Star Lab ErgoOne E User manual

STARLAB International GmbH
Neuer Höltigbaum 38
22143 Hamburg
Germany
T: [phone]
F: [phone]
[e-mail]
www.starlabgroup.com
STARLAB GmbH
Neuer Höltigbaum 38
22143 Hamburg
Germany
T: [phone]
F: [phone]
[e-mail]
www.starlab.de
STARLAB (UK), Ltd
5 Tanners Drive
Milton Keynes MK14 5BU
United Kingdom
T: [phone]
F: [phone]
[e-mail]
www.starlab.co.uk
STARLAB FRANCE
30 Rue Jean Rostand
91400 Orsay
France
T: [phone]
F: [phone]
[e-mail]
www.starlab-france.com
STARLAB S.r.l.
Via Pinturicchio 1
20133 Milano
Italy
T: [phone]
F: [phone]
[e-mail]
www.starlabitalia.it
www.starlabgroup.com
User Manual · Bedienungsanleitung · Mode d‘emploi
Istruzioni per l‘uso · Instrucciones de manejo
  
9974 51 • Printed in Germany • 21/0115/1
STARLAB_ErgoOne E MC_7 Rücken_2014 12 09_v2.indd 1 09.12.2014 13:51:12

EC Declaration of Conformity
STARLAB certifies that all variants of
ErgoOne®E
including charging adapter
has been manufactured and inspected in accordance
with the following European standards:
2004/108/EC: EN 61326-1:2006
2006/95/EC: EN 61010-1:2010 - EN 60950-1:2006+A11:2009
As an ISO 9001 and ISO 13485 certified company,
our working procedures and processes are checked
and audited regularly
and the quality of our products is continuously monitored.
STARLAB International GmbH · Neuer Höltigbaum 38 · 22143 Hamburg
www.starlabgroup.com
© December 2014. All rights reserved, including graphics and images. Every effort has been made to ensure that
the content of this publication is correct. STARLAB can not take responsibility for any errors or omissions.
STARLAB_ErgoOne E MC_7 Rücken_2014 12 09_v2.indd 2 09.12.2014 13:51:12

English
TABLE OF CONTENTS
Page
Safety Instructions 4
Functions and Limitations of Use 5
Operating Exclusions 5
Disposal 5
Operating Elements 6
The First Steps 7
Setting the Volume 8
Setting the Aspiration and Discharging Speed 9
Correct Pipetting 10
The Pipetting Programs 11
PIP mode 12
PIPmix mode 14
revPIP mode 16
Electrophoresis (GEL) mode 18
DISP mode 20
Checking the Volume 22
Accuracy Table 23
Easy Calibration (adjustment) 24
Autoclaving 26
Reference Run (rEF) 26
Servicing and Cleaning 27
Charging and Replacing the Battery 32
Battery Regeneration Function 33
Troubleshooting 34
Ordering Information · Accessories · Spare Parts 35
Repairs and Warranty Information 36
Table of Contents
ErgoOne E -8/-12 · User Manual · 3

Please read the following carefully!
This instrument may sometimes be used with hazardous materials, operations, and equipment.
It is beyond the scope of this manual to address all of the potential safety problems associated with its use
in such applications. It is the responsibility of the user of this pipette to consult and establish appropriate
safety and health practices and determine the applicability of regulatory limitations prior to use.
SAFETY INSTRUCTIONS
1. Every user must read and understand this
operating manual prior to using the instrument
and observe these instructions during use.
2. Follow general instructions for hazard preven-
tion and safety instructions; e.g., wear protec-
tive clothing, eye protection and gloves.
When working with infectious or other hazard-
ous samples, all appropriate regulations and
precautions must be followed.
3. Observe all specifications provided by reagent
manufacturers.
4. Never use the instrument in an atmosphere
with a danger of explosion. Highly flammable
liquids must not be pipetted.
5. Only use the instrument for pipetting liquids
that conform to the specifications defined in
the operating exclusions and limitations
(see page 5). If in doubt, contact the manufac-
turer or supplier.
6. Always use the instrument in such a way that
neither the user nor any other person is
endangered. Avoid splashes and only use suit-
able vessel.
7. Avoid touching the tip orifices when working
with hazardous samples.
8. Never use force on the instrument!
9. Only use original spare parts. Do not attempt
to make any technical alterations. Do not
dismantle the instrument any further than is
described in the operating manual!
10. Before use check the instrument for visible
damages. If there is a sign of a potential
malfunction (e.g., piston difficult to move,
leakage), immediately stop pipetting. Consult
the 'Troubleshooting' section of this manual
(see page 34), and contact the manufacturer
if needed.
11. The original battery must not be replaced with
non rechargeable batteries or rechargeable
batteries of other manufacturers.
12. To charge the NiMH battery pack, use only the
original AC adapter.
13. The AC adapter has to be protected against
moisture and must be used only for this instru-
ment.
14. Dispose of batteries only when discharged and
according to applicable regulations.
Improper use of the instrument or the batteries (short circuit, mechanical damage, overheating, incorrect
AC adapter, etc.) can lead to battery explosion.
WARNING!
!
4· ErgoOne E -8/-12 · User Manual

