AFi LOREENA User manual

User Manual - Revision 1.3 - English Version

601997
This document has been prepared with the utmost care. However, AFI
Centrifuge refuses to accept any responsibility in the event of errors or
omissions. The same applies to any damage resulting from the use of
information contained in this manual.
AFI Centrifuge
3, Rue Nicolas Copernic
ZA Nord Bazouges
53200 CHATEAU-GONTIER
FRANCE
[phone]

Originale Instructions- Rev.1.3 User Maunal–Loreena 1/ 68
LOREENA CENTRIFUGE
Models
AFI-C300V
AFI –C300R
AFI-C300RF
AFI –C300V-E
AFI –C300R-E
AFI-C300RF-E
Operation Manual
(115 VAC, 60 Hz) (230 VAC, 50 Hz)
Manufactured by:
AFI Centrifuge
Rue N. Copernic
ZI Nord Bazouges
53200 CHATEAU-GONTIER
FRANCE

Originale Instructions- Rev.1.3 User Maunal–Loreena 2/ 68
Revision modifications
Date
Revision
Modifications
Paragraph
Author
August, 2018
1.3
End of cycle acquitment, Precool & Warmup
new shortcuts, "routine" program, End of
cycle Flashing signal, information menu
modifications; Add new rotors & rotor
specifications modifications.
4.6, 4.7, 4.9, 4.10,
4.11, 0, 4.14, 9
F. LE CAM

Originale Instructions- Rev.1.3 User Maunal–Loreena 3/ 68
You have just purchased one of the finest Laboratory Centrifuges available. With proper care, maintenance and
laboratory procedure, this centrifuge will provide you years of productive service. Please read this manual carefully to
familiarize you with proper installation, and operation of the centrifuge. Other information are available on our website,
www.aficentrifuge.com .
Warranty
AFI Centrifuge. warrants AFI-C200 Series LISA Centrifuge that it will repair F.O.B. its factory or furnish without charge F.O.B.
its factory a similar part to replace any material in its equipment within 24 months after the date of sale if proved to the
satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall
be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories
are warranted only to the extent guaranteed by the original manufacturer, and this warranty shall not apply to any portion
of the equipment modified by the user. Claims under this warranty should be directed to AFI Centrifuge. setting forth in
detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment.
This warranty shall not apply to any AFI Centrifuge product or part thereof which has been subject to misuse, abuse,
accident, shipping damage, improper installation or service, or damage by fire, or flood. If the serial number of this product
is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety.
The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any
item, for any reason, contact AFI Centrifuge for a Return Authorization Number. This number must accompany all returns.
Any product shipped to AFI Centrifuge without this number will be returned refused shipment or collect freight.
Explanation of Symbols
Safety alert symbol indicates a potentially hazardous situation
which, if not avoided, could result in death or serious injury
CAUTION used without the safety alert symbol indicates a
potentially hazardous situation which, if not avoided, may
result in property damage.
Safety alert symbol indicates a potentially hazardous situation
which, if not avoided, may result in minor or moderate injury.
Pinching: This warning symbol indicates the presence of a risk
of pinching when handling the lid.
Note:
Used for important information
Potential electrical hazard, only qualified person to access
Biohazard
Ground, Earth
Lead Free
WARNING
CAUTION
CAUTION


