AFi ISA User manual

User Manual
│Revision 1.0 │
English version

ISA CENTRIFUGE
Models
AFI-CI060R
AFI-CI060R-E
AFI-CI060V
AFI-CI060V-E
Operation Manual
(120 VAC, 60 Hz and 230 VAC, 50/60 Hz)
Designed & Manufactured by:
AFI Centrifuge
Rue N. Copernic
ZI Nord Bazouges
53200 CHATEAU-GONTIER
FRANCE

602348
This document has been prepared with the utmost care. However, AFI
Centrifuge refuses to accept any responsibility in the event of errors or
omissions. The same applies to any damage resulting from the use of
information contained in this manual.
AFI Centrifuge
3, Rue Nicolas Copernic
ZA Nord Bazouges
53200 CHATEAU-GONTIER
FRANCE
[phone]

Original Instructions Rev.1.0 User Manual –Isa 1/ 42
You have just purchased one of the finest Laboratory Centrifuges available. With proper care, maintenance and
laboratory procedure, this centrifuge will provide you years of productive service. Please read this manual carefully to
familiarize you with proper installation, and operation of the centrifuge. Other information are available on our website,
www.aficentrifuge.com .
Warranty
AFI Centrifuge. warrants AFI-CI060 Series ISA Centrifuge that it will repair F.O.B. its factory or furnish without charge F.O.B.
its factory a similar part to replace any material in its equipment within 24 months after the date of sale if proved to the
satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall
be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories
are warranted only to the extent guaranteed by the original manufacturer, and this warranty shall not apply to any portion
of the equipment modified by the user. Claims under this warranty should be directed to AFI Centrifuge. setting forth in
detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment.
This warranty shall not apply to any AFI Centrifuge product or part thereof which has been subject to misuse, abuse,
accident, shipping damage, improper installation or service, or damage by fire, or flood. If the serial number of this product
is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety.
The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any
item, for any reason, contact AFI Centrifuge for a Return Authorization Number. This number must accompany all returns.
Any product shipped to AFI Centrifuge without this number will be returned refused shipment or collect freight.

Original Instructions Rev.1.0 User Manual –Isa 2/ 42
Explanation of Symbols
Safety alert symbol indicates a potentially hazardous situation
which, if not avoided, could result in death or serious injury
CAUTION used without the safety alert symbol indicates a
potentially hazardous situation which, if not avoided, may result
in property damage.
Safety alert symbol indicates a potentially hazardous situation
which, if not avoided, may result in minor or moderate injury.
Pinching: This warning symbol indicates the presence of a risk of
pinching when handling the lid.
Note:
Used for important information
Potential electrical hazard, only qualified person to access
Biohazard
Ground, Earth
Lead Free
Modification registration
Following modifications register updates of the manual.
Date
Revision
Modifications
Paragraph
Author
2018, october
1.0
Creation
All
F. LE CAM
CAUTION
!
CAUTION
WARNING
!


