Thermo Scientific TZ-32 Zonal Rotor User manual

Thermo Scientific
TZ-32 Zonal Rotor
for the Thermo Scientific WX+ Centrifuge
Instruction Manual
50146926-a • 01 / 2015

Thermo Scientific TZ-32 Zonal Rotor
— 2
Table of Contents
1. Preface ..............................................4
1. 1. Intended Use ................................................4
1. 2. Items Supplied...............................................5
1. 2. 1. Rotor Assembly ................................................... 5
1. 2. 2. Seal Attachment Assembly ........................................... 7
1. 3. Precautions .................................................9
1. 4. Symbols used in the manual ...................................9
2. Technical Specifications ...............................10
3. Construction .........................................11
3. 1. High-Speed Rotation .........................................11
3. 2. Loading and Unloading .......................................11
4. Assembly ...........................................12
4. 1. Rotor Assembly .............................................12
4. 1. 3. Rotor body and cover .............................................. 12
4. 1. 4. Septa.......................................................... 12
4. 1. 5. Shaft .......................................................... 13
4. 1. 6. Stopper ........................................................ 13
4. 1. 7. Rulon seal ...................................................... 13
4. 1. 8. Cap assembly.................................................... 13
4. 2. RPZ-S Seal Attachment Assembly ..............................14
4. 2. 9. Seal assembly ................................................... 14
4. 2. 10. Guard plate assembly ............................................. 16
4. 2. 11. Shield plate .................................................... 16
5. Operation ...........................................17
5. 1. General Description..........................................20
5. 2. Preparation ................................................21
5. 2. 1. Prepare the Centrifuge ............................................. 21
5. 2. 2. Connect a tube to the seal assembly................................... 25
5. 2. 3. Confirm test operation ............................................. 26
5. 3. Operation ..................................................27
5. 3. 1. Prepare the Rotor ................................................. 27

Thermo Scientific TZ-32 Zonal Rotor
— 3
5. 3. 2. Preparation for spinning the rotor ..................................... 30
5. 3. 3. Spinning........................................................ 31
5. 3. 4. Injection of gradient solution and sample................................ 31
5. 3. 5. High speed spinning ............................................... 35
5. 3. 6. Deceleration and extraction.......................................... 36
5. 4. Operational Checkpoints......................................37
5. 4. 1. Setting of sample temperature and rotor temperature ...................... 37
5. 4. 2. Precautions on loading and unloading .................................. 37
5. 5. Capacity in Rotor............................................39
5. 6. Separation Characteristic .....................................39
5. 7. Rotor Life ..................................................39
6. Maintenance.........................................40
6. 1. Cleaning of Rotor and Septa...................................40
6. 2. Sterilization of Rotor .........................................40
6. 3. Inspection of Rotors .........................................41
6. 4. Handling of Rulon Seal and Renewal............................42
6. 5. Disassembling and Cleaning of Cap Assembly ....................43
6. 6. Disassembling and Cleaning of Seal Assembly ...................43
6. 7. Cleaning of Ball Bearing ......................................44
6. 8. Consumable Parts of the Rotor ................................45
6. 9. Service Parts List for the Cap Assembly .........................45
6. 10. Service Parts List for the Seal Assembly........................46
7. Troubleshooting ......................................47
7. 1. Check the Rulon Seal ........................................48
7. 2. Other Check Points ..........................................49

Thermo Scientific TZ-32 Zonal Rotor
— 4
Table of Figures
Rotor Components......................................................... 8
Seal attachment components (Type RPZ-S. Part cord. 901306) ....................... 10
Construction – High Speed Rotation ........................................... 14
Construction –Loading and Unloading. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Rotor body and cover...................................................... 15
Septa ................................................................. 15
Shaft, Stopper and Rulon Seal ............................................... 16
Cap assembly ........................................................... 16
Seal assembly ........................................................... 17
Construction of the seal assembly ............................................ 18
Guard plate assembly ..................................................... 19
Shield plate............................................................. 19
Overspeed decal View from the bottom......................................... 20
Mounting the supports..................................................... 24
Centering the guard plate .................................................. 25
Loosening the lock nut..................................................... 25
Adjusting the base........................................................ 26
Height adjustment ........................................................ 26
Tightening the three setscrews............................................... 27
Tube connection ......................................................... 28
Setting the seal packing in detail ............................................. 30
Setting the seal packing.................................................... 31
Assembling the Rulon seal and O-ring on the shaft................................ 31
Shaft into septa.......................................................... 32
Assembled septa into rotor ................................................. 32
Assemble the rotor cover ................................................... 33
Mount the guard plate ..................................................... 33
Mount the seal assembly ................................................... 34
Mount the shield plate ..................................................... 35
Feed a gradient solution by pump ............................................ 35
Injection of sample and overlay .............................................. 36
How to avoid bubbles while injecting a density gradient solution and sample ............. 36
Preparation of the pump ................................................... 37
Removal of air bubbles .................................................... 37
Place the cap ........................................................... 38
Checking the cap ........................................................ 38
Deceleration and extraction ................................................. 39
Relation between volume and radius .......................................... 42
Removal of the metal ring .................................................. 43
Rotor inspection points .................................................... 44
Polishing the Rulon seal.................................................... 45
Removal of the Rulon seal .................................................. 45
Parts of the cap assembly .................................................. 46
Construction of the seal assembly ............................................ 47
Service parts list for the cap assembly ......................................... 48
Service parts list for the seal assembly......................................... 49

