Qiagen TissueLyser II User manual

Sample to Insight__
May 2016
TissueLyser II
User Manual
For disruption of up to 48 or 192 biological
samples

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Contents
Safety Information ..............................................................................................................5
Proper use...............................................................................................................5
Electrical safety........................................................................................................6
Environment.............................................................................................................7
Operating conditions...........................................................................................7
Biological safety ......................................................................................................7
Samples.............................................................................................................7
Chemicals..........................................................................................................8
Toxic fumes ........................................................................................................8
Waste disposal........................................................................................................9
Mechanical hazards.................................................................................................9
Translations of warnings and cautions ........................................................................9
Introduction .....................................................................................................................15
About this user manual ...........................................................................................15
Intended use of the TissueLyser II ..............................................................................15
Damage to the clamps of the TissueLyser II ...........................................................16
Requirements for TissueLyser II users ....................................................................16
General Description..........................................................................................................17
TissueLyser II principle.............................................................................................17
Features of the TissueLyser II system ..........................................................................18
TissueLyser II.....................................................................................................18
TissueLyser Adapter Set 2 x 24...........................................................................20
TissueLyser Adapter Set 2 x 96...........................................................................21
Grinding Jar Set, S. Steel ...................................................................................22
Grinding Jar Set, Teflon®...................................................................................22
TissueLyser Single-Bead Dispensers .....................................................................23
TissueLyser Bead Dispensers, 96-Well .................................................................24
Beads..............................................................................................................25
Applications ..........................................................................................................25

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Installation Procedures.......................................................................................................26
Unpacking the TissueLyser II ....................................................................................26
Installation requirements..........................................................................................26
Removal of the transport security device...............................................................26
Site requirements ..............................................................................................27
Power requirements...........................................................................................28
Grounding requirements ....................................................................................28
Transporting and repacking the TissueLyser II ............................................................28
Installation of a TissueLyser Adapter Set or Grinding Jar Set ........................................29
Replacing the fuses.................................................................................................30
Operating Procedures.......................................................................................................32
Sample disruption ..................................................................................................32
Storing disruption parameters..................................................................................33
Displaying the accumulated operating time ...............................................................34
Displaying the software version................................................................................34
Maintenance Procedures ...................................................................................................35
Servicing..........................................................................................................35
Regular maintenance..............................................................................................35
Cleaning agents ...............................................................................................36
Regular safety checks .............................................................................................37
Troubleshooting Guide......................................................................................................39
Error codes displayed by the TissueLyser II ................................................................39
Appendix A.....................................................................................................................40
Technical data .......................................................................................................40
Environmental conditions.........................................................................................40
Operating conditions.........................................................................................40
Transportation conditions ...................................................................................40
Storage conditions ............................................................................................40
Mechanical data...............................................................................................40
Waste Electrical and Electronic Equipment (WEEE) ....................................................41
Declaration of Conformity .......................................................................................43

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Appendix B .....................................................................................................................44
Liability clause .......................................................................................................44
Notes..............................................................................................................45
Notes..............................................................................................................46

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Safety Information
Before using the TissueLyser II system, it is essential that you read this user manual carefully and
pay particular attention to the safety information. The instructions and safety information in the
user manual must be followed to ensure safe operation of the TissueLyser II and to maintain the
TissueLyser II in a safe condition.
The following types of safety information appear throughout this user manual.
WARNING The term WARNING is used to inform you about situations that could result in personal
injury to you or other persons.
Details about these circumstances are given in a box like this one.
CAUTION The term CAUTION is used to inform you about situations that could result in damage to the
TissueLyser II or other equipment.
Details about these circumstances are given in a box like this one.
The advice given in this manual is intended to supplement, not supersede, the normal safety
requirements prevailing in the user’s country.
Proper use
WARNING Risk of personal injury and material damage (W1)
Improper use of the TissueLyser II may cause personal injuries or damage to the instrument.
Do not attempt to move the TissueLyser II during operation.
The TissueLyser II should only be operated by qualified personnel who have been
appropriately trained.
Servicing of the TissueLyser II should only be performed by QIAGEN or an authorized agent
(service technicians). Do not carry out any modifications of the instrument.
QIAGEN shall not be held responsible for any injuries or damages resulting from
nonobservance of the instructions and safety information in this user manual.
The TissueLyser II should only be used for the applications described in the TissueLyser Handbook.
The TissueLyser II should only be used in combination with accessories approved by QIAGEN
(see “Features of the TissueLyser II system,” page 18). Do not continue to use the TissueLyser II or
TissueLyser accessory if it is damaged.

