Thermo Scientific 7470 Series User manual

User Manual
Model 7450 and 7470 Series
CryoMed Controlled Rate Freezer
Operating and Maintenance Manual 7027452 Rev. 4
Visit us online to register your warranty
www.thermoscientific.com/labwarranty

Thermo Scientific
MANUAL NUMBER 7027452
4 30873/FR-2674 7/24/14 Corrected overlay part number in Parts List (Section 7) from 140296 to 140460 ccs
3 30873/FR-2674 7/22/14 RoHS overlay part number - wiring diagrams in Section 7 ccs
2 29779 5/27/14 New software V2.0 ccs
1 28702/FR-2371 8/29/12 Removed flatbed scanner references ccs
1 28816/FR-2371 8/29/12 Added ferrite - parts list, schematics, wiring diagrams ccs
0 28576/FR-2357 7/31/12 Original - Release 4 with new printer power supply ccs
0 28511/FR-2371 7/31/12 Original - Release 4 with new printer power supply ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Controlled Rate Freezer i
Non-IVF Units
Model Voltage
7450 120
7451 230
7452 120
7453 230
7454 120
7455 230
7472 100
7473 100
7474 100
IVF Units
Model Voltage
7456 120
7457 230
7458 120
7459 230
7475 100
7476 100
Models covered in this manual”

Thermo Scientificii Controlled Rate Freezer
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
©2012 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Controlled Rate Freezer iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and information
on Thermo products will be available at www.thermo.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Controlled Rate Freezer
Preface
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Our staff can provide information on pricing and give you quotations. We can
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When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
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Service Support
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Controlled Rate Freezer vThermo Scientific
Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Standard Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Door Latch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Sample ‘Seeding’ Access Ports (IVF) . . . . . . . . . . . . . . . . . . . . . . . .1-2
Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Output . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Pre-set Profiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Set-Up and Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Electrical Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Sample Thermocouple Connection . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Power-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Access Code Security . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Initiate a Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Stop a Profile Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-6
Program User Specific Profiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
Manual Override . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
Copy and Paste Pre-set and User Profiles . . . . . . . . . . . . . . . . . . . . .2-10
Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-11
Chamber Thermocouple Open . . . . . . . . . . . . . . . . . . . . . . . . . .2-12
Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-12
Sample Thermocouple Open . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-13
Heater Malfunction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-15
Chamber/Sample Over/Under Limit Temp . . . . . . . . . . . . . . . . . .2-15
Temperature Tracking Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-16
Run Complete . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-17
Power Failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-17
IVF Sample Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-18
Warm Complete . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-18
Seeding Hold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-18
Printer Buffer Full . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-18
Using the Straw Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-19
Using a Vial Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-19
Using the Cane Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-20
Holder Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-21
Section 1
Section 2

vi Controlled Rate Freezer Thermo Scientific
Configuration/Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Menu Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Menu Entry Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Disinfect Controlled Rate Freezer Interior . . . . . . . . . . . . . . . . . . . . .4-1
Clean a Major Spill in the Chamber . . . . . . . . . . . . . . . . . . . . . . . .4-2
Clean Gasket/Heat Break . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Clean the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Printer Set-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Change the Paper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Wiring Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-15
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
PC Interface Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-2
Login . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-6
Login Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-8
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-8
Run Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-9
Run File Name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-10
Information Fields . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-11
Profile Steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-12
Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-12
Chamber/Sample Temperature . . . . . . . . . . . . . . . . . . . . . . . . . .10-12
Remaining Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-12
Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-13
Remote Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-13
Graph . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-13
Graph Legend . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-14
Typical Run Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-14
Manual Override Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-17
End Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-18
Table of Contents
Section 3
Section 4
Section 5
Section 6
Section 8
Section 7
Section 9
Section 10

Print . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-19
Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-20
Power Failure Recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-22
Display/Control Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-23
Edit Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-24
Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-25
Create Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-28
Edit Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-29
Print a Profile Listing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-32
Display Profile Temperature Graph . . . . . . . . . . . . . . . . . . . . . . .10-32
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-33
Review Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-34
User Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-35
Options Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-39
Plot Colors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-39
Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-40
Default Directories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-40
Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-41
Username Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-42
Password Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-43
Anonymous User Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-43
Information Fields . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-43
Status Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-44
Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-45
About . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-45
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1
Handling Liquid Nitrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .AA-1
Controlled Rate Freezer viiThermo Scientific
Section 10 (continued)
Section 11
Appendix

