Thermo Scientific STP420D User manual
1Thermo Fisher Scientific User Guide STP420D/STP420ES
Part of Thermo Fisher Scientific
STP420D/STP420ES
Thermo Scientific
Tissue Processor
Instruction manual – English
387791 Issue 1.1
02 Thermo Fisher ScientificUser Guide STP420D/STP420ES
03Thermo Fisher Scientific User Guide STP420D/STP420ES
STP420D/STP420ES
Thermo Scientific
Tissue Processor
Instruction manual – English
387791 Issue 1.1
Figure 1. Thermo Scientific Microm STP420ES Tissue Processor.
04 Thermo Fisher ScientificUser Guide STP420D/STP420ES
Preface
© 2012 ermo Fisher Scientic. All rights reserved.
Microm International GmbH is an ISO 9001/ISO 13485 Accredited Company
ermo Fisher Scientic is the trading name of Microm International GmbH
All other trademarks are the property of ermo Fisher Scientic and its subsidiaries
ermo Fisher Scientic makes every endeavor to ensure that the information contained in its support
documentation is correct and clearly stated but does not accept responsibility for any errors or omissions. e
development of ermo Fisher Scientic products and services is continuous. Make sure that any published
information that you use for reference is up to date and relates to the status of the product. If necessary, check
with ermo Fisher Scientic or your local ermo Fisher Scientic representative.
is User Guide may not, in whole or in part, be copied, photocopied, reproduced, translated, or converted to
any electronic or machine-readable form without prior written consent of ermo Fisher Scientic.
All information included in this User Guide is proprietary and condential, and the exclusive property of
ermo Fisher Scientic. is User Guide is protected by copyright and any reproduction is prohibited. e
User Guide is for use only by individuals to whom it has been made available by ermo Fisher Scientic.
Contact Addresses
e STP420 meets the following CE Mark Requirements: In Vitro Diagnostic Directive 98/79/EC.
01/12
Microm International GmbH
Otto-Hahn-Str. 1A
69190 Walldorf
Germany
Tel: +49 6227 836-200
Fax: +49 6227 836-111
www.thermo.com/pathology
ermo Fisher Scientic
4481 Campus Drive
Kalamazoo, MI 49008
USA
Tel: +1 800 522-7270
Fax: +1 269 372-2674
www.thermo.com/pathology
®
05Thermo Fisher Scientific User Guide STP420D/STP420ES
Preface
is IVD equipment complies with the emissions and immunity requirements of
IEC 61326-2-6:2006 and DIN EN 61326-2-6:2008. is equipment complies CISPR 11 Class B.
Do not use this device in close proximity to strong electromagnetic radiation (for example unshielded
intentional RF sources), as these may interfere with the proper operation.
e electromagnetic environment must be assessed prior to the operation of the device.
is User Guide covers both models of the ermo Scientic Microm STP420 Tissue Processor: the STP420D
produced between 2006 and 2009, and the STP420ES produced from February 2010 onward.
e ermo Scientic Microm STP420 Tissue Processor is referred to throughout this, and other supporting
documents, as the STP420.
06 Thermo Fisher ScientificUser Guide STP420D/STP420ES
Dear valued customer,
ank you for your decision to buy this ermo Scientic instrument.
Before turning on the STP420 for the rst time, please read these
operating instructions carefully to familiarize yourself with its proper
operation and functions. Please store this manual next to the instrument
for later consultation.
is User Guide will be supplied with each instrument. You can order
further copies at the nearest ermo Scientic sales oce by giving the
serial number of the instrument, the number of the User Guide and the
date of issue. is User Guide is available in the following languages:
Cat. No
German: 387790
English: 387791
French: 387792
Spanish: 387793
Italian: 387893
Please check the serial number on the type plate, which is placed on the
left side panel of your instrument and enter this number here. is way,
questions and service can be handled faster.
Serial No.: _____________________________
Date of Purchase: _______ / _______ / _______
Contact Consumables: ___________________
(Phone)
Contact Maintenance: ___________________
(Phone)
Welcome
Preface
07Thermo Fisher Scientific User Guide STP420D/STP420ES
ermo Scientic herewith certies that this instrument has been tested
and checked carefully. Its technical data was veried before shipment to
be in accordance with the published specications.
e instrument complies with applicable international safety
regulations.
is ermo Scientic product is warranted against defects in material
and workmanship for a period of 1 year. Parts which prove to be
defective during the warranty period will be repaired or replaced free of
charge by ermo Scientic. No other warranty is expressed or implied.
Unauthorized modication or repair by third party persons will void the
warranty.
e warranty will expire in case of improper or wrong use of the
instrument and in case the warning and precautionary messages are not
observed. ermo Scientic is not liable for any occurring damage.
Errors and omissions excepted. Subject to amendment and
improvement without further notice.
