Thermo Scientific TZ-32 Zonal Rotor User manual

Thermo Scientific
TZ-32 Zonal Rotor
for the Thermo Scientific WX+ Centrifuge
Instruction Manual
50146926-a • 01 / 2015

Thermo Scientific TZ-32 Zonal Rotor
— 2
Table of Contents
1. Preface ..............................................4
1. 1. Intended Use ................................................4
1. 2. Items Supplied...............................................5
1. 2. 1. Rotor Assembly ................................................... 5
1. 2. 2. Seal Attachment Assembly ........................................... 7
1. 3. Precautions .................................................9
1. 4. Symbols used in the manual ...................................9
2. Technical Specifications ...............................10
3. Construction .........................................11
3. 1. High-Speed Rotation .........................................11
3. 2. Loading and Unloading .......................................11
4. Assembly ...........................................12
4. 1. Rotor Assembly .............................................12
4. 1. 3. Rotor body and cover .............................................. 12
4. 1. 4. Septa.......................................................... 12
4. 1. 5. Shaft .......................................................... 13
4. 1. 6. Stopper ........................................................ 13
4. 1. 7. Rulon seal ...................................................... 13
4. 1. 8. Cap assembly.................................................... 13
4. 2. RPZ-S Seal Attachment Assembly ..............................14
4. 2. 9. Seal assembly ................................................... 14
4. 2. 10. Guard plate assembly ............................................. 16
4. 2. 11. Shield plate .................................................... 16
5. Operation ...........................................17
5. 1. General Description..........................................20
5. 2. Preparation ................................................21
5. 2. 1. Prepare the Centrifuge ............................................. 21
5. 2. 2. Connect a tube to the seal assembly................................... 25
5. 2. 3. Confirm test operation ............................................. 26
5. 3. Operation ..................................................27
5. 3. 1. Prepare the Rotor ................................................. 27

Thermo Scientific TZ-32 Zonal Rotor
— 3
5. 3. 2. Preparation for spinning the rotor ..................................... 30
5. 3. 3. Spinning........................................................ 31
5. 3. 4. Injection of gradient solution and sample................................ 31
5. 3. 5. High speed spinning ............................................... 35
5. 3. 6. Deceleration and extraction.......................................... 36
5. 4. Operational Checkpoints......................................37
5. 4. 1. Setting of sample temperature and rotor temperature ...................... 37
5. 4. 2. Precautions on loading and unloading .................................. 37
5. 5. Capacity in Rotor............................................39
5. 6. Separation Characteristic .....................................39
5. 7. Rotor Life ..................................................39
6. Maintenance.........................................40
6. 1. Cleaning of Rotor and Septa...................................40
6. 2. Sterilization of Rotor .........................................40
6. 3. Inspection of Rotors .........................................41
6. 4. Handling of Rulon Seal and Renewal............................42
6. 5. Disassembling and Cleaning of Cap Assembly ....................43
6. 6. Disassembling and Cleaning of Seal Assembly ...................43
6. 7. Cleaning of Ball Bearing ......................................44
6. 8. Consumable Parts of the Rotor ................................45
6. 9. Service Parts List for the Cap Assembly .........................45
6. 10. Service Parts List for the Seal Assembly........................46
7. Troubleshooting ......................................47
7. 1. Check the Rulon Seal ........................................48
7. 2. Other Check Points ..........................................49

Thermo Scientific TZ-32 Zonal Rotor
— 4
Table of Figures
Rotor Components......................................................... 8
Seal attachment components (Type RPZ-S. Part cord. 901306) ....................... 10
Construction – High Speed Rotation ........................................... 14
Construction –Loading and Unloading. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Rotor body and cover...................................................... 15
Septa ................................................................. 15
Shaft, Stopper and Rulon Seal ............................................... 16
Cap assembly ........................................................... 16
Seal assembly ........................................................... 17
Construction of the seal assembly ............................................ 18
Guard plate assembly ..................................................... 19
Shield plate............................................................. 19
Overspeed decal View from the bottom......................................... 20
Mounting the supports..................................................... 24
Centering the guard plate .................................................. 25
Loosening the lock nut..................................................... 25
Adjusting the base........................................................ 26
Height adjustment ........................................................ 26
Tightening the three setscrews............................................... 27
Tube connection ......................................................... 28
Setting the seal packing in detail ............................................. 30
Setting the seal packing.................................................... 31
Assembling the Rulon seal and O-ring on the shaft................................ 31
Shaft into septa.......................................................... 32
Assembled septa into rotor ................................................. 32
Assemble the rotor cover ................................................... 33
Mount the guard plate ..................................................... 33
Mount the seal assembly ................................................... 34
Mount the shield plate ..................................................... 35
Feed a gradient solution by pump ............................................ 35
Injection of sample and overlay .............................................. 36
How to avoid bubbles while injecting a density gradient solution and sample ............. 36
Preparation of the pump ................................................... 37
Removal of air bubbles .................................................... 37
Place the cap ........................................................... 38
Checking the cap ........................................................ 38
Deceleration and extraction ................................................. 39
Relation between volume and radius .......................................... 42
Removal of the metal ring .................................................. 43
Rotor inspection points .................................................... 44
Polishing the Rulon seal.................................................... 45
Removal of the Rulon seal .................................................. 45
Parts of the cap assembly .................................................. 46
Construction of the seal assembly ............................................ 47
Service parts list for the cap assembly ......................................... 48
Service parts list for the seal assembly......................................... 49