Controlled Rate Freezer 1-1Thermo Scientific
Section 1 Introduction
Figure 1-1. Non-IVF Controlled Rate Freezer
Figure 1-2. IVF Controlled Rate Freezer (FDA clearance for human research)

1-2 Controlled Rate Freezer Thermo Scientific
Section 1
Introduction
The door of the Controlled Rate Freezer is secured in the closed position
with a vise-action T-handle latch. The latch maintains positive
compression of the door gasket against the Teflon heatbreak to prevent
leakage and ice build-up during normal operation. The latch is closed by
turning the handle 180 degrees clockwise.
Note Make sure the door is properly latched during operation to prevent
ice build-up. s
The IVF model of the Controlled Rate Freezer has top-mounted sample
access ports used to ‘seed’ the samples during the freezing process. Seeding
should always be performed through the access ports rather than the door
to reduce sample temperature fluctuations during the seeding process.
There are three displays on the Controlled Rate Freezer control panel:
Chamber and Sample temperature, ranging from +54° to -199°C, the
LCD Display for profile status, messages, and freezing profile editing and
viewing.
Standard Features
Figure 2-3. Control Panel Displays, Indicators and Keys
Door Latch
Sample ‘Seeding’ Access
Ports (IVF)
Displays

Controlled Rate Freezer 1-3Thermo Scientific
Section 1
Introduction
At the top of the control panel are two columns of LED Operating Mode
indicators. The left indicators are:
Idle . . . . . . . . . . . . . . . . . . . . . .No profile is running
Run . . . . . . . . . . . . . . . . . . . . .A profile is in progress
Wait . . .Profile in progress pauses until the Run key is pressed
End . . . . . . . . . . . . . . . . . . . . . . . .Profile is complete
Warm . . . . . . . . . . . . .Chamber is warming to +25°C
The right column of indicators are:
Cool . . . . . . . . . . . . . . . .Single LN2solenoid is open
Cool+ . . . . . . . . . . . . . .Both LN2solenoids are open
Heat . . . . . . . . . . . . . . . . . . . .The heater is energized
There are seven input keys on the control panel.
Warm . . . . . . . . . . . . . . . . . .Starts the warming cycle
Run . . . . . . . . . . . . . . . . . . .Starts chosen profile, or resumes after Wait.
Silence . . . . . . . . . . . . . . . . .Turns off the audible alarm and clears the
alarm messages from the display
3Back . . . . . . . . . . . . . . . . . .Terminates a profile in progress, returns to
Program menu, or moves the cursor to the left
4Enter . . . . . . . . . . . . . . . . . .Starts a new line, enters a sub-menu,
moves the cursor to the right, or enters View or Edit Mode
s (Up) . . . . . . . . . . . . . . . . .Scrolls upward through display lines, or
changes profile step and set-up parameters
t (Down) . . . . . . . . . . . . . . .Scrolls downward through display lines, or
changes profile step and set-up parameters
Above the Silence key is the alarm indicator. In an alarm condition, the
LED flashes in addition to an audible tone. See Alarms, Section 2.
Graphs and cycle data, as well as additional information, are printed out at
the top of the unit on a thermal printer in real-time mode.
Indicators
Keys
Alarm
Output

The Controlled Rate Freezer has six pre-set freezing profiles. They are
labeled Pre-Set Profile 1 through 6. These profiles cannot be edited.
To view the profile timing and temperature:
1. Scroll, using the up or down arrow keys, to the desired profile.
2. Press the Enter key to go into View Mode. Scroll, using the up or
down keys, through steps as needed.
3. Press the Back key to return to the main menu.
The pre-set profiles are listed below. In addition, a Configure/Calibrate
menu is available. For further information on this menu, see Section 3.
User specific profiles can also be entered and saved. See Section 2.
Pre-Set Profile #1 - Commonly used for 2.0ml sample size, resulting in a
1°C rate from nucleation to -40°C and a 10°C per minute cooling rate to
a -90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait at 4.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S to -4.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .25.0 C/m C to -40C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C to -12.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C to -40C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C to -90C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Pre-Set Profile #2 - Commonly used for very small (96 well) sample size,
resulting in a 1°C rate from nucleation to -40°C and a 10°C per minute
cooling rate to a -90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait at 4.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S to -40C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C to -90C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
1-4 Controlled Rate Freezer Thermo Scientific
Section 1
Introduction
Pre-set Profiles

