THORLABS CHROLIS User manual

6-Wavelength High-Power
LED Source
Chrolis
Operation Manual
2020

Version:
Date: 1.1 28-May-2020
Copyright © 2020 Thorlabs

Foreword
Contents
1 General Information 1
11.1 Ordering Codes and Accessories 11.1.1 Light Source and Accessories 21.1.2 Optional Accessories 21.2 Requirements
2 Getting Started 3
32.1 Parts List
3 Operating Instructions 5
53.1 Operating Elements 73.2 Chrolis Setup 73.3 Connecting the Optical System 83.4 Inserting Bandpass Filters 83.5 BNC Breakout Box 93.6 Interlock 93.7 Operation 93.8 Software 103.9 Chrolis Shut Down
4 Maintenance and Service 11
5 Appendix 12
125.1 Technical Data Chrolis 135.2 Technical Data CHROLIS-C1 145.3 Technical Data CHROLIS-C2 155.4 Technical Data CHROLIS 155.5 Chrolis LEDs and Dichroic Mirrors 165.6 Safety 175.7 Warning Labels 175.8 Return of Devices 185.9 Manufacturer Address 195.10Certifications and Compliances 205.11Limited Warranty 205.12Copyright 215.13Thorlabs Worldwide Contacts - WEEE Policy

© 2020 Thorlabs
We aim to develop and produce the best solutions for your
applications in the field of optical measurement techniques. To help
us to live up to your expectations and constantly improve our
products, we need your ideas and suggestions. We and our
international partners are looking forward to hearing from you.
Thorlabs GmbH
Warning
Sections marked by this symbol explain dangers that might result in
personal injury or death. Always read the associated information
carefully before performing the indicated procedure.
Please read this advice carefully!
This manual also contains "NOTES" and "HINTS" written in this form.
Attention
Paragraphs preceded by this symbol explain hazards that could
damage the instrument and the connected equipment or may cause
loss of data.
Note

