UVP BioSpectrum Imaging System Manual

BioSpectrum®Imaging System™
Instruction Guide
______________________________________________________________________________
UVP, LLC
2066 W. 11th Street, Upland, CA 91786
Tel: [phone]
Fax: [phone]
Web Site: www.uvp.com
Ultra-Violet Products Ltd.
Unit 1, Trinity Hall Farm Estate
Nuffield Road, Cambridge CB4 1TG UK
Tel: [phone] Fax: [phone]
81-0346-01 Rev F

BioSpectrum Imaging System 2
Table of Contents
Introduction..................................................................................................................................................................3
Components.................................................................................................................................................................4
Darkroom Cabinet.....................................................................................................................................................5
Five Position Filter Wheel..........................................................................................................................................5
CCD Camera and Lens.............................................................................................................................................5
UV-Safe Gel Viewer Window.....................................................................................................................................5
Lift Platform...............................................................................................................................................................5
Epi Illumination..........................................................................................................................................................6
LED White Light Illuminator.......................................................................................................................................6
Chemi Tray................................................................................................................................................................6
UV Transilluminator...................................................................................................................................................6
VisionWorks®LS Software.........................................................................................................................................7
BioLite Access Ports .................................................................................................................................................7
Installation....................................................................................................................................................................8
Installing VisionWorksLS Software............................................................................................................................8
Registering the Software...........................................................................................................................................8
Hardware Installation.................................................................................................................................................9
Camera Installation....................................................................................................................................................13
GelCam 310 Camera ..............................................................................................................................................13
MegaCam 810, OptiChemi 610 and BioChemi 510 Cameras.................................................................................15
Operation....................................................................................................................................................................17
Using the System....................................................................................................................................................17
Capturing Images.......................................................................................................................................................20
Focusing the Lens Using Image Preview................................................................................................................20
Capturing Images....................................................................................................................................................20
Service Procedures....................................................................................................................................................21
Return Procedure....................................................................................................................................................21
Troubleshooting.......................................................................................................................................................21
Replacement Parts and Accessories.......................................................................................................................21
Technical Support ...................................................................................................................................................22

BioSpectrum Imaging System 3
Introduction
The BioSpectrum Imaging System is designed to automate research with one-touch preset or user-defined PC
controls for accurate, repeatable imaging and analysis of chemiluminescence, fluorescence, bioluminescence and
colorimetric samples (depending upon configuration). The BioSpectrum incorporates a light-tight darkroom with
VisionWorksLS software for automated control.
The darkroom has a UV-blocking window, built-in overhead 365nm UV, Visi-Blue™ 480nm and white light, UV
transilluminator, LED White Light Illuminator, chemiluminescence tray and five-position emission filter wheel with
three emission filters included. The Automated BioSpectrum includes a software-controlled lift platform, while the
Manual BioSpectrum includes a manually adjustable lift platform.
The BioSpectrum includes a highly sensitive CCD camera which supplies real-time, live preview images.
Automated and Manual BioSpectrum Imaging Systems

BioSpectrum Imaging System 4
Components
The BioSpectrum Imaging System is comprised of the following equipment:
Darkroom Cabinet
Automated Filter Wheel and Emission Filters (3 Included with System)
CCD Camera and Lens:
oMegaCam 810 with either 50mm f/1.2 Fixed, 30mm f/1.4 Fixed, or f/2.8 Zoom Lens
oOptiChemi™ 610 with either 50mm f/1.2 Fixed, 30mm f/1.4 Fixed, or f/2.8 Zoom Lens
oBioChemi™ 510 with 12.5-75mm f/1.2 Zoom Lens
oGelCam 310 with 12.5-75mm f/1.2 Zoom Lens
UV-Blocking Gel Viewer Window in Darkroom Door
Automated or Manual Lift Platform (depending upon configuration)
Epi Illumination
Chemi Tray
LED White Light Illuminator
UV Transilluminator
VisionWorks®LS Software
Refer to the packing slip for a complete equipment list. System components may vary. The system may
additionally include a computer and monitor, printer or BioLite™ MultiSpectral Light Source.
CCD camera and lens are
housed under the camera cover
Five position filter wheel with
Ethidium Bromide, SYBR Green
and SYBR Gold filters included
Epi illumination with white light,
480nm and 365nm UV
Access ports for optional
BioLite MultiSpectral Light
Source
Gel viewer
window blocks
UV and allows
sample viewing
with darkroom
door closed
Light tight
darkroom with
wide access
door and UV
safety switch
LED White Light Illuminator
UV transilluminator
roll-out tray
Height-adjustable lift
platform (manual or
software-controlled
motorized)
Chemi tray (not shown)
VisionWorksLS software
controls darkroom functions,
image capture and analysis
Power indicator lights

