Ysi We know proobod User manual

USER MANUAL

YSI Environmental

Professional Series Overlay Art

ProOBOD

linserpelle creative

513-831-1650

8.09

CONTENTS

Warranty ..............................................................................................................i

Introduction ....................................................................................................... 1

Getting Started ................................................................................................... 2

Unpacking the OBOD Probe............................................................. 2

POBOD Operation........................................................................................ 4

Connecting Probe/Cable Assembly to Instrument ......................... 4

Configuring the PODO Instrument............................................. 4

Powering the Stirrer............................................................................ 5

Calibration - Dissolved Oxygen ........................................................ 5

Temperature......................................................................................... 7

Taking Measurements......................................................................... 7

Maintenance and Storage .................................................................................. 8

Updating Instrument and Probe Firmware...................................... 8

Sensor Maintenance - Dissolved Oxygen......................................... 8

Sensor Maintenance - Temperature ................................................ 11

Sensor Storage ...................................................................................12

Troubleshooting ...............................................................................................12

Dissolved Oxygen Readings.............................................................12

Help ................................................................................................... 13

Error/Status messages ......................................................................13

Restore Default Calibration Values ................................................14

Probe Specifications......................................................................................... 15

Accessories / Parts List ....................................................................................16

Declaration of Conformity.............................................................................. 17

Recycling...........................................................................................................19

Contact Information........................................................................................20

Ordering and Technical Support.....................................................20

Service Information..........................................................................20

Appendix A-DO% Calibration Values........................................................... 21

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WA R R A N T Y

The YSI Professional OBOD®(ProOBODTM) probe and cable assembly is warranted

for two (2) years from date of purchase by the end user against defects in materials and

workmanship, exclusive of batteries and any damage caused by defective batteries.

ProOBOD sensor caps are wa rranted for one (1) year from date of purchase by t he end

user against defects in material and workmanship. ProOBOD systems (instrument

& cable/probe assemblies) are warranted for 90 days from date of purchase by the

end user against defects in material and workmanship when purchased by rental

agencies for rental purposes. Within the warranty period, YSI will repair or replace,

at its sole discretion, free of charge, any product that YSI determines to be covered

by this warranty.

To exercise this warranty, call your local YSI representative, or contact YSI

Customer Service in Yellow Springs, Ohio at +1 937 767-7241, 800-897-4151 or visit

www.YSI.com for a Product Return Form. Send the product and proof of purchase,

transportation prepaid, to the Authorized Service Center selected by YSI. Repair

or replacement will be made and the product returned, transportation prepaid.

Repaired or replaced products are warranted for the balance of the original warranty

period, or at least 90 days from date of repair or replacement.

LIMITATION OF WARRANTY

This Warranty does not apply to any YSI product damage or failure caused by:

Failure to install, operate or use the product in accordance with YSI’s written1.

instructions;

Abuse or misuse of the product;

Failure to maintain the product in accordance with YSI’s written instructions3.

or standard industry procedure;

Any improper repairs to the product;

Use by you of defective or improper components or parts in servicing or5.

repairing the product;

Modification of the product in any way not expressly authorized by YSI.6.

THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR

IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS

FOR A PARTICULAR PURPOSE. YSI’S LIABILITY UNDER THIS WARRANTY

IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT, AND THIS

SHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE

PRODUCT COVERED BY THIS WARRANTY. IN NO EVENT SHALL YSI BE

LIABLE FOR ANY SPECIAL, INDIRECT, INCIDENTAL OR CONSEQUENTIAL

DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BY

THIS WARRANTY.

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INTRODUCTION

Thank you for purchasing the YSI Professional Optical Biochemical Oxygen

Demand (ProOBOD) probe. The ProOBOD is designed for use with a YSI

ProODO® instrument. The probe will not work with any other YSI instrument.

This manual provides information on the ProOBOD probe only. For more

detailed instructions on how to operate the ProODO instrument, please refer to

the ProODO User Manual (www.ysi.com/ProODO).

