Thermo Scientific GENESYS 10S Vis User manual

GENESYS 10S Vis
User Guide
269-251700 Revision A October 2009

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Thermo Scientific GENESYS 10S Vis User Guide iii
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Chapter 1 Spectrophotometer Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Spectrophotometer Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
About the Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Cell Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
6-Position Cell Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Single Cell Holder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Selecting and Positioning Cuvettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Z-dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Chapter 2 Setting Up the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Entering Parameter Values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Numeric Entry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Menu Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
On/Off Toggle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Alphanumeric Entry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Setting Utility Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Setting the Date and Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Setting Baseline Expiration Time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Setting the Screen Contrast . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Setting Up the Internal Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Setting the Utility Parameters for the Printer. . . . . . . . . . . . . . . . . . . . . . . . . 12
Chapter 3 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Cell Holders and Cell Holder Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Cell Holder Configurations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Cell Holder Initialization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Changing Cell Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Installing the 6-Position Cell Holder and the Single Cell Holder. . . . . . . . . . 19
Removing the 6-Position Cell Holder and the Single Cell Holder . . . . . . . . . 20
Installing Accessory Cell Holders. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Installing the Internal Printer (Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Loading Paper in the Internal Printer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Contents

Contents
iv GENESYS 10S Vis User Guide Thermo Scientific
External Printers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Chapter 4 Sample Positioner Setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
Auto 6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Auto 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Single Cell Holder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Manual 6. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Chapter 5 Cell Correction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
Cell Correction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Specifying Wavelengths for Discrete nms Mode . . . . . . . . . . . . . . . . . . . . . . 29
Chapter 6 Managing Stored Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Software Password. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Naming a Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Saving a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Loading Test Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Lock/Unlock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Deleting a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Chapter 7 SmartStart. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
Chapter 8 Concentration Units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
Specifying Concentration Units. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Creating Custom Units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Chapter 9 Calculator Function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
Chapter 10 Abs and %T Measurements—Basic A-%T-C . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
Setting the Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Measuring a Blank. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Measuring Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Chapter 11 Abs and %T Measurements—Advanced A-%T-C. . . . . . . . . . . . . . . . . . . . . . . . . .45
Recalling a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Setting Up Test Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Taking Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Chapter 12 Basic Concentration Measurements—Basic A-%T-C. . . . . . . . . . . . . . . . . . . . . .49
Basic Concentration Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Setting the Wavelength and Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Using Conc/Std to Measure Concentration. . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Using Conc/Factor to Measure Concentration . . . . . . . . . . . . . . . . . . . . . . . . . 51
Measuring Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Contents
Thermo Scientific GENESYS 10S Vis User Guide v
Chapter 13 Concentration Measurements—Advanced A-%T-C. . . . . . . . . . . . . . . . . . . . . . . .53
Recalling a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Setting Up Test Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Measuring a Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Entering a Factor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Measuring Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Chapter 14 Scanning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Recalling a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Setting Up Test Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Collecting a Baseline Scan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Scanning a Sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Viewing and Manipulating Scan Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Rescaling Graphical Scan Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Performing Calculations on the Scan Data . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Labeling Peaks and Valleys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Smoothing Data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Determining Peak Height Using a 3-point Net Equation. . . . . . . . . . . . . . 63
Calculating the Area Under a Curve. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Viewing and Rescaling Tabular Scan Data. . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Chapter 15 Multiwavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
Recalling a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Setting Up Test Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Adding Wavelengths and Factors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Deleting Wavelengths and Factors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Taking Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Chapter 16 Absorbance Ratio . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
Recalling a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Setting Up Test Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Measuring Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Chapter 17 Absorbance Difference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75
Recalling a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Using the Absorbance Ratio Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Setting Up Test Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Measuring Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Chapter 18 3-Point Net . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
Recalling a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Setting Up Test Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Taking Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

Contents
vi GENESYS 10S Vis User Guide Thermo Scientific
Chapter 19 Concentration Measurements—Standard Curve Application . . . . . . . . . . . . . . .83
Recalling a Standard Curve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Setting the Parameters for a Standard Curve . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Measuring the Standards for a Standard Curve . . . . . . . . . . . . . . . . . . . . . . . . . 84
Using the Standards Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Measuring Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Editing a Standard Curve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Chapter 20 Kinetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
Recalling a Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Setting Up Test Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Measuring Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Recalling and Recalculating Graphical Kinetics Results . . . . . . . . . . . . . . . . . . . 94
Rescaling and Recalculating Tabular Kinetics Results . . . . . . . . . . . . . . . . . . . . 97
Chapter 21 Performance Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99
Accessing the Performance Verification Tests . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Troubleshooting Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Wavelength Accuracy - Internal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Wavelength Accuracy - Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Resolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Photometric Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Selecting the Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Adding Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Deleting Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Running the Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Noise. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Stray Light. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Running the Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Internal Printer Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Chapter 22 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109
Routine Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Cleaning and Maintaining Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
Cleaning the Windows of the Sample Compartment. . . . . . . . . . . . . . . . . . 112
Changing the Fuse. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Replacing the Tungsten Lamp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Chapter 23 Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117

Thermo Scientific GENESYS 10S Vis User Guide vii
P
Preface
Congratulations on your purchase of a Thermo Scientific spectrophotometer! Our
spectrophotometers integrate advanced hardware features with the power and flexibility of a
wide range of accessories.
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
Note Notes contain helpful supplementary information.
IMPORTANT Follow instructions labeled “Important” to avoid damaging the system
hardware or losing data.
CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or
moderate injury.
WARNING Indicates a hazardous situation which, if not avoided, could result in death or
serious injury.