© 2020 Thorlabs
1 General Information
1
1 General Information
The Chrolis 6-Wavelength High-Power LED Source is a light source featuring up to six different
LED modules ranging from UV to IR. The light output of the LEDs is combined via dichroic mir-
rors and coupled into a Liquid Light Guide (LLG). The LLG can for example be coupled to a mi-
croscope for fluorescence imaging or any other application where multiple wavelengths of light
are of use. In addition to generating CW light, the Chrolis has extensive pulse capabilities.
The Chrolis is a modular device and allows the user to configure a light source with LED mod-
ules of different wavelengths and corresponding dichroic mirrors most suitable for the individual
application. The Chrolis is available as two pre-configured devices with preselected LEDs of dif-
ferent wavelength, the CHROLIS-C1 and CHROLIS-C2, or as a configurable item CHROLIS.
Available LED modules for the configurable CHROLIS are listed on the Thorlabs website of the
Chrolis. Further, the user can insert a bandpass filter after each LED to restrict the excitation
wavelength. The LEDs and dichroic mirrors are mounted on an actively cooled heat sink and
the temperature of the LED modules is continuously monitored to ensure highly stable perfor-
mance.
This multichannel light source is compatible with Thorlabs' Ø3 mm liquid light guides. The tip of
the liquid light guide is cooled using thermoelectric coolers (TECs) to extend the lifetime of the
LLG.
The Chrolis is controlled by the Chrolis software via a USB interface. The software recognizes
the assembled LED configuration which is then displayed with the respective spectra. The
Chrolis software allows the user to set the intensity for each LED as well as to modulate the
LEDs for pulsed light applications without the use of an external function generator.
A BNC breakout box (available separately) allows input and output of TTL signals to link the
LED modulation with the timing of external devices. The BNC Breakout Box can be configured
with the Chrolis software for the following applications: (1) direct modulation of the LEDs by an
external TTL signal, (2) triggering internal modulation by an external TTL signal, (3) generating
a TTL output signal for other devices.
Attention
Please find all safety information and warnings concerning this product in the chapter Safety
in the Appendix.
1.1 Ordering Codes and Accessories
1.1.1 Light Source and Accessories
CHROLIS-C1
Chrolis 6-Wavelength High-Power LED Source with six pre-selected
LEDs modules (peak wavelengths: 365/405/475/565/625/780 nm) and
five matching dichroic mirror modules.
CHROLIS-C2
Chrolis 6-Wavelength High-Power LED Source with six pre-selected
LEDs modules (peak wavelengths: 385/420/475/525/590/625 nm) and
five matching dichroic mirror modules.
CHROLIS
Chrolis 6-Wavelength High-Power LED Source for Custom Configura-
tions with up to six LED modules. Please visit the website and use the
online configurator to request a custom configuration. Availabe LED
Modules are listed on the Thorlabs website.
LLG03-4H
Liquid Light Guide, Ø3 mm Core, 340 - 800 nm, 1.2 m length. Included
with each Chrolis model.
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Chrolis
1.1.2 Optional Accessories
BBC1
BNC Breakout Box for input and output of TTL signals for synchroniza-
tion with external devices.
Bandpass Filter
Chrolis LEDs can be equipped with a bandpass filter to restrict the emitted
spectrum. Please find a variety of Thorlabs' bandpass filters on the
Thorlabs website: https://www.thorlabs.com/navigation.cfm?guide_id=2210
1.2 Requirements
The requirements for the PC intended to be used for remote operation of the Chrolis are as fol-
lows:
Hardware Requirements
CPU: 2.4 GHz or Faster
RAM: Min. 4 GB
Graphic Card: Min. 1024 x 768 Pixel Graphic Resolution
Hard Disc: Min. 1 GB of Available Free Disk Space (32 bit Operating System)
Min. 2.3 GB of Available Free Disk Space (64 bit Operating System)
Interface: Free USB 2.0 Port, USB Cable According the USB 2.0 Specification
Software Requirements
The CHROLIS software is compatible with the following operating systems:
·Windows®7 (SP1+) (32 bit, 64 bit)
·Windows®8.1 (32 bit, 64 bit)
·Windows®10 (32 bit, 64 bit)
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© 2020 Thorlabs
2 Getting Started
3
2 Getting Started
Inspect the shipping container for damage. Please do not cut through the cardboard. You might
need the box for storage or for returns.
If the shipping container seems to be damaged, keep it until you have inspected the contents
for completeness and tested the Chrolis mechanically and electrically.
2.1 Parts List
Please verify that you have received the following items within the package:
CHROLIS-C1
1. Chrolis 6-Wavelength High-Power LED Source
2. 6 LED Modules mounted in Chrolis: Wavelengths: 365 nm, 405 nm, 475 nm, 565 nm,
625 nm, 780 nm
3. 5 Dichroic Mirrors mounted in Chrolis
4. LLG03-4H Liquid Light Guide
5. USB 2.0 Cable for Connection of the Chrolis to the PC
6. Power Chord, country specific
7. Quick Reference

© 2020 Thorlabs4
Chrolis
CHROLIS-C2
1. Chrolis 6-Wavelength High-Power LED Source
2. 6 LED Modules mounted in Chrolis: Wavelengths: 385 nm, 420 nm, 475 nm, 525 nm,
590 nm, 625 nm
3. 5 Dichroic Mirrors mounted in Chrolis
4. LLG03-4H Liquid Light Guide
5. USB 2.0 Cable for Connection of the Chrolis to the PC
6. Power Chord, country specific
7. Quick Reference
CHROLIS
1. Chrolis 6-Wavelength High-Power LED Source
2. Up to 6 LED modules, mounted inside the Chrolis
3. Up to 5 Dichroic Mirrors, mounted inside the Chrolis
4. LLG03-4H Liquid Light Guide
5. USB 2.0 Cable for Connection of the Chrolis to the PC
6. Power Chord, country specific
7. Quick Reference

© 2020 Thorlabs
3 Operating Instructions
5
3 Operating Instructions
3.1 Operating Elements
Chrolis Side View Closed
Chrolis Side View Open

