the adapter, paying attention to the position of the hole so that the

Clamp the object clamp using the clamping lever.

NOTE
Some object clamps are optionally also available as a non-orientable version. For use on the instrument, the object clamp

Operating instructions

16 pfm Rotary 3006 EM Rev.2022-01-28
Knife holder
2
2
The knife holder consists of the knife holder base and


Finger guard
Disposable blade holder upper jaw
Knife holder base
1
1
3
3
Assembling the knife holder base
2
Knife holder base
Knife holder base
Knife holder clamping levers
1
1
1
1
2
Guide rails
Knife holder mounting device
1
Release the knife holder clamping levers.

Clamp the knife holder mounting device using the knife holder clamping levers.

2
2
1
Operating instructions

17pfm Rotary 3006 EM Rev.2022-01-28
Assembling the disposable blade holder upper jaw
2
Pressure plate
Clearance angle adjustment clamping lever
Pressure plate clamping lever
Knife angle scale
1
1
2
3
3
4
4
Release and completely remove the clearance angle adjustment clamping lever.
Insert the adapter on the disposable blade holder upper jaw into the knife holder base. Take note of the position of the

Set the clearance angle to 10° using the knife angle scale.
Clamp the clearance angle adjustment clamping lever.

Connecting the foot switch
1
Foot switch connection
1
The colour-marked (blue) connection for the foot switch is located on the back of the instrument.
Connect the foot switch cable to the foot switch socket.
WARNING
Caution! Please observe the guide on the foot switch socket and do not turn the plug in the
connection socket.
Foot switch
Operating instructions

18 pfm Rotary 3006 EM Rev.2022-01-28
Connecting the control panel
Control panel connection
1Control panel
The colour-marked (green) connection for the control panel is located on the back of the instrument.
Connect the control panel cable to the control panel socket.
WARNING
Caution! Please observe the guide on the foot switch socket and do not turn the plug in the
connection socket.
Section waste tray
1
Operating instructionsOperating instructions
The section waste tray is pushed onto the microtome via
two guides on the inside and then positioned so that it is
felt to latch.

19pfm Rotary 3006 EM Rev.2022-01-28
Fitting the microtome blade
2
Finger guard
Pressure plate
Blade support
Pressure plate clamping lever
1
1
2
3
3
4
4

Release the clamping lever for the pressure plate.
Remove the microtome blade from the dispenser and carefully slide it under the pressure plate from the side.
It is easier to introduce the microtome blade by gently pressing on the pressure plate. This forms a small gap between
the blade support and the pressure plate, making it easier to introduce and position the microtome blade.
Make sure that the microtome blade is positioned parallel to the pressure plate.
Clamp the pressure plate using the clamping lever.

Fitting a standard knife
Feather® microtome blade S35

Loosen the two screws on the standard knife holder.
Insert the knife into the knife holder from the side.

at the side.
Clamp the knife using the two screws.

2
Finger guard
Screws
Knurled screw
1
3
1
3
2
Operating instructions

20 pfm Rotary 3006 EM Rev.2022-01-28

must make sure that the supply voltage and frequency


Only connect the instrument to earthed wall sockets.
Connect the mains power cable to the connection socket
at the back of the instrument.
The insert for the instrument fuse (T2.0A) is situated next
to the connection socket.
Now switch on the instrument using the power switch.
1=ON, 0=OFF
WARNING
Caution! Only use the supplied mains power
cable with a ferrite core.
Caution! Do not operate the instrument if the
mains power cable is damaged.
Caution! Do not use an extension cable for the
connection.
Caution! If the instrument is not used with the
supplied mains power cable, the user must carry
out a safety test with the new mains power cable
before the instrument is used for the first time.
After switching on the instrument an audible signal can
be heard, and “REFERENCING” is shown on the display The
reference run is started by pressing START .After this,
the display automatically goes back to the control panel
display.
WARNING
Caution! Before starting the reference run
always move the knife holder to the front
position. Otherwise the knife holder may be
damaged, because, depending on the position of
the object holder, the latter moves downward
during the reference run.
Caution! Do not operate the instrument if the
display has no function, shows illogical or
incorrect values or is damaged.
3.3 Initial operation
3.4 Switching on the instrument
Connection socket
instrument fuse
Power switch
1
2
3
12 3
3.5 Operating controls
The pfm Rotary 3006 EM is equipped with a modern
touch screen system. The control panels and displays
are clear and arranged by function. The user can operate
the instrument either via the operating display on the
instrument or via a separate control panel.
This allows for simple and comfortable operation. When
the control panel is connected, the instrument can only
be controlled from the control panel. The control panel is
not included in the general scope of delivery and must be
ordered separately.
Operating instructions