AFi SIRENA AFI-C060R User manual

User Manual
│Revision 1.0 │
English version

SIRENA CENTRIFUGE
Models
AFI-C060R
AFI-C060R-E
User Manual
(230 VAC, 50 Hz, 120VAC, 60 Hz)
Designed & Manufactured by:
AFI Centrifuge
Rue N. Copernic
ZI Nord Bazouges
53200 CHATEAU-GONTIER
FRANCE

602363
This document has been prepared with the utmost care. However, AFI
Centrifuge refuses to accept any responsibility in the event of errors or
omissions. The same applies to any damage resulting from the use of
information contained in this manual.
AFI Centrifuge
3, Rue Nicolas Copernic
ZA Nord Bazouges
53200 CHATEAU-GONTIER
FRANCE
[phone]

Original Instructions Rev.1.0 User Manual –Sirena 1/ 56
You have just purchased one of the finest Laboratory Centrifuges available. With proper care, maintenance and
laboratory procedure, this centrifuge will provide you years of productive service. Please read this manual carefully to
familiarize you with proper installation, and operation of the centrifuge. Other information are available on our website,
www.aficentrifuge.com .
Warranty
AFI Centrifuge. warrants AFI-CI060 Series ISA Centrifuge that it will repair F.O.B. its factory or furnish without charge
F.O.B. its factory a similar part to replace any material in its equipment within 24 months after the date of sale if proved
to the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective
shall be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or
accessories are warranted only to the extent guaranteed by the original manufacturer, and this warranty shall not apply to
any portion of the equipment modified by the user. Claims under this warranty should be directed to AFI Centrifuge.
setting forth in detail the nature of the defect, the date of the initial installation and the serial and model number of the
equipment.
This warranty shall not apply to any AFI Centrifuge product or part thereof which has been subject to misuse, abuse,
accident, shipping damage, improper installation or service, or damage by fire, or flood. If the serial number of this
product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety.
The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any
item, for any reason, contact AFI Centrifuge for a Return Authorization Number. This number must accompany all
returns. Any product shipped to AFI Centrifuge without this number will be returned refused shipment or collect freight.

Original Instructions Rev.1.0 User Manual –Sirena 2/ 56
Explanation of Symbols
Safety alert symbol indicates a potentially hazardous situation which,
if not avoided, could result in death or serious injury
CAUTION used without the safety alert symbol indicates a potentially
hazardous situation which, if not avoided, may result in property
damage.
Safety alert symbol indicates a potentially hazardous situation which,
if not avoided, may result in minor or moderate injury.
Pinching: This warning symbol indicates the presence of a risk of
pinching when handling the lid.
Note:
Used for important information
Potential electrical hazard, only qualified person to access
Biohazard
Ground, Earth
Lead Free
Modification registration
Following modifications register updates of the manual.
Date
Revision
Modifications
Paragraph
Author
2018, october
1.0
Creation
All
F. LE CAM
CAUTION
!
CAUTION
WARNING
!


