Thermo Scientific 1300 Series User manual

1300 Series A2
Class II, Type A2 Biological Safety Cabinet
Operating Manual 7011355 Rev. 2

Thermo ScientificThermo Scientific
MANUAL NUMBER 7011355
2 24608/HD-1548 5/1/08 Clarified artwork and instructions for unit/stand assembly ccs
1 24300/HD-1527 11/7/07 Updated electrical ratings, changed format, added transition and valve info ccs
0 -- 9/10/07 Original (50111618) ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
1300 Series A2 i
This manual covers the models shown below:
Dear User,
Congratulations on your purchase of a Thermo Scientific 1300 Series Class
II, Type A2 bio-safety cabinet. Your 1300 Series A2 bio-safety cabinet has
been tested and certified in accordance to NSF/ANSI 49, and is designed
to protect the user, the environment, and your research from harmful
substances and cross-contamination. This user’s manual provides
instructions on how to use the 1300 Series A2 most effectively and safely.
The 1300 Series offers a unique range of product features that will enhance
your safety and improve overall operating efficiency. Should you have any
questions on using this product or need further explanation of any of it’s
features, please contact the Thermo Scientific technical support group at
the phone numbers listed on Page iv, or your local distributor.
1355 4 ft 120V
1356 4 ft 230V
1357 6 ft 120V
1358 6 ft 230V
1385* 4 ft 120V
1386* 4 ft 230V
1387* 6 ft 120V
1388* 6 ft 230V
* Includes cabinet, 30 inch fixed-height stand, UV light and armrest.

Thermo Scientificii 1300 Series A2 Thermo Scientific
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. ▲
Caution All internal adjustments and maintenance must be performed by qualified service personnel. ▲
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Scientific makes no representations or warranties with respect to this manual. In
no event shall Thermo Scientific be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
©2007 Thermo Scientific. All rights reserved.

Thermo Scientific 1300 Series A2 iiiThermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Scientific
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
Thermo Scientific’s compliance with this directive, the recyclers in your country and information on
Thermo Scientific products will be available at www.thermo.com.
✔Always use the proper protective equipment (clothing, gloves, goggles, etc.)
✔Always dissipate extreme cold or heat and wear protective clothing.
✔Always follow good hygiene practices.
✔Each individual is responsible for his or her own safety.

Thermo Scientificiv 1300 Series A2 Thermo Scientific
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
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Thermo Scientific
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Our staff can provide information on pricing and give you quotations. We can
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arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
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Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
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telephone or fax. If you wish to write, our mailing address is:
International customers, please contact your local Thermo Scientific distributor.
Sales Support
Service Support
[email protected]

1300 Series A2 vThermo Scientific
Table of Contents
Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Safety Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
HEPA Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Paper Catch Grids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Use of the Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Unit Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Chamber Lighting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Work Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Optional Exhaust Transition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Exhaust System Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Moving the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Service Valve Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unit w/wo Stand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unlock Counterweight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Installation Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
Locating a Certifier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Calibration Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Unit Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Operating Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Loading the Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Working Recommendations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-6
Procedure Interrupt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7
Unit Shut-down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-8
Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-8
Section 1
Section 2
Section 3
Section 4

vi 1300 Series A2 Thermo Scientific
Cleaning / Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Wipe/Spray Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Pre-disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Final Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
UV Disinfection after Wipe/Spray Disinfection . . . . . . . . . . . . . . .5-3
Disinfection with Formaldehyde . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean Exterior Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean the Drain Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean the Paper Catch Grid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Sample Chamber Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Optional UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Replacements and Repairs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Data Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Table of Contents
Section 5
Section 6
Section 8
Section 7

1300 Series A2 1-1Thermo Scientific
Section 1 Description
The plenum assembly consists of a plenum for the downflow blower and a
plenum for the exhaust air blower. Each blower includes a filter. The
exhaust air is discharged through an opening in the top of the cabinet.
Chamber lighting includes one fluorescent bulb for 4 foot models and two
for 6 foot models.
The optional, factory-installed UV light is installed in the ceiling at the
front of the sample chamber.
Two service valve access ports are located on each side panel. When
installing service valves, remove the center of the port plugs at the
perforations.
Figure 1-1. Unit Components
Plenum
Power cord
Electrical enclosure
Light dome
Control panel
Handle
Front window
Service valve
access ports
Stand (optional)
Service
valve
Exhaust
test hose
Work
tray
Armrest
Supply
test hose
Accessory
outlets
Plenum
access panel
Downflow blower
Exhaust blower
RS-232
Connection
Fuse holders (2)
Exhaust opening