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Perform the maintenance as described in “Maintenance Procedures,” page 35. QIAGEN
charges for repairs that are required due to incorrect maintenance.
CAUTION Damage to the instrument (C1)
Use of adapters and plasticware other than those described in this user manual may lead to
damage to the clamps of the TissueLyser II.
CAUTION Damage to the instrument (C2)
Avoid spilling water or chemicals onto the TissueLyser II. Damage caused by water or
chemical spillage will void your warranty.
In case of emergency, switch off the TissueLyser II at the power switch and unplug the power cord
from the power outlet.
Electrical safety
WARNING Electrical hazard (W2)
Any interruption of the protective conductor (earth/ground lead) inside or outside the
TissueLyser II or disconnection of the protective conductor terminal is likely to make the
TissueLyser II dangerous.
Intentional interruption is prohibited.
Lethal voltages inside the TissueLyser II
When the TissueLyser II is connected to line power, terminals may be live, and opening the
TissueLyser II or removing parts is likely to expose live parts.
To ensure satisfactory and safe operation of the TissueLyser II, follow the advice below:
The line power cord must be connected to a line power outlet that has a protective
conductor (earth/ground).
Do not adjust or replace internal parts of the TissueLyser II.
Do not operate the TissueLyser II with any covers or parts removed.
If liquid has spilled inside the TissueLyser II, switch off the TissueLyser II, disconnect it
from the power outlet, and contact QIAGEN Technical Services.
If the TissueLyser II becomes electrically unsafe, prevent other personnel from operating
it, and contact QIAGEN Technical Services; the TissueLyser II may be electrically unsafe
when:

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It or the line power cord appears to be damaged.
It has been stored under unfavorable conditions for a prolonged period.
It has been subjected to severe transport stresses.
If the TissueLyser II is not in use, switch it off at the power switch. If the TissueLyser II will not be
used for a prolonged period of time, unplug the power cord from the power outlet.
Environment
Operating conditions
WARNING Explosive atmosphere (W3)
The TissueLyser II is not designed for use in an explosive atmosphere.
WARNING Risk of explosion (W4)
The TissueLyser II is intended for use with reagents and substances supplied with QIAGEN
kits. Use of other reagents and substances may lead to fire or explosion.
CAUTION Damage to the instrument (C3)
Direct sunlight may bleach parts of the instrument and cause damage to plastic parts. The
TissueLyser II must be located out of direct sunlight.
Biological safety
Specimens and reagents containing materials from humans should be treated as potentially
infectious. Use safe laboratory procedures as outlined in publications such as Biosafety in
Microbiological and Biomedical Laboratories, HHS (www.cdc.gov/od/ohs/biosfty/biosfty.htm).
Samples
Samples may contain infectious agents. You should be aware of the health hazard presented by
such agents and should use, store, and dispose of such samples according to the required safety
regulations.
WARNING Samples containing infectious agents (W5)
Some samples used with the TissueLyser II may contain infectious agents. Handle such
samples with the greatest of care and in accordance with the required safety regulations.
Always wear safety glasses, 2 pairs of gloves, and a lab coat.

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The responsible body (e.g., laboratory manager) must take the necessary precautions to
ensure that the surrounding workplace is safe, and that TissueLyser II operators are suitably
trained and not exposed to hazardous levels of infectious agents as defined in the
applicable Safety Data Sheets (SDSs) or OSHA,* ACGIH,†or COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance with all national, state,
and local health and safety regulations and laws.
*OSHA: Occupational Safety and Health Administration (United States of America).
†ACGIH: American Conference of Government Industrial Hygienists (United States of
America).
‡COSHH: Control of Substances Hazardous to Health (United Kingdom).
Chemicals
WARNING Hazardous chemicals (W6)
Some chemicals used with the TissueLyser II may be hazardous or may become hazardous
after completion of the sample disruption process.
Always wear safety glasses, gloves, and a lab coat.
The responsible body (e.g., laboratory manager) must take the necessary precautions to
ensure that the surrounding workplace is safe and that TissueLyser II operators are not
exposed to hazardous levels of toxic substances (chemical or biological) as defined in the
applicable Safety Data Sheets (SDSs) or OSHA,* ACGIH,†or COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance with all national, state,
and local health and safety regulations and laws.
*OSHA: Occupational Safety and Health Administration (United States of America).
† ACGIH: American Conference of Government Industrial Hygienists (United States of
America).
‡COSHH: Control of Substances Hazardous to Health (United Kingdom).
Toxic fumes
If working with volatile solvents, toxic substances, etc., you must provide an efficient laboratory
ventilation system to remove vapors that may be produced.

