Sigma 4-5KL User manual

Translation of the original operating manual
sb
06/2020
Rev. 1.2 of 28/04/2022
Sigma 4-5KL;Refrigerated centrifuge;Sigma 4-5KL IVD; ;
Refrigerated centrifuge
part no. 0703602
Sigma 4-5KL
Sigma 4-5KL IVD
from serial no. 180163
Refrigerated centrifuge
Operating Manual
Please retain for later use!

Refrigerated centrifuge Sigma 4-5KL
Refrigerated centrifuge Sigma 4-5KL IVD
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Version 06/2020, Rev. 1.2 of 28/04/2022 • sb
Translation of the original operating manual, part no. 0703602

Refrigerated centrifuge Sigma 4-5KL
Refrigerated centrifuge Sigma 4-5KL IVD
Version 06/2020, Rev. 1.2 of 28/04/2022 • sb
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Translation of the original operating manual, part no. 0703602
© Copyright by
Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode am Harz
Germany
Tel.: [phone] / 5007-0
Fax: [phone] / 5007-12
Web: www.sigma-zentrifugen.de
E-mail: [e-mail]

Refrigerated centrifuge Sigma 4-5KL
Refrigerated centrifuge Sigma 4-5KL IVD
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Version 06/2020, Rev. 1.2 of 28/04/2022 • sb
Translation of the original operating manual, part no. 0703602

Refrigerated centrifuge Sigma 4-5KL
Refrigerated centrifuge Sigma 4-5KL IVD
Table of contents
Version 06/2020, Rev. 1.2 of 28/04/2022 • sb
5 / 84
Translation of the original operating manual, part no. 0703602
1General information.............................................................................................................9
1.1 Importance of the operating manual..................................................................................9
1.2 Intended use.....................................................................................................................9
1.3 Warranty and liability.........................................................................................................9
1.4 Copyright........................................................................................................................10
1.5 Standards and regulations..............................................................................................10
1.6 Scope of supply..............................................................................................................10
2Layout and mode of operation..........................................................................................11
2.1 Layout of the centrifuge ..................................................................................................11
2.1.1 Functional and operating elements............................................................................11
2.1.2 Name plate................................................................................................................13
2.2 Mode of operation...........................................................................................................14
2.2.1 Centrifugation principle..............................................................................................14
2.2.2 Area of application.....................................................................................................14
2.2.2.1 Speed, radius, and relative centrifugal force ......................................................15
2.2.2.2 Density...............................................................................................................15
3Safety..................................................................................................................................16
3.1 Marking of the unit..........................................................................................................16
3.2 Explanation of the symbols and notes.............................................................................17
3.3 Responsibility of the operator..........................................................................................18
3.4 Requirements concerning the personnel.........................................................................19
3.5 Informal safety instructions .............................................................................................20
3.6 Safety instructions ..........................................................................................................21
3.6.1 Electrical safety .........................................................................................................21
3.6.2 Mechanical safety......................................................................................................21
3.6.3 Fire prevention...........................................................................................................22
3.6.4 Chemical and biological safety ..................................................................................22
3.6.5 Safety instructions for centrifugation..........................................................................23
3.6.6 Resistance of plastics................................................................................................23
3.6.7 Service life (for centrifuges according to Machinery Directive)...................................24
3.6.8 Service life (for centrifuges according to the IVD regulation)......................................25
3.6.9 Safety of rotors and accessories................................................................................26
3.6.9.1 Marking..............................................................................................................26
3.7 Safety devices ................................................................................................................27
3.7.1 Lid lock device...........................................................................................................27
3.7.2 Standstill monitoring system......................................................................................27
3.7.3 System check............................................................................................................27
3.7.4 Earth conductor check...............................................................................................27
3.7.5 Imbalance monitoring system....................................................................................27
3.7.6 Temperature monitoring system ................................................................................27
3.7.7 Rotor monitoring system............................................................................................27
3.8 Measures in the event of hazards and accidents ............................................................28
3.9 Remaining hazards.........................................................................................................28