Originale Instructions- Rev.1.3 User Maunal–Loreena 4/ 68
Table of Contents
1. GENERAL INFORMATION ------------------------------------------------------------------------------------------------------ 6
1.1. DESCRIPTION.......................................................................................................................................................6
1.2. INTENDED USE .....................................................................................................................................................6
1.3. PROHIBITIONS: ....................................................................................................................................................7
1.4. MODELS,SPECIFICATIONS AND ACCESSORIES.............................................................................................................8
1.4.1. MODELS &SPECIFICATIONS ...................................................................................................................................8
1.4.2. ACCESSORIES.......................................................................................................................................................9
1.5. SAFETY INSTRUCTIONS.........................................................................................................................................12
1.6. INFORMATION ON THE REFRIGERANT FLUID USED......................................................................................................12
2. INSTALLATION INSTRUCTIONS ---------------------------------------------------------------------------------------------- 13
2.1. SHIPMENT ........................................................................................................................................................13
2.1.1. DAMAGED SHIPMENTS........................................................................................................................................13
2.1.2. HANDLING AND TRANSPORT .................................................................................................................................13
2.2. SITE PREPARATION..............................................................................................................................................13
2.3. ELECTRICAL ENVIRONMENT ..................................................................................................................................14
2.4. UNPACKING ......................................................................................................................................................14
2.5. PACKING LIST.....................................................................................................................................................14
2.6. DESCRIPTION.....................................................................................................................................................15
3. OPERATING THE LOREENA CENTRIFUGE -AFI-C300 ------------------------------------------------------------------- 16
3.1. FIRST START /POWERING....................................................................................................................................16
3.2. LEVELLING (FLOOR MODEL)..................................................................................................................................17
3.3. ROTOR ASSEMBLY...............................................................................................................................................17
3.4. POSITION THE BUCKETS .......................................................................................................................................17
3.5. FIRST POWERING................................................................................................................................................17
3.6. USING THE INTERFACE .........................................................................................................................................18
3.7. OPENING THE LID ...............................................................................................................................................18
3.8. ROTOR LOADING AND BALANCING .........................................................................................................................18
3.9. QUICK START-UP ................................................................................................................................................19
4. OPERATING ------------------------------------------------------------------------------------------------------------------ 20
4.1. SETTINGS..........................................................................................................................................................20
4.2. CREATE /EDIT A PROGRAM NAME .........................................................................................................................20
4.3. SET PROGRAM SETTINGS ......................................................................................................................................21
4.4. SET SPEED IN RPM OR RCF .................................................................................................................................22
4.5. PROTECT THE SETTINGS OF A PROGRAM ..................................................................................................................23
4.6. CENTRIFUGE:USE A PROGRAM .............................................................................................................................23
4.7. INTERRUPT CENTRIFUGATION................................................................................................................................24
4.8. OPEN AFTER AN ELECTRIC POWER FAILURE /EMERGENCY STOP ...................................................................................24
4.9. PRECOOLING .....................................................................................................................................................25
4.10. PREWARMING ...................................................................................................................................................25
4.11. ROUTINE MENU.................................................................................................................................................26
4.12. OTHER MENUS...................................................................................................................................................26
4.12.1. USER PREFERENCES ............................................................................................................................................28
4.12.2. TYPE OF SPEED DISPLAYED (RPM OR RCF) .............................................................................................................29
4.12.3. FREQUENCY OF LUBRICATION................................................................................................................................30
4.12.4. ACCESS TO LOGBOOK ..........................................................................................................................................30
4.12.5. ACCESS TO ERROR LOGBOOK.................................................................................................................................30
4.12.6. TYPE OF TEMPERATURE DISPLAYED ........................................................................................................................31
4.12.7. TIMER MODE.....................................................................................................................................................31
4.12.8. COUNTING MODE...............................................................................................................................................32
4.12.9. KEYBOARD TYPE .................................................................................................................................................32
4.12.10. CHANGE BACKGROUND COLOR..............................................................................................................................33
4.12.11. END OF CYCLE ALARM.........................................................................................................................................33
4.12.12. ERROR MESSAGE ALARM.....................................................................................................................................33
4.12.13. AUTOMATIC LID OPENING ....................................................................................................................................33
4.12.14. SLEEP MODE......................................................................................................................................................34
4.12.15. POST COOL .......................................................................................................................................................34
4.12.16. PRE COOL FUNCTION ..........................................................................................................................................35
4.12.17. TEMPERATURE DISPLAY MODE .............................................................................................................................35