The ProOBOD is designed to fit in a standard 300 ml BOD bottle as well as other

international BOD bottles, depending on the version of the probe. YSI offers

two probe versions:

ProOBOD USA (Japan), YSI item number 626400 - This probe fits in•

standard USA and Japanese style BOD bottles. A power supply for

USA and Japanese style AC outlets is included.

ProOBOD International, YSI item number 626401 - This probe fits•

in standard BOD bottles used outside the USA and Japan. A funnel

adapter for fitting in standard UK BOD bottles and a switching power

supply with 3 international outlet adapters are also included.

The ProOBOD sensor measures dissolved oxygen in water using lifetime

luminescence technology and uses a digital signal to send information between

the instrument and probe. The probe features a stirrer and motor to aid in

sample movement, to keep solids from settling at the bottom of the sample bottle,

and to speed up the response of the sensor. Key advantages of the ProOBOD’s

use of optical technology for DO measurement over traditional electrochemical

methods include the elimination of sensor flow dependence and sensor warm-

up time, greater stability, the ability to zero the sensor for more accurate

measurements at low dissolved oxygen levels, and the elimination of frequent

membrane/electrolyte changes and electrode servicing.

For more information on the ProODO instrument and product specification,

please visit www.ysi.com/ProODO or contact Technical Support at 800-897-

4151 (+1 937 767-7241).

Reading the entire manual before use is recommended for an overall

understanding of the probe’s features.

Important: The ProOBOD is designed for laboratory use, not

field use. It is not waterproof and should never be immersed

in a sample past the taper on the stem of the probe.

GETTING STARTED

Throughout the manual, the term “probe” refers to the end of the cable where

the sensor, stir motor, and stirrer are located. The term “sensor” refers to the

Optical Dissolved Oxygen sensing portion of the probe assembly and the term

“sensor cap” refers to the removable sensing cap that is replaced about once per

year (Figure 1).

Figure 1.

Unpacking the OBOD prOBe

Each ProOBOD probe and replacement OBOD sensor cap

includes an instruction sheet with important information

unique and specific to each individual sensing cap.

Carefully unpack the instrument and accessories and inspect for damage.

Compare received parts with items on the packing list. If any parts or materials

are damaged or missing, contact YSI Customer Service at 800-897-4151 (+1 937

767-7241) or the authorized YSI distributor from whom the instrument was

purchased.

OBOD Sensor Cap,

shown here removed

from probe

Probe

Stir paddle

Optical DO sensor

Stirrer toggle switch,

turns stir paddle

on and off

Temperature sensor

Remove the ProOBOD cable/probe assembly from the shipping container and

locate the instruction sheet that is included. This instruction sheet is important

because it contains the calibration coefficients for your sensor cap. After using

this sheet for general probe setup, be sure to store it in a safe place in case you

need to reload these calibration coefficients in the unlikely event that they are

ever deleted from the instrument and probe.

Note - A new cable/probe assembly already has a

sensor cap installed and the sensor cap coefficients

are preloaded into the probe at the factory.

Preparing the probe for the first time:

A new ProOBOD probe will have a clear protective cover placed over the1.

sensor cap to ensure the cap remains moist during shipment. Carefully

remove this clear cover from the end of the probe by pulling it straight off

the sensor. Save the clear cover along with the sponge in case you want to

use it later for long term storage of the probe.

Place the probe in a standard BOD bottle that contains a small amount of2.

clean water (approximately 40 ml). The sensor should not be immersed in

water but rather in the air above the water. The purpose is to create a 100%

water-saturated air environment for the sensor which is ideal for calibration

and storage when not in use.

It is important to always keep your sensor in a moist

environment so the sensor cap does not dry out. (See

Maintenance and Storage for more information.)

POBOD OPERATION

cOnnecting prOBe/caBle assemBly tO instrUment

To connect the cable, align the keys on the cable connector to the slots on the

instrument connector. Push together firmly, then twist the outer ring until it

locks into place (Figure 2). This MS-8 (Military Spec) connection is water-

proof.

