Qiagen Hybrid Capture Rotary Shaker 1 User manual

Sample to Insight__
September 2018
Hybrid Capture®System
Rotary Shaker 1 User Manual
6000-2110E (120 V)
6000
-2240E (230 V)
QIAGEN
19300 Germantown Road
Germantown, MD 20874
USA
QIAGEN GmbH
QIAGEN Strasse 1
40724 Hilden
GERMANY
1108562 Rev. 01

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Hybrid Capture System Rotary Shaker 1 User Manual 09/2018
Trademarks: QIAGEN®, Sample to Insight®, digene®, HC2®, Hybrid Capture®(QIAGEN Group).
Registered names, trademarks, etc., used in this document, even when not specifically marked as such, are not to be considered unprotected by law.
© 2018 QIAGEN, all rights reserved.

Hybrid Capture System Rotary Shaker 1 User Manual 09/2018
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Contents
1Introduction ..................................................................................................... 5
1.1 General information................................................................................ 5
1.1.1 Technical assistance........................................................................ 5
1.1.2 Version management....................................................................... 5
1.2 Intended use .......................................................................................... 5
2Safety Information ............................................................................................ 6
2.1 Proper use ............................................................................................. 7
2.2 Electrical safety ...................................................................................... 9
2.3 Biological safety ..................................................................................... 9
2.4 Waste disposal .................................................................................... 11
2.5 Symbols............................................................................................... 12
3Installation..................................................................................................... 14
3.1 Unpacking ...........................................................................................14
3.2 Start up ............................................................................................... 14
4Functional Description..................................................................................... 16
4.1 Operational modes............................................................................... 17
5General Operation .........................................................................................19
5.1 Loading Microplates ............................................................................. 19
5.2 Continuous operation ............................................................................ 20
5.3 Timer-terminated operation .................................................................... 20
5.4 Timer function – Accumulated time.......................................................... 21
5.5 Timer function – Remaining time ............................................................. 21

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5.6 Beeper Preference................................................................................. 22
6Maintenance..................................................................................................23
6.1 Cleaning and decontamination .............................................................. 23
6.2 Regular maintenance............................................................................. 24
6.3 Detaching and replacing the shaker platform ........................................... 25
6.4 Replacing the fuse................................................................................. 26
6.5 Shaking speed verification ..................................................................... 27
6.6 Servicing .............................................................................................28
7Troubleshooting..............................................................................................29
8Technical Data ...............................................................................................33
8.1 Operating conditions ............................................................................ 33
8.2 Transport conditions.............................................................................. 35
8.3 Storage conditions ................................................................................ 35
Appendix A – Waste Electrical and Electronic Equipment (WEEE) .................................. 36
Appendix B – Warranty ............................................................................................ 37
Appendix C — FCC Declaration ................................................................................ 38
Ordering Information ................................................................................................ 40

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1Introduction
The Hybrid Capture System (HCS) Rotary Shaker 1 is an instrument designed toshake
microplates and consists ofa shaker baseand a shaker platform.
Read this user manual prior to operating the HCS Rotary Shaker 1.
1.1 General information
1.1.1 Technical assistance
For technical assistance and more information, please see our Technical Support Center at
www.qiagen.com/TechSupportCenter or contact QIAGEN Technical Services or a local
distributor.
1.1.2 Version management
This document is Hybrid Capture System Rotary Shaker 1 User Manual;see the front cover of
this user manual for document number and revision.
1.2 Intended use
The HCSRotary Shaker 1 is intended for use in conjunction with digene Hybrid Capture 2
(HC2®) DNA tests. The HCS Rotary Shaker 1 is designed for shaking microplates.

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2Safety Information
This manual contains information about warnings and cautions that must be followed by the
user to ensure safe operation of the HCS Rotary Shaker 1 and to maintain the instrument in a
safe condition.
WARNING The term WARNING is used toinform you about situations that could result
in personal injury to you or other persons.
Details about these circumstances are provided to avoid personal injury to
you or other persons.
CAUTION The term CAUTION is used toinform you about situations that could result in
damage to the instrument orother equipment.
Details about these circumstances are provided to avoid damage to the
instrument or other equipment.
Before using the instrument, it is essential to read this manual carefully and to pay particular
attention to any details it contains concerning hazards that may arise from the use of the
instrument.
The details given in this manual are intended to supplement, not supersede, the normal safety
requirements prevailing in the user’s country.

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2.1 Proper use
WARNING/
CAUTION
WARNING/
CAUTION
WARNING/
CAUTION
Risk of personal injury and material damage
Improper use of the HCS Rotary Shaker 1 may cause personal injuries to the
user ordamage to the instrument.
The HCS Rotary Shaker 1 must only be operated by qualified personnel who
have been appropriately trained.
Risk of personal injury and lnstrument damage
Prior to operation, secure the HCS Rotary Shaker 1 to alevel, smooth,
stable surface by firmly pressing down on the four (4) corners of the unit,
creating a strong suction to the work surface (DO NOT place on a bench
mat).Failure to do so will cause excess vibration and could cause personal
injury, Instrument damage, and/or property damage.
Riskof lnstrument damage
Spills should be removed promptly. DO NOT use a cleaning agent or solvent
on the front panel which is abrasive or harmful to plastics, nor onewhich is
flammable. Always ensure the power is disconnected from theunit prior to
any cleaning.
WARNING Risk of personal injury
Always wear shatterproof eye protection.

