Westlab 665-259 User manual

1
PRODUCT MANUAL
Precision
Multichannel
Pipettes
Codes 665-259, 665-260, 665-261, 665-262, 665-263, 665-264

2
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
1. Product Description
1.1 Introduction
Westlab’s Precision Multichannel Channel Pipettes are designed to meet the liquid-
transfer requirements of the modern laboratory and to withstand chemical exposure.
The metal fabrication of the pipettes increases their liquid transfer precision and
accuracy. Additionally, the 8- and 12-channel variants of the multichannel pipettes offer
pipetting volume exibility.
With excellent calibration retention and a precise volume adjustment mechanism,
the pipettes ensure accurate volume dispensing. Coupled with the lockable counter
mechanism, the high precision volume adjustment of the pipettes commoditises
accurate and repeatable liquid dispensing.
Tailored to reduce the negative effects from repetitive strain injury, the well-balanced and
sturdy chassis reduces unnecessary weight without compromising the overall quality of
the pipette. Furthermore, the pipettes are manufactured from a heat-resistant material
allowing them to be safely autoclaved multiple times without a loss of quality. With a fully
autoclavable body, the transfer of contaminants during pipetting is reduced.
The pipettes are provided with exchangeable colour inserts for easy visual identication
of pipettes by user-dened or volume-dened categories. Each pipette can be individually
colour coded, using the included tool for drop removing.
The pipettes operate according to the air displacement principle and are produced and
tested according to EN ISO 8655.
1.2 Features
• Fully Autoclavable Pipette Body
• Soft Grip & Light Pipette Chassis
• Exchangeable Colour Inserts for Visual Identication
• Serial Number & Declaration of Conformity Supplied
• Accurate Liquid Dispensing Across All Channels
• Counter-Mechanism Prevents Accidental Turning
• Compatible With All Major Pipette Tip Brands
• Manufactured In Germany
TABLE OF CONTENT
1. Product Description
1.1 Introduction
1.2 Features
1.3 Accessories
2. Technical Specications
3. Safety Precautions
4. Maintenance & Cleaning
5. Installation
6. Operation
7. Troubleshooting
8. Product Disposal
PRODUCT CODE
665-259
665-260
665-261
665-262
665-263
665-264
PLEASE READ THESE INSTRUCTIONS BEFORE USE.
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3
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
1.3 Accessories
Package includes:
• Single channel pipette
• Silicone grease
• Calibration/assembling tool
• Tool to remove colour coding drop
• Bag of drops for colour coding
• Bag of sample tips
• Bag of O-rings
• Instruction manual
• Calibration report
2. Technical Specications
Code Channels Variable Volume Inaccuracy Imprecision
665-259 80.5 - 10µl 5.00% / 2.00% 3.60% / 1.00%
665-260 810 - 100µl 2.50% / 1.30% 120% / 0.50%
665-261 830 - 300µl 1.66% / 1.40% 0.60% / 0.30%
665-262 12 0.5 - 10µl 5.00% / 2.00% 3.60% / 1.00%
665-263 12 10 - 100µl 2.50% / 1.30% 120% / 0.50%
665-264 12 30 - 300µl 1.66% / 1.40% 0.60% / 0.30%

4
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
3. Safety Precautions
Read the following safety precautions prior to using the multichannel pipette.
Never use the unit in any manner not specied in this manual.
• Wear personal protective equipment during the operation of the unit.
• Do not use the pipette if it shows signs of electrical of mechanical damage. If any
damage is found, contact your supplier immediately.
• Do not use without appropriate training. Always follow the installation instructions.
The safety of the user cannot be guaranteed if the unit:
• Is operated with accessories that are not supplied with the unit.
• Is operated with accessories that are not supplied or recommended by
the manufacturer.
• Is operated improperly or contrary to the manufacturer’s specications.
4. Maintenance & Cleaning
• Wear protective gloves and safety glasses while cleaning the unit.
• After cleaning, ensure that all the parts are dried.
• If repairs are performed by unauthorised personnel, the product warranty may
become void.
• It is recommended to use normal cleaning agents such as water with mild
detergents, antiseptic agents or 60-70% ethanol.
• Recommended to check the pipette’s performance every three months.
Pay attention to the tip cone.
5. Installation
1. Open the packaging and gently remove the multichannel pipette from the box.
2. Unpack every individual component.
3. Assemble the components as instructed in this manual for optimal performance..
ATTENTION: The manual should be kept with the device and all the packaging should be
kept in safe storage for at least two years for warranty purposes.
.

