Westlab 665-251 User manual

1
PRODUCT MANUAL
Precision Single
Channel Pipettes
Codes 665-251, 665-252, 665-254, 665-255, 665-256, 665-257, 665-258

2
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
1. Product Description
1.1 Introduction
Westlab’s Precision Single Channel Pipettes are designed to meet the liquid-transfer
requirements of the modern laboratory and to withstand chemical exposure.
With excellent calibration retention and a precise volume adjustment mechanism,
the pipettes ensure accurate volume dispensing. Coupled with the lockable counter
mechanism, the high precision volume adjustment of the pipettes commoditises
accurate and repeatable liquid dispensing.
Tailored to reduce the negative effects from repetitive strain injury, the well-balanced and
sturdy chassis reduces unnecessary weight without compromising the overall quality of
the pipette. Furthermore, the pipettes are manufactured from a heat-resistant material
allowing them to be safely autoclaved multiple times without a loss of quality. With a fully
autoclavable body, the transfer of contaminants during pipetting is reduced.
The pipettes are provided with exchangeable colour inserts for easy visual identication
of pipettes by user-dened or volume-dened categories. Each pipette can be individually
colour coded, using the included tool for drop removing.
The pipettes operate according to the air displacement principle and are produced and
tested according to EN ISO 8655.
1.2 Features
• Fully Autoclavable Pipette Body
• Soft Grip & Light Pipette Chassis
• Exchangeable Colour Inserts for Visual Identication
• Serial Number & Declaration of Conformity Supplied
• Counter-Mechanism Prevents Accidental Turning
• Compatible With All Major Pipette Tip Brands
• Manufactured In Germany
TABLE OF CONTENT
1. Product Description
1.1 Introduction
1.2 Features
1.3 Accessories
2. Technical Specications
3. Safety Precautions
4. Maintenance & Cleaning
5. Installation
6. Operation
7. Troubleshooting
8. Product Disposal
PRODUCT CODE
665-251
665-252
665-254
665-255
665-256
665-257
665-258
PLEASE READ THESE INSTRUCTIONS BEFORE USE.
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y

3
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
1.3 Accessories
• Single channel pipette
• Silicone grease
• Calibration/assembling tool
• Tool to remove colour coding drop
• Bag of drops for colour coding
• Bag of sample tips
• Instruction manual
• Calibration report
2. Technical Specications
Code
Variable
Volume Increments Test Volume Inaccuracy Imprecision
665-251 0.5 - 10µl 0.1µl 1µl
5µl
10µl
2.5%
2.0%
1.0%
1.50%
1.00%
0.80%
665-252 2 - 20µl 0.1µl 2µl
10µl
20µl
3.0%
1.5%
0.9%
2.00%
1.00%
0.40%
665-254 10 - 100µl 0.5µl 10µl
50µl
100µl
3.0%
1.0%
0.8%
1.50%
0.50%
0.15%
665-255 20 - 200µl 1µl 20µl
100µl
200µl
2.0%
0.7%
0.6%
0.80%
0.30%
0.15%
665-256 100 -
1000µl
5µl 100µl
500µl
1000µl
2.0%
1.0%
0.6%
0.70%
0.40%
0.20%
665-257 0.5 - 5ml 50µl 500µl
2500µl
5000µl
2.0%
0.6%
0.5%
0.60%
0.30%
0.15%
665-258 1 - 10ml --3.0% 0.60%

4
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
3. Safety Precautions
Read the following safety precautions prior to using the single channel pipette.
Never use the unit in any manner not specied in this manual.
• Wear personal protective equipment during the operation of the unit.
• Do not use the pipette if it shows signs of electrical of mechanical damage.
If any damage is found, contact your supplier immediately.
• Do not use without appropriate training. Always follow the installation instructions.
The safety of the user cannot be guaranteed if the unit:
• Is operated with accessories that are not supplied with the unit
• Is operated with accessories that are not supplied or recommended by
the manufacturer
• Is operated improperly or contrary to the manufacturer’s specications
4. Maintenance & Cleaning
• Wear protective gloves and safety glasses while cleaning the unit.
• After cleaning, ensure that all the parts are dried.
• If repairs are performed by unauthorised personnel, the product warranty may
become void.
• It is recommended to use normal cleaning agents such as water with mild
detergents, antiseptic agents or 60-70% ethanol.
• Recommended to check the pipette’s performance every three months.
Pay attention to the tip cone.
5. Installation
1. Open the packaging and gently remove the single channel pipette from the box.
2. Unpack every individual component.
3. Assemble the components as instructed in this manual for optimal performance.
ATTENTION: The manual should be kept with the device and all the packaging should be
kept in safe storage for at least two years for warranty purposes.
.

