PFM Medical Rotary 3005 User manual

Operating Manual
pfm Rotary 3005 E
Please read this operating manual
carefully before using the instrument
for the first time and make certain
that you are familiar with the method
of operation as well as the instrment’s
operating elements to ensure that it
is used in the correct way.
www.pfmmedical.com
Quality and Experience

2pfm Rotary 3005 E Rev.2022-03-21
Serial number:
Please enter the serial number of your instrument here.It can be found on the nameplate.
Dear Customer,

and make certain that you are familiar with the method of operation as well as the
instrument’s operating elements to ensure that it is used in the correct way.
All the information in this operating manual applies to the pfm Rotary 3005 E.
Note
Note

3pfm Rotary 3005 E Rev.2022-03-21
Part 1 – Safety instructions 5
1.1 Key to symbols 5
1.2 Intended purpose 7
1.3 User group/user profile 7
1.4 Microtome safety devices 7
1.5 Operating the instrument 8
1.6 General precautions 9
Part 2 – Introduction 10
2.1 Views of the pfm Rotary 3005 E 10
2.2 Technical data for the pfm Rotary 3005 E 11
2.3 Technical data control panel 11
2.4 Scope of delivery 12
Part 3 – Operating instructions 13
3.1 Transportation, unpacking and setting up 13
3.2 Assembling the accessories 14
3.3 Initial operation 18
3.4 Switching on the instrument 19
3.5 Operating controls 19
3.6 Operating display on the instrument/control panel 19
3.7 Control panel 20
3.8 Menu settings 20
3.9 Selecting Trimming and Coarse Feed modes 20
3.10 Function and adjustment of the Trimming and Coarse Feed modes 21
3.11 Setting the section thickness 21
3.12 Retraction of sample 21
3.13 Sectioning the sample 21
3.14 Changing samples/interruption of work 22
3.15 Memo 22
3.16 Remaining feed indication 22
3.17 Section counter 22
3.18 Mounting the object 23
3.19 Standard object clamp 23
3.20 Universal cassette clamp 23
3.21 Object orientation 24
3.22 Adjusting the orientable adapter 24
3.23 Setting the clearance angle 24
3.24 Removing/attaching the object mounting device 25
3.25 Handwheel lock 25
3.26 Knife holder 26
3.27 Knife holder base 26
3.28 Knife holder mounting device 27
3.29 Disposable blade holder upper jaw low profile (narrow blade) 27
3.30 Section waste tray 28
3.31 Malfunctions and their correction 28
Contents

4pfm Rotary 3005 E Rev.2022-03-21
Contents
Part 4 – Working with the microtome 29
4.1 Preparing, embedding and sectioning the object 29
4.2 Information about how to avoid problems 29
4.3 Trouble-shooting 30
Part 5 – Microtome maintenance, cleaning and care 31
5.1 Maintenance 31
5.2 Cleaning and care 31
5.3 Cleaning and care of touchscreen/display 31
5.4 Returning the microtome for repair or maintenance 32
5.5 Decontamination 32
5.6 Instrument fuse 32
5.7 Warranty 33
5.8 Declaration of Conformity 33
Part 6 – Disposal of the pfm Rotary 3005 E when put out of use 34
Part 7 – Accessories 35

5pfm Rotary 3005 E Rev.2022-03-21
Safety instructions
Part 1 – Safety instructions
Please read this operating manual carefully before working with the pfm Rotary 3005 E. Make
certain that you are familiar with the instrument‘s controls and method of operation.Always
observe the following precautions during operation of the instrument.Failure to do so is contrary
to the recognised technical regulations and the intended use of the instrument.
If the above rule is violated, pfm medical ag shall assume no liability.
1.1 Key to symbols
Caution! This symbol indicates a possible danger to life and health.Failing to observe these
instructions can result in severe health impacts, including life-threatening injuries.
Indicates useful information
Indicates a dangerous situation
NOTE
WARNING
Manufacturer
Date of manufacture
In vitro diagnostic (IVD) medical device
IVD
Catalogue number
REF
Consult operating manual
NOTE The up-to-date electronic operating manual can be found on our website:
http://www.pfmmedical.com/downloads

