Qiagen TissueLyser III User manual

Sample to Insight
May 2023
TissueLyser III Safety Instructions and
Quick-Start Guide

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
Contents
EN 3
Introduction ..................................................................................................... 3
Safety Information ............................................................................................ 3
Installation Procedures..................................................................................... 15
Reference ...................................................................................................... 19
DE 20
Einleitung ...................................................................................................... 20
Sicherheitshinweise......................................................................................... 20
Installationsverfahren ...................................................................................... 36
Referenzen .................................................................................................... 40
FR 41
Introduction ................................................................................................... 41
Informations de sécurité................................................................................... 41
Symboles sur le TissueLyser III .......................................................................... 54
Procédures d’installation.................................................................................. 56
Référence ...................................................................................................... 60
Document Revision History......................................................................................... 61

TissueLyser III Safety Instructions and Quick Start Guide 05/2023
3
EN
Introduction
Thank you for choosing the TissueLyser III system.
Before using the TissueLyser III, it is essential you read this quick-start guide carefully and pay
particular attention to the safety information. The instructions and safety information in this
guide must be followed to ensure safe operation of the TissueLyser III and to maintain the
TissueLyser III in a safe condition. For more detailed information and instructions, visit the
product page of the TissueLyser III on qiagen.com and download the
TissueLyser III
User Manual
.
Safety Information
This instrument is intended for professional use.
The TissueLyser III is to be used only by personnel specifically instructed and trained in biology
laboratory techniques and familiar with this technology.
Before using the TissueLyser III, it is essential that you read this quick-start guide carefully. The
instructions and safety information in the quick-start guide must be followed to ensure safe
operation of the instrument and to maintain the instrument in a safe condition.

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
The following types of safety information appear throughout the TissueLyser III Safety
Instructions and Quick Start Guide
.
WARNING
The term WARNING is used to inform you about situations that could
result in personal injury to you or others.
Details about these circumstances are given in a box like this one.
CAUTION
The term CAUTION is used to inform you about situations that could
result in damage to an instrument or other equipment.
Details about these circumstances are given in a box like this one.
The guidance provided in this guide is intended to supplement, not supersede, the normal
safety requirements prevailing in the user’s country.
Please be aware that you may be required to consult your local regulations for reporting
serious incidents that have occurred in relation to the device to the manufacturer and/or its
authorized representative (only applicable for CE-marked devices with an authorized
representative established in the EU) and the regulatory authority in which the user and/or the
patient is established.

TissueLyser III Safety Instructions and Quick Start Guide 05/2023
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Proper use
WARNING
Risk of personal injury and material damage
Improper use of the TissueLyser III may cause personal injuries or
damage to the instrument. Do not attempt to move the TissueLyser III
during operation.
The TissueLyser III should only be operated by qualified personnel
who have been appropriately trained.
Servicing of the TissueLyser III should only be performed by
QIAGEN®or an authorized agent (service technicians). Do not carry
out any modifications of the instrument.
QIAGEN shall not be held responsible for any injuries or damages
resulting from nonobservance of the instructions and safety
information in this guide.
WARNING
The sound level may be high depending on the type of material, the
number of balls used, the set grinding frequency, and the grinding
time. Excess noise in terms of intensity and duration can lead to
impairments or permanent damage to hearing. Wear hearing
protection.
Excess noise can also lead to acoustic warning signals of other
instruments not being heard. Add visual signals if necessary.
CAUTION
Damage to the instrument
Use of adapters and plasticware other than those described in the
TissueLyser III User Manual may lead to damage to the clamps of the
TissueLyser III.

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
CAUTION
Damage to the instrument
Avoid spilling water or chemicals onto the TissueLyser III. Damage
caused by water or chemical spillage will void your warranty.
CAUTION
Damage to the instrument
Operation of the TissueLyser III with the transport security device
installed, or transport of the TissueLyser III without the transport
security device installed, can lead to damage to the mechanical
components of the instrument.
CAUTION
Damage to the instrument
Operation of the TissueLyser III at a temperature and humidity outside
the range specified may lead to damage to its electronic and
mechanica
l components as well as a reduction in instrument
performance.
WARNING
Risk of personal injury and material damage through falling of the
instrument
Improper handling of the TissueLyser III during transport and
installation can lead to user injury. Particularly when lifting the
instrument above head height, the instrument can fall and lead to
serious injury.
Do not transport the instrument alone. Transport the instrument close
to the floor and avoid lifting above the head.
Install the instrument on a sufficiently large, solid, and stable surface
that is protected against oscillations. Ensure that all instrument feet
are sitting stably and evenly on the surface.