Preface
viii GENESYS 10S Vis User Guide Thermo Scientific

Thermo Scientific GENESYS 10S Vis User Guide 1
1
Spectrophotometer Basics
This chapter describes:
•Spectrophotometer Components
•Cell Holders
•Selecting and Positioning Cuvettes
•Z-dimensions
Spectrophotometer Components
Here are some major components visible on the outside of a typical instrument:
Connectors
The connectors are on the back of the instrument:
Keypad
Optional printer housing
Sample compartment
USB port

1Spectrophotometer Basics
Spectrophotometer Components
2GENESYS 10S Vis User Guide Thermo Scientific
WARNING Avoid shock hazard. Always turn off the instrument and unplug it from the
wall outlet or power strip before you unplug the power cord from the instrument
connector.
USB communication port USB printer port
On/Off switch
A/C power connector
Fuse compartment

1 Spectrophotometer Basics
Spectrophotometer Components
Thermo Scientific GENESYS 10S Vis User Guide 3
About the Keypad
Key or button Function
Function keys
• Called “Function” keys.
• Performs a specific function as displayed above each key.
• Functions will change depending on the software screen.
• Some function keys may not be active.
• Clears the value being entered.
• Returns to the previous screen.
• Deletes the last character entered.

1Spectrophotometer Basics
Cell Holders
4GENESYS 10S Vis User Guide Thermo Scientific
Cell Holders
Your instrument includes a 6-position and single cell holder.
• Accepts highlighted, entered, or selected values.
• Advances to the next parameter or screen.
• Prints the method or results to the selected printer.
•If“PC”
is selected for the printer, sends the method or results
to the USB port.
• Displays a menu of software applications.
• Displays the Utility screen.
• Controls the location of the cursor
• Highlights the value or option for the selection.
• Enters numbers, a decimal point and a minus sign for values.
• Cell position keys.
• Selects the cell holder position to be measured.
• B = blank and 1-5 = sample positions.
• = positions when used in Auto 3 mode.
Key or button Function

1 Spectrophotometer Basics
Selecting and Positioning Cuvettes
Thermo Scientific GENESYS 10S Vis User Guide 5
6-Position Cell Holder
Single Cell Holder
See the parts list for a more detailed list of available accessories.
Selecting and Positioning Cuvettes
The compatible wavelength range for different types of cells depends on the material used.
Note If the Cell Positioner method option is set to Auto 6, whenever you press Run Test
to start a measurement, the instrument attempts to initialize the cell positioner. If a single
cell holder is installed, the message “Error, Single Cell Holder found. Use Single Cell
Holder?” appears. Press Accept Change to continue the measurement with a single cell, or
install the 6-cell changer and press Cancel Change.
Cell Type Wavelength
Optical Glass
Borosilicate Glass
360 nm to > 1100 nm
330 nm to > 1100 nm
Disposable:
Quartz
Polystyrene
Methacrylate
Acrylic
UV-transparent
190 nm to > 1100 nm
> 340 nm
> 300 nm
> 280 nm
> 220 nm
Note See the manufacturer’s specifications and work within the recommended range.

1Spectrophotometer Basics
Z-dimensions
6GENESYS 10S Vis User Guide Thermo Scientific
Other Guidelines
Position cuvettes and test tubes so that the clear sides face the light beam, one clear side facing
the front of the instrument and the other facing the back.
When using small aperture (small volume) cells:
• Always used cells with black masking
• Use the same cell (or cuvette) for your blank and your samples
Z-dimensions
The figure below illustrates the position of the light beam in the instrument.
Beam size specifications are shown below.
• Distance from bottom of cuvette to center of beam (Z-dimension): 8.5 mm
• Beam dimensions: 2 mm (wide) by 7 mm (tall)
Note The pathlength of test tubes is not as well defined as that of square cuvettes.
Note Always place test tubes in the instrument in exactly the same orientation in the light
beam. An alignment mark on the test tube helps you orient the test tubes consistently and
correctly.