6pfm Rotary 3005 E Rev.2022-03-21
Safety instructions
Serial number
CE marking
Biohazard warning
SN
The instrument must not be disposed of in normal domestic waste.
It must be collected separately.
Permissible temperature range at the place of use
+10°C - +40°C
Permissible temperature range for storage and transportation
-10°C - +50°C
Permissible humidity range for storage and transportation, and at the place of use
10% - 80% (non-condensing)
10°C
40°C
-10°C
50°C
10%
80%
Keep dry
Fragile, handle with care

7pfm Rotary 3005 E Rev.2022-03-21
1.2 Intended purpose
The pfm Rotary 3005 E is a modern,semi-electronic rotary
microtome designed to generate thin sections of human
and animal tissue samples of varying hardness, provided
the tissue is suitable for manual sectioning.The instrument
can be used for all applications in industry, research and
medicine as a diagnostic aid in connection with in vitro
examination as part of the histopathology process. It may

obtained using the device are intended to assist the
pathologist in evaluating the physiological or pathological
condition of the tissue samples.
1.3 User group/user profile
The pfm Rotary 3005 E may only be operated by

the method of operation of the instrument.

operating manual must be read so that the user is familiar
with the method of operation and the operating controls.
This is essential to ensure proper handling.
NOTE
The operating manual should be accessible to everyone.
Safety instructions

1.4 Microtome safety devices
Finger guard on the knife holder
Before manipulating the knife, knife holder or object, or
changing the object, always cover the blade edge with the

Handwheel lock
When the object and knife are being mounted, the
handwheel lock should be activated to prevent injury
during the mounting process.The M-STOP
up when the handwheel is locked.
1
1
Finger guard
1
M-STOP
Handwheel lock
1
WARNING
Caution! Protective devices must not be removed from the instrument or modified. The proper
functioning of the protective devices must be checked by the user before starting work.

8pfm Rotary 3005 E Rev.2022-03-21
Safety instructions
Take care when handling knives and microtome blades;
the blade edge is very sharp and may cause severe injury.
When setting up the sample and the knife/blade holder,
the handwheel lock should be activated to prevent
injuries.The blade edge should be covered with the

Select the correct blade holder for the microtome blade

When setting up the knife/microtome blade in the blade
holder, make sure that the blade edge is in the correct
position.The blade edge should always be pointing up.
The microtome blade should always be completely covered
by the pressure plate of the blade holder and not be
protruding.

knife/blade holder into the knife holder.

the sample and the knife/microtome blade.


Never try to catch a falling knife/blade holder.
Take care during sectioning and when removing sections;
the blade edge is very sharp and may cause severe injury.
When sectioning, always wear the necessary personal
protective equipment (PPE) (safety work shoes/protective
gloves/cut-resistant gloves/face mask/protective goggles).
After completing the work, always remove the knife/blade
holder from the instrument.For the blade holder, ensure
that the knife/microtome blade has been removed
beforehand.
A knife/blade holder that is not in use should always be
kept in a safe place (e.g. a knife box) for safety reasons. For
the blade holder, ensure that the knife/microtome blade is
removed beforehand.
Observe all warnings.
Warnings on the instrument must not be removed or
obscured.
Only use suitable accessories and connecting cables
recommended/supplied by pfmmedical.
If the instrument fuse needs to be replaced, the

(T 2.0A).
Before starting the reference run always move the blade
holder to the front position. Otherwise the blade holder
may be damaged, because,depending on the position of
the object holder, the latter moves downward during the
reference run.
If unusual noises develop,please contact your responsible
pfm service technician or contact pfm medical ag directly.
The instrument may only be opened and repaired by
service technicians trained and authorised by pfmmedical.
No corrosive, acidic or alkaline solutions should be used in
the vicinity of the instrument, as they could cause
corrosion and damage the device and accessories.
To prevent slip hazards, laboratory shoes should be worn