Original Instructions Rev.1.0 User Manual –Isa 3/ 42
Table of Contents
GENERAL INFORMATION ------------------------------------------------------------------------------------------------------ 5
DESCRIPTION.......................................................................................................................................................5
INTENDED USE .....................................................................................................................................................5
PROHIBITIONS: ....................................................................................................................................................5
SPECIFICATIONS AND ACCESSORIES ..........................................................................................................................6
1.4.1. SPECIFICATIONS ...................................................................................................................................................6
1.4.2. ACCESSORIES.......................................................................................................................................................7
SAFETY INSTRUCTIONS...........................................................................................................................................8
INFORMATION ON THE REFRIGERANT FLUID USED........................................................................................................8
INSTALLATION INSTRUCTIONS ------------------------------------------------------------------------------------------------ 9
SHIPMENT ..........................................................................................................................................................9
DAMAGED SHIPMENTS..........................................................................................................................................9
HANDLING AND TRANSPORT ...................................................................................................................................9
UNPACKING ........................................................................................................................................................9
PACKING LIST.......................................................................................................................................................9
MACHINE DESCRIPTION.......................................................................................................................................10
INTERFACE DESCRIPTION ......................................................................................................................................10
SITE PREPARATION..............................................................................................................................................10
ELECTRICAL &ENVIRONMENTAL REQUIREMENTS .....................................................................................................11
2.9.1. ELECTRICAL ENVIRONMENT ..................................................................................................................................11
2.9.2. ENVIRONMENTAL REQUIREMENTS .........................................................................................................................11
OPERATING THE ISA CENTRIFUGE -AFI-CI060--------------------------------------------------------------------------- 12
FIRST START ......................................................................................................................................................12
ROTOR ASSEMBLY...............................................................................................................................................12
POSITION THE BUCKETS .......................................................................................................................................13
FIRST POWERING................................................................................................................................................13
OPENING THE LID ...............................................................................................................................................13
ROTOR LOADING AND BALANCING .........................................................................................................................14
QUICK START-UP ................................................................................................................................................15
OPERATING ------------------------------------------------------------------------------------------------------------------ 16
SETTINGS..........................................................................................................................................................16
SET SPEED ........................................................................................................................................................16
SET DURATION...................................................................................................................................................16
SET TEMPERATURE (REFRIGERATING MODEL) ..........................................................................................................16
SET ACCELERATION SLOPE....................................................................................................................................17
SET BRAKING SLOPE ...........................................................................................................................................17
USE A PROGRAM TO CENTRIFUGE ..........................................................................................................................18
INTERRUPT CENTRIFUGATION................................................................................................................................18
OPEN AFTER AN ELECTRIC POWER FAILURE /EMERGENCY STOP ...................................................................................18
PRECOOLING .....................................................................................................................................................19
SETTING MODE .................................................................................................................................................19
4.11.1. SET SPEED UNIT /CENTRIFUGE FORCE ...................................................................................................................19
4.11.2. SET CENTRIFUGATION RADIUS ..............................................................................................................................20
4.11.3. SET TIME UNIT ..................................................................................................................................................20
4.11.4. SET TEMPERATURE UNIT (REFRIGERATING MODEL)...................................................................................................21
ADVANCED PARAMETERS .....................................................................................................................................21
SLEEP MODE......................................................................................................................................................22
CARE AND MAINTENANCE -------------------------------------------------------------------------------------------------- 23
CARE AND CLEANING ..........................................................................................................................................23
5.1.1. AUTHORIZED MAINTENANCE PRODUCTS..................................................................................................................23
5.1.2. CENTRIFUGE......................................................................................................................................................23
5.1.3. ROTOR &ACCESSORIES .......................................................................................................................................24
5.1.4. DISINFECTION /DECONTAMINATION......................................................................................................................24
DISMANTLING THE ROTOR....................................................................................................................................25
LIMITING THE DURATION OF OPERATION .................................................................................................................25
CHECKING AND TESTING ACCESSORIES ....................................................................................................................25
STORAGE OF ACCESSORIES....................................................................................................................................25

Original Instructions Rev.1.0 User Manual –Isa 4/ 42
ANNUAL MAINTENANCE.......................................................................................................................................25
ERROR MESSAGES,TROUBLESHOOTING,OPTION-DIAGNOSTICS..................................................................................26
REMINDER MESSAGES .........................................................................................................................................27
BACK TO MANUFACTURER....................................................................................................................................28
DISPOSAL AND RECYCLE----------------------------------------------------------------------------------------------------- 29
THEORETICAL BASICS OF CENTRIFUGATION ------------------------------------------------------------------------------- 30
BASICS .............................................................................................................................................................30
NOMOGRAM.....................................................................................................................................................31
LOGBOOK .........................................................................................................................................................31
RETURN COUPON ...............................................................................................................................................32
ANNEX 1: ACCESSORY SPECIFICATIONS------------------------------------------------------------------------------------ 33
AFI-RXN150 SWING-OUT ROTOR ........................................................................................................................33
NU-RHDWN-150 SWING-OUT ROTOR ................................................................................................................38
NU-FAN6-50 ANGLE ROTOR ..............................................................................................................................39
EC CONFORMITY DECLARATION-------------------------------------------------------------------------------------------- 41

