Agilent Technologies 1260 Infinity II User manual

Agilent Technologies
Agilent 1260 Infinity II
High Temperature
Sample Preparation
System
User Manual

Revision 10.0 November 2016
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Health and Safety
Agilent 1260 Infinity II High Temperature Sample Preparation System
The use of this instrument does not entail any hazard if used in accordance with the instructions
stated within the Operator’s manual. The protection provided by this equipment may be impaired if
it is used with accessories not provided or recommended by Agilent or used in a manner not
specified in this manual.
Users should observe the following safety precautions:
Ensure that all the instructions within the Operator’s manual are understood and carried out in the
operation of the instrument. All persons utilising the instrument should have adequate training in
its proper set-up, operation, and particularly its safety features.
Voltages above 210V AC are present within the instrument, and access covers should not be
removed by anyone other than properly trained service personnel. No attempt should be made to
service the instrument without authorisation from Agilent’s service department and contravention
of this may result in personal hazard or damage to the instrument and will invalidate the
manufacturer's warranty.
We stress the importance of standard safe practice (such as COSHH regulations) for dealing with
electronic laboratory equipment, solvents, etc., in preventing accidents, fires, or potentially
hazardous conditions.
The individual or group responsible for the use and maintenance of this equipment must ensure
that appropriate decontamination is carried out if hazardous material is spilt on or inside the
instrument.
Before using any cleaning or decontamination method except those recommended by Agilent, Inc.,
users should check with Agilent that the proposed method will not damage the equipment. See
Section 6 for detailed recommended procedures.
If in any doubt about the use of the instrument contact your local Agilent office or local distributor.

Contents
NOTICES II
HEALTH AND SAFETY III
1GENERAL INFORMATION 1
1.1 Overview 1
1.2 Specifications 1
1.3 Unpacking 2
1.4 Location 2
1.5 Utility Requirements 3
1.6 Safety Precautions 3
2SYSTEM OVERVIEW 5
3INSTALLING THE MODULE 8
3.1 Preparing the system 8
4SYSTEM OPERATION 9
4.1 Power On 9
4.2 Shaker 9
4.3 Temperature Controller 12
4.4 Safety Over-Temperature Cut-Out 13
4.5 Hand Pipettor 14
5SAMPLE PREPARATION 15
5.1 Procedure 15
5.2 Cleaning the Frits 16
6CLEANING AND DECONTAMINATION PROCEDURE 17
7MAINTENANCE 18
SAFETY STANDARDS 20
ABSTRACT 21

Agilent 1260 HT Sample Prep System Operation Manual
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1General Information
1.1 Overview
Agilent’s high temperature sample preparation system is designed for routine dissolution and filtration
of polymer solutions at temperatures up to 260 °C. Controlled heating and careful shaking of the
samples assists dissolution, and a unique hand pipettor device, fitted with an interchangeable frit
magazine, efficiently filters and dispenses the hot solution directly into autosampler vials with minimal
handling. The vials then can be crimped and transferred directly into the high temperature carousel.
1.2 Specifications
Type
Specification
Temperature Range
Ambient to 260 °C
Temperature Accuracy
±2 °C
Temperature Control
PI Control with RTD temperature sensor
Number of Samples
20
Capacity of preparation vial
minm2 mLs maxm20 mLs
Mode of sample agitation
Horizontal motion controlled via on/off switch with variable speed control
Sample transfer
Hand held pipettor
Filtration
0.5, 2, 5, 10 µm stainless steel frit or 1 µm glass fibre disk
Safety
Overvoltage category II
Pollution degree 2
Max. operating altitude 2000 m
Indoor use only
Independent over-temperature cut-out
Power Requirements:
210-250V AC, line frequency 50/60Hz
(max. voltage fluctuation ± 10 %).
See rating plate on instrument.
Environmental Conditions:
10 °C –30 °C,
< 95% rH, non-condensing
Dimensions:
Packaged size
505 x 585 x 205 mm (w x d x h)
1000 x 600 x 500 mm (w x d x h)
Weight:
Packaged weight
40kg, 88lbs.
45kg, 99lbs

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1.3 Unpacking
Care has been taken to ensure that the instrument should be received in proper condition. The
packing and protection are designed for normal hazards of road, rail or air transit. Any damages to
the container or instrument should be reported immediately to your local distributor or to Agilent,
Inc. It is recommended that the shipping container be kept, if possible, for re-shipment or return to a
service centre.
Examine the shipping box for visible signs of exterior damage. Unpack the instrument and examine
for transit damage. Refer to local manual lifting guidelines and regulations. It is recommended that
instrument be lifted by a minimum of two people. Check that all items on the packing list are
included.
Notify your local distributor or Agilent of any damage or missing items.
This instrument is heavy (40kg)
1.3.1 Packing list
Sample Preparation System
Hand pipettor
10 x Filter magazines fitted with 2 µm frits
1 pack of sample preparation vials (20 mL, 100)
1 pack of aluminium crimp caps (100)
2” Exhaust ducting (2M)
2” Jubilee clip
4 mm nylon tubing for gas purge (2M)
1.4 Location
The Sample Preparation System must be supported by a sturdy, level bench. Preferably
avoid exposure to direct sunlight.
The unit must be sited in a fume cupboard or suitably extracted environment when used with
hazardous and dangerous chemicals.
Provide adequate space around the unit to allow for the motion of the horizontal shaker.

