Qiagen Hybrid Capture System Multi-Specimen Tube Vortexer... User manual

Sample to Insight__
September 2015
Hybrid Capture®System Multi-Specimen
Tube Vortexer 2 User Manual
6000-5021 (120 V)
6000-5022 (240 V)
QIAGEN
19300 Germantown Road
Germantown, MD
20874
USA
QIAGEN GmbH
QIAGEN Strasse 1
40724 Hilden
GERMANY
1087788EN Rev. 03

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Hybrid Capture System MST Vortexer 2 User Manual 09/2015
Trademarks: QIAGEN®, Sample to Insight®, digene®, HC2®, Hybrid Capture®(QIAGEN Group); DuraSeal™ (Diversified Biotech).
Registered names, trademarks, etc., used in this document, even when not specifically marked as such, are not to be considered unprotected by law.
© 2015 QIAGEN, all rights reserved.

Hybrid Capture System MST Vortexer 2 User Manual 09/2015
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Contents
1Introduction ..........................................................................................................5
1.1 General information....................................................................................5
1.1.1 Technical assistance............................................................................5
1.1.2 Version management..........................................................................5
1.2 Intended use ...............................................................................................5
2Safety Information.................................................................................................6
2.1 Proper use ..................................................................................................7
2.2 Electrical safety ...........................................................................................9
2.3 Environment ...............................................................................................9
2.4 Biological safety ..........................................................................................9
2.5 Waste disposal..........................................................................................10
2.6 Symbols....................................................................................................11
3Installation..........................................................................................................14
3.1 Unpacking................................................................................................14
3.2 Start up.....................................................................................................14
4Functional Description .........................................................................................15
4.1 Operational modes...................................................................................16
4.2 Specimen racks.........................................................................................16
5General Operation..............................................................................................18
5.1 Continuous vortexing.................................................................................18
5.2 Pulsating vortexing ....................................................................................18
6Maintenance.......................................................................................................20

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Hybrid Capture System MST Vortexer 2 User Manual 09/2015
6.1 Cleaning and decontamination..................................................................20
6.2 Regular maintenance ................................................................................21
6.3 Speed calibration ......................................................................................21
6.3.1 Prepare the materials........................................................................21
6.3.2 Secure the specimen rack..................................................................22
6.3.3 Measure the RPM..............................................................................22
6.3.4 Results..............................................................................................23
6.4 Resetting the circuit breaker .......................................................................23
6.5 Mounting feet replacement ........................................................................24
6.6 Servicing...................................................................................................25
7Troubleshooting ..................................................................................................26
8Technical Data....................................................................................................28
8.1 Operating conditions.................................................................................28
8.2 Transport conditions..................................................................................29
8.3 Storage conditions ....................................................................................29
Appendix A – Waste Electrical and Electronic Equipment (WEEE).......................................30
Appendix B – Warranty...................................................................................................31
Ordering Information.....................................................................................................32

Hybrid Capture System MST Vortexer 2 User Manual 09/2015
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1Introduction
The Hybrid Capture System (HCS) Multi-Specimen Tube (MST) Vortexer 2 has been designed
specifically for vortexing specimens secured in either a digene®Specimen Rack or a
Conversion Rack.
Read this user manual before operating the HCS MST Vortexer 2.
1.1 General information
1.1.1 Technical assistance
For technical assistance and more information, please see our Technical Support Center at
www.qiagen.com/TechSupportCenter
or contact QIAGEN Technical Services or a local
distributor.
1.1.2 Version management
This document is Hybrid Capture System Multi-Specimen Tube Vortexer 2 User Manual; see
the front cover of this user manual for document number and revision.
1.2 Intended use
The HCS MST Vortexer 2 is intended for use only in conjunction with digene Hybrid
Capture 2 (HC2®) DNA tests. Use in conjunction with a Conversion Rack or a digene
Specimen Rack in an indoor laboratory setting.

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Hybrid Capture System MST Vortexer 2 User Manual 09/2015
2Safety Information
This manual contains information about warnings and cautions that must be followed by the
user to ensure safe operation of the MST Vortexer 2 and to maintain the instrument in a safe
condition.
WARNING
The term
WARNING
is used to inform you about situations that could
result in personal injury to you or other persons.
Details about these circumstances are provided to avoid personal injury to
you or other persons.
CAUTION
The term
CAUTION
is used to inform you about situations that could
result in damage to the instrument or other equipment.
Details about these circumstances are provided to avoid damage to the
instrument or other equipment.
Before using the instrument, it is essential to read this manual carefully and to pay particular
attention to any details it contains concerning hazards that may arise from the use of the
instrument.
The details given in this manual are intended to supplement, not supersede, the normal
safety requirements prevailing in the user’s country.