Refrigerated centrifuge Sigma 4-5KL
Refrigerated centrifuge Sigma 4-5KL IVD
Table of contents
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4Storage and transport ....................................................................................................... 29
4.1 Dimensions and weight...................................................................................................29
4.2 Storage conditions..........................................................................................................29
4.3 Notes on transport..........................................................................................................29
4.4 Packaging.......................................................................................................................30
4.5 Transport safety device...................................................................................................30
5Set-up and connection ...................................................................................................... 31
5.1 Installation site................................................................................................................31
5.2 Power supply..................................................................................................................31
5.2.1 Type of connection ....................................................................................................31
5.2.2 Customer-provided fuses...........................................................................................32
6Using the centrifuge.......................................................................................................... 33
6.1 Initial start-up..................................................................................................................33
6.2 Switching the centrifuge on.............................................................................................33
6.2.1 Opening and closing the lid........................................................................................33
6.2.2 Installation of rotors and accessories.........................................................................34
6.2.2.1 Installation of a standard rotor............................................................................34
6.2.2.2 Installation of accessories..................................................................................35
6.2.2.3 Adapters ............................................................................................................36
6.2.2.4 Vessels..............................................................................................................36
6.3 Control system "Spincontrol L"........................................................................................37
6.3.1 User interface............................................................................................................37
6.3.2 Manual mode.............................................................................................................38
6.3.2.1 Starting a centrifugation run...............................................................................38
6.3.2.2 Interrupting a centrifugation run .........................................................................38
6.3.2.3 Interrupting a deceleration process....................................................................38
6.3.2.4 Selection, display, and modification of data........................................................38
6.3.2.5 Speed / relative centrifugal force (RCF) .............................................................39
6.3.2.6 Runtime .............................................................................................................39
6.3.2.7 Temperature......................................................................................................41
6.3.2.8 Rotor selection...................................................................................................43
6.3.2.9 Acceleration and deceleration curves.................................................................44
6.3.2.10 Automatic lid opening function ("AutoOpen")......................................................44
6.3.2.11 Sound signal ("Buzzer").....................................................................................45
6.3.2.12 Program lock ("ProgLock").................................................................................45
6.3.2.13 Cycle display ("Cycles").....................................................................................46
6.3.2.14 Input lock ...........................................................................................................47
6.3.2.15 Eco mode...........................................................................................................47
6.3.3 Program mode...........................................................................................................49
6.3.3.1 Saving a program...............................................................................................49
6.3.3.2 Loading a program.............................................................................................50
6.4 Switching the centrifuge off.............................................................................................50

Refrigerated centrifuge Sigma 4-5KL
Refrigerated centrifuge Sigma 4-5KL IVD
Table of contents
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Translation of the original operating manual, part no. 0703602
7Malfunctions and error correction....................................................................................51
7.1 General malfunctions......................................................................................................51
7.1.1 Emergency lid release...............................................................................................52
7.2 Table of error codes........................................................................................................53
7.3 Service contact...............................................................................................................54
8Maintenance and service................................................................................................... 55
8.1 Maintenance...................................................................................................................55
8.1.1 Centrifuge..................................................................................................................55
8.1.1.1 Condenser (only refrigerated centrifuges with an air-cooled refrigeration system)
..........................................................................................................................56
8.1.2 Accessories...............................................................................................................56
8.1.2.1 Plastic accessories ............................................................................................57
8.1.3 Rotors, buckets and carriers......................................................................................57
8.1.4 Load bearing bolts.....................................................................................................58
8.1.5 Glass breakage .........................................................................................................59
8.2 Sterilisation and disinfection of the rotor chamber and accessories ................................59
8.2.1 Autoclaving................................................................................................................60
8.3 Service ...........................................................................................................................61
8.4 Return of defective centrifuges or parts ..........................................................................62
9Disposal.............................................................................................................................. 64
9.1 Disposal of the centrifuge ...............................................................................................64
9.2 Disposal of the packaging...............................................................................................64
10 Technical data....................................................................................................................65
10.1 Ambient conditions .........................................................................................................66
10.2 Technical documentation................................................................................................66
11 Appendix............................................................................................................................ 67
11.1 Range of accessories .....................................................................................................67
11.1.1 Rotor radii..................................................................................................................67
11.2 Speed-gravitational-field-diagram ...................................................................................68
11.3 Acceleration and deceleration curves .............................................................................69
11.4 Table of the service life of rotors and accessories...........................................................70
11.5 Resistance data..............................................................................................................71
11.6 EC declaration of conformity...........................................................................................75
11.7 Declaration of conformity –China RoHS 2......................................................................79
12 Index................................................................................................................................... 81

