Thermo Scientific Thermo Scientific HyPerforma User manual

HyPerforma Glass Bioreactor Quick Start Guide
This guide provides a brief overview of assembling and disassembling the Thermo Scientific™HyPerforma™
glass bioreactor. Contact your sales representative for more detailed information about this product.
Assembling the Glass Bioreactor vessel
1. Using both hands, carefully place the glass
vessel into the stand (Figure 1). Ensure that the
glass vessel is centered properly.
2. Inspect the headplate assembly, ensuring that
the O-ring is clean, undamaged, and properly
installed in the groove of the headplate. Install
the headplate assembly, ensuring that the
guide pins are aligned with the headplate
guide holes (Figure 2). Ensure that none of
the installations of the headplate assembly
(cooling coil, impeller shaft, etc.) hit the glass
rim when placing the headplate assembly
onto the glass vessel. Note: Ensure that the
O-ring on the flange around the glass vessel
(as received) is intact when removing the
headplate for cleaning purposes.
3. Hand-tighten the four headplate retaining
screws (Figure 2) in a cross-wise pattern.
Note: The headplate retaining screws should
only be hand-tightened; using tools to tighten
the screws may lead to glass vessel damage.
4. Replace any septa (Figure 2) that have been
removed, used, or damaged.
5. Replace the heating jacket (Figure 1) and
ensure it is held in place with hook and loop
fastener (Velcro™) straps. Note: Both the
jacket and the straps MUST be under the
stand struts (Figure 1) in order to ensure full
contact of the jacket on the glass vessel wall.
6. Replace the agitator motor assembly. Place
the agitator motor assembly onto the agitator
bearing housing so that it is fully seated on the
headplate (Figure 1).
Figure 1. Assembled Glass Bioreactor.
Figure 2. Close-up view of headplate.
Agitator motor
assembly
Stand struts
Heating
jacket
Jacket
cable
Headplate
assembly
Retaining
screws
Guide pins/
holes
Septa port
Retaining
screws

2
7. Connect the jacket cable (Figure 1) to the
corresponding connector of the controller.
It is highly recommended that users routinely
inspect glass vessels for damage, and
replace the vessels if damage is observed.
Disassembling the Glass Bioreactor vessel
1. While supporting the vessel with one hand,
pull up on the agitator motor assembly (Figure
1) to remove. Set the motor assembly aside,
as it MUST NOT be washed.
2. Uncouple the jacket cable (Figure 1). Loosen
the hook and loop fastener (Velcro) straps on
the heating jacket (Figure 1) and remove. Set
the heating jacket aside, as it MUST NOT be
washed.
3. Remove any septa that are in place, and
replace the septa fitting. Remove all filters and
tubing from the headplate assembly (Figure 2).
4. Unscrew the four headplate retaining screws
(Figure 2).
5. Lift the headplate assembly from the glass
vessel. Some pressure may need to be
applied to release the headplate seal. We
recommend that a second operator support
the vessel stand if this is required. Ensure
that none of the installations of the headplate
assembly (cooling coil, impeller shaft, etc.) hit
the glass rim. CAUTION: DO NOT USE ANY
SHARP TOOLS to separate the headplate
assembly from the glass vessel.
6. Remove the glass vessel from the stand.
Store the glass vessel inverted (Figure 3) or on
its side, if adequately supported, to prevent
rolling. CAUTION: DO NOT store the vessel
with the base on the bottom. Because the
base is not flat, the glass vessel can
easily tip over and become damaged.
7. Under the flange of the glass vessel is an
O-ring for centering the headplate’s position
with respect to the glass vessel (Figure 3).
Ensure that the O-ring on the flange around
the glass vessel (as received) is intact when
removing the headplate for cleaning purposes.
8. Depending on the cleaning procedure, the
headplate assembly may be placed in the
stand for washing (Figure 4), but ensure that
all surfaces can be easily cleaned and that the
used cleaning agents drain off.
Figure 3. Glass vessel with O-ring,
stored inverted.
O-ring

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9. The equipment may now be washed
according to site procedure. Care should be
taken to minimize contact with other items to
prevent potential damage to the glass vessel.
See Figure 5 for the full assembly drawing,
showing the headplate assembly, stand, and
glass bioreactor.
Figure 4. Headplate assembly in stand.
Figure 5. Complete assembly
drawing—exploded view.