Certification
Warranty
Preface
08 Thermo Fisher ScientificUser Guide STP420D/STP420ES
How to use this user guide
Symbols Used
Symbols Used
e User Guide is written for routine users of the STP420, and their
supervisors. It is divided into a number of chapters that tell you how
to operate the STP420, and appendices which give you further infor-
mation. Familiarise yourself with the contents of the User Guide, and
especially the section Safety Precautions below, before you start your
daily routine work.
e User Guide uses the following symbols for important safety
and operational information:
Notes:
Special instructions regarding the operation of the instrument
WARNING OR CAUTION
W C: S
. O ,
.
General DanGer
O U G .
N .
BiohazarD
T .
ChemiCal hazarD
H . R
. A
-
. T .
hot surfaCe
S ’ . T
. T
.
How to Use This
User Guide
09Thermo Fisher Scientific User Guide STP420D/STP420ES
How to use this user guide
Safety Precautions
Your safety is at risk if you do not operate the instrument in accordance
with this User Guide. You must observe the following precautions while
operating the instrument. Failure to comply with these precautions
violates safety standards and the intended use of the instrument.
ermo Scientic is not liable for misuse of the instrument and failure
to comply with basic safety requirements.
Note!
Installation and Maintenance: e instrument must be installed and
maintained by a fully authorized representative, and instruction in its use
be given, by an authorized representative. All warranties and guarantees are
null and void, if a third party installs or maintains the instrument.
instrument GrounDinG
T , -
. T
- . T -
I-
E C (IEC) . D ,
. Y
.
mains VoltaGe
N . O
.
U .
T ,
. Y
( , )
. I .
hot surfaCe
S ’ . T
.
ClosinG the proCessinG ChamBers
D :
• A
• A
• T
• T
M -
, .
Safety Precautions
10 Thermo Fisher ScientificUser Guide STP420D/STP420ES
proBlems
N -
. T -
.
flammaBle enVironments
N
. A
.
malfunCtion hazarD
T / ,
S -, T I O O. A -
-
, /
.
BioloGiCal hazarD
S -
. Y
. Y
, ,
, L B M:
W H O.
unhealthy or irritatinG suBstanCes
S
. T
-
, ,
( , ,
).
reaGents
Y
A D, A R.
CleaninG reaGent – paraffin BloCkaGes
T X C-R. O-
X ( T )
. A
X
, / .
How to use this user guide
Safety Precautions
11Thermo Fisher Scientifi c User Guide STP420D/STP420ES
is product must comply with the EU’s Waste Electrical and
Electronic Equipment (WEEE) Directive 2002/96.EC. It is marked
with the following symbol:
ermo Fisher Scienti c has contracted with one or more recycling/
disposal companies in each EU member state, and this product should
be disposed of or recycled through them.
You can nd further information on ermo Fisher’s compliance with
these directives, the recyclers in your country, and ermo Fisher
products which might help in the detection of substances subject to the
RoHS directive, at:
www.thermo.com/WEEERoHS
e User Guide uses the following conventions:
• Touch screen buttons are designated in bold, for example: “Select
Reagents”. If you are not sure what the button looks like, see
Section 1-5-1 Buttons.
• e User Guide uses a number of Note and Warning symbols to
draw your attention to important information – these are described
above.
• e User Guide tells you to ‘select’ buttons. ‘Selecting’ a screen
button is the same as clicking it with the mouse, or touching it with
your nger or stylus.
Environment
Conventions
How to use this user guide
Environment
12 Thermo Fisher ScientificUser Guide STP420D/STP420ES
Table of Contents
Chapter 1
Chapter 2
Chapter 3
Chapter 4
Chapter 5
Chapter 6
Chapters
e chapters are as follows:
Introduction
is chapter describes the equipment and its functions, identies its
parts, and explains its purpose. It also tells you how to use the touch
screen.
Setting up the STP420
is chapter describes unloading and placing the equipment, turning it
on, preparing it for operation, and selecting a variety of options, such as
language, reagent rotation and so on.
Routine Operations
is chapter tells you how to perform routine tasks not covered in
Chapter 1 or Chapter 2, including how to empty containers, replace
lters, manage and replace reagents, create and edit protocols and so on.
Troubleshooting and Errors
is chapter describes some common, easily resolved problems, error
messages, and codes. It does not cover complex technical diculties.
Cleaning and Maintenance
is chapter gives you advice about cleaning and maintaining the
instrument.
Transportation and Final Shutdown
is chapter tells you how to ship the instrument safely, and how to
dispose of it correctly when it is no longer required.