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Hybrid Capture System Rotary Shaker 1 User Manual 09/2018
WARNING Risk of material damage
WARNING/
CAUTION
WARNING/
CAUTION
Only a QIAGEN Field Service Specialist can service or repair the instrument.
The only exceptions are the maintenance activities listed in the
“Maintenance” section, page 23, of this user manual.
Risk of personal injury and material damage
Do not immerse theHCS Rotary Shaker 1 in water or pour liquids over the
instrument as electrical shock may occur.
Risk of improper operation
Do not use this device in close proximity to sources of strong electromagnetic
radiation (e.g. unshielded intentional RF sources), asthese can interfere with
the proper operation.
Take the following precautions when operating or working near the HCS Rotary Shaker 1:
Always make certain that the shaker platform and any hardware are secure before
operating the HCS Rotary Shaker 1.
Load the HCS Rotary Shaker 1 symmetrically. Avoid unbalanced loads. When shaking a
single microplate, a second empty microplate must be placed diagonally to balance the
load.Likewise, when shaking 3 microplates, an empty microplate must be placed in the
fourth position to balance the load.
Do not use solvents and flammables on or near the HCS Rotary Shaker 1.

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Use the instrument in a dry, clean environment.
Wipe down the base and platform of the HCS Rotary Shaker 1 after each use with a
soft, dry cloth.
Clean up any spills immediately.
Do not allow dust to accumulate on the unit.
If needed, the shaker platform may be removed and cleaned with a cloth dampened in a
dilute detergent solution. See “Maintenance,” page 23, for additional instructions.
Avoid cold starts: Unit is not designed to start after being in a cold room environment.
Bring unit into cold room from a room temperature environment, operate and remove unit
from cold room as soon as operation is complete.
2.2 Electrical safety
Only operate the HCS Rotary Shaker 1 with the power cord provided with the instrument. For
satisfactory and safe operation of the HCS Rotary Shaker 1, it is essential that the line power
cord is connected to true electrical earth (ground).
2.3 Biological safety
WARNING Hazardous substances
The products used with this instrument may contain hazardous substances.
When working with chemicals, always wear a suitable lab coat, disposable
gloves and protective goggles. For more information, please consult the
appropriate safety data sheets (SDSs). These are available online in PDF
format atwww.qiagen.com/safety where you can find, view and print the
SDS for each QIAGEN kit and kit component. For further information see
the instructions for use that comes with the kit.

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Hybrid Capture System Rotary Shaker 1 User Manual 09/2018
WARNING Risk of exposure to hazardous material
Shake hazardous samples only in appropriate containment vessels.
WARNING/
CAUTION
Risk of personal injury and material damage
Consider any laboratory equipment used for research or clinical analysis a
potential biohazard that requires decontamination before reuse.
WARNING Risk of personal injury
Sodium hypochlorite solution is caustic; wear rubber gloves and eye
protection when handling it.
To dispose of the HCS Rotary Shaker 1, follow all national, state and local health and safety
regulation and laws for disposing of laboratory waste. For disposal of Waste Electrical and
Electronic Equipment (WEEE compliance), see “Appendix A – Waste Electrical and Electronic
Equipment (WEEE),” page 36.

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2.4 Waste disposal
Waste may contain certain hazardous chemicals or contagious/biohazardous materials and
must be collected and disposed of properly in accordance with all national, state and local
health and safety regulations and laws.

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2.5 Symbols
The following symbols may be found on the instrument, in this user manual or on labels
associated with the instrument.
Symbol
Location
Description
On the instrument General warning sign
Type plate on the
instrument
CE mark for Europe
Type plate on the
instrument
In vitro diagnostic medical device
Type plate on the
instrument
RoHS mark for China (the restriction of the use
of certain hazardous substances in electrical
and electronic equipment)
Type plate on the
instrument
Waste Electrical and Electronic Equipment
(WEEE)
SN: Type plate on the
instrument
Serial number
Type plate on the
instrument
Manufacturer

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Symbol
Location
Description
Type plate on the
Instrument
RCM mark for Australia
Type plate on the
Instrument
The instrument complies with applicable
standards for electrical safety of laboratory
equipment
UDI Label on the
instrument
Global trade item number
Instrument box label Fragile, handle with care
Instrument box label Consult instructions for use
Front cover of this user
manual
Authorized representative in the European
Community
Front cover of this user
manual
Catalog number