TissueLyser III Safety Instructions and Quick Start Guide 05/2023
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Electrical safety
WARNING
Electrical hazard
Any interruption of the protective conductor (earth/ground lead)
inside or outside the TissueLyser III or disconnection of the protective
conductor terminal is likely to make the TissueLyser III dangerous.
Intentional interruption is prohibited.
Take note of the values for voltage and frequency on the TissueLyser
III type plate and only connect the TissueLyser III to a fitting power
grid.
Only connect the TissueLyser III to the power grid with the enclosed
power cable and to a power outlet with protective conductor
(earth/ground).
Before each usage, check the power cord and the plug for damage.
Never use the instrument with damaged power cord or plug.
Lethal voltages inside the TissueLyser III.
When the TissueLyser III is connected to line power, terminals may
be live and opening the TissueLyser III or removing parts is likely to
expose live parts.
Install the instrument in a way that allows easy access to the power
cord connector and the power switch.
To ensure satisfactory and safe operation of the TissueLyser III, follow the advice below:
The line power cord must be connected to a line power outlet that has a protective
conductor (earth/ground).
Do not adjust or replace internal parts of the TissueLyser III.
Do not operate the TissueLyser III with any covers or parts removed.
If liquid has spilled inside the TissueLyser III, switch off the TissueLyser III, disconnect it
from the power outlet, and contact QIAGEN Technical Services.

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
If the TissueLyser III becomes electrically unsafe, prevent other personnel from operating
it, and contact QIAGEN Technical Services; the TissueLyser III may be electrically unsafe
when:
It or the line power cord appears to be damaged.
It has been stored under unfavorable conditions for a prolonged period.
It has been subjected to severe transport stresses.
If the TissueLyser III is not in use, switch it off at the power switch. If the TissueLyser III will not
be used for a prolonged period of time, unplug the power cord from the power outlet.
Environment
WARNING
Explosive atmosphere
The TissueLyser III is not designed for use in an explosive atmosphere.
WARNING
Risk of explosion
The TissueLyser III is intended for use with reagents and substances
supplied with QIAGEN kits. Use of other reagents and substances
may lead to fire or explosion.
CAUTION
Damage to the instrument
Direct sunlight may bleach parts of the instrument and cause damage
to plastic parts. The TissueLyser III must be located out of direct
sunlight.

TissueLyser III Safety Instructions and Quick Start Guide 05/2023
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Biological safety
Specimens and reagents containing materials from humans or animals should be treated as
potentially infectious. Use safe laboratory procedures as outlined in publications such as
Biosafety in Microbiological and Biomedical Laboratories , CDC (1).
Samples
Samples may contain infectious agents. You should be aware of the health hazard presented
by such agents and should use, store, and dispose of such samples according to the required
safety regulations.
WARNING
Samples containing infectious agents
Some samples used with the TissueLyser III may contain infectious
agents. Handle such samples with the greatest of care and in
accordance with the required safety regulations.
Always wear safety glasses, 2 pairs of gloves, and a lab coat.
The responsible body (e.g., laboratory manager) must take the
necessary precautions to ensure that the surrounding workplace is
safe and that TissueLyser III operators are suitably trained and not
exposed to hazardous levels of infectious agents as defined in the
applicable Safety Data Sheets (SDSs) or OSHA,* ACGIH,†or
COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance with
all national, state, and local health and safety regulations and laws.
* OSHA: Occupational Safety and Health Administration (United States of America).
†ACGIH: American Conference of Government Industrial Hygienists (United States of America).
‡COSHH: Control of Substances Hazardous to Health (United Kingdom).

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
Chemicals
WARNING
Hazardous chemicals
Some chemicals used with the TissueLyser III may be hazardous or
may become hazardous after completion of the sample disruption
process.
Always wear safety glasses, gloves, and a lab coat.
The
responsible body (e.g., laboratory manager) must take the
necessary precautions to ensure that the surrounding workplace is
safe and that TissueLyser III operators are not exposed to hazardous
levels of toxic substances (chemical or biological) as defined in the
applicable Safety Data Sheets (SDSs) or OSHA,* ACGIH,†or
COSHH‡documents.
Venting for fumes and disposal of wastes must be in accordance with
all national, state, and local health and safety regulations and laws.
* OSHA: Occupational Safety and Health Administration (United States of America).
†ACGIH: American Conference of Government Industrial Hygienists (United States of America).
‡COSHH: Control of Substances Hazardous to Health (United Kingdom).

