Waters ACQUITY UPC2 Parts list manual

ACQUITY UPC2
System Guide
715004521
Revision C
Copyright © Waters Corporation 2016
All rights reserved

November 16, 2016, 715004521 Rev. C
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General Information
Copyright notice
© 2016 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN
IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE
REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.
The information in this document is subject to change without notice and should not be construed
as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any
errors that may appear in this document. This document is believed to be complete and accurate
at the time of publication. In no event shall Waters Corporation be liable for incidental or
consequential damages in connection with, or arising from, its use. For the most recent revision of
this document, consult the Waters Web site (waters.com).
Trademarks
Waters, “THE SCIENCE OF WHAT’S POSSIBLE.”, ACQUITY, ACQUITY UPC2, ACQUITY UPLC,
UPLC, Connections INSIGHT, Waters Quality Parts, Empower, MassLynx, Viridis and UPC2are
registered trademarks of Waters Corporation, and eCord is a trademark of Waters Corporation.
PEEK is a registered trademark of Victrex Corporation.
PEEKsil s a trademark of SGE International Pty.Ltd.
Nitronic is a registreed trademark of AK Steel Corporation.
Teflon is a registered trademark of E. I. du Pont de Nemours and Company.
Vespel is a registered trademark of E. I. du Pont de Nemours and Company.
Other registered trademarks or trademarks are the sole property of their owners.
Customer comments
Waters’ Technical Communications organization invites you to report any errors that you
encounter in this document or to suggest ideas for otherwise improving it. Help us better
understand what you expect from our documentation so that we can continuously improve its
accuracy and usability.
We seriously consider every customer comment we receive. You can reach us at
[email protected].

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Contacting Waters
Contact Waters with enhancement requests or technical questions regarding the use,
transportation, removal, or disposal of any Waters product. You can reach us via the Internet,
telephone, or conventional mail.
Safety considerations
Some reagents and samples used with Waters instruments and devices can pose chemical,
biological, or radiological hazards (or any combination thereof). You must know the potentially
hazardous effects of all substances you work with. Always follow Good Laboratory Practice, and
consult your organization’s standard operating procedures as well as your local requirements for
safety.
Safety hazard symbol notice
Documentation needs to be consulted in all cases where the symbol is used to find out the
nature of the potential hazard and any actions which have to be taken.
Considerations specific to the UPC2system
Power cord replacement hazard
Waters contact information
Contacting medium Information
Internet The Waters Web site includes contact information for Waters
locations worldwide. Visit www.waters.com.
Telephone and fax From the USA or Canada, phone [phone], or fax
508-872-1990.
For other locations worldwide, phone and fax numbers appear in
the Waters Web site.
Conventional mail Waters Corporation
Global Support Services
34 Maple Street
Milford, MA 01757
USA
Warning: To avoid electric shock, use the SVT-type power cord in the United States and
HAR-type (or better) cord in Europe. The main power cord must be replaced only with one
of adequate rating. For information regarding what cord to use in other countries, contact
your local Waters distributor.

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Hand crush hazard
High voltage hazard
Carbon dioxide hazards to humans
Carbon dioxide exhibits three primary hazards for humans:
• Toxicity at levels above 10,000 ppm (PEL 5000 ppm).
• Frostbite from uncontrolled release of pressurized CO2to atmosphere or contact
with accumulated dry ice at a leak site.
• Asphyxiation caused by the displacement of oxygen.
Before proceeding with any monitoring safety configuration, consult with your
environmental health and safety manager regarding applicable local, federal, and
international safety regulations and requirements.
Bottle placement prohibition
FCC radiation emissions notice
Changes or modifications not expressly approved by the party responsible for compliance, could
void the users authority to operate the equipment. This device complies with Part 15 of the FCC
Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful
Warning: To avoid crushing your fingers beneath or between modules, use extreme care
when installing a module in the system stack.
Warning: To avoid electric shock, do not remove protective panels. The components they
cover are not user-serviceable.
Warning: To prevent injury or death, install a CO2Ambient Air Sensor/Alarm unit
to ensure compliance with OSHA PEL for CO2 in locations where CO2is used or
stored, instead of or in addition to, an oxygen monitor. Monitors must be
capable of detecting CO2levels at a minimum of 5000 ppm or as required by
your local regulating agency.
Warning: To avoid injury from electrical shock or fire, and damage to the equipment, do not place
vessels containing liquid atop the workstation or ancillary equipment or otherwise expose those
units to dripping or splashing liquids.
Prohibited: Do not place vessels containing liquid—such as solvent bottles—atop the
workstation or ancillary equipment or otherwise expose those units to dripping or splashing
liquids.

