Pyxis SP-800 User manual

SP-800 Portable Water Analyzer
Operation Manual
Rev.B
©Pyxis Lab, Inc 2017
All Rights Reserved
Pyxis Lab, Inc
1729 Majestic Dr Suite 5
Lafayette, CO 80026 USA
www.pyxis-lab.com
[e-mail]

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Contents
1 General Description ..............................................................................................................................4
1.1 Specification..................................................................................................................................4
1.2 Pyxis Major Features.....................................................................................................................5
1.3 Unpackaging the Instrument ........................................................................................................5
1.4 Standard Accessories ....................................................................................................................5
1.5 Optional Accessories.....................................................................................................................6
1.6 Sample Vial Compartment............................................................................................................6
1.7 Light Shield Cover..........................................................................................................................6
2 Start the SP-800 ....................................................................................................................................8
Battery Installation........................................................................................................................8
2.1 Description of the Navigational Control Pad ................................................................................8
2.2 Turning on the SP-800...................................................................................................................8
2.3 Main Page .....................................................................................................................................9
2.4 Turning off the SP-800 ..................................................................................................................9
2.5 The SP-800 Auto Power off...........................................................................................................9
2.6 Auto LCD Power Saving.................................................................................................................9
3 Colorimetric Measurement.................................................................................................................10
3.1 Supported Methods....................................................................................................................10
3.2 Select a Method..........................................................................................................................12
3.3 Single Timing Step Method.........................................................................................................12
3.4 Single-Vial Procedure..................................................................................................................13
3.5 Two-Vial Methods.......................................................................................................................13
3.6 Multiple Timing Steps Method ...................................................................................................14
3.7 Advanced Methods.....................................................................................................................14
3.7.1 Low range, direct reading chlorine dioxide, 0 to 35.0 ppm................................................15
3.7.2 Turbidimetric Anionic Polymer Method .............................................................................15
3.7.3 Direct Reading Bleach Percent Method, 0 to 15%..............................................................15
3.8 Method Setup and Calibration....................................................................................................15
3.8.1 Set up the method parameters...........................................................................................15
3.8.2 Slope Calibration.................................................................................................................16

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3.8.3 Reagent Blank Calibration...................................................................................................17
3.8.4 Resume to Default Calibration Parameters ........................................................................17
4 Turbidity Measurement......................................................................................................................17
4.1 Operation....................................................................................................................................17
4.2 Turbidity Calibration ...................................................................................................................18
5 Absorbance Measurement .................................................................................................................19
6 16 mm Tube Adapter ..........................................................................................................................20
7 Bluetooth Interface.............................................................................................................................20
7.1 Install Software ...........................................................................................................................21
7.2 Turn on SP-800 Bluetooth...........................................................................................................21
7.3 Connect uPyxis to SP-800............................................................................................................21
7.3.1 Upgrade Firmware ..............................................................................................................21
7.3.2 Add User Defined Colorimetric Methods............................................................................22
8.3.4. Download Datalog.....................................................................................................................23
8 Maintenance.......................................................................................................................................24
9 Troubleshooting..................................................................................................................................25
10 Appendix A......................................................................................................................................26

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Trademarks and Patents
Hach ® is a registered trademark of the Hach Company, Loveland, CO USA
Confidentiality
The information contained in this manual may be confidential and proprietary and is the property of Pyxis Lab.
Information disclosed herein shall not be used to manufacture, construct, or otherwise reproduce the goods disclosed
herein. The information disclosed herein shall not be disclosed to others or made public in any manner without the
express written consent of Pyxis Lab.
Standard Limited Warranty
Pyxis Lab warrants its products for defects in materials and workmanship. Pyxis Lab will, at its option, repair or replace
instrument components that prove to be defective with new or remanufactured components (i.e., equivalent to new).
The warranty set forth is exclusive and no other warranty, whether written or oral, is expressed or implied.
Warranty Term
The Pyxis warranty term is thirteen (13) months ex-works. In no event shall the standard limited warranty coverage
extend beyond thirteen (13) months from original shipment date.
Warranty Service
Damaged or dysfunctional instruments may be returned to Pyxis for repair or replacement. In some instances,
replacement instruments may be available for short duration loan or lease.
Pyxis warrants that any labor services provided shall conform to the reasonable standards of technical competency
and performance effective at the time of delivery. All service interventions are to be reviewed and authorized as correct
and complete at the completion of the service by a customer representative or designate. Pyxis warrants these services
for 30 days after the authorization and will correct any qualifying deficiency in labor provided that the labor service
deficiency is exactly related to the originating event. No other remedy, other than the provision of labor services, may
be applicable.
Repair components (parts and materials), but not consumables, provided in the course of a repair, or purchased
individually, are warranted for 90 days ex-works for materials and workmanship. In no event will the incorporation of a
warranted repair component into an instrument extend the whole instrument’s warranty beyond its original term.
Shipping
A Repair Authorization Number (RA) must be obtained from the Technical Support ([email protected]) before any
product can be returned to the factory. Pyxis will pay freight charges to ship replacement or repaired products to the
customer. The customer shall pay freight charges for returning products to Pyxis. Any product returned to the factory
without an RA number will be returned to the customer.

