Thermo Scientific BioCane CK50900 Series User manual

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User Manual
BioCane™ Type CK50900
Cryobiological Storage Vessel
Operation Manual and Parts List LT509X17 (7000807) Rev. 10

Thermo Scientific
Manual Number LT509X17 (7000807)
10 EC M-000831 02/25/21 Removed Warranty caution from preface page ii and 1-1. Added Safety Precautions
in Preface page iii, WEEE Compliance in page 6-1 and removed
EUT statements in page 2-1. Updated Specifications for Environmental
Conditions in page 2-1. TFI
9 41864 11/07/17 Removed CE mark, number bpg
8 41238 12/20/16 Combined languages to one file slb
7 40578 2/23/16 Clarified Do ot Roll warning ccs
6 -- 10/27/15 Updated symbols and labels, added cautions ccs
5 30559 11/12/13 Added warnings and cautions to pg 1-2, environmental conditions to pg 2-1 ccs
4 27797 9/21/11 Added manufacturer icon and euro rep ccs
3 27547 7/28/11 Updated specs - BioCane 73 vials from 2880 to 2016 ccs
2 27457/SI-10618 7/21/11 Added CE number to pg 2-1 ccs
1 -- 2/24/11 Added part numbers for replacement canisters ccs
0 -- 5/28/10 Transfer to Marietta (was LT509X17 8/12/09) ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Cryobiological Storage Vessel i
Catalog Number Model Number Name
CK509X2 807 BioCane 20
CK509X3 808 BioCane 34
CK509X4 809 BioCane 47
CK509X6 820 BioCane 73

Thermo Scientificii Cryobiological Storage Vessel
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow instructions in this
manual may result in damage to the unit, injury to operating personnel, and poor equipment
performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Warning Nitrogen gas can cause suffocation without warning. Store and use liquid nitrogen only in a
well-ventilated place. As the liquid evaporates, the resulting gas displaces the normal air in the area.
(The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture, not
the gas itself. The issuing gas is invisible.) n closed areas, excessive amounts of nitrogen gas reduces the
concentration of oxygen and can result in asphyxiation. Because nitrogen gas is colorless, odorless, and
tasteless, it cannot be detected by the human senses. Breathing an atmosphere that contains less than
19.5% oxygen can cause dizziness and quickly result in unconsciousness and death. Therefore, the use
of oxygen monitoring equipment is strongly recommended. s
Warning Contact of liquid nitrogen with the skin or eyes may cause serious (freezing) injury. s
Warning Do not roll. Always keep liquid nitrogen vessel in an upright position. Exercise caution to
prevent spilling and splashing. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. n no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
Intended Use:
The Thermo Fisher Scientific cryogenic storage vessels are intended to store biological samples at cryogenic
temperatures. These products are comprised of a vessel and a lid, with or without a level monitor.
Unintended Use:
These products are not intended for use within the patient environment.
When this manual is translated into other languages, the US English version is binding.
© 2021 Thermo Fisher Scientific. All rights reserved. Printed in U.S.A.

Thermo Scientific Cryobiological Storage Vessel iii
Preface
mportant operating and/or maintenance instructions. Read the accompanying text carefully.
Extreme temperature hazards. Only qualified persons should perform procedures associated with this
symbol.
Potential biological hazards. Proper protective equipment and procedures must be used.
This symbol when used alone indicates important operating instructions which reduce the risk of
injury or poor performance of the unit.
CAUTION: This symbol, in the context of a CAUT ON, indicates a potentially hazardous situation
which if not avoided could result in minor to moderate injury or damage to the equipment. s
CAUTION: This indicates a situation which may result in property damage. s
Before installing, using or maintaining this product, please be sure to read the manual and product
warning labels carefully. Failure to follow these instructions may cause the product to malfunction,
which could result in injury or damage.
WARNING: Unauthorized repair of your freezer will invalidate your warranty. Contact Technical
Service at 1-800-438-4851 for additional information. s

Thermo Scientificiv Cryobiological Storage Vessel
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermofisher.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address
Certified Service Web Pagewww.unitylabservices.com
Our staff can provide information on pricing and give you quotations. We canSales Support
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road, Box 649
Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.