1-2 1300 Series A2 Thermo Scientific
Section 1
Description
Safety Systems
The optional stand may be ordered in three configurations; a fixed 30”
working height, a manually adjustable 30” to 38” working height, and a
fixed 34” working height with attached casters.
A stainless steel one-piece work surface is standard. Optional armrests
ensure a comfortable working position for the user and minimize
blockage of airflow at the front grille.
Internal outlets, provided as the power source for accessories, are provided
standard in the following configuration:
1355, 1385: one duplex right side
1357, 1387: two duplex left and right side
1356, 1386: one single right side
1358, 1388: two single right and left side
Test hoses (aerosol challenge ports) are located under the work tray. The
supply plenum hose is located on the left and the exhaust plenum hose on
the right.
Note Do not remove the caps of the two test hoses to check for supply
and exhaust airflow. ▲
Negative pressure air system
A negative pressure air system combines with HEPA filters in the supply
and exhaust airflow for personal and product protection.
Personnel protection
Inflow air along the entire working opening at a constant high velocity
prevents leakage from the work opening of the chamber.
Exterior air pressure being higher than the internal air pressure creates
negative pressure, ensuring containment in case of cabinet leakage.
Product protection
Steady airflow within the air system ensures constant downflow, allow-
ing the HEPA filters to remove contaminants so that the samples are
always surrounded by ultra-pure air. Harmful particles are not carried
over the sample chamber (protection from cross-contamination).
HEPA filters
The downflow air within the chamber and the exhaust air are cleaned
by HEPA filters.

1300 Series A2 1-3Thermo Scientific
Section 1
Description
Safety lockout
To protect from UV radiation, the optional UV light will not turn on if
the front window is open. While the UV light is on, the front window is
closed and locked. The closed window blocks out the UV rays.
Airflow monitoring
Airflow monitoring determines the velocity of the airflow in the sample
chamber as well as the inflow velocity of air through the exhaust open-
ing. As soon as airflow velocities rise above or fall below a specified safety
value, an audible and a visual alarm is activated.
Window position monitoring
The position sensors detect the size of the front window opening and
indicate whether the window is open to the specified work position,
closed (energy saving) or in an unsafe intermediate position.
Thermo Performance Factor
The Thermo Performance Factor (PER) is a value that indicates the safe-
ty status of the cabinet. This value is calculated from data determined by
HEPA filter loading, airflow status and values captured during certifica-
tion. This data is entered into a parameter list of the control software
and interconnected. The result is indicated on the display by LEDs.
On certification by qualified personnel with a new HEPA filter, the per-
formance factor of the unit is indicated by green-green LEDs. As the
unit ages with use, the colors change. Red-red is unacceptable and the
unit should not be used. Any questions as to the safety of the unit
should be resolved before use.
Safety Systems
(continued)

The filter system consists of two HEPA filters for downflow (supply) and
exhaust air.
Room air is drawn into the sample chamber through the work position
window opening. The room air is drawn into the negative pressure drain
pan air duct, then
pulled into the air
plenum in the top
portion of the
cabinet. The room
air is mixed with
recirculating HEPA
filtered air from the
chamber, and filtered
proportionally by the
downflow and
exhaust filters. The
filtered air is then
supplied as ultra-pure
air into the sample
chamber of the unit,
and exhausted as
ultra-pure air into
the room
environment, or into
an external exhaust
system via an exhaust
transition.
Sectional paper catch grids are installed beneath the work tray in the air
duct between the inner and outer back wall of the unit. These grids
prevent large items such as paper towels and tissues from entering the
plenum where they may impair the function of the blowers or the filters.
The grids can be easily removed for cleaning.
1-4 Model 1300 Series A2 Thermo Scientific
Section 1
Description
HEPA Filters
Ultrapure air
Exhaust air filter
Exhaust blower
Supply blower
Supply filter
Ultrapure air
Downflow
in chamber
Blended air
Paper catch
screens
Room air
Figure 1-2. Airflow Filtering System
Paper Catch Grids

