UVP GelDoc-It2 Guide

GelDoc-It®2& ChemiDoc-It®2Imagers
Installation and User Instructions
UVP, LLC Ultra-Violet Products Ltd.
2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]
Web Site: www.uvp.com
81-0220-01 Rev K

GelDoc-It2and ChemiDoc-It2Imagers 2
Table of Contents
Table of Contents.........................................................................................................................................................2
Introduction..................................................................................................................................................................3
System Components ...................................................................................................................................................4
Optional Equipment...................................................................................................................................................6
Setup Instructions........................................................................................................................................................8
Components..............................................................................................................................................................8
Camera Setup...........................................................................................................................................................9
GelCam 310 Camera ................................................................................................................................................9
MegaCam 810, OptiChemi™ 610 and Chemi 415 Cameras...................................................................................11
Using the System.......................................................................................................................................................15
Powering Up the System.........................................................................................................................................15
Using the Filters ......................................................................................................................................................15
Using the Transilluminator.......................................................................................................................................16
Using the Overhead (Epi) Lighting ..........................................................................................................................16
Using the UV Gel Viewer Window...........................................................................................................................16
Capturing Images and Using Templates..................................................................................................................17
Image Focusing.......................................................................................................................................................17
Using the Preset Capture Buttons...........................................................................................................................17
Capturing Images Without Using the Preset Capture Buttons.................................................................................18
Using Templates .....................................................................................................................................................18
Service Procedures....................................................................................................................................................20
Return Procedure....................................................................................................................................................20
Replacement Parts and Accessories.......................................................................................................................20
Troubleshooting.......................................................................................................................................................20
Care and Cleaning ..................................................................................................................................................21
Technical Support ...................................................................................................................................................21
Warranty..................................................................................................................................................................22

GelDoc-It2and ChemiDoc-It2Imagers 3
Introduction
The GelDoc-It2Imager is designed as a high resolution imager capable of capturing, documenting and
analyzing fluorescent gel images.
In addition to gel documentation capabilities, the ChemiDoc-It2Imager is equipped with a cooled, scientific-
grade CCD camera allowing users to capture, document and analyze chemiluminescent blot images,
including Western blots.
The GelDoc-It2and ChemiDoc-It2Imagers incorporate a light-tight darkroom with VisionWorks®LS software
for image acquisition and analysis functions. The darkroom has a UV-blocking gel viewer window, built-in
overhead epi white lighting, a UV transilluminator and a four-position emission filter wheel with an ethidium
bromide (EtBr) emission filter included as standard.

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System Components
Refer to the packing slip and pictured components for parts included with the system.
Minimum Computer Requirements
Processor:
Pentium class, 1.6 GHz or higher
RAM:
1 GB (2GB Preferred)
Display:
1024 x 768
Operating System:
Microsoft Windows XP Service Pack 2 or Newer (32-bit or 64-bit)
Internet Browser:
Microsoft Internet Explorer 6.0 or later
Computer USB Ports:
Computer must come equipped with a minimum of three USB
ports; additional peripherals (mouse, keyboard, printer, etc.) may
require additional USB ports.
Camera and Lens Kit
(actual configuration may
be different than that
shown in the photo)
Lighting and UV
Timer Switches
Darkroom
Access Door
Epi Overhead
White Light
Four Position
Emission Filter
Selector
UV Safety
Interlock Switch
UV-Safe Gel
Viewer
Window
Roll-Out
Transilluminator
LED White Light
Plate (Optional)