Pre-Set Profile #3 - Commonly used for straw size samples, resulting in a
slow rate to -7°C, a 5 minute hold for manual cell nucleation, a 0.3°C
rate to -35°C, and then a 0.1°C per minute cooling rate to a -37°C end
temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait at 20C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C to -4.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.5 C/m C to -7.0C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .Hold for 5m at -7.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.3 C/m C to -35C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.1 C/m C to -37C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Pre-Set Profile #4 - Commonly used for hemopoietic stem cells that are
65-100ml in size, resulting in a 1°C rate from nucleation to -45°C and a
10°C per minute cooling rate to a -90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait at 20.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S to -6.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .25.0 C/m C to -50C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C to -14.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C to -45C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C to -90C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Pre-Set Profile #5 - Commonly used for skin packets, resulting in a 1°C
rate from nucleation to -35°C and a 10°C per minute cooling rate to a
-90°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait at 4.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S to -5.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .20.0 C/m C to -40C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C to -10.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C to -35C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C to -90C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
Controlled Rate Freezer 1-5Thermo Scientific
Section 1
Introduction
Pre-set Profiles
(continued)

Pre-Set Profile #6 - Commonly used for hemopoietic stem cells that are
65-100ml in size, resulting in a 0.5°C rate from nucleation to -20°C and
a 1°C per minute cooling rate to a -80°C end temperature.
Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait at 4.0C
Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S to -4.0C
Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .20.0 C/m C to -45C
Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C to -10.0C
Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.5 C/m C to -20C
Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S to -80C
Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End
S = Sample Temperature
C = Chamber Temperature
C/m = degrees Centigrade per Minute
1-6 Controlled Rate Freezer Thermo Scientific
Section 1
Introduction
Pre-set Profiles
(continued)

Controlled Rate Freezer 2-1Thermo Scientific
Section 2 Set-Up and Operation
Warning Nitrogen gas can cause suffocation without warning. Store and
use liquid nitrogen only in a well-ventilated place. As the liquid evaporates,
the resulting gas displaces the normal air in the area. (The cloudy vapor
that appears when liquid nitrogen is exposed to the air is condensed
moisture, not the gas itself. The issuing gas is invisible.) In closed areas,
excessive amounts of nitrogen gas reduces the concentration of oxygen and
can result in asphyxiation. Because nitrogen gas is colorless, odorless, and
tasteless, it cannot be detected by the human senses. Breathing an
atmosphere that contains less than 19.5% oxygen can cause dizziness and
quickly result in unconsciousness and death. Therefore, the use of oxygen
monitoring equipment is strongly recommended. s
Warning Contact of liquid nitrogen with the skin or eyes may cause
serious (freezing) injury. s
This freezer should be located in a well-ventilated area of the laboratory, on
a table/bench capable of supporting the unit’s weight (see Specifications
section), with adequate workspace available. Allow clearance for the open
door on the front of the unit, for the hose and power connections on the
back, and the exhaust vent on the left side. (Do NOT attach anything to,
or tamper with, the exhaust vent!) Locate the unit in close proximity to the
LN2source.
When closing the freezer door, be sure to turn the handle 180° in rotation
to fully seal the door.
On/Off Switch
(mains
disconnect)
Power
Inlet
Tank Hose
Connection
RS232 Interface
Figure 2-1. Connections on Unit Back

2-2 Controlled Rate Freezer Thermo Scientific
Section 2
Set-Up and Operation
Figure 2-2. Clearance for All Units