Model 1300 Series A2 1-5Thermo Scientific
Section 1
Description
The control panel is located on the front of the cabinet and consists of
keys, status indicators and a numerical display.
The display with its 5-digit panel shows the following information,
regardless of the activated operating function:
• operating hours of the device
• downflow and inflow air velocities
• remaining time of the UV disinfection routine
The keys turn functions on or off.
Status indicators show:
• window work position
• airflow
• reduced blower speed
• Thermo Performance Factor
Control Panel
Display Keys
Status Indicators
Figure 1-3. Control Panel

The sample chamber is accessible through various positions of the front
window. The working position of the window is a 10 inch opening. For
loading the chamber, the window opens fully. For decontamination and
energy saving, the window can be fully closed, during which time the
blower speed is reduced.
Note The working and loading window positions are indicated on the user
interface, as controlled by micro switches and displayed by status
indicators on the control panel. ▲
Use of the Window
1-6 1300 Series A2 Thermo Scientific
Section 1
Description
Window
Window
Pillar dress
panel
Opening height
for cleaning window
Pillar dress
panel
Closed position
Work
position
Max opening for
loading samples
1300 SERIES A2
1300 SERIES A2
Figure 1-4. Window Openings

1300 Series A2 1-7Thermo Scientific
Section 1
Description
To clean the window, the glass can be lowered beyond the closed position
for access to the upper portion.
1. Lower the window to the cleaning position.
2. Upon completion of the cleaning/service work, push the front window
back in place.
Note This position can be also used for replacing the sample chamber light
bulbs. ▲
Caution If the front window jams or is sluggish to move, do not use force!
Contact Technical Services immediately. Do not attempt to repair this
problem. ▲
The standard unit includes accessory outlets, service valve access ports, an
RS-232 connection and a remote alarm connection.
The power cord to be connected to the power source is located on the
upper back of the cabinet.
The accessory outlets (max 5A) are located on the interior chamber back
wall. There are two outlets on 4 foot units and four outlets on 6 foot units.
On top of the cabinet are the two fuseholders for the 5A circuit breaker
fuses and an RS-232 connection.
Two service valve access ports are located on each side of the cabinet. To
use these ports, remove the center perforation. Service valves are available
as optional accessories.
The remote alarm accessory can be used for external exhaust air system
controls, or a failure reporting system.
Warning If a gas burner is to be operated in the sample chamber, an
appropriate shut-off valve for the gas supply must be installed. Use only
laboratory safe burners in the sample chamber. ▲
Use of the Window
(continued)
Unit Interface

Lighting for the sample chamber includes one fluorescent bulb for 4 foot
units, and two bulbs for 6 foot. The bulb(s) are installed behind the light
canopy.
1-8 1300 Series A2 Thermo Scientific
Section 1
Description
Accessory
outlet
Service
valve
Access
ports
Power cord
RS-232
Fuses
N
L
Figure 1-5. Interfaces
Chamber Lighting
Unit Interface
(continued)

1300 Series A2 1-9Thermo Scientific
Section 1
Description
The optional, factory-
installed UV light is
installed at the top of the
front section of the sample
chamber.
The operating time of the
UV light is preset for 1
hour. The UV disinfection
procedure can be started by
pressing and holding the
“UV” key on the control
panel for 5 seconds with
the window in the closed
position. If the window is
opened, the procedure is
immediately cancelled.
Note To protect from UV radiation, the UV light can only be turned on
when the window is completely closed. ▲
The single piece work tray is provided with the cabinet. It is placed onto
the frame above the sample chamber drain pan. It can be removed by
lifting up, using the large holes in the work tray.
The working area for optimal product protection extends over the entire
width and depth of the work tray. The two optional armrests are
positioned in the center of the working area 8 inches (20cm) from each
other. To attach the armrests, insert into the first perforated track of the
work tray.
Warning Work safety is assured only if the armrests are used correctly! ▲
Work Area
UV Lights
UV light
Chamber light
Light canopy
Figure 1-6. Lights
Width
Depth
Perforation
track
Armrest
Armrest
Separation
distance
Perforation
track
Work tray
Figure 1-7. Work tray