Figure 2. Note the keyed connector. The cable and instrument connectors

can only be mated once the keyed sections are properly aligned.

cOnfigUring the pr o ODO instrUment

On the ProODO instrument, press the Probe key. Highlight DO and press

enter. Enabled allows you to enable or disable the DO sensor. Highlight Enabled

and press enter to activate or deactivate the dissolved oxygen sensor.

The DO Setup menu also displays the OBOD probe’s SW Version and Serial #.

This information is programmed into the probe at the factory and digitally sent

to the instrument when the cable is connected.

Within the DO Probe Setup menu, the Sensor Cap submenu allows you

to view and enter information specific to the sensor cap installed on the

probe. Highlight Sensor Cap and press enter to view the sensor cap’s Serial #,

Temperature Coefficient, and Sensor Cap Coefficients. This information is also

programmed into the probe at the factory and sent to the instrument when the

cable is connected.

The Sensor Cap Coefficients need to be updated when the sensor cap is replaced.

The frequency of sensor cap replacement is dependent on use. For example,

when the probe is powered on for approximately 4 hours per day, 5 days a week,

the sensor cap will need replaced about once per year. If the probe is powered

on more than this, the sensor cap will need replaced more often. To extend the

sensor cap’s usable life, turn the instrument off when not in use. For example,

turn the instrument off over night, but not between readings. The sensor cap

will also need replaced if it is damaged or cracked. See the Troubleshooting

section of this manual for instructions on how to determine if the sensor cap

needs replaced. See the Sensor Maintenance - Dissolved Oxygen section of

this manual or the instruction sheet included with the replacement sensor cap

for instructions on updating the Sensor Cap Coefficients. When updating the

Coefficients, the sensor cap serial # will be updated automatically based on your

entries.

The Temperature Coefficient should not be modified unless instructed by YSI

Technical Support.

For additional information about the ProODO’s menus and operation, please

refer to the ProODO User Manual.

pOwering the stirrer

To power the stirrer, locate the power supply that was included with the

ProOBOD. The power supply for the USA/Japan probe is labeled model number

6260. The power supply for the international probe is labeled model number

6261. Next, connect the power supply to the probe’s power connector and then

plug the power supply into an outlet. The international ProOBOD is shipped

with outlet adapters to connect the power supply to a variety of outlets. The

stirrer can be turned on and off with the toggle switch located at the top of the

probe (Figure 1).

caliBratiOn - DissOlveD Oxygen

The ProOBOD sensor is an optical luminescent sensor which has greater stability

and is less susceptible to calibration drift than traditional electrochemical

sensors. This increased stability means that the instrument may hold its

calibration for many months. However, for the highest data accuracy, YSI

recommends verifying the instrument’s calibration on a daily basis. To verify

the instrument’s calibration, place the sensor in its calibration environment and

check to see that the DO% is reading its calibration value based on the current

barometric pressure. Refer to Appendix A for a list of DO% calibration values

and corresponding barometric pressure readings.

The ProODO instrument with ProOBOD probe offers several options for

performing a dissolved oxygen calibration. For both ease of use and accuracy,

YSI recommends performing a DO % water-saturated air calibration as described

below. For information on other acceptable calibration techniques, including

how to perform a 2-point calibration with a zero solution, please refer to the

ProODO User Manual.

It is not necessary to calibrate in both % and mg/L or ppm.

Calibrating in % will simultaneously calibrate

mg/L and ppm and vice versa.

CALIBRATING DO% IN WATERSATURATED AIR:

1POINT CALIBRATION

Make sure there are no water droplets on the OBOD sensor cap or temperature

sensor and then place the probe in a BOD bottle that contains a small amount

of clean water (approximately 40 ml). The BOD bottle should be clean since

bacterial growth may consume or produce oxygen which would interfere with

the calibration. Make sure the DO and temperature sensors are not immersed in

water. The purpose is to create a 100% water-saturated air environment. Next,

wait approximately 5 to 10 minutes or until the DO and temperature readings

are stable. This will allow for the air in the BOD bottle to become completely

water-saturated. The design of the probe ensures venting to the atmosphere

when placed in a typical BOD bottle which is required to create the 100% water-

saturated air calibration environment.