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Hybrid Capture System Rotary Shaker 1 User Manual 09/2018
3Installation
3.1 Unpacking
Before using the HCS Rotary Shaker 1 for the first time, examine the exterior carton and the
equipment itself for damage. In the event of shipping damage, contact your local QIAGEN
representative or QIAGEN Technical Services.
Carefully unpack the instrument and verify the contents of the package, which should contain
the following components of the instrument:
HCS Rotary Shaker 1
1 power cord
If any of these items are missing, contact your local QIAGEN representative or QIAGEN
Technical Services. Save the original packaging until the instrument is operated successfully.
3.2 Start up
Make sure to select the correct voltage rating by checking the type plate on the side of the
instrument. Record the serial number, located on the type plate, for future reference. Turn the
unit upside down, gently rest it on the shaking platform, and inspect the suction cup feet.
Remove any dust or debris from the suction cup feet with 70% Isopropyl alcohol and a lint-free
cloth. Likewise, prepare the surface where the HCS Rotary Shaker 1 will be placed by wiping
it with 70% Isopropyl alcohol and a lint-free cloth.
Place the HCS Rotary Shaker 1 on a level, smooth, stable surface near a grounded electrical
outlet. Allow at least 8 cm (3 inches) clearance on all sides of the instrument for proper
ventilation. Make sure the shaker platform will not touch other objects while in operation.

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Secure the HCS Rotary Shaker 1 to the work surface by firmly pressing down on the four (4)
corners of the unit, creating a strong suction to the work surface (DO NOT place on a bench
mat).
WARNING/
CAUTION
Risk of personal injury and lnstrument damage
Failure to create a strong suction to secure the Shaker will cause excess
vibration, personal injury, Instrument damage, and/or property damage.
At a corner of the instrument, attempt to push the instrument sideways with a medium amount
of force. If the instrument is properly suctioned to the surface, it will not move.
Plug the power cord into a grounded electrical outlet.

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4Functional Description
The HCS Rotary Shaker 1 is a shaker platform attached to a shaker base with 4 screws. The
shaker platform is spring-loaded, and can secure four 96-well microplates. The HCS Rotary
Shaker 1 can hold up to 4 microplates.
The HCS Rotary Shaker 1 can be used in environments from -10 to 60°C, allowing for cold
room use as well as incubated applications.
The HCS Rotary Shaker 1 is constructed of heavy-gauge metal that provides a stable base for
a steady, vibration-free operation.
The following figures show the major external components of the instrument.
All of the operating controls for the HCS Rotary Shaker 1 are located on the front panel. The
following figure shows the front panel and details of the shaker platform.
1
2
4
5
6
7
3
8 9

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Power/Standby button Shaking indicator light
Standby Indicator light Shaking on/off button
Timer up/down arrows Speed display
Timer on/off button Time display
Speed up/down arrows
The power inlet and fuse drawer are on the back of the HCS Rotary Shaker 1.
Power inlet module
Fuse drawer
4.1 Operational modes
The speed of the Shaker 1 is variable, from 100–1200 revolutions per minute (RPM). It
operates in a circular motion with an orbit of 0.3 cm (0.12 inches).
The HCS Rotary Shaker 1 features a timer with an operating time range from 0 to 9,999
minutes in one (1) second increments.
1
2
1
2
1
2
6
7
3 8
9
4
5

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The HCS Rotary Shaker 1 can operate in both Continuous Operation or Timer Terminated
operation modes.

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5General Operation
The HCS Rotary Shaker 1 can be used in either continuous or timer-terminated operation.
Certain load and speed ratios can cause vibration of the instrument. If the instrument vibrates,
adjust the speed and/or load as necessary to eliminate the vibration.
5.1 Loading Microplates
The HCS Rotary Shaker 1 is designed to hold two (2) or four (4) microplates, but must be
loaded symmetrically to avoid unbalanced loads. When shaking a single microplate, a second
empty microplate must be placed diagonally to balance the load. Likewise, when shaking 3
microplates, one empty microplate must be placed in the fourth position to balance the load.
1. Place two (2) microplates diagonally on the platform, or place four (4) microplates on the
platform.
2. Place the corner of the plate under the spring located at each corner of the platform.
3. Slide plate into place.
4. You are ready to use.
2. 3. 4.

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Examples of acceptable microplate loading configurations:
2 Microplates 4 Microplates
5.2 Continuous operation
1. Power ON the instrument by pressing the Power/Standby button.
The standby indicator light turns off, and the speed display and the timer display
illuminate.
2. Press the speed up/down arrows, to set the desired speed.
3. To begin shaking, press the shaking on/off button.
The shaking indicator light will blink rapidly until the desired speed is reached, then it
will remain illuminated. The HCS Rotary Shaker 1 will shake continuously until the
shaking on/off button is pressed.
4. To stop the shaking, press the shaking on/off button.
5.3 Timer-terminated operation
Timer-terminated operation allows for timed shaking procedures.
1. Power ON the instrument by pressing the Power/Standby button.
The standby indicator light turns off, and the speed display and the timer display
illuminate.
2. Press the speed up/down arrows, to set the desired speed.