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WARNING Toxic fumes (W7)
Do not use bleach to clean or disinfect used labware. Bleach in contact with salts from the
buffers used can produce toxic fumes.
Waste disposal
Used labware, such as sample tubes, may contain hazardous chemicals or infectious agents from
the sample disruption process. Such wastes must be collected and disposed of properly according
to local safety regulations. For information on how to dispose of the TissueLyser II, see Appendix
A (page 41).
Mechanical hazards
WARNING Moving parts (W8)
Do not leave the TissueLyser II unattended during operation.
To ensure safety, the TissueLyer II will not operate until the transparent hood is closed.
WARNING Moving parts (W9)
Damage or personal injury can occur if the TissueLyser Adapter Set or Grinding Jar Set is
not securely attached.
Ensure that both adapters or grinding jars are of similar weight. Do not operate the
TissueLyser II with only a single adapter or grinding jar installed.
Translations of warnings and cautions
This subsection contains translations of the warnings and cautions used in this user manual. Each
warning or caution has a reference number in square brackets at the top right of its box.
WARNING The term WARNING is used to inform you about situations that could results in personal
injury to you or other persons.
Details about these circumstances are given in a boxlike this one.
DE WARNING (WARNUNG)
Warnung weist auf Situationen und Umstände hin, die zu einer Verletzung des Benutzers
oder anderer Personen führen können.
Nähere Angaben zu der Art der Gefährdung und der Vermeidung solcher Situationen
werden in einem Textfeld wir diesem neben der Warnung gemacht.

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FR WARNING (DANGER)
La formule WARNING (DANGER) est utilisée pour avertir des situations pouvant
occasionner des dommages corporels à l’utilisateur ou à d’autre personnes. Les détails sur
ces circonstances sont données dans un encadré semblable a celui-ci.
WARNING Risk o
f
personal injury and material damage (W1)
Improper use of the TissueLyser II may cause personal injuries or damage to the instrument.
Do not attempt to move the TissueLyser II during operation.
The TissueLyser II should only be operated by qualified personnel who have been
appropriately trained.
Servicing of the TissueLyser II should only be performed by QIAGEN or an authorized agent
(service technicians). Do not carry out any modifications of the instrument.
QIAGEN shall not be held responsible for any injuries or damages resulting from
nonobservance of the instructions and safety information in this user manual.
DE
V
erletzungsgefahr und Gefahr der Beschädigung des Geräts
Die unsachgemäße Bedienung des TissueLyser II kann zu einer Verletzung des Benutzers
oder zur Beschädigung des Geräts führen. Der TissueLyser II darf auf keinen Fall während
des Betriebs bewegt werden.
Die Bedienung des TissueLyser II darf nur durch qualifiziertes, entsprechend geschultes
Personal erfolgen.
Wartungsarbeiten am TissueLyser II sollten nur durch QIAGEN oder durch autorisierte
Vertreter (Servicetechniker) erfolgen. Nehmen Sie keine eigenhändigen Veränderungen am
Gerät vor.
Für Sach- und Personenschäden, die durch Nichtbeachtung der Anweisungen und
Sicherheitshinweise in diesem Handbuch entstehen, schließt QIAGEN Schadensansprüche
in jeglicher Form aus.
FR Risque d’accident corporel et de détérioration du matériel
L’utilisation inappropriée du TissueLyser II peut provoquer des accidents corporels ou une
détérioration de l’appareil. Ne pas essayer de déplacer le TissueLyser II pendant qu’il est en
marche.
Le TissueLyser II ne doit être utilisé que par du personnel qualifié ayant été convenablement
formé.
L’entretien du TissueLyser II doit être effectué exclusivement par QIAGEN ou un agent
autorisé (techniciens d’entretien). Ne pas effectuer de modifications quelconques sur
l’appareil.
QIAGEN ne sera pas responsable des accidents ou des détériorations dus au non-respect
des instructions et des informations de sécurité mentionnées dans ce manuel d’utilisation.