Press the Calibration key on the ProODO instrument. Highlight DO and

press enter. Highlight DO % and press enter to confirm.

The instrument will use the reading from the

internal barometer during calibration and will

display this value in brackets at the top of the

display. Highlight the barometer value and

press enter to adjust it if needed. Note - the

barometer should be reading “true” barometric

pressure. If the barometer reading is incorrect,

perform a barometer calibration. See the

Barometer section in the ProODO User Manual

for more information on “true” barometric

pressure and on how to perform a barometer

calibration. If the barometer reading is acceptable, there is no need to change it

or perform a barometer calibration.

Wait for the temperature and DO% values under “Actual Readings” to stabilize,

then highlight Accept Calibration and press enter to calibrate. If User Field 1

or 2 are enabled, you will be prompted to select the fields and then press Cal

to complete the calibration. The message line at the bottom of the screen will

display “Calibrating Channel...” followed by “Calibration Successful”. Press Esc

to cancel the calibration.

itemperatUre

All ProOBOD probes have built-in temperature sensors. Temperature calibration

is not required nor is it available. To set the units, press the Probe key on

the ProODO instrument and select Display. Highlight Temperature and press

enter. Highlight the desired temperature units of °F,°C, or Kand press enter to

confirm the selection.

taking measUrements

Important: The ProOBOD is designed for laboratory use, not

field use. It is not waterproof and should never be immersed

in a sample past the taper on the stem of the probe

To take readings, insert the probe into the BOD sample bottle. The DO and1.

temperature sensors should be immersed in the sample.

Turn the probe stirrer on with the toggle switch located on the top of the2.

probe (Figure 1). The stirrer helps keep solids from settling at the bottom

of the sample bottle and quickens the sensor’s response.

Allow the temperature readings to stabilize and wait approximately 20-253.

seconds for the DO readings to stabilize.

Log One Sample will be highlighted on the ProODO instrument when it is4.

in Run mode. Press Enter to open a logging submenu. Highlight Sites or

Folders and press Enter to select a site or folder to log the sample to.

Once the Site and/or Folder name is selected, highlight5. Log Now and press

Enter. The instrument will confirm that the data point was successfully

logged.

If you would like to log at a specific interval vs. logging one sample at a time6.

or vice versa, press the System key, then highlight Logging and press

Enter. Select Continuous Mode and adjust the time interval if necessary.

On the Run screen, the option to log will change from Log One Sample to

Start Logging based on the time interval entered in the Logging Menu.

Note – When utilizing the Manual sampling mode, the

Continuous Logging Interval must be set to 10 seconds or

greater. For additional information on logging data and the

sampling mode options, refer to the ProODO User manual.

MAINTENANCE AND STORAGE

This section describes the proper procedures for care, maintenance and storage

of the ProOBOD probe.

Important: Do not attempt to access the probe motor

assembly or open the probe body. Doing so will void

any remaining warranty. The probe body should only

be opened by a YSI Authorized Service Center.

UpDating instrUment anD prOBe firmware

The instrument and probe firmware can be updated via www.ysi.com/software.

There you will find the new firmware files and instructions on how to update the

instrument and/or probe.

sensOr maintenance - DissOlveD Oxygen

CLEANING THE OBOD SENSOR CAP

The Sensor Cap should be kept clean since some types of fouling may consume

or produce oxygen which could affect the dissolved oxygen measurements. To

clean the Sensor Cap, gently wipe away any fouling with a lens cleaning tissue

that has been moistened with water. Do not use a coarse towel or cloth or organic

solvents to clean the Sensor Cap. Using a coarse towel or organic solvent to clean

the Sensor Cap may cause permanent damage to the cap. For example, alcohol

will dissolve the outer paint layer and other organic solvents will likely dissolve

the dye in the cap. If the sensor cap is damaged, it must be replaced.

OBOD SENSOR CAP REPLACEMENT