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Cameras
GelDoc-It2: The GelDoc-It2is equipped with the GelCam 310 camera, a scientific-grade monochrome
CCD camera with a resolution of 2.0MP (1600x1200) with USB 2.0 PC interface.
ChemiDoc-It2: The ChemiDoc-It2 is equipped with the Chemi 415, OptiChemi™ 610 or MegaCam 810
scientific-grade monochrome CCD camera. All cameras are Peltier cooled and offer full 16-bit file bit
depth:
The Chemi 415 camera has 1.4MP (1360x1024) resolution with Peltier cooling to 35°C below
ambient. The Chemi 415 has a peak quantum efficiency of 62% and is capable of binning from 1x1
to 8x8.
The OptiChemi 610 camera has 3.2MP (2184x1472) resolution with Peltier cooling to 50°C below
ambient. The OptiChemi 610 has a peak quantum efficiency of 86% and is capable of binning from
1x1 to 10x10.
The MegaCam 810 camera has 8.1 MP (3296x2472) resolution with Peltier cooling to 35°C below
ambient. The MegaCam 810 has a peak quantum efficiency of 50% and is also capable of binning
from 1x1 to 8x8.
All camera settings are factory pre-set for optimum performance when viewing gels, films or
membranes under low light level conditions. Contact UVP Technical Support prior to making any
adjustments to the camera settings.
Lens Kit (Motorized or Manual)
The zoom lens used with the GelCam 310 and Chemi 415 cameras comes in two configurations:
The motorized lens is controlled via VisionWorksLS Software.
The manual lens requires manual user adjustment.
Some lenses may be fitted with a close-up diopter. The diopter is used for focusing on objects at the
focal length of the GelDoc-It2/ChemiDoc-It2darkroom.
Ethidium Bromide (EtBr) Emission Filter
The ethidium bromide (50mmsquare) UV-blocking bandpass interference filter blocks UV and IR
radiation emitted from the transilluminator. The filter is placed in the filter wheel below the camera
assembly. The filter allows visualization of fluorophores from 580-630nm, targeting the ethidium
bromide emission peak of 605nm.
Additional filters are available for other specific fluorophores. Filters can also be removed when imaging
non-fluorescent media (including chemiluminescent blots, protein gels, colony plates, etc.) in order to
produce brighter images. Contact UVP for ordering information.
Darkroom
The darkroom is light tight to provide optimal imaging conditions. Darkroom features include:
Overhead white light
Roll-out transilluminator tray
UV-safe gel viewer window built into the darkroom door
Side pocket for storage of materials
Four position emission filter wheel
Brackets for installing optional UV handheld lamps for epi (overhead) UV illumination
Built-in power ports for transilluminator and optional overhead UV handheld lamps
Built-in power cord for optional LED White Light Plate
UV safety interlock switch with manual override to disable UV transillumination and
optional UV epi illumination when darkroom door is opened

GelDoc-It2and ChemiDoc-It2Imagers 6
Thermal Printer
UV Lamp
Fluorescent Focus Target
Transilluminator
The GelDoc-It2and ChemiDoc-It2Imagers can accommodate UVP’s Benchtop and FirstLight
transilluminator models. UVP offers a variety of transilluminator configurations, including Benchtop
models with multiple wavelengths and variable intensities as well as the highly uniform, patented
FirstLight®transilluminator. Refer to the packing slip for the transilluminator included with your system.
Note: For UV protection and to extend the life of the UV transilluminator, the system incorporates a ten-
minute transilluminator shutoff timer. The timer shuts off the transilluminator after ten minutes of
operation. A push button on the front of the darkroom allows the user to reset the timer. Alternately, the
timer can also be reset by opening and closing the darkroom door.
Fluorescent Focus Target
The UVP Fluorescent Focus Target fluoresces when placed
on a UV transilluminator or when exposed to overhead UV.
The Target provides sharp fluorescent images to aid in
adjusting the lens and camera settings for ideal imaging
results.
Optional Equipment
UVP offers a variety of optional equipment to support the needs of varying laboratory environments. Refer to
“Replacement Parts and Accessories” at the end of this manual for optional equipment part numbers.
Thermal Printer
The thermal printer provides archive quality, 256 grayscale
prints and five optional cost-effective print sizes.
UV Handheld Lamps for Overhead Lighting
Two 4-watt ultraviolet lamps can be connected inside the
darkroom to provide epi UV illumination. These lamps can be
switched on or off using the switch on the front of the
darkroom and can also be removed from the darkroom and
used as standalone handheld lamps (as seen in the image to
the right).

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UV/White Converter Plate
LED White Light Plate
LED White Light Plate
The LED White Light Plate emits high uniformity with less
than 5% coefficient of variance (CV). Plug the LED White
Light Plate directly into the power supply within the system
darkroom, or store it in the exterior darkroom side pocket
when not in use.
Converter Plates
An alternate to the LED White Light Plate, the UV/White
Converter Plate allows imaging of non-fluorescent stained
media with an ultraviolet transilluminator. The converter plate
is specially coated to convert 302nm UV output to white light
rather than using a separate white light box or plate.
The Visi-Blue Converter Plate (not shown) converts UV to a
safe 460-470nm blue wavelength designed for use with blue
excitation samples and SYBR Green, SYPRO Orange and
GFP stains.

