Thermo Scientific BioCane CK50900 Series User manual

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User Manual
BioCane™ Type CK50900
Cryobiological Storage Vessel
Operation Manual and Parts List LT509X17 (7000807) Rev. 10

Thermo Scientific
Manual Number LT509X17 (7000807)
10 EC M-000831 02/25/21 Removed Warranty caution from preface page ii and 1-1. Added Safety Precautions
in Preface page iii, WEEE Compliance in page 6-1 and removed
EUT statements in page 2-1. Updated Specifications for Environmental
Conditions in page 2-1. TFI
9 41864 11/07/17 Removed CE mark, number bpg
8 41238 12/20/16 Combined languages to one file slb
7 40578 2/23/16 Clarified Do ot Roll warning ccs
6 -- 10/27/15 Updated symbols and labels, added cautions ccs
5 30559 11/12/13 Added warnings and cautions to pg 1-2, environmental conditions to pg 2-1 ccs
4 27797 9/21/11 Added manufacturer icon and euro rep ccs
3 27547 7/28/11 Updated specs - BioCane 73 vials from 2880 to 2016 ccs
2 27457/SI-10618 7/21/11 Added CE number to pg 2-1 ccs
1 -- 2/24/11 Added part numbers for replacement canisters ccs
0 -- 5/28/10 Transfer to Marietta (was LT509X17 8/12/09) ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Cryobiological Storage Vessel i
Catalog Number Model Number Name
CK509X2 807 BioCane 20
CK509X3 808 BioCane 34
CK509X4 809 BioCane 47
CK509X6 820 BioCane 73

Thermo Scientificii Cryobiological Storage Vessel
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow instructions in this
manual may result in damage to the unit, injury to operating personnel, and poor equipment
performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Warning Nitrogen gas can cause suffocation without warning. Store and use liquid nitrogen only in a
well-ventilated place. As the liquid evaporates, the resulting gas displaces the normal air in the area.
(The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture, not
the gas itself. The issuing gas is invisible.) n closed areas, excessive amounts of nitrogen gas reduces the
concentration of oxygen and can result in asphyxiation. Because nitrogen gas is colorless, odorless, and
tasteless, it cannot be detected by the human senses. Breathing an atmosphere that contains less than
19.5% oxygen can cause dizziness and quickly result in unconsciousness and death. Therefore, the use
of oxygen monitoring equipment is strongly recommended. s
Warning Contact of liquid nitrogen with the skin or eyes may cause serious (freezing) injury. s
Warning Do not roll. Always keep liquid nitrogen vessel in an upright position. Exercise caution to
prevent spilling and splashing. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. n no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
Intended Use:
The Thermo Fisher Scientific cryogenic storage vessels are intended to store biological samples at cryogenic
temperatures. These products are comprised of a vessel and a lid, with or without a level monitor.
Unintended Use:
These products are not intended for use within the patient environment.
When this manual is translated into other languages, the US English version is binding.
© 2021 Thermo Fisher Scientific. All rights reserved. Printed in U.S.A.

Thermo Scientific Cryobiological Storage Vessel iii
Preface
mportant operating and/or maintenance instructions. Read the accompanying text carefully.
Extreme temperature hazards. Only qualified persons should perform procedures associated with this
symbol.
Potential biological hazards. Proper protective equipment and procedures must be used.
This symbol when used alone indicates important operating instructions which reduce the risk of
injury or poor performance of the unit.
CAUTION: This symbol, in the context of a CAUT ON, indicates a potentially hazardous situation
which if not avoided could result in minor to moderate injury or damage to the equipment. s
CAUTION: This indicates a situation which may result in property damage. s
Before installing, using or maintaining this product, please be sure to read the manual and product
warning labels carefully. Failure to follow these instructions may cause the product to malfunction,
which could result in injury or damage.
WARNING: Unauthorized repair of your freezer will invalidate your warranty. Contact Technical
Service at 1-800-438-4851 for additional information. s

Thermo Scientificiv Cryobiological Storage Vessel
Preface
Do You Need Information or Assistance on
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Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
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Regardless of your needs, our professional telephone technicians are available to assist you
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telephone or fax. If you wish to write, our mailing address is:
Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road, Box 649
Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.