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1General Description
1.1 Specification
•Colorimeter Wavelength: 365/420/455/525/560/570/630 nm
•Turbidity Excitation Wavelength: White/infrared LED/90-degree scattering
•Absorbance Reproducibility: 0.005 au (0 - 1.0 au) (3sigma)
•Absorbance Linearity Range: 0 to 1.0 au
•Turbidity Reproducibility: 1 NTU (3 sigma)
•Turbidity Detection Limit: 1 NTU
•Turbidity Range: 0 - 200 NTU
•Battery: 4 AA alkaline
•Typical Battery Life: 3 months
•Display: Graphical LCD 160x240 pixels, visible under direct sunlight
•Instrument Dimension: L 265mm W 88mm H 62mm
•Instrument Weight: 600 g without batteries
•Storage Temperature Range: 0 to 140°F (-18 - 60°C)
•Operation Temperature Range: 40 to 120 °F (4 - 49°C)
•Humidity: 85% at 106 °F (41 °C)
•Environmental: IP67, dustproof and waterproof
Note:
1. Specifications are subject to change without notice with Pyxis’ continuous development.

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1.2 Pyxis Major Features
The SP-800 is a multi-wavelength colorimeter. It provides colorimetric measurements at 7 LED
wavelengths and nephelometric turbidity measurement using white LED and infrared LED as the
excitation sources. The SP-800 is pre-calibrated for colorimetric measurements of analyses common
in industrial water treatment and other water testing in the laboratory or in the field, such as chlorine,
phosphate, iron, and copper. Main features include:
⚫The SP-800 is pre-calibrated for measuring turbidity in the range of 0 to 200 NTU.
⚫Automatically select the primary wavelength according to the method selected and switches to the
secondary wavelength to extend the primary measurement range.
Figure 1. Sample Vial and Major Components
⚫Display a concentration-time profile curve during the last time period in a colorimetric measurement.
The user can terminate the timing process and take a reading if the displayed concentration
reaches a plateau before completing the predefined time period.
⚫The user can update the calibration parameter of any pre-calibrated colorimetric method by testing
a standard solution first and then following a setup procedure to update the calibration parameters.
⚫Built-in Bluetooth allows easy connection to PC or mobile apps for downloading datalog and adding
new colorimetric methods.
⚫Pyxis specific methods such as non-cyanide containing zinc reagent are available.
⚫More wavelengths including near UV 365 nm are available than other colorimeters.
1.3 Unpackaging the Instrument
Remove the instrument and accessories from the shipping container and inspect each item for anydamage
that may have occurred during shipping. Verify that all items listed on the packing slip are included. If any
items are missing or damaged, please contact Pyxis Customer Service at servic[email protected].
1.4 Standard Accessories
•Sample Vials - two 10 ml (Part # MA-24), round, 0.78 inch (20 mm) pathlength, glass vials, which can
be used for all measurements including turbidity.
•4 AA alkaline batteries
•16 mm tube adapter (Part # 52214)
•Instrument Manual, also available from www.pyxis-lab.com
Navigational Control Pad
LCD
Sample Vial Compartment
Light Shield Cover
Sample Vial