Originale Instructions- Rev.1.3 User Maunal–Loreena 5/ 68
4.13. SERVICE MENU..................................................................................................................................................36
4.14. INFORMATION ...................................................................................................................................................36
4.15. ENERGY SAVING .................................................................................................................................................36
5. CARE AND MAINTENANCE -------------------------------------------------------------------------------------------------- 37
5.1. CARE AND CLEANING ..........................................................................................................................................37
5.1.1. AUTHORIZED MAINTENANCE PRODUCTS..................................................................................................................37
5.1.2. CENTRIFUGE......................................................................................................................................................37
5.1.3. ROTOR &ACCESSORIES .......................................................................................................................................38
5.1.4. DISINFECTION /DECONTAMINATION......................................................................................................................38
5.2. DISMANTLING THE ROTOR....................................................................................................................................39
5.3. LIMITING THE DURATION OF OPERATION .................................................................................................................39
5.4. CHECKING AND TESTING ACCESSORIES ....................................................................................................................40
5.5. STORAGE OF ACCESSORIES....................................................................................................................................40
5.6. ANNUAL MAINTENANCE.......................................................................................................................................40
5.7. UNBALANCING SENSITIVITY DETECTION ...................................................................................................................41
5.8. ERROR MESSAGES,TROUBLESHOOTING,OPTION-DIAGNOSTICS..................................................................................42
5.9. WEARING PARTS &CONSUMMABLE.......................................................................................................................45
5.10. BACK TO MANUFACTURER....................................................................................................................................45
6. ELECTRICAL/ENVIRONMENTAL REQUIREMENTS -------------------------------------------------------------------------- 46
6.1. ELECTRICAL (SUPPLY VOLTAGE FLUCTUATIONS NOT TO EXCEED +/-10%) ....................................................................46
6.2. OPERATIONAL PERFORMANCE (FOR INDOOR USE ONLY).............................................................................................46
6.3. LIGHT EXPOSURE................................................................................................................................................46
6.4. INSTALLATION CATEGORY:2.0..............................................................................................................................46
6.5. POLLUTION DEGREE:2.0 ....................................................................................................................................46
6.6. CHEMICAL EXPOSURE..........................................................................................................................................46
6.7. EMC PERFORMANCE (CLASSIFIED FOR LIGHT INDUSTRIAL)..........................................................................................46
7. DISPOSAL AND RECYCLE----------------------------------------------------------------------------------------------------- 47
8. THEORETICAL BASICS OF CENTRIFUGATION ------------------------------------------------------------------------------- 48
8.1. BASICS .............................................................................................................................................................48
8.2. NOMOGRAM.....................................................................................................................................................49
8.3. LOGBOOK .........................................................................................................................................................50
8.4. RETURN COUPON ...............................................................................................................................................51
9. ANNEX 1: ACCESSORY SPECIFICATIONS------------------------------------------------------------------------------------ 52
9.1. SWING-OUT ROTOR NU-RX1000 ........................................................................................................................52
9.2. SWING-OUT ROTOR NU-RX750 ..........................................................................................................................55
9.3. SWING-OUT ROTOR NU-RHDW..........................................................................................................................58
9.4. ANGULAR ROTOR AFI-RHD3...............................................................................................................................59
9.5. ANGLE ROTOR NU-RA8-50 ................................................................................................................................62
9.6. ANGLE ROTOR NU-RA30-15 ..............................................................................................................................63
9.7. ANGLE ROTOR NU-RA16-5 ................................................................................................................................64
9.8. ANGLE ROTOR NU-RA48-2 ................................................................................................................................65
9.9. ANGLE ROTOR NU-RA30-2 ................................................................................................................................66
10. EC CONFORMITY DECLARATION-------------------------------------------------------------------------------------------- 67

Originale Instructions- Rev.1.3 User Maunal–Loreena 6/ 68
LOREENA CENTRIFUGE
1. General Information
1.1.Description
The LOREENA Centrifuge is a laboratory series of centrifuges that maximizes productivity while minimizing laboratory
required bench space. The centrifuge functionality allows the separation of substances of different densities held in
suspension or emulsion in a liquid using centrifugal force.
The LOREENA Centrifuge is available in two models, one ventilated and the other Refrigerating.
The LOREENA Centrifuge ventilated model has a unique ventilation system to maximize performance within laboratory
ambient temperature conditions.
The LOREENA Centrifuge Refrigerating model adds precise temperature control to further maximize performance.
The refrigeration system refrigerant used is CFC-free to meet regulations of refrigerant use.
Benchtop ventilated model
Benchtop Refrigerating model
Floor Refrigerating Model
LOREENA Centrifuges
1.2.Intended use
The centrifuge is designed and manufactured for use with rotating accessories supplied by AFI Centrifuge. It is suitable for
separating substances of different densities held in suspension, or emulsion in a liquid.
The maximum density of substances should be 1.2 g/ml.
The centrifuge must be used in an optimal state of operation and maintenance. The non-observance of the rule of use may
threaten the health of users or third persons, but it may also cause damages to the unit and/or surrounding equipment.
Users of the equipment must be trained on good centrifugation practices, as well specific usage of the LOREENA
CENTRIFUGE models and Accessories. Any use outside the framework defined above is considered non-compliant.
AFI Centrifuge. assumes no responsibility for injury due to improper use.
CAUTION

