Thermo Scientific Finnpipette F1 User manual

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Finnpipette®F1
Single Channel
Variable & Fixed Volume
Multichannel
Instructions for Use
Bedienungsanleitung
Guide d´utilisation
Instrucciones de uso
取扱説明書
User Manual

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This product complies with the European Union Directive 98/79/EC, and it is marked with
a CE-marking.
When the product is used in applications related to the directive 98/79/EC, read the
additional information at www.thermofisher.com or contact the manufacturer at info.
pipettes@thermofisher.com to ensure correct and safe use.
The CE-mark covers the system containing the CE-marked Finnpipette F1 pipette and the
CE-marked Finntips.
Product specifications are subject to change without prior notice. Finnpipette®and Finntip®
are registered trademarks of Thermo Fisher Scientific Oy.
Ce produit est conforme à la directive de l’Union européenne 98/79/CE et porte le
marquage CE.
Lorsque le produit est utilisé pour des applications relatives à la directive 98/79/EC, veuillez
prendre connaissance des informations complémentaires à www.thermofisher.com ou
contacter le constructeur à info.pipettes@thermofisher.com. Ceci pour garantir un usage
adéquat et sûr.
Le marquage CE s’applique au système comprenant la Finnpipette F1 et les cônes Finntip.
Les spécifications du produit sont sujettes à modification sans avis préalable. Finnpipette®
et Finntip®sont des marques déposées de Thermo Fisher Scientific Oy.
Este producto cumple la Directiva de la Unión Europea 98/79/CE y presenta el marcado CE.
Cuando el producto sea usado en aplicaciones afectadas por la directiva 98/79/EC, por favor,
lea la información adicional en www.thermofisher.com o contacte con el fabricante en
info.pipettes@thermofisher.com para asegurar su uso correcto y seguro.
El marcado CE corresponde al sistema que contiene la pipeta Finnpipette F1 con el marcado
CE y las puntas de pipeta Finntip con marcado CE.
Las especificaciones del producto pueden cambiar sin previo aviso. Finnpipette®y Finntip®
son marcas registradas de Thermo Fisher Scientific Oy.
本製品は欧州指令98/79/ECに準拠しており、CEマーキングが付いています。
製品を指令98/79/ECに関連するアプリケーションで使用するときは、適切で安全に
ご使用いただくため、www.thermofisher.com に掲載の追加情報をお読みになる
か、製造元[e-mail] にお問い合わせください。
CEマークは、CEマークの付いた Finnpipette F1ピペットおよびCEマークの付いた
Finntipピペットチップを含むシステムを対象としています。
製品仕様は事前の予告なく変更されることがあります。フィンピペット®とフィンチ
ップ®は、サーモフィッシャーサイエンティフィックの登録商標です。
Dieses Produkt entspricht der Europäischen Richtlinie 98/79/EG und ist mit dem CE-Symbol
gekennzeichnet.
Sollte das Produkt in Anwendungen verwendet werden, die in Verbindung zur Direktive
98/79/EC stehen, lesen Sie die Zusatzinformationen auf www.thermofisher.com oder
kontaktieren Sie den Hersteller unter info.pipettes@thermofisher.com, um die richtige
und sichere Verwendung zu gewährleisten.
Die CE-Kennzeichnung bezieht sich auf die Kombination der Finnpipette F1 mit Finntip-
Pipettenspitzen.
Wir behalten uns das Recht auf unangekündigte Änderungen der Produktspezifikationen vor.
Finnpipette®und Finntip®sind eingetragene Warenzeichen der Fa. Thermo Fisher Scientific Oy.

