Advanced Instruments Anoxomat III User manual

Anoxomat® III
Jar System
ServiceGuide
AN3007EN Rev 0

2
Anoxomat®III Service Guide
Copyright and trademarks
© 2018 Advanced Instruments
This guide is copyrighted by Advanced Instruments with all rights reserved. Under copyright laws, this guide may not be
reproduced in any form, in whole or part, without the prior written consent of Advanced Instruments. The following are
trademarks of Advanced Instruments:
• Anoxomat
• Advanced
• Hot-Line™
Employees of Advanced Instruments have reviewed this guide thoroughly. Although material contained within is believed
reliable, the accuracy and completeness are not guaranteed or warranted, and are not intended to be representations or
warranties concerning the product described. Screenshots and images included in this guide are for purposes of example
only and may not be an exact match with the screens you see.

Contents
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Anoxomat®III Service Guide
Contents
Contents.............................................................................3
Contacting Advanced Instruments..............................4
Supplies, jars, and accessories .....................................5
Safe use..............................................................................6
Foreword.......................................................................... 10
Chapter 1
Installation and setup .................................................... 11
1.1 Location requirements.....................................................................11
Adequate space ............................................................................11
Electric outlet availability........................................................... 11
1.2 Additional items needed for installation...................................11
1.3 Carton contents..................................................................................11
Accessories and optional parts................................................11
Unpacking ........................................................................................11
1.4 Installing the gas supply ................................................................12
Regulator requirements .............................................................12
Connecting the gas supply to a 220V system .................. 12
Rear connector panel..................................................................13
This..................................................................................................... 13
Connecting the gas supply to a 100V or 110V system ...13
1.5 Install the recipe printer (optional)............................................14
1.6 Connect to a computer or network (optional)......................14
1.7 Power up the instrument ...............................................................14
1.8 Set up System Language...............................................................15
1.9 How to input data.............................................................................15
1.10 Setting up the instrument .............................................................15
Set up gas connection(s) ..........................................................15
Setup date and time....................................................................15
Exit Setup........................................................................................15
1.11 Standard gas mixture......................................................................16
1.12 Registration setup (optional).......................................................16
Jar setup .......................................................................................... 17
Register users ................................................................................ 17
1.13 Activating the printer (optional).................................................18
1.14 Activating the data interface (optional) ..................................18
Connecting to a network...........................................................18
Chapter 2
Maintenance and Service............................................. 19
2.1 Routine maintenance ......................................................................19
2.2 Maintaining jars..................................................................................19
2.3 Cleaning and disinfecting jars.....................................................20
2.4 Expected jar service life................................................................20
2.5 Maintaining valves ...........................................................................20
2.6 Cleaning valves.................................................................................20
2.7 Cleaning and lubricating lid couplings..................................... 21
2.8 Options/accessories ........................................................................21
Chapter 3
Troubleshooting.............................................................22
3.1 Service and maintenance cautions ...........................................22
3.2 Obtaining service.............................................................................23
3.3 To return an instrument for repair or replacement.............23
3.4 Troubleshooting checks ................................................................23
3.5 Process report errors......................................................................23
3.6 Hardware test program .................................................................23
3.7 Fuse replacement ............................................................................24
3.8 Catalyst errors...................................................................................24
3.9 Jar closed error ................................................................................25
3.10 Jar connection errors.....................................................................25
3.11 Gas connection errors....................................................................26
3.12 Pump time exceeded error ..........................................................26
3.13 Fill time exceeded errors ..............................................................26
3.14 Jar leak test errors........................................................................... 27
3.15 Jar seal test errors........................................................................... 27
3.16 Pressure too low errors ................................................................. 27
3.17 Pressure too high errors................................................................28
3.18 Indicator strip errors.......................................................................28
3.19 System errors ....................................................................................28
Appendix A
Product specifications.................................................. 31
Appendix B
Regulatory notices ........................................................32
Appendix C
Product disposal and recycling..................................33
Appendix D
System Schematic ........................................................ 34
Reproduction of any kind is prohibited except with
the specific permission of Advanced Instruments. ...
34