Original Instructions Rev.1.0 User Manual –Isa 5/ 42
General Information
Description
The ISA Centrifuge is a laboratory benchtop serie of centrifuges that maximizes productivity while minimizing laboratory
required bench space. The centrifuge functionality allows the separation of substances of different densities held in
suspension or emulsion in a liquid using centrifugal force.
The ISA Centrifuge is available in two models, one ventilated and the other refrigerated.
The ISA Centrifuge ventilated model has a unique ventilation system to maximize performance within laboratory ambient
temperature conditions.
The ISA Centrifuge refrigerated model adds precise temperature control to further maximize performance.
The refrigeration system refrigerant used is CFC-free to meet regulations of refrigerant use.
ISA Ventilated Centrifuge
ISA Refrigerated Centrifuge
Intended use
The centrifuge is designed and manufactured for use with rotating accessories supplied by AFI Centrifuge. It is suitable for
separating substances of different densities held in suspension, or emulsion in a liquid.
The maximum density of substances should be 1.2 g/cm3.
The centrifuge must be used in an optimal state of operation and maintenance. The non-observance of the rule of use may
threaten the health of users or third persons, but it may also cause damages to the unit and/or surrounding equipment.
Users of the equipment must be trained on good centrifugation practices, as well specific usage of the ISA Centrifuge
models and Accessories. Any use outside the framework defined above is considered non-compliant.
AFI Centrifuge assumes no responsibility for injury due to improper use.
Prohibitions:
This centrifuge is not designed for the following uses and environments.
The following items are prohibited:
Flammable, explosive, toxic and radioactive materials
Materials which may react, causing a hazard
Contaminated materials not contained in an aerosol-barrier container
Rotating rotors and accessories that have exceeded their maximum use.
Rotating rotors and accessories that are not maintained according to the instructions in this manual,
or showing signs of wear and/or corrosion.
Rotating rotors and accessories not supplied by AFI Centrifuge.
Explosive environments
Radioactive environments
Only operate a centrifuge after taking all necessary safety measures.
Rotors and buckets should be removed from use in case of mechanical fault, trace, or
corrosion. These elements have a life cycle duration, engraved on their visible part:
To maintain safe conditions, it is imperative to replace them when the recommended duration is reached.
CAUTION
!
CAUTION
!

Original Instructions Rev.1.0 User Manual –Isa 6/ 42
Specifications and Accessories
Specifications
Model
Ventilated
Refrigerating
Benchtop centrifuge
Reference
AFI-CI060V-E
AFI-CI060V
AFI-CI060R-E
AFI-CI060R
Electrical specifications
Nominal voltage (+/- 10%)
230 V
120 V
230 V
120 V
Frequency
50 / 60 Hz
60 Hz
50 Hz
60 Hz
Max. Current
2A
4A
5A
10A
Current phase
1
Max. Power
300 W
800 W
Dimensions & Weight
Dimensions (HxWxD)
34 x 38.9 x 50.8 cm
13.4 x 15 x 19.7 in
34.1 x 45.9 x 70.2 cm
13.4 x 17.7 x 27.6 in
Dimensions on benchtop (WxD)
36.6 x 45.1 cm
14.2 x 17.7 in
44.1 x 64.5 cm
17.3 x 25.2 in
Package dimension (HxWxD)
49 x 57 x 66 cm
19.3 x 22.4 x 26.0 in
50 x 65 x 93 cm
19.7 x 25.6 x 36.6 in
Opened lid height
74 cm
29.1 in
80 cm
31.5 in
Weight unpacked
40 kg
70 kg
Weight packed
50 kg
80 kg
Centrifuge description
4.7’’ hight contrast screen