© 2020 Thorlabs6
Chrolis
Chrolis Front View
Chrolis Back View

© 2020 Thorlabs
3 Operating Instructions
7
3.2 Chrolis Setup
For basic setup of the CHROLIS, please follow these instructions:
Attention
Do not position the device such that the operation of the On/Off switches is difficult.
1. Install the Thorlabs Chrolis software on your computer.
2. Connect the Chrolis to the power outlet at the rear side of the Chrolis using the
provided power cord.
3. Connect the Chrolis to the PC using the supplied USB cable.
4. Make sure that the interlock pin is inserted. Alternatively, if you use an external inter-
lock , connect it to the 2.5 mm jack.
5. Lift the handle "RELEASE LLG" on the front side of the CHROLIS
6. Insert the Liquid Light Guide (LLG) that is connected to the light receiving optical sys-
tem. Make sure to insert the end that is labeled by a yellow band into the CHROLIS.
7. Once the LLG is inserted, release the handle.
8. Switch on the main switch at the back of the housing for general power supply.
9. Switch on the Chrolis using the power switch on the front panel.
10. Start the Chrolis software.
11. For detailed information on the software, please see the software manual available on
the software website.
Attention
Please install the software prior to connecting the instrument to a PC via the USB interface.
Use only the supplied high speed (USB 2.0) cable, not full speed (USB 1.1) cables or thin pro-
file cables with increased resistance, as this can cause transmission errors and improper instru-
ment operation!
3.3 Connecting the Optical System
The light beam generated by the Chrolis is delivered to the optical system via a Liquid Light
Guide (LLG).
Thorlabs provides the LLG03-4H which can be inserted in all optical systems with a connection
for Liquid Light Guides of a Ø3 mm core.
Please find below the instructions to connect the LLG with the Chrolis.
1. Turn off the Chrolis using the ON/OFF switch located on the front of the Chrolis.
Warning Ensure that all LEDs are off and no light is emitted when removing or inserting the
LLG as light exposure might be harmful to eyes.
2. Lift the handle "RELEASE LLG".
3. Insert the LLG into the Chrolis. Please use the end that is labeled by a yellow band.
4. Release the handle.
5. Turn on the Chrolis using the ON/OFF switch located on the front of the Chrolis.
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Chrolis
6. The presence of the LLG on the Chrolis is now electronically recognized and the tempera-
ture control is activated.
7. Use the Chrolis software to activate the LEDs.
Attention
Prior to turning on the Chrolis and using the Chrolis software, ensure that the free end of the
LLG is pointing away from all people or reflective surfaces in the room.
3.4 Inserting Bandpass Filters
The Chrolis modular setup allows the user to restrict the spectrum emitted by each LED by in-
serting a bandpass filter. The filter is positioned in a filter holder in the front part of the LED
module. The filter holder is held in place by 2 magnets on the holder side facing the LED. The
1" bandpass filter itself is inserted in the holder and secured by a SM1 retaining ring from the
LED-facing side of the holder with the magnets. The empty bandpass filter holder with the
SM1RR retaining ring is included with each LED module.
How to exchange/insert a bandpass filter:
1. Pull the filter holder in the up direction.
2. Unscrew the retaining ring using either Spanner Wrench SPW602 or SPW606 (not in-
cluded).
3. Insert the bandpass filter into the holder. Please do not touch the filter surface.
4. Secure the bandpass filter by screwing the retaining ring into the holder. Please ensure not
to overtighten the retaining ring.
5. Reinsert the filter holder into the LED module with the magnet facing the LED
Thorlabs offers a large variety of bandpass filters on the Thorlabs website. Please use filters
with Æ25 mm or Æ1" and a thickness up to 6.3 mm or 0.248".
For more information, please contact Thorlabs directly.
3.5 BNC Breakout Box
The BNC Breakout Box BBC1 (available separately) enables the user to configure the timing of
the LEDs with external devices. The BNC Breakout Box offers BNC connectors for 6 "LED"
channels and 6 additional "AUX" channels.
The channels "LED 1" to "LED 6" can be used for direct modulation of the corresponding LEDs
by external TTL signals. The AUX channels can be either configured as input or output. They
allow the output of TTL signals for synchronization with other devices or the input of external
TTL signals that can be linked to internal signals.
The BNC Breakout Box can be configured via the CHROLIS software. Please see the