•
5
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Top assembly parts
Number Name
1Push button
2Top closure
3Spring stopper
4Primary spring support
5Primary spring
6Main body
7Counter
Number Name
8Washer
9Shaft
10 Bottom closure snap tting
11 Tip ejector
12 Tip ejector spring
13 Drop
14 Tip ejector pusher support
NOTE: The bottom closure snap
tting and the tip ejector pusher are
different sizes for the single and
multi-channel pipettes. The tip ejector
pusher support is only available in the
multichannel pipette.

6
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Bottom assembly parts
Number Name
1Piston housing
2Steel piston
3Tip cone housing
4Sealing O-rings
5Tip cone outer O-rings

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7
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Calibration/disassembling tool
The calibration/disassembling tool is supplied for maintenance and calibration
of the pipette.
Pipette identication
The pipettes are provided with exchangeable colour inserts for easy visual
identication of pipettes by user-dened or volume-dened categories.
Disassembling
of the tip
ejector or
ejector collar.
Used for
calibration.
Disassembling
of the tip cone.

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8
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Disassembling and reassembling
1. Hold down the tip ejector.
2. Place the tooth of the opening tool
between the tip ejector and the tip
ejector collar to release the locking
mechanism.
3. Carefully release the tip ejector and
remove the ejector collar.
4. Place the wrench end of the opening
tool over the tip cone and turn
clockwise.
5. After removing the tip cone, wipe the
piston and the tip cone with ethanol
and a lint free cloth.
6. Before replacing the tip cone, grease
the piston slightly using the supplied
silicon grease.
7. After reassembling, use the pipette
several times to ensure that the
grease is spread evenly.
8. Check the calibration of the pipette.

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9
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
6. Operation
Number Name
1Ejector collar Used to eject the pipette tips.
2Tip cone Where the pipette tips are tted.
Pipetting recommendations
• Aspirate liquid into the pipette only when the tips are attached. Hold the pipette
vertically when aspirating and dip the tips only 2-3mm into the liquid.
• Pre-rinse the tips ve times before aspiration by lling and emptying the tips.
• Always control the push button movements with the thumb for consistency.
Allow the liquid, tips and pipette to equilibrate to the ambient temperature before use.
• When not in use, place the pipette on a bench to avoid transferring body heat
resulting in incorrect dispensing volume
• Use the correct pipette tips designed for the use with the particular pipette.
• Select the correct pipetting technique (e.g. reverse, forward pipetting) depending on
the nature of the liquid.
• Don‘t lay down the pipette with the lled tip, otherwise the liquid can ow into the
pipette and contaminate it.

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10
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
General operation
Volume setting
1. The volume is shown in the handgrip display window.
2. The desired volume is set by turning the push button clockwise or anticlockwise.
ATTENTION! Using excessive force to turn the push button may jam the mechanism
and damage the pipette.
Loading and ejecting tips
1. Ensure that the tip cone is clean.
2. Press the tip rmly onto the tip cone to ensure an airtight seal.
3. The pipettes are equipped with a tip ejector to help eliminate the tip and to avoid
safety hazards associated with contamination. The tip ejector needs to be pressed
downwards to ensure proper tip ejection.

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11
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Forward pipetting
1. Press the push button to the rst stop.
2. Dip the attached tip into the liquid vertically by 2 to 3mm.
Smoothly release the push button.
3. Withdraw the tip slowly from the liquid - touching against the wall of the vessel
to remove remaining liquid. Place the tip on the wall of the vessel on an angle.
Dispense the liquid into the receiving vessel by gently pressing the push button
to the rst stop.
4. After a short delay, press the push button to the second stop.
This will empty the tip completely and ensure accurate pipetting.
5. Release the push button to the ready position..
Reverse pipetting
Reverse pipetting is recommended for viscous solutions with a tendency to foam or
dispense very small volumes.
1. Press the push button to the second stop.
2. Dip the attached tip into the liquid vertically by 2 to 3mm. Smoothly release the
push button. This will ll the tip with an additional volume larger than the set
volume.
3. Withdraw the tip slowly from liquid - touching against the wall of the vessel to
remove remaining liquid. Dispense the liquid into the receiving vessel by gently
pressing the push button to the rst stop. This volume is equal to the set volume.
4. Hold the push button in this position. The liquid that remains in the tip should not
be dispensed. The remained liquid can be discarded with the tip or delivered
back into the vessel with the original solution.
5. Release the push button to the ready position.