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1.5 Optional Accessories
•25 ml sample vial (Part # MA-25)
•10 ml sample vial (Part # MA-24)
1.6 Sample Vial Compartment
The sample vial compartment is shown in Figure 1, along with a 10-ml sample vial. When the sample vial
is inserted into the sample vial compartment, the triangular mark on the sample vial should be aligned
approximately with the 6 o’clock position of the sample vial compartment or any position consistently.
The 16 mm tube adapter is needed for colorimetric methods using the 16 mm sample tube. The instruction
to us the adapter is provided in section 6.
The sample vial compartment can take in a 25 ml sample vial. The light shield cover is not required to be
closed if the 25 ml sample via is used.
The sample vial compartment should be kept clean. A small amount foreign material could significantly
affect turbidity measurement results. Use a soft cloth or lint free paper tissue to clean sample vial
compartment periodically. Remove debris, scale, and deposit promptly.
1.7 Light Shield Cover
The light shield cover is shown in Figure 2. The light shield cover can be conveniently slid between the
open and closed positions. The light shield cover is held firmly at the rest positions by permanent magnets.
The light shield cover should be in the closed position during storage, transportation, and measurements,
especially during the turbidity. When turned on, the SP-800 carries out self-diagnosis including checking
the performance of a variety of optical devices. The light shield door shall be at the closed position to shield
interference from ambient light during self-diagnosis.
Care should be taken to avoid water or debris being trapped in the track of the light shield door.

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Figure 2 Open and Close the Light Shield Cover
Warning
Magnetic sensitive devices, including but not limited to, credit cards, watches, hard disks, should be
keep at a distance of at least 2 inches from the Light Shield Door to avoid possible damage and/or loss
of information recorded.

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2Start the SP-800
Battery Installation
The SP-800 is powered by four AA-size alkaline batteries. Do not use rechargeable nickel cadmium (NiCad)
batteries or any AA-size lithium batteries. A set of batteries typically lasts for three months. When the
batteries capacity is low, the SP-800 will prompt a LOW BATTERY warning. Replace all four batteries to
resume operation of the SP-800 after the battery warning.
The SP-800 battery compartment, shown in Figure 3, is on the back side of the instrument. Insert a small
pad underneath the screen area to make the back-surface level when the instrument is turned upside down.
Install batteries as followings:
1. Remove the battery compartment cover by loosening four screws.
2. Insert four batteries into the battery holder as shown in Figure 3. Make sure the positive battery
polarity marker (+) is aligned with the positive marker (+) on the battery holder.
3. Replace the battery compartment cover, making sure that the sealing O-ring is lying flat on the
battery holder and tighten the four screws.
Figure 3 Replace Batteries
2.1 Description of the Navigational Control Pad
The SP-800 navigational control pad consists of five keys as shown in Figure 1. The left, right, up, and
down keys are navigational keys that are used to select an icon, a button, or other items in various pages.
The center key is the OK key. Press the OK key on a selected item to launch the action associated with the
selected item. The OK key is also used to accept the current selection, like the return key in a computer
keyboard.
2.2 Turning on the SP-800
After new batteries installation, the SP-800 will not be automatically turned on. To turn
on SP-800, press the OK key, and release the OK key when the LCD is lit.

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You can navigate the main page menu and launch an operation by pressing on an icon. If battery voltage
is too low for the instrument to work properly, the SP-800 will show a low battery warning message when it
is being turned on If this happens, replace all four batteries.
2.3 Main Page
The SP-800 provides intuitive icon driven user operations. On the main page, four major feature groups are
illustrated as below:
Figure 4. Main Menu
A brief description of each feature group is given in Table 1. Detailed operation instructions can be found
in the following chapters.
Table 1 Feature Groups on Main Menu
No.
Title
Description
3
COLOR
Colorimetric measurement methods
5
Power
Turn off SP-800
6
ABSB
Absorbance measurements
8
SYS
System information
2.4 Turning off the SP-800
Turn the SP-800 off by navigating to Power icon and press the OK key. Alternatively,
you can turn off the SP-800 by pressing OK key for 5 seconds in any menu.
2.5 The SP-800 Auto Power off
The SP-800 automatically turns itself off with no-key activity for a given period, except
for during a measurement. The auto power-off time can be set in SYS->System Set.
Pressing OK key will wake up the instrument, and the SP-800 will return to the original
page if it has any measurement data.
2.6 Auto LCD Power Saving
During a colorimetric method measurement, The SP-800 automatically turns LCD backlight off with no-key
activity and continues the measurement with the LCD backlight off. The auto LCD power-off time can be
set in SYS->System Set. Pressing any key will turn on the LCD backlight. Under normal ambient lighting
condition, icons and other contents shown on the LCD screen are readable without backlight being on.
2016/07/19 08:00
Power
M-F TURB
ABSB SYS
M-P
CAL
COLOR

