Thermo Scientific Heraeus Pico 21 User manual

Thermo Scientific
Heraeus Pico 17 / 21
Instructions for Use
50133388-4 • 03/ 2020
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WEEE Conformity
This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment
(WEEE) Directive 2012/19/EU. It is marked by the following symbol:

Thermo Scientific Heraeus Pico 17 / 21 i
T
Preface ....................................................................................................................................... iii
Scope of Supply ....................................................................................................................... iii
Intended Use ..............................................................................................................................iv
Accident Prevention .................................................................................................................iv
Precautions .................................................................................................................................v
Chapter 1 Introduction and Description ............................................................................................... 1-1
Characteristics of the Heraeus Pico 17 / 21 .............................................................. 1-2
Technical Data ........................................................................................................ 1-2
Directives, Standards and Guidelines ...................................................................................... 1-4
Functions and Features ............................................................................................................ 1-4
Mains Supply ........................................................................................................... 1-5
Rotor Selection ........................................................................................................ 1-5
Chapter 2 Before Use ............................................................................................................................... 2-1
Before Setting up ..................................................................................................... 2-2
Transporting the Centrifuge .................................................................................... 2-2
Location .................................................................................................................. 2-2
Leveling the Centrifuge ............................................................................................ 2-3
Mains Connection ................................................................................................... 2-3
Storage ..................................................................................................................... 2-4
Chapter 3 Control Panel .......................................................................................................................... 3-1
Control Panel .......................................................................................................... 3-2
Keys ......................................................................................................................... 3-2
Chapter 4 Operation ................................................................................................................................. 4-1
Switch on Centrifuge ............................................................................................... 4-2
Lid Opening ............................................................................................................ 4-2
Close Door .............................................................................................................. 4-2
Rotor Installation ..................................................................................................... 4-2
Entering Parameters ................................................................................................. 4-4
Centrifugation ......................................................................................................... 4-6
Short-term Centrifugation ....................................................................................... 4-7
Removing the Rotor ................................................................................................ 4-8
Aerosol-tight Rotors ................................................................................................. 4-8
Audible Alarm ......................................................................................................... 4-8
Turning off the Centrifuge ...................................................................................... 4-9
Chapter 5 Maintenance and Care ......................................................................................................... 5-1
Table of Contents

Inhalt
ii Heraeus Pico 17 / 21 Thermo Scientific
Cleaning Intervals .................................................................................................... 5-2
Cleaning .................................................................................................................. 5-2
Disinfection ............................................................................................................. 5-3
Decontamination ..................................................................................................... 5-4
Autoclaving .............................................................................................................. 5-5
Service of Thermo Fisher Scientific .......................................................................... 5-5
Shipping and Deposing of Accessories ..................................................................... 5-6
Chapter 6 Troubleshooting ..................................................................................................................... 6-1
Mechanical Emergency Door Release ...................................................................... 6-2
Troubleshooting by User ......................................................................................... 6-3
When to contact a Service Technician ..................................................................... 6-6
Chemical Compatibility Chart ...............................................................................A-1
Index ..........................................................................................................................S-1

Thermo Scientific Heraeus Pico 17 / 21 iii
P
Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty.
Scope of Supply
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
Article Number Quantity Check
Centrifuge Heraeus Pico 17 / 21 1
Power supply cable 1
Rotor 1
20360104 Square box wrench 1
50134706 CD with Manual 1
This symbol refers to general hazards.
WARNING means that injuries or material damage or contamination could occur.
CAUTION means that material damage could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to hot surfaces.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

PIntended Use
iv Heraeus Pico 17 / 21 Thermo Scientific
Intended Use
•This centrifuge is a laboratory product designed to separate components by generation of a
relative centrifugal force. It separates human samples (e.g. blood, urine and other body fluids)
collected in appropriate containers, either alone or after addition of reagents or other additives.
•As general-purpose centrifuge, it is designed to also run other containers filled with chemicals,
environmental samples and other non-human body samples.
•Together with the hematocrit rotor 75003473 the centrifuge can be used as an in-vitro
diagnostics device to define the hematocrit value by centrifugation. Apply DIN 58933 to define
the hematocrit value.
•The centrifuge is to be used for separating materials of different density or particle size
suspended in a liquid.
Maximum sample density at maximum speed: 1.2
Accident Prevention
g
cm3
-----------
WARNING If a hazardous situation occurs, turn off the power supply to the centrifuge
and leave the area immediately.
WARNING In case of rotor failure the centrifuge can be damaged. The coolant can
escape. Ventilate the room well and leave it. Inform customer service.
WARNING Plug the centrifuge only into sockets which have been properly grounded.
Prerequisite for the safe operation of the Heraeus Pico 17 / 21 is a work environment in
compliance with standards, directives and trade association safety regulations and proper
instruction of the user.
The safety regulations contain the following basic recommendations:
•Maintain a radius of at least 30 cm around the centrifuge.
•Implementation of special measures which ensure that no one can approach the
centrifuge for longer than absolutely necessary while it is running.
•The mains plug must be freely accessible at all times. Pull out the power supply plug or
disconnect the power supply in an emergency.