Originale Instructions- Rev.1.3 User Maunal–Loreena 7/ 68
1.3.Prohibitions:
The centrifuge is not designed for the following uses and environments.
The following are prohibited:
Flammable, explosive, toxic and radioactive materials
Materials which may react, causing a hazard
Contaminated materials not contained in an aerosol-barrier container
Rotating rotors and accessories that have exceeded their maximum use.
Rotating rotors and accessories that are not maintained according to the instructions in this manual,
or showing signs of wear and/or corrosion.
Rotating rotors and accessories not supplied by AFI Centrifuge.
Explosive environments
Radioactive environments
Only operate a centrifuge after taking all necessary safety measures.
Rotors and buckets should be removed from use in case of mechanical fault,
trace, or corrosion. These elements have a life cycle duration, engraved on
their visible part: To maintain safe conditions, it is imperative to replace them
when the recommended duration is reached.
CAUTION

Originale Instructions- Rev.1.3 User Maunal–Loreena 8/ 68
1.4.Models, Specifications and Accessories
1.4.1. Models & Specifications
Description
Laboratory Centrifuge
Environmental Conditions
For indoor use only
Environment Temperature Range: : 60⁰F - 85⁰F (15⁰C - 30⁰C)
Environment Humidity: Maximum relative humidity 80% for
temperatures up to 31⁰C decreasing linearly to 50% relative humidity at 40⁰C
Environment Altitude: 6562 Feet (2000 meters) above sea level
Maximum capacity
4x1000 ml x kg / bucket, 4 x buckets
Models
Benchtop
Ventilated
Benchtop
Refrigerating
Floor
Refrigerating
Catalogue Numbers
AFI-C300V
AFI-C300V-E
AFI-C300R
AFI-C300R-E
AFI-C300RF
AFI-C300RF-E
Supply Voltage
(Depending on the model)
115 V 
10% 60
Hz
230 V 10%
50 / 60 Hz
115 V 
10% 60
Hz
230 V 10%
50Hz
115 V 10%
60 Hz
230 V 10%
50Hz
Maximum Power Consumed
1000 Watt
1700 Watt
1700 Watt
Maximum Speed (rpm)
15,300 rpm
(with AFI-RA30-2 rotor)
16,000 rpm
(with AFI-RA30-2 rotor)
Maximum Relative Centrifugal
Force (g)
25,648 xg
(with AFI-RA30-2 rotor)
28, 048 xg
(with AFI-RA30-2 rotor)
Dissipated Heat (Btuh)
(1 Btuh = 0.29307 W)
3412 Btuh
5800 Btuh
Fluid Coolant / Global Warming
Potential (GWP)
Not applicable
R404A / 3922
Fluid Charge / Ton Equivalent
CO2
Not applicable
0.81 kg / 3.18 Teq CO2
Ambient Operating Temperature
20⁰C ± 2⁰C
20⁰C ± 2⁰C
Temperature adjustment range
N/A
-10 ° C to 40 ° C
Temperature control precision
N/A
± 2 ° C
Footprint Dimensions (HxWxD)
37 x 50 x 61 cm
35 x 77 x 63 cm
83 x 57 x 64 cm
Net weight (excluding rotor)
82 kg /181 lbs.
145 kg / 320 lbs.
170 kg / 375 lbs
Packing (H x W x D) / Weight
54 x 74 x 77 cm
97 kg / 214 lbs
54 x 90 x 80 cm
160 kg / 353 lbs
100 x 80 x 80 cm
185 kg / 408 lbs
Maximum sound level
61 dB(A)
60 dB(A)
57 dB(A)
Integrated systems
Imbalance detection sensor
Motorized lid lock
Rotor speed control
Control System
Microprocessor
Acceleration slopes
9
Braking slopes
10
Speed control precision
± 10 rpm
Number of programs
99
99 + Pre-cooling + Pre-warming