Cryobiological Storage Vessel vThermo Scientific
Table of Contents
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Please Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Measuring Liquid Nitrogen Quantity . . . . . . . . . . . . . . . . . . . . . . . .3-2
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
WEEE Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6

vi Cryobiological Storage Vessel Thermo Scientific
Table of Contents

Cryobiological Storage Vessel 1-1Thermo Scientific
Please Read
Section 1 Safety Information
This manual contains important operating and safety information.
The user must carefully read and understand the contents of this manual
prior to the use of this equipment.
Your Thermo Scientific cryogenic vessel has been designed with function,
reliability, and safety in mind. t is the user’s responsibility to install it in
conformance with local electrical codes. For safe operation, pay attention
to the warnings and cautions throughout the manual.
All cryopreservation vessels, regardless of who manufactures them, use a
vacuum to provide for the super insulative properties needed to keep
nitrogen in a liquid form. They are very similar, in fact, to the thermos
vessels you may have used to store coffee, soup or milk.
Remember how fragile they were? Since no vacuum is perfect or will last
forever, we suggest that you monitor the consumption of liquid nitrogen
used by your vessel on a regular basis, i.e. every 1-2 days. By monitoring,
you may be able to anticipate subtle changes in consumption and possibly
react to problems before they arise. You may opt to use a level monitor for
this purpose.
Also, if your samples are super-critical, one-of-a-kind, or irreplaceable,
consider allocating your samples into 2 separate vessels.
Remember the old adage about placing all your eggs into one basket…
Warnings
• This unit is intended to be used by trained personnel in a
well-ventilated environment.
• Liquid nitrogen is extremely cold; it boils at -196°C.
• Follow all necessary precautions and warnings relevant to applicable
hazards such as frostbite, contact burn, asphyxiation, etc., in cryogenic
areas.

1-2 Cryobiological Storage Vessel Thermo Scientific
Section 1
Safety Information
• To avoid injury due to frostbite or ruptured vials, use extreme care
whenever handling liquid nitrogen, liquid nitrogen storage or transfer
vessels or any objects which have come in contact with liquid
nitrogen.
• Leave no areas of skin exposed.
• Wear adequate PPE where necessary: face shield, cryogenic gloves,
cryogenic apron.
• Only use the power supply provided with the unit.
• Do not tightly seal liquid nitrogen containers or prevent nitrogen gas
from escaping.
• Always handle vials with tongs.
• Use extreme care to prevent spilling and splashing liquid nitrogen
during transfer and removal of storage contents and holders.
• mmediately remove any clothing or safety attire on which liquid
nitrogen has been spilled or splashed .
• Get immediate medical attention for any frostbite injuries due to
liquid nitrogen.
• Never overfill liquid nitrogen vessels.
Use this product only in the way described in the product literature•
and in this manual. Before using it, verify that this product is suitable
for its intended use. f the equipment is used in a manner not
specified by the manufacturer, the protection provided by the
equipment may be impaired.
When inserting or removing cans, be careful not to come in contact
with the neck tube area of the vessel. Remove or insert cans slowly,
in a vertical manner. Scratches on the neck tube can cause remature
vacuum failure.
Do not tam er with, or remove vacuum ort (covered by black
lastic ca on side of vessel); this will void warranty.
Do not spill liquid nitrogen on vacuum port - this can cause vacuum
failure.
Always keep liquid nitrogen in an upright position.

Cryobiological Storage Vessel 2-1Thermo Scientific
Section 2 General Specifications
* Actual working time may vary due to current atmospheric conditions, container
history, manufacturing tolerances, and any individual patterns of use.
Environmental Conditions
Operating temperature: -29°C to +60°C
10% to 95% relative humidity, non-condensing.
Altitude limit: 2,000 meters.
Storage: -29°C to +60°C; 10% to 95% relative humidity.
ntended for indoor use only.
BioCane 20 BioCane 34 BioCane 47 BioCane 73
Height (Inches) 25.70" 26.60" 26.50" 27.00"
Diameter (Inches) 14.50" 18.20" 20.00" 22.00"
L 2 Capacity 20.50 L 34.80 L 47.40 L 73.0 L
Static Holding Time (Days) 227 193 121 121
Static L 2 Evaporation Rate* 0.1 L/day 0.18 L/day 0.40 L/day 0.60 L/day
Liquid Phase Capacity 1.2 ml 180 vials 720 vials 1512 vials 2016 vials

2-2 Cryobiological Storage Vessel Thermo Scientific
Section 2
General Specifications
Unpacking Before using your new vessel, carefully inspect it. Check for signs of
damage which may have occurred in shipment. t is advisable to fill (see
Filling nstructions) all new units with liquid nitrogen and watch liquid
nitrogen loss rate for a few days. f there are any problems, call Technical
Services as soon as possible.
The on-line warranty registration address is listed on the front of this
manual. The unit should be registered within 30 days to make warranty
effective. This information must include the serial number which is located
on the handle of the vessel.
Note The most prevalent cause of failure of liquid nitrogen storage vessels
is mechanical. The vessel neck tube supports the full weight of the inner
shell and all liquid nitrogen it contains. A side or corner blow to the vessel
causes the inner shell to swing in a pendulum motion causing the neck
tube to be damaged. Any storage vessel which has been exposed to an
accident, has been dropped or lowered to hit on one corner will tend to fail
more rapidly than one which has not.
Caution Exercise caution when moving your BioCane™ vessel. BioCane
cryobiological storage systems are not transportation vessels. Transport
carts are designed for mobility within the lab or lab to lab only. Moving
full vessels long distances, over cracks in floor, thresholds, on inclined
ramps or in elevators can cause premature vacuum failure.
Note f samples must be transported under cryogenic conditions, consider
the Thermo Scientific Arctic Express line of Dry Shippers.