Original Instructions Rev.1.0 User Manual –Sirena 3/ 56
Table of Contents
1GENERAL INFORMATION ..........................................................................................................................5
PRESENTATION ....................................................................................................................................................5
INTENDED USE .....................................................................................................................................................5
PROHIBITIONS: ....................................................................................................................................................5
MODELS,SPECIFICATIONS AND ACCESSORIES.............................................................................................................6
1.4.1 MODELS &SPECIFICATIONS ...................................................................................................................................6
1.4.2 ACCESSORIES.......................................................................................................................................................7
SAFETY INSTRUCTIONS...........................................................................................................................................9
INFORMATION ON THE REFRIGERANT FLUID USED........................................................................................................9
2INSTALLATION INSTRUCTIONS................................................................................................................10
SHIPMENT ........................................................................................................................................................10
DAMAGED SHIPMENTS........................................................................................................................................10
HANDLING AND TRANSPORT .................................................................................................................................10
UNPACKING ......................................................................................................................................................10
PACKING LIST.....................................................................................................................................................10
MACHINE DESCRIPTION.......................................................................................................................................11
INTERFACE DESCRIPTION ......................................................................................................................................11
SITE PREPARATION..............................................................................................................................................11
ELECTRICAL &ENVIRONMENTAL REQUIREMENTS .....................................................................................................12
2.9.1 ELECTRICAL ENVIRONMENT ..................................................................................................................................12
2.9.2 ENVIRONMENTAL REQUIREMENTS .........................................................................................................................12
3OPERATING THE SIRENA CENTRIFUGE - AFI-C060 ...................................................................................13
FIRST START /POWERING....................................................................................................................................13
ROTOR ASSEMBLY ..............................................................................................................................................13
POSITION THE BUCKETS .......................................................................................................................................14
FIRST POWERING................................................................................................................................................14
ACTIVATING THE LID............................................................................................................................................14
ROTOR LOADING AND BALANCING .........................................................................................................................15
QUICK START-UP ................................................................................................................................................16
4OPERATING ............................................................................................................................................17
SETTINGS..........................................................................................................................................................17
SET SPEED ........................................................................................................................................................17
SET DURATION...................................................................................................................................................17
SET TEMPERATURE .............................................................................................................................................17
SET ACCELERATION SLOPE....................................................................................................................................18
SET BRAKING SLOPE ...........................................................................................................................................18
USE A PROGRAM TO CENTRIFUGE ..........................................................................................................................19
INTERRUPT CENTRIFUGATION................................................................................................................................19
OPEN AFTER AN ELECTRIC POWER FAILURE /EMERGENCY STOP ...................................................................................19
PRECOOLING .....................................................................................................................................................20
SETTING MODE .................................................................................................................................................20
4.11.1 SET SPEED UNIT /CENTRIFUGE FORCE ...................................................................................................................20
4.11.2 SET CENTRIFUGATION RADIUS ..............................................................................................................................21
4.11.3 SET TIME UNIT ..................................................................................................................................................21
4.11.4 SET TEMPERATURE UNIT .....................................................................................................................................22
ADVANCED PARAMETERS .....................................................................................................................................22
SLEEP MODE......................................................................................................................................................23
5CARE AND MAINTENANCE ......................................................................................................................24
CARE AND CLEANING ..........................................................................................................................................24
5.1.1 AUTHORIZED MAINTENANCE PRODUCTS..................................................................................................................24
5.1.2 CENTRIFUGE......................................................................................................................................................24
5.1.3 AUTO CLEANING FUNCTION..................................................................................................................................25
5.1.4 ROTOR &ACCESSORIES .......................................................................................................................................26
5.1.5 DISINFECTION /DECONTAMINATION......................................................................................................................26