13Thermo Fisher Scientific User Guide STP420D/STP420ES
Table of Contents
Contents
Welcome 06
Certication 07
Warranty 07
How to Use is User Guide 08
Symbols Used 08
Safety Precautions 09
Environment 11
Conventions 11
Chapters 12
Introduction 16
1-1 Overview 16
1-2 Main Features 18
1-3 Interfaces 20
1-4 Operation Overview 21
1-5 Touch Screen Monitor 23
1-5-1 Buttons 25
1-5-2 Using the touch screen 28
1-5-3 Status window 30
1-6 User Proles 31
1-7 Paran Containers 32
1-8 Filter System 33
1-9 Reagents and Reagent Lists 35
1-10 Protocols 36
Setting Up the STP420 37
2-1 Unpacking the STP420 38
2-2 Selecting a Site for the STP420 39
2-3 Transporting the STP420 to Its Site 41
2-3-1 Moving the STP420 using rollers 42
2-3-2 Carrying the STP420 using the transport handles 43
2-3-3 Moving the STP420 using lifting equipment 44
2-4 Preparing the STP420 and Turning It on 45
2-4-1 Turning on the power 45
2-4-2 Filling the reagent bottles manually 46
2-4-3 Filling the paran containers 47
2-4-4 Filling the wash bottle 48
2-5 Setting the Processing Options 50
2-5-1 Selecting the language 51
2-5-2 Setting local time and date 51
2-5-3 Adjusting the audible volume 52
2-5-4 Selecting the pressure units 52
2-5-5 Selecting the temperature units 52
2-5-6 Editing processing parameters 53
2-5-7 Editing reagent groups 54
2-5-8 Remote alarm 56
2-5-9 Changing the password 56
Chapter 1
Chapter 2
14 Thermo Fisher ScientificUser Guide STP420D/STP420ES
2-6 Adjusting Reagents and Reagent Lists 57
2-6-1 Editing the predened reagent list 57
2-6-2 Creating a new reagent 59
2-6-3 Deleting a reagent 60
2-6-4 Saving the reagent list 60
Routine Operations 61
3-1 Turning the Instrument On and O 61
3-1-1 Turning the instrument on 61
3-1-2 Turning the instrument o 61
3-2 Filling and Emptying the Paran Containers 62
3-2-1 Filling the paran containers 62
3-2-2 Emptying the paran containers 62
3-3 Filter System Maintenance 64
3-3-1 Filling the wash bottle with water 64
3-3-2 Emptying water from the wash bottle 64
3-3-3 Replacing the active charcoal lter cartridge 66
3-4 Assigning Reagents and Parans to Stations 67
3-4-1 Assigning reagents 67
3-4-2 Accessing paran station functions 69
3-5 Starting a Protocol 70
3-5-1 Running a protocol 70
3-5-2 Adding more specimens while a protocol is running 73
3-6 Cleaning Baskets and Replacing Reagents 74
3-6-1 Running a cleaning cycle 74
3-6-2 Manually replacing reagents 75
3-6-3 Automatically replacing reagents 76
3-7 Editing and Creating Protocols 80
3-7-1 Editing a protocol 80
3-7-2 Protocol steps and options 83
3-7-3 Creating a new protocol 84
3-8 Exporting and Importing Protocols 88
3-8-1 Exporting a protocol 88
3-8-2 Importing a protocol 89
3-9 Paran Regeneration Program 90
3-10 Creating and Changing Quick Start Buttons 91
Troubleshooting and Errors 93
4-1 Screen Display Messages 93
4-2 Fill or Drain Problems 94
4-2-1 Station cleaning 94
4-3 Leak Tight Error 96
4-4 Rotation Error 97
4-5 Power Failures 98
4-6 Opening Chambers During Power Failures 99
4-6-1 Emergency ventilating the biopsy chamber 99
4-6-2 Emergency ventilating the rotary chamber 100
4-7 Checking for Bottle Leaks Automatically 102
4-8 Export o log les 103
Chapter 3
Chapter 4
Table of Contents
15Thermo Fisher Scientific User Guide STP420D/STP420ES
Chapter 5
Chapter 6
Appendix A
Appendix B
Appendix C
Appendix D
Appendix E
Appendix F
Cleaning and Maintenance 104
5-1 Daily Maintenance 104
5-2 Routine Maintenance 104
5-3 Rotary and Biopsy Chamber Cleaning Cycle 105
5-4 Cleaning the Surfaces of the Instrument 106
5-5 Routine Maintenance 107
5-6 Service Contract 107
Transportation and Final Shutdown 108
6-1 Transportation: Preparing for Shipment 108
6-2 Final Shutdown 110
Appendix 111
Standard Equipment 111
User and Supervisor Permissions 112
Users 112
Supervisors 113
Factory Default Settings 114
Approved Reagents 115
Technical Data 116
Preferred Protocols and Best Practices 118
Protocols 118
Tissue Grossing and Fixation 120
Wrapping 120
Cassettes 120
Index 121
Table of Contents
16 Thermo Fisher ScientificUser Guide STP420D/STP420ES
Chapter 1
Introduction
e STP420 is a highly ecient, microprocessor-controlled instrument
that automatically prepares tissue samples for laboratory testing, by
xing, dehydrating, clearing, and inltrating them with paran.
