Qiagen EZ1 Advanced XL User manual

Sample to Insight__
November 2017
EZ1®Advanced XL
User Manual
9001874
QIAGEN GmbH
QIAGEN Strasse 1
40724 Hilden
GERMANY
R2
110
8598

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Contents
1Introduction .............................................................................................................6
1.1 General information........................................................................................6
1.1.1 Technical assistance................................................................................6
1.1.2 Policy statement......................................................................................6
1.1.3 Version management ..............................................................................6
1.2 Intended use of EZ1 Advanced XL ....................................................................7
1.3 Requirements for EZ1 Advanced XL users ..........................................................7
2Safety Information ....................................................................................................8
2.1 Proper use .....................................................................................................8
2.2 Electrical safety ............................................................................................10
2.3 Environment.................................................................................................11
2.3.1 Operating conditions ............................................................................11
2.4Waste disposal ............................................................................................11
2.5 Biological safety...........................................................................................12
2.5.1 Samples ..............................................................................................12
2.6 Chemicals....................................................................................................12
2.6.1 Toxic fumes..........................................................................................13
2.7 Mechanical hazards .....................................................................................13
2.8 Heat hazard ................................................................................................13
2.9 UV radiation ................................................................................................13
2.10 Maintenance safety ..................................................................................14
2.11 Symbols on the EZ1 Advanced XL instrument ...............................................16
2.11.1 Location of symbols ..............................................................................18
3General Description ...............................................................................................19
3.1 External features of the EZ1 Advanced XL........................................................20
3.1.1 EZ1 Advanced XL door .........................................................................20
3.1.2 Control panel.......................................................................................20
3.1.3 EZ1 Advanced XL card slot ....................................................................21
3.1.4 Status LEDs ..........................................................................................22

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3.1.5 Connector panel...................................................................................22
3.2 Internal features of the EZ1 Advanced XL.........................................................23
3.2.1 Worktable ...........................................................................................23
3.2.2 Pipettor head .......................................................................................26
3.2.3 UV lamps.............................................................................................27
4Installation Procedures ............................................................................................29
4.1 Site requirements..........................................................................................29
4.2 AC power connection ...................................................................................29
4.2.1Power requirements ..............................................................................29
4.2.2 Grounding requirements........................................................................29
4.2.3 Installation of AC power cord ................................................................30
4.3 Hardware installation....................................................................................31
4.3.1 Unpacking the EZ1 Advanced XL ...........................................................31
4.3.2 Installing the EZ1 Advanced XL ..............................................................35
4.3.3 Configuring and setting up the EZ1 Advanced XL .....................................36
4.3.4 Installing the printer ..............................................................................37
4.3.5 Installing the bar code reader ................................................................37
4.4 Installing the PC software...............................................................................38
4.4.1 System requirements .............................................................................38
4.4.2 Installing the driver of the USB–RS-232 converter......................................39
4.4.3 Installing the EZ1 Advanced Communicator software................................41
4.5 Installing multiple EZ1 Advanced XL instruments ...............................................42
4.6 Transportation and removal of the EZ1 Advanced XL ........................................44
5General Operation.................................................................................................45
5.1 Overview ....................................................................................................45
5.2 Inserting and removing the EZ1 Advanced XL card ...........................................45
5.2.1 Inserting the EZ1 Advanced XL card........................................................46
5.2.2 Removing the EZ1 Advanced XL card......................................................47
5.3 Switching the EZ1 Advanced XL on and off .....................................................48
5.3.1 Switching on the EZ1 Advanced XL.........................................................48
5.3.2 Switching off the EZ1 Advanced XL ........................................................48

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5.4 Opening and closing the EZ1 Advanced XL door .............................................49
5.4.1 Opening the EZ1 Advanced XL door.......................................................49
5.4.2 Closing the EZ1 Advanced XL door ........................................................49
5.5 Starting and stopping a protocol run...............................................................50
5.5.1 Starting a protocol run ..........................................................................50
5.5.2 Stopping a protocol run ........................................................................51
5.6 Setting up the worktable................................................................................52
5.6.1 Removing and replacing the tray............................................................52
5.6.2 Loading the reagent cartridges...............................................................53
5.6.3 Loading the elution tubes, filter-tips and sample tubes................................55
5.7 Using the UV lamps ......................................................................................56
5.7.1 Switching on the UV lamps ....................................................................56
5.7.2 Switching off the UV lamps....................................................................58
5.7.3 Lifetime reminder of the UV lamps...........................................................58
5.7.4 UV lamps ignition error .........................................................................58
5.8 Manual operation.........................................................................................59
5.8.1 Manual operation “home axis” ..............................................................59
5.8.2 Manual operation “return tip” ................................................................60
5.8.3 Manual operation “clean” .....................................................................60
5.8.4 Manual operation “resend” ...................................................................61
5.9 Test function.................................................................................................62
5.9.1 Test axis ..............................................................................................62
5.9.2 Test heating block.................................................................................64
5.9.3 Test serial port......................................................................................64
5.9.4 Test version..........................................................................................66
5.10 System setup ............................................................................................67
5.10.1 Setting the date ....................................................................................67
5.10.2 Setting the time ....................................................................................67
5.10.3 Setting the serial port ............................................................................68
5.10.4 Setting the annual maintenance reminder ................................................68
5.11 Using the bar code reader.........................................................................69