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interference, and (2) this device must accept any interference received, including interference that
may cause undesired operation.
Electrical power safety notice
Do not position the instrument so that it is difficult to disconnect the power cord.
Equipment misuse notice
If equipment is used in a manner not specified by its manufacturer, protections against personal
injury inherent in the equipment’s design can be rendered ineffective.
Safety advisories
Consult Appendix A for a comprehensive list of warning advisories and notices.
Operating this system
When operating this system, follow standard quality-control (QC) procedures and the guidelines
presented in this section.
Applicable symbols
Symbol Definition
Manufacturer
Date of manufacture
Authorized representative of the European Community
Confirms that a manufactured product complies with all applicable
European Community directives
or
Australia EMC compliant
Confirms that a manufactured product complies with all applicable
United States and Canadian safety requirements
Consult instructions for use

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Audience and purpose
This guide provides the user with information for setting up, operating, and maintaining the
ACQUITY UPC2system. This guide is intended for personnel who operate and maintain the
ACQUITY UPC2system. This guide should be used in conjunction with instruction and training
provided by Waters personnel.
Intended use of the UPC2system
Waters designed the ACQUITY UPC2system to perform normal phase separations in
pharmaceutical development, discovery, and QA and QC environments, as well as, within the
chemical materials, environmental, and food safety environments. The ACQUITY UPC2system is
not intended for use in diagnostic applications.
Quality control
Routinely run three QC samples that represent subnormal, normal, and above-normal levels of a
compound. If sample trays are the same or very similar, vary the location of the QC samples in the
trays. Ensure that QC sample results fall within an acceptable range, and evaluate precision from
day to day and run to run. Data collected when QC samples are out of range might not be valid. Do
not report these data until you are certain that the instrument performs satisfactorily.
EMC considerations
Canada spectrum management emissions notice
This class A digital product apparatus complies with Canadian ICES-001.
Cet appareil numérique de la classe A est conforme à la norme NMB-001.
Alternating current
Electrical and electronic equipment with this symbol may contain
hazardous substances and should not be disposed of as general
waste.
For compliance with the Waste Electrical and Electronic Equipment
Directive (WEEE) 2012/19/EU, contact Waters Corporation for the
correct disposal and recycling instructions.
Serial number
Part number catalog number
Symbol Definition
5()

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ISM Classification: ISM Group 1 Class B
This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and
Medical (ISM) instruments requirements.
Group 1 products apply to intentionally generated and/or used conductively coupled
radio-frequency energy that is necessary for the internal functioning of the equipment.
Class B products are suitable for use in both commercial and residential locations and can be
directly connected to a low voltage, power-supply network.
EC authorized representative
Address Waters Corporation
Stamford Avenue
Altrincham Road
Wilmslow SK9 4AX UK
Telephone: [phone]
Fax: [phone]
Contact: Quality manager