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2.1 Proper use
WARNING/
CAUTION
Risk of personal injury and material damage
Improper use of the MST Vortexer
2 may cause personal injuries to the
user or damage to the instrument.
The MST Vortexer 2 must only be operated by qualified personnel who
have been appropriately trained.
WARNING
Risk of personal injury
The MST Vortexer 2 is a heavy instrument. After the MST Vortexer 2 is
unpacked, two people must lift the instrument. Lift the MST Vortexer 2 by
the handles provided.
WARNING
Risk of personal injury
Always wear shatterproof eye protection.

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Hybrid Capture System MST Vortexer 2 User Manual 09/2015
WARNING
Risk of personal injury
To avoid possible pinch hazard, load and unload the specimen rack with
one hand on top of the rack handle. Use the other hand to engage or
disengage the MST Vortexer 2 mechanism clamp.
WARNING
Risk of personal injury
Do not immerse the MST Vortexer 2 in water or pour liquids over the
instrument as electrical shock may occur.
WARNING
Risk of personal injury
Sodium hypochlorite solution is caustic; wear rubber gloves and eye
protection when handling it.
Take the following precautions when operating or working near the MST Vortexer 2:
Make sure that the MST Vortexer 2 is placed on a firm surface of solid construction.
Make sure that the platform and any hardware are secure.
Make sure the specimen rack is secured on the platform on all 4 sides.
Load only one specimen rack at a time.
Do not use solvents and flammables on or near the MST Vortexer 2.
Make sure that the rubber pads remain attached to the MST Vortexer 2 while relocating
the equipment.

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2.2 Electrical safety
Only operate the MST Vortexer 2 with the power cord provided with the instrument. For
satisfactory and safe operation of the MST Vortexer 2, it is essential that the line power cord
is connected to true electrical earth (ground).
2.3 Environment
Place the MST Vortexer 2 on a level, stable, firmly-secured bench top near a grounded
electrical outlet. Allow at least 7.5 cm (3 inches) of clearance on all sides of the instrument
for proper ventilation. While operating, make sure that the vortexer platform does not touch
other objects. Locate it away from other vibration-sensitive instruments, such as an analytical
balance.
Make sure that the MST Vortexer 2 is rated for the proper voltage by checking the right side
panel. Record the serial number, located on the right side panel, in a secure location for
future reference.
2.4 Biological safety
WARNING
Hazardous substances
The products used with this instrument may contain hazardous substances.
When working with chemicals, always wear a suitable lab coat, disposable
gloves and protective goggles. For more information, please consult the
appropriate safety data sheets (SDSs). These are available online in PDF
format at
www.qiagen.com/safety
where you can find, view and print
the SDS for each QIAGEN kit and kit component. For further information
see the instructions for use that comes with the kit.

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Hybrid Capture System MST Vortexer 2 User Manual 09/2015
WARNING
Risk of exposure to hazardous material
Shake hazardous samples only in appropriate containment vessels.
WARNING/
CAUTION
Risk of personal injury and material damage
Consider any laboratory equipment used for research or clinical analysis a
potential biohazard that requires decontamination before reuse.
To dispose of the MST Vortexer 2, follow all national, state and local health and safety
regulation and laws for disposing of laboratory waste. For disposal of Waste Electrical and
Electronic Equipment (WEEE compliance), see “Appendix A – Waste Electrical and Electronic
Equipment (WEEE),” page 30.
2.5 Waste disposal
Waste may contain certain hazardous chemicals or contagious/biohazardous materials and
must be collected and disposed of properly in accordance with all national, state and local
health and safety regulations and laws.

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2.6 Symbols
The following symbols may be found on the instrument, in this user manual, or on labels
associated with the instrument.
Symbol
Location
Description
On the instrument Warning, dangerous voltage
On the instrument General warning sign
Type plate on the
instrument
CE mark for Europe
Type plate on the
instrument
In vitro diagnostic medical device
Type plate on the
instrument
RoHS mark for China (the restriction of the use
of certain hazardous substances in electrical
and electronic equipment)

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Hybrid Capture System MST Vortexer 2 User Manual 09/2015
Symbol
Location
Description
Type plate on the
instrument
Waste Electrical and Electronic Equipment
(WEEE)
Type plate on the
instrument
Serial number
Type plate on the
instrument
Manufacturer
Instrument box label Fragile, handle with care
Instrument box label Consult instructions for use
Type plate on the
instrument
Global Trade Item Number

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Symbol
Location
Description
Front cover of this user
manual
Authorized representative in the European
Community
Front cover of this user
manual
Catalog number

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Hybrid Capture System MST Vortexer 2 User Manual 09/2015
3Installation
3.1 Unpacking
Before using the MST Vortexer 2 for the first time, examine the exterior carton and the
equipment itself for damage. In the event of shipping damage, contact your local QIAGEN
representative or QIAGEN Technical Services.
Carefully unpack the instrument and verify the contents of the package, which should
contain the following components of the instrument:
1 MST Vortexer 2 (base with platform)
1 power cord
If any of these items are missing, contact your local QIAGEN representative or QIAGEN
Technical Services. Save the original packaging until the instrument is operated successfully.
3.2 Start up
Plug the power cord into a grounded wall socket. The motor speed control electronics of the
MST Vortexer 2 depend on a sinusoidal power source. Do not connect the MST Vortexer 2 to
a UPS or other device that produces a stepped or square power waveform.