Cryobiological Storage Vessel 3-1Thermo Scientific
Section 3 Operation
To avoid damage to your cryogenic storage vessel which may result in
premature vacuum loss, it is important that the following procedure be
used during the addition of liquid nitrogen to a warm vessel:
1. Add only a small amount of liquid nitrogen (2 - 10 liters) to new or
warm vessels.
2. Allow this small amount of liquid nitrogen to sit in the covered vessel
for a minimum of 2 hours. This will limit stress caused by the sudden
temperature change associated with adding liquid nitrogen to a warm
vessel.
3. Add an additional 2 - 10 liters LN2 to vessel.
4. Allow vessel to sit for 48 hours and monitor liquid nitrogen
consumption.
5. Fill vessel as desired. Remember to allow for displacement of liquid
nitrogen when cannisters and canes are inserted.
6. nsert and remove cannisters slowly. Allow liquid nitrogen to run out
of cannisters.
Caution Never overfill liquid nitrogen vessels.
When inserting or removing cans, be careful not to come in contact with
the neck tube area of the vessel. Remove or insert cans slowly, in a
vertical manner. Scratches on the neck tube area can cause remature
vacuum failure.
Do not spill liquid nitrogen on vacuum port - this can cause vacuum
failure. s

3-2 Cryobiological Storage Vessel Thermo Scientific
Section 3
Operation
Measuring Liquid
Nitrogen uantity
1. Use a wooden yardstick to measure liquid nitrogen level. Never use a
hollow tube or plastic dipstick to measure liquid nitrogen level.
2. Level will be indicated by frost line which develops when dipstick is
removed and waved in a back and forth motion away from the user.

Cryobiological Storage Vessel 4-1Thermo Scientific
Section 4 Replacement Parts
Part Number Description
AY509X13 alge Cyrovials 1.2 ml, 500/case
AY509X12 alge Cryovials 2.0 ml, 500/case
AY509X20 CryoCane (aluminum) - each cane holds 5 vials for storage in BioCane cannisters (48/case)
AY509X21 CryoSleeve enclosures - Clear plastic sleeve fits over CryoCane for added security during handling or storage (100/case)
CS509X17 algene System 100-ampule box accommodates most internally threaded 1.2 and 2.0 ml vials
CS509X18 algene System 100-ampule box accommodates most internally threaded 1.2 and 2.0 ml vials
AY509X32 algene System 100 Cyrogenic Vials - 1 ml (500/case)
AY509X33 algene System 100 Cryogenic Vials - 1.5 ml (500/case)
AY509X34 algene System 100 Cryogenic Benchtop Vial Holder (1/package)
AY509X35 algene System 100 Cryogenic Benchtop Vial Holder (1/package)
CryoCane coders - Aluminum identification tabs that slip over the end of a CryoCane to identify the cane written on for further
identification (100/case)
AY509X22 White
AY509X23 Yellow
AY509X24 Blue
AY509X25 Green
AY509X26 Red
Cryovial closure color coders - Plastic disks that fit into the top of Nalge Cryovials to identify individual (100/package)
AY509X27 White
AY509X28 Yellow
AY509X29 Blue
AY509X30 Green
AY509X31 Red

4-2 Cryobiological Storage Vessel Thermo Scientific
Section 4
Replacement Parts
Refer to the Specification Plate for the complete model number, serial
number, and series number when requesting service, replacement parts or
in any correspondence concerning this unit.
All parts listed herein may be ordered from the Thermo Scientific dealer
from whom you purchased this unit or can be obtained promptly from the
factory.When service or replacement parts are needed, check first with your
dealer. f the dealer cannot process your request, then contact our
Technical Services Department.
Prior to returning any materials, contact our Technical Services
Department for a “Return Materials Authorization” number (RMA).
Material returned without an RMA number will be refused.
Ordering Procedures
Part Number Description
HR509X25 BioCane 20 (CK509X2) [807]) replacement canister
HR509X26 BioCane 34 (CK509X3 [808]) replacement canister
HR509X27 BioCane 47 (CK509X4 [809]) replacement canister
HR509X28 BioCane 73 (CK509X6 [820]) replacement canister