Thermo Scientific TZ-32 Zonal Rotor
— 5
Table of Tables
Rotor Accessories ......................................................... 8
Seal Attachment Accessories ................................................ 10
Technical Specifications .................................................... 12
Employed materials ....................................................... 20
Chemical Resistance of Noryl................................................ 21
Separation Procedure ..................................................... 22
Operation modes......................................................... 33
Increase of Volume ....................................................... 39
Separation characteristic ................................................... 41
Rotor Life .............................................................. 41
Sterilization methods ...................................................... 43
Troubleshooting .......................................................... 49
Defective phenomena and corrective action sealed sections ......................... 50

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 6
1. Preface
Zonal centrifugation has drawn attention in the biological, medical, and agricultural fields. Swinging
bucket rotors have mainly been used for separating cellular substances, virus, and other fine particles.
The advantages of the zonal rotor over the swinging bucket rotors exist in reduction of convective
disturbance due to “wall effect”, large capacity, and capability of dynamic continuous loading, unloading
and analytical operation of samples while the rotor is spinning. Because of the high performance and
reliability, the TZ-32 Zonal rotor can be used in many fields of laboratory work and commercial production.
1. 1. Intended Use
The rotor is used in combination with the according centrifuge as a laboratory product designed to separate components by
generation of Relative Centrifugal Force (RCF).
If these rotors are used in a manner not specified by the manufacturer, the protection provided by the equipment may be
impaired.
These rotors should be operated by trained specialists only.

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 7
1. 2. Items Supplied
1. 2. 1. Rotor Assembly
Rotor Components
➀ ➁ ➂ ➃
➄ ➅ ➆ ➇
➀Rotor cover
➁Rulon seal (439345)
➂Metal ring (451152)
➃Seal packing (451153)
➄Cap assembly (327251A)
➅Stopper (455897)
➆Shaft (209280)
➇Septa (103547)
CAUTION The TZ-32 zonal rotor assembly does not include the RPZ-S seal attachments assembly. It has to be ordered
seperately. See “Figure 2” on page 7.
Figure 1
Rotor
Components

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 8
Rotor Accessories
Item Article No. Note Quantity Picture
Rulon seal 439345 3
Rotor stand 216715 1
Seal packing 451153 TZ-32 1
O-ring 8062222 1AS22 (JIS-B-2401)
For rotor
2
O-ring 8062014 1AP14 (JIS-B-2401)
For Rulon seal
2
O-ring 8062003 1AP3 (JIS-B-2401)
For Rulon seal
2
O-ring 8062018 1AP18 (JIS-B-2401)
For shaft
6
O-ring 8062218 1AS18 (JIS-B-2401)
For shaft
2
Stopper 455897 2
Instruction manual 50146926 1
Over speed decal 34644414 2
Screwdriver 464912 1
Tool box 320717 1
Circlip remover 84850103 1
Lubricant for screws 84810201 1
Table 1
Rotor Accessories

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 9
1. 2. 2. Seal Attachment Assembly
Seal Attachment Components
➀➁
➂ ➃
➀Part 1 of the shield plate assembly (322897B)
➁Guard plate assembly (104230A)
➂Part 2 of the shield plate assembly (323896B)
➃Seal assembly (217428A)
Figure 2
Seal attachment
components
(Type RPZ-S. Part
cord. 901306)

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 10
Seal Attachment Accessories
Item Article No. Note Quantity Picture
Hexagon socket head
bolt
70017308 M6 x 30 6
D6 spring washer 80112078 D6 6
D6 flat washer 80202088 D6 6
Tee connector 484286 4
Rubber tube clamp 466544 6
Tygon tube 662905 R-3603
1/8” x 1/4” x 5 m
1
Ball bearing 60000178 1
Support S408766 3
Spacer S408765 3
Extractor 479965 For Rulon seal 1
Seal rubber S202661 1
Snap ring remover 84850109 1
Hexagon bar wrench 60000122 For M4 setscrew 1
Holder base 343920A 1
Handle 323663A 1
Table 2
Seal Attachment
Accessories