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Anoxomat®III Service Guide
Contacting Advanced Instruments
General inquiries
• US or Canada
• Toll free phone: [phone] from 8:00 AM – 4:30 PM Eastern time
• Fax: [phone]
• Outside the US or Canada:
• Phone: [phone]
• Fax: [phone]
• Email: [e-mail]
• Web: aicompanies.com
Customer Service/Orders
To order supplies for the Anoxomat, contact the Customer Service department.
• US or Canada
• Toll free phone: [phone] from 8:00 AM – 4:30 PM Eastern time
• Fax: [phone]
• Outside the US or Canada:
• Phone: [phone]
• Fax: [phone]
• Email: [e-mail]
Hot-Line™ Technical Service
For any questions or problems related to operation or service, contact the Hot-Line Technical Service department.
• US or Canada
• Toll free phone: [phone] from 8:00 AM – 4:30 PM Eastern time; after normal business hours, follow prompts
• Fax: [phone]
• Outside the US or Canada:
• Phone: [phone]
• Fax: [phone]
• Email: [e-mail]
NOTE: When contacting Advanced Instruments for technical support, please be ready to provide the service technician with
the model and the serial number of the instrument, as well as a general description of the problem. It may be
necessary to perform diagnostic checks during the service call, so please contact us from a telephone that allows you
immediate access to the instrument. A service technician may verbally assist you with making minor repairs, may
provide you with information regarding recommended parts or part numbers, and/or may issue you a Return
Merchandise Authorization (RMA) to ship the instrument to us for factory repairs. The RMA procedure is as follows:
1. Contact Advanced Instruments to obtain an RMA.
2. Pack and ship the instrument in its original carton. Do not include instrument supply items.
3. Pre-pay shipment to the factory. Advanced Instruments cannot accept collect shipments.
4. Insure the shipment or accept the risk of potential damage.

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Anoxomat®III Service Guide
Supplies, jars, and accessories
To order supplies, standards, and controls, contact the Advanced Instruments Customer Service Department (see
Contacting Advanced Instruments).
Part Description
Instruments
100V 110V 220V
ANX1J1G-JP ANX1J1G-1 ANX1J1G-2 Anoxomat, 1 Jar and 1 Gas Connection
ANX1J2G-JP ANX1J2G-1 ANX1J2G-2 Anoxomat, 1 Jar and 2 Gas Connection
ANX1J3G-JP ANX1J3G-1 ANX1J3G-2 Anoxomat, 1 Jar and 3 Gas Connection
ANX2J1G-JP ANX2J1G-1 ANX2J1G-2 Anoxomat, 2 Jar and 1 Gas Connection
ANX2J2G-JP ANX2J2G-1 ANX2J2G-2 Anoxomat, 2 Jar and 2 Gas Connection
ANX2J3G-JP ANX2J3G-1 ANX2J3G-2 Anoxomat, 2 Jar and 3 Gas Connection
ANX3J1G-JP ANX3J1G-1 ANX3J1G-2 Anoxomat, 3 Jar and 1 Gas Connection
ANX3J2G-JP ANX3J2G-1 ANX3J2G-2 Anoxomat, 3 Jar and 2 Gas Connection
ANX4J1G-JP ANX4J1G-1 ANX4J1G-2 Anoxomat, 4 Jar and 1 Gas Connection
Part Description
Jars
AJ9025
Holds one stack of 12 Petri dishes, 9-10 cm
diameter
Holds three Microtiter plates, 13 x 9 cm
Holds one stack of 12 Petri dishes, 15 cm
diameter
AJ9049 Holds two stacks of 6 Petri dishes, 9-10 cm
diameter
AJ9050 Holds two stacks of 12 Petri dishes, 9-10 cm
diameter
AJ9028 Holds three stacks of 12 Petri dishes, 9-10 cm
diameter
Petri dish holders
PH 1040 Holds 12 Petri dishes, 9-10 cm diameter
PH 1050 Holds three stacks of 12 Petri dishes, 10 cm
diameter
PH 1060 Holds 6 Petri dishes, 9-10 cm diameter
PH 1070 Holds 10 Petri dishes, 14.5 cm diameter
PH 1080 Holds three stacks of 12 Petri dishes, 6 cm
diameter
PH 1090 Holds one stack of microtiter plates, 13 x 9 cm
diameter
TH0000 For use with AJ9050, holds 14 (16 mm tubes)
Part Description
Anoxomat accessories and supplies
AN2GC Additional gas connection
AN2JC Additional jar connection
AN2BCS Barcode scanner
AN2ISC1 Registration input screen
AN2DI Data interface
AN2PPR Pre-programmed recipes
AN2TT Track and trace package
AN2UPF User programming function
AN2TP1 Recipe printer, thermal paper or medical grade
paper
AN2TP3 Dot matrix printer for plain paper
MT2000 Jar maintenance toolkit
MT2001 Refill for jar maintenance toolkit
AN3146 Palladox disposable sachet (box of 40)