TissueLyser III Safety Instructions and Quick Start Guide 05/2023
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WARNING
Liquid nitrogen
Works with liquid nitrogen pose risks due to oxygen displacement
and cryogenic burns. Ensure proper ventilation of the room and air
monitoring when handling or storing liquid nitrogen. Avoid spills by
always keeping vessels upright and do not touch with bare skin
vessels that are or have been in exposed to liquid nitrogen. Wear
appropriate protective equipment (safety glasses, gloves, lab coat,
and safety shoes).
The low temperature of liquid nitrogen can change the mechanical
properties of materials. Nitrogen-cooled plastic vessels can become
brittle and break when used in the TissueLyser III. Only use liquid
nitrogen with vessels that have been cleared for such use in the
TissueLyser III.
The responsible body (e.g., laboratory manager) must take the
necessary precautions to ensure that the surrounding workplace is
safe and that TissueLyser III operators are not exposed to the dangers
posed by liquid nitrogen as defined in the applicable Safety Data
Sheets (SDSs) or OSHA,* ACGIH,†or COSHH‡documents.
* OSHA: Occupational Safety and Health Administration (United States of America).
†ACGIH: American Conference of Government Industrial Hygienists (United States of America).
‡COSHH: Control of Substances Hazardous to Health (United Kingdom).
Toxic fumes
If working with volatile solvents, toxic substances, etc., you must provide an efficient laboratory
ventilation system to remove vapors that may be produced.
WARNING
Toxic fumes
Do not use bleach to clean or disinfect used labware. Bleach in
contact with salts from the buffers used can produce toxic fumes.

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
Waste disposal
Used labware, such as sample tubes, may contain hazardous chemicals or infectious agents
from the sample disruption process. Such wastes must be collected and disposed of properly
according to local safety regulations. For information on how to dispose of the TissueLyser III,
see Appendix A of the
TissueLyser III User Manual .
Mechanical and thermal hazards
WARNING
Moving parts
Do not leave the TissueLyser III unattended during operation.
To ensure safety, the TissueLyser III will not operate until the hood is closed.
WARNING
Moving parts
Damage or personal injury can occur if the TissueLyser Adapter Set
or Grinding Jar Set is not securely attached.
Ensure that both adapters and grinding jars are of similar weight. Do
not operate the TissueLyser III with only a single adapter or grinding
jar installed.
WARNING
Danger of burns and scalds
Depending on the sample and the disruption parameters, grinding
jars and samples can heat up considerably during the disruption
process. Only touch the grinding jars with protective gloves and wait
for equilibration to room temperature before opening the jars.

TissueLyser III Safety Instructions and Quick Start Guide 05/2023
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Maintenance safety
WARNING
Risk of electric shock
Do not open any panels on the TissueLyser III. Only perform cleaning
with liquids after disconnecting the instrument from the power grid.
Use liquids only on cloth, never free-flowing.
Risk of personal injury and material damage
Only perform maintenance that is specifically described in the
TissueLyser III User Manual .
CAUTION
Damage to the instrument
Do not use bleach, solvents, or reagents containing acids, alkalis, or
abrasives to clean the TissueLyser III.
Avoid spilling water or chemicals onto the TissueLyser III. Damage
caused by water or chemical spillage will void your warranty.
Symbols on the TissueLyser III
Symbol
Location
Description
Type plate on the back of the
instrument
WEEE about the disposal of waste electrical and
electronic equipment for Europe and rest of the
world.
Type plate on the back of the
instrument
Legal manufacturer
Type plate on the back of the
instrument
Date of manufacture

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
Symbol
Location
Description
Type plate on the back of the
instrument
Consult instructions for use.
Type plate on the back of the
instrument
CE mark for Europe
Type plate on the back of the
instrument
UKCA mark for the United Kingdom
Type plate on the back of the
instrument
Mark of the TÜV Rheinland
Type plate on the back of the
instrument
RCM (former C-Tick) for Australia (supplier
identification N17965)
Type plate on the back of the
instrument
RoHS mark for China (the restriction of the use of
certain hazardous substances in electrical and
electronic equipment)
Type plate on the back of the
instrument
Instrument serial number
Type plate on the back of the
instrument
Global trade item number
Type plate on the back of the
instrument
Unique device identifier

TissueLyser III Safety Instructions and Quick Start Guide 05/2023
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Symbol
Location
Description
Type plate on the back of the
instrument
QIAGEN catalogue number
Type plate on the back of the
instrument
Label number
Type plate on the back of the
instrument
See warnings and precautions
On the back of the instrument Wear hearing protection
On the back of the instrument Refer to user manual
Installation Procedures
Unpacking the TissueLyser III
Before unpacking the TissueLyser III, check whether the cardboard container is damaged. In
case of damage, contact the transporter of the container. The TissueLyser III must not be
knocked, shaken, or thrown during transport; otherwise, its electronic and mechanical
components may be damaged.
Make a note of how the TissueLyser III is packed. If returning the instrument to QIAGEN for
repair, it needs to be repacked using the original packaging. Lift the TissueLyser III with two
people, placing both hands below the base of the instrument.