P Precautions
Thermo Scientific Heraeus Pico 17/21 v
Precautions
In order to ensure safe operation of the Heraeus Pico 17 / 21, the following general safety
regulations must be followed:
•The centrifuge should be operated by trained specialists only.
•The centrifuge is to be used for its intended use only.
•While handling centrifuge, rotor, and samples you must wear laboratory clothing (e.g.
gloves).
•Do not move the centrifuge while it is running.
•Do not lean on the centrifuge.
•Use only rotors and accessories for this centrifuge which have been approved by Thermo
Fisher Scientific. Exceptions to this rule are commercially available glass or plastic
centrifuge tubes, provided they have been approved for the speed or the RCF value of
the rotor.
•Do not use rotors which show any signs of corrosion and/or cracks.
•Do not touch the mechanical components of the rotor and do not make any changes to
the mechanical components.
•Use only with rotors which have been properly installed. Follow the instructions in
section “Rotor Installation” on page 4-2.
•Use only with rotors which have been loaded properly. Follow the instructions given in
the rotor manual.
•Never overload the rotor. Follow the instructions given in the rotor manual.
•Never open the lid until the rotor has come to a complete stop and this has been
confirmed in the display.
•The lid emergency release may be used in emergencies only to recover the samples from
the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door
Release” on page 6-2).
•Never use the centrifuge if parts of its cover panels are damaged or missing.
•Do not touch the electronic components of the centrifuge or alter any electronic or
mechanical components.
•Please observe the safety instructions.

PPrecautions
vi Heraeus Pico 17 / 21 Thermo Scientific
Please pay particular attention to the following aspects:
•Location: well-ventilated environment, set-up on a level and rigid surface with adequate
load-bearing capacity.
•Rotor installation: make sure the rotor is locked properly into place before operating the
centrifuge.
•Especially when working with corrosive samples (salt solutions, acids, bases), the
accessory parts and vessel have to be cleaned carefully.
•Always balance the samples.
Centrifuging hazardous substances:
•Do not centrifuge explosive or flammable materials or substances which could react
violently with one another.
•The centrifuge is neither inert nor protected against explosion. Never use the centrifuge
in an explosion-prone environment.
•Do not centrifuge inflammable substances.
Remaining risk: Improper use can cause damages, contamination, and injuries
with fatal consequences.
•Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms
without suitable safety precautions.
When centrifuging microbiological samples from the Risk Group II (according to the
Bio-safety Manual" of the World Health Organization (WHO)), aerosol-tight biological
seals have to be used.
For materials in a higher risk group, extra safety measures have to be taken.
•If toxins or pathogenic substances have gotten into the centrifuge or its parts,
appropriate disinfection measures have to be taken (see “Disinfection”on page 5-3).
Remaining risk:Improper use can cause damages, contamination, and injuries
with fatal consequences.
•Highly corrosive substances which can cause material damage and impair the
mechanical stability of the rotor, should only be centrifuged in corresponding protective
tubes.

Thermo Scientific Heraeus Pico 17 / 21 1-1
1
Introduction and Description
Contents
•“Characteristics of the Heraeus Pico 17 / 21” on page 1-2
•“Technical Data” on page 1-2
•“Directives, Standards and Guidelines” on page 1-4
•“Functions and Features” on page 1-4
•“Mains Supply” on page 1-5
•“Rotor Selection”on page 1-5