Knob + Tactile Touch

Microprocessor control

Automatic rotor recognition

Imbalance detection
Working area auto-calibration - Continuous vibration measurement
Cycle duration
10 s to 99h : 59 min : 59 s / Continuous cycle / Pulse
Temperature units
°C / °F
Centrifugation chamber
Inox
Max. density
1.2 g / cm3
Brushless induction motor

Speed accuracy
± 0.5%
Programs number
99
99+1
Pulse (short run)

Acceleration & Braking slopes
10 & 10
Brakeless mode

Cooling system
Ventilation device
Without CFC
Range of setting temperature
N/A
-10°C +40°C
Precool function
N/A

Temperature accuracy
N/A
+/- 2°C
Heat dissipation
1364 BTU/h
2729 BTU/h
Max. noise
≤ 58 dB(A)
≤ 54 dB(A)
Refrigerant fluid / Global Warming Potential (GWP)
N/A
R452A / 2141

Original Instructions Rev.1.0 User Manual –Isa 7/ 42
References
Description
Capacity
Lot
Swing-out Rotors, Buckets & Lids
AFI-RXN150
Swing-Out rotor 4x 150 ml
4x 150 ml
One unit
AFI-CPI150
Clinical Pack
36 x 5 ml
One unit
AFI-B150
150 ml Round Buckets
24 x 5 ml
Set of 4
AFI-SB150
150 ml Square Buckets
36 x 5 ml
Set of 4
AFI-DB50
Dedicated Buckets for 50/10 ml Conical
Tube
8 x 50 ml
Set of 4
AFI-L150
Biosafety secured lid for AFI-B150
-
Set of 4
AFI-LSB150
Biosafety secured lid for AFI-SB150
-
Set of 4
NU-RHDW-N150
Swinging Bucket Rotor
2 microplate carriers
One unit
NU-BHDW-N150
Microplate Carrier
2 x 3 Microplates
2 x 1 Deep-Weel
Set of 2
Adapters for Square buckets
-
AFI-06T50C-SB
50 ml Conical Tube
4 x 50 ml
AFI-06T50AOR-SB
50ml AOR* Round Tube
4 x 50 ml
AFI-06T50S-SB
50 ml Skirted Conical Tube
4 x 50 ml
AFI-06T30-SB
30 ml Conical Tube
4 x 30 ml
AFI-06T30S-SB
30 ml Skirted Conical Tube
4 x 30 ml
AFI-06T15C-SB
15ml Conical Tube
8 x 15 ml
AFI-06T10C-SB
10ml Conical Tube
16 x 10 ml
AFI-06T10-SB
10 ml Blood Collection Tube
14ml Urine Tube
16 x 10 ml / 14 ml
AFI-06T5-SB
5-7ml Blood Collection Tube
36 x 5 ml
AFI-06T2-SB
1,5 - 2ml MicroTube
72 x 2 ml
Adapters for Round buckets
(possibility set of 2)
-
Set of 4
AFI-06T80AOR
80ml AOR* Round Tube
4 x 80 ml
AFI-06T50C
50 ml Conical Tube
4 x 50 ml
AFI-06T50AOR
50ml AOR* Round Tube
4 x 50 ml
AFI-06T50S
50 ml Skirted Conical Tube
4 x 50 ml
AFI-06T30
30 ml Conical Tube
4 x 30 ml
AFI-06T30S
30 ml Skirted Conical Tube
4 x 30 ml
AFI-06T15C
15ml Conical Tube
8 x 15 ml
AFI-06T10C
10ml Conical Tube
12 x 10 ml
AFI-06T10
14ml Urine Tube
16 x 14 ml
AFI-06T10
10ml Blood Collection Tube
16 x 10 ml
AFI-06T5
5-7ml Blood Collection Tube
24 x 5 ml
AFI-06T2
1,5 - 2ml MicroTube
48 x 2 ml
Fixed Angled Rotor
NU-FAN6-50
6 x 50 ml Angular Rotor
6 x 50 ml
One unit
Adapters for Fixed Angled Rotor
AFI-50R50-OAK
50ml AOR* Round Tube
6 x 50 ml
AFI-50R30-C
30 ml Conical Tube
AFI-50R30-OAK
30ml AOR* Round Tube
6 x 30 ml
AFI-50R15-C
30ml AOR* Round Tube
6 x 30 ml
AFI-50R10-OAK
10ml AOR* Round Tube
6 x 10 ml
AFI-50R10
10ml Blood Collection Tube
Accessories Kits
71199003
Cleaner / Disinfectant
71199012
Tube of Grease 20 gr
71199020
Replacement Kit AFI-LS150 O-rings for lids
Set of 4