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 11
1. 3. Precautions
• Do not spin a rotor beyond the allowable speed.
»The maximum speed marked on a rotor refers to an allowable one when the maximum density of the sample
or density gradient solution is less than 1.2.
»When a sample or density gradient solution of more than 1.2 in maximum density is used, the maximum
speed must be reduced according to the instruction manual for the rotor.
»Upon reaching the end of the service life of a rotor specified in its instruction manual, the allowable speed
should be reduced by 10%.
• Set a rotor gently and securely onto the drive shaft hub.
»Practice setting repeatedly to acquire the knack.
• Confirm that the rotor is free from corrosion and scores.
»If white corrosion is recognized on a tube hole bottom of an angle rotor, or a hole bottom of a bucket for
swing rotor, immediately stop using it and contact the nearest agent of Thermo Fisher Scientific
»When operation is over, store the rotor after cleaning and drying.
• Make it a practice to keep a logbook for rotors.
»Keeping a logbook for rotors is indispensable for controlling the life or when the warranty clause is involved.
Keep the logbook up to date and store it properly.
• Do not rotate this rotor at a speed of more than 5000 rpm unless all components (rotor assembly, cap assembly)
are assembled.
Do not rotate this rotor at a speed of more than 5000 rpm unless the rotor is filled with liquid.
If the above cautions are not observed, septa could be damaged.
• Always ground the ultracentrifuge before use.
1. 4. Symbols used in the manual
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Obey the safety information contained in the instruction manual to keep you and your
environment safe.

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 12
2. Technical Specifications
Maximum Speed 32000 rpm
Maximum Centrifugal Force 102000 x g
K-factor 363
Capacity 1690 ml
Maximum Density of Gradient Solution 1.2 g/ml
Maximum Radius 8.89 cm
Rotor Material Titanium Alloy
Weight 15.4 kg
Table 3
Technical
Specifications

Thermo Scientific TZ-32 Zonal Rotor
High-Speed Rotation — 13
3. Construction
3. 1. High-Speed Rotation
➀
➁
➀Cap
➁Cover of rotor
3. 2. Loading and Unloading








➀Pipe for cooling water
➁Pipe in the rotor center
➂Pipe on the outer wall of rotor
➃Seal assembly
➄Guard plate
➅Shaft
➆Rotor
➇Over-speed decal
➈Septa
Figure 3
Construction –
High Speed
Rotation
Figure 4
Construction –
Loading and
Unloading

Thermo Scientific TZ-32 Zonal Rotor
Rotor Assembly — 14
4. Assembly
4. 1. Rotor Assembly
The rotor is composed of a rotor body, cover, septa, shaft, cap assembly and Rulon™seal attached to the shaft. The TZ-32
rotor has a capacity of 1690 ml.
4. 1. 3. Rotor body and cover
The rotor body and cover of the TZ-32 rotor use, as material, titanium alloy of an excellent corrosion resistance. The seal
packing is fluorine rubber. The metal ring is titanium alloy.
➀
➁
➂
➀Cap assembly
➁Cover
➂Rotor body
4. 1. 4. Septa
The septa has four vanes that divide the bowl into four sector-shaped compartments, each vane containing a channel to
carry fluid to the outer edge of the rotor bowl. The solution injected through the seal assembly is led to the outer edge of
the rotor from the four holes located outside the Rulon seal through the shaft. The septa has slanting surfaces, the top of
each being provided with a hole communicating from the center of the seal assembly with the hole in the center of the
Rulon seal. Otherwise, the septa is made of a plastic (Noryl) for which the chemical resistance, cautions in spinning, and
the maintenance will be described later.
Figure 5
Rotor body and
cover
Figure 6
Septa

Thermo Scientific TZ-32 Zonal Rotor
Rotor Assembly — 15
4. 1. 5. Shaft
A shaft is inserted in the central part of septa, and form a sample passage way. And, the tops of the shaft become the
installation of the cap and the connection part of seal assembly. The material of the shaft uses titanium alloy.
4. 1. 6. Stopper
A stopper is the coming off stop part when a cap is installed, and material is Noryl.
4. 1. 7. Rulon seal
The Rulon seal mouted on the top of shaft. This part is important component in loading and unloading, and material is
fluorine plastic.
➀
➁
➂
4. 1. 8. Cap assembly
The cap is mounted onto the shaft with the seal assembly removed after injecting a sample into the rotor. It seals the
sample in the rotor when evacuating the rotor chamber and spinning the rotor at a high speed.
The cap can be easily and securely removed by pushing the extruded portion (push button) atop the cap.
Figure 7
Shaft, Stopper and
Rulon Seal
Figure 8
Cap assembly

