Pyxis SP-400 User manual

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SP-400 Handheld Fluorometer
User Manual
August 10, 2020
Rev. 1.31
Pyxis Lab, Inc.
1729 Majesc Dr. Suite 5
Lafayee, CO 80026 USA
www.pyxis-lab.com
© 2017 Pyxis Lab, Inc.
Pyxis Lab Proprietary and Condenal

Table of Contents
1 Introducon 3
1.1 MainFeatures......................................... 3
2 Specicaons 3
3 Unpacking Instrument 5
3.1 StandardAccessories ..................................... 5
3.2 OponalAccessories ..................................... 5
4 Installaon 5
4.1 BaeryInstallaon ...................................... 5
5 Instrument Overview 6
5.1 ControlKeys.......................................... 6
5.2 MainModuleOn/O ..................................... 7
6 Measurement 7
6.1 PTSA and Conducvity Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
6.2 Conducvity Temperature Compensaon . . . . . . . . . . . . . . . . . . . . . . . . . . 8
6.3 High Color and Turbidity Warning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
7 Calibraon 9
7.1 PTSA Calibraon (Two-Point with Zero) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
7.2 Conducvity Calibraon (500, 1000, 2500, or 5000 µS/cm) . . . . . . . . . . . . . . . . . 11
7.3 User-Dened Conducvity Calibraon . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
7.4 Combined PTSA and Conducvity Calibraon . . . . . . . . . . . . . . . . . . . . . . . . 14
8 Device Informaon and Diagnosis 15
8.1 Bluetooth Connecon to Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
8.2 FactoryReset ......................................... 18
9 Use with uPyxis® Mobile App 19
9.1 Download uPyxis® MobileApp................................ 19
9.2 Connecng to uPyxis® MobileApp.............................. 19
9.3 SengScreen......................................... 20
9.4 SystemScreen......................................... 21
10 Use with uPyxis® Desktop App 22
10.1 Install uPyxis® DesktopApp.................................. 22
10.2 Connecng to uPyxis® DesktopApp ............................. 22
10.3SystemScreen......................................... 23
10.4DatalogScreen ........................................ 24
10.5SengScreen......................................... 24

11 Device Maintenance and Precauon 25
11.1 Maintenance Best Pracces and Quick Tips . . . . . . . . . . . . . . . . . . . . . . . . . 25
11.2 Methods to Cleaning the SP-400 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
11.3Storage ............................................ 26
12 Contact Us 26
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Warranty Informaon
Condenality
The informaon contained in this manual may be condenal and proprietary and is the property of Pyxis
Lab, Inc. Informaon disclosed herein shall not be used to manufacture, construct, or otherwise reproduce
the goods described. Informaon disclosed herein shall not be disclosed to others or made public in any
manner without the express wrien consent of Pyxis Lab, Inc.
Standard Limited Warranty
Pyxis Lab warrants its products for defects in materials and workmanship. Pyxis Lab will, at its opon, repair
or replace instrument components that prove to be defecve with new or remanufactured components
(i.e., equivalent to new). The warranty set forth is exclusive and no other warranty, whether wrien or oral,
is expressed or implied.
Warranty Term
The Pyxis warranty term is thirteen (13) months ex-works. In no event shall the standard limited warranty
coverage extend beyond thirteen (13) months from original shipment date.
Warranty Service
Damaged or dysfunconal instruments may be returned to Pyxis for repair or replacement. In some in-
stances, replacement instruments may be available for short duraon loan or lease.
Pyxis warrants that any labor services provided shall conform to the reasonable standards of technical com-
petency and performance eecve at the me of delivery. All service intervenons are to be reviewed and
authorized as correct and complete at the compleon of the service by a customer representave, or des-
ignate. Pyxis warrants these services for 30 days aer the authorizaon and will correct any qualifying
deciency in labor provided that the labor service deciency is exactly related to the originang event. No
other remedy, other than the provision of labor services, may be applicable.