Original Instructions Rev.1.0 User Manual –Sirena 4/ 56
DISMANTLING THE ROTOR....................................................................................................................................27
LIMITING THE DURATION OF OPERATION .................................................................................................................27
CHECKING AND TESTING ACCESSORIES ....................................................................................................................27
STORAGE OF ACCESSORIES....................................................................................................................................27
ANNUAL MAINTENANCE.......................................................................................................................................27
5.6.1 REPLACING THE BATTERY IN THE LID .......................................................................................................................28
ERROR MESSAGES,TROUBLESHOOTING,OPTION-DIAGNOSTICS..................................................................................28
REMINDER MESSAGES .........................................................................................................................................30
INFORMATIONS..................................................................................................................................................30
BACK TO MANUFACTURER....................................................................................................................................30
6DISPOSAL AND RECYCLE .........................................................................................................................31
7THEORETICAL BASICS OF CENTRIFUGATION............................................................................................32
BASICS .............................................................................................................................................................32
NOMOGRAM.....................................................................................................................................................33
LOGBOOK .........................................................................................................................................................33
RETURN COUPON ...............................................................................................................................................34
8ROTORS & ACCESSORIES.........................................................................................................................35
AFI-RX150 SWING-OUT ROTOR ..........................................................................................................................35
AFI-RHDW-150 SWING-OUT ROTOR...................................................................................................................40
AFI-RX8-5 SWING-OUT ROTOR ...........................................................................................................................41
NU-RA6-100 ANGULAR ROTOR...........................................................................................................................42
NU-RA8-50 ANGULAR ROTOR.............................................................................................................................43
NU-RA6-50 ANGULAR ROTOR ............................................................................................................................44
NU-RA10-10 ANGULAR ROTOR ..........................................................................................................................45
NU-FA8-50 ANGULAR ROTOR ............................................................................................................................46
NU-RA30-15 ANGULAR ROTOR...........................................................................................................................47
NU-RA16-5 ANGULAR ROTOR.............................................................................................................................48
NU-RA48-2 ANGULAR ROTOR.............................................................................................................................49
NU-RA30-2 ANGULAR ROTOR.............................................................................................................................50
NU-RA24-2 ANGULAR ROTOR.............................................................................................................................51
AFI-RA-PCR-8X8PCR ROTOR ............................................................................................................................52
AFI-RA-H24 HAEMATOCRIT ROTOR .....................................................................................................................53
9DECLARATION OF CONFORMITY .............................................................................................................55

Original Instructions Rev.1.0 User Manual –Sirena 5/ 56
1General Information
Présentation
The SIRENA Centrifuge is a laboratory benchtop serie of centrifuges that maximizes productivity while minimizing
laboratory required bench space. The centrifuge functionality allows the separation of substances of different densities
held in suspension or emulsion in a liquid using centrifugal force.
The SIRENA Centrifuge refrigerated model adds precise temperature control to further maximize performance.
The refrigeration system refrigerant used is CFC-free to meet regulations of refrigerant use.
Fig. 1 SIRENA Centrifuge
Intended use
The centrifuge is designed and manufactured for use with rotating accessories supplied by AFI Centrifuge. It is suitable
for separating substances of different densities held in suspension, or emulsion in a liquid.
The maximum density of substances should be 1.2 g/cm3.
The centrifuge must be used in an optimal state of operation and maintenance. The non-observance of the rule of use may
threaten the health of users or third persons, but it may also cause damages to the unit and/or surrounding equipment.
Users of the equipment must be trained on good centrifugation practices, as well specific usage of the ISA Centrifuge
models and Accessories. Any use outside the framework defined above is considered non-compliant.
AFI Centrifuge assumes no responsibility for injury due to improper use.
Prohibitions:
This centrifuge is not designed for the following uses and environments.
The following items are prohibited:
Flammable, explosive, toxic and radioactive materials
Materials which may react, causing a hazard
Contaminated materials not contained in an aerosol-barrier container
Rotating rotors and accessories that have exceeded their maximum use.
Rotating rotors and accessories that are not maintained according to the instructions in this manual, or showing
signs of wear and/or corrosion.
Rotating rotors and accessories not supplied by AFI Centrifuge.
Explosive environments
Radioactive environments
Only operate a centrifuge after taking all necessary safety measures.
Rotors and buckets should be removed from use in case of mechanical fault,
trace, or corrosion. These elements have a life cycle duration, engraved on their
visible part: To maintain safe conditions, it is imperative to replace them when
the recommended duration is reached.
CAUTION
!
CAUTION
!

Original Instructions Rev.1.0 User Manual –Sirena 6/ 56
Models, Specifications and Accessories
Specifications
Benchtop centrifuge
Reference
AFI-C060R-E
AFI- C060R
Nominal voltage (+/- 10%)
230 V
120 V
Frequency
50 Hz
60 Hz
Max. Current
5A
10A
Current phase
1
Max. Power
1100 Watt
Dimensions & Weight
Dimensions (HxWxD)
29 x 44 x 72 cm
11.4 x 17.3 x 28.4 in
Package dimension (HxWxD)
44 x 59 cm
17.3 x 23.2 in
Dimensions on benchtop
50 x 65 x 93 cm
19.7 x 25.6 x 36.6 in
Opened lid height
72 cm / 28.3 in
Weight unpacked
70 kg
Weight packed
80 kg
Centrifuge description
4.7’’ hight contrast screen