English
FUNCTIONS AND LIMITATIONS OF USE
The ErgoOne®E -8/-12 is a microprocessor-con-
trolled, battery-operated piston-stroke multichan-
nel pipette which uses the air-displacement prin-
ciple for the pipetting of aqueous solutions with an
average density and viscosity.
When the instrument is used properly, the sample
only comes into contact with the tips and not with
the ErgoOne®E -8/-12.
DISPOSAL
The adjoining symbol means that storage batteries and electronic devices must be disposed of separately
from household waste (mixed municipal waste) at the end of their service life.
- According to the Directive 2002/96/EC of the European Parliament and of the Council on Waste Electri-
cal and Electronic Equipment (WEEE) of 27 January 2003, electronic equipment requires disposal accord-
ing to the relevant national disposal regulations.
- Batteries contain substances that can have harmful effects on the environment and hu-
man health. Therefore according to the Directive 2006/66/EC of the European Parlia-
ment and the Council on Waste Batteries of 6 September 2006 batteries require disposal
according to the relevant national disposal regulations. Dispose of batteries only when
completely discharged.
Do not short-circuit the battery to discharge it!
WARNING!
The ErgoOne®E is intended for the pipetting of
liquids within the following limitations:
– Temperature of both the instrument and solution
should be between 15 °C to 40 °C (59 °F to
104 °F) for the instrument and the reagent. Con-
sult the manufacturer for use in temperatures
outside of this range.
– Vapor pressure up to 500 mbar
– Viscosity: 260 mPa s (260 cps)
Battery
Nickel-metal hydride battery with 3 cylindrical
individual cells with size AAA,
3.6 V, 700 mAh
AC adapter
Output voltage 6.5 V DC, 200 mA
Limitations of use
Operating exclusions Battery and AC adapter specifications
Viscous and highly adhesive liquids may impair
volumetric accuracy. Volumetric accuracy may also
be impaired when pipetting liquids that differ from
ambient temperature by more than ± 5 °C / 41 °F.
Operating Limitations
The user has to ensure the compatibility of the
instrument with the intended application.
Never use the instrument for pipetting liquids
that react adversely with polypropylene (PP: shaft
and tips), polycarbonate/polybutyleneterephthalate
(PC/PBT: casing). Avoid reactive vapors due to the
danger of corrosion.
The handle is not autoclavable.
Functions and Limitations of Use
ErgoOne E -8/-12 · User Manual · 5

Display
Program keys
Tip ejection key
Handgrip
Manifold
Nose cone
Pipetting key
Charging jack
OPERATING ELEMENTS
Menu
selection
key
Entry confir-
mation/
Power 'On'*
Pipetting key
UP key (+)
DOWN key (-)
The ErgoOne®E -8/-12 is a microprocessor-controlled, battery-operated,
piston-stroke multichannel pipette, which has been optimized for ergonomic
operation and ease of use.
Battery compartment
Finger rest
*) The instrument is activated by pressing the ENTER key.
Then press the pipetting key, and the instrument is ready
for pipetting.
The ErgoOne®E -8/-12 shuts off 10 min after the last
instrument operation (Auto-Power-Off).
Key Functions
The ErgoOne®E -8/-12
rests easily and ergonomi-
cally in your hand. The finger
rest is height adjustable with a
screw, which allows fine-tuning
of the hand position for effortless
operation of the function buttons.
6· ErgoOne E -8/-12 · User Manual

1x
C
B
A
English
c) Replace the battery compartment.
1. Insert the
battery
Is everything in the package?
Confirm that your package includes: ErgoOne®E -8 or -12 pipette, battery,
power supply unit with battery charging cable, an AC adapter for UK, 1
instrument stand, silicone oil, operating manual and 1 set of V-rings made of
FKM.
a) Open the cover of the battery compartment.
b) Insure that the plug for the battery is firmly
connected to the pipette. Insert the battery.
2. Activate the
instrument
The ErgoOne®E -8/-12 au-
tomatically requests a refer-
ence run directly after the
battery is inserted. After the
pipetting key is pressed, the
reference run is carried out
and the instrument is now
ready for pipetting.
Battery charge
indicator
Up arrow,
indicating
aspiration
Pipetting mode
Volume setting
The display shows the
standard factory setting
(pipetting mode/PIP); and
the nominal volume (for
example, 100.0 µl). Default
aspiration and discharging
speeds are at maximum.
The adjustment of volume
and speed is described on
the following pages.
THE FIRST STEPS
Initializing the ErgoOne®E -8/-12
The First Steps
ErgoOne E -8/-12 · User Manual · 7

