Pyxis SP-395 HST Quick start guide

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SP-395 HST
Fluorometer
Operational Manual
Version 1.3 November 2018

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Table of Contents
1. General Description 4
1.1.Specification 4
1.2.SP-395 Features 4
1.3.Unpacking the Instrument 4
1.4.Standard Accessories 5
1.5.Optional Accessories 5
1.6.Light Shield Cover 5
2. Starting SP-395 6
2.1.Battery Installation 6
2.2.Description of Control Keys 7
2.3. Turning On/Off SP-395 8
3. HST Measurement 8
3.1. Measurement 8
3.2. High Color and Turbidity Warning 9
4. Calibration 10
5. Device Information and Diagnosis 12
6. Wireless Connection 12
7. How to Clean SP-395 13

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Confidentiality
The information contained in this manual may be confidential and proprietary and is the property of
Pyxis Lab Inc. Information disclosed herein shall not be used to manufacture, construct, or otherwise
reproduce the goods disclosed herein. The information disclosed herein shall not be disclosed to others
or made public in any manner without the express written consent of Pyxis Lab Inc.
Standard Limited Warranty
Pyxis Lab warrants its products for defects in materials and workmanship. Pyxis Lab will, at its option,
repair or replace instrument components that prove to be defective with new or remanufactured
components (i.e., equivalent to new). The warranty set forth is exclusive and no other warranty,
whether written or oral, is expressed or implied.
Warranty Term
The Pyxis warranty term is thirteen (13) months ex-works. In no event shall the standard limited
warranty coverage extend beyond thirteen (13) months from original shipment date.
Warranty Service
Damaged or dysfunctional instruments may be returned to Pyxis for repair or replacement. In some
instances, replacement instruments may be available for short duration loan or lease.
Pyxis warrants that any labor services provided shall conform to the reasonable standards of technical
competency and performance effective at the time of delivery. All service interventions are to be
reviewed and authorized as correct and complete at the completion of the service by a customer
representative, or designate. Pyxis warrants these services for 30 days after the authorization and will
correct any qualifying deficiency in labor provided that the labor service deficiency is exactly related to
the originating event. No other remedy, other than the provision of labor services, may be applicable.
Repair components (parts and materials), but not consumables, provided in the course of a repair, or
purchased individually, are warranted for 90 days ex-works for materials and workmanship. In no event
will the incorporation of a warranted repair component into an instrument extend the whole
instrument’s warranty beyond its original term.
Shipping
A Repair Authorization Number (RA) must be obtained from the Technical Support (service@pyxis-
lab.com) before any product can be returned to the factory. Pyxis will pay freight charges to ship
replacement or repaired products to the customer. The customer shall pay freight charges for returning
products to Pyxis. Any product returned to the factory without an RA number will be returned to the
customer.

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1. General Description
1.1. Specification
Measurement Range
0.1-7.5 ppm HST
Detection Limit
0.1 ppm HST
Accuracy
±0.1 ppm HST
Battery
9V alkaline battery
Typical Battery Life
3200 readings (480mAh battery)
Display
320x240 TFT-LCD, visible under direct sunlight
Dimension
L160 W74 H33 (mm)
Weight
310g (without battery)
Temperature Range
40 to 106 ℉(4 to 41 ℃)
Humidity
85% at 106 ℉(41 ℃)
Environmental
IP67, dustproof and waterproof
1.2. SP-395 Features
The Pyxis SP-395 analyzer is an ultraviolet fluorometer. It measures Halogenated tolytriazoles (HST) in a
water sample. Main features include:
•Sample acidification is not required. HST measurement is independent of sample pH in the
range of 6 to 10.
•Extra color and turbidity parameters measured are used for automatic compensation to
eliminate interference.
•No sample cuvette is needed and variations associated with the cuvette is eliminated.
•Large color graphic screen that can be read in direct sunlight.
•A single conductivity calibration using a single standard covers the whole measurement range of
0 to 15000 µS/cm.
1.3. Unpacking the Instruments
Remove the instrument and accessories from the shipping container and inspect each item for any
damage that may have occurred during shipping. Verify that all items listed on the packing slip are
included. If any items are missing or damaged, please contact Pyxis Customer Service at
[e-mail].