CHROLIS software manual for detailed instructions.
To use the BNC Breakout Box, connect the DSUB25 connector of the BNC Breakout Box to the
CHROLIS.
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© 2020 Thorlabs
3 Operating Instructions
9
3.6 Interlock
The hardware interlock is a safety feature, accessible via the INTERLOCK connector on the
rear panel. By default, a short circuit jumper is installed to the 2.5 mm phone jack.
Instead of the jumper, an external emergency switch (opener) can be connected to the inter-
lock.
3.7 Operation
For general operation of Chrolis, install the Chrolis software, setup the system and con-
nect it to your optical system according to the instructions above.
The Chrolis is now ready for operation via the Chrolis software.
For detailed instructions on the software, please see the software manual on software tab of
the CHROLIS website.
Note The CHROLIS switches off automatically if the temperature of any LED exceeds 90°C.
3.8 Software
Download
As Thorlabs aims to contribute to saving our environment, we decided to refrain from shipping
an installation CD ROM or USB flash drive and to offer a download of the software from our
website instead. As an additional advantage to this distribution method, the most recent version
of the software will always be available online.
The Chrolis software package can be downloaded from the Thorlabs website.
Note
Do not connect the Chrolis to the PC during software installation! Please make sure that the in-
stallation is carried out completely, including the reboot requests. Exit all running applications
on your PC as the installer may require a reboot of your PC during installation!
· Save the ZIP file to your computer and unpack the archive.
· Double click the setup.exe to install Shield Wizard.
· Read and accept the End-user License Agreement.
· After installing the Chrolis software, connect the Chrolis to the PC.
Start Application
Start the Chrolis software from the desktop icon:
or access the Chrolis software from the START button:
Note
For detailed information on the software, please see the software manual.
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© 2020 Thorlabs10
Chrolis
3.9 Chrolis Shut Down
To turn off the Chrolis, push the ON/OFF button on the Chrolis front panel . This will turn off
the instrument including the LEDs. To turn off the main power supply, use the switch on the
Chrolis back panel .
Note
When switching off the LEDs in the Chrolis software there is a faint afterglow which may last
several seconds until the LEDs are completely off.
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© 2020 Thorlabs
4 Maintenance and Service
11
4 Maintenance and Service
Protect the Chrolis from adverse weather conditions. The Chrolis is not water resistant.
Attention
To avoid damage to the instrument, do not expose it to spray, liquids or solvents! Please
use a dry, lint free cloth to clean the outside or pressured air to gently blow off any
debris or dust from the inside of the Chrolis.
The unit does not need a regular maintenance by the user. It does not contain any modules
and/or components that could be repaired by the user. If a malfunction occurs, please contact
Thorlabs for return instructions.
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Chrolis
5 Appendix
5.1 Technical Data Chrolis
General Technical Specifications for CHROLIS, CHROLIS-C1 and CHROLIS-C2
CHROLIS Specifications1
Output Coupling
3 mm Liquid Light Guide (LLG03-4H included)
Compatible Bandpass Filter Size
Ø1" or Ø25 mm, Thickness: up to 0.248" or 6.3 mm
PC Interface
Full-Speed USB 2.0 (12 Mbps) (Cable Included)
Internal Modulation (square wave only)
Typ. Rise/Fall Time (10-90%)
2 µs
On Time of LED Pulse (Min to Max)
5 µs to 71 min
Off Time of LED Pulse (Min to Max)
5 µs to 71 min
Range of Number of Pulses
1 to 4.2x109or infinite
Connector for BNC Breakout Box
External Modulation (TTL)
Typ. Rise/Fall Time (10-90%)
2 µs
Typ. Trigger Delay
1 µs
LED Input Connectors
Input Impedance
2 kW(Internal Pullup to 5 V)
Max Input Voltage
5 V
Low Voltage
0 to 0.8 V
High Voltage
2.0 to 5.0 V
AUX Connectors
Impedance
50 W
Max Input Voltage
5 V
Input Low Voltage
0 to 0.8 V
Input High Voltage
2.0 to 5.0 V
Output Voltage
TTL Level
Max Output Current
25 mA
General
Power Supply Voltage
100 V -120 V / 200 V -240 V
50-60 Hz
Operating Temperature Range 2
0 - 35 °C
Storage Temperature Range
-40 to 70 °C
Dimensions (W x H x D)
11.72 " x 5.12 " x 9.90 "
(297.7 mm x 130.0 mm x 251.5 mm)
Weight
7.7 kg
1) non-condensing
2) The upper temperature is limited by the Liquid Light Guide.