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Anoxomat®III Service Guide
General cautions
• Only power this instrument with the source specified
on the product electrical ratings label. Refer to the
installation instructions.
• If the power cord provided is replaced for any reason
or if an alternate cord is used, the cord must be
approved for use in the local country. The power cord
must be approved for the product’s listed operating
voltage and must be rated at least 20% greater than
the volt-ampere (VA) ratings marked on the product’s
electrical ratings label. The cord end that connects to
the product must have an IEC 60320 connector.
• Plug the product into an approved grounded electrical
outlet. Exposure to AC voltage could lead to electric
shock, burn, or electrocution. Please be aware of any
exposure of AC voltage in the power cord.
• Do not disable the power cord grounding pin.
• If an extension cord or power strip is used, make sure
that the cord or strip is rated for the product and that
the total ampere ratings of all products plugged into
the extension cord or strip do not exceed 80% of the
cord or strip rating limit.
• Route power cords so that they will not be walked on,
tripped on, or pinched by items placed upon or against
them. Pay particular attention to the plug, electrical
outlet, and the point where the cord exits the product.
• Do not pull on cords and cables; grasp the
corresponding connector to unplug it.
• Do not install or use this product in any area subject to
extreme short-term temperature variations, or in
locations that exceed the specified operating
environment temperatures.
• Never use this product in a wet area.
• To avoid injury or fire hazard, do not operate this
product in an explosive atmosphere.
• Do not install or use the product on an unstable,
non-level work surface.
• Do not operate this product with the covers removed
or unsecured.
Safe use
WARNING: To reduce the risk of bodily injury, electric shock, fire, and damage to the instrument, please read and
observe all precautions in this guide.
Only operate this product in accordance with the equipment design, operating instructions, and manufacturer
recommendations; to do otherwise may impair the instrument function or create a safety hazard.
Do not attempt to perform electrical work if you are not fully qualified. This guide is not a substitute for electrical training.

Safe use
7
Anoxomat®III Service Guide
Service and maintenance cautions
• Unplug the power cord before opening or removing
the covers; otherwise, you may be exposed to electric
shock, excessive temperatures, or mechanical hazards.
• Never restrict airflow into or out of the product. Check
the air vents for blockages periodically.
• Wipe the exterior of the product with a soft, damp
cloth as needed. Using cleaning products other than
those specified may discolor or damage the finish.
• A discharge of static electricity from contact with the
human body or another conductor may damage
system boards or static sensitive devices. Never
perform internal maintenance without following
recommended static protection procedures.
• The product is equipped with operator accessible
fuses. If a fuse blows, it may be due to a power surge
or the failure of a component. Replace the fuse one
time only. If the fuse blows a second time, it is
probably due to the failure of a component part and
all additional service requirements should be referred
to a qualified technician. Always replace the fuse with
one of the same rating, voltage, and type. Never
replace the fuse with one of a higher current rating.
• If the product requires service for any of the following
reasons, unplug the product from the electrical outlet
and request assistance from a qualified service
technician:
• The power cord, extension cord, power strip, or
power input module is damaged.
• Liquid has been spilled into the interior of the
product.
• A foreign object has fallen into the product.
• The product has been dropped or damaged by a
falling object.
• There are noticeable signs of overheating or a
burning odor.
• The product does not operate normally when you
follow the operating procedures.
• The main supply fuse(s) or any internal fuse(s)
continually fail.
• When servicing the product, use only factory-specified
parts.
• To avoid injury or fire hazard, do not operate this
product in an explosive atmosphere.
• Use caution and appropriate personal protective
equipment (PPE) if you must put your hands inside the
osmometer. Placing hands into the opening could
result in skin punctures or other injuries.
Hazardous material warnings
NOTICE: Autoclaving is not permitted.
• WARNING: Handle all biohazardous materials
according to established good laboratory practices
and follow your institution’s exposure control plan.
Persons handling blood and body fluid samples must
be trained in blood-borne hazards and should observe
Universal Precautions for Preventing Transmission of
Bloodborne Infections as defined by the Centers for
Disease Control and Prevention (CDC). The Universal
Precautions guideline provides infection control by
treating all blood and body fluids as if known to be
infectious. Use personal protective equipment such as
gloves, gowns, etc., to prevent exposure. Store
biohazardous materials in regulated waste containers
and dispose of these materials in a safe and
acceptable manner that is in compliance with all
country, state, and local requirements.
• WARNING: When returning this product for service, or
shipping this product to a second location, remove all
hazardous specimens and decontaminate the product
before packaging it for shipment. If the product
cannot be decontaminated, consult with your shipping
agent for instructions on appropriate packaging and
marking.
• WARNING: If a biohazardous material is spilled on or
inside the equipment, decontaminate the equipment
using a 1% bleach solution or as outlined by those
policies and procedures established within your
institution.