Thermo Scientific GENESYS 10S Vis User Guide 7
2
Setting Up the Instrument
Setting up the instrument involves:
•Entering Parameter Values
•Setting Utility Parameters
•Setting Up the Internal Printer
Entering Parameter Values
The following sections describe the use of the keypad to interact with menus and controls.
These sections provide instructions for:
•Numeric Entry
•Menu Selection
•On/Off Toggle
•Alphanumeric Entry
Numeric Entry
With the parameter (e.g., Wavelength) highlighted, start typing the numeric value. An Entry
window with the value range appears. Type the complete entry and press Enter.

2Setting Up the Instrument
Entering Parameter Values
8GENESYS 10S Vis User Guide Thermo Scientific
Alternatively, you can press Enter to display the Entry window with the value range and then
type the complete entry and press Enter.
Menu Selection
With the parameter (Units, or Sample Positioner) highlighted, press Enter to display the
selection list. Highlight the appropriate item and press Enter.
On/Off Toggle
With the parameter (e.g., AutoPrint) highlighted, press Enter to toggle to the opposite value.
Alphanumeric Entry
With the parameter (e.g., Test Name) highlighted, press Enter. The Name Entry screen
appears. Highlight the desired character and press Add Character. When you are finished,
press Accept Name.

2 Setting Up the Instrument
Setting Utility Parameters
Thermo Scientific GENESYS 10S Vis User Guide 9
Setting Utility Parameters
The Utility menu lets you set certain non-test hardware parameters, such as the date and time,
standby setting, screen contrast settings and printer setup. You can also access a directory of all
stored tests and the calculator function.
You cannot set utility parameters or change the utility when the instrument is carrying out a
measurement.
•PressUtility on the keypad.
Setting the Date and Time
Highlight Date/Time Setup and press Enter.
You can modify the date, time format and time.
YTo set the date
1. Highlight Set Date and press Enter.

2Setting Up the Instrument
Setting Utility Parameters
10 GENESYS 10S Vis User Guide Thermo Scientific
2. Press Set Date, type the date and press Enter.
3. Press Set Month, highlight the correct month and press Enter.
4. Press Set Year, type the year and then Enter.
5. Press Esc to save the settings.
YTo select the time format
You can set the instrument to display the time in either am/pm or 24-hour format. To change
the format, highlight Time Format and press Enter until the desired format (AM/PM or 24
hour) appears.
YTo set the time
1. Highlight Set Time and press Enter.
2. To set the hour, press Set Hour, type in the hour and press Enter.
3. To set the minutes, press Set Minute, type in the minute and press Enter.
4. To select between AM and PM (if in AM/PM time format), press Set AM/PM until the
appropriate setting appears.
Since the tungsten lamp of the GENESYS 10S Vis spectrophotometer requires a 30-minute
warm-up, the spectrophotometer has been pre-set at the factory to Standby Mode OFF.
YTo change Standby Mode time
1. Highlight Standby and press Enter.
2. Highlight the length of time you want the instrument to wait before entering standby
mode and press Enter.
Note Your changes are saved automatically (even during power down) by battery
backup.

2 Setting Up the Instrument
Setting Utility Parameters
Thermo Scientific GENESYS 10S Vis User Guide 11
Setting Baseline Expiration Time
If you will be performing scans on your samples, you can set a time limit for which a collected
baseline will be valid. This is particularly useful when measurements are made in a production
setting across multiple shifts or when the nature of the blank sample changes dramatically
with time.
YTo set the baseline expiration time
1. Highlight Baseline Expiration (hr:min) and press Enter.
2. Enter the desired time in the Entry baseline expiration time field and press Enter.
Setting the Screen Contrast
To make it easier to read the display, you can adjust the screen contrast on the instrument.
YTo set the screen contrast
1. Highlight Screen Contrast and press Enter.

2Setting Up the Instrument
Setting Up the Internal Printer
12 GENESYS 10S Vis User Guide Thermo Scientific
2. Adjust the contrast by following the instructions on the screen.
3. Press Esc.
Setting Up the Internal Printer
To set up the internal printer, you need to set its internal parameters and load the paper.
YTo set up the internal printer
1. Install the internal printer.
If you ordered the internal printer as a separate item, you need to install it. See “Installing
the Internal Printer (Optional)” on page 21 section in Accessories for instructions.
2. Load paper in the printer.
See “Loading Paper in the Internal Printer” on page 22 in Accessories for instructions on
installing the printer.
Setting the Utility Parameters for the Printer
Paper printouts are available from both the internal printer and a USB printer attached to the
instrument. Alternatively, displayed ASCII text and graphics can be sent to a computer using
a USB connection.
Enabling the PC as the printing device sends ASCII data to the PC via the USB connection to
the PC. No graphics are sent. A program on the PC is required to capture and use the data
(not provided).
To ensure that the instrument can output information correctly to the printer, select the
appropriate device.
YTo set the utility parameters for the printer
1. Press Utility.
2. Highlight Printer and press Enter.