Original Instructions Rev.1.0 User Manual –Isa 8/ 42
Safety Instructions
The operator must observe the following precautions when using the
centrifuge:
Ensure stability and perfect levelling of the centrifuge.
Check the correct mounting of the rotor and its accessories before starting a centrifugation cycle.
Check tube resistance at maximum applied centrifugal force: chemical resistance to centrifuged products and
mechanical strength at the centrifugal force applied thereto.
Check the condition of the tubes and remove damaged tubes.
Use only the rotors and accessories authorized by the manufacturer AFI Centrifuge.
Swing-out rotors: install four (or two) buckets; never perform any cycle with missing buckets.
Maintain and control accessories.
Balance the load of the rotor around the axis of rotation.
Respect the maximum density of 1.2 g / cm3,in particular in case of a cycle at full speed.
Limit volume in case of excess density.
Install the centrifuge in a ventilated area, on a horizontal rigid support to absorb the vibrations generated by the
centrifuge.
During operation, the centrifuge must not be moved or be subject to impact.
Do not attempt to open the lid while the rotor is spinning.
Do not attempt to neutralize the motorized lid lock
Do not lean over the centrifuge during the spin cycle.
Do not stay within a space of 10 inch around the centrifuge longer than necessary.
Do not leave potentially dangerous materials inside the free space.
Use aerosol-barrier accessories when centrifuging bio hazardous material.
Condensation may form inside the centrifuge when it is moved from a cold environment to a warm environment.
Allow the centrifuge to warm up for two hours before use.
Information on the refrigerant fluid used
The "refrigerant" type apparatus contains fluorinated greenhouse gases under the Kyoto Protocol. They should therefore
not be released into the atmosphere. Repairs or disassembly of this appliance should only be carried out by qualified
personnel.
The data corresponding to the type of refrigerant, the Global Warming Potential (GWP) value, and the load are detailed in
§ 1.4.1.Specifications.
CAUTION
!

Original Instructions Rev.1.0 User Manual –Isa 9/ 42
Installation Instructions
Shipment
AFI Centrifuge takes every reasonable precaution to assure that your ISA Centrifuge arrives without damage. Motor carriers
are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can
occur in any shipment and the following outlines the steps you should take on receipt of a ISA Centrifuge to be sure that if
damage has occurred, the proper claims and actions are taken immediately.
Damaged Shipments
Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of the
freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis of your
filing the claim against the carrier.
If concealed damage is found it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE and request an inspection.
Without this inspection, the transportation company may not accept a claim for loss or damage. If the carrier will not
perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date and that he failed to
comply with the request. This along with other papers in the customer's possession will support the claim.
Handling and transport
The package containing the centrifuge needs to be handled by suitable means (pallet truck, etc.).
Please ensure that the people in charge of handling are qualified to handle the lifting equipment.
Maintain the following conditions during transport:
Temperature: -20 to 50 ° C
Relative humidity: <90%, under the dew point
Unpacking
Remove the cardboard packaging made of 2 parts (a lid and a bottom).
The ISA Centrifuge was packed with great care to prevent transport hazards. Before disposing of the packaging of the
centrifuge, and its spinning accessories, please make sure you are in possession of the following:
Packing list
ISA CENTRIFUGE
Compliance Certificate
User Manual
Power Cord
Rotor Fixing Wrench
Manual release key
Food grade grease
CAUTION
!