1x
+
–
Press the UP key (+) to
increase the volume. Press-
ing and holding the key
down accelerates the rate
of change. 'VOL' continues
to blink.
Press the ENTER key.
The display now shows the
new volume setting, in this
case, 102.8 µl in the PIP
mode.
2. Change the volume
3. Confirm volume
setting
The volume for the ErgoOne®E -8/-12 is set at the factory to the nominal volume of the
instrument and can be changed quickly and easily.
Press the DOWN key (-) to
reduce the volume. Pressing
and holding the key down
accellerates the rate of
change. 'VOL' continues to
blink.
Reduce volume
Press either the UP or DOWN
key to activate volume selec-
tion. 'VOL' blinks.
1. Activate volume
setting
Increase volume
SETTING THE VOLUME
What to do How to do it Keys to press Display readout
By pressing the MENU key any procedure can be cancelled! The display then moves to the next setting or
back to the initial display (depending on actual selection.)
IMPORTANT:
8· ErgoOne E -8/-12 · User Manual

1x
1x
2x
1x
+
–
+
–
English
Press the MENU key once
to bring up the aspiration
speed menu. 'Speed' blinks.
Press either the UP or DOWN
key to select the desired
speed (in this case, level 5).
'Speed' continues to blink.
Press the ENTER key. The
display returns to the start
position for the current
pipetting mode (in this case,
the standard PIP mode).
1. Bring up the menu
2. Change the
aspiration speed
3. Confirm speed
level
The aspiration and discharging speeds can be individually adjusted. When the menu is called up, the last
speed setting is shown. Five speed levels are available.
Press the MENU key twice
to bring up the discharging
speed menu. 'Speed' blinks.
Press either the UP or DOWN
key to select the desired
speed (in this case, level 2).
'Speed' continues to blink.
Press the ENTER key. The
display returns to the start
position for the current pipet-
ting mode (in this case, the
standard PIP mode).
1. Bring up the menu
2. Change the
discharging speed
3. Confirm speed
level
Setting the aspiration speed
Setting the discharging speed
SETTING THE ASPIRATION AND DISCHARGING SPEED
What to do How to do it Keys to press Display readout
Setting the Aspiration and Discharging Speed
ErgoOne E -8/-12 · User Manual · 9

1x
1x
CORRECT PIPETTING with the ErgoOne®E -8/-12
1. Attach the tips
Hold the pipette vertically and immerse the tips
2 to 3 mm into the liquid.
Press the pipetting key once
to aspirate the liquid into the
tips. The arrow in the display
points upwards to indicate
the aspiration of liquid.
3. Aspirate liquid
Hold the pipette at an angle between 30º and 45º,
place the tips against the vessel wall.
Press the pipetting key again
and the liquid is completely
discharged including auto-
matic blow-out. Afterwards
wipe pipette tips against the
vessel wall.
4. Discharge liquid After the liquid has been aspirated, the arrow in the
display points downwards to indicate discharging.
5. Eject tips Hold the manifold over a
suitable disposal container
and press the tip ejection
key. Tip ejection
key
The volume is set at the factory to the nominal volume for the ErgoOne®E -8/-12 and can be
changed quickly and easily. See page 8.
Quick start in the standard pipetting mode
Use the correct tips according to the volume range or the color code.
Ensure that the tips are securely seated.
Pipette tips are disposables items!
To avoid the intake of air, leave the tips immersed
into the liquid for approx. 1 sec.
NOTE:
The manifold can turn freely in both direc-
tions.
2. Align the manifold
ISO 8655 prescribes rinsing the pipette tips once with the sample liquid prior
to the actual pipetting process.
NOTE:
10 · ErgoOne E -8/-12 · User Manual