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4Functional Description
The MST Vortexer 2 is composed of a base (120V or 240V) and a vortexer platform. The
MST Vortexer 2 is constructed of heavy-gauge steel that provides a stable base for steady
operation, even at maximum speed. The vortexer platform can hold up to 4.5 kg (10 lbs) of
weight. The MST Vortexer 2 can only be used in environments from 0–37°C.
The following figure shows the major external components of the instrument.
Base Red lever
Vortexer platform Specimen rack
1
2
3
4
1
2
34
5

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Hybrid Capture System MST Vortexer 2 User Manual 09/2015
All of the operating controls for the MST Vortexer 2 are located on the front panel. The
following figure shows the front panel.
Motor speed dial Pulser toggle switch
Power toggle switch Pulsation frequency dial
4.1 Operational modes
The motor speed of the MST Vortexer 2 varies from 0–1600 revolutions per minute (RPM). It
operates in a circular motion with an orbit of 0.51–0.71 cm (0.20–0.28 inches). The MST
Vortexer 2 has two vortexing modes: continuous or pulsating.
4.2 Specimen racks
The MST Vortexer 2 has been designed to function with either a digene Specimen Rack or a
Conversion Rack. Each specimen rack is engraved with a serial number on the rack and the
lid; when using a specimen rack, the serial numbers of the rack and the lid must match. The
specimen racks are color-coded for easy identification of the specimen rack type.
1
2
1
2 3
4
4
3

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The digene Specimen Rack is blue and is used with specimens collected in approved Hybrid
Capture collection kits, as specified in the associated digene HC2 DNA test instructions for
use.
The Conversion Rack is silver and used with liquid cytology specimens collected in 15 ml
conical tubes. These specimens require processing prior to testing with a digene HC2 DNA
test. The Conversion Rack has a notched corner that orients the rack for proper placement
on the MST Vortexer 2.

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5General Operation
5.1 Continuous vortexing
Note
: The digene HC2 DNA tests only use the continuous vortexing operational mode.
1. Move the red lever to the horizontal position.
2. Position the specimen rack and lid on the vortexer platform until it fits snugly within the
guides. If using a Conversion Rack, place the specimen rack with the notched corner of
the specimen rack in the right-front position of the vortexer platform.
3. To lock the specimen rack in place, push the red handle all the way down to the vertical
position.
4. Turn the motor speed dial clockwise to the applicable setting.
5. Make sure the pulser toggle switch is in the
OFF
position.
6. To begin operation of the MST Vortexer 2, turn the power toggle switch to the
ON
position.
The MST Vortexer 2 speed will be consistent until the power toggle switch is set to
OFF
.
5.2 Pulsating vortexing
1. Move the red lever to the horizontal position.
2. Position the specimen rack and lid on the vortexer platform until it fits snugly within the
guides. If using a Conversion Rack, place the specimen rack with the notched corner of
the specimen rack in the right-front position of the vortexer platform.
3. To lock the specimen rack in place, push the red handle all the way down to the vertical
position.
4. Turn the motor speed dial clockwise to the applicable setting.

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5. Turn the pulser toggle switch to the
ON
position.
6. Adjust the pulsation frequency dial to the applicable setting.
7. To begin operation of the MST Vortexer 2, turn the power toggle switch to the
ON
position.
The MST Vortexer 2 will pulsate until the power toggle switch is set to
OFF
.

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Hybrid Capture System MST Vortexer 2 User Manual 09/2015
6Maintenance
If you have a problem with maintenance of the MST Vortexer 2, contact QIAGEN Technical
Services. QIAGEN charges for repairs that are required due to incorrect maintenance.
6.1 Cleaning and decontamination
WARNING/
CAUTION
Risk of personal injury and material damage
Consider any laboratory equipment used for research or clinical analysis a
potential biohazard that requires decontamination before reuse.
Wear powder-f
ree gloves when handling potentially contaminated
equipment.
Before using any cleaning or decontamination method, except those recommended in this
user manual, check with your local QIAGEN representative or QIAGEN Technical Services to
make sure that the proposed method will not damage the equipment.
WARNING
Risk of personal injury
Sodium hypochlorite solution is caustic; wear rubber gloves and eye
protection when handling it.
To decontaminate the MST Vortexer 2, wipe down exposed surfaces using a cleaning pad
wetted with a solution of 0.5% sodium hypochlorite (NaOCl or bleach). Industrial bleach
contains 10% NaOCl; household bleach contains 5% NaOCl. When using industrial bleach,