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CONTENTS
PRODUCT DESCRIPTION 4
PACKAGE 5
SAFETY 5
PIPETTE OPERATION 6
PIPETTING TECHNIQUES 7
CALIBRATION AND ADJUSTMENT 8
MAINTENANCE 12
TROUBLE SHOOTING 14
SPARE PARTS 63-71
English
INHALT
PRODUKTBESCHREIBUNG 15
PACKUNGSINHALT 16
SICHERHEIT 16
BEDIENUNG DER PIPETTE 17
PIPETTIERMETHODEN 18
KALIBRIERUNG UND JUSTIERUNG 19
WARTUNG 23
FEHLERBEHEBUNG 26
ERSATZTEILE UND ZUBEHÖR 63-71
Deutsch
SOMMAIRE
DESCRIPTION DU PRODUIT 27
CONDITIONNEMENT 28
CONSIGNES DE SÉCURITÉ 28
UTILISATION DE LA PIPETTE 29
MÉTHODES DE PIPETAGE 30
CALIBRAGE 31
ENTRETIEN 35
EN CAS DE PROBLEME 37
PIÈCES DÉTACHÉES 63-71
Français
CONTENIDO
DESCRIPCIÓN DEL PRODUCTO 38
PAQUETE 38
SEGURIDAD 38
USO DE LA PIPETA 40
TÉCNICAS DE PIPETEO 41
CALIBRACIÓN Y AJUSTE 42
MANTENIMIENTO 46
SOLUCIÓN DE PROBLEMAS 49
PIEZAS DE RECAMBIO 63-71
Español
目次
製品について 50
パッケージ 51
安全にお使いいただくために 51
ピペットの操作 52
ピペッティングテクニック 53
キャリブレーション 54
メンテナンス 58
トラブルシューティング 60
保証規定 62
パーツ及び付属品 63-71
日
本
語

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Product description
The Finnpipette F1 is a continuously adjustable, general purpose micropipette for sampling and
dispensing accurate liquid volumes.
It operates on an air displacement principle (i.e. an air interface) and uses detachable, disposable tips.
The adjusted delivery volume is displayed digitally on a readout window in the handle.
The fourteen different models of Finnpipette F1 pipettes cover a volume range from 0,2 μl to 10 ml.
Order No. Volume Range Color code Finntip
4641010N 0,2 μl to 2 μl pink Flex 10, 10, 20, 50
4641020N 0,5 μl to 5 μl pink Flex 10, 10, 20, 50
4641030N 1 μl to 10 μl pink Flex 10, 10, 20, 50
4641040N 1 μl to 10 μl yellow
Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4641050N 2 μl to 20 μl turquoise 50
4641060N 2 μl to 20 μl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4641130N 5 μl to 50 μl turquoise 50
4641140N 5 μl to 50 μl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4641070N 10 μl to 100 μl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4641080N 20 μl to 200 μl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4641090N 30 μl to 300 μl orange Flex 300, 300
4641100N 100 μl to 1000 μl blue Flex 1000, 1000, 1000 Ext
4641110N 0,5 ml to 5 ml green 5 ml
4641120N 1 ml to 10 ml red 10 ml, Flex 10 ml Ext
The thirteen different models of Finnpipette F1 Fixed Volume pipettes cover a volume range
from 1 μl to 10 ml.
Order No. Volume Range Color code Finntip
4651020N 10 μl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4651130N 20 μl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4651030N 25 μl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4651040N 50 μl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4651050N 100 μl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4651140N 200 μl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4651060N 250 μl dark blue Flex 1000, 1000, 1000 Ext
4651070N 500 μl dark blue Flex 1000, 1000, 1000 Ext
4651080N 1000 μl dark blue Flex 1000, 1000, 1000 Ext
4651090N 2000 μl dark blue 5 ml
4651100N 3000 μl dark blue 5 ml
4651110N 5000 μl dark blue 5 ml
4651120N 10000 μl dark blue 10 ml, Flex 10 ml Ext
The ten different models of Finnpipette F1 Multichannel pipettes cover a volume range from
1 μl to 300 μl.
Order No. Channel Volume Range Color code Finntip
4661000N 8 1 μl to 10 μl pink Flex 10, 10, 20, 50
4661010N 8 5 μl to 50 μl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4661020N 8 10 μl to 100 μl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4661030N 8 30 μl to 300 μl orange Flex 300, 300
4661040N 12 1 μl to 10 μl pink Flex 10, 10, 20, 50
4661050N 12 5 μl to 50 μl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4661060N 12 10 μl to 100 μl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4661070N 12 30 μl to 300 μl orange Flex 300, 300
4661080N 16 1 μl to 10 μl purple 20
4661090N 16 5 μl to 50 μl turquoise 50
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English
Digital display
The adjusted delivery volume is clearly indicated in the large
digital display on the handle.
Raw materials
The Finnpipette F1 is made of mechanically durable and chemically resistant materials.
For waste disposal instructions, please contact your local environmental agency. For more
information, please contact us at info.pipettes@thermofisher.com.