Thermo Scientific TZ-32 Zonal Rotor
Rotor Assembly — 16
4. 2. RPZ-S Seal Attachment Assembly
4. 2. 9. Seal assembly
The seal assembly is mounted onto the rotor shaft as shown in Figure 10. The rotor, shaft, and adapter rotate, but the other
components do not rotate. The outer side of the holder cover is supported by the guard plate which will be described later.
The solution injected through the pipe is introduced through the body and Rulon seal into the rotor being spinned. One of
the two higher pipes projecting from the body, which is located on the outer wall side of the rotor, is led to the outer groove
of the Rulon seal and the outer wall of the rotor from the four septa vanes through the four holes of the Rulon seal. The
pipe in the rotor center is led to the four holes on top of the septa through the center of the shaft. The two lower pipes are
used for circulating cooling water into the interior of the body.
Figure 9
Seal assembly

Thermo Scientific TZ-32 Zonal Rotor
Rotor Assembly — 17
➀
➁
➂
➃
➄
➅
➆
➇
➈
➉
11
12
13
14
15
➀Body cooling water pipes
➁Pipe in the rotor center
➂Cooling water pipe
➃Coil spring (B)
➄Body
➅Seal Assembly
➆Ball bearing
➇Adaptor
➈Cover
➉Shaft
11
Rotor body
11
Rulon seal
12
Holder cover
13
Guide body
14
Pipe
15
Pipe on the outer wall side of rotor
Figure 10
Construction of the
seal assembly

Thermo Scientific TZ-32 Zonal Rotor
Rotor Assembly — 18
4. 2. 10. Guard plate assembly
The center case supports the seal assembly and maintains the contact between the body and the Rulon seal for injecting
solution into and extracting it, from the spinning rotor. It is mounted by using the three supports leading from the rotor
chamber of the centrifuge. It is made of a transparent acryl resin plate for the spinning rotor to be visible. It also prevents
tools, etc. from dropping into the rotor chamber while it is spinning.
➀
➀Case
4. 2. 11. Shield plate
It is mounted on top of the rotor chamber to suppress frosting in the chamber when injecting gradient solution or samples
into or extracting them from the rotor which is being cooled.
Since the shield plate is also made of a transparent acrylic resin plate, the rotor chamber is clearly visible through this
plate.
A tube setter is provided for retaining the tubes projecting from the seal assembly. Since the shield plate is divided into
two semicircular portions, it is easily mountable or removable.
➀
➀Tube setter
Figure 11
Guard plate
assembly
Figure 12
Shield plate

Thermo Scientific TZ-32 Zonal Rotor
Rotor Assembly — 19
5. Operation
CAUTION
Never spin the rotor at a speed over 5,000 rpm unless it is filled with the liquid.
If this is neglected, the septa will be damaged.
CAUTION
The over-speed decal, if corroded or discolored must be replaced immediately.
The over speed decal is important to control the rotor revolution speed.
The over-speed decal must match the maximum speed of the rotor.
When replacing be very careful not to damage the over-speed decal and rotor body.
Replacing over-speed decal
To replace the over-speed decal follow this procedure:
1. Preparation
»Prepare a new over-speed decal and knife.
»Wash and then dry the rotor well.
2. Hold the rotor upside down.
3. Pry the edge of the over-speed decal with the knife and remove the decal.
Be very careful not to damage the rotor.
4. Clean the decal hole on the rotor by using alcohol.
5. Remove the backing paper from the new over-speed decal.
Place the over-speed decal so that it fits into the groove of the decal hole on the rotor.
Make sure the decal does not move.
➀
➁
Part No. 34644414
➀Over-speed decal
➁Rotor
Figure 13
Overspeed decal
View from the
bottom

Thermo Scientific TZ-32 Zonal Rotor
Rotor Assembly — 20
Maximum Speed
The maximum speed of the rotor inscribed on the rotor cover surface is determined using the maximum density of gradient
solution below 1.2 g/ml.
If the maximum denstity of gradient solution exceeds 1.2 g/ml, decrease the maximum speed of the rotor according to
the following equation:
Employed Materials
The following materials are used on the passage of the sample:
Part name Material
Tygon tube PVC (specially processed)
Tee Polypropylene
Metallic section of seal attachment assembly etc. Stainless steel
Stationary seal surface (seal assembly side) Tungsten carbide
Rulon seal Tetrafluorethylene resin (Rulon)
O-Ring NBR
Rotor, cover, shaft, metal ring Titanium alloy
Septa Noryl (polyphenylene oxide resin)
Seal packing Fluorine rubber
Table 4
Employed materials