Qiagen EZ2 Connect User manual

Sample to Insight__
March 2022
EZ2 Connect MDx
Safety Instructions and
Quick-Start Guide
The EZ2 Connect MDx is intended for in vitro diagnostic use.

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
Contents
Introduction................................................................................................................3
Safety Information....................................................................................................... 4
Proper use ....................................................................................................... 4
Electrical safety ................................................................................................ 7
Environment..................................................................................................... 9
Biological safety............................................................................................. 10
Chemicals ..................................................................................................... 12
Toxic fumes .............................................................................................. 12
Waste disposal .............................................................................................. 13
Mechanical hazards ....................................................................................... 13
Heat hazard .................................................................................................. 14
Radiation safety ............................................................................................. 15
Maintenance safety ........................................................................................ 15
Symbols on the EZ2 Connect MDx Instrument............................................................... 18
Installation Procedures............................................................................................... 20
Site requirements............................................................................................ 20
Unpacking and setting up the EZ2 Connect MDx ............................................... 20
To set up the EZ2 Connect MDx.................................................................. 21
Connecting the EZ2 Connect MDx via Wi-Fi................................................. 24
Connecting the EZ2 Connect MDx via LAN cable ......................................... 24
Connecting the EZ2 Connect MDx with LIMS system...................................... 24
Revision History........................................................................................................ 25

EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
3
Introduction
Note: This Quick Start Guide is supplementary to, not a replacement for, the Instrument User
Manual.
The EZ2 Connect MDx system is designed to perform automated isolation and purification of
nucleic acids in molecular diagnostic and/or molecular biology applications.
The EZ2 Connect MDx is intended to be used only in combination with QIAGEN®kits indicated
for use with the EZ2 Connect MDx for the applications described in the kit handbooks.
The EZ2 Connect MDx is intended for use by professional operators, such as technicians and
physicians, trained in molecular biological techniques and the operation of the EZ2 Connect
MDx system.
Important: The
EZ2 Connect MDx User Manual , this Quick-Start Guide, and the available
translations for both documents are electronically available in PDF format. These documents
can be downloaded from the Product Resources tab of the EZ2 Connect MDx webpage
(https://www.qiagen.com/de/products/ez2-connect-mdx/).
The product name, catalog number, and version number on the instructions for use
(user manual and quick start guide) must match the information provided on the product label.
If you require a translation that is not available, please contact QIAGEN Technical Services
immediately.
If you encounter any issues with downloading the instructions for use, QIAGEN will send you
the relevant translation by email, fax, or post. For assistance, please contact QIAGEN
Technical Services at support.qiagen.com or contact one of QIAGEN’s local distributors at
www.qiagen.com.

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
Safety Information
Before using the EZ2 Connect MDx, it is essential that you read this guide carefully and pay
attention to the safety information. The instructions and safety information in this guide must
be followed to ensure safe operation of the instrument and to maintain the instrument in a safe
condition.
WARNING
The term WARNING is used to inform you about situations that
could result in personal injury to you or other persons.
Details about these circumstances are given in a box like this.
CAUTION
The term CAUTION is used to inform you about situations that
could result in damage to the instruments or other equipment.
Details about these circumstances are given in a box like this.
The advice given in this guide is intended to supplement, not supersede, the normal safety
requirements prevailing in the user’s country.
Note: This Quick Start Guide is supplementary to, not a replacement for, the Instrument User
Manual
Proper use
WARNING /
CAUTION
Risk of personal injury and material damage
Improper use of the EZ2 Connect MDx
may cause personal
injuries or damage to the instrument. The EZ2 Connect MDx
must only be operated and maintained by qualified personnel
who has been appropriately trained. Servicing of the EZ2
Connect MDx
must only be performed by QIAGEN Field
Service Specialists.

EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
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WARNING /
CAUTION
Risk of personal injury
The EZ2 Connect MDx is too heavy to be lifted by one person.
To avoid personal injury or damage to the instrument, do not lift
the instrument alone. Use the handle attached to the box to lift
the EZ2 Connect MDx. After the EZ2 Connect MDx has been
unpacked, two people must lift the instrument. Lift the instrument
by placing your hands underneath the bottom of the instrument.
WARNING /
CAUTION
Risk of personal injury and material damage
Do not attempt to move the EZ2 Connect MDx during operation.
CAUTION
Damage to the instrument
Avoid spilling water or chemicals onto the EZ2 Connect MDx.
Instrument damage caused by water or chemical spillage will
void your warranty.
WARNING
Risk of fire or explosion
When using ethanol or ethanol-
based liquids on the
EZ2
Connect MDx, handle such liquids carefully and in
accordance with the required safety regulations. If liquid has
been spilled, wipe it off and leave the EZ2 Connect MDx hood
open to allow flammable vapors to disperse.