Controlled Rate Freezer 2-3Thermo Scientific
Section 2
Set-Up and Operation
Supplies The Controlled Rate Freezer requires a clean, low pressure (22 PSIG,
±30%) LIQUID nitrogen supply. Do not use the gas fitting on the supply
tank! A dedicated supply tank/source is recommended for optimum
operation. The tank hose connection, 1/2” flare fitting, is located on the
back of the unit in the bottom left corner. Do not over-tighten when
connecting. Internal plumbing could be damaged. Teflon tape is NOT
recommended when installing LN2supply. The tape cannot withstand the
low temperatures of this application. See Figure 2-1 for the connection
location.
Refer to the electrical schematics or serial data tag on the unit for electrical
specifications for the Controlled Rate Freezer.
1. Verify the power switch on the back of the unit is off.
2. Attach the power cord to the inlet on the back of the freezer. Connect
the power cord to the appropriate power outlet.
The Controlled Rate Freezer is designed to be electrically safe in the fol-
lowing environmental conditions:
• Indoors
• Altitude: Up to 2,000 meters
• Temperature: 5°C to 40°C
• Humidity: 80% RH at or below 31°C, decreasing linearly
to 50% RH at 40°C
• Mains Supply Fluctuations: ±10% of nominal.
• Installation Category II 1
• Pollution Degree 2 2
• Class of Equipment I
1 Installation category (overvoltage category) defines the level of transient overvoltage
which the instrument is designed to withstand safely. It depends on the nature of the
electricity supply and its overvoltage protection means. For example, in CAT II which
is the category used for instruments in installations supplied from a supply comparable
to public mains such as hospital and research laboratories and most industrial
laboratories, the expected transient overvoltage is 2500V for a 230V supply and
1500V for a 120V supply.
2 Pollution Degree describes the amount of conductive pollution present in the
operating environment. Pollution Degree 2 assumes that normally only non-conductive
pollution such as dust occurs with the exception of occasional conductivity caused by
condensation.
Electrical
Connections
Environmental
Conditions

Non-IVF models
1. Open the unit door.
2. Locate the thermocouple connector in the front upper right corner of
the chamber.
3. Install the supplied sample thermocouple in the correct orientation.
4. Close the unit door.
IVF models
1. Locate the thermocouple connector on the top of the unit, to the left
of the printer cover (refer to Figure 1-1).
2. Install the appropriate supplied sample thermocouple in the correct
orientation.
Note The freezer has been calibrated at the factory with the 1.2/2.0ml
sample thermocouple probe installed in the internal thermocouple
connector. See Section 3 to calibrate an external thermocouple probe. s
1. Turn the freezer power switch on the back of the unit to On.
2. On the control panel, the Alarm indicator lights briefly, and the LCD
display reads Controlled Rate Freezer XX (XX = software version).
3. The Idle indicator lights, then the LCD display shows the profile list.
4. To choose a profile, scroll through the selections using the up/down
arrow keys. The profiles are displayed in a loop. Pressing either of the
arrow keys consecutively brings the list back to the starting point.
2-4 Controlled Rate Freezer Thermo Scientific
Section 2
Set-Up and Operation
Sample Thermocouple
Connection
Power-Up

The controller will provide security for the following menus and functions,
if the Access Code is enabled, by prompting for a 4-digit numeric access
code:
• Configuration/Calibration Menu
• Profile Edit Menu
• Manual Override during a run
• Start of a run on second or subsequent step in a profile
• Manual change in Run profile execution to a prior or following step
• Premature termination of a profile run
If access code protection is enabled (see the Configuration/Calibration
section), access to the above functions is gained by entering the access code
as follows:
1. Use the Enter and the Back key to select the desired digit. Use the up
or down arrow keys to change the digit. When finished, use Enter to
confirm entry of the code and to move into the selected protected
function or menu.
2. The access code when protection is disabled is 0 0 0 0.
3. The access code can be enabled, changed, or disabled by using
“Change Access Code” (see Configuration/Calibration section).
1. Load the product into the freezing chamber. On IVF models, make
sure all unused sample access ports are capped.
2. If the profile list is not on the display, press Back until the list appears.
3. Locate the item selector (è) on the left of the top line of the menu.
4. Move the required profile to this line by using the 5/6 keys. The
chosen profile must be on the same line as the selector.
5. Press the Run key. If the chosen profile begins with a Wait, the Run
key must be pressed again to continue, after the appropriate
temperature is reached.
6. The mode indicator is lit, showing the status of the profile.
Controlled Rate Freezer 2-5Thermo Scientific
Section 2
Set-Up and Operation
Access Code Security
Initiate a Profile