microtome and surrounding area.
1.5 Operating the instrument

9pfm Rotary 3005 E Rev.2022-03-21
Safety instructions
Please take special note of the following warnings:
Beware of explosive gases
The instrument must not be used if dangerous gas
concentrations could occur in the surrounding area.
Risk of infection
When working with infectious materials,appropriate
safety measures must be implemented.The samples used
may potentially be infectious.The general laboratory rules
regarding protection against infections must therefore be
observed.
Risk of radioactivity
When working with radioactive materials, the radiation
protection regulations must be followed. Protective clothing
must be worn in accordance with the rules and regulations
that apply in your laboratory for handling radioactively
contaminated materials.For the disposal of radioactive waste,
the applicable regulations will apply in each instance.
Risk of chemical substances
Some of the alloys used in the components of this instrument
contain small amounts of lead for improved machining.The

used as intended,the lead in the alloys is not released and
does not represent any risk to the user. As an additional
precaution,we recommend that you wear gloves when

information in the Declaration on the EU Regulation
1907/2006 (REACH) at:
http://www.pfmmedical.com/downloads
Avoiding malfunctions
To avoid malfunctions, the instrument must not be used
close to radio transmitters, e.g. mobile phones. If the

your dealer.
Caution

please check that the supply voltage/frequency at the place

Protective earthing of the instrument
To avoid the risk of electrocution, the instrument must be
properly connected to an earth conductor.The instrument
is equipped with a mains power plug with earth conductor.
It is very important to check that the mains power outlet
used is equipped with an earth conductor, and that it
complies with the regulations of the International
Electrotechnical Commission (IEC).
Mains voltage precautions
Never remove any part of the housing.The replacement of
components and alignment must only be carried out by
trained service technicians.
Electromagnetic interference
The electromagnetic environment should be assessed before
the microtome is operated. Do not use this instrument near
strong sources of electromagnetic radiation (for example,
unshielded, intentionally operated high-frequency sources)
because these can interfere with the proper operation of the

of the standard IEC 61326-2-6 on immunity and emissions.
1.6 General precautions
WARNING
Caution! Switch off the instrument and pull out
the mains plug before removing any part of the
housing.

10 pfm Rotary 3005 E Rev.2022-03-21
Part 2 – Introduction
2.1 Views of the pfm Rotary 3005 E
Control wheel Settings
Control wheel Coarse
Feed/Trimming
Display
Universal cassette clamp
Clamping lever for
clearance angle
adjustment
Clamping levers for knife
holder
Clamping lever for object
orientation
Finger guard
Pressure plate
Knife holder
Section waste tray
Handwheel
Adjustment screws for
object orientation with
tangible zero-point
Handwheel lock
Clamping lever for
pressure plate
Knife angle scale
2
7
16
14
3
4
5
8
11
15
9
8
13
14
12
16
1
6
10
15
13
12
7
1
6
2
3
4
5
9
10
11
Introduction

11pfm Rotary 3005 E Rev.2022-03-21
Nameplate
Mains connection/
instrument fuse
Foot switch connection
2
1
1
2
3
3
Section thickness:
Setting range:


 Trimming mode:
 Retraction:
(in manual operation)
 Coarse feed speed: 1.0 mm/sec.
Remaining feed indication 1 mm horizontal: visual and
audible
Horizontal range: 30 mm
Vertical range: 70 mm
Object orientation: x- and y-axes universal 12° z-axis up
to 360°
Maximum sample size:



cassettes
2.2 Technical data for the pfm Rotary 3005 E
Power supply: 100/120/230/240 V AC,50 - 60 Hz
Operating environment altitude: up to 2,000 m above
sea level
Pollution degree (according to IEC 61010-1): II
Overvoltage category (according to IEC 61010-1): II
Fluctuations in mains voltage: ±10% of the mains
voltage
IP protection class: IP20
Dimensions (W/D/H): 450 x 530 x 275 mm
Weight: 38 kg
2.3 Technical data control panel
Dimensions (W/D/H): 135 x 170 x 55 mm
Height opened: 150 mm
Weight: 1.9 kg
Introduction