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
WARNING
Risk of explosion
The EZ2 Connect MDx is intended for use with reagents and
substances supplied with QIAGEN kits as outlined in the
respective i
nformation for use. Use of other reagents and
substances may lead to fire or explosion.
If hazardous material is spilled on or inside the EZ2 Connect MDx, the user is responsible for
carrying out appropriate decontamination.
Note: Do not place items on top of the EZ2 Connect MDx.
Note: In case of emergency, power OFF the EZ2 Connect MDx and unplug the power cord
from the power outlet.
CAUTION
Damage to the instrument
Ensure that the EZ2 Connect MDx is switched off (power OFF)
and the main plug is removed from power outlet
before you
manually move the mechanical components of the instrument.
CAUTION
Damage to the instrument
Do not lean against the touchscreen.

EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
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Electrical safety
Note: If operation of the instrument is interrupted in any way (e.g., due to interruption of the
power supply or a mechanical error), first switch off the EZ2 Connect MDx then disconnect
the electrical cord from the power supply before attempting troubleshooting.
WARNING
Electrical hazard
Any interruption of the protective conductor (earth/ground lead)
inside or outside the instrument or disconnection of the
protective conductor terminal is likely to make the instrument
dangerous.
Intentional interruption is prohibited.
Lethal voltages inside the instrument
When the instrument is connected to line power, terminals
may be live and opening covers or removing parts is likely to
expose live parts.
WARNING
Damage to electronics
Before powering ON the instrument, make sure that the correct
supply voltage is used.
Use of incorrect supply voltage may damage the electronics.
To check the recommended supply
voltage, refer to the
specifications indicated in the type plate of the instrument.

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
WARNING
Risk of electric shock
Do not open any panels on the EZ2 Connect MDx.
Risk of personal injury and material damage
Only perform maintenance that is specifically described in this
user manual. Any other maintenance or repair may only be
carried out by authorized technical service.
To ensure satisfactory and safe operation of the EZ2 Connect MDx, follow these guidelines:
The line power cord must be connected to a line power outlet that has a protective
conductor (earth/ground).
Place instrument in a location so that the power cord is accessible and can be
connected/disconnected.
Use only the power cord delivered by QIAGEN.
Do not adjust or replace internal parts of the instrument.
Do not operate the instrument with any covers or parts removed.
If liquid has spilled inside the instrument, and has not been wholly contained in the tray,
power OFF the instrument, disconnect it from the power outlet, and contact QIAGEN
Technical Services for advice before attempting troubleshooting.
If the EZ2 Connect MDx becomes electrically unsafe, prevent other personnel from operating
it and contact QIAGEN Technical Services.
The instrument may be electrically unsafe when:
The EZ2 Connect MDx or the line power cord appears to be damaged.
The EZ2 Connect MDx has been stored under unfavorable conditions for a prolonged
period.
The EZ2 Connect MDx has been subjected to severe transport stresses.
Liquids come in contact directly with electrical components of the EZ2 Connect MDx.
The power cord has been exchanged with a non-official power cord.

EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
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WARNING
Electrical hazard
Do not touch the EZ2 Connect MDx with wet hands.
WARNING
Electrical hazard
Never install a fuse different from that specified in the
user manual.
Environment
Operating Conditions
WARNING
Explosive atmosphere
The EZ2 Connect MDx is not designed for use in an explosive
atmosphere.
WARNING
Risk of overheating
To ensure proper ventilation, maintain a minimum clearance of
10 cm at the sides and rear of the EZ2 Connect MDx.
Slits and openings that ensure the ventilation of the instrument
must not be covered.
WARNING
Risk of explosion
The EZ2 Connect MDx is intended for use with reagents and
substances supplied with QIAGEN kits. Use of other reagents
and substances may lead to fire or explosion.

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
CAUTION
Damage to the instrument
Direct sunlight may bleach parts of the instrument, cause
damage to plastic parts, or interfere with the correct function of
the load check. The EZ2 Connect MDx must be located out of
direct sunlight.
CAUTION
Damage to the instrument
Do not use the EZ2 Connect MDx in the vicinity of sources of
strong electromagnetic radiation (e.g., unshielded, deliberately
operated high-
frequency sources or mobile radio devices),
because these can interfere with the proper operation.
Biological safety
Samples and reagents containing materials from humans should be treated as potentially
infectious. Use safe laboratory procedures as outlined in publications such as Biosafety in
Microbiological and Biomedical Laboratories, HHS (https://www.cdc.gov/labs/pdf/CDC-
BiosafetymicrobiologicalBiomedicalLaboratories-2009-P.pdf).
Samples may contain infectious agents. You should be aware of the health hazard presented
by such agents and should use, store, and dispose of such samples according to the required
safety regulations.

EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
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WARNING
Samples containing infectious agents
Samples used with the EZ2 Connect MDx may contain infectious
agents. Handle such samples with the greatest of care and in
accordance with the required safety regulations.
Always wear safety glasses, gloves, and a lab coat.
The responsible persons (e.g., a laboratory manager) must take
the necessary precautions to ensure that the surrounding
workplace is safe and that the instrument operators are suitably
trained and not exposed to hazardous levels of infectious
agents as defined in the applicable Safety Data Sheets (SDSs)
or the OSHA*, ACGIH†, or COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance
with all national, state, and local health and safety regulations
and laws.
* OSHA: Occupational Safety and Health Administration (United States of America).
†ACGIH: American Conference of Government Industrial Hygienists (United States of America).
‡COSHH: Control of Substances Hazardous to Health (United Kingdom).

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
Chemicals
WARNING
Hazardous chemicals
Some chemicals used with the EZ2 Connect MDx
may be
hazardous or may become hazardous after
completion of a
purification.
Always wear safety glasses, gloves, and a lab coat.
The responsible persons (e.g., a laboratory manager) must take
the necessary precautions to ensure that the surrounding
workplace is safe and that the instrument operators ar
e not
exposed to hazardous levels of toxic
substances (chemical or
biological) as defined in the applicable Safety Data Sheets
(SDSs) or the OSHA*, ACGIH†, or COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance
with all national, state, and local health and safety regulations
and laws.
* OSHA: Occupational Safety and Health Administration (United States of America).
†ACGIH: American Conference of Government Industrial Hygienists (United States of America).
‡COSHH: Control of Substances Hazardous to Health (United Kingdom).
Toxic fumes
WARNING
Toxic fumes
Do not use bleach to clean or disinfect the EZ2 Connect MDx.
Bleach in contact with salts from the buffers can produce
toxic fumes.

EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
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WARNING
Toxic fumes
Do not use bleach to disinfect used labware. Bleach in contact
with salts from the buffers can produce toxic fumes.
Note: If you work with volatile solvents, toxic substances, etc., you must provide an efficient
laboratory ventilation system to remove vapors that may be produced.
Waste disposal
Used consumables, such as reagent cartridges and disposable filter-tips, may contain
hazardous chemicals or infectious agents from the purification process. Such waste must be
collected and disposed of properly, according to local safety regulations.
For information on how to dispose of the EZ2 instrument, see Appendix A: Waste Electrical
and Electronic Equipment (WEEE) of the
EZ2 Connect MDx User Manual .
WARNING
Hazardous chemicals and infectious agents
The waste may contain toxic material and must be disposed of
properly. Refer to your local safety regulations for proper
disposal procedures.
Mechanical hazards
The hood of the EZ2 Connect MDx must remain closed during operation of the instrument.
Only open the hood when instructed to do so by the instructions for use or when prompted by
the GUI.
The worktable of the EZ2 Connect MDx moves during operation of the instrument. While
loading the worktable, always stand clear of the instrument. Do not lean on the worktable
when the robotic arm of the instrument is moving to reach loading position with its lid open.
Wait until the robotic arm completed its movements before you start to load or unload.

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
WARNING
Moving parts
To avoid contact with moving parts during the operation of the
EZ2 Connect MDx, the instrument must be operated with the
hood closed.
The hood is locked during operation for safety reasons and a
sensor detects the hood position. If the hood sensor or lock is
not functioning correctly, contact QIAGEN Technical Services.
WARNING
Moving parts
Avoid contact with moving parts during operation of the
EZ2 Connect MDx. Under no circumstances should you place
your hands under the pipetting arm during movement. Do not
attempt to remove any plasticware from the worktable while the
instrument is operating.
Heat hazard
The EZ2 Connect MDx worktable contains a heating system.
WARNING
Hot surface
The heating system can reach temperatures of up to 95°C
(203°F). Avoid touching it when it is hot, in particular, shortly
after a run has been carried out.

EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
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Radiation safety
The EZ2 Connect MDx has a UV LED lamp. The wavelength of the UV light produced by the
UV LED lamp is 270 to 285 nm. This wavelength corresponds to ultraviolet light type C, which
can be used to support decontamination procedures. A mechanical lock ensures that the hood
must be closed for operation of the UV LED. If the hood sensor or lock is not functioning
correctly, contact QIAGEN Technical Services.
WARNING
UV radiation
Avoid looking directly into UV light. Do not expose your skin to
UV light.
WARNING
Risk of personal injury
Hazard Level 2 laser light: Do not stare into the light beam when
using handheld barcode scanner.
Maintenance safety
Perform the maintenance as described in the Maintenance Procedures section of the
EZ2 Connect MDx User Manual . QIAGEN charges for repairs that are required due to
incorrect maintenance.
Operate the EZ2 instrument only as described in the Operating Procedures section of the
EZ2 Connect MDx User Manual . QIAGEN charges for repairs that are required due to
incorrect operation.
WARNING/
CAUTION
Risk of personal injury and material damage
Only perform maintenance that is specifically described in the
EZ2 Connect MDx User Manual .