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WARNING Electrical hazard (W2)
Any interruption of the protective conductor (earth/ground lead) inside or outside the
TissueLyser II or disconnection of the protective conductor terminal is likely to make the
TissueLyser II dangerous.
Intentional interruption is prohibited.
Lethal voltages inside the TissueLyser II
When the TissueLyser II is connected to line power, terminals may be live, and opening the
TissueLyser II or removing parts is likely to expose live parts.
DE Gefahr durch Stromschlag
Jede Unterbrechung des Schutzleiters (Erdungs- bzw. Masseleiter) im TissueLyser II oder
außerhalb des Geräts und jede Abtrennung des Schutzleiters am Anschluss der Netzleitung
erhöht die Gefahr eines Stromschlags.
Eine absichtliche Unterbrechung der Schutzleiterverbindung ist verboten.
Gefährliche Spannung im TissueLyser II
Wenn der TissueLyser II an die Stromversorgung angeschlossen ist, können die
Anschlussstellen spannungsführend sein. Durch das Öffnen des TissueLyser II oder das
Entfernen von Teilen können spannungsführende Komponenten freigelegt werden.
FR Danger électrique
Toute interruption du conducteur de protection (conducteur de terre/de masse) à l’intérieur
ou à l’extérieur du TissueLyser II ou toute déconnexion de la borne du conducteur de
protection est susceptible de rendre le TissueLyser II dangereux.
Toute interruption intentionnelle est interdite.
Tensions mortelles à l’intérieur du TissueLyser II
Lorsque le TissueLyser II est relié à l’alimentation, les bornes peuvent être sous tension et
l’ouverture du TissueLyser II ou le retrait de pièces risque d’exposer des éléments sous
tension.
WARNING Explosive atmosphere (W3)
The TissueLyser II is not designed for use in an explosive atmosphere.
DE Explosionsfähige Atmosphäre
Der TissueLyser II ist nicht für den Betrieb in einer explosionsfähigen Atmosphäre ausgelegt.
FR
A
tmosphère explosive
Le TissueLyser II n’est pas conçu pour être utilisé dans une atmosphère explosive.
WARNING Risk of explosion (W4)
The TissueLyser II is intended for use with reagents and substances supplied with QIAGEN
kits. Use of other reagents and substances may lead to fire or explosion.
DE Explosionsgefah
r
Der TissueLyser II ist ausgelegt für den Betrieb zusammen mit den Reagenzien und
Substanzen, die im Lieferumfang der QIAGEN-Kits enthalten sind. Die Verwendung anderer
Reagenzien und Substanzen kann unter Umständen einen Brand oder eine Explosion
auslösen.

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FR Risque d’explosion
Le TissueLyser II est conçu pour être utilisé avec les réactifs et substances fournis avec les kits
QIAGEN. L’utilisation d’autres réactifs et substances présente des risques d’incendie ou
d’explosion.
WARNING
Samples containing infectious agents (W5)
Some samples used with the TissueLyser II may contain infectious agents. Handle such
samples with the greatest of care and in accordance with the required safety regulations.
Always wear safety glasses, 2 pairs of gloves, and a lab coat.
The responsible body (e.g., laboratory manager) must take the necessary precautions to
ensure that the surrounding workplace is safe, and that TissueLyser II operators are suitably
trained and not exposed to hazardous levels of infectious agents as defined in the
applicable Safety Data Sheets (SDSs) or OSHA,* ACGIH,†or COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance with all national, state,
and local health and safety regulations and laws.
DE Proben, die Infektionserreger enthalten
Einige der mit dem TissueLyser II verwendeten Proben können Infektionserreger enthalten.
Gehen Sie beim Umgang mit derartigen Proben mit der größtmöglichen Vorsicht und
gemäß den erforderlichen Sicherheitsbestimmungen vor.
Tragen Sie immer eine Schutzbrille, zwei Paar Laborhandschuhe und einen Laborkittel.
Die verantwortliche Person (z. B. der Laborleiter) muss alle erforderlichen
Vorsichtsmaßnahmen treffen, um sicherzustellen, dass die unmittelbare Umgebung des
Arbeitsplatzes sicher ist und die Bediener des Geräts ausreichend geschult sind. Außerdem
dürfen die Grenzwerte in Bezug auf Infektionserreger, die in den entsprechenden
Sicherheitsdatenblättern (SDS) oder den Vorschriften der OSHA*, ACGIH†oder COSHH‡
festgelegt sind, nicht überschritten werden.
Beim Betrieb eines Abzugs und bei der Entsorgung von Abfallstoffen müssen alle
Bestimmungen und Gesetze zu Gesundheitsschutz und Sicherheit am Arbeitsplatz auf
Bundesebene, Landesebene und kommunaler Ebene eingehalten werden.
FR
É
chantillons contenant des agents infectieu
x
Certains échantillons utilisés avec le TissueLyser II peuvent contenir des agents infectieux.
Manipulez ces échantillons avec la plus grande précaution et conformément aux règles de
sécurité nécessaires.
Portez toujours des lunettes de protection, 2 paires de gants et une blouse de laboratoire.
La personne responsable (par exemple, le directeur du laboratoire) doit prendre les
précautions nécessaires afin de garantir que le lieu de travail environnant est sûr et que les
opérateurs du TissueLyser II sont convenablement formés et ne sont pas exposés à des
niveaux dangereux d’agents infectieux comme cela est défini dans les fiches techniques
santé-sécurité (SDS) ou dans les documents de l’OSHA, de l’ACGIH,†ou du COSHH‡
applicables.
L’évacuation des vapeurs et la mise au rebut des déchets doivent être effectuées
conformément à toutes les réglementations et lois nationales, régionales et locales relatives
à la santé et à la sécurité.