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General Information ............................................................................................... iii
Copyright notice ......................................................................................................................... iii
Trademarks ................................................................................................................................ iii
Customer comments .................................................................................................................. iii
Contacting Waters ..................................................................................................................... iv
Safety considerations ................................................................................................................ iv
Safety hazard symbol notice........................................................................................ iv
Considerations specific to the UPC2 system............................................................... iv
FCC radiation emissions notice .................................................................................... v
Electrical power safety notice ...................................................................................... vi
Equipment misuse notice............................................................................................. vi
Safety advisories ......................................................................................................... vi
Operating this system ................................................................................................................ vi
Applicable symbols ...................................................................................................... vi
Audience and purpose................................................................................................ vii
Intended use of the UPC2 system .............................................................................. vii
Quality control............................................................................................................. vii
EMC considerations .................................................................................................................. vii
Canada spectrum management emissions notice...................................................... vii
ISM Classification: ISM Group 1 Class B ................................................................... viii
EC authorized representative ................................................................................................... viii
1 ACQUITY UPC2system ........................................................................................ 13
1.1 Using ACQUITY UPC2for normal-phase chromatography ....................................................... 13
1.1.1 Comparing supercritical fluid properties to those of other solvents ............................... 14
1.2 System configurations ............................................................................................................... 14
1.2.1 ACQUITY UPC2 core system stack configurations and supported modules ................ 15
1.2.2 Major components of the ACQUITY UPC2system........................................................ 16
1.2.3 Waste collection containers ........................................................................................... 18
2 Preparing for operation ........................................................................................ 21
2.1 Start the system hardware ......................................................................................................... 21
2.1.1 Before you power-on the system modules .................................................................... 21
2.1.2 Powering-on the system modules.................................................................................. 22
Table of Contents

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2.2 Starting the console software .................................................................................................... 23
2.2.1 Starting the console ....................................................................................................... 24
2.3 Priming and equilibrating the system ........................................................................................ 25
2.3.1 Priming the system ........................................................................................................ 25
2.3.2 Equilibrating the system................................................................................................. 26
2.4 Observing the sample-injection sequence ................................................................................ 26
2.5 Performing column screening .................................................................................................... 32
2.6 Shutting down the ACQUITY UPC2system .............................................................................. 33
3 Method parameters ............................................................................................... 35
3.1 Creating methods in Empower software .................................................................................... 35
3.2 Creating instrument methods for the UPC2modules ................................................................ 35
3.2.1 ccBSM instrument method settings ............................................................................... 35
3.2.2 SM-FL instrument method settings................................................................................ 36
3.2.3 Instrument method settings for the column manager .................................................... 36
3.2.4 Instrument method settings for the ccManager ............................................................. 36
3.2.5 ACQUITY UPC2PDA instrument method settings ........................................................ 38
3.3 Creating methods in MassLynx software ................................................................................... 38
4 External connections ............................................................................................ 39
4.1 External connections ................................................................................................................. 40
4.2 Connecting Ethernet cables ...................................................................................................... 40
4.2.1 Connecting external signal cables ................................................................................. 41
4.2.2 Connecting the UPC2PDA detector signal cables ........................................................ 44
4.3 Connecting the system to an electricity source ......................................................................... 44
5 Splitter configurations .......................................................................................... 47
5.1 Splitter components ................................................................................................................... 47
5.1.1 Components of the core 1, 2, and 3 splitters ................................................................. 47
5.1.2 Components of the core 4 splitter.................................................................................. 49
5.1.3 Isocratic solvent manager splitter .................................................................................. 50

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5.2 Splitter bracket mount locations ................................................................................................ 52
5.3 Core 1 configuration: system diagrams and tubing connections ............................................... 54
5.4 Core 2 configuration: system diagrams and tubing connections ............................................... 62
5.5 Core 3 configuration: system diagrams and tubing connections ............................................... 65
5.6 Core 4 configuration: system diagrams and tubing connections ............................................... 70
6 Maintenance .......................................................................................................... 77
6.1 Maintaining the ACQUITY UPC2system modules .................................................................... 77
6.2 Maintaining the ABPR ............................................................................................................... 77
6.2.1 Running an ABPR health test when the system includes a CM-30S............................. 77
6.3 Spare parts ................................................................................................................................ 79
6.4 Troubleshooting with Connections INSIGHT ............................................................................. 79
6.5 Safety and handling ................................................................................................................... 80
A Safety Advisories ................................................................................................. 83
A.1 Warning symbols ....................................................................................................................... 83
A.1.1 Specific warnings........................................................................................................... 84
A.2 Notices ...................................................................................................................................... 85
A.3 Bottles Prohibited symbol ......................................................................................................... 85
A.4 Required protection ................................................................................................................... 85
A.5 Warnings that apply to all Waters instruments and devices ...................................................... 86
A.6 Warnings that address the replacing of fuses ........................................................................... 89
A.7 Electrical and handling symbols ................................................................................................ 90
A.7.1 Electrical symbols.......................................................................................................... 90
A.7.2 Handling symbols .......................................................................................................... 91