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
After unpacking the TissueLyser III, check that the following items are supplied:
•TissueLyser III
•Power cords for different countries
• TissueLyser III Safety Instructions and Quick-Start Guide
•Tool to remove the transport security device
If anything is missing, contact QIAGEN Technical Services.
Check that the TissueLyser III is not damaged. If anything is damaged, contact QIAGEN
Technical Services. Make sure that the TissueLyser III has equilibrated to ambient temperature
before operating it. The TissueLyser III should be stored dry when not in use.
Retain the original packaging in case you need to return the TissueLyser III to QIAGEN for
repair. Using the original packaging minimizes damage during transportation of the
TissueLyser III and avoids invalidation of your warranty.
Installation requirements
Removal of the transport security devices
To protect the TissueLyser III during transport, transport security devices are located on the
underside of the instrument and around the clamps. It is important to remove the transport
security devices before usage of the TissueLyser III to avoid damage to the instrument. The
required tool is delivered together with the TissueLyser III.
To remove the transport security devices, follow the following steps:
On the underside of the instrument, an arrow marks the location of the transport security
device (Figure 1).

TissueLyser III Safety Instructions and Quick Start Guide 05/2023
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Rotate the TissueLyser III to the side and place it on a soft surface. Ensure that the
instrument is protected against tilting at all times.
Use the tool to remove the transport security device. It is a screw located in a recess at
the center of the baseplate.
Rotate the TissueLyser III back onto its feet and remove the foam block fixing the clamps
(Figure 2).
Carefully store the transport security devices for future transport of the TissueLyser III.
Figure 1. Bottom transport security device. The first transport security device at the underside of the TissueLyser III is
comprised of a screw that has to be removed with the tool delivered with the instrument. Ensure that the transport
security device is removed as depicted in the figure before operating the TissueLyser III.
Location of the transport security device

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
Figure 2 Inner transport security device. The second transport security device comprises of a foam block between the
clamps. Remove the block.

TissueLyser III Safety Instructions and Quick Start Guide 05/2023
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Site requirements
Place the TissueLyser III on a flat, stable surface, and ensure that there is sufficient space around
and under the instrument for ventilation. The TissueLyser III is for indoor use only under the
following conditions:
•Ambient temperature within the range of 5–40°C (41–104°F)
•Up to 2000 m (6500 ft.) above mean sea level
•Maximum relative humidity of 80% for temperatures up to 31°C, linearly decreasing
down to a relative humidity of 50% at 40°C
Power requirements
The power line to the TissueLyser III should be voltage regulated and surge protected. Make
sure that the voltage rating of the TissueLyser III (see the plate on the back of the instrument) is
compatible with the AC voltage available at the installation site.
Grounding requirements
To protect operating personnel, the National Electrical Manufacturers Association recommends
that the TissueLyser III be correctly grounded (earthed). The instrument should be plugged into
an AC power outlet that has a ground (earth) connection.
Reference
1. Meechan PJ, Hatcher B, Potts J. Biosafety in Microbiological and Biomedical Laboratories
(BMBL)
. 6th ed. Centers for Disease Control and Prevention; 2020.
https://www.cdc.gov/labs/BMBL.html

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TissueLyser III Safety Instructions and Quick Start Guide 05/2023
DE
Einleitung
Vielen Dank, dass Sie sich für das TissueLyser III System entschieden haben.
Lesen Sie vor Inbetriebnahme des TissueLyser III diese Kurzanleitung sorgfältig durch und
beachten Sie insbesondere die Sicherheitshinweise. Die Anweisungen und Sicherheitshinweise
in diesem Leitfaden müssen vom Benutzer befolgt werden, um einen sicheren Betrieb des
TissueLyser III zu gewährleisten und den TissueLyser III in einem sicheren Zustand zu halten.
Um detailliertere Informationen und Anweisungen zu erhalten, besuchen Sie die Produktseite
des TissueLyser III unter qiagen.com und laden Sie das
TissueLyser III Benutzerhandbuch
herunter.
Sicherheitshinweise
Dieses Gerät ist für die Verwendung durch professionelle Anwender vorgesehen.
Der TissueLyser III darf nur von Personal verwendet werden, das speziell in biologische
Labortechniken eingewiesen und darin geschult wurde und mit dieser Technologie vertraut ist.
Bevor Sie den TissueLyser III in Betrieb nehmen, lesen Sie unbedingt diese Kurzanleitung
sorgfältig durch und beachten Sie insbesondere die Sicherheitshinweise. Die Anweisungen
und Sicherheitshinweise in der Kurzanleitung müssen vom Anwender befolgt werden, um einen
sicheren Betrieb des Geräts zu gewährleisten und es in einem sicheren Zustand zu halten.
Im Dokument
TissueLyser III Sicherheitsanweisungen und Kurzanleitung werden die folgenden
Kategorien von Sicherheitshinweisen verwendet: