Thermo Scientific Shake n Stack 6240 Operating instructions

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User Manual
Shake ‘n Stack
Hybridisation Oven
Operating and Maintenance Manual 7026240 Rev. 1

Thermo Scientific
Manual Number 7026240
1 ECNM-000224 02/06/2020 Removed model numbers 6244 & 6245 from the manual. TFI
0 25036/OV-368 8/16/11 New controller - Release 3 ccs
EV EC /ECN DATE DESC IPTION By
Preface
Shake ‘n Stack i
Models covered by this manual
Models Voltage Includes
6240 220 Shaking platform, drip tray, rotisserie
6241 110 Shaking platform, drip tray, rotisserie
6242 220 Drip tray, rotisserie
6243 110 Drip tray, rotisserie

Thermo Scientificii Shake ‘n Stack
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
© 2020 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Shake ‘n Stack iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
ot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2012/19/EU (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2012/19/EU. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo Scientific products will be available at www.thermofisher.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Shake ‘n Stack
Preface
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International customers, please contact your local Thermo Scientific distributor.

Thermo Scientific
Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Oven Temperature Monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Unpacking and Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Set the Required Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Set Up the Shaking Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Methodology of Hybridisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Place Membranes in a Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Insert Membranes in a Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Pre- ybridisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
ybridisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Washing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Washing in ybridisation Bottles . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Washing in a Sandwich Box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
otisseries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Variable Axis Rotisseries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
50ml & 15ml Tube Rotisseries . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Section 1
Section 2
Section 3
Section 4
Section 5
Shake ‘n Stack v

vi Shake ‘n Stack Thermo Scientific
Cleaning and Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Meshes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Bottles & Caps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Oven Interior/Shaking Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Use of adioactive Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Additional Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3
Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-4
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Troubleshooting Guide for Nucleic Acid Hybridisations . . . . . . . . .AI-1
Background Reduction - General . . . . . . . . . . . . . . . . . . . . . . . . . . .AI-1
Factors Resulting in igh Backgrounds . . . . . . . . . . . . . . . . . . . . . .AI-3
Factors Resulting in Signal Lower than Expected . . . . . . . . . . . . . . .AI-4
Hybidisation Bottle Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .AII-1
Bottle Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .AII-1
Table of Contents
Section 6
Section 8
Section 7
Section 9
Section 10
Appendix I
Appendix II

Shake ‘n Stack 1-1Thermo Scientific
Oven Temperature
Monitoring
Section 1 Introduction
The Thermo system of ybridising in bottles is considered by many
molecular biologists to be the best method for conducting ybridisations
with Southern, Northern, Dot, Slot or Colony Blots. Additional
information, including detailed protocols, is contained in the included
ybridisation Guide.
ybridising in bottles means that probe volumes may be significantly
reduced compared to experiments performed in conventional systems, and
the continual movement of the probe across the surface of the membrane
results in very efficient hybridisation reactions.
Protection from exposure to radiation is provided by the heavy walled
borosilicate glass bottles.
Warning Based on tests, these bottles block almost 100% of BETA
radiation emissions of a 1,000 pCi P-32 source. owever, users should
observe precautions due to induced X-ray phenomenon (Breemsstrahlung)
occurring, depending on the type(s) of radionuclides used. s
The ybridisation Oven itself provides additional shielding. In the event
of a spillage within the Oven, the stainless steel drip tray will contain up to
6.8 fl. oz. (200 ml) of liquid.
Note If the Shake ‘n Stack is not used as specified in this manual, the
protection provided by the equipment may be impaired.
All Ovens are calibrated by matching the thermistor temperature read-out,
seen on the LCD display, to the actual measurement of distilled water
contained within a ybridisation Bottle as it rotates. The temperature
measuring device used inside the calibration bottle is traceable to national
standards.
This calibration method ensures all experiments using the rotisserie meet
our temperature accuracy specification of ±1°C and indeed, due to the
rotisserie action, uniformity within the bottle is ±0.25°C. owever, it is
important to note that the temperature inside the oven chamber will vary
from point to point and therefore, the temperature of objects/vessels,
which are not placed in the rotisserie, will vary from that which is
displayed. Refer to “Setting the Required Temperature” for
recommendations on temperature setting when using shaking platforms.