Processing is governed by protocols and reagent programs which you
create and edit.
1-1 Overview
Introduction
Overview
Figure 2a. General layout of the instrument.
[1] Touch screen
[2] Paraffin reservoir
[3] Rotary chamber
[4] Biopsy chamber
[5] Reagent Managment Panel
[6] Reagent stations
[1]
[2]
[3]
[4]
[5]
[6]
17Thermo Fisher Scientific User Guide STP420D/STP420ES
Introduction
Overview
General safety
Only skilled or specially trained personnel must operate the STP420.
You must strictly observe all the listed and marked safety measures,
regulations and hygiene measures of your laboratory.
Figure 2b. General layout of the instrument.
[1] Interfaces (type I, covered)
[2] Touch screen moveable arm
[3] Toolbox Optional interfaces
[4] Interfaces (type II)
[5] Serial number plate
[6] Main switch
Interfaces
e STP420 has a console interface, which is located either on the
left side of the device [4] or on the left side of the monitor [1].
[2]
[3]
[4]
[5]
[1]
[4]
18 Thermo Fisher ScientificUser Guide STP420D/STP420ES
1-2 Main Features
e STP420 incorporates:
• A touch screen monitor, incorporating an on-screen alpha-numeric
keypad, allowing simple operation and programming from easy-to-
follow menu displays (see Section 1-5 Touch Screen Monitor).
• Two temperature-controlled process chambers:
• e rotary chamber, with a nose cone accommodating up to six
baskets.
• e biopsy chamber, accommodating one basket.
• Ten reagent stations, and two cleaning stations for the automatic
cleaning process (see Section 1-9 Reagents and Reagent Lists).
• A heated paran reservoir with four heated paran containers (see
Section 1-7 Paran Containers).
• An enclosed exhaust system with a condensation container (wash
bottle) and active charcoal lter, to allow odor-free operation (see
Section 1-8 Filter System).
• A versatile protocol system (see Section 1-10 Protocols):
• You can dene tissue processing protocols exactly suited to the
needs of your laboratories and any special quality controls. You
can adjust these protocols easily if your laboratory requirements
change.
• e processing protocols are stored on an integrated hard disk.
e number of protocols you can store depends on the number
of steps in each one. e fewer the steps, the greater the number
of protocols you can store.
• Each protocol step can be independently programmed for
process duration, end time, and temperature, and for pressure,
vacuum or pressure/vacuum cycles.
• Protocols can easily be reloaded or edited.
• e instrument monitors protocols, and issues progress
messages during each step of the tissue processing run. You
can then view and print a summary report after the run by
connecting the USB to a remote printer.
• A delayed protocol facility for weekend or overnight runs (referred
to as ‘protocol’ throughout the manual).
Introduction
Main Features
19Thermo Fisher Scientific User Guide STP420D/STP420ES
• A battery-buered protocol and data storage.
• An acoustic and remote alarm.
• A battery-supported emergency system to secure specimens in the
event of a power failure. In this case, the battery maintains complete
system operation for ve minutes. If full power does not return,
the system goes into ’emergency’ mode. e chamber continues to
rotate for sixty minutes (Duration: 5 minutes, when paran has
been lled). If power is then still not available, the instrument goes
into shut-down mode with the tissues safely stored in reagent.
• Protection features and sophisticated software controls to guarantee
that, in the event of a power failure or other problems during
the inltration process, specimens remain undamaged and the
inltration can be completed successfully.
Introduction
Main Features
20 Thermo Fisher ScientificUser Guide STP420D/STP420ES
A console with integrated interfaces is located on the left side of the
device (see Fig. 3a) or on the left side of the monitor (see Fig. 3b).
ese are for the type II interfaces:
• Modem COM 3: For remote diagnostics and remote control of
individual functions
• LPT1: Connector provided, but not intended for use
• Mouse: Not necessary, but can be connected to the PS-2 interface
for service purposes
• Keyboard: Not necessary, but can be connected to the PS-2
interface for service purposes
• RJ45/LAN: To establish a connection to a service technician’s
laptop
• Two USB ports: For storing protocols on the USB data stick
provided
Note!
USB data sticks: Only use the USB data stick provided – others will not
work. If the USB data stick provided does not work initially, shut down the
STP420 (Section 3-1-2 Turning the instrument o), plug the USB into
the USB port and turn the device on.
• 3.5-inch oppy disk drive: For storing protocols on a 3.5-inch
(1.44 Mbyte) disk
1-3 Interfaces
Figure 3a. Interfaces (type II) on the left side of the instrument.
Introduction
Interfaces
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