Cryobiological Storage Vessel 5-1Thermo Scientific
THERMO FISHER SCIENTIFIC STANDARD PRODUCT WARRANTY
(LN2Vacuum)
The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protec-
tion extends to any subsequent owner during the first year warranty period.
During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced
at Thermo's expense, labor included. LN2Vacuum Integrity is covered for five years. Installation and calibration are not
covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.
Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give prior approval for return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to
Thermo postage paid and replacement parts are shipped FOB destination. THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or
1-740-373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service and
special application. Outside the USA, contact your local distributor for warranty information.
Rev. 0 5/10
ISO
9001
REGISTERED
Section 5
Warranty Information

5-2 Cryobiological Storage Vessel Thermo Scientific
Section 5
Warranty Information

Cryobiological Storage Vessel 6-1Thermo Scientific
Section 6 WEEE Compliance
WEEE Compliance. This product is required to comply Great Britain
with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2012/19/EU. t is marked
with the following symbol. Thermo Fisher Scientific has con-
tracted with one or more recycling/disposal companies in
each EU Member State, and this product should be disposed
of or recycled through them. Further information on our
compliance with these Directives, the recyclers in your
country, and information on Thermo Scientific products
which may assist the detection of substances subject to the
RoHS Directive are available at www.thermofisher.com/
WEEE Konformittät. Dieses Produkt muss die EU Waste Deutschland
Electrical & Electronic Equipment (WEEE) Richtlinie
2012/19/EU erfüllen. Das Produkt ist durch folgendes
Symbol gekennzeichnet. Thermo Fisher Scientific hat
Vereinbarungen getroffen mit Verwertungs-
/Entsorgungsanlagen in allen EU-Mitgliederstaaten und
dieses Produkt muss durch diese Firmen widerverwetet oder
entsorgt werden. Mehr nformationen über die Einhaltung
dieser Anweisungen durch Thermo Scientific, dieVerwerter
und Hinweise die hnen nützlich sein können, die Thermo
Fisher Scientific Produkte zu identizfizieren, die unter diese
RoHS. Anweisungfallen,
finden Sie unter www.thermofisher.com/
Conformità WEEE. Questo prodotto deve rispondere alla Italia
direttiva dell’ Unione Europea 2012/19/EU in merito ai
Rifiuti degli Apparecchi Elettrici ed Elettronici (WEEE).
marcato col seguente simbolo. Thermo Fischer Scientific ha
stipulato contratti con una o diverse società di
riciclaggio/smaltimento in ognuno degli Stati Membri
Europei. Questo prodotto verrà smaltito o riciclato tramite
queste medesime. Ulteriori informazioni sulla conformità di
Thermo Fisher Scientific con queste Direttive, l’elenco delle
ditte di riciclaggio nel Vostro paese e informazioni sui
prodotti Thermo Scientific che possono essere utili alla
rilevazione di sostanze soggette alla Direttiva RoHS sono
disponibili sul sito http://www.thermofisher.com/
WEEE Compliance

6-2 Cryobiological Storage Vessel Thermo Scientific
Section 6
WEEE Compliance
WEEE Compliance
(cont.)
Conformité WEEE. Ce produit doit être conforme France
à la directive euro-péenne (2012/19/EU) des Déchets
d’Equipements Electriques et Electroniques (DEEE). l est
marqué par le symbole suivant. Thermo Fisher Scientific
s’est associé avec une ou plusieurs compagnies de recyclage
dans chaque état membre de l’union européenne et ce
produit devraitêtre collecté ou recyclé par celles-ci.
Davantage d’informations sur laconformité de Thermo
Fisher Scientific à ces directives, les recycleurs dans votre
pays et les informations sur les produits Thermo Fisher
Scientific qui peuvent aider le détection des substances
sujettes à la directive RoHS sont disponibles sur
http://www.thermofisher.com/
Cumplimiento de WEEE. Se requiere que este producto S anish
cumpla con la Directiva 2012/19/EU de Desechos de
Equipos Eléctricos y Electrónicos (WEEE) de la Unión
Europea. Está marcado con el siguiente símbolo. Thermo
Fisher Scientific ha contratado a una o más empresas de
reciclaje / eliminación en cada Estado miembro de la UE, y
este producto debe desecharse o reciclarse a través de ellos.
Para obtener más información sobre el cumplimiento de estas
Directivas, los recicladores de su país e información sobre los
productos Thermo Scientific que pueden ayudar a detectar
sustancias sujetas a la Directiva RoHS,
visite www.thermofisher.com/

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