•
5
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Top assembly parts
Number Name
1Push button
2Top closure
3Spring stopper
4Primary spring support
5Primary spring
6Main body
7Counter
Number Name
8Washer
9Shaft
10 Bottom closure snap tting
11 Tip ejector
12 Tip ejector spring
13 Drop
14 Tip ejector pusher support
NOTE: The bottom closure snap
tting and the tip ejector pusher are
different sizes for the single and
multi-channel pipettes. The tip ejector
pusher support is only available in the
multichannel pipette.

6
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Bottom assembly parts
Number Name
1Piston
2Piston spring
3Piston support
4Tip cone tip
5O-ring
6Tip cone
7Ejector collar
Number Name
8Secondary spring [100 - 1000µl]
9Secondary spring support
10 Washer [500 - 5000µl]
11 Washer [500 - 5000µl]
12 Spring [500 - 5000µl]
13 Washer [500 - 5000µl]
14 O-ring holder
NOTE: For the 10,000µl and the 2000
to 10,000µl pipettes, the tip ejection
features is not available. The tip
cone, washer [500 - 5,000µl] and the
O-ring holder are of different sizes for
volume 500 to 5,000µl and 200
to 10,000µl.

•
7
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Calibration/disassembling tool
The calibration/disassembling tool is supplied for maintenance and calibration
of the pipette.
Pipette identication
The pipettes are provided with exchangeable colour inserts for easy visual
identication of pipettes by user-dened or volume-dened categories.
Disassembling
of the tip
ejector or
ejector collar.
Used for
calibration.
Disassembling
of the tip cone.

•
8
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Disassembling and reassembling
1. Hold down the tip ejector.
2. Place the tooth of the opening tool
between the tip ejector and the tip
ejector collar to release the locking
mechanism.
3. Carefully release the tip ejector and
remove the ejector collar.
4. Place the wrench end of the opening
tool over the tip cone and turn
clockwise.
5. After removing the tip cone, wipe the
piston and the tip
cone with ethanol and a lint
free cloth.
6. Before replacing the tip cone, grease
the piston slightly using the supplied
silicon grease.
7. After reassembling, use the pipette
several times to ensure that the
grease is spread evenly.
8. Check the calibration of
the pipette.

•
9
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
6. Operation
Number Name
1Push button Used to aspirate and dispense the liquid.
To set the volume.
2Top closure Used to adjust the calibration with the help of
the calibration tool.
3Drop The drop is colour coded for easy identication.
4Tip ejector The tip ejector moves the ejector collar and
ejects the pipette tip.
5Volume printed Indicates the volume of the pipette.
6Main body
7Ejector collar Used to eject the pipette tip.
8Tip cone Where the pipette tip is tted.
9Volume display Shows the set volume.
10 Body grip Prevents the transfer of body heat to
internal components.

•
10
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Pipetting recommendations
• Aspirate liquid into the pipette only when a tip is attached. Hold the pipette vertically
when aspirating and dip the tip only 2-3mm into the liquid.
• Pre-rinse the tip ve times before aspiration by lling and emptying the tip.
• Always control the push button movements with the thumb for consistency.
Allow the liquid, tip and pipette to equilibrate to the ambient temperature before use.
• When not in use, place the pipette on a bench to avoid transferring body heat
resulting in incorrect dispensing volume
• Use the correct pipette tip designed for the use with the particular pipette.
• Select the correct pipetting technique (e.g. reverse, forward pipetting) depending
on the nature of the liquid.
• Don‘t lay down the pipette with the lled tip, otherwise the liquid can ow into the
pipette and contaminate it
General operation
Volume setting
1. The volume is shown in the handgrip display window.
2. The desired volume is set by turning the push button clockwise or anticlockwise.
ATTENTION! Using excessive force to turn the push button may jam the mechanism
and damage the pipette.