Originale Instructions- Rev.1.3 User Maunal–Loreena 9/ 68
1.4.2. Accessories
Catalog Number
Description
Rotor capacity
Lot
Rotor & accessories:
NU-RX1000
Swing-Out rotor 4x 1000 ml
/
One unit
NU-B1000
1000 ml buckets
/
Set of 4
NU-L1000
Lid 1000 ml
/
Set of 4
NU-BXDW4
Microplates carriers
/
Set of 4
Adapters :
AFI-4T1000F
1000 ml Flat Bottle
4 x 1000 ml
Set of 4
AFI-4T750F
750 ml Flat Bottle
4x 750 ml
Set of 4
AFI-4T625F
625 ml Flat Bottle
4 x 625 ml
Set of 4
AFI-4T500F
500 ml Flat Bottle
4 x 500 ml
Set of 4
AFI-4T500C
500 ml Conical Bottle
4x 500 ml
Set of 4
AFI-4T290F
290 ml Flat Bottle
8 x 290 ml
Set of 4
AFI-4T250F
250 ml Flat Bottle
8 x 250 ml
Set of 4
AFI-4T250C
250 ml Conical Bottle (Without Lid bucket)
8 x 250 ml
Set of 4
AFI-4T200F
200 ml Flat Bottle
8 x 200 ml
Set of 4
AFI-4T200
200 ml Conical Bottle
8 x 200 ml
Set of 4
AFI-4T200
150 ml Conical Bottle
8 x 150 ml
Set of 4
AFI-4T100-C
100 ml Conical Bottle
8 x 100 ml
Set of 4
AFI-4T50C
50 ml Conical Tube
40 x 50 ml
Set of 4
AFI-4T50AOR
50ml AOR Advanced Oak Ridge
40 x 50 ml
Set of 4
AFI-4T50S
50 ml Skirted Conical Tube
28 x 50 ml
Set of 4
AFI-4T30S
30ml Skirted Conical Tube
40 x 30 ml
Set of 4
AFI-4T15C
15ml Conical Tube
96 x 15 ml
Set of 4
AFI-4T10
14ml Urine Tube
156 x 14 ml
Set of 4
AFI-4T10
10ml Blood Collection Tube
156 x 10 ml
Set of 4
AFI-4T5
5-7ml Blood Collection Tube
200 x 5 ml
Set of 4
AFI-4T2
1,5 - 2ml MicroTube
200 x 2 ml
Set of 4
Set of 4
Rotor & accessories:
NU-RX750
Swing-Out rotor 4x 750 ml
/
One unit
NU-750
750 ml buckets
/
Set of 4
NU-L750
Lid 750 ml
/
Set of 4
NU-BXDW3
Microplate Bucket X4
Set of 4
Adapters :
AFI-3T750F
750 ml Flat Bottle
4 x 750 ml
Set of 4
AFI-3T625F
625 ml Flat Bottle (AFI)
4 x 525 ml
Set of 4
AFI-3T500F
500 ml Flat Bottle
4 x 500 ml
Set of 4
AFI-3T500C
500 ml Conical Bottle
4 x 500 ml
Set of 4
AFI-3T400F
400 ml Flat Bottle
4 x 400 ml
Set of 4
AFI-3T290F
290 ml Flat Bottle
4 x 290 ml
Set of 4
AFI-3T250F
250 ml Flat Bottle
4 x 250 ml
Set of 4
AFI-3T250C
250 ml Conical Bottle (Without Lid bucket)
4 x 250 ml
Set of 4
AFI-3T200F
200 ml Flat Bottle
4 x 200 ml
Set of 4
AFI-3T200C
200 ml Conical Bottle
4 x 200 ml
Set of 4
AFI-3T200C
150 ml Conical Bottle
4 x 150 ml
Set of 4
AFI-3T100C
100 ml Conical Bottle
4 x 100 ml
Set of 4
AFI-3T50CL
50 ml Conical Tube
24 x 50 ml
Set of 4
AFI-3T50C
50 ml Conical Tube (Without Lid bucket)
28 x 50 ml
Set of 4
AFI-3T50AOR
50ml AOR Advanced Oak Ridge
24 x 50 ml
Set of 4
AFI-3T50S
50 ml Skirted Conical Tube (Without Lid bucket)
28 x 50 ml
Set of 4
AFI-3T50SL
50 ml Skirted Conical Tube
24 x 50 ml
Set of 4
AFI-3T30S
30ml Skirted Conical Tube
28 x 30 ml
Set of 4