Safe use
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Anoxomat®III Service Guide
Symbol conventions
The list of symbols below is for reference only. Some symbols shown below might not appear on the instrument or in the
accompanying documentation.
Symbol Description
This symbol alerts the user to the presence of important operating and maintenance (servicing) instructions.
This symbol alerts the user to the presence of uninsulated dangerous voltage inside the enclosure that may
constitute risk of electric shock to persons.
This symbol alerts the user to the presence of internal components that could be damaged by static electricity.
This symbol alerts the user to the presence of biological substances that may pose a threat to the health of living
organisms, primarily that of humans.
This symbol indicates potential puncture hazards; users keep hands clear.
This symbol alerts the user to the presence of a specific component that could be damaged by static electricity.
This symbol indicates the presence of alternating current (AC).
This symbol indicates the presence of a fuse.
This symbol indicates the presence of protective earth ground.
This symbol indicates the power is ON.
This symbol indicates the power is OFF.

Safe use
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Anoxomat®III Service Guide
FCC Statement
WARNING: Changes or modifications to this unit not
expressly approved by the party responsible for
compliance could void the user’s authority to operate the
equipment.
This equipment has been tested and found to comply
with the limits for a Class A digital device, pursuant to
Part 15 of the FCC Rules. These limits are designed to
provide reasonable protection against harmful
interference when the equipment is operated in a
commercial environment. This equipment generates,
uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction
manual, may cause harmful interference to radio
communications. Operation of this equipment in a
residential area is likely to cause harmful interference in
which case the user will be required to correct the
interference at his own expense.
Industry Canada Statement
CAUTION: Any changes or modifications not expressly
approved by the party responsible for compliance could
void the user’s authority to operate the equipment.
ATTENTION: Tout changement ou modification non
expressément approuvé par la partie responsable de la
conformité pourrait annuler l’autorisation de l’utilisateur
d’utiliser l’équipement.
This device complies with Industry Canada license-
exempt RSS standard(s). Operation is subject to the
following two conditions: (1) This device may not cause
interference, and (2) this device must accept any
interference, including interference that may cause
undesired operation of the device.
Le présent appareil est conforme aux CNR d’Industrie
Canada applicables aux appareils radio exempts de
licence. L’exploitation est autorisée aux deux conditions
suivantes: (1) l’appareil ne doit pas produire de
brouillage, et (2) l’utilisateur de l’appareil doit accepter
tout brouillage radioélectrique subi, même si le
brouillage est susceptible d’en compromettre le
fonctionnement.

10
Anoxomat®III Service Guide
Foreword
Intended use
The Anoxomat® uses the widely-known McIntosh and Fildes system of evacuation and replacement to create anaerobic
environments. The Anoxomat quickly creates anaerobic, microaerophilic, or capnophilic and environments to cultivate
bacteria in jars using a reliable automated evacuation/replacement method. The Anoxomat can create exact, repeatable
conditions with gas mixtures within 0.5% of the required value.
The Anoxomat is designed to cultivate oxygen-sensitive organisms in anaerobic jars using a system that reliably sustains
environments for proper growth. The Anoxomat is useful to the following disciplines:
• Microbiological and bacteriological research
• Clinical microbiology laboratories
• Medical research
• Biotechnology research and manufacturing
• Pharmaceutical research and manufacturing
• Food safety microbial testing
• Environmental monitoring
• Academic research
• Industrial applications

