Shimadzu UV-1800 User manual

206-97040A
Feb. 2008
INSTRUCTION MANUAL
System User's Guide
UV-1800
SHIMADZU
SPECTROPHOTOMETER
Read the instruction manual thoroughly before you use the
product. Keep this instruction manual for future reference.

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UV-1800 SERIES i
Introduction
Read this manual thoroughly before using the instrument.
Thank you for purchasing this instrument. This manual describes installation, operation,
hardware validation, cautions for use, and details on accessories and options. Read the
manual thoroughly before using the instrument. Use the instrument in accordance with
the manual's instructions. Keep this manual for future reference.
IMPORTANT
• If the user or usage location changes, be sure this Instruction Manual is always kept together with the
product.
• If this documentation or the warning labels on the instrument become lost or damaged, promptly obtain
replacements from your Shimadzu representative.
• To ensure safe operation, read the Safety Instructions before using the instrument.
• To ensure safe operation, contact your Shimadzu representative if product installation, adjustment, or re-
installation (after the product is moved) is required.
COPYRIGHT • Information in this publication is subject to change without notice and does not
represent a commitment on the part of the vendor.
• Any errors or omissions which may have occurred in this publication despite the
utmost care taken in its production will be corrected as soon as possible, but not
necessarily immediately upon detection.
• All rights are reserved, including those to reproduce this publication or parts thereof in
any form without permission in writing from Shimadzu Corporation.
• Microsoft and Windows are registered trademarks of Microsoft Corporation in the
United States and/or other countries. Other company names and product names
mentioned in this manual are trademarks or registered trademarks of their respective
companies. TM and ® marks are omitted in this manual.
© 2007-2008 Shimadzu Corporation. All rights reserved.

UV-1800 SERIESii
Safety Instructions
• To ensure safe operation of the instrument, read these Safety Instructions carefully before use.
• Observe all of the WARNINGS and CAUTIONS described in this section. They are extremely important for
safety.
In this manual, warnings and cautions are indicated using the following conventions:
The symbol used in this manual is as follows:
Indicates a potentially hazardous situation which, if not avoided, could result in serious
injury or possibly death.
Indicates a potentially hazardous situation which, if not avoided, may result in minor to
moderate injury or equipment damage.
Emphasizes additional information that is provided to ensure the proper use of this
product.
^Indication of location for related information in the instruction manuals
WARNING
CAUTION
NOTE

UV-1800 SERIES iii
Installation Site Precautions
When using flammable and toxic samples, be sure to install ventilation equipment at an
installation site.
• The weight of this instrument is 15 kg. During installation, consider the entire weight
combined with other instruments.
The lab table on which this instrument is installed should be strong enough to support
the total weight of this instrument. It should be level stable, and have depth of at least
600 mm.
Otherwise, the instrument could tip over or fall off the table.
• Avoid installation sites that are exposed to corrosive gases or excessive dust.
These adverse conditions may be detrimental to maintaining instrument performance
and may shorten its service life.
WARNING
CAUTION

UV-1800 SERIESiv
Installation Precautions
To ensure safe operation, contact your Shimadzu representative for installation, adjustment, or re-installation
after moving the instrument to a different site.
• Take measures to prevent the instrument from falling in the event of earthquake or
other disaster.
Strong vibrations could cause the instrument to fall over, resulting in injury.
• The power supply voltages and power consumptions of this instrument are listed below.
The power supply voltage of the instrument is indicated on the label on the side of the
instrument. Connect the instrument only to a power supply of the voltage indicated;
otherwise, fire or electric shock could result. Check that the power supply voltage is
stable and that its current capacity is sufficient to operate all the components of the
system. If not, the instrument will not operate at its rated performance.
• Ground the instrument
Grounding is necessary to prevent electric shock in the event of an accident or
electrical discharge, and important for ensuring stable operation.
• Do not place heavy objects on the power cord, and keep any hot items away.
The cord could be damaged, resulting in fire, electrical shock or malfunction. If the cord
becomes damaged, contact your Shimadzu representative immediately.
• Do not modify the cord in any way. Do not bend it excessively or pull on it.
The cord could be damaged, resulting in fire, electrical shock or malfunction. If the cord
becomes damaged, contact your Shimadzu representative immediately.
WARNING
Power Supply Voltage
(Indicated on the instrument)
Power
Consumption Frequency
AC100 V-120 V and AC220 V-240 V
(100-120 V and 220-240 V ~) 140 VA 50-60 Hz