English
THE PIPETTING PROGRAMS
1. Normal Pipetting
PIP Mode
Standard program. A previously set volume
is aspirated into the pipette tip and then
discharged.
12
4. Pipetting for Electrophoresis
GEL Mode
Program for loading electrophoresis gels. A
predefined sample volume is aspirated at high,
adjustable speed and then slowly discharged.
18
2. Mixing of Samples
PIPmix Mode
Program for mixing liquids. The sample is
repeatedly aspirated and discharged.
14
3. Reverse Pipetting
revPIP Mode
Program especially for pipetting liquids with a
high viscosity or vapor pressure, or that tend
to foam.
16
5. Dispensing
DISP Mode
Program for dispensing liquids. An aspirated
volume is dispensed repeatedly in defined
steps.
20
Page
The Pipetting Programs
ErgoOne E-8/-12 · User Manual · 11

3x
1x
1x
1x
➞
Press the MENU key three
times to bring up the mode
selection menu. 'Mode'
blinks.
Use either the UP or DOWN
key to scroll through the
modes until 'PIP' appears.
'Mode' continues to blink.
Press the ENTER key.
The display now shows 'blo'
for blow-out.
Press the pipetting key once
to aspirate the liquid.
Press the pipetting key once
to move the pistons into the
start position.
The arrow in the display
points upwards (aspiration).
PIP MODE The Programs · Settings and Use
The standard program – a previously set volume is aspirated and then discharged.
Volume and speed adjustments are described on pages 8 and 9.
1. Bring up the menu
2. Select PIP mode
3. Confirm PIP mode
4. Prepare for
pipetting
5. Aspirate liquid
What to do How to do it Keys to press Display readout
12 · ErgoOne E -8/-12 · User Manual

1x
1x
➞
1x
English
Press the pipetting key once
to discharge the liquid. The
arrow in the display points
downwards (discharge).
No action required!
When pipetting in the PIP
mode the blow-out function
is performed automatically.
6. Discharge liquid
7. Start blow-out?
Press the ENTER key. The
display shows 'blo'
for blow-out.
1. Bring up the
blow-out function
Press the pipetting key once
to initiate the blow-out pro-
cess. The display moves back
to the start position of the
selected pipetting mode.
2. Start blow-out
START BLOW-OUT MANUALLY
The blow-out function can, if necessary, be initiated manually
at any time.
PIP MODE
What to do How to do it Keys to press Display readout
To accomplish the blow-out, the pistons move to the lowest position.
The user must be certain that any residual liquid is discharged safely.
If the pipetting key is pressed and held, the pistons will be maintained
at their lowest position to avert an accidental aspiration of liquid.
When the key is released, the pistons return to the start position
NOTE:
PIP Mode
ErgoOne E -8/-12 · User Manual · 13

3x
1x
1x
1x
➞
PIPMIX MODE The Programs · Settings and Use
Program for mixing of liquids. The sample is repeatedly aspirated and discharged.
Volume and speed adjustments are described on pages 8 and 9.
Press the MENU key three
times to bring up the mode
selection menu. 'Mode'
blinks.
Scroll through the modes us-
ing either the UP or DOWN
key until 'PIPmix' appears.
'Mode' continues to blink.
Press the ENTER key. The
Display now shows 'blo' for
blow-out.
Press the pipetting key once
to aspirate the liquid.
Press the pipetting key once
to move the pistons into the
start position.
The arrow in the display
points upwards (aspiration).
1. Bring up the menu
2. Select PIPmix
mode
3. Confirm PIPmix
mode
4. Prepare for
pipetting
5. Aspirate liquid
What to do How to do it Keys to press Display readout
14 · ErgoOne E -8/-12 · User Manual

➞
➞
1x
English
Press and hold the pipet-
ting key and the liquid is
alternately aspirated and dis-
charged. The display shows
the up arrow for aspiration
and the down arrow for
discharging and the number
of cycles.
6. Discharge liquid
in the PIPmix
mode
7. End pipetting
The display shows a maximum of 19 cycles.
Press the pipetting key once
and the liquid is discharged
and the blow-out function
initiated.
After the discharge of the
residual liquid (blow-out),
the display moves back to
the start position.
PIPMIX MODE
What to do How to do it Keys to press Display readout
NOTE:
press and hold
PIPmix Mode
ErgoOne E -8/-12 · User Manual · 15

➞
3x
1x
1x
1x
1x
➞
Scroll through the modes us-
ing either the UP or DOWN
key until 'revPIP' appears.
'Mode' continues to blink.
Press the pipetting key once.
The volume aspirated will be
slightly more than set.
To discharge the measured
amount of liquid, press the
pipetting key once. The
arrow in the display points
downwards (discharge).
Some liquid will remain
in the tips.
REVPIP MODE The Programs · Settings and Use
Press the MENU key three
times to bring up the mode
selection menu.
'Mode' blinks.
1. Bring up the menu
2. Select revPIP
mode
Press the ENTER key.
The Display now shows 'blo'
for blow-out.
Press the pipetting key once
to move the pistons into the
start position.
The arrow in the display
points upwards (aspiration).
3. Confirm revPIP
mode
4. Prepare for
pipetting
5. Aspirate liquid
6. Discharge liquid
in the revPIP
mode
Program for pipetting of liquids with high viscosity, vapor pressure or that tend to foam.
Volume and speed adjustments are described on pages 8 and 9.
What to do How to do it Keys to press Display readout
16 · ErgoOne E -8/-12 · User Manual

