VWR PCR Workstation User manual

VWR®PCR Workstation
INSTRUCTION MANUAL
Catalogue Numbers:
Euro Plug: 732-2840 (assembled)
732-3337 (not assembled)
UK Plug: PEQL90-UV/PCR2-UK
Version: 1.3
Issued: 01 June 2018

VWR Collection PCR Workstation HEPA
Legal Address of Manufacturer
Unite
d States
Europe
VWR International, LLC VWR International bvba
100 Matsonford Rd Researchpark Haasrode 2020
Radnor, PA 19087 Geldenaaksebaan 464
800-932-5000 B-3001 Leuven
http://www.vwr.com + 32 16 385011
http://be.vwr.com
Country of origin Germany

VWR Collection PCR Workstation 2
CONTENTS
CONTENTS 2
INTRODUCTION 3
GENERAL WARNING 3
SAFETY INSTRUCTIONS 3
DELIVERY CONTENTS 5
UNPACKING 6
ASSEMBLING THE PCR WORKSTATION 7
SPECIFICATIONS 10
VWR PCR Workstation 10
Power supply 10
CONNECTIONS 11
AC power inlet 11
AC power outlets 12
OPERATING THE PCR WORKSTATION 13
Use of PCR equipment inside the workstation 13
Operating the overhead UV and white lights 13
Operating the UV Air Recirculator 13
Timer 14
Control panel description 15
Select day and time 16
Software menu 16
Set up UV Surface time 16
Set up Auto Decontamination routine 17
Checking and resetting the hours of duty 19
Settings 20
MEASURING UV TUBE INTENSITY 22
Bacterial destruction chart 23
MAINTENANCE 24
Care and cleaning 24
Replacing the UV germicidal or white light tubes 24
Replacing the UV Air Recirculator UV tube 25
Replacing the filter of the UV Air Recirculator 25
Replacing the fuse 25
ORDERING INFORMATION 26
TECHNICAL SERVICE 26
WARRANTY 26
COMPLIANCE WITH LOCAL LAWS AND REGULATIONS 26
DISPOSAL INSTRUCTION 27

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INTRODUCTION
The VWR PCR Workstation is designed as an ideal environment for the manipulation of DNA and
RNA, especially for the set-up of PCR assays. Contamination can lead to false or misleading results
which costs time and money. The VWR PCR Workstation minimises the risk of contamination. It
provides a 'separate room', e.g. for the set-up of PCR reactions.
The high intensity surface UV tubes inactivate DNA as a source of contamination between
experiments. In addition, a UV Air Recirculator is integrated into the workstation system which
reduces airborne contaminants during experiments. UV light is effectively blocked by solid
polycarbonate screens to ensure maximum protection for the user.
The surface of the workstation is constructed of stainless steel, which is very robust and can easily be
cleaned plus has an antimicrobial effect.
The VWR PCR Workstation offers a controlled environment for PCR and RNA applications that
protects your samples and helps to achieve optimal results.
GENERAL WARNING
The PCR Workstation has been designed for laboratory use and it should not be used at temperature
below 15 °C. If the instrument is at a temperature below 10 °C place it in a warmer room and wait at
least two hours before using it. Before plugging the instrument into a power supply, check that the
voltage and frequency on the label matches the power supply specifications. Before any operation
ensure that the ventilation grids are not obstructed and the door is properly closed.
SAFETY INSTRUCTIONS
The PCR Workstation is designed with function, reliability, and safety in mind.
Before using the PCR Workstation please read this entire manual and strictly follow the safety
instructions!
Caution: The unit includes shortwave UV, which is a powerful source of UV radiation that will cause
damage to unprotected eyes and skin if directly exposed to the UV lamps. Before operating any unit,
ensure that the PCR Workstation is not damaged, and that instructions for use of this equipment are
followed. A safety shut-off switch automatically turns the UV light off when the front panel is open,
protecting users from UV exposure. The polycarbonate panels are UV-blocking.
Intended use of the PCR Workstation: Working environment for protection of PCR reactions /
reaction set ups and genetic substances against pollution and contamination.
Safety and protection of the user cannot be ensured if the PCR Workstation is used in any manner
that is not specified in these instructions. Special notice must be taken of the following:
Caution: Dangerous voltage
Ensure that the voltage requirements of the PCR Workstation exactly match your local
AC power supply.
Only use the original AC cable supplied with the PCR Workstation.
Caution: Liquids
Ensure that no liquids can enter the device. Do not place liquids on top of the PCR
Workstation.