1300 Series A2 2-1Thermo Scientific
Section 2 Installation
The operational safety and proper function of the unit depend on the
location where it is to be operated. The cabinet should be operated only in
the ambient conditions listed below.
• Up to 6562 ft. (2000m) above sea level.
• Power outlet accessible to authorized personnel only. Ideally, the outlet
should be installed above the bio-safety cabinet. The power cord is the
mains disconnect.
• Location capable of supporting the weight of the device and non-
flammable.
• If the factory-approved stand is not purchased, the holding device must
be rated for twice the cabinet weight.
• Adequate room height. For devices that are not connected to an
exhaust system, the distance between the exhaust air opening at the
device and the room ceiling must be at least 8 inches (200mm).
• Equipped with an appropriate ventilation system.
• Room temperature between 10°C and 40°C (50°F and 104°F).
• Relative humidity must not exceed 80%, up to 31°C and decreasing
linearly to 50% at 40°C.
• For indoor use only.
Room ventilation should preferably be a ventilation system that complies
with the national requirements for the application.
The inlet air and exhaust air openings of the room ventilation must be
located so that drafts do not impair the function of the bio-safety cabinet
air system.

The part numbers for the optional thimble exhaust connection are
1911316 for four foot, and 1911317 for six foot units.
The test conditions according to NSF 49/2004 were performed with the
standard version of the safety cabinet, without exhaust system accessories.
An available accessory for the bio-safety cabinet exhaust system is an
exhaust thimble connection for the connection to an on-site exhaust
system with blower.
The exhaust system components are installed to the exhaust opening on
top of the cabinet. The threaded inserts for the retaining screws are pre-
installed in the unit.
Installation on a 4 ft. model:
1. Turn the unit off and disconnect it from the power source.
2. Thoroughly clean the surfaces
around the exhaust opening and
of the accessory to be installed, to
ensure that they are free of debris.
3. Apply sealant onto the contact
surfaces.
4. Secure the accessory by tightening
the supplied retaining screws (M
5) wrench-tight.
5. Remove any excess sealant.
2-2 Model 1300 Series A2 Thermo Scientific
Section 2
Installation
Optional Exhaust
Transition
Exhaust System
Accessories
Thimble
Retaining
screws
Exhaust
opening
Threaded
inserts
Figure 2-1. 4 ft Thimble

The exhaust thimble connection is installed to the top of the cabinet using
an additional adapter. The threaded inserts for the retaining screws of the
adapter are pre-installed to the top of the cabinet.
Installation on a 6 ft. model:
1. Turn the unit off and disconnect it from the power source.
2. Clean the contact surfaces around the exhaust opening, and the upper
and lower contact surfaces of the adapter thoroughly to ensure that all
grease and dust residue are removed.
3. Secure the adapter to the pre-installed threaded inserts on top of the
cabinet using the supplied four retaining screws. Tighten the screws
hand-tight.
4. Secure the accessory by
tightening the supplied
retaining screws (M 5) wrench-
tight.
1. Place the connecting sleeve
onto the connecting opening of
the accessory and secure it with
the screws.
2. Fit the connecting pipe
(200mm / 8 in) of the on-site
exhaust air system to the
connecting sleeve and seal it
with sealant.
3. Seal the unused connecting
opening with the cover. Apply
sealant to the cover, place the
cover onto the connecting
opening at the housing and
secure it with the screws.
Model 1300 Series A2 2-3Thermo Scientific
Section 2
Installation
Connecting Sleeve
Exhaust System
Accessories (continued)
Thimble
Retaining
screws
Exhaust
opening
Threaded
inserts
Pipe
Sleeve
Cover
Sleeve
Opening
Figure 2-2. 6 ft Thimble and Sleeve