Repair components (parts and materials), but not consumables, provided during a repair, or purchased
individually, are warranted for 90 days ex-works for materials and workmanship. In no event will the in-
corporaon of a warranted repair component into an instrument extend the whole instrument’s warranty
beyond its original term.
Warranty Shipping
A Repair Authorizaon (RA) Number must be obtained from Pyxis Technical Support before any product can
be returned to the factory. Pyxis will pay freight charges to ship replacement or repaired products to the
customer. The customer shall pay freight charges for returning products to Pyxis. Any product returned to
the factory without an RA number will be returned to the customer. To receive an RMA you can generate a
request on our website at hps://pyxis-lab.com/request-tech-support/.
Pyxis Technical Support
Contact Pyxis Technical Support at [phone], [email protected], or by lling out a request for
support at hps://pyxis-lab.com/request-tech-support/.
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1 Introducon
The Pyxis SP-400 is a uniquely designed handheld mulmeter that measures both PTSA and conducvity,
simultaneously. It is a cuvee-less device. Less than 5 mL water sample is needed to ll the sample cell for
proper measurement.
1.1 Main Features
The SP-400 includes the following features:
• Simultaneous measurement of the electrical conducvity value and the concentraon of uorescent
tracer PTSA of a water sample.
• Pre-calibrated for measuring PTSA (pyrenetetrasulfonic acid) in the range of 0 to 300 ppb.
• The uorescence PTSA measurement is automacally compensated for sample color and turbidity
interference up to 60 NTU without preltraon.
• Conducvity and PTSA uorescence can be calibrated using a single combined standard through a
user-friendly, menu-driven procedure.
• Large color graphic screen that can be read in direct sunlight.
• A single conducvity calibraon using a single standard covers the whole measurement range of
0–15000 µS/cm.
2 Specicaons
Table 1. PTSA Specicaons
Item Specicaon*
Range 0–300 ppb
Accuracy ±1% or ±1.0 ppb
Resoluon 0.1 ppb
Excitaon Wavelength 365 nm
Emission Wavelength 410 nm
Wavelength Accuracy ±1 nm
Calibraon Soluon Point 0, 100, 200, and 300 ppb
*With Pyxis’s connuous improvement policy, these specica-
ons are subject to change without noce.
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Table 2. Conducvity Specicaons
Item Specicaon*
Range 0–15000 µS/cm
Accuracy (Range: 0–20
µS/cm)
±10% or ±0.5 µS/cm
Accuracy (Range:
20–10000 µS/cm)
±1%
Accuracy (Range:
10000–15000 µS/cm)
±2%
Resoluon 1 µS/cm
Time for Stable
Measurement
5 seconds
Calibraon Soluons 500 µS/cm, 1000 µS/cm, 2500 µS/cm, 5000 µS/cm, combined 1000 µS/cm
and 100 ppb PTSA, and user-dened (500–5000 µS/cm)
*With Pyxis’s connuous improvement policy, these specicaons are subject to change without noce.
Table 3. Temperature Specicaons
Item Specicaon*
Range 42–112 °F (5–45 °C)
Accuracy ±1%
Resoluon 0.2 °F (0.1 °C)
Compensaon Method Automac to 25 °C
*With Pyxis’s connuous improvement policy, these
specicaons are subject to change without noce.
Table 4. Other Specicaons
Item Specicaon*
Part Number (P/N) 50211
Display 320 ×240 TFT-LCD, visible under direct sunlight
Power Supply 4 AA alkaline baeries
Typical Baery Life 10,000 measurements
Dimension (L ×W×H) 6.30 ×2.91 ×1.30 inch (160 ×74 ×33 mm)
Weight 0.68 lbs (310 g)†
Operaonal Temperature 40–106 °F (4–41 °C)
Storage Temperature -4–140 °F (-10–50 °C)
Humidity 85% at 106 °F (41 °C)
Enclosure Rang IP67
*With Pyxis’s connuous improvement policy, these specicaons are subject to change
without noce.