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Anoxomat®III Service Guide
1.3 Carton contents
Quantity Description
1 Anoxomat III
1 Parts package
• Gas hose(s) and fittings or gas tube(s)
• Jar tube test plug
• Soft-touch pen
1 USB stick containing
• User’s guide
• Data Interface spreadsheet template
1 AC line cord
Accessories and optional parts
• Jar
• Petri dish holder
• Palladox™ catalyst (disposable sachet)
• 1 Recipe printer (optional)
• 1 Barcode scanner (optional)
Unpacking
1. Carefully unpack the carton and inspect the contents
for damage.
Save the shipping material in case you need to
transport the instrument.
2. Use the packing list to verify you have all items.
NOTE: Notify your receiving department immediately if
items are missing. Advanced Instruments is
responsible for items reported missing within 10
days of a shipment’s arrival.
3. If an item is damaged, save the shipping material for
inspection by the insurer.
4. Notify the dealer and Advanced Instruments within
24 hours for your warranty and insurance to apply.
Chapter 1
Installation and setup
NOTICE: In order to set up your instrument
properly, it is important that you read and
follow the steps in this section.
1.1 Location requirements
When choosing a location for your Anoxomat, make sure
to meet the following criteria.
Adequate space
• Instrument dimensions:
12 × 9 × 13 inches (30.5 × 23 × 33 cm).
• Keep workplace free of debris and allow 6 inches
(15 cm) of clear space on each side of the instrument.
• Allow space for jars and accessories, such as a printer.
Electric outlet availability
WARNING: Your instrument needs to operate
within five feet of a properly-grounded,
three-prong electrical outlet capable of
continuously supplying 2 amperes at 220V and
5 amperes at 100-110V. If the instrument is not
grounded properly, its operation may be
impaired and a safety hazard may exist.
Therefore, verify the AC voltage that your
instrument operates at, be sure to test the
outlet, and record the results before operating
your instrument.
1.2 Additional items needed for installation
• Adjustable wrench to tighten the dual stage regulator.
• 5/8 in & 11/16 in wrenches to tighten the hose/fitting
gas connection(s).
• Gas cylinder containing appropriate anaerobic mixture.
• Dual stage regulator. See Installing the gas supply on
page12.

Chapter 1 Installation and setup
12
Anoxomat®III Service Guide
5. Have the transportation company inspect items,
complete a “Report of Concealed Damage,” and file
your claim.
6. Notify Advanced Instruments immediately for repair
or replacement.
7. Complete the online warranty card at:
aicompanies.com/warranty
Anoxomat III and accessories
1.4 Installing the gas supply
To use the Anoxomat, you'll need to connect at least one
gas cylinder with a standard gas mixture used for
anaerobic as well as for microaerophilic bacteria
cultivation (see Standard gas mixture on page16).
NOTE: Before you begin, make sure the gas cylinder valve
is o until the gas supply is fully installed.
Caution: Improper connections may cause the instrument
to malfunction.
Regulator requirements
• Reverse thread for mixed gases
• Dual-stage
• Reads 0–3.45 bar (0–50 psi)
• Required connections:
– 220V systems: 8 mm (5/16 in.) hose-barb fitting
– 100 or 110V systems: 1/4 in. NPT port
• Capacity max. 5 m3/hr (176 ft3/hr)
• Regulator shall comply with safety standards
• Gas cylinder equipped with a CGA350 fitting on tank
• Working pressure: 1.75 bar ± .15 bar 25.5 ± 2 psi
• 220V system: Gas tube is 8 mm (5/16 in.) inner
diameter; working pressure of 15 bar (218 psi).
• 100V or 110V system: Gas hose is 8 mm (5/16 in.) inner
diameter; working pressure of 172 bar (2500 psi) or
less.
Connecting the gas supply to a 220V system
See Installing the gas supply before you begin.
1. Mount the regulator on the gas cylinder
using the adjustable wrench.
2. Tighten the regulator per manufacturer’s
recommendations to avoid gas leakage.
3. Set the regulator's gas pressure to zero.
4. Using the provided tube clamps on each end,
connect the gas tube to the regulator and the gas
input #1 (see the Rear connector panel).
5. Tighten the gas tube clamps.

Chapter 1 Installation and setup
13
Anoxomat®III Service Guide
Rear connector panel
This image is for reference only. Your Anoxomat system
may have dierent connections.
This
6. Open the gas cylinder’s main supply and set the
regulator gas pressure to 1.75 ± .15 bar
(25.5 psi ± 2 psi).
7. Repeat for each gas connection.
Connecting the gas supply to a 100V or 110V system
See Installing the gas supply before you begin.
1. Mount the regulator on the gas cylinder using the
adjustable wrench.
2. Tighten the regulator per manufacturer's
recommendations to avoid gas leakage.
3. Set the regulator's gas pressure to zero.
4. Hand-tighten the gas fitting into the regulator.
Use a 5/8 in. wrench to fully tighten the fitting.
5. Hand-tighten the metal gas hose to the gas fitting.
Use an 11/16 in. wrench to fully tighten the hose.
Hand-tighten the other end of the hose to the gas fitting
on the back of the Anoxomat. Use an 11/16 in. wrench to
tighten the hose.