BioSpectrum Imaging System 5
Darkroom Cabinet
The BioSpectrum Imaging System is constructed of aluminum and is
fabricated to provide a light-tight chamber.
Darkroom Dimensions: 17.5W x 17.5D x 32H inches (445 x 445 x 813
mm)
Note: The camera cover will add additional height which will vary based
on camera application.
Five Position Filter Wheel
The filter wheel accommodates up to five emission filters. The system
comes standard with Ethidium Bromide, SYBR Green and SYBR Gold
filters. Software controls the selection of filters.
CCD Camera and Lens
The high sensitivity CCD camera and fast optics, housed in the top of
darkroom, generate high resolution images. The camera and motorized
lens are controlled by VisionWorksLS software.
Refer to the packing slip for the camera configured with your system
(Note: camera shown to the right may appear different).
Refer to the Cameras section of this manual for installation and
operation instructions for the camera shipped with your system.
UV-Safe Gel Viewer Window
The viewer in the darkroom door opens for UV-safe viewing of samples.
The acrylic window allows viewing without exposure to ultraviolet
radiation.
Lift Platform
The motorized lift platform in the Automated BioSpectrum, controlled
by the software, can be finely adjusted within a 10-inch range.
The manual lift platform in the Manual BioSpectrum can be manually
adjusted to six positions within the travel range.

BioSpectrum Imaging System 6
Epi Illumination
Standard overhead lighting includes 365nm UV, Visi-Blue 480nm and
white light. Lighting is controlled via the software interface.
NOTE: The BioSpectrum includes an interlock switch that turns off all
UV illumination, including the epi 365nm UV, when the darkroom door is
opened. Be sure the darkroom door is completely closed for proper UV
operation.
LED White Light Illuminator
The LED White Light Illuminator emits high uniformity with less than 5%
coefficient of variance (CV). Store the White Light Illuminator in the
exterior darkroom side pocket when not in use.
Chemi Tray
The chemi tray creates a dark background for placement of
bioluminescent or chemiluminescent samples such as Western blots,
shown here placed on top of the FirstLight®UV Illuminator.
UV Transilluminator
The system may be configured with a UV transilluminator to fluoresce
samples. The transilluminator is positioned on a roll out tray for easy
access.
NOTE: The BioSpectrum includes an interlock switch that turns off all
UV illumination, including the transilluminator, when the darkroom door
is opened. Be sure the darkroom door is completely closed for proper
UV operation.

BioSpectrum Imaging System 7
VisionWorks
®
LS Software
VisionWorksLS software controls the darkroom functions and lighting as
well as the motorized lens and camera. The system may be configured
with VisionWorksLS analysis software for analysis of gels, plates, blots
and other samples.
BioLite Access Ports
Additional optional equipment can be added to the BioSpectrum Imaging
System through the side access ports.
Side access ports allow for connecting the external BioLite
MultiSpectral Light Source for directed lighting ranging from visible
to near IR (note: the Automated BioSpectrum has two access
ports while the Manual BioSpectrum has one access port).
The access panel on the back of the darkroom provides access for
connecting additional equipment within the darkroom.
Minimum Computer Requirements
Processor: Pentium 4, 1.6 GHz or higher
RAM: 2GB (4GB Preferred)
Display: 1024 x 768
Operating System: Microsoft Windows XP Service Pack 2 or Newer (32-bit or 64-bit)
Internet Browser: Microsoft Internet Explorer 6.0 or later
Computer USB Ports: Computer must come equipped with a minimum of three USB ports; additional
peripherals (mouse, keyboard, printer, etc.) may require additional USB ports.