GelDoc-It2and ChemiDoc-It2Imagers 8
Setup Instructions
Components
When unpacking the GelDoc-It2and ChemiDoc-It2, the following items will be included:
GelDoc-It2 or ChemiDoc-It2darkroom
Transilluminator
Camera with lens and bracket
Ethidium Bromide (EtBr) emission filter
Electrical and computer cables
VisionWorksLS Software
WARNING: Do not attempt to perform any setup procedures while the system is plugged in or powered on
unless otherwise instructed.
CAUTION: Do not install the system in areas with high moisture, dust or high temperatures. Keep the
equipment away from motors or any other large magnetic equipment apparatus.
Connecting the Power Cables
1. Inside the darkroom, place the transilluminator on the roll-out tray. Connect the transilluminator to
one end of the jumper cable. Connect the other end of the jumper cable to the interior of the
darkroom. Turn the UV transilluminator’s main power switch (green switch) ON.
2. If installing the LED White Light Plate, place the plate on top of the UV transilluminator and connect
the power cord coming from inside the darkroom to the back of the Plate.
3. If installing epi UV handheld lamps, place the lamps in the brackets located at the top of the
darkroom. Plug the handheld lamps into the outlets provided inside the darkroom. Place the power
switches located on the outside of the handheld lamps in the ON position.
4. Connect the main power cord from the back of the darkroom to a surge-protected power outlet.
Installing the Emission Filter
To install the 50mm2ethidium bromide (EtBr) filter:
1. Carefully remove filter from the protective
plastic case, holding the filter at the edges to
prevent placing fingerprints on the glass
surface.
Note: It may be necessary to remove the base
and camera assembly to insert new filters
(photograph shows camera and base removed).
2. The filter wheel is located inside the top of the
darkroom. Before placing the filter in the filter
wheel, ensure that the text on the edge of the
filter is positioned so it is right side up when
facing the installer.
3. Place the filter in the filter wheel.
4. Write the name of the filter on the appropriate
label at the front of the darkroom.

GelDoc-It2and ChemiDoc-It2Imagers 9
Additional and replacement emission filters are available through UVP. Refer to the Replacement
Parts and Accessories section of this manual for ordering information.
Camera Setup
High sensitivity, scientific-grade CCD cameras are designed for use with UVP’s GelDoc-It2and ChemiDoc-it2
Imagers. This section covers the components and steps required to UVP’s cameras. The following cameras
are covered in this manual (refer to the packing list for the camera included with your specific system):
GelCam 310 (GelDoc-It2)
Chemi 415 (ChemiDoc-It2)
OptiChemi 610 (ChemiDoc-It2)
MegaCam 810 (ChemiDoc-It2)
GelCam 310 Camera
Specifications GelCam 310
Type CCD, Monochrome
Resolution 1600 x 1200
Bit Depth 16-bit
PC Interface USB
GelCam 310 Camera with Manual Lens
The GelCam 310 camera, zoom lens and diopter
are assembled at the UVP factory.
1. Remove the cap from the lens (if in place).
2. Using the four brass thumb screws provided,
secure the bracket to the base on the
darkroom.
3. Slide the camera and lens assembly into the
camera bracket. Slide the lens through the
center hole in the black rubber gasket. The
gasket forms a seal around the lens.
4. Insert and tighten the black thumb screw in
the central hole of the camera to secure the
camera to the bracket.
5. Plug the camera cable into the top of the
camera but do not plug the other end of the
USB cable into the computer until
VisionWorksLS software has been installed.
Tighten brass thumb
screws to secure the
bracket to the base
Camera and
manual lens
assembly
Black thumb
screw

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GelCam 310 Camera with Motorized Lens
The GelCam 310 camera, zoom lens and diopter
are assembled at the UVP factory.
1. Remove the cap from the lens (if in place).
2. Using the four brass thumb screws provided,
secure the bracket to the base of the
darkroom.
3. Slide the camera and lens assembly into the
camera bracket.
4. Insert and tighten the black thumb screw in
the central hole of the camera to secure the
camera to the bracket.
5. Plug the camera cable into the top of the
camera but do not plug in the other end of the
USB cable until VisionWorksLS software has
been installed.
6. Mount the lens control box on the hook
located at the back of the darkroom.
Tighten brass
thumb screws to
secure the bracket
to the base
Camera cable
Power switch for the Lens
Control Box
Central hole
Black thumb
screw