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1.4. Standard Accessories
•Quick Instruction Guide
•9V Alkaline Battery
•3mL Transfer Pipet
•Full Instrument Manual in available from www.pyxis-lab.com.
1.5. Optional Accessories
•Carry Case for SP-395
•1 ppm HST Standard
1.6. Light Shield Cover
Figure 1 Light Shield in the Open Position

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2. Starting SP-395
2.1. Battery Installation
The SP-395 is powered by a 9-volt alkaline battery. Do not use rechargeable nickel cadmium (NiCad) or
lithium batteries. A typical 9V battery lasts for two months and enables about 3000 measurements.
When the battery capacity is critically low, the SP-395 will display a LOW BATTERY warning for 5
seconds and then automatically turn off.
Replace the battery to resume operation of the SP-395 after the battery warning. The SP-395 will
automatically turn on in the measurement mode after new battery installation.
The SP-395 battery compartment, shown in Figure 2, is on the back side of the instrument.
Install battery as follows:
1. Remove the battery compartment cover by loosening two screws.
2. Make sure that the smaller circular terminal (positive) of the battery is aligned with the
hexagonal socket (positive) of the battery holder and the hexagonal socket (negative) of the
battery with the circular terminal of the holder. Snap the battery firmly into the battery holder.
3. Replace the battery compartment cover, making sure that the sealing O-ring is lying flat on the
battery holder. To prevent the SP-395 from accidently being turned on due to vibration, please
firmly tighten the two screws.

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Figure 2 Install Battery
2.2. Description of Control Keys
The SP-395 has three keys as shown in Figure 1. The left (<), right (>) and OK keys are used to launch an
action indicated on the screen directly above the keys. Please note that the screen is not a tough screen.
The labels above the keys indicate the function associated with the keys and can change according to
the screen modes.

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Figure 3 Key and Functions
2.3. Turning On/Off SP-395
To turn on the SP-395, press OK momentarily and release.
To turn off the SP-395, press and hold the OK key. Release the OK key when the LCD display turns off
(after about 3 seconds). The SP-395 will turn itself off after 60 seconds without user interaction through
the keys.
3. HST Measurement
3.1. Measurement
When powered on, the SP-395 will be in the measurement (read) mode (see figure 3). The displayed
value is the concentration of HST.
The light shield should be in the closed position in order to measure HST. If only conductivity is
measured, the light shield can be left in the open position.

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The SP-395 does not need to be turned off between measurements of two samples. Rinsing the
measurement cell several times using the 3mL Transfer Pipet to inject sample is recommended.
Note: Make sure that there are no air bubbles in the sample cup while taking readings. It is
recommended to use the 3mL Transfer Pipet to out the rest of the sample from the
sampling cup.
3.2. High Color and Turbidity Warning
The SP-395 has extra channels to measure sample turbidity and color to automatically compensate
sample color and turbidity interference. If sample turbidity and color values determined are too high, a
warning will be displayed. In such a case, the user should filter the sample for HST measurement.

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4. Calibration
Calibration SP-395 requires deionized water and a standard solution. HST calibration uses the 1 ppm or
2 ppm HST standard solution.
To calibrate HST, first select the HST measurement mode. Press the Calib key (<) to start the calibration
procedure. Rinse the sample cell with deionized water three times. Fill the cell with deionized water,
close the light shield, and then press ZERO (<) to finish the zero calibration step. If the zero calibration is
successful, a check mark will be displayed next to Press Zero Button. If any abnormal signal is detected,
an error message will be shown.
Rinse the sample cell with the 1 ppm HST standard. Fill the sample cell with the 1 ppm standard, close
the light shield, and press Slope to finish the slope calibration step. If the zero calibration is successful, a
check mark will be displayed next to Press Zero Button. If any abnormal signal is detected, an error
message will be shown.
Figure 4

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Figure 5
Figure 6

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5. Device Information and Diagnosis
The device information is shown when the Info labeled OK key in the measurement mode is pressed
momentarily (Figure 3). The screen contains the device serial number, software version, and hardware
version (Figure 7). The battery life as a percentage and the standard that were used in the last
calibration is also shown.
Press the diagnosis labeled key to switch to the diagnosis screen where raw measurement data are
displayed (Figure 8). The information has no use for normal operation. Please provide an image of both
the device information screen and the diagnosis screen when you contact Pyxis (servic[email protected]m)
for troubleshooting your device.
6. Wireless Connection
The Pyxis SP-395 can be connected to a smart phone or a computer via WIFI or Bluetooth for upgrading
the device software. The SP-395 can be wirelessly paired with other Pyxis devices for exchanging data.
In the normal operation modes, the wireless function is turned off. If you want to explore the SP-395
wireless functions, please contact Pyxis Lab Inc.

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7. How to Clean SP-395
Soak the sampling cup of the SP-395 meter with handheld cleaning solution for 30 minutes. Rinse the
SP-395 sampling cup with distilled water, wipe down sampling cup with Q-tip, rinse with distilled water
once again, and then check for the flashing blue light inside the sampling cup of the SP-395 meter. If the
surface is not entirely clean, continue to soak the SP-395 meter sampling cup for an additional 30
minutes. Pyxis Lab Handheld Cleaning Solution can be purchased at our online Estore/Catalog:
https://pyxis-lab.com/product/handheld-device-cleaning-kit/.
Video on how to clean handheld meters: https://www.youtube.com/watch?v=OJDnCOjw7-M.