Chapter 1 Installation and setup
14
Anoxomat®III Service Guide
6. Slowly open the gas cylinder valve and set the gas
regulator to 1.75 bar ± .15 bar 25.5 ± 2 psi.
7. Repeat for each gas connection.
1.5 Install the recipe printer (optional)
1. Connect the printer cable to the RS232 socket on the
rear panel of the Anoxomat.
2. Install the roll of printer paper.
3. Connect the printer's power cord to the Anoxomat's
printer power connector.
See Activating the printer (optional) on page18.
NOTE: The red protection label contains important
information and setup codes for the Key Operator,
and should be stored in a safe place. See Figure 1.1.
1.6 Connect to a computer or network
(optional)
If your Anoxomat includes a data interface to a computer
or network:
• Use a “cross-link” Ethernet cable to connect the
Anoxomat directly to the network card of the
computer.
• Use a standard patch cable to connect to a network.
See instructions for Activating the data interface
(optional) on page18.
1.7 Power up the instrument
1. Connect the power cord to the Anoxomat and into a
power outlet.
2. Remove and set aside the red protection label
(Figure 1.1)from the touchscreen.
NOTE: Keep the red label in a safe place as it contains
configuration access codes.
Figure 1.1: Red touchscreen protection label
3. Turn on the power switch and wait for the Main
operation screen to display.
4. The Anoxomat is equipped with a touchscreen that
responds to the soft-touch pen supplied with the
instrument or the tips of your fingers. The
touchscreen may not respond well if you press with
the flat of your finger. Never use sharp objects, pens,
pencils, or anything that would leave a mark on the
screen.

Chapter 1 Installation and setup
15
Anoxomat®III Service Guide
1.8 Set up System Language
Your Anoxomat contains multiple languages. The
supported languages are: Chinese, Dutch, French,
German, Japanese, Korean, Russian, and Spanish.
1. To set the language, press Language on the Setup
screen.
2. Select the language.
3. Press Close and reboot the system.
The system displays in the selected language.
1.9 How to input data
When programming or changing settings in the
Anoxomat, you must enter data on an input line using an
onscreen keyboard. Using the soft-touch pen or your
fingernail, tap in the input line to position a blinking
cursor, and a keyboard appears.
1.10 Setting up the instrument
This section is restricted for use by the Supervisor, who
is required to enter the Set-up Code from the red label
(Figure 1.1)before defining or changing settings.
1. Press Setup. The setup code screen displays with
keyboard.
2. Enter the Setup Code from the red label (Figure 1.1)
and press Ok. The main Setup screen displays.
Set up gas connection(s)
The Anoxomat allows a maximum of three gas supply
connections. For each gas connection, you need to
specify the gas mixture on the Setup screen.
For Gas #1, select the gas composition of your gas from
the corresponding pull-down menu.
Specify the gas composition for each gas supply.
See Standard gas mixture on page16.
If your gas composition is not available from the pull-
down list, you can define the mixture by selecting
Other gas-composition. This screen appears:
Enter the required percentages in each field and press
Close to exit the screen and save changes.
If the second or third gas connection is not used, leave
the field empty or select None.
Setup date and time
If your Anoxomat is equipped with a printer and/or is
using a data interface, the date and time of a process
appears on the printout or in the database.
To set the date and time, press Date/time on the Setup
screen. Enter the date as DD-MM-YYYY (e.g., 30-04-2010
= April 30, 2010). Based on 24-hour clock, set the time in
HHMM (e.g., 09-07 = 9:07 AM).
Exit Setup
Press Close to return to the Main Operation Screen.

Chapter 1 Installation and setup
16
Anoxomat®III Service Guide
Application Gas Composition
O2CO2H2N2
Primary gas supply (alternative 1) – 10.0% 10.0% 80.0%
Standard microaerophilic recipe 6.0% 7.1% 7.1% 79.8%
Standard anaerobic recipe 0.0% 10.0% 10.0% 80.0%
Primary gas supply (alternative 2) ˜ 10% 5% 85%
Standard microaerophilic recipe 6.0% 7.1% 3.6% 83.3%
Standard anaerobic recipe 0.0% 10.0% 5.0% 85.0%
Table 1.1 Gas supply mixtures and jar composition results
1.11 Standard gas mixture
The standard gas mixture could have a composition as
specified in the line “Primary gas supply” in Table 1.1,
along with the resulting jar mixture using the standard
recipes.
The Anoxomat processes the recipes independent of
the gases or gas mixtures used.
You must use the primary gas connection (gas #1) for
your anaerobic gas mixture cylinder. Normally, the
secondary gas connection (gas #2) is used for the 100%
CO cylinder, and the third gas connection (gas #3) is
used for the H mixture cylinder.
WARNING: Gas mixtures with hydrogen greater
than 10% are considered flammable. Gas mixtures
with concentrations of oxygen above the normal 21%
atmospheric significantly increases the risk of ignition
and fire. Never use such hazardous gas mixtures with the
Anoxomat.
1.12 Registration setup (optional)
If you purchased the optional Registration feature, you
receive a setup code that allows you to configure your
Anoxomat to record additional information on how
samples are processed, and who processed them. The
details are stored in the Anoxomat, can be printed with
the optional recipe printer, and can be transferred to a
computer using the optional data interface.
1. On the Setup screen, press Registration. The Unlock
Registration screen appears.
2. Enter the Registration Code from the red label
(Figure 1.1)and press Ok.

