Qiagen QIAcube User manual

Sample to Insight__
April 2019
QIAcube®Connect
Safety Instructions and
Quick Start Guide
For use with Q-Base

2
QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
Contents
Introduction................................................................................................................4
Safety Information.......................................................................................................5
Proper use .......................................................................................................5
Electrical safety ................................................................................................8
Environment.....................................................................................................9
Biological safety.............................................................................................10
Chemicals .....................................................................................................11
Toxic fumes ..............................................................................................11
Waste disposal..............................................................................................12
Mechanical hazards.......................................................................................12
Centrifuge ................................................................................................13
Heat hazard ..................................................................................................14
Maintenance safety ........................................................................................15
Radiation safety .............................................................................................16
Translations of Warnings and Cautions.......................................................................17
Warnings......................................................................................................17
Cautions........................................................................................................35
Symbols on the QIAcube Connect .............................................................................. 42
Installation Procedures...............................................................................................47
Site requirements............................................................................................47
Setting up the QIAcube Connect instrument .......................................................47
To set up the QIAcube Connect instrument ...................................................47

QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
3
Connecting the QIAcube Connect via Wi-Fi ................................................. 49
Connecting the QIAcube Connect via LAN cable..........................................49
Connecting the Q-Base ...................................................................................50
Connecting the Q-Base ..............................................................................50
To define WLAN/Wi-Fi (WPAv2 only) setting...............................................51
To define LAN (Static IP and DHCP) setting ..................................................51
Revision History........................................................................................................52

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QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
Introduction
The QIAcube®Connect is designed to perform fully-automated purification of nucleic acids
and proteins in molecular biology applications. The system is intended for use by professional
users trained in molecular biological techniques and the operation of the QIAcube Connect.
The QIAcube Connect is intended to be used only in combination with QIAGEN kits indicated
for use with the QIAcube Connect for the applications described in the kit handbooks.

QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
5
Safety Information
Before using the QIAcube Connect, it is essential that you read this guide carefully and pay
attention to the safety information. The instructions and safety information in this guide must
be followed to ensure safe operation of the instrument and to maintain the instrument in a safe
condition.
WARNING
The term WARNING is used to inform you about situations that
could result in personal injury to you or other persons.
Details about these circumstances are given in a box like this.
CAUTION
The term CAUTION is used to inform you about situations that
could result in damage to the instruments or other equipment.
Details about these circumstances are given in a box like this.
The advice given in this guide is intended to supplement, not supersede, the normal safety
requirements prevailing in the user’s country.
Proper use
WARNING
Risk of personal injury and material damage
Improper use of the QIAcube Connect
may cause personal
injuries or damage to the instrument. The QIAcube Connect
must only be operated by qualified personnel who have been
appropriately trained. Servicing of the QIAcube Connect must
only be performed by a QIAGEN Field Service specialist.
(W1)
QIAGEN charges for repairs that are required due to incorrect maintenance.

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QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
WARNING
Risk of personal injury and material damage
The QIAcube Connect is too heavy to be lifted by one person.
To avoid personal injury or damage to the instrument, do not lift
the instrument alone.
(W2)
WARNING
Risk of personal injury and material damage
Do not attempt to move the QIAcube Connect during operation.
(W3)
In case of emergency, power OFF the QIAcube Connect at the power switch located in front
of the instrument and unplug the power cord from the power outlet.
CAUTION
Damage to the instrument
Avoid spilling water or chemicals onto the QIAcube Connect.
Damage caused by water or chemical spillage will void your
warranty.
(C1)
In case of emergency, power OFF the QIAcube Connect at the power switch located in front
of the instrument and unplug the power cord from the power outlet.
CAUTION
Damage to the instrument
Only use QIAGEN spin columns and QIAcube Connect specific
consumables with the QIAcube Connect
. Damage caused by
use of other types of spin columns or chemistries will void your
warranty.
(C2)
WARNING
Risk of personal injury and material damage
Do not use damaged rotor adapters. The rotor adapters can
only be used once. High g forces exerted in the centrifuge can
cause damage to used rotor adapters.
(W4)

QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
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CAUTION
Risk of material damage
Empty the tip disposal container prior to use to prevent a tip jam
in the waste drawer. Failure to empty the waste container may
block the robotic arm that could cause run failure or instrument
damage.
(C3)
WARNING
Risk of personal injury and material damage
To prevent plastic crash, load the tubes properly. After a plastic
crash, sharp plastic particles could be inside the centrifuge. Be
careful when handling items inside the centrifuge.
(W5)
CAUTION
Damage to the instrument
Only
use the correct volume of liquids. Exceeding the
recommended volume of liquids may damage the centrifuge
rotor or instrument.
(C4)
WARNING
Risk of fire or explosion
When using ethanol or ethanol-based liquids on the QIAcube
Connect, handle such liquids carefully and in accordance with
the required safety regulations. If liquid has been spilled, wipe
it off and leave the QIAcube Connect
hood open to allow
flammable vapors to disperse.
(W6)
WARNING
Risk of explosion
The QIAcube Connect
is intended for use with reagents and
substances supplied with QIAGEN kits or other than outlined in
respective Information for use. Use of other reagents and
substances may lead to fire or explosion.
(W7)

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QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
If there are hazardous material inside the QIAcube Connect, you are responsible to perform
the appropriate decontamination.
Note: Do not place items on top of the QIAcube Connect hoods.
CAUTION
Damage to the instrument
Do not lean against the touchscreen when it is pulled out.
(C5)
Electrical safety
Note: Disconnect the line power cord from the power outlet before servicing.
WARNING
Electrical hazard
Any interruption of the protective conductor (earth/ground lead)
inside or outside the instrum
ent or disconnection of the
protective conductor terminal is likely to make the instrument
dangerous. Intentional interruption is prohibited.
Lethal voltages inside the instrument
When the instrument is connected to line power, terminals
may be live and opening covers or removing parts is likely to
expose live parts.
(W8)
WARNING
Damage to electronics
Before powering ON the instrument, make sure that the correct
supply voltage is used. Use of incorrect supply voltage may
damage th
e electronics. To check the recommended supply
voltage, refer to the specifications indicated in the type plate of
the instrument.
(W9)

QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
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WARNING
Risk of electric shock
Do not open any panels on the QIAcube Connect.
Risk of personal injury and material damage
Only perform maintenance that is specifically described in this
user manual.
(W10)
To ensure satisfactory and safe operation of the QIAcube Connect, follow these guidelines:
The line power cord must be connected to a line power outlet that has a protective
conductor (earth/ground).
Do not adjust or replace internal parts of the instrument.
Do not operate the instrument with any covers or parts removed.
If liquid has spilled inside the instrument, power OFF the instrument, disconnect it from
the power outlet, and contact QIAGEN Technical Services.
If the instrument becomes electrically unsafe, prevent other personnel from operating it, and
contact QIAGEN Technical Services.
The instrument may be electrically unsafe when:
It or the line power cord appears to be damaged.
It has been stored under unfavorable conditions for a prolonged period.
It has been subjected to severe transport stresses.
Liquids come in contact directly with electrical components of the QIAcube Connect.
Environment
Operating Conditions
WARNING
Explosive atmosphere
The QIAcube Connect is not designed for use in an explosive
atmosphere.
(W11)

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QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
CAUTION
Damage to the instrument
Direct sunlight may bleach parts of the instrument and cause
damage to plastic parts. The QIAcube Connect must be located
out of direct sunlight.
(C6)
Biological safety
Specimens and reagents containing materials from humans should be treated as potentially
infectious. Use safe laboratory procedures as outlined in publications such as Biosafety in
Microbiological and Biomedical Laboratories, HHS (www.cdc.gov/BMBL).
Samples may contain infectious agents. You should be aware of the health hazard presented
by such agents and should use, store, and dispose of such samples according to the required
safety regulations.
WARNING
Samples containing infectious agents
Some samples used with this instrument may contain infectious
agents. Handle such samples with the greatest of care and in
accordance with the required safety regulations.
Always wear safety glasses, 2 pairs of gloves, and a lab coat.
The responsible body (e.g., laboratory manager) must take the
necessary precautions to ensure that the surrounding workplace
is safe, and that the instrument operators are suitably trained
and not exposed to hazardous levels of infectious agents as
defined in the applicable Material Safety Data Sheets (MSDSs)
or OSHA,* ACGIH,†or COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance
with all national, state, and local health and safety regulations
and laws.
(W12)
* OSHA: Occupational Safety and Health Administration (United States of America).
†ACGIH: American Conference of Government Industrial Hygienists (United States of America).
‡COSHH: Control of Substances Hazardous to Health (United Kingdom).

QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
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Chemicals
WARNING
Hazardous chemicals
Some chemicals used with this instrument may be hazardous or
may become hazardous after completion of the protocol run.
Always wear safety glasses, gloves, and a lab coat.
The responsible body (e.g., laboratory manager) must take the
necessary precautions to ensure that the surrounding workplace
is safe and that the inst
rument operators are not exposed to
hazardous levels of toxic substances (chemical or biological) as
defined in the applicable Material Safety Data Sheets (MSDSs)
or OSHA,* ACGIH,†or COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance
with all national, state, and local health and safety regulations
and laws.
(W13)
* OSHA: Occupational Safety and Health Administration (United States of America).
†ACGIH: American Conference of Government Industrial Hygienists (United States of America).
‡COSHH: Control of Substances Hazardous to Health (United Kingdom).
Toxic fumes
If working with volatile solvents or toxic substances, you must provide an efficient laboratory
ventilation system to remove vapors that may be produced.
WARNING
Toxic fumes
Do not use bleach to clean or disinfect the QIAcube Connect.
Bleach in contact with salts from the buffers can produce toxic
fumes.
(W14)
WARNING
Toxic fumes
Do not use bleach to disinfect used labware. Bleach in contact
with salts from the buffers used can produce toxic fumes.
(W15)

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QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
Waste disposal
Used labware, such as sample tubes, QIAGEN spin columns, filter-tips, buffer bottle and
enzyme tubes, or rotor adapters, may contain hazardous chemicals or infectious agents from
the purification process. These hazardous wastes must be collected and disposed of properly
according to local safety regulations.
WARNING
Hazardous chemicals and infectious agents
The waste may contain toxic material and must be disposed of
properly. Refer to your local safety regulations for proper
disposal procedures.
(W16)
Mechanical hazards
The hood of the QIAcube Connect must remain closed during operation of the instrument. Only
open the hood when instructed to do so by the instruction for use.
While loading the worktable, always stand clear of the instrument. Do not lean on the
worktable when the robotic arm of the instrument is moving to reach loading position with its
lid open. Wait until the robotic arm completed its movements before you start to load or
unload.
WARNING
Moving parts
Avoid contact with moving parts during operation of the
QIAcube Connect
. Under no circumstances should you place
your hands under the robotic arm when it is lowering. Do not
attempt to move any tip racks or tubes whilst the instrument is
operating.
(W17)

QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
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WARNING
Moving parts
To avoid contact with moving parts during operation of the
QIAcube Connect
, the instrument must be operated with the
hood closed. If the hood sensor or lock is not functioning
correctly, contact QIAGEN Technical Services.
(W18)
Centrifuge
Make sure that the rotor and buckets are installed correctly. All buckets must be mounted
before starting a protocol run, regardless of the number of samples to be processed. If the
rotor or buckets show signs of mechanical damage or corrosion, do not use the QIAcube
Connect; contact QIAGEN Technical Services.
CAUTION
Damage to the instrument
The QIAcube Connect must not be used if the centrifuge lid is
broken, or if the lid lock is damaged. Make sure that no loose
material is inside the centrifuge during operation.
Make sure that the rotor is installed correctly and that all buckets
are properly mounted, regardless of the number of samples to
be processed. Load the rotor only as instructed by the software.
Only use rotors, buckets, and consumables designed for use
with the QIAcube Connect. Damage caused by use of other
consumables will void your warranty.
We recommend replacing the centrifuge rotor and buckets after
20,000 cycles, which is equivalent to 9 years of usage with two
runs per day for 220 days each year. For more information
contact QIAGEN Technical Services.
(C7)
In case of breakdown, caused by power failure, the centrifuge lid can be manually opened to
remove the samples.

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QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
WARNING
Moving parts
In case of breakdown caused by power failure, remove the
power cord and wait 10 minutes before attempting to
manually open the centrifuge lid.
(W19)
CAUTION
Damage to the instrument
After a power failure, do not move the z-module (robotic arm)
manually in front of the instrument. Damage may occur if the
QIAcube Connect hood is closed and collides with the z-
module.
(C8)
WARNING
Risk of personal injury and material damage
Raise the centrifuge lid carefully. The lid is heavy and may
cause injury if it falls.
(W20)
CAUTION
Risk of overheating
To ensure proper ventilation, maintain a minimum clearance of
10 cm at the sides and rear of the QIAcube Connect.
Slits and openings that ensure the ventilation of the QIAcube
Connect must not be covered.
(C9)
Heat hazard
The QIAcube Connect worktable contains a heated shaker.
WARNING
Hot surface
The shaker can reach temperatures of up to 70°C (158°F).
Avoid touching it when it is hot. Carefully remove the samples
after a run.
(W21)

QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
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Maintenance safety
WARNING/
CAUTION
Risk of personal injury and material damage
Only perform maintenance that is specifically described in this
user manual.
(W22)
WARNING
Risk of explosion
When cleaning the QIAcube Connect with alcohol-based
disinfectant, leave the QIAcube Connect
hood open to allow
flammable vapors to disperse. Only clean the QIAcube Connect
when worktable components have cooled down.
(W23)
WARNING
Risk of fire
Do not allow cleaning fluid or decontamination agents to come
into contact with the electrical parts of the QIAcube Connect.
(W24)
WARNING
Risk of personal injury and material damage
To prevent the rotor nuts from loosening during operation of the
centrifuge, securely tighten the nuts using the rotor key supplied
with QIAcube Connect.
(W25)
WARNING
UV irradiation hazard
A mechanical lock ensures that the hood must be closed for
operation of the UV LED.
If the hood sensor or lock is not
functioning correctly, contact QIAGEN Technical Services.
(W26)
WARNING
Risk of personal injury and material damage
Make sure that lids from spin columns and 1.5 ml
microcentrifuge tubes are in the correct position and pushed all
the way down to the bottom of the slots on the sides of the rotor
adapter. Incorrectly positioned lids can break off during
centrifugation.
(W27)

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QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
WARNING
Risk of personal injury and material damage
Be sure the lid is completely removed from the spin column. Spin
columns with partially removed lids may not be removed
properly from the rotor, causing the protocol run to crash.
(W28)
CAUTION
Damage to the instrument(s)
Do not use bleach, solvents, or reagents containing acids,
alkalis, or abrasives to clean QIAcube Connect.
(C10)
CAUTION
Damage to the instrument(s)
Do not use spray bottles containing alcohol or disinfectant to
clean surfaces of the QIAcube Connect. Spray bottles should
be used only to clean items that have been removed from the
worktables.
(C11)
Radiation safety
WARNING
Risk of personal injury
Do not expose your skin to UV-C light from the UV LED lamp.
(W29)
WARNING
Risk of personal injury
Hazard Level 2 laser light: Do not stare into the light beam when
using handheld barcode scanner.
(W30)

QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
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Translations of Warnings and Cautions
This subsection contains translations of the warnings and cautions used in this guide. Each
warning or caution has a reference number in square brackets at the top right of its box.
Warnings
WARNING
The term WARNING is used to inform you about situations that
could result in personal injury to you or other persons.
Details about these circumstances are given in a box like this.
DE WARNING (WARNUNG)
WARNUNG weist auf Situationen und Umstände hin, die zu einer
Verletzung des Benutzers oder anderer Personen führen können.
Nähere Angaben zu der Art der Gefährdung und der Vermeidung
solcher Situationen werden in einem Text
feld wie diesem neben der
Warnung gemacht.
FR
WARNING (DANGER)
La formule WARNING (DANGER) est utilisée pour avertir des situations
pouvant occasionner des dommages corporels à l’utilisateur ou à
d’autres personnes.
Les détails sur ces circonstances sont donnés dans un encadré semblable
à celui-ci.

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QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
WARNING
Risk of personal injury and material damage
Improper use of the QIAcube Connect
may cause personal
injuries or damage to the instrument. The QIAcube Connect
must only be operated by qualified personnel who have been
appropriately trained. Servicing of the QIAcube Connect must
only be performed by a QIAGEN Field Service specialist.
(W1)
DE
Gefahr von Verletzungen und Sachbeschädigungen
Die unsachgemäße Bedienung des QIAcube Connect
kann zu einer
Verletzung des Benutzers oder zur Beschädigung des Gerätes führen. Die
Bedienung des QIAcube Connect darf nur durch qualifiziertes Personal,
das entsprechend geschult wurde, erfolgen. Die Wartung des QIAcube
Connect darf nur durch Mitarbeiter des QIAGEN Kundendienstes
durchgeführt werden.
FR
Risque de dommages corporels et matériels
L’utilisation non convenable du QIAcube Connect
peut causer des
blessures ou des détériorations de l’instrument. Le QIAcube Connect ne
doit être utilisé que par du personnel quali
fié qui a été formé de façon
appropriée.
Seul un ingénieur du service après-vente QIAGEN est autorisé à effectuer
des travaux d’entretien sur le QIAcube Connect.
WARNING
Risk of personal injury and material damage
The QIAcube Connect is too heavy to be lifted by one person.
To avoid personal injury or damage to the instrument, do not lift
the instrument alone.
(W2)
DE
Gefahr von Verletzungen und Sachbeschädigungen
Der QIAcube Connect
ist zu schwer um von einer Person gehoben zu
werden. Um Verletzunge
n des Benutzers oder eine Beschädigung des
Gerätes zu vermeiden ist davon abzusehen, das Gerät alleine zu heben.
FR
Risque de dommages corporels et matériels
Le QIAcube Connect est trop lourd pour être soulevé par une personne.
Pour éviter des dommages co
rporels ou matériels, ne pas soulever
l’instrument tout seul.

QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
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WARNING
Risk of personal injury and material damage
Do not attempt to move the QIAcube Connect during operation.
(W3)
DE
Gefahr von Verletzungen und Sachbeschädigungen
Den QIAcube Connect während eines Laufes nicht bewegen.
FR
Risque de dommages corporels et matériels
Ne pas essayer de bouger le QIAcube Connect
pendant son
fonctionnement.
WARNING
Risk of personal injury and material damage
Do not use damaged rotor adapters. The rotor a
dapters can
only be used once. High g forces exerted in the centrifuge can
cause damage to used rotor adapters.
(W4)
DE Gefahr von Verletzungen und Sachbeschädigungen
Verwenden Sie keine beschädigten Rotoradapter. Rotoradapter sind nur
für den Einmalgebra
uch konzipiert. Benutzen Sie die Rotoradapter kein
zweites Mal, da sie durch die hohen g-
Kräfte bei der Zentrifugation
beschädigt werden können.
FR Risque de dommages corporels et matériels
N’utilisez pas d’adaptateurs pour rotor abîmés. Les adaptateurs pour
rotor sont à usage unique. L’action importante de la force g exercée à
l’intérieur de la centrifugeuse risque d’abîmer les adaptateurs pour rotor
déjà utilisés.
WARNING
Risk of personal injury and material damage
To prevent plastic crash, load the tubes properly. After a plastic
crash, sharp plastic particles could be inside the centrifuge. Be
careful when handling items inside the centrifuge.
(W5)

20
QIAcube Connect Safety Instructions and Quick Start Guide 04/2019
DE
Gefahr von Verletzungen und Sachbeschädigungen
Um eine Beschädigung von Ku
nststoffteilen zu vermeiden, müssen die
Röhrchen ordnungsgemäß geladen werden. Sollten Kunststoffteile
beschädigt sein, können sich scharfe Kunststoffstücke innerhalb der
Zentrifuge befinden. Bei der Handhabung von Gegenständen innerhalb
der Zentrifuge ist daher Vorsicht angebracht.
FR
Risque de dommages corporels et matériels
Pour éviter tout écrasement du plastique, chargez les tubes correctement.
Suite à l’écrasement du plastique, il se peut que des particules de
plastique pointues se trouvent à l’intér
ieur de la centrifugeuse. Faites
attention lors de la manipulation des éléments à l’intérieur de la
centrifugeuse.
WARNING
Risk of fire or explosion
When using ethanol or ethanol-based liquids on the QIAcube
Connect, handle such liquids carefully and in accordance with
the required safety regulations. If liquid has been spilled, wipe
it off and leave the QIAcube Connect
hood open to allow
flammable vapors to disperse.
(W6)
DE
Brand- oder Explosionsgefahr
Bei der Verwendung von Ethanol oder von Flüssigkeiten auf Ethanolbasis
auf dem QIAcube Connect
müssen diese Flüssigkeiten vorsichtig und in
Übereinstimmung mit den erforderlichen Sicherheitsbestimmungen
gehandhabt werden. Entfernen Sie verschüttete Flüssigkeiten direkt mit
den daf
ür vorgesehenen Materialien. Lassen Sie dabei die Haube des
QIAcube Connect
geöffnet, sodass sich entzündbare Dämpfe
verflüchtigen können.
FR
Risque d’incendie ou d’explosion
En cas d’utilisation d’éthanol ou de liquides à base d’éthanol sur le
QIAcube Connect
, manipulez ces liquides avec prudence et
conformément aux règles de sécurité nécessaires. En cas de déversement
de liquide, essuyez-le et laissez le capot du QIAcube Connect ouvert pour
que les vapeurs inflammables puissent s’évaporer.