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5.12 Using the external printer...........................................................................69
5.13 Generating a report file.............................................................................70
5.14 Using the EZ1 Advanced Communicator software ........................................73
5.14.1 User interface.......................................................................................73
5.14.2 Report file in pdf format.........................................................................76
5.15 Using multiple EZ1 Advanced XL instruments................................................76
5.16 Evaluating pipetting accuracy ....................................................................76
6Maintenance .........................................................................................................77
6.1 Regular maintenance procedure .....................................................................78
6.2 Daily maintenance procedure ........................................................................81
6.3 Weekly maintenance procedure.....................................................................82
6.4 Reagents for decontamination ........................................................................83
7Troubleshooting .....................................................................................................85
7.1 EZ1 Advanced XL door errors ........................................................................85
7.2 Other errors.................................................................................................85
7.3 Error codes ..................................................................................................86
8Glossary ...............................................................................................................88
Appendix A .....................................................................................................................90
Technical data .......................................................................................................90
Operating conditions.........................................................................................90
Transportation conditions ...................................................................................90
Storage conditions ............................................................................................91
Mechanical data and hardware features .............................................................91
Waste Electrical and Electronic Equipment (WEEE) ....................................................93
FCC Declaration ....................................................................................................94
Declaration of Conformity .......................................................................................95
Appendix B .....................................................................................................................96
Liability clause .......................................................................................................96
Index ..............................................................................................................................97

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1Introduction
Thank you for choosing the EZ1 Advanced XL. We are confident it will become an integral part of
your laboratory.
Before using the instrument, it is essential to read this manual carefully and to pay particular
attention to any advice it contains concerning hazards that may arise from use of this instrument.
1.1 General information
1.1.1 Technical assistance
At QIAGEN, we pride ourselves on the quality and availability of our technical support. Our
Technical Services Departments are staffed by experienced scientists with extensive practical and
theoretical expertise in molecular biology and the use of QIAGEN®products. If you have any
questions or experience any difficulties regarding the EZ1 Advanced XL instrument or QIAGEN
products in general, do not hesitate to contact us.
QIAGEN customers are a major source of information regarding advanced or specialized uses of
our products. This information is helpful to other scientists as well as to the researchers at QIAGEN.
We therefore encourage you to contact us if you have any suggestions about product performance
or new applications and techniques.
For technical assistance, contact QIAGEN Technical Services.
For up-to-date information about the EZ1 Advanced XL instrument, visit www.qiagen.com.
1.1.2 Policy statement
It is the policy of QIAGEN to improve products as new techniques and components become
available. QIAGEN reserves the right to change the specifications of products at any time.
1.1.3 Version management
This document is the EZ1 Advanced XL User Manual, revision R2.

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1.2 Intended use of EZ1 Advanced XL
The EZ1 Advanced XL is designed to perform automated isolation and purification of nucleic acids.
It is intended to be used only in combination with QIAGEN kits indicated for use with the EZ1
Advanced XL instrument for the applications described in the kit handbooks.
The system is intended for use by professional users, such as technicians and physicians trained in
molecular biological techniques and the operation of the EZ1 Advanced XL.
1.3 Requirements for EZ1 Advanced XL users
This table covers the general level of competence and training necessary for transportation,
installation, use, maintenance and servicing of the EZ1 Advanced XL
Type of task) Personnel Training and experience
Delivery No special requirements No special requirements
Installation Laboratory technicians or
equivalent
Appropriately trained and
experienced personnel familiar
with use of computers and
automation in general
Routine use (running
protocols)
Laboratory technicians or
equivalent
Professional users, such as
technicians and physicians,
trained in molecular biology
techniques
Routine maintenance Laboratory technicians or
equivalent
Professional users, such as
technicians and physicians,
trained in molecular biology
techniques
Servicing and annual
maintenance
QIAGEN Field Service
Specialists only
Regularly trained, certified,
and authorized by QIAGEN