UV-1800 SERIES v
Operation Precautions
Precautions for Instrument Inspection, Maintenance,
Adjustment and Care.
• Always wear protective gloves when handling any toxic or biologically infectious
samples.
• Do not use flammable sprays (hair sprays, insecticide sprays, etc.) near the instrument.
They could ignite and cause a fire.
• Do not use mobile phones near the instrument.
They could damage data.
• Unplug the instrument before inspection, maintenance, or parts replacement.
Otherwise, electrical shock or short-circuit accidents could occur.
• Never remove the main cover.
This may cause injury or malfunction of the instrument.
The main cover does not need to be removed for routine maintenance, inspection and adjustment.
Have your Shimadzu representative perform any repairs requiring removal of the main cover.
• If the power cord plug gets dusty, remove the plug from the power outlet and wipe away
the dust with a dry cloth.
If dust is allowed to accumulate, fire could result.
• Replacement parts must be of the specifications given in "1.1 UV-1800 Configuration"
and "6.2 Maintenance Parts".
Use of any other parts may result in instrument damage and malfunction.
• If any water gets onto the instrument, wipe it away immediately to prevent rust. Never
use alcohol or thinner solvents for cleaning the instrument.
They could cause discoloration.
• Dispose waste liquid properly and in accordance with the instructions of your
administrative department.
WARNING
CAUTION
WARNING

UV-1800 SERIESvi
Emergency Operation
In an emergency situation, press the " " side of the power switch located on the right side bottom of the UV-
1800 to turn OFF the instrument.
Operation at Power Outage
In case of electrical failure, perform the following operations:
1. Press the " " side of the power switch located on the right side bottom of the UV-1800 to turn OFF the
instrument.
2. After the power comes back on, start up the UV-1800 normally by following "Installation Precautions" and
"Operation Precautions".
Power OFF
(""side is pressed in.)

UV-1800 SERIES vii
Warning Labels
For safety operation, warning labels are affixed where special attention is required.
Should any of these labels peel off or be damaged, obtain replacements from Shimadzu Corporation.
High Temperature
Light source and light source chamber
are very hot. When replacing the light
source, be sure to turn OFF the power
and check that the light source is
completely cooled.
(Right side)

UV-1800 SERIESviii
Product Warranty
Our company provides a warranty on this product, as stated below.
* If there is a document such as a warranty attached to the product, or there is a separate contract agreed upon
that includes warranty conditions, the rules stated in those documents shall be followed.
Warranty periods for products with special specifications and systems are provided separately.
Details
1. Warranty Period: Please consult your Shimadzu representative for information about the extent of
the warranty.
2. Warranty Description: If failure occurs for reasons attributable to our company during the warranty
period, our company will provide repairs or the replacement of parts without
charge (including USB dongles). However, we may not be able to provide identical
products in the case of products such as PCs, and their peripherals and parts,
which have a short lifespan in the market.
3. Warranty Exceptions: The failures caused by the following events are excluded from the warranty, even
if they occur during the warranty period.
1) The product is handled in an improper way.
2) Repairs or modifications are performed by companies or people
other than our company and our designated companies.
3) This product was used in combination with hardware or software
other than those designated by our company.
4) Device failures and damage to data and software, including the
basic software, that are caused by computer viruses.
5) Device failures and damage to data and software, including the
basic software, that are caused by power failures, including power
outages and sudden drops of voltage.
6) Device failures and damage to data and software, including the
basic software, that are caused by powering off the device without
the proper shutdown procedure.
7) Failures caused by reasons other than the device itself.
8) Failures caused by use in harsh environments, such as in high
temperature or humidity, corrosive gas, or vibration.
9) Failures caused by fires and earthquakes or any other act of
providence, contamination by radio active substances and
hazardous substances, or any other force majeure event including
wars, riots, and crimes.
10) Problems occur because the device is transferred or transported
after installation.
11) Expendable items and parts
Note: Recording media such as floppy disks and CD-ROMs are
considered expendables.

