VWR avantor PCR User manual

Instruction manual
VWR®PCR Workstation
EU cat. no 732-2541
NA cat. no 10783-132
UK cat. No 732-2542
___
Version 02 Issue 08/2021

2vwr.com I Instruction manual VWR®PCR Workstation
Legal address of Manufacturer
Europe
VWR International bv,
Researchpark Haasrode 2020
Geldenaaksebaan 464 B-
3001 Leuven
[phone]
be.vwr.com
UK Importer:
VWR International Ltd
Hunter Boulevard, Magna Park
Lutterworth, Leicestershire, LE17 4XN
uk.vwr.com
United States
VWR International LLC
100 Matsonford Rd
Radnor, PA 19087
[phone]
www.vwr.com
Country of Origin: GERMANY

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Table of Contents
Introduction................................................................................................................................................................................................................4
Delivery contents.....................................................................................................................................................................................................4
Safety INSTRUCTIONS .........................................................................................................................................................................................5
ASSEMBLING the PCR Workstation............................................................................................................................................................... 7
Step 1.................................................................................................................................................................................................. 7
Step 2 ................................................................................................................................................................................................. 8
Step 3 ................................................................................................................................................................................................. 9
Step 4.................................................................................................................................................................................................9
Step 5................................................................................................................................................................................................. 9
Specifications.......................................................................................................................................................................................... 10
VWR PCR Workstation.......................................................................................................................................................................................10
Power supply ...........................................................................................................................................................................................................10
Connections ..............................................................................................................................................................................................................11
AC power inlet................................................................................................................................................................................ 11
AC power outlets ...........................................................................................................................................................................12
Operating the PCR Workstation.................................................................................................................................................................... 13
Use of PCR equipment inside the workstation......................................................................................................................13
Operating the overhead UV and white lights .......................................................................................................................13
Operating the UV Air Recirculator............................................................................................................................................13
Timer ................................................................................................................................................................................................ 14
Control panel description.................................................................................................................................................................................. 15
Select day and time..................................................................................................................................................................... 16
Software menu .............................................................................................................................................................................. 16
Set up UV Surface time............................................................................................................................................................... 16
Set up Auto Decontamination routine.....................................................................................................................................17
Checking and resetting the hours of duty............................................................................................................................. 19
Settings............................................................................................................................................................................................20
Measuring UV tube intensity............................................................................................................................................................................22
Bacterial destruction chart...............................................................................................................................................................................23
Maintenance ...........................................................................................................................................................................................24
Care and cleaning........................................................................................................................................................................24
Replacing the UV germicidal or white light tubes...............................................................................................................25
Replacing the UV Air Recirculator UV tube .......................................................................................................................... 25
Replacing the fuse........................................................................................................................................................................25
Replacing the filter of the UV Air Recirculator.....................................................................................................................25
Ordering Information...........................................................................................................................................................................................26
Technical service....................................................................................................................................................................................26
Web resources...............................................................................................................................................................................26
Warranty....................................................................................................................................................................................................................26
Compliance with local laws and regulations...........................................................................................................................................27
Disposal Instruction............................................................................................................................................................................... 27
Our local offices in Europe, AMEA and North America ......................................................................................................................28

4vwr.com I Instruction manual VWR®PCR Workstation
Introduction
The VWR PCR Workstation is designed as an ideal environment for the manipulation of DNA and
RNA, especially for the set-up of PCR assays. Contamination can lead to false or misleading results
which costs time and money. The VWR PCR Workstation minimises the risk of contamination. It
provides a 'separate room', e.g. for the set-up of PCR reactions.
The high intensity surface UV tubes inactivate DNA as a source of contamination between
experiments. In addition, a UV Air Recirculator is integrated into the workstation system which
reduces airborne contaminants during experiments. UV light is effectively blocked by solid
polycarbonate screens to ensure maximum protection for the user.
The surface of the workstation is constructed of stainless steel, which is very robust and can easily
be cleaned plus has an antimicrobial effect.
The VWR PCR Workstation offers a controlled environment for PCR and RNA applications that
protects your samples and helps to achieve optimal results.
Delivery contents
After receipt of the system, please check if the following items are included in the shipment:
Base (1x)
Rear panel (1x)
Side panel (2x)