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1. Place the calibration tool into
the holes of the calibration
adjustment lock (under the
push button).
2. Turn the adjustment lock
anticlockwise to decrease and
clockwise to increase the volume.
3. Repeat checking calibration until
the pipetting results are correct.
12
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
Autoclaving
The pipettes are fully autoclavable by steam at 121°C, 1.05 bar for 15 min.
Autoclave the complete pipette without any disassembling.
After autoclaving under the mentioned conditions, allow the pipette to cool and dry
for 6 hours before use. It is not required to re-calibrate the pipette after autoclaving.
If the pipette is autoclaved frequently, the piston and springs should be greased with
the lubricant supplied with each pipette to maintain smooth movement.
Calibration
Performance testing should take place in a draught-free, stable environment with a
relative humidity above 50% and constant (±0,5 °C) temperature between 15°C and 30°C.
The weighing vessel, pipette, tips, and distilled water need to have been in the room for a
sucient time, at least 2 hours, to reach equilibrium with the room conditions.
Use an analytical balance with a readability of 0.01 mgs. 10 measurements for each test
volume shall be carried out. For variable volume pipettes, at least three volumes shall be
tested (nominal volume, 50% of nominal volume, and 10% of nominal volume).
1. Select the test volume. Do not change the setting during the test cycle of
10 measurements.
2. Fit the selected tips to the pipette cones.
3. Fill the tips with distilled water and condition the pipette before testing by aspirating
and dispensing the distilled water in the tips ve times. Afterwards discard the tips.
4. Attach new tips on the pipette cones and pre-wet the tips once.
5. Aspirate the distilled water and pipette it into the weighing vessel
(use forward pipetting technique).
6. Weigh the pipetted quantity with an analytical balance and record the weight.
Repeat the test cycle until 10 measurements have been recorded.
7. Convert the recorded masses to volumes, calculate the mean volume,
systematic error and random error as described in EN ISO 8655-6:2002, part 8.
8. Compare the systematic error (inaccuracy) and the random error (imprecision)
with the values in the specication table.
The calibration of the pipette must be set even if only one of the results falls outside
the permitted range.
For checking the calibration of a multichannel pipette, each channel should be
considered as an individual pipette.
Recalibration

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13
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
7. Troubleshooting
Please contact our technical support team on 1800 358 101.
Error Issue Solution
Droplets left
inside the
tip
Unsuitable tip, non-
uniform wetting of the
plastic
Use a new tip.
Leakage
or pipetted
volume is
too small
Tip cone is scratched or
damaged Change the tip cone.
Organic solvent as liquid
Aspirate and discard the organic
solvent several times before actually
pipetting with the same tip.
Tip incorrectly attached Attach the tip rmly.
Unsuitable tip Use a new tip.
Foreign particles between
the tip and the tip cone Clean the tip cone.
Insucient amount of
grease on the piston Clean and grease the piston.
Inaccuracies
Incorrect operation Follow instructions carefully,
Calibration altered Recalibrate according to the
instructions.
Unsuitable for the
particular liquid pipetting
technique
Use the correct pipetting technique.
Pipette damaged Contact our technical support team
on 1800 358 101.
Push button
jammed
or moves
erratically
Piston contaminated
Clean and grease the piston.
Penetration of solvent
vapours
Tip ejector
is jammed
or moves
erratically
Tip cone is contaminated
from the outside
Remove the ejector collar and clean
the outer surface of the tip cone
with ethanol.
Volume
setting
isn’t ‘click-
stopping’
properly
Click stop mechanism
is damaged
Contact our technical support team
on 1800 358 101.
Push button
doesn’t turn
for volume
setting
Use of excessive force
beyond the range of
the pipette
Contact our technical support team
on 1800 358 101.

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Phone
1800 358 101
Speak to one of our friendly,
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24 hours, 7 days a week
convenience.
14
PRODUCT MANUAL
1800 358 101
[e-mail]
www.westlab.com.au
[phone]
[e-mail]
www.westlab.co.nz
646 568 5391
[e-mail]
www.westlab.com
1 877 822 1455
[e-mail]
www.westlab.com
Australia New Zealand United StatesCanada
8. Product Recycling
In the case that the product is to be disposed of, the relevant legal regulations are to
be observed.

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PRODUCT MANUAL
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