12 pfm Rotary 3005 E Rev.2022-03-21
2.4 Scope of delivery
pfm Rotary 3005 E
REF: 0300510
Scope of delivery
Base device
1 Section waste tray
1 Mains power cable
1 Protective cover
Without blade holder base and disposable blade holder
upper jaw
Without object clamp and adapter
Without control panel
NOTE
The up-to-date electronic operating manual can be found
on our website: http://www.pfmmedical.com/downloads
Introduction

13pfm Rotary 3005 E Rev.2022-03-21
Part 3 – Operating instructions
Before unpacking the delivery, please check that it is
complete and undamaged.
Disassemble the packaging and remove the instrument
and accessories from the packaging.The section waste
tray is included in the instrument packaging, but the
other accessories are packaged separately.
Take care when unpacking the instrument.The instrument
is very heavy and should be removed from the packaging
and transported to the installation location by at least two
people for safety reasons.
When lifting the instrument out of the packaging and
during transportation, the instrument should only be
handled at the base plate.Do not carry of lift the instrument
at the handwheel or object mounting device.
Please read the operating manual carefully before using

When setting up or moving the instrument, make sure

set-up area.
The instrument should be placed on a stable,vibration-
free table or desk.

surface.
Position the instrument so that the handwheel is freely
and easily accessible.
If other instruments in the vicinity of the microtome
generates vibrations,these can interfere with the sectioning
process.
Position the instrument so that the power plug is always
accessible.
Room temperature between + 10°C and + 40°C.
Relative humidity at the installation location of the
instrument max.80% (non-condensing).
Ambient temperature during the transportation and
storage of the instrument: between - 10° and + 50°C.
Relative humidity during the transportation and storage
of the instrument: max.80% (non-condensing).
The operator is responsible for checking that the instrument is working properly and safely.The operator shall assume
responsibility for any use of special equipment and/or materials for operating the instrument.
NOTE
If possible, retain the original packaging for the entire lifetime of the instrument so that you can repackage the
instrument correctly if it needs to be sent for servicing.
3.1 Transportation, unpacking and setting up the instrument
Operating instructions

14 pfm Rotary 3005 E Rev.2022-03-21
3.2 Assembling the accessories
Object orientation clamping lever
Object orientation adjustment screws
Orientable adapter
1
2
3
1
3
2
Adapter orientable
Assembling the object clamps with/without adapter
Universal cassette clamp,
orientable
Universal cassette clamp,non-
orientable (without adapter)
Release and remove the object orientation clamping lever.
Turn the two adjustment screws for object orientation to the tangible zero-point (marked in red).
Insert the object clamp with the adapter, paying attention to the position of the hole so that the clamping lever can

Clamp the object clamp using the clamping lever.

NOTE
Some object clamps are optionally also available as a non-orientable version. For use on the instrument, the object clamp

Operating instructions

15pfm Rotary 3005 E Rev.2022-03-21
Operating instructions
Knife holder
2
2
The knife holder consists of the knife holder base and the

to the disposable blade holder upper jaw.
Finger guard
Disposable blade holder upper jaw
Knife holder base
1
1
3
3
Assembling the knife holder base
2
Knife holder base
Knife holder base
Knife holder clamping levers
1
1
1
1
2
Guide rails
Knife holder mounting device
1
Release the knife holder clamping levers.

Clamp the knife holder mounting device using the knife holder clamping levers.

2
2
1

16 pfm Rotary 3005 E Rev.2022-03-21
Assembling the disposable blade holder upper jaw
2
Pressure plate
Clearance angle adjustment clamping lever
Pressure plate clamping lever
Knife angle scale
1
1
2
3
3
4
4
Release and completely remove the clearance angle adjustment clamping lever.
Insert the adapter on the disposable blade holder upper jaw into the knife holder base.Take note of the position of the

Set the clearance angle to 10° using the knife angle scale.
Clamp the clearance angle adjustment clamping lever

Control panel connection
Control panel connection
1Control panel
The colour-marked (green) connection for the control panel is located on the back of the instrument.
Connect the control panel cable to the control panel socket.
WARNING
Caution! Please observe the guide on the foot switch socket and do not turn the plug in the
connection socket.
1
Operating instructions