BioSpectrum Imaging System 8
Installation
Installing VisionWorksLS Software
1. Insert the VisionWorksLS CD (not network CD) into the computer.
2. Click on the Install button for VisionWorksLS.
3. Click OK, Next, agree to “I accept terms of licensing agreement”, then Next. Leave all options in
their default settings. Then click Next, Next, Install and finally Finish.
Registering the Software
Open the Software
1. Double click the VisionWorksLS
software icon on the desktop.
2. To activate the software,
registration is required. To
immediately activate the software
online, choose On-the-Fly
activation. If the computer is not
connected to the Internet, select
Offline activation and proceed to
the following page of this manual,
or call UVP to register the
software.
3. Click Next to continue.
4. The Already have an activation
ID option is useful when reloading
the software after receiving an
initial activation code.
5. Complete all required information
on the form.
6. Fill out the Serial Number located
on the CD. The number should be
four sets of six numbers.
7. Once the form is completed, click
on Get Activation No. and then
click Activate once the Activation
Number appears in the box.

BioSpectrum Imaging System 9
8. If the computer is not connected to
the Internet, click Offline
activation to register the
software.
This allows the user to obtain the
activation code and enter it at
another time.
9. Click Next to continue.
10. Click the link provided and
complete the form to obtain
registration instructions. Click
Finish.
Hardware Installation
Darkroom Setup
1. Remove all the tape on the darkroom (filter wheel, doors and interior cables).
2. Detach the epi light covers from the Velcro strips at the top of the darkroom and place the covers in
a secure location.
3. Place the UV transilluminator on the pull-out tray and connect the power jumper cable to the interior
of the darkroom and to the back of the transilluminator. See “UV Transilluminator Setup” for more
information.
4. Place the chemi tray on top of the UV transilluminator. Remove the tray when not required.
5. Unpack the LED White Light Illuminator and place it on top of the UV transilluminator. Place the
LED White Light Illuminator in the external darkroom side pocket when not in use. See “LED White
Light Illuminator Setup” for more information.
6. Use a level to ensure that the darkroom is flat. If necessary, make adjustments to the four feet of
the darkroom by rotating the system’s rubber feet clockwise or counterclockwise to raise or lower
each corner of the system.

BioSpectrum Imaging System 10
Placing Filter at the Top of the
Darkroom
Darkroom Filter Setup
1. Ensure that the darkroom is in a fixed and permanent
location as filters may slide in movement.
2. Locate the filter wheel in the darkroom’s camera well at
the top of the darkroom, accessible when the camera and
lens are removed.
3. Using your fingers, manually rotate the filter wheel to the
appropriate filter location. Each filter location in the
darkroom is numbered for a corresponding filter. By
touching the sides of the filter only, insert the filter into the
proper location (the numbers/letters on the side of the filter
should face right side up).
Position #1 = Clear (no filter)
Position #2 = User Choice (filter provided if
ordered separately)
Position #3 = SYBR Green (515-570nm)
Position #4 = SYBR Gold (485-655nm)
Position #5 = Ethidium Bromide (570-640nm)
Camera and Lens Assembly
Camera Setup
There are several camera options. Detailed camera and bracket
assembly instructions for each camera are listed in the Camera
Installation section of this manual. Proceed to this section now to
continue BioSpectrum installation.
Sample Camera with Base and
Lens on Top of the Darkroom
Fixed Lens Controller Setup
The fixed lens controller is attached to the
lens at the factory. There are several
cables extending externally from the
controller. Assemble the lens controller’s
connective devices as follows:
1. Ensure that the lens controller
components and connections are
assembled per the picture at right.
2. Plug the power cable into the power
supply.
3. Plug the other end of the power
cable into an electrical outlet.
4. Wait until VisionWorksLS software
is loaded to plug the USB cable into
the computer.
Fixed lens controller and accompanying connective devices
Lens Controller
(pictured without
camera and lens)
Power Cable
Control Box
Power Supply
USB cable