1-2 Pico 17 / 21 Thermo Scientific
1Introduction and Description
Characteristics of the Heraeus Pico 17 / 21
Characteristics of the Heraeus Pico 17 / 21
Several rotors with a wide range of tubes can be used.
The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and
low-vibration operation even at high speeds, and guarantees a very long lifetime.
The user-friendly control panel makes it easy to pre-set the speed, RCF value, run time, and
temperature. You can choose between the display of speed and RCF or the entry mode.
These settings can be changed even while the centrifuge is running.
With the help of the key , you can also centrifuge a sample for just a few seconds, if called for.
The Heraeus Pico 17 / 21 is equipped with various safety features:
•The housing is made of impact-resistant plastic and the interior of armor steel.
•The door is equipped with a view port and a lock.
•The centrifuge door can only be opened while the centrifuge is switched on and the rotor has come
to a complete stop. The centrifuge cannot be started until the door has been closed properly.
•Door emergency release: For emergencies only, e.g. during power failures (see “Mechanical
Emergency Door Release” on page 6-2)
Technical Data
The technical data of the Heraeus Pico 17 / 21 is listed in the following table.
Table 1-1. Technical data Heraeus Pico 17
Feature Value
Environmental Conditions -Use in interior Spaces
-Altitudes of up to 2,000 m above Sea Level
-Max. relative Humidity 80 % up to 31 °C; decreasing linearly up
to 50 % relative Humidity at 40 °C.
Permissible Ambient Temperature during
Operation
+5 °C to +40 °C +5 °C to +40 °C +5 °C to +40 °C
Permissible Ambient Temperature during
Storage and Transport
-10 °C to +50 °C -10 °C to +50 °C -10 °C to +50 °C
Overvoltage Category II II II
Pollution Degree 2 2 2
Heat Dissipation Heraeus Pico 17 Heraeus Pico 17 Heraeus Pico 17
230 V 120 V 100 V
614 BTU/h 614 BTU/h 580 BTU/h
IP 20 20 20
Run Time unlimited unlimited unlimited

1 Introduction and Description
Technical Data
Thermo Scientific Heraeus Pico 17 / 21 1-3
Max Speed nmax 13300 rpm
(depending on the
Rotor)
13300 rpm
(depending on the
Rotor)
13300 rpm
(depending on the
Rotor)
Min Speed nmin 300 rpm 300 rpm 300 rpm
Maximum RCF-Value at nmax 17,000 x g 17,000 x g 17,000 x g
Maximum Kinetic Energy <1.90 kNm <1.90 kNm <1.90 kNm
Noise Level at maximum Speed < 56 dB (A) < 56 dB (A) < 56 dB (A)
Dimensions
Height
Width
Depth
230 mm
240 mm
350 mm
230 mm
240 mm
350 mm
230 mm
240 mm
350 mm
Weight with emptyRotor 10.5 kg 10.5 kg 10.5 kg
Table1-2.Technical data Pico 21
Feature Value
Environmental Conditions -Use in interior Spaces
-Altitudes of up to 2,000 m above Sea Level
-max. relative humidity 80 % up to 31 °C; decreasing linearly up
to 50 % relative humidity at 40 °C.
Permissible Ambient Temperature during
Operation
+5 °C to +40 °C +5 °C to +40 °C +5 °C to +40 °C
Permissible Ambient Temperature during
Storage and Transport
-10 °C to +50 °C -10 °C to +50 °C -10 °C to +50 °C
Overvoltage Category II II II
Pollution Degree 2 2 2
Heat Dissipation Pico 21 Pico 21 Pico 21
230 V 120 V 100 V
785 BTU/h 751 BTU/h 785 BTU/h
IP 20 20 20
Run Time unlimited unlimited unlimited
Max Speed nmax 14800 rpm
(depending on the
Rotor)
14800 rpm
(depending on the
Rotor)
14800 rpm
(depending on the
Rotor)
Min Speed nmin 300 rpm 300 rpm 300 rpm
Maximum RCF-Value at nmax 21,100 x g 21,100 x g 21,100 x g
Maximum Kinetic Energy <2.35 kNm <2.35 kNm <2.35 kNm
Noise level at maximum Speed < 56 dB (A) < 56 dB (A) < 56 dB (A)
Dimensions
Table1-1.Technical data Heraeus Pico 17
Feature Value