VWR Collection PCR Workstation 4
Caution: Dangerous explosive material
Do not use reactive or explosive substances in the PCR Workstation.
Caution: Damage
Check for transport damage and completeness of the accessories upon arrival. If any
damage is noticed, contact your distributor or manufacturer immediately. If you detect any
damage of the PCR Workstation or one of its accessories, do not connect the PCR
Workstation to the AC wall outlet and do not use the device in any manner!
Caution: Optical radiation
Do not switch on the PCR Workstation if not completely assembled. See “ASSEMBLING
THE PCR WORKSTATION”, page 7.
Do not look directly into the UV lamps.

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DELIVERY CONTENTS
After receipt of the system, please check if the following items are included in the shipment:
1. Base (1x)
2. Rear panel (1x)
3. Side panel (2x)
4. Front panel (1x)
5. Shelf (2x)
6. Top assembly (1x)

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7. Ventilation cover
(1x)
8. Screw (31x)
9. Screwdriver (1x)
10. Main cable (1x)
UNPACKING
Remove the instrument from its packaging and inspect it for signs of damage. If any are
discovered, inform your supplier immediately
The instrument must be placed on a stable, level surface and positioned such that air can
circulate freely around the casing
Ensure your proposed installation site conforms to the environmental conditions for safe
operation
The instrument is designed for indoor use only, temperature range of 10 to 35 °C and humidity
of 35 to 70% are recommended
If the instrument has just been unpacked or has been stored in a cold environment, it should
be allowed to come to room temperature for 2 – 3 hours in the laboratory before switching on.
This will prevent calibration failure because of internal condensation
The instrument must be connected to the power supply with the power cord supplied.
It is recommended that users should thoroughly read this manual prior to use
Contact your supplier if you experience any difficulties with this instrument

VWR Collection PCR Workstation 7
ASSEMBLING THE PCR WORKSTATION
The PCR Workstation should be assembled by two people, directly at the site of operation. We
recommend the wearing of safety gloves for hand protection.
Step 1
Put the base 1on an even surface with the
rubber feet facing down and the sensor
cable outlet facing to the front. Lay the back
panel 2onto the base 1with the power
outlets facing down, and the power input
and the type label facing to the right.
Connect both sensor cables.
Slide the connection assembly into the
cavity of the back panel.
Base 1
Back panel 2

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Slot the back panel 2onto the base 1. The
connecting link of the base 1must fit in the
back panel 2. Take care not to squeeze
the sensor cable.
Connect both parts by screwing 3 screws 8into the back side. Use the screw driver 9to do this.
Turn the parts around 90° on the site of operation and incline both screwed parts to the back part of
the back panel 2. Screw both panels with 2 screws 8from underneath. Tilt back the panels in
starting position with the back panel facing backwards.
Step 2
Remove the protective foil from the side
panels.
Insert the side panels 3into the guiding
rails at the base 1and push up against the
back panel 2within the guiding rails at both
sides. Carefully screw the side panels 3
with 3 screws each in the base 1and the
back panel 2, respectively. Use the
supplied screws 8and the screwdriver 9.
Step 3
Put the shelves 5into the shelf brackets at
the back panel 2.
Remove the protective foil from the front
panel.
Put the front panel 4onto the side panels
3.
Take care that the screw holes of the front
panel match the threaded holes in the side
panels 3. Hold the front panel 4in place.
Screw the front panel 4to both side panels
3using 3 screws 8each. Use the
screwdriver 9.
Back panel 2
Base 1Side panel 3
Side panel 3
Shelves 5
Front panel 4Side panel 3
Side panel 3

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Step 4
Turn the assembled parts around 180°.
Remove the small UV tube at the back of the
top assembly.
Put the top assembly 6onto the panels.
Take care not to squeeze the cables
protruding from the back panel. Screw the
top assembly to the back panel 2at two
positions. Use the screws 8and screwdriver
9supplied.
Connect the sensor cables from the back
panel 2and the top assembly 6. Put the
power plug of the back panel 2into the
power outlet of the top assembly 6.
Step 5
Insert the UV tube back in place.
Cover the service access of the top
assembly 6with the ventilation cover 7.
Screw both parts together with 6 screws 8.
Use the screw-driver 9.
Turn the system around 180° and place it at
the site of operation as needed.
Finally connect the system to the power supply by plugging the power plug from the back panel 2
into the local power outlet.
The system is now ready for operation now.
Top assembly 6
Back panel 2
Ventilation cover 7
Top assembly 6