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Front panel (1x)
Shelf (2x)
Top assembly (1x)
Ventilation cover (1x)
Screw (31x)
Screwdriver (1x)
Main cable (1x)
Safety INSTRUCTIONS
The PCR Workstation is designed with function, reliability, and safety in mind.
Before using the PCR Workstation please read this entire manual and strictly follow the safety
instructions!
Caution: The unit includes shortwave UV, which is a powerful source of UV radiation that will cause

6vwr.com I Instruction manual VWR®PCR Workstation
damage to unprotected eyes and skin if directly exposed to the UV lamps. Before operating any
unit, ensure that the PCR Workstation is not damaged, and that instructions for use of this
equipment are followed. A safety shut-off switch automatically turns the UV light off when the
front panel is open, protecting users from UV exposure. The polycarbonate panels are UV-
blocking.
Intended use of the PCR Workstation: Working environment for protection of PCR reactions /
reaction set ups and genetic substances against pollution and contamination.
Safety and protection of the user can not be ensured if the PCR Workstation is used in any manner
that is not specified in these instructions. Special notice must be taken of the following:
Caution: Dangerous voltage
Ensure that the voltage requirements of the PCR Workstation
exactly match your local AC power supply.
Only use the original AC cable supplied with the PCR Workstation.
Caution: Liquids
Ensure that no liquids can enter the device. Do not place liquids on
top of the PCR Workstation.
Caution: Dangerous explosive material
Do not use reactive or explosive substances in the PCR
Workstation.
Caution: Damage
Check for transport damage and completeness of the accessories
upon arrival. If any damage is noticed, contact your distributor or
manufacturer immediately. If you detect any damage of the PCR
Workstation or one of its accessories, do not connect the PCR
Workstation to the AC wall outlet and do not use the device in any
manner!
Caution: Optical radiation
Do not switch on the PCR Workstation if not completely
assembled. See “ASSEMBLING THE PCR WORKSTATION”, page 6.
Do not look directly into the UV lamps.

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ASSEMBLING the PCR Workstation
The PCR Workstation should be assembled by two people, directly at the site of operation. We
recommend the wearing of safety gloves for hand protection.
Step 1
Put the base on an even surface with the
rubber feet facing down and the sensor cable
outlet facing to the front. Lay the back panel 
onto the base with the power outlets facing
down, and the power input and the type label
facing to the right.
Connect both sensor cables.
Slide the connection assembly into the cavity
of the back panel.
Base

Back panel


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Slot the back panel onto the base . The
connecting link of the base must fit in the
back panel . Take care not to squeeze the
sensor cable.
Connect both parts by screwing 3 screws into
the back side. Use the screw driver to do this.
Turn the parts around 90° on the site of
operation and incline both screwed parts to the
back part of the back panel . Screw both
panels with 2 screws from underneath. Tilt
back the panels in starting position with the
back panel facing backwards.
Step 2
Remove the protective foil from the side panels.
Insert the side panels into the guiding rails at
the base and push up against the back panel
within the guiding rails at both sides. Carefully
screw the side panels with 3 screws each in
the base and the back panel , respectively.
Use the supplied screws and the screwdriver
.
Back panel

Base

Side panel

Side panel


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Step 3
Put the shelves into the shelf brackets at the
back panel .
Remove the protective foil from the front panel.
Put the front panel onto the side panels .
Take care that the screw holes of the front panel
match the threaded holes in the side panels .
Hold the front panel in place. Screw the front
panel to both side panels using 3 screws 
each. Use the screwdriver .
Step 4
Turn the assembled parts around 180°.
Remove the small UV tube at the back of the top
assembly.
Put the top assembly onto the panels. Take
care not to squeeze the cables protruding from
the back panel. Screw the top assembly to the
back panel at two positions. Use the screws 
and screwdriver supplied.
Connect the sensor cables from the back panel
and the top assembly . Put the power plug
of the back panel into the power outlet of the
top assembly .
Step 5
Insert the UV tube back in place.
Cover the service access of the top assembly 
with the ventilation cover . Screw both parts
together with 6 screws . Use the screw-driver
.
Turn the system around 180° and place it at the
site of operation as needed.
Finally connect the system to the power supply
by plugging the power plug from the back
panel into the local power outlet.
The system is now ready for use.
Shelves