1Introduction and Description
Directives, Standards and Guidelines
1-4 Heraeus Pico 17 / 21 Thermo Scientific
Directives, Standards and Guidelines
Height
Width
Depth
230 mm
240 mm
350 mm
230 mm
240 mm
350 mm
230 mm
240 mm
350 mm
Weight with empty Rotor 10.5 kg 10.5 kg 10.5 kg
Table 1-2. Technical data Heraeus Pico 21
Feature Value
Table 1-3. Directives, Standards and Guidelines
Region Directive Standard
Europe
220-230 V, 50 / 60 Hz
230 V, 50 / 60 Hz
98/79/EC In Vitro Diagnostics
2011/65/EU RoHS
Directive on the Restriction of the use of certain
Hazardous Substances in electrical and
electronic equipment
Protective goals of:
2006/42/EC Machinery
2014/35/EU Low Voltage
2014/30/EU Electromagnetic Compatibility (EMC)
EN 61010-1
EN 61010-2-020
EN 61010-2-101
EN 61326-1 Class B
EN 61326-2-6
EN ISO 14971
EN ISO 13485
USA & Canada
220-230 V, 50 / 60 Hz
120 V, 60 Hz
FDA listed
Product code JQC
centrifuges for clinical use
Device class 1
ANSI/UL 61010-1
UL 61010-2-020
UL 61010-2-101
EN ISO 14971
EN ISO 13485
Japan
100 V, 50 / 60 Hz
China
230 V, 50 / 60 Hz
208-240 V, 50 / 60 Hz
CFDA listed
IEC 61010-1
IEC 61010-2-020
IEC 61010-2-101
IEC 61326-2-6
IEC 61326-1 Class B
EN ISO 14971
EN ISO 13485

1 Introduction and Description
Functions and Features
Thermo Scientific Heraeus Pico 17 / 21 1-5
Functions RCF, Temperature, and pre-temp Selection
Door Lock Automatic Door Closing and Locking starting from an initial hold Position
Mains Supply
The following table contains an overview of the electrical connection data for the Heraeus Pico 17 / 21.
This data is to be taken into consideration when selecting the mains connection socket.
Table1-5.Electrical connection data
Cat. Mains
Voltage Frequency Rated Current
Power
Consumption Equipment Fuse Building Fuse
75002410 Heraeus
Pico 17 230 V ±10% 50 / 60 Hz 1.4 A 180 W 4 A, 2-pole circuit
breaker 16 AT
75002411 Heraeus
Pico 17 120 V ±10% 60 Hz 2.6 A 180 W 8 A, 2-pole circuit
breaker 15 AT
75002412 Heraeus
Pico 17
100 V ±10% 60 Hz 2.9 A 170 W 8 A, 2-pole circuit
breaker
16 AT
75002415 Heraeus
Pico 21 230 V ±10% 50 / 60 Hz 1.7 A 230 W 4 A, 2-pole circuit
breaker 16 AT
75002416 Heraeus
Pico 21 120 V ±10% 60 Hz 3.4 A 220 W 8 A, 2-pole circuit
breaker 15 AT
75002417 Heraeus
Pico 21
100 V ±10% 60 Hz 3.9 A 230 W 8 A, 2-pole circuit
breaker
16 AT
Functions and Features
The following table gives an overview of the important functional and performance characteristics of
the Heraeus Pico 17 / 21.
Table 1-4. Functions and Features
Component / Function Description / Features
Structure / Housing Sheet Metal with attached Plastic Housing and Steel Chamber
Rotor Chamber Plastic
Up to 48 ml of spilled Liquid is retained in the Chamber and cannot enter the
Instrument.
Drive Induction Drive without Carbon Brushes
Keys and Display Easy-to-clean Keypad and Display Surface
Control Microprocessor-controlled
Internal Memory The most recent Data is saved
75002414
75002401
75002491
75002492
75002553
75002554

1-6Heraeus Pico 17 / 21 Thermo Scientific
Rotor Selection
The Heraeus Pico 17 / 21 is supplied with a rotor.
Various rotors are available to choose from.
The technical data of the rotors and the corresponding adapters and reduction sleeves for various
commercially available containers can be found in the corresponding rotor operating manuals.
For more information visit our website at: http://www.thermofisher.com
Microliter 24 x 2 75003424
Microliter 36 x 0.5 75003436
Dual Rotor 18 x 2 / 0.5 75003418
PCR Rotor 4 x 8 75003440
PCR Rotor 8 x 8 75003489
Hematocrit Rotor 75003473
1Introduction and Description
Rotor Selection
10 x 5 mL Rotor 75003465