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3Colorimetric Measurement
3.1 Supported Methods
A wide range of colorimetric methods is supported by the SP-800 analyzer and the number of them keeps
increasing with continuous development of Pyxis. See corresponding Hach® methods in Appendix A.
Table 2 List of Supported Colorimetric Methods
Abbreviated Method
Name
Method Name
Description
Range
CL-F
F-Chlorine
Free chlorine, DPD method
2.2 ppm
CL-T
T-Chlorine
Total chlorine, DPD method
2.2 ppm
CL2H
CL2High
High range, DPD method
6.0ppm
CuBi
Cu_Bicinch
Bicinchoninate, EPA approved
for reporting wastewater
analysis
5.0 ppm
DEHA
DEHA
Method for N,N-
diethylhydroxylamine and other
oxygen scavengers
0.5 ppm
Ca
Ca
Calmagite method for calcium
4.00 ppm as
CaCO3
Mg
Mg
Calmagite method for
magnesium
4.00 ppm as
CaCO3
FePh
Fe_phenanth
Total iron using 1,10-
phenanthroline, USEPA
approved for reporting
wasterwater analysis
3.00 ppm
FeZi
FeZine
FerroZine method
1.300 ppm
FeTp
FeTptz
Total iron using TPTZ
1.80 ppm
MoHR
Mo_HighRange
High range molybdate
40.0 ppm
MoLR
Mo_LowRange
Low range molybdate using
ternary complex method
3.0 ppm
NO2H
NO2H
High range nitrite, ferrous
sulfate method
150 ppm as
NO2
NO2L
NO2L
Low range nitrite, diazotization
method, USEPA approved for
reporting wastewater and
drinking water analysis
0.350 ppm
as NO2
PMoV
OPO4-MoV
Reactive phosphate using
molybdovanadate method
30.0 ppm as
PO4
OPO4
OPO4
Reactive phosphate using
ascorbic acid molybdenum blue
method, USEPA accepted for
wastewater analysis
2.5 ppm as
PO4
OrgP
Phosphonate
UV digestion and ascorbic acid
reduction molybdenum blue
method
7.1 ppm as
PBTC
PAmi
OPO4-Amino
Reactive phosphate, amino
acid reduction method
30.0 ppm
ClO2
ClO2-DPD
DPD method, USEPA accepted
for reporting drinking water
analysis
5.00 ppm
ClO2D
ClO2Direct
Direct method for chlorine
dioxide
35.0 ppm

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Abbreviated Method
Name
Method Name
Description
Range
SiHR
SiHR
High range silica
75.0 ppm as
SiO2
SiLR
SiLR
Low range silica
1.60 ppm as
SiO2
AZOL
Azole
UV digestion for tolyltriazole
and benzotriazole
16.0 ppm
SO4
SO4
Barium sulfate turbidimetric
method
70.0 ppm
POLY
Polymer
Turbidimetric method for
anionic polymeric dispersant
13.0 ppm as
PAA
FeMo
FeMo
Total iron method for water
containing molybdate
1.80 ppm
Cr6
Cr6
1,5-Diphenylcarbohydrazide
method for chromium
hexavalent, USEPA accepted
for wastewater analyses
0.6ppm
CrT
CrTot
Alkaline hypobromite Oxidation
method for chromium total
0.6ppm
NH3S
NH3Sal
Salicylate method for nitrogen,
ammonia
0.5ppm
NH2C
NH2CL
Indophenol method for
chloramine mono
3.0ppm
N2H4
N2H4
p-Dimethylaminobenzaldehyde
method for hydrazine
0.5ppm
MnL
MnLow
Low range manganese
0.7ppm
MnH
MnHigh
High range manganese
20.0ppm
BLCH
Bleach
Direct Method measuring
sodium hypochlorite
concentration
AL
Alumi
Aluminon method for aluminum
0.8ppm
F
Floride
SPADNS method for fluoride
2.0ppm
CuL
CuPorp
Porphyrin Method for copper
0.2ppm
Zn
Zinc
Zincon method for zinc, USEPA
approved for wastewater
analysis
3.0ppm
S2-
Sulfide
Methylene blue method for
sulfide, USEPA accepted for
reporting wastewater analysis
0.7ppm
CN
Cyanide
Pyridine-Pyrazalone method for
cyanide
0.2ppm
NO3M
NO3M
Middle range nitrate
5ppm as N
NO3H
NO3H
High range nitrate
30ppm as N
Ni
Ni
PAN method for nickel
1ppm
CYAN
CYAN
Turbidimetric method for
cyanuric acid
55ppm
pH
pH
Phenol red method for pH
8.5