Chapter 1 Installation and setup
17
Anoxomat®III Service Guide
3. The main Registration screen appears.
4. Using the keyboard, enter the Lab ID and Dept. ID.
Jar setup
If you configure the jar setup, the user is prompted to
input data (e.g., Jar ID, contents of jars, job reference,
etc.) before each recipe is started.
1. On the Registration screen, press Jar Setup.
The Jar Setup screen displays a list of options and
corresponding checkboxes.
2. Using the soft-touch pen or your fingernail, select the
fields you want to include by tapping each box to
place a checkmark. If you checked a box by mistake,
tap again to remove the checkmark.
3. If you select the Authorized by checkbox, a blank line
for signature/name authorization appears on the
printout.
4. Press Close to save your settings and exit to the
Registration screen.
For each field selected, the user can enter the respective
information for each jar connected. If no information is
entered, a blank line appears on the printout. However, if
the Required checkbox is selected, the user must enter
the information.
NOTE: The ID separator is used when multiple patient
plates are scanned and processed in the jar.
Register users
To limit access to the Anoxomat, user IDs can be
established to identify the technician before every recipe
is run.
1. On the Registration screen, press Register Users.
2. In the “Add user” section of the screen, enter the user’s
full name as you want it to appear on the printout and
in the database.
3. Enter the user code for the user. The user code does
not appear anywhere but must be entered each time a
recipe is started.
NOTE: As long as even a single user has been registered
to use the Anoxomat, the Anoxomat cannot be
operated without entering a correct user code.
4. Press Submit to add the name and code to the list of
registered users.
5. Repeat steps 2–4 for each registered user.
6. To delete a registered user, select the entry from the
Registered Users list and press Remove.
7. Press Close to save your settings and exit to the
Registration screen. When you're finished configuring
Jar Setup and Register Users, press Close.

Chapter 1 Installation and setup
18
Anoxomat®III Service Guide
1.13 Activating the printer (optional)
If the optional thermal or dot matrix printer is installed,
you must activate the printer before use.
NOTE: Only printers supplied by Advanced Instruments
are compatible with the Anoxomat system.
1. On the Setup screen, press Printer. The Connect
Printer screen displays with keyboard.
2. Enter the Printer Code from the red label (Figure 1.1)
and press Ok. The Printer screen appears.
3. Choose the correct printer type and press Test Printer.
4. Select Printout Automatically to print the Anoxomat
recipe cycle information without prompting the user
at the end of each cycle.
1.14 Activating the data interface (optional)
If you purchased the optional Data Interface feature, you
need to setup and activate the software before you can
transfer data to a network or PC.
1. On the Setup screen, press Interface.
2. Enter the Data Interface Code from the red label
(Figure 1.1)and press Ok. The Network Settings screen
appears.
Connecting to a network
Use a standard patch cable (Ethernet Cable) to connect
to a network. Select Obtain an IP address automatically
and reboot the Anoxomat. The IP Address and Subnet
Mask appears in the network settings
Connecting directly to a PC
Use a “cross-link” Ethernet cable to connect the
Anoxomat directly to the network card of the PC.
Contact your system administrator for a range of IP
addresses to be used and enter a fixed IP address .
Set the IP address of the network card in your PC within
the same range. Reboot the Anoxomat to activate the
connection.