Originale Instructions- Rev.1.3 User Maunal–Loreena 10/ 68
AFI-3T15C
15ml Conical Tube
56 x 15 ml
Set of 4
AFI-3T10
14ml Urine Tube
84 x 14 ml
Set of 4
AFI-3T10
10ml Blood Collection Tube
84 x 14 ml
Set of 4
AFI-3T5
5-7ml Blood Collection Tube
108 x 5 ml
Set of 4
AFI-3T2
1,5 - 2ml MicroTube
216 x 2 ml
Set of 4
Rotor & accessories:
NU-RHDW
Microplate Rotor H
One unit
NU-BHDW
Microplate Bucket H
2 x 4 Microplates
2 x 1 Deep-Weel
Set of 2
NU-LH
Biosafety secured lid for BHDW set of 2
Set of 2
Maintenance :
71199012
Tube of grease 20gr
One unit
Angular Rotor
NU-RA6-100
6 x 100 ml
One unit
Adapters
AFI-100R80-OAK 6 x 80ml
80ml AOR Advanced Oak Ridge
6 x 80ml
Set of 6
AFI-100R50-C 6 x 50ml
50ml Conical Tube
6 x 50ml
Set of 6
AFI-100R50-OAK 6 x 50ml
50ml AOR Advanced Oak Ridge
6 x 50ml
Set of 6
AFI-100R30-OAK 6 x 30ml
30ml AOR Advanced Oak Ridge
6 x 30ml
Set of 6
AFI-100R15-C 6 x 15ml
15ml Conical Tube
6 x 15ml
Set of 6
AFI-100R10-OAK 12 x 10ml
10ml AOR Advanced Oak Ridge
12 x 10ml
Set of 6
AFI-100R10 12 x 10ml
10ml Blood Collection Tube
12 x 10ml
Set of 6
AFI-100R5 18 x 5/7ml
5-7ml Blood Collection Tube
18 x 5/7ml
Set of 6
AFI-100R5 12 x 5ml
5ml MicroTube
12 x 5ml
Set of 6
AFI-100R2 18 x 2ml
1,5 - 2ml MicroTube
18 x 2ml
Set of 6
Rotor
NU-RA8-50
Angular Rotor
8 x 50 ml
One unit
Adapters
AFI-50R50-R
50ml Round Tube
8 x 50 ml
Set of 8
AFI-50R50-OAK
50ml AOR Advanced Oak Ridge
8 x 50ml
Set of 8
AFI-50R30-OAK
30ml AOR Advanced Oak Ridge
8 x 30ml
Set of 8
AFI-50R15-C
15ml Conical Tube
8 x 15ml
Set of 8
AFI-50R10-OAK
10ml AOR Advanced Oak Ridge
8 x 10ml
Set of 8
AFI-50R10
10ml Blood Collection Tube
8 x 10ml
Set of 8

Originale Instructions- Rev.1.3 User Maunal–Loreena 11/ 68
Rotor
NU-RA30-15
Angular Rotor
30 x 15 ml
Adapters
AFI-15R15-C
15ml Conical Tube
30 x 15 ml
AFI-15R15-R
15ml Round Tube
30 x 15 ml
AFI-15R10-OAK
10ml AOR Advanced Oak Ridge
30 x 10 ml
AFI-15R10
10ml Blood Collection Tube
30 x 10 ml
AFI-15R5
5-7ml Blood Collection Tube
30 x 5 ml
Rotor
NU-RA16-5
Angular Rotor
16 x 5 ml
Rotor
NU-RA48-2
Angular Rotor
48 x 2 ml
Adapters
AFI-48R05
0,5ml MicroTube
48 x 2 ml
AFI-48R025
0,25ml MicroTube
48 x 2 ml
AFI-48R02
0,2ml MicroTube
48 x 2 ml
Rotor
NU-RA30-2
Angular Rotor
30 x 2 ml
Adapters
AFI-30R05
0,25ml MicroTube
30 x 2 ml
AFI-30R025
0,2ml MicroTube
30 x 2 ml
AFI-30R02
0,5ml MicroTube
30 x 2 ml