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WARNING
Hazardous chemicals (W6)
Some chemicals used with the TissueLyser II may be hazardous or may become hazardous
after completion of the sample disruption process.
Always wear safety glasses, gloves, and a lab coat.
The responsible body (e.g., laboratory manager) must take the necessary precautions to
ensure that the surrounding workplace is safe and that TissueLyser II operators are not
exposed to hazardous levels of toxic substances (chemical or biological) as defined in the
applicable Safety Data Sheets (SDSs) or OSHA,* ACGIH,†or COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance with all national, state,
and local health and safety regulations and laws.
DE Gefährliche Chemikalien
Einige der mit dem TissueLyser II verwendeten Chemikalien können unter Umständen
gefährlich sein oder nach Abschluss des Probenaufschlusses gefährlich werden.
Tragen Sie immer eine Schutzbrille, Laborhandschuhe und einen Laborkittel.
Die verantwortliche Person (z. B. der Laborleiter) muss alle erforderlichen
Vorsichtsmaßnahmen treffen, um sicherzustellen, dass die unmittelbare Umgebung des
Arbeitsplatzes sicher ist. Auch dürfen die Grenzwerte in Bezug auf infektiöse Erreger, die in
den entsprechenden Sicherheitsdatenblättern (SDS) oder den Vorschriften der OSHA*,
ACGIH†oder COSHH‡festgelegt sind, nicht überschritten werden.
Beim Betrieb eines Abzugs und bei der Entsorgung von Abfallstoffen müssen alle
Bestimmungen und Gesetze zu Gesundheitsschutz und Sicherheit am Arbeitsplatz auf
Bundesebene, Landesebene und kommunaler Ebene eingehalten werden.
FR Produits chimiques dangereu
x
Certains produits chimiques utilisés avec le TissueLyser II peuvent être dangereux ou le
devenir après l’exécution du processus de broyage d’échantillon.
Toujours porter des lunettes de protection, des gants et une blouse de laboratoire.
La personne responsable (par exemple, le directeur du laboratoire) doit prendre les
précautions nécessaires afin de garantir que le lieu de travail environnant est sûr et que les
opérateurs du TissueLyser II ne sont pas exposés à des niveaux dangereux de substances
(chimiques ou biologiques) toxiques comme cela est défini dans les fiches techniques santé-
sécurité (MSDS) ou dans les documents de l’OSHA, de l’ACGIH,†ou du COSHH‡
applicables.
L’évacuation des vapeurs et la mise au rebut des déchets doivent être effectuées
conformément à toutes les réglementations et lois nationales, régionales et locales relatives
à la santé et à la sécurité.
WARNING
Toxic fumes (W7)
Do not use bleach to clean or disinfect used labware. Bleach in contact with salts from the
buffers used can produce toxic fumes.
DE Giftige Dämpfe
Verwenden Sie zur Reinigung oder Desinfektion von gebrauchten Labormaterialien keine
Chlorbleiche. Die Chlorbleiche kann bei Kontakt mit Salzen aus den verwendeten Puffern
giftige Dämpfe bilden.