➞
1x
1x
1x
English
Press the pipetting key again
and the set volume is aspi-
rated into the tips. Press the
pipetting key again and the
volume is discharged again,
and so on...
7. Repeat aspiration
of liquid in revPIP
mode
Press the ENTER key after
the last pipetting operation.
The display shows 'blo' for
blow-out.
8. Initiate blow-out
Press the pipetting key once
to initiate the blow-out
process. The residual liquid is
discharged.
After the residual liquid is
discharged (blow-out), the
display moves back to the
start position.
9. End pipetting
REVPIP MODE
What to do How to do it Keys to press Display readout
revPIP Mode
ErgoOne E -8/-12 · User Manual · 17

1x
➞
1x
1x
3x
Press the MENU key three
times to bring up the mode
selection menu.
'Mode' blinks.
Scroll through the modes
using the arrow keys until
'GEL' appears.
'Mode' continues to blink.
Press the ENTER key.
The Display now shows 'blo'
for blow-out.
Press the pipetting key once.
The set volume is aspirated
into the tips.
1. Bring up the menu
2. Select GEL mode
3. Confirm GEL mode
5. Aspirate liquid
Press the pipetting key once
to move the pistons into the
start position.
The arrow in the display
points upwards (aspiration).
4. Prepare for
pipetting
Aspirate a larger volume
In order to aspirate a larger
volume than was set (up
to a max. of 110% of the
nominal volume), press
and hold the pipetting key
until the desired volume
has been aspirated. The
display shows a rhombus.
ELECTROPHORESIS (GEL) MODE The Programs · Settings and Use
Program for loading electrophoresis gels. A predefined sample volume is aspirated into
the pipette tips with high adjustable speed and then slowly discharged.
Volume and speed adjustment is described on pages 8 and 9.
What to do How to do it Keys to press Display readout
press and hold
18 · ErgoOne E -8/-12 · User Manual

➞
1x
1x
1x
1x
English
Press the pipetting key once
to discharge the liquid. The
rhombus is shown in the dis-
play. The liquid is discharged
very slowly.
6. Discharge liquid
in the GEL mode
Interrupt discharging
To interrupt discharging,
press the pipetting key
again. The display shows the
volume discharged prior to
interruption.
Press the ENTER key after
the last pipetting operation.
The display shows 'blo' for
blow-out.
7. Initiate blow-out
Press the pipetting key once
to initiate the blow-out
process. The residual liquid is
discharged.
After the residual liquid is
discharged (blow-out), the
display moves back to the
start position.
8. End pipetting
ELECTROPHORESIS (GEL) MODE
What to do How to do it Keys to press Display readout
The GEL mode operates using a very slow discharge speed to prevent swirling of the samples. To assure
optimal discharging into a gel, this discharge speed is fixed for the GEL mode. This speed is significantly
slower than level 1 and cannot be selected individually.
NOTE:
Electrophoresis (GEL) Mode
ErgoOne E -8/-12 · User Manual · 19

3x
1x
1x
+
–
1x
Scroll through the modes us-
ing either the UP or DOWN
key until 'DISP' appears.
‚Mode‘ continues to blink.
Press the UP or DOWN key
to set the volume. Pressing
and holding the key ac-
celerates the rate of change.
'VOL' blinks.
Press the ENTER key.
The display now shows the
new volume setting for the
dispensing steps and the
max. number of steps.
'Steps' blinks.
DISP MODE The Programs · Settings and Use
Press the MENU key three
times to bring ap the mode
selection menu.
'Mode' blinks.
1. Bring up the menu
2. Select DISP
mode
Press the ENTER key.
The Display now shows
'blo' for blow-out.
Press the pipetting key once
to move the pistons into the
start position.
The arrow in the display
points upwards (aspiration).
3. Confirm DISP
mode
4. Prepare for
dispensing
5. Set dispensing
step volume
6. Confirm dispens-
ing step volume
Program for discharging an aspirated liquid in pre-defined steps.
The volume aspirated will be a little bit more than actually needed.
Speed adjustment is described on page 9.
What to do How to do it Keys to press Display readout
20 · ErgoOne E -8/-12 · User Manual