Front panel

Side panel

Side panel

Top assembly

Back panel

Ventilation cover

Top assembly


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Specifications
VWR PCR Workstation
Light sources integrated into the top assembly:
•2x 25 W 254 nm UV light tubes – for effective surface decontamination
•1x 8 W 254 nm UV light tube – for decontamination of air during work in UV Air
Recirculator
•1x 15 W white light tube – for a bright work surface
Features:
•Four power outlets
•Two shelves made from stainless steel
•Electronic UV timer
•Working area and back panel made from stainless steel
•Front and side panels made from polycarbonate (Makrolon®) that blocks wavelengths
below 400 nm
WxHxD:
750x780x620 mm
Weight:
48 kg
Pollution rate:
2
Environmental temperature range:
+10 to +30 °C
Maximum relative humidity:
70%
Maximum height above sea level:
2000 m
Noise level:
<51 dB (A)
Circulation rate fan: 56 m3/h
Power supply
EU/UK version: 220 - 240 V AC, 50/60 Hz, max. 1650 VA
US version: 120 V, 60 Hz, max. 900 VA
Fuse: 10 AT, 5 x 20 mm
For indoor use, only!

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Connections
AC power inlet
Before first use, ensure that the voltage requirements of the PCR Workstation exactly match your
local AC power supply. Connect the PCR Workstation to the AC power supply only by the AC
power cord delivered with your PCR Workstation. Only connect the PCR Workstation to electric
power systems with an earth conductor. The AC power inlet and the AC power switch are located
at the rear of the workstation. The AC power switch serves as a disconnection of the AC power
supply from the device. The accessibility of the AC power switch must be not hindered or obscured
in any way.
The electric fuse is placed at the back of the device between the power switch and the AC power
inlet. After pulling off the AC power cord and pushing upwards a safety catch, the fuse can be
changed. Use only a fuse with correct values (see "SPECIFICATIONS", page 10). Indications about
the fuse type are also located at the back of the device.
If a service is required, contact your local distributor or the manufacturer (see "TECHNICAL
SERVICE AND ORDER INFORMATION", page 22).
The serial number of the PCR Workstation is located at the rear of the instrument. The
replacement of single components (except polycarbonate panels, UV tubes and the UV Air
Recirculator fuse and filter) must not be done by the user but exclusively by authorised specialists
using original replacement parts. Therefore, the device must be sent to the manufacturer.
Unauthorised work on the device voids the warranty.
The surface of the device should be decontaminated with a lint-free cloth soaked with 70%
ethanol before sending. Please fill in and sign the decontamination certificate and send it to
the manufacturer together with the device.
For safety reasons the device must be switched off and the mains plug pulled before cleaning
is performed!
AC power inlet
AC power switch
Fuse

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AC power outlets
The 4 AC power outlets serve as power supplies for devices used inside the PCR chamber.
The sum of the maximum power ratings of devices connected to these power outlets
must not exceed the values specified for the PCR Workstation.
See chapter "OPERATING THE PCR WORKSTATION ", page 13, for further
information.
4x AC power outlet

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Operating the PCR Workstation
Use of PCR equipment inside the workstation
Shelves are provided for the placing of small tools for storage and decontamination.
Power outlets allow use of shakers, rockers and other equipment for PCR experiments inside the
PCR chamber. The equipment can be decontaminated between experiments. To operate
equipment within the chamber, plug the equipment into the power plug. Specific power plugs are
installed as shown in the illustration "AC power outlets", page 12.
Timing of UV decontamination can be programmed for time-controlled every day
decontamination.
NOTE: When operating, equipment plugged into the outlets, combined amp total should not
exceed 6 amps.
NOTE: Crazing is a normal process for polycarbonate panels exposed to UV light. Crazing will
occur over a period of time. Reduce crazing by keeping exposure to UV to a minimum. Crazing
may occur within the warranty period and is regarded as normal wear and tear not covered by the
warranty. Polycarbonate panels are available as spare parts and can be replaced.
Operating the overhead UV and white lights
The PCR Workstation provides an automated process for eliminating contamination by using the
decontaminating properties of shortwave 254 nm ultraviolet light. The UV light is typically
operated when no samples are inside the chamber. The chamber is decontaminated and DNA as a
possible contamination is inactivated.
The overhead white light can remain lit at any time throughout an experiment or a
decontamination process. The fluorescent white light provides a bright light within the PCR
Workstation.
Operating the UV Air Recirculator
The VWR PCR Workstation includes a built-in UV Air Recirculator for elimination of airborne
microbes during experiments.
NOTE: The UV Air Recirculator can operate while the front panel is open.
The UV Air Recirculator circulates airflow into and out of the chamber. The air is sucked into the
UV Air Recirculator in the top of the chamber, the air outlets are at the bottom of the back panel.
The drawing indicates the location of the air vents.