UV-1800 SERIES ix
After-Sales Service and Replacement Parts Availability
Disposal of UV-1800
When disposing of the UV-1800, contact your Shimadzu representative.
Otherwise, be sure to dispose of the product separately from general garbage, in compliance with the
applicable laws or regulations in the country or region where it is used.
When disposing of deuterium (D2) lamp
If the deuterium (D2) lamp should be broken or its life is finished, dispose of the lamp separately from
general garbage. When disposing of the deuterium (D2) lamp supplied from Shimadzu Corporation, select a
method which will not adversely influence the environment or human body, or ask a special disposal dealer
for advice or assistance.
The materials of deuterium (D2) lamp are as follows.
• Metals (Tungsten)
• Quartz glass
• Ceramic
• Plastic
To all users of Shimadzu equipment in the European Union:
Equipment marked with this symbol indicates that it was sold on or after 13th August 2005,
which means it should not be disposed of with general household waste. Note that our
equipment is for industrial/professional use only.
Contact Shimadzu service representative when the equipment has reached the end
of its life. They will advise you regarding the equipment take-back.
With your co-operation we are aiming to reduce contamination from waste electronic and electrical equipment
and preserve natural resource through reuse and recycling.
Do not hesitate to ask Shimadzu service representative, if you require further information.
After-Sales Service If any problem occurs with this instrument, inspect it and take appropriate
corrective action as described in the Section "Troubleshooting". If the problem
persists, or symptoms not covered in the Troubleshooting section occur, contact
your Shimadzu representative.
Replacement Parts
Availability
Replacement parts for this instrument will be available for a period of seven (7)
years after the discontinuation of the product. Thereafter, such parts may cease to
available. Note, however, that the availability of parts not manufactured by
Shimadzu shall be determined by the relevant manufacturers.
Disposal Precautions
Action for Environment (WEEE)
WEEE Mark

UV-1800 SERIESx
Regulatory Information
For Europe:
The product complies with the requirements of the EMC Directive 2004/108/EC and Low Voltage Directive
2006/95/EC.
Product Name: UV-Visible Spectrophotometer
Model Name: UV-1800
Manufacturer: SHIMADZU CORPORATION
ANALYTICAL & MEASURING INSTRUMENTS DIVISION
Address: 1, NISHINOKYO-KUWABARACHO,
NAKAGYO-KU, KYOTO, 604-8511, JAPAN
Authorized Representative in EU: Shimadzu Europa GmbH
Address: Albert-Hahn-Strasse 6-10, 47269 Duisburg, F.R. Germany