Original Instructions Rev.1.0 User Manual –Isa 10/ 42
Machine Description
Fig. 1 ¾ Front View
1. Lid
2. Window
3. Control panel
4. Opening lid button
5. Cooling unit
6. Electrical outlet
Interface description
1. Power ON-OFF, START-STOP, & cycle interruption button.
Can be activated through single press or press & hold action.
2. Display screen.
3. Validation button: Can be activated through single press or
press & hold action.
4. Setting knob. It is a lighting knob, giving information
concerning the centrifuge status, corresponding to colors changes.
5. Program number / Error number
6. Service key logo
7. Speed parameters : unit (rpm or xg), radius (mm)
8. Time unit : Hour / Minute or Minute / Second
9. Temperature unit (Celsius or Fahrenheit degree)
10. Slope parameters : Acceleration or Braking slope
Site preparation
Prepare a stable, clean and levelled support (e.g. laboratory bench). This support must be able to support the weight of the
centrifuge and its use, and be firm enough so as not to generate or maintain
vibration. Such vibrations would degrade the results of the centrifugation, and
may result in an unbalanced error.
A clearance envelope of 30 cm (11.8 inch) around the centrifuge should be
arranged according to IEC 61010-2-020: No potentially hazardous material,
nobody or no object shall be deposited within the free space.
Fig. 2 Open space around the centrifuge
It is recommended that no less than two people are present to manually lift the
centrifuge onto the laboratory bench.
CAUTION
!
2
1
3
5
4
6
2
1
3
4
4
5
8
7
6
9
30 cm
30 cm
Front Access
30 cm

Original Instructions Rev.1.0 User Manual –Isa 11/ 42
Electrical & Environmental Requirements
The centrifuge requires must be 230V/50Hz or 115V/60Hz, single phase, (current rating varies per centrifuge model, refer
to § 1.4.1 Specifications) according to the manufacturer sheet on the back of the unit.
The centrifuge must be plugged into an outlet with protective earthing connection with the standard power cord.
The electrical outlet into which the centrifuge is connected should be readily accessible for maintenance purposes.
The power cord plug serves as the main disconnect device and should remain readily accessible. The electrical outlet should
be on its own branch circuit, protected with a circuit breaker at the distribution panel near the centrifuge.
The centrifuge is designed for use under the following ambient conditions (according to EN 61010-1)
• Indoor use
• Maximum altitude: 2000 m
• Ambient temperature range of 5 to 40 ° C
• Maximum relative humidity of 80% for temperatures up to 22 ° C,
• Supply voltage fluctuation <± 10% of rated voltage.
• Overvoltage on the Category II power supply network (IEC 60364-4-44 standard)
• Maximum degree of pollution of the room: 2.0
• Walls less than 1m away must be able to stop the centrifuge from moving in the event of a major accident.
Maximum performance is ensured in the following ambient temperature range: 15 ° C to 25 ° C.

Original Instructions Rev.1.0 User Manual –Isa 12/ 42
Operating the ISA centrifuge - AFI-CI060
First Start
The first start must fulfill the conditions of the IEC 61010-2-020 safety standard
HOW TO GREASE A ISA CENTRIFUGE
Rotor assembly
Clean and lubricate the motor shaft by depositing a thin film of the
food grade grease supplied.
Place the rotor above the drive shaft.
Slide the rotor on the drive shaft.
Use the provided wrench to tighten the rotor nut onto the motor
shaft.
Remove the wrench
Clean and lubricate the pins by depositing a thin film of the food
grease supplied.
+
Grab the synthetic grease container provided with your centrifuge.
Dispense a small amount of grease on each of the following locations:
01

