Thermo Scientific 1300 Series User manual

1300 Series A2
Class II, Type A2 Biological Safety Cabinet
Operating anual 7001344 Rev. 5
User Manual
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Thermo Scientific
MANUAL NUMBER 7001344
5 ECN -001017 06/24/2020 Added aintenance Notes in page 6-1. TFI
4 31573 10/2/14 Added FP in Testing Information (pg 8-10) ccs
3 28381 3/2/12 Updated to current production ccs
2 27594 10/20/11 Revised per B Bruker/George Hou ccs
1 27058/HD-1722 2/10/11 Titled General Notes--Safety, added intended use info, noise db to 65 from 63 ccs
0 -- 2/16/10 Original ccs
REV ECR/ECN DATE DESCRIPTI N By
Preface
1300 Series A2 i
This manual covers the models shown below:
Dear User,
Congratulations on your purchase of a Thermo Scientific 1300 Series Class II, Type A2
biological safety cabinet. Your six-foot 1300 Series A2 biological safety cabinet has been
certified in accordance to YY 0569-2005, and your four-foot 1300 Series A2 biological
safety cabinet has been designed in accordance to S DA standards. These cabinets were
all designed to protect the user, the environment, and your research from harmful
substances and cross-contamination. This user manual provides instructions on how to
use the 1300 Series A2 most effectively and safely.
The 1300 Series A2 offers a unique range of product features that will enhance your
safety and improve overall operating efficiency. Should you have any questions on using
this product, or need further explanation of any of it’s features, contact your local
distributor.
1344 1.2 m 230 V, China
1349 1.8 m 230 V, China
1374* 1.2 m 230 V, China
1379* 1.8 m 230 V, China
* Includes cabinet, adjustable height stand, UV light and armrest.
Registr tion Number: SFDA(I)20113543141
Registr tion St nd rd Number: YZB/USA 3532/2011

Thermo Scientificii 1300 Series A2
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. ailure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo isher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
© 2020 Thermo isher Scientific. All rights reserved.

Thermo Scientific 1300 Series A2 iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
The agents used and/or work performed in this cabinet may be hazardous to your health and the
health of others. (The cabinet must be used properly. You must read the operators manual before
using.)
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2012/19/EU (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2012/19/EU. It is marked with the WEEE symbol. Thermo isher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. urther
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo Scientific products will be available at www.thermofisher.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.
General Notes Safety

Thermo Scientificiv 1300 Series A2
General Notes
Safety
Intended use:
The 1300 series Class II, Ttype A2 biological safety cabinet can be used in clinical laboratories, is designed to
protect the user, the sample and the environment, for users to operate the sample inside the chamber through
the front operation window.
Depending on the hazard level of the agents involved, the operator must prepare in writing appropriate decont-
amination procedures for the device and the accessories used in the sample chamber.
Unintended use:
The unit must not be operated as a Class II safety cabinet, if:
- no repeat test is performed after changes to the installation conditions or after modifications to the technical
system,
- the alarm system of the device has issued a failure message and the cause for the failure has not been repaired.
The alarm system must not be tampered with or disabled. If alarm system components have been removed or
disabled for service or repairs, the unit must only be released for operation if all alarm system components are
functioning again properly.
The filters installed in the device are not capable of separating gaseous substances. Therefore, do not work or
store substances in the device:
- which in quantity or concentration are toxic,
- if a reaction with other substances may result in hazardous toxic concentrations or formation of toxic gases,
- that may form combustible or explosive mixtures in combination with air.

1300 Series A2 vThermo Scientific
Table of Contents
General Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .iii
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .iv
Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Safety Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
HEPA ilters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Paper Catch Grids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Use of the Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Unit Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Chamber Lighting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Work Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Optional Exhaust Transition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Exhaust System Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Moving the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Service Valve Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unit w/wo Stand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unlock Counterweight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Installation Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
peration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Calibration Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Unit Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Operating Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Loading the Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Working Recommendations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-6
Procedure Interrupt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7
Unit Shut-down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-8
Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-8
Section 1
Section 2
Section 3
Section 4

vi 1300 Series A2 Thermo Scientific
Cleaning / Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Wipe/Spray Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Pre-disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
inal Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
UV Disinfection after Wipe/Spray Disinfection . . . . . . . . . . . . . . .5-3
Disinfection with ormaldehyde . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean Exterior Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean the Drain Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean the Paper Catch Grid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Sample Chamber Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Optional UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Replacements and Repairs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Certification Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Data Log/Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Table of Contents
Section 5
Section 6
Section 8
Section 7
Section 9