Refrigerated centrifuge Sigma 4-5KL
Refrigerated centrifuge Sigma 4-5KL IVD
Table of contents
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Version 06/2020, Rev. 1.2 of 28/04/2022 • sb
Translation of the original operating manual, part no. 0703602

Refrigerated centrifuge Sigma 4-5KL
Refrigerated centrifuge Sigma 4-5KL IVD
1 General information
Version 06/2020, Rev. 1.2 of 28/04/2022 • sb
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Translation of the original operating manual, part no. 0703602
1 General information
1.1 Importance of the operating manual
A fundamental requirement for the safe and trouble-free operation of the
centrifuge is to be familiar with the fundamental safety instructions and all
possible hazards.
The operating manual includes important information concerning the safe
operation of the centrifuge.
This operating manual and, in particular, the notes on safety and hazards
must be observed by all persons operating the centrifuge.
In addition, the local rules and regulations for the prevention of accidents
must be complied with.
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1.2 Intended use
The laboratory centrifuge is suitable for the separation of constituents of
different densities in mixtures with a maximum density of 1.2 g/cm3.
The laboratory centrifuge that is marked with IVD is intended for human
biological samples, including donated blood and tissue, in conjunction with
diagnostic in-vitro applications. This means that it is a medical product in
accordance with Regulation (EU) 2017/746 on in vitro diagnostica.
Only trained, specialised personnel are authorised to use the centrifuge in
closed laboratories.
The intended use also includes:
•observation of all of the notes and instructions that are included in the
operating manual and
•compliance with the inspection and maintenance instructions.
Sigma Laborzentrifugen GmbH cannot be held liable for:
•damage resulting from the improper use of the centrifuge not in line with
its intended purpose,
•faulty results that are due to the incorrect or faulty procedures of the
user.
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1.3 Warranty and liability
The warranty and liability are subject to our "General Conditions" that were
distributed to the operator upon the conclusion of the contract.
Warranty and liability claims are excluded if they are due to:
•improper use.
•non-compliance with the safety instructions and hazard warnings in the
operating manual.
•improper installation, start-up, operation, or maintenance of the
centrifuge.

Refrigerated centrifuge Sigma 4-5KL
Refrigerated centrifuge Sigma 4-5KL IVD
1 General information
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1.4 Copyright The copyright concerning the operating manual remains with
Sigma Laborzentrifugen GmbH.
The operating manual is solely intended for the operator and their
personnel. It includes instructions and information that must not be
•duplicated,
•distributed, or
•communicated in any other way.
Non-compliance may be prosecuted under criminal law.
→
1.5 Standards and regulations
These operating instructions have been created in accordance with the
relevant European standards and regulations (see chapter 11.6 - "EC
declaration of conformity").
1.6 Scope of supply
The centrifuge comprises:
•1 power cord with an IEC C13 connector
•1 rotor wrench, size 13
Part no. 930 102
•1 hexagon socket wrench, size 5
(emergency lid release)
Part no. 930 051
•1 hexagon socket wrench, size 4
(transport safety device)
Part no. 930 020
•1 tube (30 g) heavy-duty grease for load-
bearing bolts
Part no. 71 401
Documentation
Operating manual incl. EC declaration of conformity
(see chapter 11.6 - "EC declaration of conformity")
Accessories
According to your order, our order confirmation, and your delivery note.