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3.2 Select a Method
Move the icon focus to the method icon COLOR using the navigational (left, right, up, or down) keys. Press
OK on the icon to launch the first method selection page. The methods shown on the top row of the page
are the most frequently selected methods.
Figure 5. Method Selection
The followings are the operations associated with this page:
1. Use the navigational keys and the OK key to select and launch a method.
2. Long press the OK key to return to the main page. Press the arrow icon at the lower right corner of
the page to display the second method selection page if the device is loaded with more than 23
methods.
Note: Methods shown in the method selection pages include Hach© equivalent methods and Pyxis specific
advanced methods. The table in Appendix A provides a brief description of Pyxis method names and their
corresponding Hach® program number. Hach® reagents for 10 ml sample can be used for the test.
3.3 Single Timing Step Method
Most of colorimetric methods have only one timing step. As an example, in the DPD free chlorine method,
it takes one minute for the DPD powder reagent to completely react with chlorine in the water sample. The
DPD free chlorine method has a single one-minute timing step. Figure 7 shows the main page of a method
with a single timing step.
Figure 6. Single Timer Method

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3.4 Single-Vial Procedure
1. Place the sample vial filled with the water sample in the SP-800 sample vial compartment and press
the ZERO button. The SP-800will display the page shown in Figure 7.
2. Take the sample vial out and add the reagent to the sample vial.
3. Place sample vial back into the sample vial compartment and press the timer button TMR1. The
SP-800 will start to monitor the reaction between the reagent and the species you want to measure
in the water sample. The concentration is shown in the chart as a function of time (Figure 8).
4. When the timer reaches the preset time and the reaction is complete, the value of concentration
will be shown on the top right corner of the page.
5. The rate of the reaction is often faster than the standard pre-set time, which will become apparent
from the concentration-time plot. You can press the STOP button to stop the timer and terminate
the timing step. The last read concentration value will be displayed on the top right corner of the
page after you terminate the timing step.
Figure 7. Concentration as a Function of Time
3.5 Two-Vial Methods
Some colorimetric methods require using two vials. The water sample is added to two identical vials. One
vial is being used to zero the colorimeter, referred as to the prepared blank. A reagent is added to the other
vial, referred as to the prepared sample. The absorbance value is determined from the prepared sample.
If the method requires two or more reagents, the prepared blank could be the resulting solution after one
or more reagents have been added to the sample.
The following procedure is typical for two-vial methods:
1. Place the prepared blank into the SP-800 sample vial compartment and press the ZERO button to
zero the instrument.
2. Place the prepared sample into the SP-800 sample vial compartment and press the TMR1 button
to start the method timer.
3. When the timing step is completed, the measured concentration will be displayed on the top of the
page. The timing step could be terminated earlier by pressing STOP button.
4. Optionally, the SP-800 can be re-zeroed using the prepared blank after the timing step is completed
or terminated. The blank reading will be subtracted from the measured concentration value, and
the displayed concentration value on the top-right corner will be updated. This step is optional. It is
only necessary if the prepared blank changes its color during the timing period.
5. Optionally, the prepared sample vial can be put back and read again by pressing the READ button
if the blank is re-zeroed after the timing step is completed or terminated. A new concentration value
based on the last absorbance value measured will be calculated and displayed.