© 2020 Thorlabs
5 Appendix
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5.2 Technical Data CHROLIS-C1
Technical Specifications for LEDs in CHROLIS-C1
LED Position
Nominal Wavelength (nm)
Typ. Output Power (mW)1
LED1
365
930
LED2
405
830
LED3
475
530
LED4
565
330
LED5
625
510
LED6
780
50
1) Typical output power without bandpass filter from the Liquid Light Guide LLG03-4H for configuration CHROLIS-
C1.
Dichroic Mirrors
Dichroic Mirror Position
Cut-on wavelength (nm)
D1
397
D2
458
D3
506
D4
605
D5
735
All technical data are valid at 23 ± 5°C and 45 ± 15% rel. humidity (non condensing). The Chrolis is to be used in-
doors only.

© 2020 Thorlabs14
Chrolis
5.3 Technical Data CHROLIS-C2
LEDs
LED Position
Nominal Wavelength (nm)
Typ. Output Power (mW)1
LED1
385
1100
LED2
420
700
LED3
475
450
LED4
520
170
LED5
590
100
LED6
625
580
1) Typical output power without bandpass filter from the Liquid Light Guide LLG03-4H for configuration CHROLIS-
C2.
Dichroic Mirrors
Dichroic Mirror Position
Cut-on wavelength (nm)
D1
397
D2
458
D3
506
D4
562
D5
605
All technical data are valid at 23 ± 5°C and 45 ± 15% rel. humidity (non condensing). The Chrolis is to be used in-
doors only.

© 2020 Thorlabs
5 Appendix
15
5.4 Technical Data CHROLIS
For the configurable CHROLIS, all specifications under 5.1 are valid. The technical data for the
configured LEDs and dichroic mirrors are listed below and on the Thorlabs website.
5.5 Chrolis LEDs and Dichroic Mirrors
The following table lists the available LED modules and their specifications for a customer con-
figured Chrolis:
LED Specifications
Nominal
Wavelength1
Peak Wavelength (nm)
Color
Output Power2
(mW)
Bandwidth
(FWHM)
(nm)
Lifetime3
(h)
Min
Typical
Max
365
360
365
370
UV
1130
9
>1 000
385
380
385
390
UV
1250
11
>1 000
405
400
405
410
UV
900
12
>1 000
420
415
420
425
Violet
710
14
>10 000
475
465
475
485
Blue
630
23
>10 000
490
480
490
500
Blue
120
23
>1 000
525
510
520
530
Green
180
38
>10 000
565
560
565
570
Lime
350
100
>10 000
590
590
595
605
Amber
140
80
>10 000
625
625
633
640
Red
490
13
>10 000
780
770
780
790
IR
40
28
>10 000
1) Please use the Nominal Wavelength when requesting LEDs for custom configurations.
2) Typical output power from LLG03-4H. Values can vary depending on CHROLIS configuration.
3) Typical value
Spectra of LEDs for CHROLIS
Please also see the Spec Tab on the Chrolis website for specifications of the LEDs and di-
chroic mirrors.

© 2020 Thorlabs16
Chrolis
5.6 Safety
Attention
The safety of any system incorporating the equipment is the responsibility of the assembler of
the system.
All statements regarding safety of operation and technical data in this instruction manual will
only apply when the unit is operated correctly as it was designed for.
The Chrolis must not be operated in explosion endangered environments!
Do not position the device such that operation of either On/Off switches is difficult.
Do not obstruct the air ventilation slots in the housing!
This precision device is only serviceable if returned and properly packed into the complete ori-
ginal packaging. If necessary, ask for replacement packaging.
Refer servicing to qualified personnel!
Only use the device in closed rooms.
Changes to this device can only be made using components supplied by Thorlabs and spe-
cified in chapter “Ordering Codes and Accessories”. The use of alternative components not
supplied by Thorlabs is not permitted.
This device does not contain any poisonous or injurious substances that may be liberated from
the equipment. For more information, please see the RoHS statement.
The CHROLIS housing will not overheat. Heat generating modules are temperature controlled
such that the device shuts off prior to overheating.
Please note that this product is not suitable for household room illumination.
Attention
The mains cable may not be connected to inadequate mains lines according to the specifica-
tions in Technical Data . Prior to applying power to the Chrolis, make sure that the protective
conductor of the 3 conductor mains power cord is correctly connected to the protective earth
ground contact of the socket outlet! Improper grounding can cause electric shock resulting in
damage to your health or even death!
All modules must only be operated with the supplied duly shielded connection cables.
Attention
The following statement applies to the products covered in this manual, unless otherwise spe-
cified herein. The statement for other products will appear in the respective accompanying doc-
umentation.
Note This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules and meets all requirements of the Canadian Inter-
ference-Causing Equipment Standard ICES-003 for digital apparatus. These limits are de-
signed to provide reasonable protection against harmful interference when the equipment is op-
erated in a commercial environment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a residen-
tial area is likely to cause harmful interference in which case the user will be required to correct
the interference at his own expense.
Users that change or modify the product described in this manual in a way not expressly ap-
proved by Thorlabs (party responsible for compliance) may impair the protection provided by
the equipment and the user could void the user’s authority to operate the equipment.
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