Thermo Scientific Heraeus Pico 17 / 21 2-1
2
Before Use
Contents
•“Before Setting up” on page 2-2
•“Transporting the Centrifuge” on page 2-2
•“Location” on page 2-2
•“Leveling the Centrifuge” on page 2-3
•“Mains Connection” on page 2-3
•“Storage” on page 2-4

2Before Use
Before Setting up
2-2 Heraeus Pico 17 / 21 Thermo Scientific
Before Setting up
1. Check the centrifuge and the packaging for any shipping damage.
Inform the shipping company and Thermo Fisher Scientific immediately if any damage is
discovered.
2. Remove the packaging.
3. Remove the rotor transport protection. The cardboard is suppose to prevent damage due to
impact.
4. Check the order for completeness (see “Scope of Supply” on page iii).
If the order is incomplete, please contact Thermo Fisher Scientific.
Transporting the Centrifuge
•Due to its weight (see “Technical Data” on page 1-2), the centrifuge should be carried by several
people.
•Always lift the centrifuge at both sides on the bottom plate.
Figure 2-1. Lifting the centrifuge at both sides.
•The centrifuge can be damaged by impacts.
Location
The centrifuge should only be operated indoors.
The set-up location must fulfill the following requirements:
•A safety zone of at least 30 cm must be maintained around the centrifuge.
People and hazardous substances must be kept out of the safety zone while centrifuging.
•The supporting structure must be stable and free of resonance, for example a level laboratory
bench.
•The supporting structure must be suitable for horizontal setup of the centrifuge.
WARNING Always lift the centrifuge on both sides. Never lift the centrifuge by its front or
the back panel.
Transport the centrifuge if possible in its packaging and with the rotor transport
protection.

2 Before Use
Leveling the Centrifuge
Thermo Scientific Heraeus Pico 17 / 21 2-3
•The location must be free of grease and dust.
•The centrifuge should not be exposed to heat and strong sunlight.
•The set-up location must be well-ventilated at all times.
Leveling the Centrifuge
The horizontal leveling of the centrifuge must be checked every time after moving it to a different
location.
The supporting structure must be suitable for horizontal setup of the centrifuge.
Mains Connection
Figure 2-2. Mains Connection
1. Turn off the power supply switch on the back (press “0”).
2. Plug the centrifuge into grounded electrical sockets only.
3. Check whether the cable complies with the safety standards of your country.
WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.
CAUTION If the centrifuge is not level, imbalances can occur and the centrifuge can be
damaged.
Do not place anything under the centrifuge to level.
Note The centrifuge must be connected directly to the socket. Extension cords and multiple
sockets are not allowed.
Main Power Switch
Mains plug

2Before Use
Storage
2-4 Heraeus Pico 17 / 21 Thermo Scientific
4. Make sure that the voltage and frequency correspond to the figures on the rating plate.
Establish the connection to the power supply with the connecting cable.
Storage
•Before storing the centrifuge and the accessories it must be cleaned and if necessary disinfected and
decontaminated.
•Store the centrifuge in a clean, dust-free location.
•Be sure to place the centrifuge on its feet.
•Avoid direct sunlight.

Thermo Scientific Heraeus Pico 17 / 21 3-1
3
Control Panel
Contents
•“Control Panel” on page 3-2
•“Keys” on page 3-2

3Control Panel
Control Panel
3-2 Heraeus Pico 17 / 21 Thermo Scientific
Control Panel
In the control panel you find the keys and displays of the centrifuge. The main power switch is located
on the left side. All parameters can be selected and changed during operation.
Keys
The keys allow user intervention for controlling the operating mode as follows:
PULSE OPEN
STOP
START
Run Time
Speed / RCF Value
Toggle key
Key Display contents
Start
Normal start of the centrifuge.
Stop
End run manually.
Open door
Automatic release (possible only when device is switched on); Emergency
release (see “Mechanical Emergency Door Release” on page 6-2).
Pulse
By pressing the key the centrifuge starts immediately and
accelerates up to the end speed. Releasing the key initiates a stopping
process at the highest braking curves.
By pressing the key up and down you change the value in the
display. If you hold the key pressed, the display changes continuously at
first slowly and after a few seconds at an accelerated pace to the
higher or lower values. By pressing the key briefly, you increase or
decrease the speed in one step. When pressing both arrows the cursor
moves to the left.
Changing the display
mode
Use the key to change the display mode. (speed / RCF value, sample /
chamber temperature, run time counter from start or preset speed on)