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2Safety Information
This user manual contains information about warnings and cautions that must be followed by the
user to ensure safe operation of the EZ1 Advanced XL instrument and to maintain the instrument in
a safe condition.
Possible hazards that could harm the user or result in damage to the instrument are clearly stated
at the appropriate places throughout this user manual.
If the equipment is used in a manner not specified by the manufacturer, the protection provided by
the equipment may be impaired.
Check the validity of reagents and disposables as per each instruction or caution of use.
The following safety conventions are used throughout this user manual.
WARNING
The term WARNING is used to inform you about situations that could result in
personal injury to other persons.
Details about these circumstances are given in a box like this one.
CAUTION
The term CAUTION
is used to inform you about situations that could result in
damage to an instrument or other equipment.
Details about these circumstances are given in a box like this one.
The advice given in this manual is intended to supplement, not supersede, the normal safety
requirements prevailing in the user’s country.
2.1 Proper use
The EZ1 Advanced XL must only be operated by qualified personnel who have been appropriately
trained.
Servicing of the EZ1 Advanced XL must only be performed by QIAGEN Field Service Specialists.
WARNING/
CAUTION
Risk of personal injury and material damage
Improper use of the EZ1 Advanced XL may cause personal injuries or
damage to the instrument.

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CAUTION Damage to the instrument
Only use QIAGEN EZ1 Advanced XL cards with the EZ1 Advanced XL.
Other EZ1 cards cannot be used with the EZ1 Advanced XL.
CAUTION Damage to the instrument
The EZ1 Advanced XL card is an integrated part of the system. Ensure that the
EZ1 Advanced XL is always switched off before you insert or remove the EZ1
Advanced XL card.
CAUTION
Damage to the instrument
Avoid spilling water or chemicals onto the EZ1 Advanced XL. Instrument
damage caused by water or chemical spillage will void your warranty.
Perform the maintenance as described in Section 6. QIAGEN charges for repairs that are required
due to incorrect maintenance.
Note: Do not place items on top of the EZ1 Advanced XL instrument.
WARNING Risk of personal injury
Two people must carry the instrument. Use the handle attached to the box to lift
the EZ1 Advanced XL. After the EZ1 Advanced XL has been unpacked, 2 people
mus
t lift the instrument. Lift the instrument by placing your hands underneath the
bottom of the instrument.
Note: In case of emergency, switch off the EZ1 Advanced XL instrument and unplug the power cord
from the power outlet.
CAUTION
Damage to the instrument
Ensure that the EZ1 Advanced XL is switched off before you manually move the
mechanical components of the instrument.

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2.2 Electrical safety
Note: If operation of the instrument is interrupted in any way (e.g., due to interruption of the power
supply or a mechanical error), first switch off the EZ1 Advanced XL instrument, then disconnect the
electrical cord from the power supply and contact QIAGEN Technical Services.
WARNING Electrical hazard
Any interruption of the protective conductor (earth/ground lead) inside or
outside the instrument or disconnection of the protective conductor terminal is
likely to make the instrument dangerous. Intentional interruption is prohibited.
Lethal voltages inside the instrument
When the instrument is connected to line power, terminals may be live. Opening
covers or removing parts is likely to expose live parts.
When working with the EZ1 Advanced XL instrument:
The line power cord must be connected to a line power outlet that has a protective conductor
(earth/ground).
Do not adjust or replace internal parts of the instrument.
Do not operate the instrument with any covers or parts removed.
If liquid has spilled inside the instrument, switch off the instrument, disconnect it from the
power outlet, and contact QIAGEN Technical Services.
The instrument shall be installed in a way that the power cord is accessible.
If the EZ1 Advanced XL instrument becomes electrically unsafe, prevent other personnel from
operating it, and contact QIAGEN Technical Services.
The instruments may be electrically unsafe when:
The EZ1 Advanced XL or the line power cord appears to be damaged.
The EZ1 Advanced XL has been stored under unfavorable conditions for a prolonged period.
The EZ1 Advanced XL has been subjected to severe transport stresses.
Liquids have come into direct contact with electrical components of the EZ1 Advanced XL.
The power cord has been exchanged with a non-official power cord.
WARNING
Electrical hazard
Do not touch the EZ1 Advanced XL with wet hands.