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FR
V
apeurs toxiques
N’utilisez pas de javellisant pour nettoyer ou désinfecter le matériel de laboratoire. Le
contact d’un produit à base d’eau de Javel avec des sels provenant des tampons utilisés
peut produire des vapeurs toxiques.
WARNING
Moving parts (W8)
Do not leave the TissueLyser II unattended during operation.
DE Sich bewegende Geräteteile
Lassen Sie den TissueLyser II während des Betriebs nicht unbeaufsichtigt.
FR Pièces mobiles
Ne pas laisser le TissueLyser II sans surveillance lorsqu’il fonctionne.
WARNING
Moving parts (W9)
Damage or personal injury can occur if the TissueLyser Adapter Set or Grinding Jar Set is
not securely attached.
Ensure that both adapters or grinding jars are of similar weight. Do not operate the
TissueLyser II with only a single adapter or grinding jar installed.
DE Sich bewegende Geräteteile
Wenn das TissueLyser Adapter Set oder das Grinding Jar Set nicht sicher montiert ist, kann
es zu Sach- und Personenschäden kommen.
Achten Sie darauf, dass beide Adapter bzw. Mahlbecher etwa gleich viel wiegen.
Betreiben Sie den TissueLyser II nie, wenn nur ein einzelner Adapter oder Mahlbecher
eingebaut ist.
FR Pièces mobiles
La fixation incorrecte du jeu d’adaptateurs TissueLyser Adapter Set ou de l’ensemble de
broyage Grinding Jar Set peut détériorer le matériel ou provoquer des accidents corporels.
S’assurer que les deux adaptateurs ou les deux cylindres de broyage ont un poids similaire.
Ne pas faire fonctionner le TissueLyser II avec un seul adaptateur ou un seul cylindre de
broyage.

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Introduction
Thank you for choosing the TissueLyser II system. We are confident it will become an integral part
of your laboratory.
Before using the TissueLyser II, it is essential you read this user manual carefully and pay
particular attention to the safety information. The instructions and safety information in the user
manual must be followed to ensure safe operation of the TissueLyser II and to maintain the
TissueLyser II in a safe condition.
About this user manual
This user manual provides information about the TissueLyser II in the following sections:
1. Safety Information
2. Introduction
3. General Description
4. Installation Procedures
5. Operating Procedures
6. Maintenance Procedures
7. Troubleshooting Guide
Appendices
The appendices contain the following:
Technical data
Warranty terms
Intended use of the TissueLyser II
The TissueLyser II is intended for molecular biology applications. This product is neither intended for
the diagnosis, prevention, or treatment of a disease, nor has it been validated for such use either
alone or in combination with other products. Therefore, the performance characteristics of the
product for clinical use (i.e., diagnostic, prognostic, therapeutic, or blood banking) are unknown.
The TissueLyser II is not designed for continuous operation, but for single 8-hour shifts with 30%
operating time. QIAGEN has successfully tested the TissueLyser II with the following adapter sets and
grinding beads:

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TissueLyser Adapter Set 2 x 96, with one 3 mm diameter tungsten carbide bead or one 5 mm
diameter stainless steel bead per collection microtube
TissueLyser Adapter Set 2 x 24, with one 3 mm diameter tungsten carbide bead or one 5 mm
or 7 mm diameter stainless steel bead per microcentrifuge tube
Damage to the clamps of the TissueLyser II
The clamps of the TissueLyser II are designed for use with the following QIAGEN adapters and
consumables:
TissueLyser Adapter Set 2 x 24 (cat. no. 69982) containing standard 2 ml microcentrifuge
tubes
TissueLyser Adapter Set 2 x 96 (cat. no. 69984) containing Collection Microtubes (racked)
(cat. no. 19560) sealed with Collection Microtube Caps (cat. no. 19566)
QIAGEN adapters together with the recommended tubes form a component that is of an optimal
thickness, allowing secure tightening of the clamps of the TissueLyser II without straining the
clamping mechanism.
Damage to the clamps of the TissueLyser II will occur if other components of reduced thickness are
installed. Therefore, the TissueLyser II must not be used with adapters from other suppliers or with
QIAGEN adapters containing tubes not recommended by QIAGEN.*
Requirements for TissueLyser II users
This table covers the general level of competence and training necessary for transportation,
installation, use, maintenance, and servicing of the TissueLyser II.
Task Personnel
Training and experience
Transportation No special requirements No special requirements
Installation, routine use, and
maintenance
Laboratory technicians or
equivalent
Appropriately trained and
experienced personnel
Servicing QIAGEN or an authorized agent
(service technicians)
*Use of the TissueLyser II with non-QIAGEN adapters has been tested by other suppliers (e.g., Roche recommend use of the
TissueLyser II with GS FLX Titanium emPCR Shaker Adapters, LV, which are accessories for the Genome Sequencer FLX
Instrument). Use of such adapters has not been tested by QIAGEN.