Description of tips
Finntips are recommended for use with the Finnpipette F1.
They are made of virgin natural colour polypropylene, generally regarded as the only
contamination free material suitable for tips. Finntips are also autoclavable (121°C).
Package
The Finnpipette F1 is shipped in a specially designed package containing the following items:
1. The Finnpipette
2. Service tool
3. Multichannel service tool
4. Finntip sample
5. Tube of grease (Order No. 2203130)
6. Instruction manual
7. Calibration certificate / Warranty certificate
8. Shelf hanger (Order No. 2206040)
9. Two stickers
Safety
Cautions are marked with this symbol .
Intended use
The intended use of the device is to transfer liquids in the volume range of 0,2 μl to 10 ml.
The F1 Finnpipette pipette and Finntips are designed as a component of an analyzing system
for an end user, who is responsible for validating the system to ensure reliable and safe results.
Damage to health
Follow general procedures for hazard prevention and safety instructions; e.g. wear
protective clothing, eye protection and gloves.
For use and waste disposal of hazardous (e.g. radioactive and potentially infectious)
material, follow the safety instructions and general laboratory practice.
The pipette and tips are not intended for in vivo use. Do not use the pipette for pipetting any
liquid to be injected into a human body.
Do not eject the tip towards anybody.
The Finnpipette F1 is to be used by trained personnel with required laboratory skills. The
instructions for use must be read prior to and during the use of the device (pipette and tip). The
F1 pipette can be used between +4°C and +40°C.
Incorrect dispensing results
Performance may vary due to:
a. pipetting method (forward pipetting technique recommended)
b. temperature (air, liquid, vessel, pipette, and tip)
c. pressure
d. humidity
e. operator, e.g. thumb movement, pipetting angle
f. liquid density, viscosity and vapor pressure
g. type of tip

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If the pipetting performance is critical to the outcome of a specific application, the result has
to be assured with an alternative test, and if this is not an option, by duplicate testing.
The possibility of an incorrect volume delivery during pipetting cannot be entirely mitigated.
To avoid inaccurate dispensing and/or leakage, check that the tip is properly attached to
the pipette.
When rotating the volume adjustment button, do not exceed the volume range of the pipette.
Otherwise the pipette may be damaged or affect pipetting performance.
Pipetting performance cannot be guaranteed if the tip is reused.
Choose only a tip and pipette with matching color coding.
Disposal of the Materials
Follow laboratory and country-specific procedures for the disposal of biohazardous or radioactive
waste. Refer to local regulations for the disposal of infectious material.
Warning The tips can be potentially infectious. Dispose of all used disposable tips as
biohazardous waste.
Warning Decontaminate the pipette before disposal.
For waste disposal instructions, contact your local environmental agency. For more information,
please contact us at info.pipettes@thermofisher.com
Regarding the original packaging and packing materials, use the recycling operators known
to you.
For more information, contact your local Thermo Fisher Scientific representative.
Pipette operation
Setting the delivery volume
1. Set the delivery volume using the knob on the top of the pipette.
Pull the knob to activate the volume setting.
2. To increase the delivery volume, turn the knob
counterclockwise. To decrease the delivery volume,
turn it clockwise.
3. Make sure that the desired delivery volume clicks into place.
4. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the knob outside the range may
jam the mechanism and eventually damage the pipette.
5. Lock the volume by pushing the knob down.
ID-tag
You can mark the pipette application, your initials, the
calibration date, etc. on the ID-tag. Remove the pipette’s
module to replace the tag. Mark the label with a pencil and
slide the tag together with the holder back to it’s slot.
Tip ejection
To help eliminate the risk of contamination, each pipette is
fitted with a tip ejector system. To release the tip, point the
pipette at suitable waste receptacle and press the tip ejector
with your thumb.
Adjusting the finger rest
The finger rest can be adjusted by rotating it 60 degrees
to both directions of the center position. Usually right
handed operators turn it left (counter clockwise) to get the
best possible position for the thumb to eject the tip. See
picture.
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Pipetting techniques
Push and release the knob slowly at all times particularly when working with high viscosity
liquids. Never allow the knob to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with the solution
that you will be pipetting. Hold the pipette in an upright position while aspirating liquid. The
finger rest should rest on your index finger. Make sure that the tips, pipette and solution are at
the same temperature.
Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the knob to the first stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm and slowly
release the knob. Withdraw the tip from the
liquid touching it against the edge of the reservoir
to remove excess liquid.
3. Deliver the liquid by gently depressing the knob
to the first stop. After a delay of about one
second, continue to depress the knob all the way
to the second stop. This action will empty the tip.
4. Release the knob to the ready position. If necessary, change the tip and continue pipetting.
Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.
1. Depress the knob all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the knob. This action will fill the tip.
Withdraw the tip from the liquid touching it
against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the knob to the first stop. Hold the knob at the first
stop. Some liquid will remain in the tip and this should not be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into
the container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the knob all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly re
lease the knob. This action will fill the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing
the knob to the first stop. Hold the knob at the
first stop. Some liquid will remain in the tip and
this should not be included in the delivery.
4. Continue pipetting by repeating steps 3 and 4.
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Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the
knob to the first stop, making sure the tip is well
below the surface.
2. Release the knob slowly to the ready position.
This will fill the tip. Keep the tip in the solution.
3. Depress the knob to the first stop and release
slowly. Keep repeating this procedure until the
interior wall of the tip is clear.
4. Finally, depress the knob all the way to the
second stop to completely empty the tip.
Calibration and adjustment
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water using the forward pipetting technique. It should be noted that the
use of other pipetting techniques may affect the calibration results. The pipettes are constructed
to permit re-adjustment for other pipetting techniques or liquids of different temperature and
viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:
Volume range readable graduation
under 10 μl 0.00 1 mg
10-100 μl 0.01 mg
above 100 μl 0.1 mg
Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C
to 30°C. The relative humidity must be above 50%. Especially with volumes under 50 μl the
air humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
Procedure to check calibration
The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Use of
forward pipetting technique is recommended. The maximum permissible errors are designed for
forward method. It is recommended to calibrate all channels of the multichannel pipette, but a
suitable method is also to calibrate only the edge channels. Adjustment check prior calibration is
done using one of the middle channels. It is recommended to use the ISO8655 calibration limits
presented in the following tables or user-defined specifications.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
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ISO8655 calibration specifications
Thermo Scientific Finnpipette F1 specifications according to ISO8655 standard.