UV-1800 SERIES xi
CONTENTS
Chapter 1 General
1.1 UV-1800 Configuration ...................................................................................................................... 1-2
1.2 Components ...................................................................................................................................... 1-3
1.2.1 UV-1800 Main Body, Front and Top Views .......................................................................... 1-3
1.2.2 UV-1800 Main Body, Left Side View..................................................................................... 1-4
1.2.3 UV-1800 Main Body, Right Side View .................................................................................. 1-5
1.2.4 Sample Compartment........................................................................................................... 1-6
1.2.5 Keypad.................................................................................................................................. 1-7
1.2.6 Light Source Compartment...................................................................................................1-8
Chapter 2 Installation
2.1 Installation Site .................................................................................................................................. 2-2
2.1.1 Installation Requirements and Preparation........................................................................... 2-2
2.1.2 Installation Space ................................................................................................................. 2-3
2.2 Connecting Power ............................................................................................................................. 2-4
2.2.1 Verifying Power Supply Voltage ........................................................................................... 2-4
2.2.2 Connecting to the Power Outlet............................................................................................ 2-5
2.2.3 Grounding............................................................................................................................. 2-6
2.3 Checking the Light Source Lamp (D2) .............................................................................................. 2-7
2.4 Operation Precautions....................................................................................................................... 2-8
2.5 Turning ON the Power and Initialization ............................................................................................ 2-9
2.5.1 Power ON/OFF ..................................................................................................................... 2-9
2.5.2 Initialization Operation ........................................................................................................ 2-10
2.5.3 Switching System Language .............................................................................................. 2-12
2.6 Instrument Baseline Correction ....................................................................................................... 2-13
2.7 Performance Check after Installation .............................................................................................. 2-15
2.7.1 Parameter Settings ............................................................................................................. 2-15
2.7.2 Performing Validation ......................................................................................................... 2-16
Chapter 3 Maintenance & Inspection
3.1 Inspection and Maintenance.............................................................................................................. 3-2
3.1.1 List of Periodic Inspection & Maintenance Items.................................................................. 3-2
3.2 Inspecting the Sample Compartment ................................................................................................ 3-3
3.3 Checking and Resetting the Lamp Usage Time ................................................................................ 3-4
3.3.1 Checking Procedure ............................................................................................................. 3-4
3.3.2 Resetting Procedure ............................................................................................................. 3-5
3.4 Replacing the Light Source ............................................................................................................... 3-7
3.4.1 Light Source Specifications .................................................................................................. 3-7
3.4.2 Lamp Replacement Procedure ............................................................................................. 3-8
3.5 Cleaning the Exterior ....................................................................................................................... 3-15
Chapter 4 Replacing the Sample Compartment Parts
4.1 Removing/Installing the Cell Holder................................................................................................... 4-2
4.1.1 Removing the Cell Holder..................................................................................................... 4-2
4.1.2 Installing the Cell Holder....................................................................................................... 4-3
4.2 Removing/Installing the Sample Compartment Unit (Standard)........................................................ 4-4
4.2.1 Removing the Sample Compartment Unit ............................................................................ 4-4
4.2.2 Installing the Sample Compartment Unit .............................................................................. 4-5
4.3 Removing/Installing the Sample Compartment Front Cover ............................................................. 4-7
4.3.1 Removing the Sample Compartment Front Cover and Installing the Front Plate................. 4-7
4.3.2 Installing the Sample Compartment Front Cover.................................................................. 4-8

UV-1800 SERIESxii
Chapter 5 Troubleshooting
5.1 Errors during Initialization .................................................................................................................. 5-2
5.2 Problems: Symptoms and Solutions.................................................................................................. 5-4
Chapter 6 Reference
6.1 Specifications..................................................................................................................................... 6-2
6.1.1 Hardware Specifications ....................................................................................................... 6-2
6.1.2 Software Specifications......................................................................................................... 6-3
6.2 Maintenance Parts............................................................................................................................. 6-8
6.2.1 Consumable Parts ................................................................................................................ 6-8
6.2.2 Maintenance Parts ................................................................................................................ 6-8
6.2.3 Repair Parts .......................................................................................................................... 6-8
6.3 Spectrophotometer Basics................................................................................................................. 6-9
6.3.1 What is Light? ....................................................................................................................... 6-9
6.3.2 Ultraviolet/Visible Spectrum ................................................................................................ 6-11
6.3.3 Bouguer-Beer's Law ........................................................................................................... 6-12
6.3.4 Qualitative Analysis and Quantitative Analysis ................................................................... 6-13
6.3.5 Calibration Curve ................................................................................................................ 6-14
6.3.6 Solvent Selection ................................................................................................................ 6-15
6.3.7 Calibration Curve Curvature ...............................................................................................6-16
6.3.8 Spectrophotometer Types................................................................................................... 6-18
6.4 Measurement System...................................................................................................................... 6-22
6.4.1 Optical System.................................................................................................................... 6-22
6.4.2 Electrical System ................................................................................................................ 6-24
6.5 List of Cells ...................................................................................................................................... 6-25
6.6 Cleaning the Cell ............................................................................................................................. 6-28

UV-1800 SERIES 1-1
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1 Installation
Chapter 1 General
CONTENTS
1.1 UV-1800 Configuration ...................................................................................................................... 1-2
1.2 Components ...................................................................................................................................... 1-3