Thermo Scientific TZ-32 Zonal Rotor
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Table of Tables
Rotor Accessories ......................................................... 8
Seal Attachment Accessories ................................................ 10
Technical Specifications .................................................... 12
Employed materials ....................................................... 20
Chemical Resistance of Noryl................................................ 21
Separation Procedure ..................................................... 22
Operation modes......................................................... 33
Increase of Volume ....................................................... 39
Separation characteristic ................................................... 41
Rotor Life .............................................................. 41
Sterilization methods ...................................................... 43
Troubleshooting .......................................................... 49
Defective phenomena and corrective action sealed sections ......................... 50

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 6
1. Preface
Zonal centrifugation has drawn attention in the biological, medical, and agricultural fields. Swinging
bucket rotors have mainly been used for separating cellular substances, virus, and other fine particles.
The advantages of the zonal rotor over the swinging bucket rotors exist in reduction of convective
disturbance due to “wall effect”, large capacity, and capability of dynamic continuous loading, unloading
and analytical operation of samples while the rotor is spinning. Because of the high performance and
reliability, the TZ-32 Zonal rotor can be used in many fields of laboratory work and commercial production.
1. 1. Intended Use
The rotor is used in combination with the according centrifuge as a laboratory product designed to separate components by
generation of Relative Centrifugal Force (RCF).
If these rotors are used in a manner not specified by the manufacturer, the protection provided by the equipment may be
impaired.
These rotors should be operated by trained specialists only.

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 7
1. 2. Items Supplied
1. 2. 1. Rotor Assembly
Rotor Components
➀ ➁ ➂ ➃
➄ ➅ ➆ ➇
➀Rotor cover
➁Rulon seal (439345)
➂Metal ring (451152)
➃Seal packing (451153)
➄Cap assembly (327251A)
➅Stopper (455897)
➆Shaft (209280)
➇Septa (103547)
CAUTION The TZ-32 zonal rotor assembly does not include the RPZ-S seal attachments assembly. It has to be ordered
seperately. See “Figure 2” on page 7.
Figure 1
Rotor
Components

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 8
Rotor Accessories
Item Article No. Note Quantity Picture
Rulon seal 439345 3
Rotor stand 216715 1
Seal packing 451153 TZ-32 1
O-ring 8062222 1AS22 (JIS-B-2401)
For rotor
2
O-ring 8062014 1AP14 (JIS-B-2401)
For Rulon seal
2
O-ring 8062003 1AP3 (JIS-B-2401)
For Rulon seal
2
O-ring 8062018 1AP18 (JIS-B-2401)
For shaft
6
O-ring 8062218 1AS18 (JIS-B-2401)
For shaft
2
Stopper 455897 2
Instruction manual 50146926 1
Over speed decal 34644414 2
Screwdriver 464912 1
Tool box 320717 1
Circlip remover 84850103 1
Lubricant for screws 84810201 1
Table 1
Rotor Accessories

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 9
1. 2. 2. Seal Attachment Assembly
Seal Attachment Components
➀➁
➂ ➃
➀Part 1 of the shield plate assembly (322897B)
➁Guard plate assembly (104230A)
➂Part 2 of the shield plate assembly (323896B)
➃Seal assembly (217428A)
Figure 2
Seal attachment
components
(Type RPZ-S. Part
cord. 901306)

Thermo Scientific TZ-32 Zonal Rotor
Intended Use — 10
Seal Attachment Accessories
Item Article No. Note Quantity Picture
Hexagon socket head
bolt
70017308 M6 x 30 6
D6 spring washer 80112078 D6 6
D6 flat washer 80202088 D6 6
Tee connector 484286 4
Rubber tube clamp 466544 6
Tygon tube 662905 R-3603
1/8” x 1/4” x 5 m
1
Ball bearing 60000178 1
Support S408766 3
Spacer S408765 3
Extractor 479965 For Rulon seal 1
Seal rubber S202661 1
Snap ring remover 84850109 1
Hexagon bar wrench 60000122 For M4 setscrew 1
Holder base 343920A 1
Handle 323663A 1
Table 2
Seal Attachment
Accessories