1300 Series A2 1-1Thermo Scientific
Section 1 Description
The plenum assembly consists of a plenum for the downflow blower and a
plenum for the exhaust air blower. Each blower includes a filter. The
exhaust air is discharged through an opening in the top of the cabinet.
Chamber lighting includes two fluorescent bulbs.
The optional, factory-installed UV light is installed in the ceiling at the
front of the sample chamber.
Three service valve access ports are located on each side panel. When
installing service valves, remove the center of the port plugs at the
perforations.
Figure 1-1. Unit Components
Plenum
Power cord
Electrical enclosure
Light dome
Control panel
Handle
Front window
Service valve
access ports
Stand (optional)
Service
valve
Exhaust
test hose
Work
tray
Armrest
Supply
test hose
Accessory
outlets
Plenum
access panel
Downflow blower
Exhaust blower
RS-232
Connection
Fuseholders (2)
Exhaust opening
Smart Port

1-2 1300 Series A2 Thermo Scientific
Section 1
Description
Safety Systems
The optional stand is may be ordered in two configurations; a manually
adjustable from 750 mm to 950 mm working height, and a fixed 864 mm
working height with attached casters..
A stainless steel one-piece work surface is standard. Optional armrests
ensure a comfortable working position for the user and minimize blockage
of airflow at the front grille.
Internal outlets, provided as the power source for accessories, are provided
standard, a single outlet on each side.
Test hoses (aerosol challenge ports) are located under the work tray.
The supply plenum hose is located on the left and the exhaust plenum hose
on the right.
Note Do not remove the caps of the two test hoses to check for supply and
exhaust airflow. s
Neg tive pressure ir system
A negative pressure air system combines with HEPA filters in the supply
and exhaust airflow for personal and product protection.
Personnel protection
Inflow air along the entire working opening at a constant high velocity
prevents leakage from the work opening of the chamber.
Exterior air pressure being higher than the internal air pressure creates
negative pressure, ensuring containment in case of cabinet leakage.
Product protection
Steady airflow within the air system ensures constant downflow, allowing
the HEPA filters to remove contaminants so that the samples are always
surrounded by ultra-pure air. Harmful particles are not carried over the
sample chamber (protection from cross-contamination).
HEPA filters
The downflow air within the chamber and the exhaust air are cleaned by
HEPA filters.
S fety lockout
To protect from UV radiation, the optional UV light will not turn on if
the front window is open. While the UV light is on, the front window is
closed and locked. The closed window blocks out the UV rays. Opening
the window will cause the UV light to turn off.

1300 Series A2 1-3Thermo Scientific
Section 1
Description
Digit l Airflow Verific tion (DAVe)
Independent monitoring of inflow and downflow air velocities
guarantees that product and personnel protection remain
uncompromised. Airflow monitoring determines the velocity of the
airflow in the sample chamber as well as the inflow velocity of air
through the exhaust opening. As soon as airflow velocities rise above or
fall below a specified safety value, an audible and a visual alarm is
activated.
Window position monitoring
The position sensors detect the size of the front window opening and
indicate whether the window is open to the specified work position,
closed (energy saving) or in an unsafe intermediate position.
Thermo Perform nce F ctor
The Thermo Performance actor (PER) is a value that indicates the
safety status of the cabinet. This value is calculated from data
determined by HEPA filter loading, airflow status and values captured
during certification. This data is entered into a parameter list of the
control software and interconnected. The result is indicated on the
display by LEDs.
On certification by qualified personnel with a new HEPA filter, the
performance factor of the unit is indicated by green-green LEDs. As the
unit ages with use, the colors change. Red-red is unacceptable and the
unit should not be used. Any questions as to the safety of the unit
should be resolved before use.
Safety Systems
(continued)