Tactile Touch

Microprocessor control

Automatic rotor recognition

Imbalance detection
Working area auto-calibration &
Continuous vibration measurement
Cycle duration
10 s to 99h : 59 min : 59 s / Continuous cycle / Pulse
Temperature units
°C / °F
Centrifugation chamber
Inox
Max. density
1.2 g / cm3
Brushless induction motor

Speed accuracy
± 0.5%
Programs number
99
Pulse (short run)

Acceleration & Braking slopes
10 & 10
Brakeless mode

Cooling system
Without CFC
Range of setting temperature
-10°C +40°C
Precool function

Temperature accuracy
+/- 2°C
Heat dissipation
3753 BTU/h
Max. noise
≤ 54 dB(A)
Refrigerant fluid / Global Warming Potential (GWP)
R452A / 2141

Original Instructions Rev.1.0 User Manual –Sirena 7/ 56
Reference
Description
Swing-Out Rotor & accessories
AFI-RX150
Swing-Out Rotor - 4x 150 ml
AFI-SB150
Square Buckets
AFI-B150
150 ml round bucket
AFI-DB50
Dedicated Buckets for 50/15 ml Conical Tube
AFI-LSB150
150 ml lid for SB150 bucket
AFI-L150
150 ml Lid for B150
AFI-RHDW-150
Swinging Bucket Rotor
AFI-RX8-5
Swinging Bucket Rotor
Adaptors for AFI-BS150 buckets
AFI-06T50C-SB
50 ml Conical Tube
AFI-06T50AOR-SB
50ml AOR* Round Tube
AFI-06T50S-SB
50 ml Skirted Conical Tube
AFI-06T30-SB
30 ml Conical Tube
AFI-06T30S-SB
30 ml Skirted Conical Tube
AFI-06T15C-SB
15ml Conical Tube
AFI-06T10C-SB
10ml Conical Tube
AFI-06T10-SB
10ml Blood Collection Tube
14ml Urine Tube
AFI-06T5-SB
5-7ml Blood Collection Tube
AFI-06T2-SB
1,5 - 2ml MicroTube
Adaptors for AFI-B150 buckets
AFI-06T80AOR
80ml AOR* Round Tube
AFI-06T50C
50 ml Conical Tube
AFI-06T50AOR
50ml AOR* Round Tube
AFI-06T50S
50 ml Skirted Conical Tube
AFI-06T30
30 ml Conical Tube
AFI-06T30S
30 ml Skirted Conical Tube
AFI-06T15C
15ml Conical Tube
AFI-06T10C
10ml Conical Tube
AFI-06T10
10ml Blood Collection Tube
14ml Urine Tube
AFI-06T5
5-7ml Blood Collection Tube
AFI-06T2
1,5 - 2ml MicroTube
Angular rotors
NU-RA6-100
6x100 ml 28° angular rotor
AFI-100R80-OAK
80ml AOR Advanced Oak Ridge
AFI-100R50-C
50ml Conical Tube
AFI-100R50-OAK
50ml AOR Advanced Oak Ridge
AFI-100R30-OAK
30ml AOR Advanced Oak Ridge
AFI-100R15-C
15ml Conical Tube
AFI-100R10-OAK
10ml AOR Advanced Oak Ridge
AFI-100R10
10ml Blood Collection Tube
AFI-100R5
5-7ml Blood Collection Tube / 5ml MicroTube
AFI-100R2
1,5 - 2ml MicroTube
NU-RA8-50
8x 50 ml 25° angular rotor
AFI-50R50-R
50ml Round Tube
AFI-50R50-OAK
50ml AOR Advanced Oak Ridge
AFI-50R30-OAK
30ml AOR Advanced Oak Ridge
AFI-50R15-C
15ml Conical Tube
AFI-50R10-OAK
10ml AOR Advanced Oak Ridge