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B Specifications ....................................................................................................... 93
B.1 System specifications ............................................................................................................... 93
B.2 Solvent specifications ............................................................................................................... 95
B.3 Wetted materials ....................................................................................................................... 95
B.4 ccBSM specifications ................................................................................................................ 96
B.5 ccManager specifications .......................................................................................................... 98
B.6 ACQUITY column manager with active preheating and auxiliary column
manager specifications ............................................................................................................ 99
B.7 ACQUITY 30-cm single-zone column manager specifications ............................................... 100
B.8 ACQUITY UPC2Photodiode array detector specifications ..................................................... 101
B.9 Sample manager - fixed loop specifications ........................................................................... 101
B.10 Physical specifications .......................................................................................................... 103
C Configuring the core 4 system variant ............................................................. 105
C.1 Physical arrangement ............................................................................................................. 105
C.2 Column configuration .............................................................................................................. 106
C.3 Configuring software for dual-column managers .................................................................... 106

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1ACQUITY UPC2system
The ACQUITY®UPC2® (Ultra Performance Convergence Chromatography) system uses
liquid carbon dioxide (CO2) as the primary mobile phase for normal-phase, chiral, and
achiral separations.
You control the system using the driver pack software that you access through one of these
Waters’ data systems: Empower®software or MassLynx®software.
1.1 Using ACQUITY UPC2for normal-phase
chromatography
ACQUITY UPC2chromatography is a normal-phase chromatographic technique that offers these
advantages over traditional HPLC normal-phase chromatography:
• Uses liquid CO2, a chemically pure, stable, and nonpolar solvent, as the primary mobile
phase.
• Combines liquid CO2with one or more organic solvents, typically alcohols such as methanol
and isopropanol, to increase the solvent strength for separating polar compounds.
• Minimizes health, safety, and environmental concerns associated with chemical solvents.
• Runs at higher flow rates (3 to 5 times higher than HPLC for the columns of the same
dimension) because of its high diffusivity.
• Produces lower pressure drops across the column and faster separations, because of the
mobile phases’s low viscosity.
Figure 1–1: Pressure-temperature phase diagram for CO2
75 bar
Pressure
Temperature

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1.1.1 Comparing supercritical fluid properties to those of other
solvents
Supercritical CO2 fluid has a lower viscosity, higher diffusion rate, and no surface tension when
compared to liquid CO2. Supercritical CO2 physical properties are between those of liquids and
gases. Diffusivities of solutes in supercritical CO2are by as much as a factor of 10 higher than their
diffusivities in liquid solvents. Because supercritical fluid properties are pressure-dependent near
the critical point, they are highly tunable solvents.
Recommendation: Use only food-grade, or better, house-supplied CO2or non-siphon CO2at a
flow pressure of 6,205 to 7,584 kPa (62 to 76 bar, 900 to 1100 psi).
1.2 System configurations
The following illustrations show the four core configurations that comprise the ACQUITY UPC2
system. A system configuration can differ according to the number and type of modules it contains.
For example, if a system includes a mass spectrometer, the system modules can be arranged as
two or three separate stacks.
Table 1–1: Solvent viscosity comparison
Solvent Viscosity range
Supercritical CO2 fluid 20-100 µPa.s (0.02-0.1 cP)
Liquids 500-1000 µPa.s (0.5-1.0 cP)
Gases 10 µPa.s (0.01 cP)

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1.2.1 ACQUITY UPC2core system stack configurations and
supported modules
Figure 1–2: Core 1 configuration (2-column system)
Figure 1–3: Core 2 configuration (4-column system)
73
PDA
CM-A
ccManager
SM-FL
ccBSM
Side mount
solvent tray
TP03523
ccManager
SM-FL
ccBSM
Solvent
tray
PDA
CM-AUX
CM-A

