VWR Peqlab Primus 25 advanced User manual

Instruction Manual – Bedienungsanleitung
Primus 25 advanced®
Innovative PCR Technology /
Innovative PCR-Technologie
0314E+D Creating the future
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Instruction manual Primus 25 advanced®
PEQLAB_v0314_E
1
CONTENTS
1SAFETY INSTRUCTIONS 2
2LICENSE NOTICE 2
3SYSTEM OVERVIEW 3
4INSTALLATION 4
5MAIN MENU 4
5.1 RUN 4
5.2 SELECT + RUN PROGRAM 4
5.3 RUN INCUBATION 5
5.4 MODE/EDIT/UTILS 5
5.4.1 PROGRAM 5
5.4.2 VIEW 5
5.4.3 PRINT 5
5.4.4 SETUP 5
6PROGRAMMING 6
6.1 Program steps 6
6.2 Sample program 7
7TECHNICAL SUPPORT AND ORDERING INFORMATION 8
8TECHNICAL SPECIFICATIONS 9
9MAINTENANCE AND REPAIR OF THE THERMAL CYCLER 10
9.1 Cleaning, maintenance and repair of the thermal cycler 10
9.2 Temperature check-up 10
9.3 Calibration 10
10 WARRANTY 10
11 DISPOSAL INSTRUCTION 11

Instruction manual Primus 25 advanced®
PEQLAB_v0314_E 2
1SAFETY INSTRUCTIONS
Before the first use of the thermal cycler please read the instruction manual entirely.
Special notice should be taken of the following
Caution of dangerous voltage
Please ensure the voltage indicated on the device exactly matches your local electri-
cal supply
Caution of dangerous explosive material
Explosive or reactive material mustn’t be heated in the thermal cycler.
Caution - liquids
Ensure that no liquids can enter the device.
Caution - hot surfaces
The thermal plate and the heated lid quickly reach temperatures above 50°C. There
is danger of burning! Keep the lid closed until the temperatures reaches 30°C or less.
Only use materials (tubes, tube caps, plates and sealing films) which are heat resis-
tant to 120°C
Caution – environment
The ventilation of the device must not be covered.
2LICENSE NOTICE
This instrument is licensed for research and development and for uses other than human in vitro diag-
nostics under one or more of the following patens of Applera Corporation: U.S. Patent Nos. 5,656,493,
5,038,852, 5,333,675, 5,475,610 (claims 1-159 and 164-166), 6,703,236 (claims 7-10) and
7,238,517 or corresponding claims in their non-U.S. counterparts. No right is conveyed expressly, by
implication or by estoppels under any other patent of Applera, including but not limited to U.S. Patent
No. 6,814,934 and its non-U.S. counterparts, which describe and claim thermal cyclers capable or
real-time detection.

Instruction manual Primus 25 advanced®
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3SYSTEM OVERVIEW
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1 Heated lid With automatic height adjustment
2 Universal block for 25 x 0.2 ml tubes or 13 x 0.5 ml tubes with flat caps
3 Display Displays menus and programs
4 Cursor key pad Moves the cursor on the display
5 Alphanumeric key pad For entering parameters
6 Alpha key Switches between numbers and letters
7 Shift lock key Switches between lower and upper case
8 Delete key Deletes parameters or functions
9 Insert key Inserts parameters or functions
10 Mode key Selects edit and display options
11 Stop / Esc key (red) Exits a menu option to return to a higher menu level
12 Run / Enter key (green) Selects menus and program options