Agilent 1260 HT Sample Prep System Operation Manual
3
Ensure that the power switch & appliance power connector situated at the rear of the unit
remain easily accessible at all times.
Do not use this equipment in hazardous atmospheres or with hazardous materials for which it
was not designed.
1.5 Utility Requirements
1.5.1 Electrical Power
Single phase mains power is required. The instrument is designed for connection to a Cat II
installation.
Before plugging in the power cable, ensure the instrument voltage rating matches your local
power supply.
Use only a supply with protective grounding.
The correct fuses should be fitted:
230V (AC): 2x 250V 5A F HBC 20 mm fuses (inlet)
1x 250V 5A FF HBC (heater).
RISK OF FIRE, REPLACE FUSES AS MARKED!
THE UNIT IS DOUBLE-FUSED. DISCONNECT POWER CORD BEFORE
REMOVING COVERS!
1.5.2 Extraction
The unit should be positioned in a fume hood or extracted area. Additional extraction via a 2” duct
may also be required
1.5.3 Nitrogen Supply
The hood of the unit is fitted with a 4 mm push-fit gas pipe coupling. Nitrogen purge gas should be
fed into the hood at approx 1SLM.
1.6 Safety Precautions
1.6.1 Condensation within the Module
Condensation will damage the system electronics.
➔ Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔ If your module was shipped in cold weather, leave it in its box and allow it to
warm slowly to room temperature to avoid condensation.

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1.6.2 Preventative Maintenance
Periodically inspect the power lead for signs of wear or damage. Contact Agilent’s Service
Department to obtain a replacement lead –it must be fitted with a ferrite to maintain EMC
compatibility.
1.6.3 Temperature
The Sample Preparation System is designed to control the temperature of the sample preparation
vials to temperatures up to 260 °C and therefore the metal hot plates, vials and the base of the hand
pipettor will be extremely hot during normal operation. Please handle these components carefully
wearing suitable protective gloves. Heat sensitive warning labels are affixed to the hot sections of
the instrument for your protection.
Beware Hot Surfaces
1.6.4 Toxic and Flammable Vapours
To prevent the risk of accumulation of toxic and/or harmful vapours, the system must be used in a
fume cupboard or suitably extracted area when operated at elevated temperatures and with organic
solvents. Additional vapour removal can be achieved by connecting the hood of the hot plate to an
extraction system via standard 2”(50 mm) flexible ducting.
1.6.5 Risk Reduction Procedures for Flammable Liquids
THE FOLLOWING PRECAUTIONS ARE EXTREMELY IMPORTANT
Keep any sources of ignition (e.g. sparks or naked flames) well away from hot solvents!
Always wear a grounded anti-static wrist strap when manipulating flammable liquids.
Ensure the temperature of solvents is always kept at least 10 °C below their boiling point.
Always use extraction or ventilation to minimise the concentration of flammable vapours.
Remove solvent reservoir containers and cover all vials before shaking.
The hood of the unit should be purged with Nitrogen during shaking.
Reduce the exposed surface area of solvents to a minimum by replacing vial caps and container
lids as quickly as possible.
Replace the hood whenever possible.
Do not overfill containers –allow plenty of room for solvent motion during shaking.
Use the minimum necessary quantity of solvents.
Mop up any spillages immediately.

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2System Overview
The Agilent 1260 Infinity II High Temperature Sample Prep System is illustrated in figures 2.1a and 2.1b. It
comprises: -
A horizontal shaker [A]
Temperature controlled hot blocks [B] for sample dissolution in 20 mL vials.
The temperature control and shaking cycles are control from the keypad.[C]
The shaker speed is adjusted using the potentiometer [D]
Independent overtemperature safety cut-out system [E]
Power switch [F - located at the rear of the unit]
Removable extraction hood [G figure 2.1b] creating an oven environment for the samples and
has facilities for a nitrogen purge and vapour extraction.
Interchangeable blocks [H], the blocks with which contain the 2 mL vials are reversible
revealing a hole pattern for 4 mL autosampler vials.
A hand held pipettor [I] which is heated in the hot block when not in use.
Interchangeable frit magazines [J figure 2.2]
Figure 2.1a System Overview
B
C
E
D
C
A
B
F
C
I
H
D
E

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Figure 2.1b System Overview
G