17pfm Rotary 3005 E Rev.2022-03-21
Section waste tray
The section waste tray is pushed onto the microtome via
two guides on the inside and then positioned so that it is
felt to latch.
Operating instructions
Fitting the microtome blade
2
Finger guard
Pressure plate
Blade support
Pressure plate clamping lever
1
1
2
3
3
4
4

Release the clamping lever for the pressure plate.
Remove the microtome blade from the dispenser and carefully slide it under the pressure plate from the side.
It is easier to introduce the microtome blade by gently pressing on the pressure plate.This forms a small gap between
the blade support and the pressure plate, making it easier to introduce and position the microtome blade.
Make sure that the microtome blade is positioned parallel to the pressure plate.
Clamp the pressure plate using the clamping lever.

Feather® microtome blade S35

18 pfm Rotary 3005 E Rev.2022-03-21
Fitting a standard knife

Loosen the two screws on the standard knife holder.
Insert the knife into the knife holder from the side.

the side.
Clamp the knife using the two screws.

2
Finger guard
Screws
Knurled screw
1
3
1
3
2
Operating instructions

must make sure that the supply voltage and frequency


Always connect the instrument to an earthed wall socket.
Plug the power cable into the socket on the back of the
instrument.
The insert for the instrument fuse (T2.0A) is situated next
to the connection socket.
Now switch on at the power switch on the instrument.
1=ON, 0=OFF
WARNING
Caution! Only use the mains power cable with
ferrite core supplied with the instrument.
Caution! Do not operate the instrument if the
mains power cable is damaged.
Caution! Do not use an extension cable.
Caution! If the instrument is not operated with
the mains power cable supplied, the user must
carry out a safety test with the new mains power
cable before using the instrument for the first
time.
3.3 Initial operation
Connection socket
Instrument fuse
Power switch
1
2
3
12 3

19pfm Rotary 3005 E Rev.2022-03-21
3.5 Operating controls
The pfm Rotary 3005 E is equipped with a modern touch
screen system.The control panels and displays are clear and
arranged by function.The user can operate the instrument
either via the operating display on the instrument or via a
separate control panel.This allows for simple and
comfortable operation.When the control panel is connected,
the instrument can only be controlled from the control
panel.The control panel is not included in the general scope
of delivery and must be ordered separately.
Operating instructions
After switching on the instrument an audible signal can be
heard, and “REFERENCING” is shown on the display The
reference run is started by pressing START . After this, the
display automatically goes back to the control panel display.
WARNING
Caution! Before starting the reference run always
move the knife holder to the front position.
Otherwise the knife holder may be damaged,
because, depending on the position of the object
holder, the latter moves downward during the
reference run.
Caution! Do not operate the instrument if the
display has no function, shows illogical or
incorrect values or is damaged.
3.4 Switching on the instrument
3.6 Operating display on the instrument/control panel
M-STOP
Section thickness
Trimming
Trimming automatically/manually
Retraction
Counter
Remaining feed
Menu
Memo
Delete
Control wheel Settings
Control wheel Coarse Feed/Trimming
1
2
3
4
5
6
7
8
9
10
11
12
1
2
3
4
5
6
7
98 10
11
12

20 pfm Rotary 3005 E Rev.2022-03-21
3.7 Control panel
If the pfm Rotary 3005 E is to be operated via the separate
control panel,it must be connected to the instrument
before switching it on.The connection for the control panel
is located on the back of the instrument.
Press the MENU button to get to a submenu with the
following subfunctions:
Buzzer
Press the BUZZER 
Language
Press the LANGUAGE 
languages.
Brightness
Press the BRIGHTNESS button to adjust the brightness of
the display.There are three levels in total.
Reset/factory default settings
Press the RESET button to reset the instrument to
factory defaults.
3.8 Menu settings
Operating instructions
By pressing the Trimming/Coarse Feed control wheel you
can switch between trimming and coarse feed.
3.9 Selecting Trimming and Coarse Feed modes