†Baeries excluded
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3 Unpacking Instrument
Remove the instrument and accessories from the shipping container and inspect each item for any damage
that may have occurred during shipment. Verify that all items listed on the packing slip are included. If any
items are missing or damaged, please contact Pyxis Customer Service at [email protected].
3.1 Standard Accessories
• Four (4) AA alkaline baeries
• Bluetooth/USB Adapter for Desktop P/N: MA-NEB
• User Manual available online at www.pyxis-lab.com/support.html
3.2 Oponal Accessories
The following oponal accessories can be ordered from Pyxis Customer Service ([email protected]om) or
Pyxis eStore at hps://pyxis-lab.com/shop/.
Table 5. Oponal Accessories
Accessory Name Part Number (P/N)
Pyxis Carrying Case for Handheld Devices 50725
Pyxis 100 ppb PTSA + 1000 µS/cm (KCl) Combined Standard — 500 mL 21004
Pyxis Handheld Cleaning Kit SER-002
4 Installaon
4.1 Baery Installaon
The SP-400 is powered by four alkaline baeries. Typical baery life lasts for 10,000 measurements or 10
months. When the baery capacity is crically low, the SP-400 will display a ”LOW BATTERY” warning for 5
seconds and then automacally turn o.
*NOTE*Do not use rechargeable nickel cadmium (NiCad) or lithium baeries.
Replace the baeries to resume operaon of the SP-400 aer the baery warning. The SP-400 will auto-
macally turn on in the measurement mode aer new baeries are installaon.
The SP-400 baery compartment, shown in Figure 1, is on the back side of the instrument. Baeries are
held in place by a cover secured with two Phillips-head screws.
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Figure 1. The SP-400 baery compartment
Install baeries using the following steps:
1. Remove the baery compartment cover by loosening the two screws.
2. Remove old baeries and dispose of them properly.
3. Following the posive and negave terminal signs in the compartment boom, snap four new AA
alkaline baeries rmly into the baery holder.
4. Replace the baery compartment cover and ensure that the sealing O-ring is lying at on the baery
holder.
5. Fasten the two screws.
*NOTE*Failure to properly seat the O-ring may result in water damage to the SP-400.
5 Instrument Overview
5.1 Control Keys
The SP-400 has three control keys, as shown in Figure 2. The le ( ), right ( ), and ok ( ) keys
are used to launch acons indicated on the LCD display directly above the keys. The labels above the keys
indicate the funcon associated with each key and funcons can be changed in dierent operaon modes.
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Figure 2.
5.2 Main Module On/O
To turn on the SP-400: Press momentarily and release.
To turn o the SP-400: Press and hold for about three seconds. Release when the LCD display
turns o. The SP-400 turns itself o aer 60 seconds without user interacon detected. This is done to
conserve baery life.
*NOTE*This auto-me o seng may be customized by the user as desired through the
uPyxis® Mobile or Desktop App.
6 Measurement
6.1 PTSA and Conducvity Measurement
When powered on, the SP-400 will default to the PTSA and conducvity measurement mode. The water
sample can be transferred to the sample cell using a pipee or lled directly from a faucet, sample bole,
or sample valve.
*NOTE*Special care should be taken when pouring the sample into the cell to avoid air
bubble entrainment, which can interfere with reading accuracy.
Before beginning a measurement, use the sample water to rinse the sample cell at least three mes. Allow
5–10 seconds for the SP-400 to stabilize. The values will be displayed in white with a blue background
if a stable value is reached (Figure 2). For a sample with conducvity in the range of 100 to 6000 µS/cm,
the measured value should be stabilized in the range of 98–102 to 5940–6060 µS/cm, respecvely. For a
sample containing 100 ppb PTSA, the measured PTSA should be stabilized within the range of 98–102 ppb.
*NOTE*The me required to reach a stable reading may be slightly longer if the water
sample temperature is signicantly dierent than the environmental temperature at which
the SP-400 had been equilibrated (stored).
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