Originale Instructions- Rev.1.3 User Maunal–Loreena 12/ 68
1.5.Safety Instructions
The operator must observe the following precautions when using the centrifuge:
Ensure stability and perfect levelling of the centrifuge.
Check the correct mounting of the rotor and its accessories before starting a centrifugation cycle.
Check tube resistance at maximum applied centrifugal force: chemical resistance to centrifuged products and
mechanical strength at the centrifugal force applied thereto.
Check the condition of the tubes and remove damaged tubes.
Use only the rotors and accessories authorized by the manufacturer AFI Centrifuge.
Swing-out rotors: install four (or two) buckets; never perform any cycle with missing buckets.
Maintain and control accessories.
Balance the load of the rotor around the axis of rotation.
Respect the maximum density of 1.2 g / ml,in particular in case of a cycle at full speed.
Limit volume in case of excess density.
Install the centrifuge in a ventilated area, on a horizontal rigid support to absorb the vibrations generated by the
centrifuge.
During operation, the centrifuge must not be moved or be subject to impact.
Do not attempt to open the lid while the rotor is spinning.
Do not attempt to neutralize the motorized lid lock
Do not lean over the centrifuge during the spin cycle.
Do not stay within a space of 12 inch around the centrifuge longer than necessary.
Do not leave potentially dangerous materials inside the free space.
Use aerosol-barrier accessories when centrifuging bio hazardous material.
Condensation may form inside the centrifuge when it is moved from a cold environment to a warm environment.
Allow the centrifuge to warm up for two hours before use.
1.6.Information on the refrigerant fluid used
The "refrigerant" type apparatus contains fluorinated greenhouse gases under the Kyoto Protocol. They should therefore
not be released into the atmosphere. Repairs or disassembly of this appliance should only be carried out by qualified
personnel.
The data corresponding to the type of refrigerant, the Global Warming Potential (GWP) value, and the load are detailed in
§ 1.4.1.Models & Specifications.

Originale Instructions- Rev.1.3 User Maunal–Loreena 13/ 68
2. Installation Instructions
2.1.Shipment
AFI Centrifuge takes every reasonable precaution to assure that your LOREENA Centrifuge arrives without damage. Motor
carriers are carefully selected and shipping cartons have been specially designed to insure your purchase. However,
damage can occur in any shipment and the following outlines the steps you should take on receipt of a LOREENA Centrifuge
to be sure that if damage has occurred, the proper claims and actions are taken immediately.
2.1.1. Damaged Shipments
Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of the
freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis of your
filing the claim against the carrier.
If concealed damage is found it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE and request an inspection.
Without this inspection, the transportation company may not accept a claim for loss or damage. If the carrier will not
perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date and that he failed to
comply with the request. This along with other papers in the customer's possession will support the claim.
2.1.2. Handling and transport
The package containing the centrifuge needs to be handled by suitable means (pallet truck, etc.).
Please ensure that the people in charge of handling are qualified to handle the lifting equipment.
Maintain the following conditions during transport:
Temperature: -20 to 50 ° C
Relative humidity: <90%, under the dew point
2.2.Site preparation
Prepare a stable, clean and levelled support (e.g. laboratory bench). This support must be able to support the weight of the
centrifuge and its use, and be firm enough so as not to
generate or maintain vibration. Such vibrations would
degrade the results of the centrifugation, and may result in an
unbalanced error.
A clearance of 12 inch (300mm) around the centrifuge should
be arranged according to IEC 61010-2-020: No potentially
hazardous material, nobody or no object shall be deposited
within the free space.
Open space around the centrifuge
It is recommended that no less than two people are present
to manually lift the centrifuge onto the laboratory bench.
CAUTION
CAUTION
12”
12”
Access area
12”