Cryobiological Storage Vessel vThermo Scientific
Table of Contents
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Please Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Measuring Liquid Nitrogen Quantity . . . . . . . . . . . . . . . . . . . . . . . .3-2
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
WEEE Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6

vi Cryobiological Storage Vessel Thermo Scientific
Table of Contents

Cryobiological Storage Vessel 1-1Thermo Scientific
Please Read
Section 1 Safety Information
This manual contains important operating and safety information.
The user must carefully read and understand the contents of this manual
prior to the use of this equipment.
Your Thermo Scientific cryogenic vessel has been designed with function,
reliability, and safety in mind. t is the user’s responsibility to install it in
conformance with local electrical codes. For safe operation, pay attention
to the warnings and cautions throughout the manual.
All cryopreservation vessels, regardless of who manufactures them, use a
vacuum to provide for the super insulative properties needed to keep
nitrogen in a liquid form. They are very similar, in fact, to the thermos
vessels you may have used to store coffee, soup or milk.
Remember how fragile they were? Since no vacuum is perfect or will last
forever, we suggest that you monitor the consumption of liquid nitrogen
used by your vessel on a regular basis, i.e. every 1-2 days. By monitoring,
you may be able to anticipate subtle changes in consumption and possibly
react to problems before they arise. You may opt to use a level monitor for
this purpose.
Also, if your samples are super-critical, one-of-a-kind, or irreplaceable,
consider allocating your samples into 2 separate vessels.
Remember the old adage about placing all your eggs into one basket…
Warnings
• This unit is intended to be used by trained personnel in a
well-ventilated environment.
• Liquid nitrogen is extremely cold; it boils at -196°C.
• Follow all necessary precautions and warnings relevant to applicable
hazards such as frostbite, contact burn, asphyxiation, etc., in cryogenic
areas.

1-2 Cryobiological Storage Vessel Thermo Scientific
Section 1
Safety Information
• To avoid injury due to frostbite or ruptured vials, use extreme care
whenever handling liquid nitrogen, liquid nitrogen storage or transfer
vessels or any objects which have come in contact with liquid
nitrogen.
• Leave no areas of skin exposed.
• Wear adequate PPE where necessary: face shield, cryogenic gloves,
cryogenic apron.
• Only use the power supply provided with the unit.
• Do not tightly seal liquid nitrogen containers or prevent nitrogen gas
from escaping.
• Always handle vials with tongs.
• Use extreme care to prevent spilling and splashing liquid nitrogen
during transfer and removal of storage contents and holders.
• mmediately remove any clothing or safety attire on which liquid
nitrogen has been spilled or splashed .
• Get immediate medical attention for any frostbite injuries due to
liquid nitrogen.
• Never overfill liquid nitrogen vessels.
Use this product only in the way described in the product literature•
and in this manual. Before using it, verify that this product is suitable
for its intended use. f the equipment is used in a manner not
specified by the manufacturer, the protection provided by the
equipment may be impaired.
When inserting or removing cans, be careful not to come in contact
with the neck tube area of the vessel. Remove or insert cans slowly,
in a vertical manner. Scratches on the neck tube can cause remature
vacuum failure.
Do not tam er with, or remove vacuum ort (covered by black
lastic ca on side of vessel); this will void warranty.
Do not spill liquid nitrogen on vacuum port - this can cause vacuum
failure.
Always keep liquid nitrogen in an upright position.

Cryobiological Storage Vessel 2-1Thermo Scientific
Section 2 General Specifications
* Actual working time may vary due to current atmospheric conditions, container
history, manufacturing tolerances, and any individual patterns of use.
Environmental Conditions
Operating temperature: -29°C to +60°C
10% to 95% relative humidity, non-condensing.
Altitude limit: 2,000 meters.
Storage: -29°C to +60°C; 10% to 95% relative humidity.
ntended for indoor use only.
BioCane 20 BioCane 34 BioCane 47 BioCane 73
Height (Inches) 25.70" 26.60" 26.50" 27.00"
Diameter (Inches) 14.50" 18.20" 20.00" 22.00"
L 2 Capacity 20.50 L 34.80 L 47.40 L 73.0 L
Static Holding Time (Days) 227 193 121 121
Static L 2 Evaporation Rate* 0.1 L/day 0.18 L/day 0.40 L/day 0.60 L/day
Liquid Phase Capacity 1.2 ml 180 vials 720 vials 1512 vials 2016 vials