Instruction manual Primus 25 advanced®
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4INSTALLATION
Before using the Primus 25 advanced® for the first time, check the voltage range and be certain it cor-
responds to your power supply.
Place your Primus 25 advanced® in an adequate position in the lab. The unit should always be sur-
rounded by at least 25 cm of free space to ensure proper cooling. Place several units beside each other,
not behind each other.
Please avoid running the Primus cycler without having tubes placed inside the block.
5MAIN MENU
The software for the Primus 25 advanced® is designed for simple and intuitive use. Generally, the ar-
row keys are used to move the cursor on the display screen. Text is entered using the alphanumeric key
pad. The position of a letter on the key tells how many times the key must be pressed (in rapid sequence)
to obtain that letter. Use the Run/Enter key to access the various function menus, and the Stop/Esc key
to exit the function menus and return to the main menu.
When switching on the instrument, the screen first displays an initialization routine, then the main menu
appears from which all other functions of the Primus 25 advanced® are accessible.
5.1 RUN
To start the last run program select RUN. The program starts immediately.
Attention: The modifications in the program after the last run will be ignored. In this case, select the pro-
gram with SELECT + RUN PROGRAM and press enter to start.
Programs can be interrupted at any time during a run with the Stop/Esc key. Follow the instructions on
the display.
5.2 SELECT + RUN PROGRAM
To run a previously stored program select SELECT + RUN PROGRAM. A list of all stored programs (up
to 90) is displayed. Select the desired program with the arrow keys, or enter the program number di-
rectly. Press enter to start. Before each run, a user name may be entered via the alphanumeric key pad.
At this point you can decide whether a GLP report shall be printed via the parallel printer interface (GLP
ONLINE PRINTS; GLP = Good Laboratory Practice). Switch between YES und NO with the () cursor
keys. A predicted program run time will be displayed before the start.
Programs can be interrupted at any time during a run with the Stop/Esc key. Follow the instructions on
the display.

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5.3 RUN INCUBATION
The Primus 25 advanced® may be used as incubator with the INCUBATION function. Choose the de-
sired temperature (INCUB) and switch the lid heating on or off (LIDHT). The current temperature, the
status of the lid heating and the total time are displayed. Use the Stop/Esc key to exit the INCUBATION
function.
5.4 MODE/EDIT/UTILS
With this menu option you can edit and review all programming and operating functions.
5.4.1 PROGRAM
With the submenu PROGRAM programs may be created and changed..
5.4.2 VIEW
With the VIEW option, an overview of the stored programs may be viewed without risking an accidental
alteration of the data. The GLP report of the last program run can be viewed on the display. Information
about setup, system, and block are available here.
5.4.3 PRINT
With the PRINT menu programs, program lists, GLP reports and equipment information may be printed
via the parallel printer interface.
5.4.4 SETUP
The basic setup of the Primus 25 advanced® is entered via SETUP. To enter the time and date use
CLOCK. If several Primus thermal cyclers are connected via the lab bus (RS485), enter the machine's
address here.

Instruction manual Primus 25 advanced®
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6PROGRAMMING
Thanks to the convenient user interface of the Primus 25 advanced®, it is easy to create PCR programs
of various degrees of complexity.
In the main menu, move the cursor to MODE/EDIT/ UTILS and press Enter twice, or go to the PRO-
GRAM menu with the Mode key.
The following options are available:
NEW To create a completely new program.
EDIT To change an existing program.
COPY To copy a previously stored program (e.g. to modify a program).
DELETE To delete one or all programs.
6.1 Program steps
The following program steps are available for programming the Primus 25 advanced®:
TEMP a) Temperature
b) Temperature change within a program loop. The sign states if the temperature is
increased or decreased.
c) Period of time for which the preset temperature is to be held. Using the „minus“
key "FOREVER" can be chosen
d) Time change within a program loop. The sign indicates whether the time is
increased or decreased.
RAMP The ramp always starts at the temperature defined last and runs to the set temperature.
PAUSE The process is paused for the stated time at the preset temperature. The pause may be inter-
rupted at any time by pressing a key. The thermal cycler emits an audible signal when the
pause is reached. After pressing the key Enter, the program continues with the next step.
LOOP[Start of a program loop which is closed by a subsequent Loop] step. Input the number of
cycles. No “loop within a loop” (nested loops) are possible!
LOOP]End of a program loop.
LIDHT The heated lid may be switched on or off. The stated temperatures are kept constant within
the program after the Lidheat step. Temperatures from 70 °C to 120 °C are valid.
STORE Cooling to 8°C, the time is programmable.
LIDOP The program is stopped and the user is asked to open the lid.
LIDCL The program is stopped and the user is asked to close the lid.
END The END step cannot be programmed or deleted, but is always found at the end of a pro-
gram. When reaching this step, the instrument is actively run to ambient temperature and the
operator is called to the instrument .