Original Instructions Rev.1.0 User Manual –Sirena 8/ 56
AFI-50R10
10ml Blood Collection Tube
NU-RA6-50
6 x 50 ml Angular Rotor
AFI-50R50-OAK
50ml AOR* Round Tube
AFI-50R30-C
30ml AOR* Round Tube
AFI-50R30-OAK
30ml Conical Tube
AFI-50R15-C
15ml Conical Tube
AFI-50R10-OAK
10ml AOR* Round Tube
AFI-50R10
10ml Blood Collection Tube
NU-FA8-50
Angular Rotor
AFI-50R50-OAK
50ml AOR* Round Tube
AFI-50R30-C
30ml Conical Tube
AFI-50R30-OAK
30ml AOR* Round Tube
AFI-50R15-C
15ml Conical Tube
AFI-50R10-OAK
10ml AOR* Round Tube
AFI-50R10
10ml Blood Collection Tube
NU-RA30-15
30x15 ml 37° angular rotor
AFI-15R15-C
15ml Conical Tube
AFI-15R15-R
15ml Round Tube
AFI-15R10-OAK
10ml AOR Advanced Oak Ridge
AFI-15R10
10ml Blood Collection Tube
AFI-15R5
5-7ml Blood Collection Tube
NU-RA16-5
16x 5 ml 45° angular rotor
NU-RA48-2
48x 2 ml 45° angular rotor
AFI-48R05
0,5ml MicroTube
AFI-48R025
0,25ml MicroTube
AFI-48R02
0,2ml MicroTube
NU-RA30-2
30x 2 ml 45° angular rotor
AFI-30R05
0,25ml MicroTube
AFI-30R025
0,2ml MicroTube
AFI-30R02
0,5ml MicroTube
NU-RA24-2
24x 2 ml 45° angular rotor
AFI-24R05
0,5ml MicroTube
AFI-24R025
0,25ml MicroTube
AFI-24R02
0,2ml MicroTube
NU-RAPCR-8x8
8 x 8 PCR Strip Rotor
AFI-RAH-24
Hematocrit Rotor
Optional part on Refrigerated model
71110005
Cleaning kit (Sponges & Disinfectant doses)
71199003
Cleaner / Disinfectant
71199012
20gr Grease tube for accessories
71199020
Replacement Kit NU-LS150 O-rings for lids set of 4
*Advanced Oak Ridge

Original Instructions Rev.1.0 User Manual –Sirena 9/ 56
Safety Instructions
The operator must observe the following precautions when using the
centrifuge:
Ensure stability and perfect levelling of the centrifuge.
Check the correct mounting of the rotor and its accessories before starting a centrifugation cycle.
Check tube resistance at maximum applied centrifugal force: chemical resistance to centrifuged products and
mechanical strength at the centrifugal force applied thereto.
Check the condition of the tubes and remove damaged tubes.
Use only the rotors and accessories authorized by the manufacturer AFI Centrifuge.
Swing-out rotors: install four (or two) buckets; never perform any cycle with missing buckets.
Maintain and control accessories.
Balance the load of the rotor around the axis of rotation.
Respect the maximum density of 1.2 g / cm3,in particular in case of a cycle at full speed.
Limit volume in case of excess density.
Install the centrifuge in a ventilated area, on a horizontal rigid support to absorb the vibrations generated by the
centrifuge.
During operation, the centrifuge must not be moved or be subject to impact.
Do not attempt to open the lid while the rotor is spinning.
Do not attempt to neutralize the motorized lid lock
Do not lean over the centrifuge during the spin cycle.
Do not stay within a space of 10 inch around the centrifuge longer than necessary.
Do not leave potentially dangerous materials inside the free space.
Use aerosol-barrier accessories when centrifuging bio hazardous material.
Condensation may form inside the centrifuge when it is moved from a cold environment to a warm environment.
Allow the centrifuge to warm up for two hours before use.
Information on the refrigerant fluid used
The "refrigerant" type apparatus contains fluorinated greenhouse gases under the Kyoto Protocol. They should therefore
not be released into the atmosphere. Repairs or disassembly of this appliance should only be carried out by qualified
personnel.
The data corresponding to the type of refrigerant, the Global Warming Potential (GWP) value, and the load are detailed
in § §. 1.4 Specifications.
CAUTION
!