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Figure 1–4: Core 3 configuration (6-column system)
Figure 1–5: Core 4 configuration (up to 8-column system)
See also: Appendix C for information about configuring a system with two CM-30S modules.
1.2.2 Major components of the ACQUITY UPC2system
The following table describes system modules and their functions:
TP03529
Solvent
tray
SM-FL
ccBSM
PDA
CM-AUX
CM-A
CM-AUX
ccManager
CM-30S
Solvent
tray
ccManager
SM-FL
PDA
ccBSM

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Table 1–2: Major system components
Module Description
ACQUITY UPC2
Convergence manager
(ccManager)
Regulates and monitors the incoming flow and pressure of liquid CO2
in the system through these mechanisms:
• Electronically controlled CO2inlet shutoff valve
• Overpressure protection
• Auxiliary valve for venting the injection loop
• Automated back pressure regulator (ABPR)
ACQUITY UPC2
Sample manager - fixed
loop
(SM-FL)
Controls sample injections using a fixed-loop injector. It also monitors
and regulates the depressurization of CO2in the flow path during an
injection sequence.
Column manager (CM-A)
and auxiliary column
manager (CM-AUX)
Regulates the column temperature to between 4 and 90 ºC. The
temperature of each column trough can be independently set in
0.1 ºC increments. A supplementary pre-heating device (active
preheater) can also be installed, to heat the incoming solvent before
it enters the column. The CM-A can accommodate two 100- or
150-mm columns with inside diameters of up to 4.6 mm.
The system can include as many as two CM-AUX modules. The
CM-AUX modules can each accommodate as many as two columns
ranging in size from 50- to 150-mm lengths with internal dimensions
from 2.1 to 4.6 mm.
Additionally, the CM-Aux module includes two independently heated
or cooled column troughs, for inlet configuration on the left-hand or
right-hand side. Each column position includes an active pre-heater
for both configurations, to ensure specified thermodynamic
conditions of the column over the range of allowable ambient
conditions.
ACQUITY 30-cm
single-zone column
manager
(CM-30S)
One CM-30S provides support for as many as eight columns in a
single temperature zone. Under Empower software control, two
CM-30S provide support for as many as 15 columns in two heated
temperature zones (5 ºC above ambient to 90 ºC). Columns range in
size from 2.1 to 8.0-mm (internal diameter) and 30 mm to 300 mm
long.
ACQUITY UPC2
Binary solvent manager
(ccBSM)
Under Empower, or MassLynx software control, the pump’s A side
actively chills the CO2fluid pumped into the system while the pump’s
B side can pump any one of four co-solvents. The pump operates at
flow rates up to 4 mL/min and pressures up to 41,369 kPa (413 bar,
6000 psi).
ACQUITY UPC2
Photodiode array (PDA)
detector
Incorporates a 10-mm stainless steel flow cell that can withstand
pressures as high as 41,369 kPa (413 bar, 6000 psi). Updated lens
material improves sensitivity and performance. Reduced internal
volume in the flow cell minimizes dispersion within the cell.
Side-mounted solvent
tray Provides easy access to the solvent bottles in an ACQUITY UPC2
system.
Top-mounted solvent tray The optional top-mounted solvent tray is available for configurations
of four or fewer modules in a stack.

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1.2.3 Waste collection containers
Use only waste collection containers required by your local regulating agency with the ACQUITY
UPC2system.
Warning: To avoid serious injury never use a glass waste container with an ACQUITY
UPC2system because it can burst in the event of over-pressure conditions.
Notice: To avoid fluid backup, ensure proper drainage of waste:
• Place the waste container below the system stack.
• Ensure that the waste tube does not crimp or bend. A crimp or bend can impede flow to
the waste container.
• Ensure that the exit of the waste tube is not immersed in waste solvent. If necessary,
shorten the tube so that no portion of it drops below the top of the waste container.

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Figure 1–6: Solvent flow path through a Core 1 system

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