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
WARNING/
CAUTION
Risk of personal injury and material damage
Improper use of the EZ2 Connect MDx may cause personal
injuries or damage to the instrument.
The EZ2 Connect MDx must only be
operated by suitably
qualified personnel.
Servicing of the EZ2 Connect MDx must only be performed by
QIAGEN Field Service Specialists.
WARNING
Risk of fire
When cleaning the EZ2 Connect MDx with alcohol-based
disinfectant, leave the instrument door open to allow flammable
vapors to disperse.
Only clean the EZ2 Connect MDx with alcohol-based
disinfectant when worktable components have cooled down.
CAUTION
Damage to the instrument
Do not use bleach, solvents, or reagents containing acids,
alkalis, or abrasives to clean the EZ2 Connect MDx.
CAUTION
Damage to the instrument
Do not use spray bottles containing alcohol or disinfectant to
clean surfaces of the EZ2 Connect MDx. Spray bottles should
be used only to clean items that have been removed from the
worktable and if permitted by local laboratory operating
practices.

EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
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CAUTION
Damage to the instrument
After wiping the worktable with paper towels, make sure that
no residual pieces of paper towel remain. Pieces of paper
towel remaining on the worktable could lead to a worktable
collision.
WARNING/
CAUTION
Risk of personal electric shock
Do not open any panels on the EZ2 Connect MDx.
Only perform maintenance as described in the EZ2 Connect
MDx User Manual
.

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
Symbols on the EZ2 Connect MDx Instrument
The following symbols appear on the EZ2 Connect MDx Instrument.
Symbol
Location
Description
Heating system —
inside instrument
Heat hazard — The temperature of the
heating system can reach up to 95ºC
(203°F).
Near the tip rack
Biohazard — The tip rack may be
contaminated with biohazardous material
and must be handled with gloves.
On the back of the
instrument
UV radiation hazard — Avoid looking
directly into UV light. Do not expose your
skin to UV light.
On the handheld
barcode scanner
Hazard Level 2 laser light — Do not stare
into the light beam when using handheld
barcode scanner.
Robotic arm —
inside instrument
Crush hazard — The nozzle unit may crush
your fingers or hand.
Type plate at the
back of the
instrument
CE mark for Europe.
Type plate at the
back of the
instrument
CSA listing mark for Canada and the USA.
Type plate at the
back of the
instrument
RCM (former C-Tick) mark for Australia and
New Zealand

EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
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Symbol
Location
Description
Type plate on the
back of the
instrument
RoHS mark for China (the restriction of the
use of certain hazardous substances in
electrical and electronic equipment).
Type plate at the
back of the
instrument
Waste Electrical and Electronic Equipment
(WEEE) mark for Europe
Type plate at the
back of the
instrument
Legal manufacturer
Type plate on the
back of the
instrument
Unique Device Identifier (UDI) as a 2D bar
code in Data Matrix format
Type plate on the
back of the
instrument
Global Trade Item Number
Type plate on the
back of the
instrument
Serial number
Type plate at the
back of the
instrument
In vitro diagnostic medical device
Type plate on the
back of the
instrument
Consult instructions for use
Type plate at the
back of the
instrument
See warnings and precautions

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EZ2 Connect MDx Safety Instructions and Quick Start Guide 03/2022
Installation Procedures
This section provides you with important information on how to install the EZ2 Connect MDx,
including the required instrument setup and network settings. For detailed unpacking and
installing information, refer to the respective user manual.
The EZ2 Connect MDx is a plug-and-play instrument. Unpacking and installation procedures
are easy to follow, but a person who is familiar with laboratory equipment should oversee the
installation.
Site requirements
The EZ2 Connect MDx must be located out of direct sunlight, away from heat sources, and
away from sources of vibration and electrical interference. Refer to the
EZ2 Connect MDx User
Manual
for the operating conditions (temperature and humidity) and detailed site, power, and
grounding requirements.
Unpacking and setting up the EZ2 Connect MDx
This section describes important actions that must be performed before operating the
EZ2 Connect MDx.
The following items are delivered:
EZ2 Connect MDx instrument
Quick-start guide and safety instructions (this document)
Left and right cartridge racks
Left and right sample/tip racks
Power cord set
USB drive
Silicone grease