1-2 Shake ‘n Stack Thermo Scientific
Section 1
Introduction
The Shake ‘n Stack is designed to be used with Thermo’s hybridisation
bottle. These high quality leak-proof containers can be used at
temperatures up to 70°C (for catalogue numbers, see Section 8).
Bottles should not be used above 70°C without taking the precaution of
releasing the pressure formed by the release of gases from solutions as they
are warmed. Simply loosening, then retightening the bottle cap at
approximately 60°C should relieve the pressure.
Caution Dropping or over-tightening lids can cause cracking and failure.
Lids should be inspected prior to each use. s
Caution Always wear gloves when handling the bottle and use the bottle
gripper provided (see Appendix II for full instructions on Bottle Care). s
Finally, the Shake ‘n Stack Oven is designed for reliability and ease of
maintenance. The rotisserie, shaking platform and drip tray can be easily
removed for cleaning.
Supply
Power rating: . . . . . . . . . . . . . . . . . . . . . . . . .250 W
Frequency: . . . . . . . . . . . . . . . . . . . . . . . . .50/60 z
Fuse rating: . . . . . . . . . . . . .110/120 V: 6.3A type T
. . . . . . . . . . . . . . . . . . .220/240 V: 1.6A type T
Ingress Protection Rating . . . . . . . . . . . . . . . . .IP22
Rotisserie speed control PCB fuse: . . . . . . . .500 mA
Note This item should not be serviced by the customer.
Working Environment: . . . . . . . . . . . .15°C to 25°C
Caution Care should be taken when lifting the Oven. It is advisable that
help is sought when removing an Oven from its packaging and when
stacking Ovens for use. s
Caution During operation, caution should be taken with moving parts
that are accessible when the Oven door is open. s
Thermo offers full service and technical support for all its products.
Alternatively, contact your local authorised distributor.
Safety Precautions
Oven Temperature
Monitoring (cont’d)

Shake ‘n Stack 2-1Thermo Scientific
Section 2 Unpacking and Installation
Shake ‘n Stack Oven
4 Leveling Feet
Shaking Tray (Models 6240, 6241)
Shaking Tray Drive Arm Assembly (Models 6240, 6241)
Shaking Tray Bracket (Models 6240, 6241)
Power Cable
Drip Tray
Note If any item is missing or damaged, contact Thermo or an authorised
Thermo distributor.
Warning If it is intended to use the Oven with radioactive isotopes, the
oven must be located in a designated Radiation Area. Radiation safety
procedures must be followed at all times. s
• In the event of spillage, refer to Section 6 for guidance on cleaning and
decontamination.
• All ybridisation Oven standard rotisseries are designed to accept the
long, medium or short ybridisation Bottles.
Once unpacked, attach leveling feet to base, and position on a flat surface.
Level the oven by adjusting the height of the leveling feet. Once leveled,
the oven is ready for use. If adding to a stack of Shake ‘n Stack Ovens
(maximum of 3), attach feet and adjust height, then position feet in the 4
indents on the top of the supporting Shake ‘n Stack Oven. Some
additional adjustment of the feet may be necessary until the ovens sit in a
stable position.
Note Shake ‘n Stack Ovens have been designed to stack 3 units high. No
attempt should be made to stack higher than this.
Connect each individual oven to the mains supply using only the
removable mains lead supplied. The mains lead should be fitted with a
10 A fuse for both 110/120 V and 220/240 V regions. The oven is
grounded via the mains lead and should only be connected to an grounded
supply. (See “Technical Specifications”).