Finnpipette F1 Variable Volume Single Channel
Range Volume Inaccuracy Imprecision
μl μl % s.d. μl cv%
0,2-2 μl 2 ±0.080 ±4 0.040 2.0
0.2 ±0.080 ±40 0.040 20.0
0,5-5 μl 5 ±0.125 ±2.5 0.075 1.5
0.5 ±0.125 ±25 0.075 15
1-10 μl 10 ±0.120 ±1.2 0.080 0.8
1 ±0.120 ±12 0.080 8.0
2-20 μl 20 ±0.20 ±1.0 0.10 0.5
2 ±0.20 ±10.0 0.10 5.0
5-50 μl 50 ±0.50 ±1.0 0.20 0.4
5 ±0.50 ±10 0.20 4.0
10-100 μl 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
20-200 μl 200 ±1.60 ±0.8 0.60 0.3
20 ±1.60 ±8.0 0.60 3.0
30-300 μl 300 ±4.0 ±1.3 1.5 0.5
30 ±4.0 ±13 1.5 5.0
100-1000 μl 1000 ±8.0 ±0.8 3.0 0.3
100 ±8.0 ±8.0 3.0 3.0
0,5-5 ml 5000 ±40.0 ±0.8 15.0 0.3
500 ±40.0 ±8.0 15.0 3.0
1-10 ml 10000 ±60.0 ±0.6 30.0 0.3
1000 ±60.0 ±6.0 30.0 3.0
Finnpipette F1 Fixed Volume Single Channel
Fixed Volume Inaccuracy Imprecision
μl μl % s.d.μl cv%
10 ±0.120 ±1.20 0.080 0.80
20 ±0.20 ±1.00 0.10 0.50
25 ±0.50 ±2.00 0.20 0.80
50 ±0.50 ±1.00 0.20 0.40
100 ±0.80 ±0.80 0.30 0.30
200 ±1.60 ±0.80 0.60 0.30
250 ±4.00 ±1.60 1.50 0.60
500 ±4.00 ±0.80 1.50 0.30
1000 ±8.00 ±0.80 3.00 0.30
2000 ±16.0 ±0.80 6.00 0.30
3000 ±40.0 ±1.33 15.00 0.50
5000 ±40.0 ±0.80 15.00 0.30
10000 ±60.0 ±0.60 30.00 0.30
Finnpipette F1 Variable Volume Multichannel
Range Channel Volume Inaccuracy Imprecision
μl μl % s.d.μl cv%
1-10 μl 8, 12, 16 10 ±0.24 ±2.4 0.16 1.6
1 ±0.24 ±24 0.16 16
5-50 μl 8, 12, 16 50 ±1.0 ±2.0 0.4 0.8
5 ±1.0 ±20 0.4 8.0
10-100 μl 8, 12 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
30-300 μl 8, 12 300 ±8.0 ±2.7 3.0 1.0
30 ±8.0 ±26.7 3.0 10.0
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Manufacturer specification limits
Finnpipette F1 Variable Volume Single Channel
Range Volume Inaccuracy Imprecision Factory
μl μl % s.d. μl cv% calibration tip
0.2-2 μl 2 ±0,050 ±2,50 0,040 2,00 Flex 10
0,2 ±0,024 ±12,00 0,020 10,00
0.5-5 μl 5 ±0,075 ±1,50 0,050 1,00 Flex 10
0,5 ±0,030 ±6,00 0,025 5,00
1-10 μl, micro 10 ±0,100 ±1,00 0,050 0,50 Flex 10
1 ±0,025 ±2,50 0,020 2,00
1-10 μl 10 ±0,100 ±1,00 0,080 0,80 Flex 200
1 ±0,035 ±3,50 0,030 3,00
2-20 μl, micro 20 ±0,20 ±1,00 0,08 0,40 50
2 ±0,06 ±3,00 0,05 2,50
2-20 μl 20 ±0,20 ±1,00 0,08 0,40 Flex 200
2 ±0,06 ±3,00 0,05 2,50
5-50 μl, micro 50 ±0,30 ±0,60 0,15 0,30 50
5 ±0,15 ±3,00 0,125 2,50
5-50 μl 50 ±0,30 ±0,60 0,15 0,30 Flex 200
5 ±0,15 ±3,00 0,125 2,50
10-100 μl 100 ±0,80 ±0,80 0,20 0,20 Flex 200
10 ±0,30 ±3,00 0,10 1,00
20-200 μl 200 ±1,2 ±0,60 0,4 0,20 Flex 200
20 ±0,36 ±1,80 0,14 0,70
30-300 μl 300 ±1,8 ±0,60 0,6 0,20 Flex 300
30 ±0,45 ±1,50 0,18 0,60
100-1000 μl 1000 ±6,0 ±0,60 2,0 0,20 Flex 1000
100 ±1,0 ±1,00 0,6 0,60
0,5-5 ml 5000 ±25,0 ±0,50 10,0 0,20 5 ml
500 ±10,0 ±2,00 4,0 0,80
1-10 ml 10000 ±50,0 ±0,50 20,0 0,20 10 ml
1000 ±20,0 ±2,00 8,0 0,80
Finnpipette F1 Fixed Volume Single Channel
Fixed Volume Inaccuracy Imprecision Factory
μl μl % s.d. μl cv% calibration tip
10 ±0,090 ±0,90 0,080 0,80 Flex 200
20 ±0,14 ±0,70 0,10 0,50 Flex 200
25 ±0,15 ±0,60 0,125 0,50 Flex 200
50 ±0,30 ±0,60 0,20 0,40 Flex 200
100 ±0,40 ±0,40 0,30 0,30 Flex 200
200 ±0,80 ±0,40 0,60 0,30 Flex 200
250 ±1,0 ±0,40 0,75 0,30 Flex 1000
500 ±1,5 ±0,30 1,5 0,30 Flex 1000
1000 ±3,0 ±0,30 3,0 0,30 Flex 1000
2000 ±6,0 ±0,30 4,0 0,20 5 ml
3000 ±9,0 ±0,30 6,0 0,20 5 ml
4000 ±12,0 ±0,30 8,0 0,20 5 ml
5000 ±15,0 ±0,30 10,0 0,20 5 ml
10000 ±30,0 ±0,30 20,0 0,20 10 ml
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