Originale Instructions- Rev.1.3 User Maunal–Loreena 14/ 68
2.3.Electrical environment
The centrifuge requires must be 230V/50Hz or 115V/60Hz, single phase, (current rating varies per centrifuge model,
reference Electrical/Environmental Requirements).according to the manufacturer sheet on the back of the unit.
The centrifuge must be plugged into an outlet with protective earthing connection with the standard power cord.
The electrical outlet into which the centrifuge is connected should be readily accessible for maintenance purposes.
Do not position the centrifuge to prevent access to the power cord.
The power cord plug serves as the main disconnect device and should remain readily accessible. The electrical outlet should
be on its own branch circuit, protected with a circuit breaker at the distribution panel near the centrifuge.
2.4.Unpacking
Remove the cardboard packaging made of 2 parts (a lid and a bottom).
For floor model centrifuge, let a minimum of 1 meter space behind the wooden ramp lied on the floor before getting down
the device.
The LOREENA Centrifuge was packed with great care to prevent transport hazards. Before disposing of the packaging of
the centrifuge, and its spinning accessories, please make sure you are in possession of the following:
2.5.Packing list
1) 2)
or
3)
Or
LOREENA CENTRIFUGE Model
Compliance Certificate
QuickStart Guide
1)
2)
3)
Power Cord
Manual release key (shape
according centrifuge model)
Food grade grease
Centrifuge models :
1) Ventilated or
2) Refrigerating or
3) Floor refrigerating

Originale Instructions- Rev.1.3 User Maunal–Loreena 15/ 68
2.6.Description
Fig. 1 Refrigerating centrifuge
1) Lid
2) Window
3) Control panel
4) Electronic warhead
5) Main switch
6) Cooling unit (Refrigerating model only)
7) Electrical outlet
Fig. 2 Floor centrifuge
8) Wheels (x4)
9) Adjustable pads (x2)
2
1
3
5
4
6
7
1
6
4
5
9
8

Originale Instructions- Rev.1.3 User Maunal–Loreena 16/ 68
3. Operating the LOREENA centrifuge - AFI-C300
3.1.First Start / Powering
The first start must fulfill the conditions of the IEC 61010-2-020 safety standard
HOW TO GREASE A LOREENA CENTRIFUGE

Originale Instructions- Rev.1.3 User Maunal–Loreena 17/ 68
3.2.Levelling (Floor model)
Mandatory operation :
Before using floor model centrifuge, adjust the level setting pads,
following the procedure supplied onto the package box
(Document Reference AP3.001 Unpacking & installation).
Use supplied pads.
3.3.Rotor assembly
Clean and lubricate the motor shaft by depositing a thin film of
the food grade grease supplied.
Place the rotor above the drive shaft.
Slide the rotor on the drive shaft.
A click indicates that the rotor is locked.
Clean and lubricate the pins by depositing a thin film of the food
grease supplied.
3.4.Position the buckets
Swing-out rotors are used with round buckets or micro titration
buckets.
Place the buckets in the corresponding numbered locations.
3.5.First powering
Activate the switch on the bottom right of the control center.
On/Off Switch position
Under Control Center

Originale Instructions- Rev.1.3 User Maunal–Loreena 18/ 68
3.6.Using the interface
3.7.Opening the lid
On the first start, the lid may not open right away due to a long
storage in a cold environment.
In this case, warm-up the jacks by repeated manual shifting
of the lid downwards.
To open the lid, press the button on the right side.
3.8.Rotor loading and balancing
Position the 4 numbered buckets in their corresponding positions
on the rotor. Each bucket should pivot freely in its slot.
Place the inserts corresponding to the tubes to be centrifuged in
the four buckets.
The buckets must be symmetrically distributed.
For better balance, each numbered bucket must be placed in the
corresponding position marked on the rotor.
Each position on the rotor must be equipped with its bucket.
Do not use a rotor without its complete set of four buckets.
Maximum loads according to the type of rotor are specified in the
annex.
The centrifuge tolerates an imbalance of 15 grams.
If the imbalance is higher than the tolerated threshold, an
imbalance detection system stops the centrifuge automatically.
The following message appears: "Imbalance" and the rotor stops in
free wheel mode without braking system.
Excessive imbalance is likely to damage the turning components
and/or the centrifuge.
Loading the buckets
Allowed:
Forbidden :
Loading the rotor
Allowed:
Symmetrical loading
Forbidden:
Incomplete loading
Asymmetrical loading
1. Touch screen for direct access to various functions.
2. Single button for easy operation.
This button allows the validation, the start of a cycle, but also the
interruption of a cycle, and the output of a menu.
The functions accessible through this single button are activated
as required by the microcontroller.
2
1