UV-1800 SERIES1-2
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1.1 1.1UV-1800 Configuration
This instrument is shipped with the following items. Upon opening the shipping container, confirm that
all of the listed parts are accounted for in your shipment.
Fig. 1.1 Standard Contents
Table 1.1 Standard Contents
Check Description Part No. Qty.
1Spectrophotometer 206-25201-91 1
2AC Power Cable 071-60816-12 1
3UV-1800 Instruction Manual-System User's
Guide (This manual)
206-97040 1
4UV-1800 Instruction Manual-Operation
Guide
206-97042 1
5UVProve Software (Install CD) 206-21439-91 1
6UVProve Tutorial (Instruction Manual) 206-94459 1
7Shimadzu User Authentication Tool
Instruction Manual
223-10410 1
1
2
5

UV-1800 SERIES 1-3
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1.2 1.2Components
1.2.1 UV-1800 Main Body, Front and Top Views
Fig. 1.2 Front and Top Views of UV-1800
No. Name Description
1LCD This displays the operation menus and measurement results.
2Keypad This is the input component for giving operation commands and numeric
values to the instrument, etc.
^"1.2.5 Keypad"
3Sample Compartment
Cover
Open/close this cover when setting the measured sample.
^"1.2.4 Sample Compartment"
4Sample Compartment
Set Screws
These are screws for fastening the sample compartment unit.
^"4.2 Removing/Installing the Sample Compartment Unit (Standard)"
5LED This lights when the power to the unit is ON.
6Light Source
Compartment Cover
This is the cover of the light source compartment. Open/close this cover
when replacing the light source lamp.
^"3.4 Replacing the Light Source"
3
5
4
6
2
1

1.2 Components
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1-4
1.2.2 UV-1800 Main Body, Left Side View
Fig. 1.3 Left Side View of UV-1800
No. Name Description
1USB Connector You can use these connecters for any of printer, or USB memory stick.
You can also connect special accessories described below. In such
cases, however, it is necessary to purchase separately and connect the
designated USB conversion adaptor to each accessory.
• 6-cell electronic temperature control cell positioner CPS-240A/B
ÖUSB adaptor for CPS (P/N 206-25234-91)
• Auto sample changer ASC-5
ÖUSB adaptor for ASC (P/N 206-25235-91)
For installation and connection procedures, refer to the instruction
manual of each accessory.
2USB Connector (for PC) This is the connector for connecting PC during external control
operation.
^Operation Guide, "17.1 Connecting to a PC"
3Optional Unit Connector This is the connector to connect the "Sipper 160" or "Syringe sipper"
(special accessories).
For installation and connection procedures, refer to the instruction
manual of each accessory.
4AC Power Connector Connect the enclosed AC power cable to the supply power from an AC
electrical outlet.
^"2.2 Connecting Power"
AC IN
SIPPER
100-120, 220-240V~
50-60H
Z 140VA
AC IN
SIPPER
100-120, 220-240V~
50-60H
Z 140VA
2
3




1




4

1.2 Components
UV-1800 SERIES 1-5
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1.2.3 UV-1800 Main Body, Right Side View
Fig. 1.4 Right Side View of UV-1800
No. Name Description
1USB Connector Plug in the USB memory to this connector.
2Power Switch Use this switch to turn ON/OFF the instrument. Press the "I" side on the
switch to turn ON; press the "O" side to turn OFF.
1
2

1.2 Components
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1-6
1.2.4 Sample Compartment
Fig. 1.5 Sample Compartment
No. Name Description
1Cell Holder The cell holder for the rectangular 10 mm light path cells has one sample
cell holder and one reference cell holder.
^"6.5 List of Cells"
2Cell Holder Set Screws The cell holder can be easily removed by loosening the cell holder set
screws.
^"4.1 Removing/Installing the Cell Holder"
3Sample Compartment
Front Cover
When using a flow cell, etc., holes are needed to pass tubing, etc.
through. Therefore, this "Sample Compartment" can be removed and
exchanged with different types of front panels.
^"4.3 Removing/Installing the Sample Compartment Front Cover"
4Multi-cell Holder Drive
Connector
This is the connector for connecting the control cables for multi-cell
sample compartment and 8/16-cell micro multi cell holder (MMC-1600)
(special accessories).
4
1
2
3
Reference side
cell holder
Measurement
side cell holder