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3.6 Multiple Timing Steps Method
Some colorimetric methods have two or three timing steps. The SP-800 shows a count-down timer for the
timing steps before the last timing step (Figure 9). During these timing steps, one or more reagents are
added to the sample, or operations such as swirling the vial to mix the reagent and the sample are being
performed. These methods usually use one vial for the prepared blank and the other for the prepared
sample.
Figure 8. Multiple Timer Method
In order to show the concentration-time curve as shown in Figure 8 during the last timing step, The SP-800
must be zeroed using the prepared blank before the last timing step. Thus, the last timer button will not be
selectable until the SP-800 has been zeroed using the prepared blank. Multi-timing step Hach® methods
require zeroing the colorimeter using the prepared blank after the last timing step is completed. The SP-
800 can optionally be re-zeroed using the prepared blank after the last timing step. The blank value
measured will be subtracted from the concentration value measured at the end of the last timing step.
Optionally, the READ button could be pressed to read the prepare sample again.
The following procedure is typical for methods having two-timing steps:
1. Press the TMR1 button to start the first timer. Complete the necessary operations to prepare
the blank and the sample.
2. Place the prepared blank into the SP-800 sample vial compartment and press the ZERO
button.
3. Place the prepared sample into the SP-800 sample vial compartment and press the TMR2
button to start the second timer. The SP-800 will display the measured concentration as a
function of time as shown in Figure 8.
4. When the timing step is completed, the measured concentration will be displayed on the top
right of the screen. The timing step could be terminated earlier by pressing STOP button.
5. Optionally, The SP-800can be re-zeroed using the prepared blank after the timing step is
completed or terminated. The blank reading will be subtracted from the measured
concentration value, and the displayed concentration value on the top-right corner will be
updated. This step is optional. It is only necessary if the prepared blank changes its color
during the timing period.
3.7 Advanced Methods
The SP-800 provides 7 LED wavelengths and can measure absorbance values at multiple LED
wavelengths. Consequently, the SP-800 can provide many predefined advanced methods that traditionally
require complex and often expensive lab testing procedures.

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Low range, direct reading chlorine dioxide, 0 to 35.0 ppm
The maximum absorption bank of aqueous chlorine dioxide is around 360nm. The SP-800 has a 365nm
UV LED and can be used to directly measure chlorine dioxide. It offers a much lower detection limit (0.2
ppm) than direct methods available from other portable colorimeters having only light sources in the visible
range.
Select ClO2D in the method selection page and carry out the following steps to measure chlorine dioxide:
1. Place a vial filled with deionized water into the vial compartment and press the ZERO button
to zero the SP-800.
2. Discard the deionized water and fill the same vial with the sample. Place the vial into the vial
compartment and press READ button to read. The measured chlorine dioxide concentration
will be displayed in the top of the method page.
Turbidimetric Anionic Polymer Method
1. Add polymer reagent 1 to 10 ml sample and inverse the sample vial 5 times to mix the reagent
with the sample. Place the sample via to the sample vial compartment.
2. Press on ZERO.
3. Add polymer reagent 2 and press on TMR1 to start the five minutes timer.
4. Gently inverse the sample via for 10 times and place the sample vial to the sample vial
compartment.
5. Polymer concentration will be measured and displayed when the five-minute timer is reached.
The polymer concentration is shown as ppm PAA (polyacrylic acid) equivalent.
Direct Reading Bleach Percent Method, 0 to 15%
The SP-800 has a 365nm UV LED and other deep blue LEDs that can be used to directly measure bleach
concentration in the range of 0 to 15%. No reagent is required for the method and the displayed result is
the sodium hypochlorite concentration in percentage.
Select BLCH in the method selection page and carry out the following steps:
1. Place a vial filled with deionized water into the vial compartment and press the ZERO button
to zero the SP-800.
2. Discard the deionized water and fill the same vial with the bleach sample. Place the vial into
the vial compartment and press READ button to read. The measured bleach concentration will
be displayed in the top of the method page.
3.8 Method Setup and Calibration
Press the SETUP button in the method result page to launch the method setup and calibration page.
Set up the method parameters
Press the FORM button to select a concentration form from the list of forms that are available for this specific
method (Figure 10).
Press the UNIT button to select a concentration unit among the list of ppb, ppm, mg/L, ug/L and No Unit
(Figure 11).

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Figure 9. Method from Selection
Figure 10. Method Unit Selection
Slope Calibration
If the method has been calibrated prior to shipping, there is no need to calibrate unless a calibration check
indicates that the method needs a calibration. The following steps are used to calibrate a method:
1. Use a calibration standard of known concentration. Follow the steps required by the method and note
the value reported by the SP-800.
2. If the measured value differs from the known standard value, Press the CONFG button to launch the
method configuration page.
3. Press the slope calibration button SlpCal. A numeric keyboard will be displayed.
4. Enter the concentration value and press the OK key on the enter key in the numeric keyboard to
return to the configuration page.
5. Press the EXIT button. Press the OK key to accept the calibration or other key to cancel the calibration.

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Figure 11. Slope Calibration
For best results, the concentration of the standard solution should be less than the maximum concentration
for the method (table 2) and greater than the half of the maximum concentration. For example, to calibrate
total chlorine, the chlorine concentration in the standard solution should be between 1.1 and 2.2 ppm.
The corresponding calibration parameters will be updated and saved in the memory as the working
calibration parameter set. Note that this set of calibration parameters are not the same as the default set.
You can use Default button to copy the default calibration parameters to the working set.
Reagent Blank Calibration
Some methods have a non-zero intercept value in the calibration equation. For these methods, a proper
non-zero intercept value is pre-loaded in the SP-800 prior to shipping. The following steps are used to carry
out a reagent blank calibration:
1. Follow the normal steps to carry out a measurement on a deionized water sample.
2. Press the CONFG button to launch the method configuration page.
3. Press the reagent blank calibration button BLKC
4. Press the OK key to save when exiting from the configuration page or press other keys to
cancel.
Resume to Default Calibration Parameters
Pressing the Default button will copy the default calibration intercept and slope to the working intercept and
slope, respectively. If the default calibration parameters were created prior to shipping, this button action is
to restore the working calibration parameters to the original factory loaded calibration parameters.
4Turbidity Measurement
4.1 Operation
Follow the following steps to measure turbidity:
1. Fill the 10 ml sample vial to above the 10 ml mark.
2. Insert the sample vial to the sample vial compartment.
3. Slide the light shield cover to the closed position.
4. Press the TURB on the main page, then press the OK key, The SP-800 will start to measure
the turbidity in the sample.

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4.2 Turbidity Calibration
1. Fill the 10 ml sample vial to above 10 ml mark with the deionized water.
2. Insert the sample vial to the sample vial compartment.
3. Slide the light shield cover to the closed position.
4. Press the CAL on the main page, then choose the Turbidity calibration and press the OK button
to launch the Turbidity calibration page. (Figure 13)
5. Press the OK key to measure the deionized water
6. Fill the 10 ml sample vial to above 10 ml mark with the 50 NTU standard. Insert the sample vial
to the sample vial compartment.
7. Press the OK key to measure the 50 NTU standard. Low range turbidity calibration is
successful
8. Press the OK key to continue high range turbidity calibration. If high range turbidity calibration
not required, press any keys to exit. (Figure 14)
9. Fill the 10 ml sample vial to above 10 ml mark with the 100 or 200 NTU standard. Insert the
sample vial to the sample vial compartment.
10. Follow the message prompts, use the upper or down key to switch the standard between 100
NTU and 200 NTU.
11. Press the OK key to measure the selected standard. High range turbidity calibration is
successful. (Figure 15)
12. Press any keys to exit.
Figure 12. Turbidity Calibration-1
Figure 13. Turbidity Calibration-2

19
Figure 14. High Range Turbidity Calibration
5Absorbance Measurement
The following steps are used to measure the absorbance values of a sample:
1. Press the ABS to launch the absorbance measurement page.
2. Place a vial filled with the blank sample in the sample vial compartment. Press the ZERO button
to zero the method.
3. Place a vial filled with the sample in the sample vial compartment. Press the READ button to
read absorbance. The absorbance values of first 6 wavelengths (Table 3) will be shown. Press
the READ button again to show the absorbance values of the last three wavelengths.
Figure 15. Absorbance Measurement
Press EXIT to return to the main page. Timing function for absorbance measurement may not be available
for some models.
Table 3 Wavelength of each channel
Channel
Wavelength (nm)
1
560
2
570
3
Not used
4
Not used
5
455
6
525