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NOTE: Equipment in front of the air vents will block airflow.
Timer
The default setting is 30 minutes adjustable at 5 minutes’ increments down to a minimum of 5
minutes. For information on setting the timer please see page 16/17.
Note: A magnetic sensor is built into the bottom of the front panel. When the front panel is
opened, the ultraviolet light will automatically shut off!
Note: Do not attempt to perform PCR procedures with the germicidal lamp on. The germicidal
lamp is used to decontaminate the chamber between experiments.

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Control panel description
To scroll or increase selected value
To scroll or decrease selected value
Enables to select and change the displayed program settings. Several clicks to
jump from value to value.
Enables to open and close the software menu
Switch white light on and off
Switch UV light on and off
It automatically activates the programme to decontaminate the workspace.
The default time for the decontamination process is 30 minutes.
The display will show the remaining time of the decontamination programme.
Remaining time could be change with the arrow buttons.
Switch Recirculator on and off

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Select day and time
Press from the home screen to change
day and time. Pressing the or button will
modify the selected value.
Press to go to the next values: hours,
minutes and safe.
Software menu
Press to open the software menu.
Pressing the

or

button will go through
the menu items. An arrow is displayed near
the selected item.
The items are:
•UV Surface
•Auto Decontamination
•Duty hours
•Settings
Set up UV Surface time

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Set up Auto Decontamination routine
The following parameters can be selected
Start time
Weekdays
Functions
Pressing the or button will modify from
OFF to ON. To activate or deactivate the daily
decontamination routine.

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(S = Sunday) will store the settings.
Press to go back to the
decontamination routine parameters.
Select “Functions” and press . To select
the Functions for the auto decontamination,
click on the function buttons on the right
panel. A blue light will flash if a function is
selected. To save the selection press .
Note: During Auto-Decontamination all
selected functions will be activated
automatically, after reaching the selected time
only the UV Light will be deactivated all other
functions (Recirculator) are still running to
protect the device from re-contamination.
Press to go back to the
decontamination routine parameters.
To stop a running Auto Decontamination
select Abort? Yes and press .
After finalizing an Auto Decontamination run
the final Time will be shown in the display,
press to go back to the main screen.
Note: During Auto-Decontamination all
selected functions will be activated
automatically, after reaching the selected time
only the UV Light will be deactivated. All other
selected functions (e.g. Recirculator) are still
active to protect the device from re-
contamination.

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Checking and resetting the hours of duty
UV Surface
UV Recirculator
rogress bar will indicate
when the device needs to be replaced.
We recommend to change the UV tubes after
1000 – 1500 operating hours as the UV intensity
decreases significantly after that time.
to
go back to the Duty hours selection parameters.
After replacing UV tubes it is necessary to
reset the hours of duty in the software
manually!

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Settings
Brightness
Contrast
Language
Time Format
Factory Reset
rogress bar starts flashing and indicates the
brightness of the display. Press the or 
buttons to change the value. Press
to
go back to the Settings parameters
rogress bar starts flashing and indicates the
contrast of the display. Press the or 
buttons to change the value. Press
to
go back to the Settings parameters
Press the or buttons to modify the
selected language. Languages are:
English, German, Espanola, Francis, Italian and
Portuguese. Press to confirm and store
the selected language. Press to go back
to the Settings parameters.