Original Instructions Rev.1.0 User Manual –Isa 13/ 42
Position the buckets
Swing-out rotors are used with round and square buckets or
microplate buckets.
Place the buckets & lids in the corresponding numbered locations.
First powering
After transport, wait at least 2 hours before putting the appliance
into operation so that the refrigerant circuit is fully efficient. Connect
the power cord to the mains.
Press & maintain the START-STOP button onto the control panel,
until display powering.
An animation starts during the initialization phase.
The parameters of a program appear.
Note: To enter the standby mode, press and hold the START / STOP
key: Only the knob remains illuminated.
Opening the lid
On the first start, the lid may not open right away due to a long storage in a cold
environment.
In this case, warm-up the hinge spring by repeated manual shifting of the lid
downwards.
To open the lid, press the button on the right side.
During a first start, it is possible that the lid does not open frankly after prolonged storage. In this case, activate the spring
hinges by manually manipulating the cover from top to bottom.
To close the lid, lower the lid and press until click.
CAUTION

Original Instructions Rev.1.0 User Manual –Isa 14/ 42
Rotor loading and balancing
Position the 4 numbered buckets in their corresponding positions on the rotor. Each
bucket should pivot freely in its slot.
Place the inserts corresponding to the tubes to be centrifuged in the four buckets.
The buckets must be symmetrically distributed.
For better balance, each numbered bucket must be placed in the corresponding position
marked on the rotor.
Each position on the rotor must be equipped with its bucket.
Do not use a rotor without its complete set of four buckets.
Maximum loads according to the type of rotor are specified in the annex.
The centrifuge tolerates an imbalance of 8 grams.
If the imbalance is higher than the tolerated threshold, an imbalance detection system
stops the centrifuge automatically.
Key logo and error message "02" appear. The rotor stops in free wheel mode without
braking system.
Excessive imbalance is likely to damage the turning components and/or the centrifuge.
Follow loading instructions referring to corresponding rotor description in § 9 Annex 1:
Accessory Specifications.


Original Instructions Rev.1.0 User Manual –Isa 15/ 42
Quick start-up
Before using the centrifuge, make sure it has been properly installed.

Original Instructions Rev.1.0 User Manual –Isa 16/ 42
Operating
Settings
Program setting is done adjusting successively following parameters:
Speed
Cycle Duration
Temperature
Acceleration Slope
Braking slope
Set Speed
#
Action
Instruction
Effect
1
Select the program number spinning
the knob.
The program number is displayed under
"Prog".
2
Press & hold validation button.
The selected program is now in
Modification mode.
Speed value is now blinking.
3
Modify blinking value spinning the
knob.
The speed value is modified.
Default setting unit is rotation per
minute (rpm).
To modify the unit, refer to § 4.11.1 Set
Speed Unit / Centrifuge Force
4
x6 (on
refrigerating
model)
Press validation button 6 x to validate.
(On ventilated model, press 5x)
The modification of the selected
program is saved.
Set duration
#
Action
Instruction
Effect
1
Select the program number spinning
the knob.
The program number is displayed under
"Prog".
2
Press & hold validation button.
The selected program is now in
Modification mode.
Speed value is now blinking.
3
x1
Press 1x to access to duration
parameter.
Duration value is blinking.
4
Modify blinking value spinning the
knob.
The duration value is modified.
Default setting unit is minutes : seconds
(min:sec).
5
x5 (on
refrigerating
model)
Press 5 x to validate.
(On ventilated model, press 4x)
The modification of the selected
program is saved.
Set Temperature (Refrigerating model)
#
Action
Instruction
Effect
1
Select the program number spinning
the knob.
The program number is displayed under
"Prog".
2
Press & hold validation button.
The selected program is now in
Modification mode.
Speed value is now blinking.
3
x2
Press 2x to access to temperature
parameter.
Duration value is blinking.