Original Instructions Rev.1.0 User Manual –Sirena 10/ 56
2Installation Instructions
Shipment
AFI Centrifuge takes every reasonable precaution to assure that your ISA Centrifuge arrives without damage. Motor
carriers are carefully selected and shipping cartons have been specially designed to insure your purchase. However,
damage can occur in any shipment and the following outlines the steps you should take on receipt of a ISA Centrifuge to
be sure that if damage has occurred, the proper claims and actions are taken immediately.
Le colis contenant la centrifugeuse nécessite d’être manipulé par des moyens mécaniques adaptés (transpalette, diable,…).
Veuillez-vous assurer que les personnes en charge de la manutention soient habilitées à manipuler le matériel de levage.
Le colis ne doit pas être placé à l'envers, ni incliné à plus de 45°.
Damaged Shipments
Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy of the
freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the basis of your
filing the claim against the carrier.
If concealed damage is found it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the carrier
will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain date and that he
failed to comply with the request. This along with other papers in the customer's possession will support the claim.
Handling and transport
The package containing the centrifuge needs to be handled by suitable means (pallet truck, etc.).
Please ensure that the people in charge of handling are qualified to handle the lifting equipment.
Maintain the following conditions during transport:
Temperature: -20 to 50 ° C
Relative humidity: <90%, under the dew point
Unpacking
Remove the cardboard packaging made of 2 parts (a lid and a bottom).
The Centrifuge was packed with great care to prevent transport hazards. Before disposing of the packaging of the
centrifuge, and its spinning accessories, please make sure you are in possession of the following:
Packing list
SIRENA Centrifuge
Compliance Certificate
User Manual
Food grade grease
Power cord
Manual release key
CAUTION
!

Original Instructions Rev.1.0 User Manual –Sirena 11/ 56
Machine Description
Fig. 1 ¾ Front view
1. Lid
2. Window
3. Power switch button
4. Control panel
5. Opening lid button
6. Cooling unit
7. Electrical outlet
Interface description
1. Power ON-OFF, START-STOP, & cycle interruption
button. Can be activated through single press or press &
hold action.
2. Setting cursor / Slider
3. Display screen
4. Touch bar for validation after parameters modification.
Can be activated through single press or press & hold
action.
5. Color flashing zone. The circular zone is sensible to
finger swipe to the right direction.
6. Program number
7. Service key logo
8. Speed parameters : unit (rpm or xg), radius (mm)
9. Time unit : Hour / Minute or Minute / Second
10.Temperature unit (Celsius or Fahrenheit degree)
11.Slope parameters : Acceleration or Braking slope
Site preparation
Prepare a stable, clean and levelled support (e.g. laboratory bench). This support must be able to support the weight of
the centrifuge and its use, and be firm enough so as not to generate or maintain vibration. Such vibrations would degrade
the results of the centrifugation, and may result in an unbalanced error.
A clearance envelope of 12 inches (300 mm) around the centrifuge
should be arranged according to IEC 61010-2-020: No potentially
hazardous material, nobody or no object shall be deposited within the
free space.
Fig. 2 Open space around the centrifuge
It is recommended that no less than two people are present to manually lift the
centrifuge onto the laboratory bench.
2
1
3
5
4
7
6
2
1
3
4
5
6
7
8
9
10
11
CAUTION
!