19
Anoxomat®III Service Guide
Chapter 2
Maintenance and Service
If you need assistance with service procedures, contact
Hot-Line Technical Service (see Contacting Advanced
Instruments). For eciency, have the model and serial
number from the bottom of the instrument available
when you speak to a technician.
If you need to send the Anoxomat for servicing, a
Hot-Line™ Technical Service representative will provide
you with an RMA number. Units are not accepted for
service without an RMA number.
WARNING: Always unplug the power cord
before opening or removing the instrument
cover. You could be exposed to electric shock,
excessive temperatures, or mechanical
hazards.
NOTICE: A discharge of static electricity from
contact with the human body or other
conductor can damage system boards or
static-sensitive devices. Never perform
internal maintenance without following
recommended electrostatic discharge (ESD)
protection procedures.
2.1 Routine maintenance
This chapter describes the procedure for maintaining
your instrument. Make sure the Anoxomat is unplugged
when you clean or maintain it.
Maintenance must be performed on a regular basis to
conserve the Anoxomat's performance. Follow the
recommendations set forth below to properly maintain
the instrument:
• Never allow jar tubes to hang loose when unattached,
as the system always tests all jar tubes to check for
attached jars. Instead, place unused tubes in the
holder on the side of the system
• Check the condition of the jar tube fittings and replace
all items that are visibly worn.
• The housing of the Anoxomat can be cleaned with a
damp cloth only (never too wet). Isopropyl alcohol or
diluted bleach may be used (see Maintaining Jars).
• Visual checks for cracks and bends should be made
regularly on all tubing and external cabling. In case
of damage, new tubing and external cabling should
be ordered and replaced immediately. Use only
replacement parts from Advanced Instruments or an
authorized distributor (see Replacement Parts).
• Install current software updates on the instrument.
2.2 Maintaining jars
It is recommended that Anoxomat jars be stored
between 0˚C and 50˚C.
It is advisable to perform the following maintenance
every three months on the jars:
• Spray a little PTFE1spray in the snap-shut coupling to
keep the coupling lubricated.
• Clean and apply acid-free petroleum jelly on the
rubber O-ring in the lid of the standard jars only. Clean
and leave the seal dry (do not apply petroleum jelly )
in the ergonomic jar lids.
To be certain that the jars are maintained correctly and
regularly, a small box with necessities to maintain all jars
working with the Anoxomat (standard jars or Ergonomic
jars) is available. This kit includes 10 replacement snap
shut couplings (these items cannot be cleaned, but can
be lubricated with Teflon spray), tools, cleanser and
lubricant. The Jar Maintenance Toolkit (Product No.
MT2000) is available from Anoxomat.
1 Polytetrafluoroethylene

Chapter 2 Maintenance and Service
20
Anoxomat®III Service Guide
2.3 Cleaning and disinfecting jars
The Mart Jars are never to be cleaned with bleach
alcohol, and are not to be exposed to temperatures
greater than 80˚C.
NOTICE: Autoclaving is not permitted.
Advanced Instruments recommends using a general dis-
infectant used for lab equipment that does not contain
bleach or alcohol.
Anoxomat jars are designed specifically for use with the
Advanced Anoxomat System
2.4 Expected jar service life
The expected service life of Anoxomat Jars depends
on proper usage, frequency of processing, appropriate
cleaning/maintenance, and prevention of accidental
abuse. Under normal conditions, a jar processed twice
per week will have an expected life of about five years or
approximately 500 cycles.
It is important that jars are inspected frequently (about
once per month) for signs of excessive wear or damage.
If a jar appears to be visibly worn or damaged, then it
must be replaced. Also, closely inspect the jar if it fails
the Jar Test or Seal-Leak Test during processing and/or if
the Pump Time Exceeded or Fill Time Exceeded message
appears.
Deep, horizontal cracks, especially near the top, are
indications that the jar has been dropped or the clamps
have been tightened excessively. Fine, vertical cracks on
the surface of the jar may result from frequent use due
to flexing that occurs when the jar is evacuated and then
filled. It also may be indicative that the jar has been
cleaned with alcohol.
Immediately discontinue use of a jar if it shows
significant cracking or other signs of excessive wear.
Replace all jars with visible cracks where the clamp ring
is glued to the jar base, In addition, the jar base must be
replaced if it is cracked where the disc is glued to the
cylinder or if the disc separates from the cylinder.
Please use the image below as a guide for viewing of
cracks on the jars.
Crack in jar
2.5 Maintaining valves
NOTICE: Work with one valve at a time.
The valves are located on the rear panel of the chassis.
Periodically, they must be inspected to ensure that there
is no dust or debris obstructing the valve block and/or
the interior of the valves.
2.6 Cleaning valves
1. Unplug the Anoxomat.
2. Close o the gas pressure and disconnect all of the
gas hoses from the instrument.
3. Remove the two screws securing the top cover to
the chassis and tilt the cover forward.
4. Unscrew the thumb nut on top of the coil.