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6.2 Sample program
Select MODE, then PROGRAM and create a new program with NEW. Enter a program name and con-
firm with Enter to access the programming functions.
01 LIDHT ON 120 °C Heated lid on, temperature 120 °C
Press Enter to insert a new program step. Move from each parameter to the next with the cursor keys
() or with Enter.
02 TEMP 94.0 °C +0.0 °C Denaturation at 94 °C
0:02:00 +0:00 For 2 minutes
Enter another program step with Enter. Choose one of the steps listed above by using the () cursor
keys, e.g., LOOP[.
03 LOOP[ 30x Open a program loop - 30 cycles
Continue by entering a temperature profile.
04 TEMP 94.0 °C +0.0 °C Denaturation at 94°C
0:00:30 +0:00 For 30 seconds
(Press 0 to enter the time in seconds)
05 TEMP 50.0 °C -0.5 °C Annealing at 50°C with stepwise
0:00:30 +0:00 temperature decrement by 0.5 °C (Touch-
down)
06 TEMP 72.0 °C +0.0 °C Elongation with stepwise time increment
0:00:30 +0:05 by 5 seconds
End the cycling step with LOOP]and with the next step switch the heated lid off.
07 LOOP] End the program loop
08 LIDHT OFF Lid heat off
Ramps may be inserted into any program. The ramp starts from the last programmed temperature (here
72 °C) and decreases temperature to the set temperature (here 8 °C) at a constant rate.
09 RAMP 4.0 °C Target temperature 8 °C with
1.0 °C/s a speed of 1 °C per second
10 STORE 8.0°C +0.0°C Final temperature 8 °C
Forever +0.00 (Minus key!)
11 End End of program

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With the cursor keys () the HEADER of the program is accessible. Here, a comment (CMNT), author
(AUTH), and user (USER) may be input. Also, a denaturation step in case of power failure (PWRFAIL-
DENAT) may be programmed.
Exit the program editor with Stop/Esc. Edited or newly created PCR programs are tested in a simulation
run before being saved and before running. You can only exit the editing mode if the program makes
sense. If not, the editor will indicate possible error sources. A calculated program run time can be dis-
played (CALCTIM), and you can save your newly created PCR program with Enter.
7TECHNICAL SUPPORT AND ORDERING INFORMATION
For technical questions and more detailed information on PEQLAB’s products please visit
www.peqlab.com to find the respective contact person.
This manual is supplied with the following products:
Product Cat. No.
Primus 25 advanced®Personal cycler with universal block
for 25 x 0.2 ml tubes of 13 x 0.5 ml tubes with flat caps
95-4002
110 V version for USA 95-4002-US
As reaction vessels you can use 0.2 ml tubes or 0,5 ml tubes with flat caps. For optimum results, only
use thin-walled tubes with a slim cone.
Item Description Cat. No.
PCR Tubes 0.2 ml natural, 1 bag of 1000 82-0337-A
(domed cap) natural, 5 bags of 1000 82-0337-B
assorted colours, 5 bags of 200 82-0491-A
assorted colours, 25 bags of 200 82-0491-B
PCR Tubes 0.2 ml natural, 1 bag of 1000 82-0620-A
(flat cap) natural, 5 bags of 1000 82-0620-B
assorted colours, 5 bags of 200 82-0622-A
assorted colours, 25 bags of 200 82-0622-B
PCR Tubes 0.5 ml natural, 1 bag of 1000 82-0350-A
(flat cap) natural, 5 bags of 1000 82-0350-B
assorted colours, 5 bags of 200 82-0533-A
assorted colours, 25 bags of 200 82-0533-B