Original Instructions Rev.1.0 User Manual –Sirena 12/ 56
Electrical & Environmental Requirements
The centrifuge requires must be 230V/50Hz or 120V/60Hz, single phase, (current rating varies per centrifuge model)
according to the manufacturer sheet on the back of the unit.
The centrifuge must be plugged into an outlet with protective earthing connection with the standard power cord.
The electrical outlet into which the centrifuge is connected should be readily accessible for maintenance purposes.
The power cord plug serves as the main disconnect device and should remain readily accessible. The electrical outlet
should be on its own branch circuit, protected with a circuit breaker at the distribution panel near the centrifuge.
The centrifuge is designed for use under the following ambient conditions (according to EN 61010-1)
• Indoor use
• Maximum altitude: 2000 m
• Ambient temperature range of 5 to 40 ° C
• Maximum relative humidity of 80% for temperatures up to 22 ° C,
• Supply voltage fluctuation <± 10% of rated voltage.
• Overvoltage on the Category II power supply network (IEC 60364-4-44 standard)
• Maximum degree of pollution of the room: 2.0
• Walls less than 1m away must be able to stop the centrifuge from moving in the event of a major accident.
Maximum performance is ensured in the following ambient temperature range: 15 ° C to 25 ° C.

Original Instructions Rev.1.0 User Manual –Sirena 13/ 56
3Operating the SIRENA centrifuge - AFI-C060
First Start / Powering
The first start must fulfill the conditions of the IEC 61010-2-020 safety standard
HOW TO GREASE A SIRENA CENTRIFUGE
Rotor Assembly
Clean and lubricate the motor shaft by depositing a thin film of the food grade grease
supplied.
Place the rotor above the drive shaft.
Slide the rotor on the drive shaft.
A click indicates that the rotor is locked.
Clean and lubricate the pins by depositing a thin film of the food grease supplied
Grab the synthetic grease container provided with your centrifuge.
Dispense a small amount of grease on each of the following locations:
At first start, or when
following message appears:
+ 01

Original Instructions Rev.1.0 User Manual –Sirena 14/ 56
Position the buckets
Swing-out rotors are used with round and square buckets or microplate buckets.
Place the buckets & lids in the corresponding numbered locations.
First powering
After transport, wait at least 2 hours before putting the appliance
into operation so that the refrigerant circuit is fully efficient.
Connect the power cord to the mains.
Press & maintain the START-STOP button onto the control panel,
until display powering.
An animation starts during the initialization phase.
The parameters of a program appear.
Note: To enter the standby mode, press and hold the START /
STOP key: Only the START/STOP button remains illuminated.
Activating the lid
To open the lid:
Turn on the centrifuge.
Press the opening lid button on the right side of the control block.
The lid is unlocked and lifts automatically.
Note :
Lid opening is also available from OK zone :
Swipe hand over OK zone : The lid opens.
During a first start, it is possible that the lid does not open frankly after prolonged storage. In this case, activate the
spring hinges by manually manipulating the cover from top to bottom.
To close the lid, lower the lid and press until click.
CAUTION

Original Instructions Rev.1.0 User Manual –Sirena 15/ 56
Rotor loading and balancing
Position the 4 numbered buckets in their corresponding positions on the rotor. Each
bucket should pivot freely in its slot.
Place the inserts corresponding to the tubes to be centrifuged in the four buckets.
The buckets must be symmetrically distributed.
For better balance, each numbered bucket must be placed in the corresponding position
marked on the rotor.
Each position on the rotor must be equipped with its bucket.
Do not use a rotor without its complete set of four buckets.
Maximum loads according to the type of rotor are specified in the annex.
The centrifuge tolerates an imbalance of 8 grams.
If the imbalance is higher than the tolerated threshold, an imbalance detection system
stops the centrifuge automatically.
Key logo and error message "02" appear. The rotor stops in free wheel mode without
braking system.
Excessive imbalance is likely to damage the turning components and/or the centrifuge.
Follow loading instructions referring to corresponding rotor description in § 8
Rotors & accessories.
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Original Instructions Rev.1.0 User Manual –Sirena 16/ 56
Quick start-up
Before using the centrifuge, make sure it has been properly installed.