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General Description
The TissueLyser II is a flexible bead mill designed for rapid and efficient disruption of up to 48 or
192 samples at the same time. A wide variety of biological samples can be processed, including
human, animal, and plant tissues as well as yeast and bacteria. Each sample is simultaneously
disrupted and homogenized by high-speed shaking with a bead in a sealed tube (bead milling).
TissueLyser II principle
Efficient sample disruption is a prerequisite for nucleic acid and protein purification procedures.
Incomplete sample disruption can lead to significantly reduced yields and can increase the risk of
clogging when using purification columns or magnetic particles. The TissueLyser II thoroughly
disrupts and simultaneously homogenizes biological samples in the presence of lysis buffer. Plant
tissues can alternatively be disrupted and homogenized in the absence of lysis buffer.
Tubes containing sample and bead are placed in a TissueLyser Adapter Set and shaken at a very
high speed, causing the samples to be disrupted and homogenized by the beating and grinding
effect of the beads. The tubes can be discarded after use, which prevents cross-contamination and
provides time savings, as there is no need to clean tubes after disrupting samples. In addition,
there is no cross-contamination during sample disruption as each tube is securely sealed with its
own lid.
The TissueLyser II can also disrupt and homogenize large samples when used in combination with
a Grinding Jar Set. Samples are placed in a 10 ml jar containing a grinding ball and shaken at
high speed.
TissueLyser Adapter Sets, Grinding Jar Sets, and sample tubes can be precooled to minimize
degradation of nucleic acids during sample disruption. To find out which procedures require
precooling, see the TissueLyser Handbook.

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Features of the TissueLyser II system
TissueLyser II
The TissueLyser II provides high-speed shaking with oscillation frequencies of 3–30 Hz (180–
1800 oscillations per minute). Two clamps at the front of the instrument hold either a TissueLyser
Adapter Set or a Grinding Jar Set (Figures 1 and 2). A safety mechanism prevents the instrument
from operating until the transparent hood is lowered over the TissueLyser Adapter Set or Grinding
Jar Set.
Power is switched on and off at the power switch located at the back of the instrument. Oscillation
frequency can be adjusted in steps of 0.1 Hz using a keypad (Figure 3) located on top of the
instrument. Disruption time, from 10 seconds up to a maximum of 99 minutes, can also be set
using the keypad (Figure 3). The disruption parameters for a particular application (i.e.,
oscillation frequency and disruption time) can be stored in the memory of the TissueLyser II. Up to
9 disruption parameters can be stored.
Figure 1. TissueLyser II (closed). The transparent hood is lowered, and the clamps at the front of the instrument are empty.
Note: The maximum oscillation frequency may drop down to 25 Hz, depending on local
fluctuations in the mains voltage.

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Figure 2. TissueLyser II (open). The transparent hood is raised, and a TissueLyser Adapter Set is installed.

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Figure 3. Keypad of the TissueLyser II. A“Memory” display, with “PROG” and “SET” keys for storing disruption parameters. B
“Frequency 1/s” display, with “–” and “+” keys for adjusting oscillation frequency. C “Time min/sec” display, with “–” and
“+” keys for adjusting disruption time. D“START” and “STOP” keys for starting and stopping operation of the TissueLyser II.
TissueLyser Adapter Set 2 x 24
The TissueLyser Adapter Set 2 x 24 (cat. no. 69982) allows disruption of 48 samples in parallel
using standard 2 ml microcentrifuge tubes (e.g., Eppendorf Safe-Lock micro test tubes
* ). The
adapter set contains 2 adapters, with each adapter comprised of a top plate, a bottom plate, and
a tube holder for holding 24 microcentrifuge tubes (Figure 4). Sample disruption can be carried
out at room temperature or after storing the adapter set at –80°C for at least 2 hours. The adapter
set can be cleaned with detergent, microbicides, or up to 96% ethanol. For more information, see
the product sheet supplied with the adapter set.
*Use of other tubes in combination with 7 mm diameter beads may result in the beads getting lodged in the lids of the tubes.
A
B
C
D